CN201015341Y - Intermittent immersion type plant incubators - Google Patents

Intermittent immersion type plant incubators Download PDF

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Publication number
CN201015341Y
CN201015341Y CNU2007200120545U CN200720012054U CN201015341Y CN 201015341 Y CN201015341 Y CN 201015341Y CN U2007200120545 U CNU2007200120545 U CN U2007200120545U CN 200720012054 U CN200720012054 U CN 200720012054U CN 201015341 Y CN201015341 Y CN 201015341Y
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China
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incubator
plant
liquid
connected
solenoid valve
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CNU2007200120545U
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Chinese (zh)
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琳 李
李超峰
杨柏珍
王力华
赵望锋
邓正正
韩桂云
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中国科学院沈阳应用生态研究所
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/25Greenhouse technology
    • Y02A40/256Lighting systems for greenhouses
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/25Greenhouse technology
    • Y02A40/264Devices or systems for heating, ventilating, regulating temperature, or watering
    • Y02A40/266Collecting solar energy
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/25Greenhouse technology
    • Y02A40/264Devices or systems for heating, ventilating, regulating temperature, or watering
    • Y02A40/268Air-conditioning systems
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/10Agricultural machinery or equipment
    • Y02P60/14Measures for saving energy
    • Y02P60/146Measures for saving energy in greenhouses
    • Y02P60/147Heating, ventilation or air conditioning
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/20Reduction of greenhouse gas [GHG] emissions in agriculture
    • Y02P60/21N2O
    • Y02P60/212Reducing the use of fertilizers
    • Y02P60/216Aquaponics or hydroponics

Abstract

The utility model relates to an intermittent immersion plant incubator, comprising an incubator which is the main device that the plant organizes the liquor to cultivate, a lamp housing whose inner has an illuminating lamp arranged at the top of the incubator and used for lighting the internal incubator and providing the illumination the plant is needed, and a nutrient solution storage container which is connected with the incubator and used for providing nutrient solution needed when planting; wherein the incubator comprises a working chamber, a regulation chamber and a control device; a liquor culture vessel, air supply pipes, a temperature and humidity sensor, a water level sensor and a gas concentration sensor are arranged in the working chamber; the air supply pipe is connected with the regulation chamber through air inlet pipe, and a liquor ascending total pipe and a liquor returning total pipe are arranged between the liquor culture vessel and the nutrient solution storage container, an upstream valve and a pump are arranged at the liquor ascending total pipe, and a liquor returning solenoid valve is arranged at the liquor returning total pipe; and each sensors above are connected with the control device. The utility model has the advantages of saving the energy consumption, realizing the large-scale production, simplifying the plant acclimation procedure and reducing the cost.

Description

一种间歇浸没式植物培养箱 An intermittent immersion plant incubator

技术领域 FIELD

本实用新型涉及一种生物技术领域中植物培养的装置,具体地说是一种间歇浸没式植物培养箱。 The present invention relates to an apparatus for plant culture in the field of biotechnology, specifically a batch submerged plant incubator. 背景技术 Background technique

植物组织培养技术自诞生至今,广泛应用于作物育种、花卉、果树、蔬菜、林木及中草药的脱毒和快繁方面,具有其他技术手段无法比拟的优势, 已成为现代生物技术不可或缺的技术领域。 Plant tissue culture technology from the birth date, widely used in crop breeding, flowers, fruit, vegetables, trees and herbs and rapid propagation of virus-free terms, with other techniques can not match advantage, has become an indispensable technology of modern biotechnology field. 为了实现组培高效、低成本工厂化育苗,国内外许多学者就组培技术本身的改进展开了一系列研究,如培养容器和培养基质的替代、非试管苗快繁、无糖培养、液体培养,及最近几年的培养微环境调控等。 Tissue culture in order to achieve efficient, low-cost factory breeding, many researches on the improvement of the technology itself launched a series of tissue culture studies, such as the culture vessel and the culture alternative substrates, non-vitro rapid propagation, sugar culture, liquid culture and train control and other micro-environment in recent years. 而高效的商业化快繁依靠的是快速大规模繁殖及组培苗木的高质量。 And efficient commercialization of large-scale propagation relies on rapid propagation and tissue culture of high-quality wood. 组培程序的机械化和自动化,能有助于其商业化应用。 Tissue culture procedures of mechanization and automation, can contribute to its commercial application. 显然, Obviously,

液体培养在实现组培程序的自动化方面首当其选,而且从1994年起,欧盟国家在COST822和COST843计划(European Cooperation in the field of Scientific and Technical Research)(欧洲科技领域研究合作组织)支持下,连续11年对液体培养、生物反应器技术、适宜材料识别、系统集成以及相关生理学、发育学方面的内容进行了广泛研究,其中有代表性的成果是提出并研制了间歇式气-液交换生物反应器如气升式(RITA⑧(W096/25484))和双瓶式(BIT⑧)生物反应器,两者的特点是:l)能够保持良好的通风透气,2)连续培养减少了继代次数;3)反应器物化条件可以调控;4)省却难以循环回收的基质物料;5)再生植株抗逆性显著增强,从而有效降低了人力物力成本。 Liquid Culture automation to achieve tissue culture program first when their election, and since 1994, EU countries COST822 and COST843 program (European Cooperation in the field of Scientific and Technical Research) (European Research Cooperation in science and technology) support under 11 years, liquid culture, bioreactor technology, suitable materials identification, system integration and physiologically related, aspects of learning content development has been extensively studied, which is representative of the results proposed and developed intermittent gas - liquid exchange the airlift bioreactor formula (RITA⑧ (W096 / 25484)) and bis jar (BIT⑧) bioreactor, both characteristics are: l) able to maintain good ventilation, 2) reducing the frequency in continuous culture generations ; 3) the reaction conditions can be regulated objects; 4) recycling difficult to dispense matrix materials; 5) significantly enhanced resistance regenerated plants, thus effectively reducing the cost of manpower and resources. 但气体驱动液体流动使能耗上升、气体驱动不稳定,培养液更新操作复杂,导致环境敏感型(养分和激素)物种发育困难,并且RITA® (the Recipient for Automated Temporary Immersion System,自动化间歇浸没系统培养容器)结构复杂使制造成本过高, BIT®( the Twin flasks system,双瓶式系统)培养瓶冗余使单位空间利用率降低, 又限制了它们的普遍应用。 However, gas-driven liquid flow increased energy consumption, gas driven unstable broth update complex operation, resulting in environmentally sensitive (nutrient and hormone) species development difficult, and RITA® (the Recipient for Automated Temporary Immersion System, automated batch immersion system culture vessel) the manufacturing cost is too high structural complexity, BIT® (the Twin flasks system, two-bottle systems) flasks redundancy reduction per unit space utilization, but also limits their widespread use. 实用新型内容 SUMMARY

