CN106614408B - Indoor raising method for bean aphids - Google Patents
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- CN106614408B CN106614408B CN201710006222.8A CN201710006222A CN106614408B CN 106614408 B CN106614408 B CN 106614408B CN 201710006222 A CN201710006222 A CN 201710006222A CN 106614408 B CN106614408 B CN 106614408B
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- 241001425390 Aphis fabae Species 0.000 title claims abstract description 70
- 238000000034 method Methods 0.000 title claims abstract description 33
- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 74
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims abstract description 64
- 238000002791 soaking Methods 0.000 claims abstract description 20
- 240000001417 Vigna umbellata Species 0.000 claims abstract description 17
- 235000011453 Vigna umbellata Nutrition 0.000 claims abstract description 17
- 239000004744 fabric Substances 0.000 claims abstract description 8
- 239000000835 fiber Substances 0.000 claims abstract description 8
- 238000011081 inoculation Methods 0.000 claims abstract description 7
- 238000002360 preparation method Methods 0.000 claims abstract description 3
- 239000004033 plastic Substances 0.000 claims description 24
- 241001124076 Aphididae Species 0.000 claims description 22
- 239000000463 material Substances 0.000 claims description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 229910001220 stainless steel Inorganic materials 0.000 claims description 9
- 239000010935 stainless steel Substances 0.000 claims description 9
- 238000005520 cutting process Methods 0.000 claims description 8
- 238000003892 spreading Methods 0.000 claims description 8
- 239000008223 sterile water Substances 0.000 claims description 8
- 238000005303 weighing Methods 0.000 claims description 8
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims description 6
- 235000017491 Bambusa tulda Nutrition 0.000 claims description 6
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims description 6
- 239000011425 bamboo Substances 0.000 claims description 6
- 241000196324 Embryophyta Species 0.000 claims description 5
- 230000001902 propagating effect Effects 0.000 claims description 5
- 229930191978 Gibberellin Natural products 0.000 claims description 4
- 230000003203 everyday effect Effects 0.000 claims description 4
- 238000002474 experimental method Methods 0.000 claims description 4
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 claims description 4
- 239000003448 gibberellin Substances 0.000 claims description 4
- 239000002985 plastic film Substances 0.000 claims description 4
- 229920006255 plastic film Polymers 0.000 claims description 4
- 238000003825 pressing Methods 0.000 claims description 4
- 238000007789 sealing Methods 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- 238000005406 washing Methods 0.000 claims description 3
- 239000003814 drug Substances 0.000 claims description 2
- 241000721621 Myzus persicae Species 0.000 claims 3
- 244000082204 Phyllostachys viridis Species 0.000 claims 1
- 238000005286 illumination Methods 0.000 abstract description 2
- 239000000758 substrate Substances 0.000 abstract description 2
- 230000001133 acceleration Effects 0.000 abstract 1
- 230000035784 germination Effects 0.000 abstract 1
- 210000000582 semen Anatomy 0.000 description 12
- 235000010749 Vicia faba Nutrition 0.000 description 11
- 240000006677 Vicia faba Species 0.000 description 11
- 235000002098 Vicia faba var. major Nutrition 0.000 description 11
- 238000009395 breeding Methods 0.000 description 8
- 230000001488 breeding effect Effects 0.000 description 6
- 241001330002 Bambuseae Species 0.000 description 5
- 230000008901 benefit Effects 0.000 description 3
- 241001600407 Aphis <genus> Species 0.000 description 2
- 244000061176 Nicotiana tabacum Species 0.000 description 2
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 241001305034 Aphidius gifuensis Species 0.000 description 1
- 235000017060 Arachis glabrata Nutrition 0.000 description 1
- 244000105624 Arachis hypogaea Species 0.000 description 1
- 235000010777 Arachis hypogaea Nutrition 0.000 description 1
- 235000018262 Arachis monticola Nutrition 0.000 description 1
- 229930192334 Auxin Natural products 0.000 description 1
- 241001465828 Cecidomyiidae Species 0.000 description 1
- 241000255749 Coccinellidae Species 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 241000199694 Harmonia axyridis Species 0.000 description 1
- 240000004658 Medicago sativa Species 0.000 description 1
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 239000002363 auxin Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Soil Sciences (AREA)
- Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses an indoor propagation method of bean aphids, which comprises the steps of red bean seedling culture and bean aphid propagation, wherein the red bean seedling culture comprises the steps of bean seed soaking solution preparation, bean seed soaking, germination acceleration, seedling cultivation and the like, and the bean aphid propagation comprises the steps of bean aphid inoculation, seed bean aphid propagation, bean aphid propagation for propagation of natural enemies and the like. The method has low requirements on temperature, humidity and illumination, and the fiber cloth is used for keeping the humidity of the seedling substrate during seedling culture.
