CN103598098B - Pachyrhizua angulatus tissue culturing fast seedling-cultivating method - Google Patents
Pachyrhizua angulatus tissue culturing fast seedling-cultivating method Download PDFInfo
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Abstract
The present invention relates to Plant Tissue Breeding propagation technique field, a kind of Pachyrhizua angulatus tissue culturing fast seedling-cultivating method, comprise the steps: (1) pretreatment; (2) foundation of sterilizable material system; (3) Fiber differentiation; (4) propagation of bud; (5) culture of rootage; (6) acclimatization and transplants; Pachyrhizua angulatus tissue culturing fast seedling-cultivating method of the present invention, application method for tissue culture, effectively can keep the stable character of excellent female parent.Compare with propagation by layering with the cuttage of routine, tissue cultures with less excellent female parent, can breed large batch of seedling.At one time in section (60d), each female parent of cuttage can only breed an effective seedling, and the reproduction coefficient of tissue cultures is 20 ~ 30 times of cuttage.
Description
Technical field
The present invention relates to Plant Tissue Breeding propagation technique field, a kind of Pachyrhizua angulatus tissue culturing fast seedling-cultivating method.
Background technology
Pachyrhizua angulatus (Puerariaelobatavar.Thomsonii), for pulse family (Leguminosae) Pueraria lobota belongs to (Pueraria) plant.Have 8 kinds and 2 mutation in China, be mainly distributed in the west and south, the middle and south to the southeast, North of Yangtze River is rare.The edible part of Pachyrhizua angulatus is block root, is commonly called as " root of kudzu vine ".
The root of kudzu vine is the mountain region plant of the medicine-food two-purpose that the Ministry of Public Health of China is assert.Root of kudzu vine taste is sweet, pungent, and property is put down, and has relieving muscles to expel heat, promotes the production of body fluid, the merit of the positive antidiarrheal of promoting eruption, liter; Puerarin (isoflavonoid) content is high, has reducing blood pressure, reduces myocardial consumption of oxygen, reducing heart rate, expansion hat blood shape pipe improve metabolism that is normal and ischemic myocardium, improve the effect such as Brain circlulation and peripheral circulation.Along with the development of modern medicine, food science and technology, deepening continuously of the pharmacology, medicine, clinical practice, food development research etc. of root of kudzu vine fermentation, its use value more and more causes the concern of people, is described as " Asia ginseng ".Authoritative institution's predictions such as the World Food Programme, near 5,000 ten thousand tons/year of global root of kudzu vine demand (amounting to 2,000 ten thousand mu).
In addition, Radix Pueraria thomsonii starch content is high, and some improved seeds content of starch can produce alcohol fuel 1kg up to the fresh Pachyrhizua angulatus of 25%, 7.5kg.The alcohol production rate Pachyrhizua angulatus in unit are soil is higher than the cereal crops such as corn, sweet potato, and Pachyrhizua angulatus biological nitrogen fixation efficiency is high, impoverishment tolerant, has the advantage fully excavating soil potential.Pachyrhizua angulatus, as non-grain living beings new material, avoids the bottleneck of " strive grain with the people, strive ground with grain ", has huge value of exploiting and utilizing.
Daba Mountain Pachyrhizua angulatus (Puerarialobata (wild) Ohwi'Dabashan') is the breeding of being tamed from wild Pueraria kind by Dazhou City forestry science and technology centre for spreading, 2010 by the forest variety certification committee of Sichuan Province authorization (accession designation number is: river R-WTS-PL-023-2009), become the Variety comprehensive that Qin_Ba mountain areas development Pachyrhizua angulatus industry is promoted mainly, development area rapid development is large to seedling demand.
The breeding of current Daba Mountain Pachyrhizua angulatus is mainly based on press strip and cottage propagation conventional method, and reproduction coefficient is low, the seedling limited amount of acquisition, cannot meet the demand that current agricultural is produced, how provide the seedling of a large amount of high-quality to become current problem demanding prompt solution.