为了克服上述培养箱结构成本不能控制的缺点,本实用新型的目的在于提供一种能够自动调控、改善培养微环境条件、实现液体间歇供应养分,组培植物的增殖和再生的一种用于植物组织液体培养的装置。 In order to overcome the shortcomings of the incubator can not control the cost structure, object of the present invention is to provide an automatic control, to improve the culture microenvironment, intermittent supply of nutrients to achieve a liquid, tissue culture proliferation and regeneration of plants for plant liquid tissue culture apparatus.

为实现上述目的,本实用新型采用的技术方案是: 一种间歇浸没式植物培养箱,包括:培养箱,为植物组织液体培养的主要装置; To achieve the above object, the technical solution of the present invention is that: a submerged intermittent plant incubator, comprising: an incubator, the main device is a liquid plant tissue culture;

灯箱,内部具有照明灯,设于培养箱的上部,对培养箱内部的照明,提供植物所需的光照; Boxes, with interior lighting, provided on the upper portion of the incubator, the incubator of the internal illumination to provide the required illumination plants;

营养液存储容器与培养箱连接,为培养箱提供培养植物所需的营养液。 Nutrient solution storage container is connected with an incubator, the incubator is required to provide a plant nutrient solution culture.

所述培养箱包括工作室、调挺仓及控制装置,工作室内部具有液体培养 The incubator comprises a chamber, and a control device adjustment quite the cartridge, the interior chamber having a liquid culture

容器、供气管,还设有温/湿度传感器、水位传感器、以及气体浓度传感器; 供气管通过进气管接至调控仓,液体培养容器与营养液存储容器之间设有上液总管路及回液总管路,上液总管路上设有逆水闽和水泵,回液总管路上设 Container, feed pipe, also a temperature / humidity sensor, a water level sensor and a gas concentration sensor; feed pipe connected to the intake pipe regulated by the cartridge, is provided on the liquid culture fluid manifold and the liquid return path between the container and the nutrient solution storage vessel manifold passage, the liquid against the current road at Min and manifold pumps liquid return manifold is provided on the road

有回液电磁阀;上述各传感器与控制装置相连。 There liquid return solenoid valve; of the sensors connected to the control device.

所述调控仓内具有加湿器水箱,该加湿器水箱底部设有风道,风道外壁 The modulator having a water tank cartridge humidifier, the humidifier is provided with air duct bottom of the tank, the outer wall of duct

上安装有加热/制冷装置;所述风道的进风口通过风机引入空气,出风口经过一电磁阀连接至工作室的供气管中。 Mounted heating / cooling means; said air passage introducing air through the fan inlet, the outlet through a solenoid valve connected to the working chamber of the air supply pipe.

所述电磁阀为四通电磁阀,其第一进气口与风道的出风口相连,第一出气口通过进气管接至工作室的供气管;第二出气口接回至加湿器水箱中,四通电磁阀的第二进气口与加湿器的水箱相连通。 The four-way solenoid valve is a solenoid valve having a first inlet and outlet duct is connected through an intake duct of the first outlet duct connected to the supply chamber; a second outlet connected back to the humidifier tank , and the second intake port of the humidifier tank through the four-way solenoid valve is connected.

加湿器水箱内壁上具有光触媒涂层,加湿器水箱内具有发光电极。 Humidifier water tank having a photocatalyst layer on an inner wall, an electrode having a light emitting humidifier tank.

本实用新型具有以下优点: The present invention has the following advantages:

1. 节省能耗:本实用新型与已有的气体驱动在间歇培养供应养分期间靠连续气体驱动来保持培养物与养分的接触方式,耗能高,相比本实用新型提出 1. energy saving: the present invention and conventional gas-driven manner against the contact in batch culture during the continuous gas-driven supply of nutrients to maintain the culture with nutrient, high energy consumption, compared to the present invention proposes

的液体驱动是靠水泵,通过电磁阀的开关调节养分与培养物的接触时间,变气体驱动为液体驱动,节省能耗;利用储液罐内安装的消毒措施或在培养液中附加消毒剂进行系统消毒,方便快捷,省却了高压灭菌所带来的高能耗。 Is driven by liquid pump, regulating contact time with the nutrient culture by switching the solenoid valve, the liquid becomes a gas driven driving, energy saving; disinfection measures using the reservoir tank installed in a culture broth or additional disinfectant disinfection system, convenient, save the autoclave brought about by high energy consumption.

2. 可以规模化生产:本实用新型通过微环境条件的调控,提高组培物的质量,使外植体达到最佳的生长、分化和代谢状态,整体设计为箱体结构,并且不受时间和地点的限制,可以随时随地组织规模化生产。 2. Can scale production: The present invention is regulated by the microenvironment, improve the quality of the Tissue Culture of the explants to achieve optimal growth, differentiation and metabolic state, overall design of a box structure, and is not time and site constraints, anytime, anywhere can organize large-scale production.