Description
Technical Field
The invention belongs to the technical field of biological control of agricultural and forestry pests, and relates to a propagation method of natural enemy hosts, in particular to an indoor feeding method of bean aphids.
Background
The key problem to be solved in the large-scale production of natural enemies of aphids such as ladybug, aphid eating fly and the like is the indoor propagation technology of the natural enemies of the aphids. Bean aphidAphis craccivoraKoch also called alfalfa aphid and peanut aphid, is an aphid with fast growth and reproduction and wide temperature adaptability, and is a host which is the widest used by the natural enemy of the aphid at present, such as: broad bean seedling is used for breeding aphid eating gall midge by feeding bean aphidAphidoletes sp(Beijing academy of agriculture and forestry), breeding of harmonia axyridis by broad bean aphids (Hubei province tobacco company), breeding of aphidius gifuensis (Yunnan province tobacco company) by broad bean seedlings, and the like, and the methods obtain national patents. As known from the above patent, broad bean is the most common host for breeding bean aphids. However, broad beans are large in size and not easy to be cultivated in a soilless manner, a large amount of seedling raising substrates are needed for bean seedling breeding, the period from broad bean seed to bean aphid growth to natural enemy utilization is long, and is about 30 days, and the management cost of seedling raising and bean seedling is invisibly increased. In addition, the broad bean planting area is mainly in the north and is not easily available in the south, and the technology is limited in the south. Therefore, there is a need to select a method for breeding bean aphids, which is simpler, has a wider application range and is lower in cost.
Disclosure of Invention
The invention aims to overcome the defects of the existing bean aphid breeding method, and provides an indoor bean aphid breeding method which is simpler and more convenient to operate, wider in application range and lower in cost.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows:
an indoor raising method of bean aphids comprises the following specific operation steps:
(1) cultivation of bean seedlings
① the bean seed soaking solution is prepared by weighing appropriate amount of sterile water, weighing a certain amount of the medicinal preparation A, and stirring completely in sterile water;
② soaking bean seeds, namely putting the bean seeds into a plastic basin after the bean seeds are purchased in the market, spreading, pouring a soaking solution, wherein the water level is 2cm higher than the bean seeds, and the soaking time is 12 hours;
③ pregermination of bean species comprises taking out the soaked bean species, draining, placing into plastic film bag together with plastic basin containing bean species, sealing, pregermination time is 2-3 days, taking out until the bean species grow about 3mm root, washing the soaking solution with plastic bottle, and bottling;
④ Bean seedling cultivation comprises placing seedling material soaked with water at the bottom of seedling container 1, cutting the seedling material according to the shape and size of the container bottom, spreading bean seeds on the seedling material without overlapping the individual bean seeds, placing the seedling containers in an overlapping manner, pressing a weight of about 500g on the topmost seedling container, adjusting the vertical sequence of the containers every day until a 0.5cm tender bud grows out, removing the weight, placing the seedling containers on an experiment table in a horizontal arrangement, inoculating bean aphids when the tender buds grow to 3 + -0.5 cm, cultivating for 10 days, and then using seedling container 2 to cultivate seedlings according to the method of the seedling container 1, wherein the number of seedlings grown by the seedling container 2 is 5 times of that of the container 1.
(2) Inoculation of Aphis fabae
Collecting Aphis fabae on leguminous plants, returning the Aphis fabae to the room, transferring the Aphis fabae into a seedling culture container 1 with tender shoots of 3 +/-0.5 cm, and placing the Aphis fabae on a seedling culture material;
(3) propagation of seed bean aphids
When bean seedlings in a seedling culture container 1 grow bean aphids, cutting the bean seedlings into small sections, wherein each section has 50 +/-5 aphids, transferring the bean seedling sections with the aphids into a seedling culture container 1 with newly-cultured tender shoots 3 +/-0.5 cm long, and transferring 500 +/-5 bean aphids into each seedling culture container 1;
(4) aphis fabae propagation expanding method for propagating natural enemies
And (3) when the bean seedlings in the seedling culture container 1 grow bean aphids in the expanding propagation of the seed bean aphids in the step (3), putting the support in the seedling culture container 2, putting the seedling culture container 1 on the support, taking out the seedling culture container 1 and the support after 1 day, putting the seedling culture container 1 and the support in a new seedling culture container 2, repeating for 5 times, and feeding the bean aphids for the natural enemies in the seedling culture container 2.
