CN106614408B - Indoor raising method for bean aphids - Google Patents

Indoor raising method for bean aphids Download PDF

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CN106614408B
CN106614408B CN201710006222.8A CN201710006222A CN106614408B CN 106614408 B CN106614408 B CN 106614408B CN 201710006222 A CN201710006222 A CN 201710006222A CN 106614408 B CN106614408 B CN 106614408B
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aphids
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CN106614408A (en
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邓艳
蒋学建
黄华艳
常明山
罗辑
赵程劼
邹东霞
廖旺姣
秦元丽
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Hunan Academy of Forestry
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/30Rearing or breeding invertebrates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics

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Abstract

本发明公开了一种豆蚜的室内繁殖方法,该方法包括红小豆的育苗和豆蚜的扩繁,红小豆的育苗包括豆种浸泡液的配制、豆种的浸泡、催芽、育苗等步骤,豆蚜的扩繁包括豆蚜的接种、种源豆蚜的扩繁、用于繁殖天敌的豆蚜扩繁等步骤。本发明使用的方法,对温湿度和光照的要求不高,育苗时使用纤维布保持育苗基质的湿度。

Figure 201710006222

The invention discloses an indoor propagation method of bean aphid, which comprises the steps of raising adzuki bean seedlings and propagating the bean aphid. The propagation of bean aphid includes steps such as inoculation of bean aphid, propagation of provenance bean aphid, and propagation of bean aphid used to breed natural enemies. The method used in the present invention does not have high requirements on temperature, humidity and light, and fiber cloth is used to maintain the humidity of the seedling substrate during seedling raising.

Figure 201710006222

Description

一种豆蚜的室内饲养方法A kind of indoor raising method of bean aphid

技术领域technical field

本发明属于农林害虫生物防治技术领域,涉及一种天敌寄主的繁殖方法,尤其是一种豆蚜的室内饲养方法。The invention belongs to the technical field of biological control of agricultural and forestry pests, and relates to a breeding method for a natural enemy host, in particular to an indoor breeding method for bean aphid.

背景技术Background technique

瓢虫、食蚜蝇等蚜虫天敌的规模化生产首先要解决的关键问题就是天敌蚜虫的室内繁殖技术。豆蚜Aphis craccivora Koch又名苜蓿蚜、花生蚜,是一种生长繁殖快,温度适应性广的蚜虫,是目前蚜虫天敌繁殖使用最广的一种寄主,如:用蚕豆苗饲养豆蚜繁殖食蚜瘿蚊Aphidoletes sp(北京市农林科学院)、用蚕豆饲养豆蚜繁殖异色瓢虫(湖北省烟草公司)、用蚕豆苗饲养豆蚜繁殖呀烟蚜茧蜂(云南省烟草公司)等,以上方法都获得了国家专利。由上述专利可知,蚕豆是繁殖豆蚜最常用的一种寄主。但是,蚕豆个体大,不易于无土栽培,繁殖豆苗需要大量的育苗基质,且从蚕豆种到豆蚜生长到能为天敌利用的周期长,达30天左右,这无形中增加了育苗和豆苗的管理成本。另外,蚕豆的种植区主要在北方,在南方不容易获得,此类技术在南方受到诸多限制。因此,有必要筛选出一种更简便,适应范围更广以及成本更低的豆蚜繁殖方法。The first key problem to be solved in the large-scale production of natural enemies of aphids such as ladybugs and hoverflies is the indoor breeding technology of natural enemies aphids. The bean aphid Aphis craccivora Koch, also known as alfalfa aphid and peanut aphid, is a kind of aphid with fast growth and reproduction and wide temperature adaptability. Aphidoletes sp (Beijing Academy of Agriculture and Forestry), Raising bean aphids with broad bean to breed Axychromis axyridis (Hubei Tobacco Company), breeding bean aphids with broad bean seedlings for breeding Aphididae (Yunnan Tobacco Company), etc., above The methods have obtained national patents. As can be seen from the above patent, broad bean is the most commonly used host for breeding bean aphid. However, broad beans are large in size and difficult to cultivate without soil. Propagation of bean seedlings requires a large amount of seedling substrate, and the period from broad bean seed to bean aphid growth to being used by natural enemies is about 30 days. Management costs of bean sprouts. In addition, the planting area of broad bean is mainly in the north, and it is not easy to obtain in the south, and such technologies are subject to many restrictions in the south. Therefore, it is necessary to screen out a simpler, more suitable and lower cost bean aphid propagation method.