3. 本实用新型为提高植物驯化成活率装置,由于微环境可调,因而可以用调节C02和02浓度的方法提供植物生长所需的碳源,取代在培养基中附加糖作为碳源,从而建立正确的碳的库源关系,也为植物向自养方向发展创造了条件。 3. The present invention is a means to improve the survival rate of domesticated plants, since the micro-environment variable, it is possible to provide a desired plant growth regulating method of a carbon source concentration of C02 and 02, additional substitution of sugar as a carbon source in the medium, whereby establish the correct relationship between the carbon source libraries, but also to create the conditions for the plant to develop self-support direction.

5.简化植株驯化程序,降低成本。 The simplified plant acclimation procedures and reduce costs. 现有技术中用凝胶固体培养基组培诱导生成的根,是气生根,因为凝胶固体培养基中含有非气体相,因而在驯化过程中,原有的根在驯化基质中不起作用,只有重新长出新鲜根才能驯化成活。 Gel with the prior art solid medium tissue culture induced to root, root is air, because the gel-solid medium containing non-gaseous phase, and thus in the process of domestication, the root does not work in the original matrix acclimatization , only to re-grow fresh roots domesticated survived. 而用液体间歇培养,诱导生成的根,简短驯化后,就可以栽到温室或大棚地里,简化植株驯化程序,降低成本,而且培养系统保持良好的通风换气系统, 因而所培养的植株抗逆性显著增强,提高了组培苗驯化成活率,从而有效降低了生产成本,并且具备现有技术中气升式培养箱的所有优点。 The liquid batch culture, induced to root, after a brief acclimation, can be planted to a greenhouse or in the shed, domesticated plants simplified procedures, reduce costs, and the culture system to maintain a good ventilation system and therefore resistant plants cultured inverse significantly enhanced to improve the survival rate of tissue culture acclimation, thereby reducing the production cost effectively, and have all the advantages of the prior art airlift incubator.

附图说明 BRIEF DESCRIPTION

图l为本实用新型总体结构示意图; Figure l of the present invention generally schematic configuration utility;

图2为本实用新型调控仓及工作室结构示意图; FIG 2 is a schematic view of the new regulation and the cartridge chamber configuration utility;

图3为利用本实用新型进行培养的结构示意图; FIG 3 is a schematic view of the present invention by culturing;

图4为本实用新型控制电路原理图; FIG 4 is a control circuit diagram invention;

图5为本实用新型控制过程流程图(一); FIG 5 is a flowchart of the control process invention (a);

图6为本实用新型控制过程流程图(二)。 FIG 6 is a flowchart showing the control process invention (II).

附图符号说明如下:l.培养箱,2.工作室,3.营养液存储容器,4.底架, REFERENCE SIGNS follows:.... L incubator chamber 2, 3 nutrient solution storage container, the chassis 4,

M2.水泵,6.控制装置,QNC.气体浓度传感器,8.营养液管道,WDC/SDC.温/ 湿度传感器,IO.加湿器,ll.调控仓,U.灯箱,13.风道,14.液位显示计, 15.加热/制冷组件,16.出风口, DL四通电磁阀,171.第一进气口, 1".第一出气口, 173.第二出气口, 174.第二进气口, 18.进气管,19.供气管,20. 排气孔,21.进气孔,Ml.风机,22.过滤网,23.液体培养容器,24.逆水闽, 25.上液总管路,D3.回液电磁阀,SWC.水位传感器,28.回液总管路。 具体实施方式 M2. Pumps, 6. The control apparatus, QNC. Gas concentration sensor, 8. nutrient solution pipe, WDC / SDC. Temperature / humidity sensors, the IO. Humidifier, LL. Regulatory positions, the U-. Boxes, 13. Duct 14 liquid level display meter 15. the heating / cooling assembly 16. outlet, the DL-way solenoid valve 171. the first intake port 1. "the first outlet, a second outlet 173., 174. the first two intake port 18. the intake pipe, 19 gas supply pipe 20 vent 21 into the hole, of Ml. blower 22. filter 23 liquid culture container 24. Min against the current, the 25 fluid manifold passage, D3. liquid return solenoid valve, the SWC. water level sensor 28. The liquid return manifold passage. DETAILED DESCRIPTION

下面结合附图和实施例对本实用新型进行详细说明: The present invention is described in detail below in conjunction with the accompanying drawings and examples:

如图l、图2、图3所示,本实用新型包括:培养箱l、灯箱12及营养液存储容器3,其中培养箱1为3个,分三层安装于一底架4上部的柜体内,为培养植物组织液体的主要装置;灯箱12内部具有照明灯,设于培养箱l的上部,对培养箱l内部进行照明,可抽拉式灯箱12为植物提供所需的光照;营养液存储容器3,设于培养箱1底部,为培养箱1提供培养植物所需的营养液; 可抽拉式灯箱】2其内具有的照明日光灯。 FIG. L, FIG. 2, FIG. 3, the present invention comprises: an incubator L, boxes 12 and nutrient solution reservoir 3, wherein the incubator 1 is 3, three sub-chassis 4 mounted to an upper portion of a cabinet in vivo, for the main culture liquid plant tissue means; box having interior illumination lamp 12, provided on the upper portion of the incubator l, l of the incubator interior is illuminated, the pull-out box 12 to provide the required illumination plants; nutrient solution storage container 3, is provided at the bottom of the incubator 1, the incubator 1 to provide desired plant nutrient solution culture; drawable formula 2] boxes having therein a fluorescent lighting.

本实施例采用3个以不同高度置放于底架4上培养箱l,其中每个培养箱1的工作室中具有3个液体培养容器23,每个液体培养容器23均通过上液总管路25及回液总管路28与营养液存储容器3相连。 The present embodiment uses three different heights placed on the chassis 4 L incubator, the incubator 1, wherein each chamber having three liquid culture vessels 23, 23 are each liquid culture vessel through the fluid manifold passage 25 and the liquid return manifold passage 28 connected to the storage container 3 nutrient solution.

