CN204129326U - The special slide of a kind of laser confocal microscope - Google Patents
The special slide of a kind of laser confocal microscope Download PDFInfo
- Publication number
- CN204129326U CN204129326U CN201420594224.5U CN201420594224U CN204129326U CN 204129326 U CN204129326 U CN 204129326U CN 201420594224 U CN201420594224 U CN 201420594224U CN 204129326 U CN204129326 U CN 204129326U
- Authority
- CN
- China
- Prior art keywords
- microslide
- lamellar body
- utility
- culture tank
- model
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Abstract
The utility model discloses the special slide of a kind of laser confocal microscope, comprise microslide lamellar body; Be located at the culture tank formed by the hot melt adhesive sprayed thereon of microslide lamellar body upper surface.Further, described culture tank is formed on the plane of described microslide lamellar body upper surface place.Further, the thickness of described microslide lamellar body is 0.13-0.17mm.Further, described hot melt adhesive is sprayed at described microslide lamellar body upper surface by thermosol gelgun.The utility model is exclusively used in laser co-focusing and other microscope, is specially adapted to the sample needing liquid handling when observing.Adopt the technical solution of the utility model, microslide lamellar body is carrier, when for observing the sample needing liquid handling, hot melt adhesive is sprayed at microslide lamellar body upper surface surround one close-shaped, namely form culture tank after hot melt adhesive coagulation forming.Compared with the prior art, the utility model can instantly obtain, and has significant cheapness and the advantage be conveniently easy to get.
Description
Technical field
The utility model relates to the special slide of a kind of laser confocal microscope, particularly relates to the special trough of belt slide of a kind of laser confocal microscope.
Background technology
Laser scanning confocal microscopy is widely used in biomedical research, uses middle sample to be placed in microslide or double dish, and laser need penetrate microslide or double dish arrives sample, and thus plastic products can not use without exception; Laser can only penetrate the ultra-thin glass of 0.17mm simultaneously, and thus simple glass double dish can not use.Certainly have special ultra-thin microslide, but a lot of biomedicine experiment needs the change observing living cells, thus will add reactant liquor in microslide or double dish, thus alone ultra-thin microslide is not all right.The conventional special ware of laser confocal microscope (being called for short the burnt special ware of copolymerization) as shown in Figure 1 and Figure 2, it is the glass guide channel 11 devising a ultra-thin glass material in a kind of plastic culture dish 10 bottom center, in Fig. 1, Reference numeral 12 represents the lid in cultivation face.Due to production technology more complicated, thus price is very expensive, the price 10-100 unit of the burnt special ware of copolymerization not etc., and single use.Thus, in laser co-focusing microexamination, the burnt special ware consumables cost of copolymerization costly, is difficult to the requirement meeting experiment.
Summary of the invention
Technical problem to be solved in the utility model is to provide a kind of laser confocal microscope cheap and easy to get special slide.
For solving the problems of the technologies described above, the technical scheme that the utility model is taked is:
The special slide of a kind of laser confocal microscope, comprises microslide lamellar body; Be located at the culture tank formed by the hot melt adhesive sprayed thereon of microslide lamellar body upper surface.
The utility model is exclusively used in laser co-focusing and other microscope, is specially adapted to the sample needing liquid handling when observing.Adopt the technical solution of the utility model, microslide lamellar body is carrier, when for observing the sample needing liquid handling, hot melt adhesive is sprayed at microslide lamellar body upper surface surround one close-shaped, namely form culture tank after hot melt adhesive coagulation forming.Afterwards, sample is put in the groove of slide as vegeto-animal section or other less sample, can freely add/sucking-off treating fluid carry out cleaning section or sample, and can not by section or sample wash away.And when carrying out long time treatment to section or sample, can ensure to cut into slices or sample is in a stable environment.Compared with the prior art, the special slide of the utility model laser confocal microscope can instantly obtain, and has significant cheapness and the advantage be conveniently easy to get.