发明内容SUMMARY OF THE INVENTION

本发明的目的在于克服现阶段豆蚜繁殖方法中存在的不足,提供一种操作更简便、适应范围更广以及成本更低的豆蚜室内繁殖方法。The purpose of the present invention is to overcome the deficiencies existing in the current bean aphid propagation method, and to provide a bean aphid indoor propagation method with simpler operation, wider adaptability and lower cost.

为了实现以上目的,本发明采用的技术方案如下:In order to achieve the above purpose, the technical scheme adopted in the present invention is as follows:

一种豆蚜的室内饲养方法,具体操作步骤是:A kind of indoor raising method of bean aphid, the concrete operation steps are:

(1)豆苗的培育(1) Cultivation of bean sprouts

①豆种浸泡液的配制:量取适量的无菌水,称取一定量药剂A,倒入无菌水中搅拌均匀; ①Preparation of bean seed soaking solution: take an appropriate amount of sterile water, weigh a certain amount of drug A, pour it into the sterile water and stir evenly;

②豆种的浸泡:市场上购得豆种后,将豆种放入塑料盆中,摊平,倒入浸泡液,水位高于豆种2cm,浸泡时间为12h;所述的塑料盆的大小,根据浸泡的豆种数量选取;②Soaking of bean seeds: After purchasing the bean seeds in the market, put the bean seeds into a plastic pot, flatten them, pour the soaking liquid, the water level is 2cm higher than the bean seeds, and the soaking time is 12h; the size of the plastic pot is , according to the number of soaked beans;

③豆种的催芽:将浸泡好的豆种捞出,沥干水分,连同盛豆种的塑料盆一同放入塑料薄膜袋中,封口,催芽时间为2-3天,直至豆种长出3mm左右的根时取出;浸泡液用塑料洗瓶装好备用;③ Germination of bean seeds: Take out the soaked bean seeds, drain the water, put them into a plastic film bag together with the plastic pot containing the bean seeds, seal, and the germination time is 2-3 days, until the bean seeds grow 3mm Take out the left and right roots; the soaking solution is packed in a plastic washing bottle for later use;

④豆苗的培育:在育苗容器1的底部放入浸透水分的育苗材料,放入前将育苗材料根据容器底部的形状和大小进行裁剪,再将豆种铺满在育苗材料上,豆种个体之间不重叠;铺好后,将育苗容器重叠放置,在最顶部的育苗容器上压上一个500g左右的重物,每天调整容器的上下顺序,直至有0.5cm长的嫩芽长出时,移除重物,将育苗容器平排放置在实验台上;当嫩芽长到3±0.5cm时即可接种豆蚜;培育10d后,用育苗容器2,按上述育苗容器1的方法育苗,育苗容器2育苗的数量为容器1的5倍。4. Cultivation of bean sprouts: put the seedling material soaked with water at the bottom of the seedling container 1, cut the seedling material according to the shape and size of the bottom of the container before putting in, and then spread the bean seeds on the seedling material, and the individual bean seeds There is no overlap between them; after laying, place the seedling containers on top of each other, press a weight of about 500g on the top seedling container, and adjust the up and down order of the containers every day until there are 0.5cm long shoots. Remove the heavy objects, and place the seedling container on the test bench in a horizontal row; when the sprouts grow to 3±0.5cm, the bean aphid can be inoculated; after cultivating 10d, use the seedling container 2 to raise the seedlings according to the method of the above-mentioned seedling container 1, The number of seedlings in container 2 is 5 times that of container 1.