所述培养箱1包括工作室2、调控仓11及控制装置6,工作室2内部具有液体培养容器23、供气管19,还设有温/湿度传感器WDC/SDC、气体浓度传感器QNC以及水位传感器SWC,上述各传感器与一控制装置6相连;供气管19通过进气管路18接至调控仓11,液体培养容器23与营养液存储容器3之间设有上液总管路25及回液总管路28,上液管路包括上液总管路25及上/回液支管,上液总管路25上设有水泵M2及逆水阀24,回液管路包括上/回液支管及回液总管路28,回液总管路28上设有回液电磁阀D3。 The incubator 1 includes a chamber 2, the regulation of the cartridge 11 and a control device 6, the second internal chamber having a liquid culture container 23, supply tube 19, is also a temperature / humidity sensor WDC / SDC, gas concentration sensor and a water level sensor QNC the SWC, each of the sensors connected to a control device 6; regulated air supply pipe 19 connected to cartridge 11 through intake line 18, manifold provided with fluid passage 25 and the liquid return path between the manifold 3 and a liquid nutrient solution culture vessel storage vessel 23 28, the fluid conduit comprises a fluid passage 25 and the manifold / liquid return branch pipe, with the valve 24 against the current pump M2 and the manifold passage 25 to the liquid, the liquid return conduit includes / liquid return branch pipe 28 and the liquid return manifold passage , the liquid return passage 28 is provided with the liquid return manifold solenoid valve D3.

本实施例中工作室2为长方体形状,工作室2内四角处设有四个立柱形的供气管19,四个供气管19通过通风管18、四通电磁闳Dl与调控仓11内的加湿器10水箱相通;工作室2内设有设有C02气体浓度传感器QNC,工作室2还具有C02混合气体的进气孔21及排气孔20; C02气体瓶与混合气体瓶连通,并与高压空气在混气瓶内混合经设有电磁阀的气管与工作室2进气孔21连通,通过气体流量计调节气体流量提供所需浓度的C02混合气体,控制培养箱l内混合气体浓度。 Embodiment 2 In the present embodiment chamber rectangular parallelepiped shape, the inner chamber 2 is provided with four corners 19, four column-shaped humidifying the feed pipe 19 through a feed pipe four vent tube 18 within the four-way solenoid Hong Dl cartridge 11 and control water tank 10 in communication; C02 chamber 2 features a gas concentration sensor provided QNC, chamber 2 further includes a mixed gas intake hole 21 and C02 exhaust hole 20 is; C02 gas cylinder in communication with the mixed gas bottle, and with the high pressure air mixture within the cylinder intake port 21 communicates with the mixing tube through the working chamber 2 of the solenoid valve, C02 provide the desired concentration of the mixed gas, the mixed gas concentration in the incubator controls l adjusting the gas flow rate through the gas flow meter.

本实施例中调控仓11内设有加湿器10、风机M1、风道13、四通电磁阀Dl,所述加湿器10的水箱底部设有风道13,风道13外壁上安装有加热/制冷组件15 (本实施例采用陶瓷片),风机M1通过过滤网22设置在风道13 — 端的进风口处,自然空气在风机M1的作用下经风道13进行加热或制冷;所述风道13的进风口通过风机M1连续的引入自然空气,当不需要加湿的时候, 出风口16经过四通电磁阀Dl的第一进气口171及第一出气口172由进气管18连接至工作室2的供气管19,改变工作室2内的温度;所述四通电磁阀Dl, 当需要加湿的时候,空气在风机M1的作用下经风道13进行加热或制冷,出 In this embodiment the cartridge 11 equipped with the regulation of the humidifier 10, the fan M1, air duct 13, the four-way solenoid valve Dl, the bottom of the tank 10 is provided with the humidifier air duct 13, an outer wall of the duct 13 is mounted with a heating / refrigeration assembly 15 (the present embodiment uses a ceramic plate), the fan M1 through the filter 22 disposed at 13 duct - air inlet end, natural air is heated or cooled via the duct 13 under the action of the fan M1; said duct air inlet 13 is introduced through the continuous natural air fan M1, when not required humidified, the air outlet 16 through the four-way solenoid valve Dl of the first intake port 171 and a first outlet 172 is connected to the working chamber 18 by the intake pipe 2 supply pipe 19, to change the temperature in the chamber 2; Dl of the four-way solenoid valve, when humidification is required, the cooling or heating air through the duct 13 under the action of the fan M1, the

风口16经过四通电磁阀Dl的第一进气口171其第二出气口173接回至加湿器10水箱中,加湿器IO水箱输出端与四通电磁阀Dl的第二进气口174相连通,空气加湿后经过四通电磁阀Dl的第二进气口174及第一出气口172由进气管18连接至工作室2的供气管19,改变工作室2内的温湿度;调控仓U 内的加湿器IO采用超声波加湿器;加湿器IO水箱内壁上设有具有消毒作用的光触媒涂层,加湿器10水箱内具有发光电极。 16 through the first air intake port 171 of the four-way solenoid valve Dl its second outlet 173 back to the tank 10 to the humidifier, the humidifier is connected to the output terminal IO tank Dl the four-way solenoid valve 174 of the second intake port pass, the air humidified through the four-way solenoid valve Dl second intake port 174 and a first outlet 172 is connected to the working chamber 18 by the intake pipe supply pipe 192, changing temperature and humidity inside the chamber 2; U regulation cartridge IO ultrasonic humidifier in the humidifier; photocatalyst layer is provided with a disinfectant on the inner wall of the tank IO humidifier, the humidifier 10 has a light emitting electrode within the tank. 光触媒在发光电极的照射产生具有超高氧化能力的氢氧自由基(OH)和氧负离子(02-),并与有害气体结合成无害的二氧化碳和水;风机M1及四通电磁阀Dl与控制装置连接。 Hydroxyl radical (OH) and oxygen ions (02-), and combine them into harmless carbon dioxide and water and harmful gases photocatalyst is irradiated with ultra-high oxidizing power is generated in the light emitting electrode; M1 and the four-way solenoid valve fan Dl and connection control means.