That to be Modern Agricultural biotechnology on producing most widely used is general for Plant Tissue Breeding Fast-propagation, produce a maximum field of economic benefit.Tissue-culturing quick-propagation seedling has the superiority of its uniqueness, first reproduction coefficient is large, expanding propagation can be carried out with thousands of speed to millions of times faster than conventional method, a large amount of high quality seedling is provided in time, a new kind is met the needs of production at short notice, accelerates to introduce a fine variety the process of the popularization with breeding; The propagating materials used is little and few; Breeding nursery stock neat and consistent, commodity is high, can keep the good characteristic of original kind; Can carry out anniversary factorial seedling growth, production efficiency is high.Low to some reproduction coefficient, can not, with the breeding of the plant variety of seminal propagation (occurring kind of a decline for property without seed or seminal propagation offspring), utilize tissue-culturing quick-propagation even more important.
At present, deliver although existing Pueraria lobota belongs to Jingxi district Pueraria lobota, Pachyrhizua angulatus, U.S. kudzu, the tissue cultivating and seedling technical patent of Thailand Pueraria lobota and the several kind of elegant jessamine and report, but different plant species, between even same species, different cultivars, its method for tissue culture also has very large otherness.Daba Mountain Pachyrhizua angulatus is new varieties, also there is no the report of tissue culturing fast seedling-cultivating.And also there are this some problems in Pachyrhizua angulatus tissue cultures: draw materials and be difficult to ensure maternal hereditary quality in field; On Pachyrhizua angulatus kudzu, fine hair is many, and pollution rate is high, is difficult to set up sterile system; The rate of increase is low.Therefore, seek and explore Daba Mountain Pachyrhizua angulatus tissue-culturing quick-propagation seedling growing process, being expand Daba Mountain Pachyrhizua angulatus provenance to produce, solving the urgent task of seedling demand in producing.
Summary of the invention
The object of the invention is to provide a kind of Pachyrhizua angulatus tissue cultivating and seedling method, utilizes tissue culture technique, improves the reproduction coefficient of Pachyrhizua angulatus, cultivates the Pachyrhizua angulatus clone of stabilization characteristics of genetics.
For achieving the above object, the technical solution used in the present invention is: a kind of Pachyrhizua angulatus tissue culturing fast seedling-cultivating method, comprises the steps:
(1) excellent strain block root carries out potted plant by pretreatment, is placed in green house, treats that it sends bud tendril and grows to 20cm, starts to spray 1% carbendazim solution, once in a week, continues one and a half months, starts to draw materials when tendril grows to 1m;
(2) fecula Pueraria lobota kudzu is then chosen in the foundation of sterilizable material system, cut off blade and petiole, cut into the stem section that 1.0 ~ 1.5cm long band has axillalry bud, 30min is rinsed under stem with bud being placed in running water, then 10 ~ 30min is soaked with detergent liquid, scrub under stem Duan Houzai puts flowing water with soft brush and rinse 1 ~ 2h, on superclean bench, be 75% alcohol-pickled 15 ~ 20s by the stem section mass concentration of having washed, aseptic water washing 2 ~ 4 times, then 3 ~ 5min is soaked with the mercury chloride of 0.1%, aseptic water washing uses 2% clorox process 8 ~ 10min for 4 ~ 6 times again, aseptic water washing 4 ~ 6 times, blot stem with bud with filter paper and obtain sterilizable material,
(3) the aseptic stem with bud that above-mentioned steps obtains by Fiber differentiation is first inoculated on 1/2MS medium, 10 ~ 15d is cultivated under dark condition, reduce brownization to occur, then the sprouting and growth that bud inducement medium promote axillalry bud is proceeded to, by illumination and interlunation than the Fiber differentiation that hockets for 2:1;
(4) the propagation bud inducement of bud cultivates 25 ± 2d, and axillary bud sprouting grows to 1 ~ 1.5cm, it is all proceeded to proliferated culture medium and repeatedly breeds, and proceeds to culture of rootage after obtaining a large amount of propagation seedlings;
(5) bud that culture of rootage chooses 2 ~ 3cm from Multiplying culture carries out culture of rootage;
(6) plantlet in vitro of culture of rootage takes out from culturing room by acclimatization and transplants, is moved on to by blake bottle in greenhouse to carry out high light and close bottle and practice seedling 6 ~ 8d, then opens bottleneck, in bottle, adds PP
333with carbendazim mixed liquor, prevent germ contamination and promote strong sprout, then placing 7 ~ 10d, after hardening completes, with tweezers, test-tube plantlet is taken out gently, put into clear water basin, carefully wash away root agar, then flood goes out, with transplanting in Nutrition Soil after carbendazim process, Nutrition Soil upper strata is 2cm river sand, and lower floor is 4cm perlite, vermiculite and humus mixture, seedling nursery measure management routinely after having young leaves to grow.