(2)豆蚜的接种(2) Inoculation of bean aphid

在豆科植物上采集豆蚜带回室内,移入嫩芽长3±0.5cm的育苗容器1中,置于育苗材料上即可;Collect bean aphids on legumes and bring them back to the room, move them into a seedling container 1 with a sprout length of 3±0.5 cm, and place them on the seedling material;

(3)种源豆蚜的扩繁(3) Propagation of Provenance Bean Aphid

待育苗容器中1的豆苗上长满豆蚜时,将豆苗剪成小段,每段有蚜虫50±5只,将有蚜虫的豆苗段移入新培育嫩芽长3±0.5cm的育苗容器1中,每育苗容器1中移入豆蚜500±5只;When the bean sprouts of 1 in the seedling raising container are covered with bean aphids, cut the bean sprouts into small sections, each section has 50 ± 5 aphids, and move the bean sprouts section with aphids into the newly cultivated seedlings with a length of 3 ± 0.5 cm. In container 1, 500 ± 5 bean aphids are moved into each seedling container 1;

(4)用于繁殖天敌的豆蚜扩繁(4) The bean aphid used to reproduce natural enemies is propagated

待步骤(3)种源豆蚜的扩繁中育苗容器中1的豆苗上长满豆蚜时,在育苗容器2中放入支架,将育苗容器中1置于支架上,1天后取出育苗容器中1和支架置于新的育苗容器2中,重复5次,在育苗容器2饲养得到用于天敌的豆蚜。When the bean sprouts in the seedling container 1 in the propagation of the provenance bean aphid in step (3) are covered with bean aphids, put a support in the seedling container 2, place the seedling container 1 on the support, and take out the seedlings after 1 day The container 1 and the bracket were placed in a new seedling container 2, repeated 5 times, and the bean aphid for natural enemies was obtained by rearing in the seedling container 2.

进一步地,所述的药剂A为赤霉素,配制的浸泡液浓度为0.5mg/L。Further, the agent A is gibberellin, and the concentration of the prepared soaking solution is 0.5 mg/L.

进一步地,所述的豆种为红小豆。Further, the bean species is adzuki bean.

进一步地,所述的育苗容器1为Φ=10cm,H=10cm的塑料盆;所述的育苗容器2为Φ≥20cm,H=10cm的塑料盆。Further, the seedling container 1 is a plastic pot with Φ=10cm and H=10cm; the seedling container 2 is a plastic pot with Φ≥20cm and H=10cm.

进一步地,所述的育苗材料为竹纤维布,规格为W=25cm,H=25cm。Further, described seedling raising material is bamboo fiber cloth, and the specification is W=25cm, H=25cm.

进一步地,所述的支架为不锈钢架规格为Φ=9cm,H=4cm。Further, the described support is a stainless steel frame with specifications of Φ=9cm, H=4cm.

本发明方法的优点和积极效果:Advantages and positive effects of the inventive method:

1、使用本方法繁殖蚜虫,对温湿度的要求不高,对光照的要求也不高,不需要额外增加光源,降低了管理成本;1. Using this method to breed aphids does not require high temperature and humidity, nor does it require high light, so there is no need to add additional light sources, which reduces management costs;

2、本方法选用红小豆代替蚕豆,因为红小豆在我国的种植范围较蚕豆广,更容易获得,出苗整齐。2. In this method, adzuki bean is used instead of broad bean, because adzuki bean has a wider planting range than broad bean in my country, is easier to obtain, and emerges neatly.

3、本方法选用纤维布作为介质育苗,适合红小豆属于小豆型豆种育苗使用,可以省去大豆型豆种如蚕豆育苗必须使用大量育苗基质,节省了育苗成本;3. In this method, fiber cloth is used as the medium for seedling raising, which is suitable for adzuki bean, which belongs to the adzuki bean type, and can save the use of a large number of seedling substrates for the seedling raising of soybean type bean seeds such as broad bean, which saves the cost of raising seedlings;

4、本方法利用植物种子在弱光照条件下的徒长原理,加上生长素的合理使用,从而控制红小豆苗的生长高度。4. The method utilizes the principle of leggy growth of plant seeds under weak light conditions and the rational use of auxin, thereby controlling the growth height of adzuki bean sprouts.