Further, described culture tank is formed on the plane of described microslide lamellar body upper surface place.Due to the utility model, directly spraying hot-melt adhesive on microslide lamellar body upper surface, thus form culture tank.Do not need as prior art, borehole on microslide or double dish, to form glass guide channel, technique simplifies greatly, and cost significantly reduces, can be instant when carrying out confocal laser scanning microscope, convenient and practical.
Further, the thickness of described microslide lamellar body is 0.13-0.17mm, will too thickly cause laser to be difficult to penetrate, and also can not too thinly cause very fragile, thus more suitable, more well for confocal laser scanning microscope.
Further, described hot melt adhesive is sprayed at described microslide lamellar body upper surface by thermosol gelgun.The utility model can use conventional hot melt adhesive machine, is melted by hot melt rubber stick and is sprayed at microslide upper surface, namely bonds with microslide upper surface, and form culture tank after hot melt adhesive cooling.
Further, described culture tank is square, or described culture tank is circular or oval, or described culture tank is irregular close-shaped.As long as hot melt adhesive forms closed shape, thus treating fluid in the culture tank of hot melt adhesive formation, can not outflow.Concrete shape can experimentally need, and is randomly formed.
Accompanying drawing explanation
In order to be illustrated more clearly in the technical scheme of the utility model embodiment, be briefly described to the accompanying drawing of embodiment below.
Fig. 1 shows the stereographic map of the special ware of laser confocal microscope of the prior art;
Fig. 2 shows the cross-sectional figure of the special ware of laser confocal microscope of the prior art;
Fig. 3 is the vertical view of the special slide embodiment of the utility model laser confocal microscope;
Fig. 4 is cross-sectional figure embodiment illustrated in fig. 3;
Fig. 5 is the oblique view of the utility model embodiment, which show the treating fluid in culture tank;
Fig. 6 is the vertical view of another embodiment of the utility model;
Fig. 7 is the vertical view of the utility model embodiment again;
Fig. 8 is product pictorial diagram of the present utility model.
Fig. 9 is another product pictorial diagram of the present utility model, which show the treating fluid in culture tank.
Embodiment
Embodiment of the present utility model is further described below in conjunction with accompanying drawing.
Fig. 1, Fig. 2 display be the special ware of laser confocal microscope of prior art, it is the glass guide channel 11 devising a ultra-thin glass material in a kind of plastic culture dish 10 bottom center, generally speaking be bottom double dish 10, open a circular hole, stick with glue the glass sheet that can be used for laser scanning co-focusing again, the cylindrical cavity that the wall of circular hole is formed between the glass sheet of bottom and culture tank are for cultured cell.The special ware of such laser confocal microscope is because of its technics comparing complexity, and therefore price is more expensive, and cost is higher, is difficult to be applicable to a large amount of test demands.
The special slide embodiment of a kind of laser confocal microscope of the utility model is just like shown in Fig. 3,4,5, and it comprises microslide lamellar body 21; Be located at the culture tank 22 formed by the hot melt adhesive 23 sprayed thereon of microslide lamellar body 21 upper surface.The utility model embodiment is specially adapted to the sample needing liquid handling when observing.As shown in Figure 5, represented by dashed line in culture tank 22 treating fluid namely referred in culture tank.
It should be noted that, for hot melt adhesive of the present utility model, existing conventional hot melt adhesive can be adopted.So-called hot melt adhesive is a kind of plastic bonding agent, and in certain temperature range, its physical state changes with temperature change, and chemical characteristic is constant, and it is nonpoisonous and tasteless, belongs to environmental-protecting chemical product.Hot melt adhesive is generally by matrix resin, tackifier, plastifier, antioxidant, filler.Common hot melt adhesive, such as namely EVA hot-melt adhesive may be used in embodiment of the present utility model.