Further: described Pachyrhizua angulatus is selected from Daba Mountain Pachyrhizua angulatus.
Further: the inducing culturing condition of described step (2) is: temperature 24 ~ 26 DEG C, light intensity 2500lx, humidity 70%.
Further: the bud inducement medium of described step (3) contains MS basal medium+0.5 ~ 1.0mg/L6-BA+0.1 ~ 0.3mg/LIBA+ sucrose 30g/L+ agar 7.0g/L.
Further: the proliferated culture medium of described step (4) contains MS basal medium+0.3 ~ 0.5mg/L6-BA+0 ~ 0.1mg/LIBA+ sucrose 30g/L+ agar 7.0g/L.
Further: the root media of described step (5) contains 1/2MS basal medium+0 ~ 0.1mg/LIBA+ sucrose 30g/L+ agar 7.0g/L.
Further: the pH of described medium is 5.8 ~ 6.0.
Further: during described step (6) acclimatization and transplants, air themperature is 20 ~ 25 DEG C, humidity is 85%.
In the present invention, 1/2MS refers to that the macroelement in MS medium reduces by half, and other all remain unchanged.
Advantageous Effects of the present invention is: Pachyrhizua angulatus tissue culturing fast seedling-cultivating method of the present invention, and application method for tissue culture, effectively can maintain the stable character of excellent female parent.Compare with propagation by layering with the cuttage of routine, tissue cultures with less excellent female parent, can breed large batch of seedling.At one time (60d) in section, each female parent of cuttage can only breed an effective seedling, the reproduction coefficient of tissue cultures be cuttage 20 ~ 30 times.
Embodiment
Below in conjunction with concrete embodiment, the present invention will be further described;
Embodiment one
A kind of Pachyrhizua angulatus tissue culturing fast seedling-cultivating method, comprises the steps:
(1) excellent for Daba Mountain Pachyrhizua angulatus strain block root carries out potted plant by pretreatment, is placed in green house, treats that it sends bud tendril and grows to 20cm, starts to spray 1% carbendazim solution, once in a week, continues one and a half months, starts to draw materials when tendril grows to 1m;
(2) fecula Pueraria lobota kudzu is then chosen in the foundation of sterilizable material system, cut off blade and petiole, cut into the stem section that 1.0cm long band has axillalry bud, 30min is rinsed under stem with bud being placed in running water, then 10min is soaked with detergent liquid, scrub under stem Duan Houzai puts flowing water with soft brush and rinse 1h, on superclean bench, be 75% alcohol-pickled 150s by the stem section mass concentration of having washed, aseptic water washing 2 times, then 3min is soaked with the mercury chloride of 0.1%, aseptic water washing uses 2% clorox process 8min for 4 times again, aseptic water washing 4 times, blot stem with bud with filter paper and obtain sterilizable material,
(3) the aseptic stem with bud that above-mentioned steps obtains by Fiber differentiation is first inoculated on 1/2MS medium, 10d is cultivated under dark condition, reduce brownization to occur, then upper sprouting and the growth promoting axillalry bud of bud inducement medium (inducing culture contains MS basal medium+0.5mg/L6-BA+0.1mg/LIBA+ sucrose 30g/L+ agar 7.0g/L) is proceeded to, by illumination and interlunation than the Fiber differentiation that hockets for 2:1, cultivation temperature 24 DEG C, light intensity 2500lx, humidity 70%;
(4) propagation of bud cultivates 23d at bud inducement, axillary bud sprouting grows to 1cm, it is all proceeded to proliferated culture medium (proliferated culture medium contains MS basal medium+0.3mg/L6-BA+0.1mg/LIBA+ sucrose 30g/L+ agar 7.0g/L) and carry out Multiplying culture repeatedly, after obtaining a large amount of propagation seedlings, proceed to culture of rootage;
(5) bud that culture of rootage chooses 2cm from Multiplying culture carries out culture of rootage (root media contains 1/2MS basal medium+0.1mg/LIBA+ sucrose 30g/L+ agar 7.0g/L);