所述营养液存储容器3设置于底架4下部,通过上液总管路25及多个上/回水支管分别与多个液体培养容器23连接,在上液总管路25上设有水泵M2及逆水阀24,各个上/回水支管同时通过回液总管路28接回至营养液存储容器3,该回液总管路28上设有回液电磁阀D3;液体培养容器23内设水位传感器SWC,用于检测水位信息,当需要营养液回流时,液体培养容器23 通过控制装置6开启控制回液电磁阀D3,经上/回水支管及回液总管路28回流到营养液存储容器3中;本实用新型还可在储液罐10处设有消毒装置,其消毒装置可为紫外灯、臭氧发生器、光触媒、微过滤器等。 The nutrient solution storage container 3 is disposed in a lower portion of the chassis 4, the upper fluid through manifold passage 25 and a plurality of return branch pipes are connected to the plurality of culture container 23 of liquid / liquid manifold passage is provided in the pump 25 and the M2 against the current valve 24, each of the upper / back return branch pipe while connected to the passage 28 through the liquid return manifold 3 nutrient solution storage container, the liquid return manifold passage 28 is provided on the liquid return solenoid valve D3; culture vessel 23 equipped with a liquid level sensor SWC , 3 for detecting the water level information, when the nutrient solution needs to reflux, liquid culture container 23 is opened the liquid return control solenoid valve D3 by the control device 6, the warp / return branch pipe 28 and the liquid return manifold passage to the nutrient solution was refluxed storage vessel ; present invention may also be provided with reservoir sterilizing apparatus or the like, which disinfection apparatus may be a UV lamp ozone generator, photocatalyst, at the microfilter 10.

控制装置6设于调控仓11的控制面板上,其电源插头及数据传输接口设于调控仓ll的一侧,控制面板上具有控制按钮SA. SB. Sc. Sd. SE及显示器xp, 其中控制装置6可通过数据传输RS-485接口与远程计算机进行通讯连接; The control device 6 is provided on the control panel 11 of the cartridge-control, the power plug and the data transfer interface disposed on a side of the cartridge regulation ll having control buttons SA. SB. Sc. Sd. SE xp, and a display on the control panel, wherein the control data transmission means 6 may be an RS-485 interface communication connection with the remote computer;

如图4所示,所述控制装置6具有单片机U1,该单片机U1的通讯管脚A、 B、通过数据传输接口与远程计算机进行通讯连接,该数据传输接口可为RS-485接口, RS-232接口, RS-422接口,蓝牙接口或红外接口,本实施例采用RS-485接口;单片机U1的输入端分别接有各控制按钮SA. SB. Sc. SD. SE、 水位传感器SWC、温度/湿度传感器WDC/SDC及气体浓度传感器QNC;单片机的输出端分别通过第1开关SI接至制冷/加热组件15,通过第2开关接至灯箱12中的日光灯L,通过第3开关S3分别接至适度控制四通电磁阀Dl 、 冷热温度控制电磁阀D2、水泵M2、回液电磁阀D3、进气电磁阀D4的线圈及风机M1。 As shown, the control device 6 has a microcontroller U1 4, the communications microcontroller U1 pin A, B, communicate via a data transmission interface with a remote computer, the data transmission interface may be RS-485 interface, RS- 232 interface, RS-422 interface, a Bluetooth interface or an infrared interface, the present embodiment uses RS-485 interface; input of the microcontroller U1 are connected with the respective control buttons SA SB Sc SD SE, the water level sensor the SWC, temperature /.... a humidity sensor WDC / SDC and the gas concentration sensor QNC; the output of the microcontroller, respectively through the first switch SI is connected to the cooling / heating assembly 15, via a second switch connected to the fluorescent lamp L in box 12, connected to via a third switch S3 are appropriate control of the four-way solenoid valve Dl, cold temperature control solenoid valve D2, pump M2, the liquid return solenoid valve D3, D4 of the inlet solenoid valve and fan coil M1.

如图5所示,本实用新型通过以下步骤实现其功能: 5, the present invention achieves its functions by the following steps:

开机,控制装置初始化;设定环境变量及控制参数;判断上述控制参数中的控制参数执行时间是否结東; Power control means for initializing; setting environment variables and control parameters; determining a control parameter of the control parameters if the execution time of the east junction;

如没有结東,则控制装置采集并显示各传感器检测信号;控制装置将传感器检测信号与控制参数进行比较; If no junction east, the control device acquiring and displaying a detection signal of each sensor; the control means compares the sensor detection signal and a control parameter;

如果传感器检测信号没有满足控制参数要求,控制装置输出控制信号启动相应的执行机构进行处理,存储检测信息;如果传感器检测信号满足控制参数要求,存储检测信息; If the sensor detection signal does not meet the requirements of the control parameters, the control means outputs a control signal to the appropriate actuator starts processing, storing detection information; if the sensor detects parameters required to meet the control signal, storing the detected information;

判断控制过程是否结東;如没有结東信息,则返回判断上述控制参数的执行时间是否结束阶段,否则结束控制过程;如果判断上述控制参数的执行时间是否结東的结果为是,则返回至设定控制参数阶段。 Determining control process is complete. The East; if no junction East information, determines the control parameters of the execution time is returned if the end phase, otherwise the control process is terminated; if the result of determination of the control parameters of the execution time is the junction east is YES, the process returns to stage set control parameters.

所述设置控制参数包括具有n套(n为自然数,l《n《50,本实施例中n=10,)每套控制参数包括培养植物所需的温度及湿度、所需气体浓度、按植物生长所需光照设置光照的时间、按植物生长所需设置营养液的液位高度及液位保持时间以及每套控制参数执行时间。 The control parameter includes setting sleeve having n (n is a natural number, l "n" 50, in the present embodiment, n = 10,) each include control parameters required for plant cultivation temperature and humidity, the desired gas concentration, according to the plant lighting settings required for growth of the illumination time provided by the plant growth nutrient solution and the level of the liquid level and the hold time each time execution control parameters.