Further, the medicament A is gibberellin, and the concentration of the prepared soak solution is 0.5 mg/L.
Furthermore, the bean seeds are small red beans.
Further, the seedling raising container 1 is a plastic pot with phi =10cm and H =10 cm; the seedling raising container 2 is a plastic pot with phi of more than or equal to 20cm and H =10 cm.
Further, the seedling raising material is bamboo fiber cloth, and the specification is W =25cm and H =25 cm.
Further, the support is a stainless steel frame with the specification of phi =9cm and H =4 cm.
The method has the advantages and positive effects that:
1. the aphid bred by the method has low requirements on temperature and humidity and illumination, and does not need to be additionally provided with a light source, so that the management cost is reduced;
2. the method selects the small red beans to replace the broad beans, because the small red beans are wider than the broad beans in planting range in China, are easier to obtain and have regular seedlings.
3. The method selects the fiber cloth as the medium for seedling culture, is suitable for the seedling culture of the small red beans belonging to the small bean type bean species, can save the use of a large amount of seedling culture medium for the seedling culture of the soybean type bean species such as broad beans, and saves the seedling culture cost;
4. the method utilizes the principle of plant seed spindling under the condition of weak light and reasonable use of auxin, thereby controlling the growth height of small red bean seedlings.
5. The small red bean used in the method can be used for inoculating aphids after being sown, only 4 days are needed, and only 6 days are needed from the inoculation of the aphids to the growth of the aphids on bean seedlings, so that the method saves more than half of the time for propagating the aphids by using the broad bean seedlings, and has better economic benefit and social benefit.
Drawings
FIG. 1: is a process flow schematic diagram of the indoor feeding method of the bean aphids.
FIG. 2: is a combined schematic diagram of the bean aphid propagation inoculation source container.
Fig. 2 identifies: 1-seedling raising container 1; 2-seedling raising container 2; 3-stainless steel frame.
Detailed Description
The process of the present invention is described in further detail below by way of examples, but it should be understood that the invention is not limited thereto.
Example 1:
an indoor raising method of bean aphids comprises the following specific operation steps:
(1) cultivation of bean seedlings
① the bean seed soaking solution is prepared by weighing appropriate amount of sterile water, weighing gibberellin A, adding into sterile water, stirring, and making into soaking solution with concentration of 0.5 mg/L;
② soaking semen Phaseoli, which is obtained by placing semen Phaseoli in plastic basin, spreading, adding soaking solution at water level 2cm higher than semen Phaseoli for 12 hr, wherein the size of the plastic basin is selected according to the number of semen Phaseoli soaked;
③ germinating bean seeds by taking out the soaked bean seeds, draining, placing into a plastic film bag together with a plastic basin containing the bean seeds, sealing, germinating for 2 days until the bean seeds grow about 3mm root, taking out, washing the soaking solution with plastic bottles, and reserving;
④ Bean seedling cultivation comprises placing bamboo fiber cloth seedling material with specification of W =25cm and H =25cm at the bottom of plastic pot seedling container 1 with specification of phi =10cm and H =10cm, cutting the seedling material according to the shape and size of the container bottom before placing, spreading semen Phaseoli bean seeds on the bamboo fiber cloth seedling material without overlapping bean seeds, placing the seedling containers in an overlapping manner, pressing a weight of about 500g on the seedling container at the top, adjusting the vertical sequence of the containers every day until a tender bud with length of 0.5cm grows out, removing the weight, placing the seedling containers on an experiment table in a horizontal arrangement, inoculating Aphis virescens when the tender bud of semen Phaseoli grows to 3 + -0.5 cm, cultivating for 10d, and using plastic pot container 2 with phi =40cm and H =10cm to cultivate the quantity of seedling container 25 times that of seedling container 1 according to the method of the container 1.
(2) Inoculation of Aphis fabae
Collecting Aphis fabae on leguminous plants, returning the Aphis fabae to the room, transferring the Aphis fabae into a seedling culture container 1 with 3 +/-0.5 cm of small red bean buds, and placing the Aphis fabae on a seedling culture material;
(3) propagation of seed bean aphids
When the bean seedlings of the small red beans in the seedling culture container 1 are full of bean aphids, cutting the bean seedlings of the small red beans into small sections, wherein each section has 50 +/-5 aphids, transferring the bean seedling sections with the aphids into the seedling culture container 1 with newly-cultured bean sprouts of 3 +/-0.5 cm, and transferring 500 +/-5 bean aphids into each seedling culture container 1;
(4) aphis fabae propagation expanding method for propagating natural enemies
And (3) when the bean aphids grow on the small red bean seedlings in the seedling culture container 1 in the expanding propagation of the bean aphids of provenance in the step (3), putting a stainless steel bracket with the specification of phi =9cm and H =4cm into the seedling culture container 2, putting the seedling culture container 1 on the bracket, taking out the seedling culture container 1 and the stainless steel bracket after 1 day, putting the seedling culture container 1 and the stainless steel bracket into a new seedling culture container 2, repeating for 5 times, and feeding the bean aphids for natural enemies in the seedling culture container 2.