(6) plantlet in vitro of culture of rootage takes out from culturing room by acclimatization and transplants, blake bottle is moved on in greenhouse and carry out high light and close bottle and practice seedling 6d, then bottleneck is opened, place 3d again, after hardening completes, with tweezers, test-tube plantlet is taken out gently, put into clear water basin, carefully wash away root agar, then flood goes out, with transplanting in Nutrition Soil after carbendazim process, Nutrition Soil upper strata is 2cm river sand, and lower floor is 4cm perlite, vermiculite and humus mixture, and air temperature control is at 20 DEG C, humidity is 85%, seedling nursery measure management routinely after having young leaves to grow.
Above-mentioned medium used all adopts HCl and NaOH of 1mol/L to regulate medium pH to be 5.8 ~ 6.0.
Embodiment two
A kind of Pachyrhizua angulatus tissue culturing fast seedling-cultivating method, comprises the steps:
(1) excellent for Daba Mountain Pachyrhizua angulatus strain block root carries out potted plant by pretreatment, is placed in green house, treats that it sends bud tendril and grows to 20cm, starts to spray 1% carbendazim solution, once in a week, continues one and a half months, starts to draw materials when tendril grows to 1m;
(2) fecula Pueraria lobota kudzu is then chosen in the foundation of sterilizable material system, cut off blade and petiole, cut into the stem section that 1.3cm long band has axillalry bud, 30min is rinsed under stem with bud being placed in running water, then 20min is soaked with detergent liquid, scrub under stem Duan Houzai puts flowing water with soft brush and rinse 1.5h, on superclean bench, be 75% alcohol-pickled 18s by the stem section mass concentration of having washed, aseptic water washing 3 times, then 4min is soaked with the mercury chloride of 0.1%, aseptic water washing uses 2% clorox process 9min for 5 times again, aseptic water washing 5 times, blot stem with bud with filter paper and obtain sterilizable material,
(3) the aseptic stem with bud that above-mentioned steps obtains by Fiber differentiation is first inoculated on 1/2MS medium, 13d is cultivated under dark condition, reduce brownization to occur, then upper sprouting and the growth promoting axillalry bud of bud inducement medium (inducing culture contains MS basal medium+0.7mg/L6-BA+0.2mg/LIBA+ sucrose 30g/L+ agar 7.0g/L) is proceeded to, by illumination and interlunation than the Fiber differentiation that hockets for 2:1, cultivation temperature 25 DEG C, light intensity 2500lx, humidity 70%;
(4) propagation of bud cultivates 25d at bud inducement, axillary bud sprouting grows to 1.2cm, it is all proceeded to proliferated culture medium (proliferated culture medium contains MS basal medium+0.4mg/L6-BA+0.1mg/LIBA+ sucrose 30g/L+ agar 7.0g/L) and carry out Multiplying culture repeatedly, after obtaining a large amount of propagation seedlings, proceed to culture of rootage;
(5) bud that culture of rootage chooses 2.5cm from Multiplying culture carries out culture of rootage (root media contains 1/2MS basal medium+0.1mg/LIBA+ sucrose 30g/L+ agar 7.0g/L);
(6) plantlet in vitro of culture of rootage takes out from culturing room by acclimatization and transplants, blake bottle is moved on in greenhouse and carry out high light and close bottle and practice seedling 7d, then bottleneck is opened, place 5d again, after hardening completes, with tweezers, test-tube plantlet is taken out gently, put into clear water basin, carefully wash away root agar, then flood goes out, with transplanting in Nutrition Soil after carbendazim process, Nutrition Soil upper strata is 2cm river sand, and lower floor is 4cm perlite, vermiculite and humus mixture, and air temperature control is at 20 DEG C, humidity is 85%, seedling nursery measure management routinely after having young leaves to grow.