5、本方法的使用的红小豆从播种达到可以接种蚜虫,只需要4d时间,从接种蚜虫到豆苗长满蚜虫也只需要6d,较用蚕豆苗繁殖蚜虫的时间节省了一半多,具有较好的经济效益和社会效益。5. The adzuki beans used in this method can be inoculated with aphids, which only takes 4 days, and it only takes 6 days from inoculation with aphids to when the bean sprouts are covered with aphids, which saves more than half of the time required to propagate aphids with broad bean sprouts, and has a relatively low cost. good economic and social benefits.

附图说明Description of drawings

图1:为本发明一种豆蚜的室内饲养方法工艺流程示意图。Fig. 1: is a kind of indoor breeding method schematic diagram of bean aphid of the present invention.

图2:为本发明豆蚜扩繁接种源容器组合示意图。Fig. 2 is a schematic diagram showing the combination of the inoculation source container for bean aphid propagation according to the present invention.

图2标识:1-育苗容器1;2-育苗容器2;3-不锈钢架。Figure 2 Identification: 1-seedling container 1; 2-seedling container 2; 3-stainless steel frame.

具体实施方式Detailed ways

以下通过实施例对本发明方法作进一步的详细描述,但应该理解本发明并不受这些内容所限制。The method of the present invention will be further described in detail below through the examples, but it should be understood that the present invention is not limited by these contents.

实施例1:Example 1:

一种豆蚜的室内饲养方法,具体操作步骤是:A kind of indoor raising method of bean aphid, the concrete operation steps are:

(1)豆苗的培育(1) Cultivation of bean sprouts

①豆种浸泡液的配制:量取适量的无菌水,称取药剂A赤霉素,倒入无菌水中搅拌均匀,配制成浓度为0.5mg/L的浸泡液; ①Preparation of bean seed soaking solution: Measure an appropriate amount of sterile water, weigh the drug A gibberellin, pour it into the sterile water and stir evenly to prepare a soaking solution with a concentration of 0.5mg/L;

②豆种的浸泡:市场上购得豆种红小豆后,将红小豆放入塑料盆中,摊平,倒入浸泡液,水位高于豆种2cm,浸泡时间为12h;所述的塑料盆的大小,根据浸泡的豆种数量选取;②Soaking of bean seeds: After purchasing the bean seeds in the market, put the azuki beans in a plastic basin, flatten them, pour the soaking liquid, the water level is 2cm higher than the bean seeds, and the soaking time is 12h; the plastic basin The size is selected according to the number of soaked beans;

③豆种的催芽:将浸泡好的红小豆豆种捞出,沥干水分,连同盛红小豆豆种的塑料盆一同放入塑料薄膜袋中,封口,催芽时间为2天,直至豆种红小豆长出3mm左右的根时取出;浸泡液用塑料洗瓶装好备用;③ Germination of bean seeds: Take out the soaked adzuki bean seeds, drain the water, put them into a plastic film bag together with the plastic pot containing the adzuki bean seeds, seal, and the germination time is 2 days, until the bean seeds are red. Take out the adzuki beans when they grow a root of about 3mm; the soaking liquid is packed in a plastic washing bottle for later use;

④豆苗的培育:在Φ=10cm,H=10cm的塑料盆育苗容器1的底部放入浸透水分,规格为W=25cm,H=25cm的竹纤维布育苗材料,放入前将育苗材料根据容器底部的形状和大小进行裁剪,再将红小豆豆种铺满在竹纤维布育苗材料上,豆种个体之间不重叠;铺好后,将育苗容器重叠放置,在最顶部的育苗容器上压上一个500g左右的重物,每天调整容器的上下顺序,直至有0.5cm长的嫩芽长出时,移除重物,将育苗容器平排放置在实验台上;当红小豆嫩芽长到3±0.5cm时即可接种豆蚜;培育10d后,用Φ=40cm,H=10cm的塑料盆育苗容器2,按上述育苗容器1的方法育苗,育苗容器2育苗的数量为容器1的5倍。④Cultivation of bean sprouts: put soaked water in the bottom of the plastic pot seedling raising container 1 of Φ=10cm, H=10cm, the specification is the bamboo fiber cloth seedling raising material of W=25cm, H=25cm, put the seedling raising material according to Cut out the shape and size of the bottom of the container, and then spread the adzuki bean seeds on the bamboo fiber cloth seedling raising material, and the individual beans do not overlap; Press a weight of about 500g, and adjust the up and down order of the container every day until 0.5cm long sprouts grow, remove the weight, and place the seedling container on the test bench; when the red bean sprouts grow to Bean aphid can be inoculated when 3±0.5cm; after cultivating 10d, use Φ=40cm, H=10cm plastic pot seedling container 2, raise seedlings according to the method of the above-mentioned seedling container 1, and the number of seedlings in the seedling container 2 is 5 of the container 1. times.