Example 2:
an indoor raising method of bean aphids comprises the following specific operation steps:
(1) cultivation of bean seedlings
① the bean seed soaking solution is prepared by weighing appropriate amount of sterile water, weighing gibberellin A, adding into sterile water, stirring, and making into soaking solution with concentration of 0.5 mg/L;
② soaking semen Phaseoli, which is obtained by placing semen Phaseoli in plastic basin, spreading, adding soaking solution at water level 2cm higher than semen Phaseoli for 12 hr, wherein the size of the plastic basin is selected according to the number of semen Phaseoli soaked;
③ germinating bean seeds by taking out the soaked bean seeds, draining, putting into a plastic film bag together with a plastic basin containing the bean seeds, sealing, germinating for 3 days until the bean seeds grow about 3mm roots, and bottling the soaking solution with a plastic bottle;
④ Bean seedling cultivation comprises placing bamboo fiber cloth seedling material with specification of W =25cm and H =25cm at the bottom of plastic pot seedling container 1 with specification of phi =10cm and H =10cm, cutting the seedling material according to the shape and size of the container bottom before placing, spreading semen Phaseoli bean seeds on the bamboo fiber cloth seedling material without overlapping bean seeds, placing the seedling containers in an overlapping manner, pressing a weight of about 500g on the seedling container at the top, adjusting the vertical sequence of the containers every day until a tender bud with length of 0.5cm grows out, removing the weight, placing the seedling containers on an experiment table in a horizontal arrangement, inoculating Aphis virescens when the tender bud of semen Phaseoli grows to 3 + -0.2 cm, cultivating for 10d, and using plastic pot container 2 with phi =30cm and H =10cm, and cultivating the number of seedling container 2 is 3 times of that of seedling container 1 according to the method of the above container 1.
(2) Inoculation of Aphis fabae
Collecting Aphis fabae on leguminous plants, returning the Aphis fabae to the room, transferring the Aphis fabae into a seedling culture container 1 with 3 +/-0.2 cm of small red bean buds, and placing the Aphis fabae on a seedling culture material;
(3) propagation of seed bean aphids
When the bean seedlings of the small red beans in the seedling culture container 1 are full of bean aphids, cutting the bean seedlings of the small red beans into small sections, wherein 50 +/-5 aphids exist in each section, transferring the bean seedling sections with the aphids into the seedling culture container 1 with newly-cultured bean sprouts of 3 +/-0.2 cm in length, and transferring 500 +/-2 bean aphids into each seedling culture container 1;
(4) aphis fabae propagation expanding method for propagating natural enemies
And (3) when the bean aphids grow on the small red bean seedlings in the seedling culture container 1 in the expanding propagation of the bean aphids of provenance in the step (3), putting a stainless steel bracket with the specification of phi =9cm and H =4cm into the seedling culture container 2, putting the seedling culture container 1 on the bracket, taking out the seedling culture container 1 and the stainless steel bracket after 1 day, putting the seedling culture container 1 and the stainless steel bracket into a new seedling culture container 2, repeating for 5 times, and feeding the bean aphids for natural enemies in the seedling culture container 2.