Above-mentioned medium used all adopts HCl and NaOH of 1mol/L to regulate medium pH to be 5.8 ~ 6.0.
Embodiment three
A kind of Pachyrhizua angulatus tissue culturing fast seedling-cultivating method, comprises the steps:
(1) excellent for Daba Mountain Pachyrhizua angulatus strain block root carries out potted plant by pretreatment, is placed in green house, treats that it sends bud tendril and grows to 20cm, starts to spray 1% carbendazim solution, once in a week, continues one and a half months, starts to draw materials when tendril grows to 1m;
(2) fecula Pueraria lobota kudzu is then chosen in the foundation of sterilizable material system, cut off blade and petiole, cut into the stem section that 1.5cm long band has axillalry bud, 30min is rinsed under stem with bud being placed in running water, then 30min is soaked with detergent liquid, scrub under stem Duan Houzai puts flowing water with soft brush and rinse 2h, on superclean bench, be 75% alcohol-pickled 20s by the stem section mass concentration of having washed, aseptic water washing 4 times, then 5min is soaked with the mercury chloride of 0.1%, aseptic water washing uses 2% clorox process 10min for 6 times again, aseptic water washing 6 times, blot stem with bud with filter paper and obtain sterilizable material,
(3) the aseptic stem with bud that above-mentioned steps obtains by Fiber differentiation is first inoculated on 1/2MS medium, 15d is cultivated under dark condition, reduce brownization to occur, then upper sprouting and the growth promoting axillalry bud of bud inducement medium (inducing culture contains MS basal medium+1.0mg/L6-BA+0.3mg/LIBA+ sucrose 30g/L+ agar 7.0g/L) is proceeded to, by illumination and interlunation than the Fiber differentiation that hockets for 2:1, cultivation temperature 24 DEG C, light intensity 2500lx, humidity 70%;
(4) propagation of bud cultivates 27d at bud inducement, axillary bud sprouting grows to 1.5cm, it is all proceeded to proliferated culture medium (proliferated culture medium contains MS basal medium+0.5mg/L6-BA+0.1mg/LIBA+ sucrose 30g/L+ agar 7.0g/L) and carry out Multiplying culture repeatedly, after obtaining a large amount of propagation seedlings, proceed to culture of rootage;
(5) bud that culture of rootage chooses 3cm from Multiplying culture carries out culture of rootage (root media contains 1/2MS basal medium+0.1mg/LIBA+ sucrose 30g/L+ agar 7.0g/L);
(6) plantlet in vitro of culture of rootage takes out from culturing room by acclimatization and transplants, blake bottle is moved on in greenhouse and carry out high light and close bottle and practice seedling 8d, shade density is 70%, then bottleneck is opened, place 7d again, after hardening completes, with tweezers, test-tube plantlet is taken out gently, put into clear water basin, carefully wash away root agar, then flood goes out, with transplanting in Nutrition Soil after carbendazim process, Nutrition Soil upper strata is 2cm river sand, lower floor is 4cm perlite, vermiculite and humus mixture, air temperature control is at 20 DEG C, humidity is 85%, seedling nursery measure management routinely after having young leaves to grow.
