CN201378218Y

CN201378218Y - Dual-purpose dish for culture and perfusion

Info

Publication number: CN201378218Y
Application number: CN200920099290U
Authority: CN
Inventors: 周晋; 王巍; 刘志宇
Original assignee: Individual
Current assignee: Individual
Priority date: 2009-03-13
Filing date: 2009-03-13
Publication date: 2010-01-06
Anticipated expiration: 2019-03-13

Abstract

The utility model provides a dual-purpose dish for culture and perfusion, which relates to a culture and perfusion dish. The dual-purpose dish aims at solving the problems that a special culture dish for a present laser scanning confocal microscope leads high processing difficulty, low finished product ratio as well as poor experiment precision and can easily incur siphoning. A dish wall plate and a dish bottom plate are manufactured into one body from top to bottom, a culture groove is arranged on the upper end face of the dish bottom plate, two perfusion grooves are also arranged on the upper end face of the dish bottom plate, the two perfusion grooves are symmetrically arranged along the axis of the culture groove and are respectively communicated with the culture groove, the depth h of the two perfusion grooves is smaller than the depth H of the culture groove, and the distance from the bottom face of the culture groove to the lower end face of the dish bottom plate is within 0.1 to 1mm. The dual-purpose dish is easy for processing, and the finished product ratio can reach more than 99.8%; the dual-purpose dish avoids losses of cell culture fluid, so as to make sure the pre-calculated medicine concentration is constant, thereby improving the experiment precision; moreover, the dual-purpose dish also effectively avoids the siphoning.

Description

Cultivate the dual-purpose ware of perfusion

Technical field

The utility model relates to a kind of cultivation perfusion ware.

Background technology

At present, the special-purpose double dish of laser scanning co-focusing microscope that medical circle generally adopts is to be circular hole 1-1 about 10mm opening a diameter on the base plate 1, stick with glue a thick cover glass 2 of 0.13mm～0.17mm again, form cylindrical cavity between the wall of circular hole 1-1 and the cover glass 2 and be used for cultured cell, the fluorescence probe mark also carries out (as shown in Figure 1) in cylindrical cavity.There is following shortcoming in above-mentioned double dish: one, because cover glass 2 is very thin and frangible, therefore bring difficulty to processing, yield rate is low; Two, the degree of depth of double dish is more shallow, and quantitatively the nutrient solution that adds very easily flows out, and then adds medicine in nutrient solution, because of nutrient solution flows out the drug concentration that calculating is good is in advance changed, thereby has reduced experimental precision; Three, the degree of depth of double dish is more shallow also can produce siphonage when perfusion.

The utility model content

The purpose of this utility model is in order to solve that the difficulty of processing that the special-purpose double dish of existing laser scanning co-focusing microscope exists is big, yield rate is low, experimental precision is low and easily to produce the problem of siphonage, and then provides a kind of cultivation perfusion dual-purpose ware.

The technical solution of the utility model is: cultivate the dual-purpose ware of perfusion and comprise the ware body, described ware body is made up of ware wallboard and ware base plate, described ware wallboard and ware base plate are made one from top to bottom, upper edge, the upper surface ware body of described ware base plate axially have a culture tank, upper edge, the upper surface ware body of described ware base plate axially also have two perfusion grooves, described two perfusion grooves are symmetrical arranged along the axis of culture tank, described two perfusion grooves all are communicated with culture tank, and the degree of depth h of two perfusion grooves is all less than the depth H of culture tank, and the bottom surface of described culture tank is 0.1mm～1mm to the lower surface of ware base plate apart from δ.

The utility model compared with prior art has following beneficial effect: the utility model is easy to processing by the die casting one-shot forming, and yield rate reaches more than 99.8%; Culture tank of the present utility model is communicated with two perfusion grooves, can regularly or carry out perfusion to culture tank at any time and change nutrient solution, liquid measure is cultivated in control, avoided the loss of nutrient solution, growth provides a good environment to cell, thereby it is constant to have guaranteed to calculate good drug concentration in advance, has improved experimental precision; Culture tank of the present utility model in addition is communicated with the generation of also effectively having avoided siphonage with two perfusion grooves.

Description of drawings

Fig. 1 is the structural representation of the special-purpose double dish of existing laser scanning co-focusing microscope, and Fig. 2 is that master of the present utility model looks cut-open view, and Fig. 3 is the vertical view of Fig. 2.