(2)豆蚜的接种(2) Inoculation of bean aphid

在豆科植物上采集豆蚜带回室内,移入红小豆嫩芽长3±0.5cm的育苗容器1中,置于育苗材料上即可;Collect bean aphids on legumes and bring them back to the room, transfer them into a seedling container 1 with a sprout length of 3±0.5 cm of adzuki bean, and place them on the seedling material;

(3)种源豆蚜的扩繁(3) Propagation of Provenance Bean Aphid

待育苗容器中1的红小豆豆苗上长满豆蚜时,将红小豆豆苗剪成小段,每段有蚜虫50±5只,将有蚜虫的红小豆豆苗段移入新培育红小豆嫩芽长3±0.5cm的育苗容器1中,每育苗容器1中移入豆蚜500±5只;When the adzuki bean sprouts of 1 in the seedling raising container are covered with bean aphids, cut the adzuki bean sprouts into small sections, each section has 50±5 aphids, and move the adzuki bean sprouts with aphids into the newly cultivated adzuki bean sprouts. In the seedling container 1 with a bud length of 3±0.5cm, 500±5 bean aphids were moved into each seedling container 1;

(4)用于繁殖天敌的豆蚜扩繁(4) The bean aphid used to reproduce natural enemies is propagated

待步骤(3)种源豆蚜的扩繁中育苗容器中1的红小豆豆苗上长满豆蚜时,在育苗容器2中放入规格为Φ=9cm,H=4cm不锈钢支架,将育苗容器中1置于支架上,1天后取出育苗容器中1和不锈钢支架置于新的育苗容器2中,重复5次,在育苗容器2饲养得到用于天敌的豆蚜。In step (3), when the adzuki bean sprouts in the seedling container 1 are covered with bean aphids in the propagation of the provenance bean aphid, put a stainless steel support with a size of Φ=9cm, H=4cm in the seedling container 2, and place the seedlings in the seedling container 2. The container 1 was placed on a support, and after 1 day, the seedling container 1 and the stainless steel support were taken out and placed in a new seedling container 2, repeated 5 times, and the bean aphid for natural enemies was obtained by rearing in the seedling container 2.

实施例2:Example 2:

一种豆蚜的室内饲养方法,具体操作步骤是:A kind of indoor raising method of bean aphid, the concrete operation steps are:

(1)豆苗的培育(1) Cultivation of bean sprouts

①豆种浸泡液的配制:量取适量的无菌水,称取药剂A赤霉素,倒入无菌水中搅拌均匀,配制成浓度为0.5mg/L的浸泡液; ①Preparation of bean seed soaking solution: Measure an appropriate amount of sterile water, weigh the drug A gibberellin, pour it into the sterile water and stir evenly to prepare a soaking solution with a concentration of 0.5mg/L;

②豆种的浸泡:市场上购得豆种红小豆后,将红小豆放入塑料盆中,摊平,倒入浸泡液,水位高于豆种2cm,浸泡时间为12h;所述的塑料盆的大小,根据浸泡的豆种数量选取;②Soaking of bean seeds: After purchasing the bean seeds in the market, put the azuki beans in a plastic basin, flatten them, pour the soaking liquid, the water level is 2cm higher than the bean seeds, and the soaking time is 12h; the plastic basin The size is selected according to the number of soaked beans;