Adopt the technical solution of the utility model, microslide lamellar body 21 is carrier, when for observing the sample needing liquid handling, hot melt adhesive 23 is sprayed at microslide lamellar body 21 upper surface surround one close-shaped, in the present embodiment, culture tank 22 is namely formed after hot melt adhesive coagulation forming.Afterwards, sample is put in the culture tank 22 of slide as vegeto-animal section or other less sample, can freely add/sucking-off treating fluid carry out cleaning section or sample, and can not by section or sample wash away.And when carrying out long time treatment to section or sample, can ensure to cut into slices or sample is in a stable environment.Compared with the prior art, the special slide of the utility model laser confocal microscope can instantly obtain, and has significant cheapness and the advantage be conveniently easy to get.
Further, described culture tank 22 is formed on the plane of described microslide lamellar body 21 upper surface place.Due to the utility model directly spraying hot-melt adhesive 23 on microslide lamellar body 21 upper surface, thus form culture tank 22.Do not need as the prior art of Fig. 1, Fig. 2, borehole on microslide or double dish 11, to form glass guide channel 12, technique simplifies greatly, and cost significantly reduces, can be instant when carrying out confocal laser scanning microscope, convenient and practical.
Further, the thickness of described microslide lamellar body 21 is 0.13-0.17mm, will too thickly cause laser to be difficult to penetrate, and also can not too thinly cause very fragile, thus more suitable, more well for confocal laser scanning microscope.
Further, described hot melt adhesive 23 is sprayed at described microslide lamellar body 21 upper surface by thermosol gelgun.The utility model can use conventional hot melt adhesive machine, is melted by hot melt rubber stick and is sprayed at microslide upper surface, namely bonds with microslide upper surface, and form culture tank 22 after hot melt adhesive cooling.
Fig. 6 shows another embodiment of the utility model, and in this embodiment, the culture tank 32 formed by hot melt adhesive 33 of microslide lamellar body 31 upper surface, for circular, can certainly be oval.Or as Fig. 7 shown in another embodiment in, the culture tank 42 of microslide lamellar body 41 upper surface is irregular close-shaped.As long as hot melt adhesive forms closed shape, as long as hot melt adhesive 43 is formed close-shaped at microslide lamellar body 41, thus surround a closed groove, such treating fluid, in culture tank, can not outflow.Concrete shape can experimentally need, and is randomly formed.It should be noted that; in above-described embodiment of the present utility model; culture tank 22,32 is square, circular or oval; it is not needed to be accurate correspondingly-shaped; as long as be general square shape, circular or substantially elliptical, the change of shape will be understood that not affecting it falls into protection domain of the present utility model.
Fig. 8, Fig. 9 show product pictorial diagram, and Fig. 8 is product unused state, does not add treating fluid in culture tank; Fig. 9 is using state, adds treating fluid in culture tank.
The special slide embodiment of the utility model laser confocal microscope is cheap and be conveniently easy to get, and greatly reduces experiment equipment cost, highly promotes.
Above disclosedly be only preferred embodiment of the present utility model, certainly can not limit the interest field of the utility model with this, therefore according to the equivalent variations that the utility model claim is done, still belong to the scope that the utility model is contained.
Claims (7)
1. the special slide of laser confocal microscope, is characterized in that:
Comprise microslide lamellar body;
Be located at the culture tank formed by the hot melt adhesive sprayed thereon of microslide lamellar body upper surface.
2. the special slide of laser confocal microscope as claimed in claim 1, is characterized in that: described culture tank is formed on the plane of described microslide lamellar body upper surface place.
3. the special slide of laser confocal microscope as claimed in claim 1, is characterized in that: the thickness of described microslide lamellar body is 0.13-0.17mm.
4. the special slide of laser confocal microscope as claimed in claim 1, is characterized in that: described hot melt adhesive is sprayed at described microslide lamellar body upper surface by thermosol gelgun.
5. the special slide of laser confocal microscope as claimed in claim 1, is characterized in that: described culture tank is square.
6. the special slide of laser confocal microscope as claimed in claim 1, is characterized in that: described culture tank is for circular or oval.