所述控制装置对传感器检测信号及控制参数进行比较包括液位控制过程、气体浓度控制过程、温/湿度控制过程、光照时间控制过程;其中液位控 Said control means detects the sensor signals and control parameters comprises comparing the level control process, gas concentration control process, temperature / humidity control process, the illumination time control process; wherein the level control

制过程包括以下步骤:判断液体培养容器内液位是否达到设定高度;进入高水位时间段,如没达到设定高度,则启动水泵给液体培养容器添加培养液至设定高度;判断高水位时间段是否结束;如果没有结束,则继续保持高水位; 如果有结束,则打开回水电磁阀,进入低水位时间段;判断低水位时间是否结東,如果没有结束,则继续保持低水位时间段;如果结東,则进入高水位时间段,实现高低液位的循环供应液体。 Molding process comprising the steps of: determining whether the liquid level of the culture vessel reaches a set height; enters the high level period, as did not reach the predetermined height, the culture vessel to start the pump to the liquid culture medium was added to a set height; Analyzing high level time period has ended; If not, it continues to maintain a high level; if ended, the solenoid valve is opened backwater into the low level period; low level time is determined east node, if not ended, the time to maintain a low water level segment; East If the junction, the process proceeds to the high level period and low level to achieve the supply of liquid circulation.

所述气体浓度控制过程包括以下步骤:判断C02气体浓度是否高于设定浓度;如果高于设定浓度,则排出气体;如果低于设定浓度,则吹入C02气体。 The gas concentration control process comprising the steps of: determining the concentration of C02 gas is higher than the set concentration; if it exceeds the set concentration, the exhaust gas; if less than the set concentration, the C02 gas is blown.

所述温/湿度控制过程包括以下步骤:判断温/湿度是否高于设定温/湿度; 如果高于设定温度,则启动制冷组件,制冷吹入的空气;如果低于设定温度, 则启动加热组件,加热吹入的空气;如果高于设定湿度,则风机吹入自然的空气;如果低于设定湿度则启动加湿装置,加湿吹入湿的空气。 The temperature / humidity control procedure comprises the steps of: determining whether the temperature / humidity higher than the set temperature / humidity; if it exceeds the set temperature, the cooling assembly is started, the cooling air is blown; if less than the set temperature, start heating assembly, heating the blown air; if higher than the set humidity, the nature of the fan air is blown; the set humidity is less than the starting humidifier, humidifying humid air is blown.

如图6所示,为本实用新型提供的一种用于植物组织液体培养的装置中进行培养的方法,包括以下步骤: 6, the present invention provides a method for plant tissue culture apparatus in a liquid culture comprising the steps of:

按控制按钮,设定环境变量及控制参数,系统巡检时间为30秒,检测一次传感器环境参数,并传递给单片机,单片机将当前环境参数与目标控制参数进行对比;控制装置6输出控制信号启动相应的执行机构进行处理,执行机构包括风机M1、水泵M2、加湿器10、加热/制冷组件15、照明灯以及各电磁阀;所述控制装置6对传感器信号及控制参数进行比较包括液位控制过程、气体浓度控制过程、温/湿度控制过程、光照时间控制过程。 Press control button to set the environment variables and control parameters, system inspection time of 30 seconds, a sensor detecting environmental parameters, and passed to the microcontroller, the microcontroller comparing the current environmental parameters and target control parameter; 6 startup control means outputs a control signal processing respective actuator, the actuator comprises a fan M1, pumps M2, the humidifier 10, the heating / cooling assembly 15, lights and solenoid valves; six pairs of the control signals and the sensor control parameters comprises comparing the level control means process, gas concentration control process, temperature / humidity control process, the illumination time control process.

开机,控制装置初始化;设定环境变量及控制参数,调用EEROM内的第n套(n为自然数,l《n《50,本实施例中n=10)控制参数循环信息;开始时间暗室;执行第1循环的目标环境参数:预定控制时间为5天;设定温度25。 Power control means for initializing; setting environment variables and control parameters, call the n sets in the EEROM (n is a natural number, l "n" 50, in this embodiment n = 10) control parameters loop information; start time darkroom; performed certain environmental parameters in the first cycle: a predetermined control time is 5 days; 25 set temperature. C;湿度40。 C; humidity 40. /。 /. RH; C02气体浓度为700ppm;光照时间10h,暗时间14h; 高水位时间lh,低水位时间6h;手动设置水位传感器高度为5cm; RH; C02 gas concentration of 700 ppm; irradiation time 10h, 14H dark time; time high water lh, low water 6H time; manually set the height of the water level sensor 5cm;

保持温度稳定在25土2。 Keep the temperature stable at 25 ± 2. C度左右,目的在于因幼苗比较弱,温度差不要过大;如果温度传感器测得环境温度为15°C,低于设定25。 About degrees C, due to weak seedlings object, the temperature difference is not too large; if the temperature sensor measures an ambient temperature of 15 ° C, below the set 25. C目标值,单片机发出信号启动电子加热片,提高环境温度;如果测得环境温度为26°C,高于目 C target, the microcontroller sends a signal to start sheet electron heating, ambient temperature increase; if the measured ambient temperature is 26 ° C, higher than the head

标设定值,单片机发出信号启动制冷片,降低环境温度。 Standard set value, the microcontroller signals the start cooling sheet, reduce the ambient temperature. 提高或降低温度直到设定值25"C; Increase or decrease the temperature set value until 25 "C;

前期培养要求保持较大湿度,减少干旱胁迫;湿度40WRH;如果湿度传感器测得环境湿度为30%RH,低于设置40%RH,单片机发出信号启动加湿 Pre-culture requires more humidity, reduced drought stress; humidity 40WRH; if the humidity sensor measured environmental humidity is 30% RH, set below 40% RH, the microcontroller sends a signal to start the humidifying

器同时发光电极发光,提高环境湿度;如果湿度传感器测定环境湿度为 Simultaneously emitting electrode to emit light, humidity increase; if the humidity sensor for measuring humidity of the environment