Above-mentioned medium used all adopts HCl and NaOH of 1mol/L to regulate medium pH to be 5.8 ~ 6.0.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (4)
1. a Pachyrhizua angulatus tissue culturing fast seedling-cultivating method, is characterized in that: comprise the steps:
(1) excellent strain block root carries out potted plant by pretreatment, is placed in green house, treats that it sends bud tendril and grows to 20cm, starts to spray 1% carbendazim solution, once in a week, continues one and a half months, starts to draw materials when tendril grows to 1m;
(2) fecula Pueraria lobota kudzu is then chosen in the foundation of sterilizable material system, cut off blade and petiole, cut into the stem section that 1.0 ~ 1.5cm long band has axillalry bud, 30min is rinsed under stem with bud being placed in running water, then 10 ~ 30min is soaked with detergent liquid, scrub under stem Duan Houzai puts flowing water with soft brush and rinse 1 ~ 2h, on superclean bench, be 75% alcohol-pickled 15 ~ 20s by the stem section mass concentration of having washed, aseptic water washing 2 ~ 4 times, then 3 ~ 5min is soaked with the mercury chloride of 0.1%, aseptic water washing 4 ~ 6 times, use 2% clorox process 8 ~ 10min again, aseptic water washing 4 ~ 6 times, blot stem with bud with filter paper and obtain sterilizable material,
(3) the aseptic stem with bud that above-mentioned steps obtains by Fiber differentiation is first inoculated on 1/2MS medium, 10 ~ 15d is cultivated under dark condition, reduce brownization to occur, then the sprouting and growth that bud inducement medium promote axillalry bud is proceeded to, by illumination and interlunation than the Fiber differentiation that hockets for 2:1;
(4) the propagation bud inducement of bud cultivates 25 ± 2d, and axillary bud sprouting grows to 1 ~ 1.5cm, it is all proceeded to proliferated culture medium and repeatedly breeds, and proceeds to culture of rootage after obtaining a large amount of propagation seedlings;
(5) bud that culture of rootage chooses 2 ~ 3cm from Multiplying culture carries out culture of rootage;
(6) plantlet in vitro of culture of rootage takes out from culturing room by acclimatization and transplants, is moved on to by blake bottle in greenhouse to carry out high light and close bottle and practice seedling 6 ~ 8d, then opens bottleneck, in bottle, adds PP
333with carbendazim mixed liquor, prevent germ contamination and promote strong sprout, then placing 7 ~ 10d, after hardening completes, with tweezers, test-tube plantlet is taken out gently, put into clear water basin, carefully wash away root agar, then pull out, with transplanting in Nutrition Soil after carbendazim process, Nutrition Soil upper strata is 2cm river sand, and lower floor is 4cm perlite, vermiculite and humus mixture, seedling nursery measure management routinely after having young leaves to grow;
The bud inducement medium of described step (3) contains MS basal medium+0.5 ~ 1.0mg/L6-BA+0.1 ~ 0.3mg/LIBA+ sucrose 30g/L+ agar 7.0g/L;
The proliferated culture medium of described step (4) contains MS basal medium+0.3 ~ 0.5mg/L6-BA+0 ~ 0.1mg/LIBA+ sucrose 30g/L+ agar 7.0g/L;
The root media of described step (5) contains 1/2MS basal medium+0 ~ 0.1mg/LIBA+ sucrose 30g/L+ agar 7.0g/L;
Described Pachyrhizua angulatus is selected from Daba Mountain Pachyrhizua angulatus.
2. Pachyrhizua angulatus tissue culturing fast seedling-cultivating method according to claim 1, is characterized in that: the inducing culturing condition of described step (3) is: temperature 24 ~ 26 DEG C, light intensity 2500lx, humidity 70%.