7. the special slide of laser confocal microscope as claimed in claim 1, is characterized in that: described culture tank is irregular close-shaped.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201420594224.5U CN204129326U (en) | 2014-10-14 | 2014-10-14 | The special slide of a kind of laser confocal microscope |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201420594224.5U CN204129326U (en) | 2014-10-14 | 2014-10-14 | The special slide of a kind of laser confocal microscope |
Publications (1)
Publication Number | Publication Date |
---|---|
CN204129326U true CN204129326U (en) | 2015-01-28 |
Family
ID=52385669
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201420594224.5U Expired - Fee Related CN204129326U (en) | 2014-10-14 | 2014-10-14 | The special slide of a kind of laser confocal microscope |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN204129326U (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107015354A (en) * | 2017-05-10 | 2017-08-04 | 张学军 | A kind of reflective Laser Scanning Confocal Microscope paster |
CN109612936A (en) * | 2019-01-03 | 2019-04-12 | 陈茜 | A kind of coloured silk bottom fine flour laser specialized glass slide |
CN110673324A (en) * | 2019-09-27 | 2020-01-10 | 佛山科学技术学院 | Glass slide structure suitable for confocal microscope |
-
2014
- 2014-10-14 CN CN201420594224.5U patent/CN204129326U/en not_active Expired - Fee Related
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107015354A (en) * | 2017-05-10 | 2017-08-04 | 张学军 | A kind of reflective Laser Scanning Confocal Microscope paster |
CN109612936A (en) * | 2019-01-03 | 2019-04-12 | 陈茜 | A kind of coloured silk bottom fine flour laser specialized glass slide |
CN110673324A (en) * | 2019-09-27 | 2020-01-10 | 佛山科学技术学院 | Glass slide structure suitable for confocal microscope |
CN110673324B (en) * | 2019-09-27 | 2022-04-26 | 佛山科学技术学院 | Glass slide structure suitable for confocal microscope |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN204129326U (en) | The special slide of a kind of laser confocal microscope | |
WO2017216113A3 (en) | Cell culture device and methods | |
KR100836827B1 (en) | Cell culture dish for the embryoid body formation from embryonic stem cells | |
US9085792B2 (en) | Methods for inoculating culture media on Petri dishes by means of vibration frequencies | |
IN2014CN04599A (en) | ||
US20200154698A1 (en) | Fragile object preserving device provided with sealing mechanism | |
CN103255057B (en) | Micro-fluidic chip for cell culture as well as preparation method and application of micro-fluidic chip | |
WO2011116922A9 (en) | Thermoreactive substrate with microgels, method for its preparation, and culture method for biological cells | |
CN103710263A (en) | Cell culture apparatus | |
CN204790174U (en) | Do not need cover glass can directly be used for slide glass of film -making | |
CN203065491U (en) | Cell culture device | |
KR101480206B1 (en) | Three dimensional cell culturing method | |
CN203569075U (en) | PCR (polymerase chain reaction) plate cover | |
CN202139238U (en) | Micropore plate for microbiological assay | |
CN204752694U (en) | Burnt piece of cultivateing of copolymerization | |
Haring et al. | 3D bioprinting using hollow multifunctional fiber impedimetric sensors | |
WO2011113613A4 (en) | Array microinjection apparatuses and methods | |
CN108060059A (en) | A kind of simple fungal spore Observation On The Development device | |
CN203419927U (en) | PDMS (polydimethylsiloxane) based three-dimensional cell co-culture model | |
CN103330653A (en) | Crystal beautifying attachment film, preparation method and coating machine thereof | |
CN205258447U (en) | Cell chip based on inclusion and through hole | |
CN104309342A (en) | Seal applied to scratch test of cell | |
EP2992082B1 (en) | Device and method for dispensing a suspension of microorganisms | |
CN102943037A (en) | Multi-hole cell culture dish structure | |
CN101857837B (en) | Cell co-culture flow chamber |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150128 Termination date: 20161014 |