45%RH,高于设定值40。 45% RH, 40 is higher than the set value. /。 /. RH,单片机发出信号关闭加湿器,利用通风减少空气湿度,提高或降低湿度直到设定值40。 RH, the microcontroller sends a signal to close the humidifier using the ventilation air humidity reduction, increase or decrease until the humidity set point 40. /。 /. RH; RH;

C02气体浓度为700ppm;当C02浓度传感器测到环境中的C02浓度为500ppm,低于700ppm,单片机发出信号开通电磁阀,把C02混合气体通入到培养箱l;当C02浓度为800ppm高于预定值时,单片机发出信号关闭电磁阀,利用排风口通风减少其中的C02浓度,提高或减少其中C02浓度直到与预先设定值一致; C02 gas at a concentration of 700 ppm; C02 concentration sensor when the concentration of C02 in the environment is measured 500 ppm, less than 700 ppm, the microcontroller sends a signal to open the solenoid valve, the gas mixture passed into the C02 incubator L; C02 when the above predetermined concentration of 800ppm value, the microcontroller sends a signal to close the solenoid valve, the use of ventilation air vents reduce the concentration of C02 and C02 increased or reduced until it coincides with the concentration of the pre-set value;

第一循环参数设定较短的光照,光照时间10h,暗时间14h启动时按照预先设置程序启动,先暗培养,单片机循环检测,如果暗培养3h,低于设置14h, 继续暗培养,直到与设定值14h相一致时,单片机发出信号启动光培养,光培养时间为10h,当单片机检测到光培养时间达到预定值10h时,则循环进入至lj下一次的暗培养. The first parameter set shorter illumination cycle, the illumination time 10H, 14h dark starting time in accordance with a preset program is started, first dark culture, microcontroller loop detection, if dark culture 3h, 14h is provided below, dark culture continued until the 14h coincides set value, the microcontroller sends a signal activating the light culture, 10H light culture time, when the microcontroller detects light 10H incubation time reaches a predetermined value, the loop goes to the next lj dark culture time.

按植物生长所,需设置营养液的液位保持时间和液位高度,前期培养要求较短浸没时间;手动设置水位传感器高度为5cm;高水位时间lh、低水位时间6h;低水位时间是培养容器内尚无营养液时间,高水位时间是利用水泵上水及水位控制时间。 Press plant growth, nutrient liquid level to be set and the retention time the liquid level, the pre-culture requires shorter immersion time; manually set the height of the water level sensor 5cm; LH time high water, low water 6H time; low water level is culture time there is no time in the nutrient solution container, time is the use of high water pumps Sheung Shui and water level control time. 低水位时间结東后,开始上液,在高水位时间内,当营养液上升到设定高度5cm,水位传感器会把水位信息传递给单片机,单片机发出信号通过控制电源,停止水泵工作,并保持一段时间,直到高水位时间结東,Ul发出控制信号打开营养液回液电磁阀D3,把营养液回流到营养液存储容器3内。 The low-water East knot time, the solution began, the time in the high level, when the nutrient solution rises to the set height 5cm, water level sensor will pass information to the microcontroller, the microcontroller sends a signal through the power control, the pump stops working, and maintained period of time, until the time of high water East junction, Ul sends control signals to open solenoid valve back to the liquid nutrient solution D3, the nutrient solution back into the storage container 3 nutrient solution.

经比较循环进行时间长度和预定运行时间长度,如果小于等于5天,则继续执行第l循环;如果大于5天,则结東本循环,开始调用EEROM内的第2循环控制参数信息,开始执行第2循环内的控制参数信息,直到第2循环内的进行时间长度满足预定运行时间长度;则开始调用EEROM内的第3 循环控制参数信息;依次循环直到接收到结東信息,培养箱结束循环工作, 培养成功完成,结東整个培养过程。 By comparison cycle duration and a predetermined run length, is less than or equal to 5 days, proceed to the l cycles; if more than five days, the junction East of the cycle, starts calling the second cycle in the EEROM control parameter information, started control parameter information in the second cycle, until the length of time in the second run cycle satisfies the predetermined length of time; start calling the third cycle in the EEROM control parameter information; junction sequence loop until receiving information East, the incubator end of the cycle job training is successfully completed, the entire cultivation process knot east.

在上述循环培养过程中可根据需求通过控制面板内的选择参数SA按钮. 移动键,选择修改位置SB按钮、增加键Sc按钮.降低键、运行键So按钮.复位 In the culture process may be cyclic. Movement key, by selecting a parameter selected according to demand SA modify button in the control panel button position SB, Sc increase key button down key, the key operating button So Reset

键SE按钮的按钮时时修改当时循环的控制参数,修改后该循环进行时间长度 The buttons key SE modified from time to time when the control loop parameters, the cycle length of time the modified

为该循环的初始时间长度,预定运行时间长度不变;温度、湿度、C02气体浓度、高水位时间,低水位时间;按运行键So开始运行,执行新的控制信息; The length of time for the initial cycle, a predetermined length of time to run the same; temperature, humidity, C02 gas concentration, time of high water, low water time; So key press operation start running, performing new control information;

总之,根据微环境参数设定,自动调节微环境中温湿度、C02浓度、02 浓度和光照时间,完成培养物组织培养液的间歇供应,除了总电源开关外, 其它电源开关都由控制装置控制。 In summary, the micro-environment parameter set to automatically adjust the temperature and humidity microenvironment, C02 concentration, concentration and irradiation time 02, the intermittent supply of complete tissue culture medium, in addition to the power switch, the power switch by other control means.