3. Pachyrhizua angulatus tissue culturing fast seedling-cultivating method according to any one of claim 1, is characterized in that: the pH of described medium is 5.8 ~ 6.0.
4. Pachyrhizua angulatus tissue culturing fast seedling-cultivating method according to claim 1, it is characterized in that: during described step (6) acclimatization and transplants, air themperature is 20 ~ 25 DEG C, humidity is 85%.
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Families Citing this family (10)
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|---|---|---|---|---|
| CN103988779A (en) * | 2014-05-22 | 2014-08-20 | 四川农业大学 | Efficient corn callus seedling regeneration culture method |
| CN104839024A (en) * | 2015-05-18 | 2015-08-19 | 凤阳金小岗农林科技产业发展有限公司 | Tissue culture seedling-exercising method |
| CN105613299A (en) * | 2016-02-05 | 2016-06-01 | 王少荣 | Method for eliminating bacterial contamination of chrysanthemum tissue cultured seedlings |
| CN105660402A (en) * | 2016-02-05 | 2016-06-15 | 王少荣 | Method for saving Jiyichao chrysanthemum tissue culture seedling from mold contamination |
| CN106172004A (en) * | 2016-07-31 | 2016-12-07 | 张玉薇 | The method for quickly breeding of Radix Puerariae |
| CN108012794A (en) * | 2017-12-05 | 2018-05-11 | 湖北黄仙洞葛业食品有限公司 | A kind of pueraria lobata implantation methods |
| CN109362550A (en) * | 2018-12-18 | 2019-02-22 | 湖北省农业科学院中药材研究所 | A kind of pueraria lobata water planting method for culturing seedlings |
| CN110583483B (en) * | 2019-09-30 | 2022-05-17 | 贵州大学 | Method for inducing cluster buds of pachyrhizua angulatus |
| CN110731266B (en) * | 2019-11-27 | 2020-06-23 | 广西壮族自治区农业科学院 | Simple and efficient radix puerariae polyploid induction method |
| CN111149704B (en) * | 2020-03-13 | 2023-04-14 | 广西壮族自治区农业科学院 | Proliferation and one-step seedling culture method for single-bud stem of pachyrhizua angulatus |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1164831B1 (en) * | 1999-03-25 | 2003-11-05 | University of Guelph | Micropropagation and production of phytopharmaceutical plants |
| CN1899027A (en) * | 2002-11-04 | 2007-01-24 | 杨凌润地生态科技有限公司 | Tissue culture quick breeding seedling producing method for kudzu vine |
-
2013
- 2013-11-19 CN CN201310585519.6A patent/CN103598098B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1164831B1 (en) * | 1999-03-25 | 2003-11-05 | University of Guelph | Micropropagation and production of phytopharmaceutical plants |
| CN1899027A (en) * | 2002-11-04 | 2007-01-24 | 杨凌润地生态科技有限公司 | Tissue culture quick breeding seedling producing method for kudzu vine |
Non-Patent Citations (6)
| Title |
|---|
| avone-producing Puerarialobata (Willd.) Ohwi.《Plant Science》.2003,第165卷第1123-1128页. * |
| Barbara Thiem etal.In vitropropagationofisofl * |
| Kamlesh Vaishnav etal.isoflavonoids production in callus culture of Pueraria tuberosa,the indian kudzu.《indian journal of expermental biology》.2006,第44卷第1012-1017页. * |
| 刘红昌等.野葛组织培养的研究进展.《时珍国医国药》.2005,第16卷(第10期),第1037-1039页. * |
| 张帆等.粉葛试管块根诱导技术的研究.《时珍国医国药》.2008,第19卷(第4期),第918-919页. * |
| 黄宁珍等.泰国葛组织培养和快速繁殖体系优化研究.《中国中药杂志》.2008,第33卷(第19期),第2175-2179页. * |
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