③豆种的催芽:将浸泡好的红小豆豆种捞出,沥干水分,连同盛红小豆豆种的塑料盆一同放入塑料薄膜袋中,封口,催芽时间为3天,直至豆种红小豆长出3mm左右的根时取出;浸泡液用塑料洗瓶装好备用;③ Germination of bean seeds: Take out the soaked adzuki bean seeds, drain the water, put them into a plastic film bag together with the plastic pot containing the adzuki bean seeds, seal, and the germination time is 3 days, until the bean seeds are red. Take out the adzuki beans when they grow a root of about 3mm; the soaking liquid is packed in a plastic washing bottle for later use;

④豆苗的培育:在Φ=10cm,H=10cm的塑料盆育苗容器1的底部放入浸透水分,规格为W=25cm,H=25cm的竹纤维布育苗材料,放入前将育苗材料根据容器底部的形状和大小进行裁剪,再将红小豆豆种铺满在竹纤维布育苗材料上,豆种个体之间不重叠;铺好后,将育苗容器重叠放置,在最顶部的育苗容器上压上一个500g左右的重物,每天调整容器的上下顺序,直至有0.5cm长的嫩芽长出时,移除重物,将育苗容器平排放置在实验台上;当红小豆嫩芽长到3±0.2cm时即可接种豆蚜;培育10d后,用Φ=30cm,H=10cm的塑料盆育苗容器2,按上述育苗容器1的方法育苗,育苗容器2育苗的数量为容器1的3倍。④Cultivation of bean sprouts: put soaked water in the bottom of the plastic pot seedling raising container 1 of Φ=10cm, H=10cm, the specification is the bamboo fiber cloth seedling raising material of W=25cm, H=25cm, put the seedling raising material according to Cut out the shape and size of the bottom of the container, and then spread the adzuki bean seeds on the bamboo fiber cloth seedling raising material, and the individual beans do not overlap; Press a weight of about 500g, and adjust the up and down order of the container every day until 0.5cm long sprouts grow, remove the weight, and place the seedling container on the test bench; when the red bean sprouts grow to Bean aphid can be inoculated when 3±0.2cm; after cultivating 10d, use Φ=30cm, H=10cm plastic pot seedling container 2, raise seedlings according to the method of the above-mentioned seedling container 1, and the number of seedlings in the seedling container 2 is 3 of the container 1. times.

(2)豆蚜的接种(2) Inoculation of bean aphid

在豆科植物上采集豆蚜带回室内,移入红小豆嫩芽长3±0.2cm的育苗容器1中,置于育苗材料上即可;Bean aphids are collected from legumes and brought back indoors, transferred into a seedling container 1 with a sprout length of 3±0.2 cm of adzuki bean, and placed on the seedling material;

(3)种源豆蚜的扩繁(3) Propagation of Provenance Bean Aphid

待育苗容器中1的红小豆豆苗上长满豆蚜时,将红小豆豆苗剪成小段,每段有蚜虫50±5只,将有蚜虫的红小豆豆苗段移入新培育红小豆嫩芽长3±0.2cm的育苗容器1中,每育苗容器1中移入豆蚜500±2只;When the adzuki bean sprouts of 1 in the seedling raising container are covered with bean aphids, cut the adzuki bean sprouts into small sections, each section has 50±5 aphids, and move the adzuki bean sprouts with aphids into the newly cultivated adzuki bean sprouts. In the seedling container 1 with a bud length of 3±0.2 cm, 500±2 bean aphids were moved into each seedling container 1;

(4)用于繁殖天敌的豆蚜扩繁(4) The bean aphid used to reproduce natural enemies is propagated

待步骤(3)种源豆蚜的扩繁中育苗容器中1的红小豆豆苗上长满豆蚜时,在育苗容器2中放入规格为Φ=9cm,H=4cm不锈钢支架,将育苗容器中1置于支架上,1天后取出育苗容器中1和不锈钢支架置于新的育苗容器2中,重复5次,在育苗容器2饲养得到用于天敌的豆蚜。In step (3), when the adzuki bean sprouts in the seedling container 1 are covered with bean aphids in the propagation of the provenance bean aphid, put a stainless steel support with a size of Φ=9cm, H=4cm in the seedling container 2, and place the seedlings in the seedling container 2. The container 1 was placed on a support, and after 1 day, the seedling container 1 and the stainless steel support were taken out and placed in a new seedling container 2, repeated 5 times, and the bean aphid for natural enemies was obtained by rearing in the seedling container 2.