以往用凝胶固体培养基组培诱导生成的根,是气生根,因为凝胶固体培养基中含有非气体相,因而在驯化过程中,原有的根在驯化基质中不起作用, 只有重新长出新鲜根才能驯化成活。 Conventional solid gel inducing tissue culture medium produced roots, root is air, because the gel-solid medium containing non-gaseous phase, and thus in the process of domestication, does not work in the original root domesticated matrix, only to re fresh roots can grow acclimated to survive. 而本实用新型用于液体间歇培养,诱导生成的根,可以直接栽到温室或大棚地里,简化植株驯化程序,降低成本。 And the present invention for liquid batch culture, induced to root, planted to a greenhouse or shed directly ground, plant acclimation to simplify procedures and reduce costs.

而且培养系统保持良好的通风换气,因而所培养的植株抗逆性显著增强,提高了组培苗驯化成活率,从而有效降低了生产成本,并且具备现有技术中气升式培养箱的所有优点。 All culture system and maintain good ventilation, so the cultured plant resistance significantly enhanced, tissue culture acclimation improved survival rate, thus effectively reducing the production cost, the prior art and have airlift incubator advantage.

从上述说明可以看出,本实用新型的实质是提供一种设计合理,同时又能实现液体培养、微环境可调、半自动化培养的装置,因此,本实用新型所说的部件不局限于上述具体形状和结构。 As can be seen from the above description, the essence of the present invention is to provide a rational design, while achieving liquid culture microenvironment adjustable, semi-automatic culture apparatus, therefore, the present invention is not limited to the above-mentioned member specific shapes and structures.

Claims (5)

1.一种间歇浸没式植物培养箱,其特征在于:包括培养箱(1),为植物组织液体培养的主要装置; 灯箱(12),内部具有照明灯,设于培养箱(1)的上部,对培养箱(1)内部的照明,提供植物所需的光照; 营养液存储容器(3)与培养箱(1)连接,为培养箱(1)提供培养植物所需的营养液。 A batch plant incubator submerged, characterized by: comprising an incubator (1) as a main unit a liquid plant tissue culture; boxes (12), having interior illumination lamp, provided in an incubator (1) of the upper portion of the incubator (1) interior lighting, to provide the required illumination plant; nutrient solution storage container (3) and the incubator (1) is connected to the incubator (1) providing a desired plant nutrient solution culture.
2. 按权利要求1所述的一种间歇浸没式植物培养箱,其特征在于:所述培养箱(l)包括工作室(2)、调控仓(11)及控制装置(6),工作室(2)内部具有液体培养容器(23 )、供气管(19),还设有温/湿度传感器(WDC/SDC), 水位传感器(SWC)、以及气体浓度传感器(QNC);供气管(19)通过进气管(18)接至调控仓(11),液体培养容器(23)与营养液存储容器(3)之间设有上液总管路(25)及回液总管路(28),上液总管路(25)上设有逆水阀(24)和水泵(M2),回液总管路(28)上设有回液电磁阀(D3);上述各传感器与控制装置(6)相连。 2. a batch according to claim submerged plant incubator of claim 1, wherein: said incubator (l) comprises a chamber (2), the regulation chamber (11) and a control device (6), studio (2) a liquid culture vessel interior (23), air supply pipe (19), also a temperature / humidity sensor (WDC / SDC), a water level sensor (the SWC), and a gas concentration sensor (QNC); supply pipe (19) connected through an intake duct (18) to the regulation of the cartridge (11), the liquid culture vessel (23) with a nutrient solution to the liquid storage container is provided with manifold passage (25) and the liquid return manifold passage (28) (3), the liquid passage is provided on the manifold (25) against the current valve (24) and pump (M2), the liquid return passage is provided with the liquid return manifold solenoid valve (D3) (28); each of the sensors connected to the control device (6).
3. 按权利要求2所述的一种间歇浸没式植物培养箱,其特征在于:所述调控仓(11 )内具有加湿器水箱(23 ),该加湿器水箱(23 )底部设有风道(13 ), 风道(13)外壁上安装有加热/制冷装置;所述风道(13)的进风口通过风机(Ml)引入空气,出风口(16)经过一电磁阀连接至工作室(2)的供气管(19) 中。 3. a batch according to claim submerged plant incubator of claim 2, wherein: said cartridge having a humidifier regulation tank (23) (11), the humidifier water tank (23) is provided with a bottom duct (13), the duct (13) is attached to the outer wall of the heating / cooling means; said duct (13) is introduced into the air inlet through the fan (of Ml), an outlet (16) connected to the working chamber via a solenoid valve ( 2) a feed pipe () 19.
4. 按权利要求3所述的一种间歇浸没式植物培养箱,其特征在于:所述电磁阀为四通电磁阀(Dl),其第一进气口(171)与风道(15)的出风口(16) 相连,第一出气口(172)通过进气管(18)接至工作室(2)的供气管(19); 第二出气口(173)接回至加湿器(10)水箱中,四通电磁阀(Dl)的第二进气口(174)与加湿器(10)的水箱相连通。 4. a batch according to claim submerged plant incubator of claim 3, wherein: said solenoid valve is a four-way solenoid valve (Dl), a first intake port (171) to a duct (15) the air outlet (16) is connected to a first outlet (172) connected to the supply pipe (19) chamber (2) through an intake pipe (18); a second outlet (173) connected back to the humidifier (10) tanks containing the four-way solenoid valve (Dl) of the second intake port (174) and a humidifier (10) in communication.
5. 按权利要求2所述的一种间歇浸没式植物培养箱,其特征在于:加湿器(10)水箱内壁上具有光触媒涂层,加湿器(10)水箱内具有发光电极。 5. a batch according to claim submerged plant incubator of claim 2, wherein: the humidifier (10) having a photocatalyst layer, the humidifier (10) having a light emitting electrode on the tank inner wall of the tank.
CNU2007200120545U 2007-05-11 2007-05-11 Intermittent immersion type plant incubators CN201015341Y (en)

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CN101300958B (en) 2007-05-11 2012-01-11 中国科学院沈阳应用生态研究所 Intermittent immersed plant cultivation device and control method thereof
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CN101300958B (en) 2007-05-11 2012-01-11 中国科学院沈阳应用生态研究所 Intermittent immersed plant cultivation device and control method thereof
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