Embodiment

Embodiment one: the dual-purpose ware of cultivation perfusion of (referring to Fig. 2 and Fig. 3) present embodiment comprises ware body 3, described ware body 3 is made up of ware wallboard 4 and ware base plate 5, described ware wallboard 4 and ware base plate 5 are made one from top to bottom, upper edge, the upper surface ware body 3 of described ware base plate 5 axially have a culture tank 5-1, upper edge, the upper surface ware body 3 of described ware base plate 5 axially also have two perfusion groove 5-2, described two perfusion groove 5-2 are symmetrical arranged along the axis of culture tank 5-1, described two perfusion groove 5-2 all are communicated with culture tank 5-1, and the degree of depth h of two perfusion groove 5-2 is all less than the depth H of culture tank 5-1, and the bottom surface of described culture tank 5-1 is 0.1mm～1mm to the lower surface of ware base plate 5 apart from δ.

Embodiment two: the bottom surface of the culture tank 5-1 of (referring to Fig. 2) present embodiment is 0.13mm to the lower surface of ware base plate 5 apart from δ.So be provided with, experimental precision is higher, and the observation figure under the laser scanning co-focusing microscope is clear.Other composition is identical with embodiment one with annexation.

Embodiment three: the bottom surface of the culture tank 5-1 of (referring to Fig. 2) present embodiment is 0.17mm to the lower surface of ware base plate 5 apart from δ.So be provided with, experimental precision is higher, and the observation figure under the laser scanning co-focusing microscope is clear.Other composition is identical with embodiment one with annexation.

Embodiment four: the dual-purpose ware of cultivation perfusion of (referring to Fig. 2) present embodiment also increases ware lid 6, and described ware lid 6 lids are contained on the upper surface of ware body 3.So be provided with, effectively prevented the volatilization of medicine, avoided calculating good drug concentration in advance and changed, make experimental precision more accurate because of drug volatilization.Other composition is identical with embodiment one, two or three with annexation.

Adopt existing double dish and the utility model under identical condition to acute promyelocytic leukemia NB4 cell, use the tyrode's solution washed twice, Fluo-3/AM with 5 μ mol/L dyes then, add 0.03%PluronicF-127 again and in 37 ℃ of waters bath with thermostatic control, hatch 45min, then with tyrode's solution again washed twice remove the residual Fluo-3/AM in extracellular, under laser scanning co-focusing microscope, observe at last.Described laser scanning co-focusing microscope is produced by Japanese Olympus strain commercial firm (Olympus), and its model is Fluoview-FV300.Adopt image blurring that existing double dish obtains, the clear picture that adopts the utility model to obtain.

Claims

1, the dual-purpose ware of a kind of cultivation perfusion, it comprises ware body (3), it is characterized in that: described ware body (3) is made up of ware wallboard (4) and ware base plate (5), described ware wallboard (4) and ware base plate (5) are made one from top to bottom, upper edge, the upper surface ware body (3) of described ware base plate (5) axially have a culture tank (5-1), upper edge, the upper surface ware body (3) of described ware base plate (5) axially also have two perfusion grooves (5-2), described two perfusion grooves (5-2) are symmetrical arranged along the axis of culture tank (5-1), described two perfusion grooves (5-2) all are communicated with culture tank (5-1), and the degree of depth h of two perfusion grooves (5-2) is all less than the depth H of culture tank (5-1), and the bottom surface of described culture tank (5-1) is 0.1mm～1mm to the lower surface of ware base plate (5) apart from δ.

2, according to the dual-purpose ware of the described cultivation perfusion of claim 1, it is characterized in that: the bottom surface of described culture tank (5-1) is 0.13mm to the lower surface of ware base plate (5) apart from δ.

3, according to the dual-purpose ware of the described cultivation perfusion of claim 1, it is characterized in that: the bottom surface of described culture tank (5-1) is 0.17mm to the lower surface of ware base plate (5) apart from δ.

4, according to claim 1, the dual-purpose ware of 2 or 3 described cultivation perfusions, it is characterized in that: the dual-purpose ware of described cultivation perfusion also comprises ware lid (6), and described ware lid (6) lid is contained on the upper surface of ware body (3).