Claims (4)

1. An indoor raising method of bean aphids is characterized by comprising the following steps: the specific operation steps are as follows:
(1) cultivation of bean seedlings
① the bean seed soaking solution is prepared by weighing appropriate amount of sterile water, weighing a certain amount of the medicinal preparation A, and stirring completely in sterile water;
Figure 474586DEST_PATH_IMAGE001
soaking bean seeds: after the bean seeds are purchased on the market, the bean seeds are put into a plastic basin, spread out and poured into the plastic basin for soakingSoaking bean seeds in water for 12h, wherein the water level is 2cm higher than the bean seeds; the size of the plastic basin is selected according to the number of the soaked bean seeds;
③ pregermination of bean species comprises taking out the soaked bean species, draining, placing into plastic film bag together with plastic basin containing bean species, sealing, pregermination time is 2-3 days, taking out until the bean species grow about 3mm root, washing the soaking solution with plastic bottle, and bottling;
④ cultivation of bean seedlings, namely putting seedling cultivation materials soaked with water at the bottom of a first seedling cultivation container (1), cutting the seedling cultivation materials according to the shape and size of the bottom of the first seedling cultivation container (1) before putting, then spreading bean seeds on the seedling cultivation materials, wherein the bean seeds are not overlapped, after spreading, overlapping the first seedling cultivation container (1), pressing a weight of 500g on the first seedling cultivation container (1) at the top, adjusting the vertical sequence of the first seedling cultivation container (1) every day until a tender bud with the length of 0.5cm grows out, removing the weight, horizontally arranging the first seedling cultivation container (1) on an experiment table, inoculating bean aphids when the tender buds grow to 3 +/-0.5 cm, after cultivating for 10d, cultivating the second seedling cultivation container (2) by the method of the first seedling cultivation container (1), and the quantity of the second seedling cultivation container (2) is 5 times of that of the first seedling cultivation container (1);
(2) inoculation of Aphis fabae
Collecting Aphis fabae on leguminous plants, returning the Aphis fabae to the room, transferring the Aphis fabae into a first seedling culture container (1) with tender shoots of 3 +/-0.5 cm, and placing the Aphis fabae on a seedling culture material;
(3) propagation of seed bean aphids
When bean seedlings in the first seedling raising container (1) are full of bean aphids, cutting the bean seedlings into small sections, wherein each section has 50 +/-5 aphids, transferring the bean seedling sections with the aphids into the first seedling raising containers (1) with newly-cultured tender shoots of 3 +/-0.5 cm in length, and transferring 500 +/-5 bean aphids into each first seedling raising container (1);
(4) aphis fabae propagation expanding method for propagating natural enemies
When bean aphids grow on bean seedlings in the first seedling raising container (1) in the expanding propagation of the bean aphids of provenance in the step (3), putting a support in the second seedling raising container (2), putting the first seedling raising container (1) on the support, taking out the first seedling raising container (1) and the support after 1 day, putting the first seedling raising container and the support in the new second seedling raising container (2), repeating for 5 times, and feeding the bean aphids for natural enemies in the second seedling raising container (2);
the medicament A is gibberellin, and the concentration of the prepared soak solution is 0.5 mg/L;
the bean is small red bean.
2. The indoor feeding method of myzus persicae as claimed in claim 1, wherein: the first seedling raising container (1) is a plastic pot with the diameter =10cm and the height =10 cm; the second seedling raising container (2) is a plastic pot with the diameter being more than or equal to 20cm and the height being =10 cm.
3. The indoor feeding method of myzus persicae as claimed in claim 1, wherein: the seedling raising material is bamboo fiber cloth, and the specification is that the length =25cm, and the width =25 cm.
4. The indoor feeding method of myzus persicae as claimed in claim 1, wherein: the support be stainless steel frame (3), the specification is diameter =9cm, and the height =4 cm.
CN201710006222.8A 2017-01-05 2017-01-05 Indoor raising method for bean aphids Expired - Fee Related CN106614408B (en)

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