Claims (4)
1. An indoor raising method of bean aphids is characterized by comprising the following steps: the specific operation steps are as follows:
(1) cultivation of bean seedlings
① the bean seed soaking solution is prepared by weighing appropriate amount of sterile water, weighing a certain amount of the medicinal preparation A, and stirring completely in sterile water;
③ pregermination of bean species comprises taking out the soaked bean species, draining, placing into plastic film bag together with plastic basin containing bean species, sealing, pregermination time is 2-3 days, taking out until the bean species grow about 3mm root, washing the soaking solution with plastic bottle, and bottling;
④ cultivation of bean seedlings, namely putting seedling cultivation materials soaked with water at the bottom of a first seedling cultivation container (1), cutting the seedling cultivation materials according to the shape and size of the bottom of the first seedling cultivation container (1) before putting, then spreading bean seeds on the seedling cultivation materials, wherein the bean seeds are not overlapped, after spreading, overlapping the first seedling cultivation container (1), pressing a weight of 500g on the first seedling cultivation container (1) at the top, adjusting the vertical sequence of the first seedling cultivation container (1) every day until a tender bud with the length of 0.5cm grows out, removing the weight, horizontally arranging the first seedling cultivation container (1) on an experiment table, inoculating bean aphids when the tender buds grow to 3 +/-0.5 cm, after cultivating for 10d, cultivating the second seedling cultivation container (2) by the method of the first seedling cultivation container (1), and the quantity of the second seedling cultivation container (2) is 5 times of that of the first seedling cultivation container (1);
(2) inoculation of Aphis fabae
Collecting Aphis fabae on leguminous plants, returning the Aphis fabae to the room, transferring the Aphis fabae into a first seedling culture container (1) with tender shoots of 3 +/-0.5 cm, and placing the Aphis fabae on a seedling culture material;
(3) propagation of seed bean aphids
When bean seedlings in the first seedling raising container (1) are full of bean aphids, cutting the bean seedlings into small sections, wherein each section has 50 +/-5 aphids, transferring the bean seedling sections with the aphids into the first seedling raising containers (1) with newly-cultured tender shoots of 3 +/-0.5 cm in length, and transferring 500 +/-5 bean aphids into each first seedling raising container (1);
(4) aphis fabae propagation expanding method for propagating natural enemies
When bean aphids grow on bean seedlings in the first seedling raising container (1) in the expanding propagation of the bean aphids of provenance in the step (3), putting a support in the second seedling raising container (2), putting the first seedling raising container (1) on the support, taking out the first seedling raising container (1) and the support after 1 day, putting the first seedling raising container and the support in the new second seedling raising container (2), repeating for 5 times, and feeding the bean aphids for natural enemies in the second seedling raising container (2);
the medicament A is gibberellin, and the concentration of the prepared soak solution is 0.5 mg/L;
the bean is small red bean.
2. The indoor feeding method of myzus persicae as claimed in claim 1, wherein: the first seedling raising container (1) is a plastic pot with the diameter =10cm and the height =10 cm; the second seedling raising container (2) is a plastic pot with the diameter being more than or equal to 20cm and the height being =10 cm.
3. The indoor feeding method of myzus persicae as claimed in claim 1, wherein: the seedling raising material is bamboo fiber cloth, and the specification is that the length =25cm, and the width =25 cm.
4. The indoor feeding method of myzus persicae as claimed in claim 1, wherein: the support be stainless steel frame (3), the specification is diameter =9cm, and the height =4 cm.
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| CN109247303A (en) * | 2018-11-16 | 2019-01-22 | 湖北省农业科学院粮食作物研究所 | Utilize the method for water planting semen viciae fabae seedling mass propagation bean aphid |
Citations (3)
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|---|---|---|---|---|
| CN101601375A (en) * | 2008-06-12 | 2009-12-16 | 中国农业科学院植物保护研究所 | A kind of artificial fecundation method of aphid |
| CN102301982A (en) * | 2011-07-19 | 2012-01-04 | 云南省烟草公司曲靖市公司 | Method for propagating Aphidius gifuensis by pea aphids |
| CN103155906A (en) * | 2013-04-02 | 2013-06-19 | 北京市农林科学院 | Aphidoletes aphidimyza mass reproduction technology and application technology |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US3786594A (en) * | 1972-04-17 | 1974-01-22 | Beneficial Insect Control Inc | Method of raising and handling wasps |
| ES2376097B1 (en) * | 2010-03-26 | 2013-02-28 | Universidad De Alicante | BREEDING METHOD OF SPHAEROPHORIA RUEPPELLII PREDATOR. |
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|---|---|---|---|---|
| CN101601375A (en) * | 2008-06-12 | 2009-12-16 | 中国农业科学院植物保护研究所 | A kind of artificial fecundation method of aphid |
| CN102301982A (en) * | 2011-07-19 | 2012-01-04 | 云南省烟草公司曲靖市公司 | Method for propagating Aphidius gifuensis by pea aphids |
| CN103155906A (en) * | 2013-04-02 | 2013-06-19 | 北京市农林科学院 | Aphidoletes aphidimyza mass reproduction technology and application technology |
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| Title |
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| 豌豆蚜天敌——阿尔蚜茧蜂繁殖与利用研究;何琬等;《植物保护学报》;19830930;第10卷(第03期);全文 * |
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