IN2014CN04599A - - Google Patents

Info

Publication number
IN2014CN04599A
IN2014CN04599A IN4599CHN2014A IN2014CN04599A IN 2014CN04599 A IN2014CN04599 A IN 2014CN04599A IN 4599CHN2014 A IN4599CHN2014 A IN 4599CHN2014A IN 2014CN04599 A IN2014CN04599 A IN 2014CN04599A
Authority
IN
India
Prior art keywords
culture medium
subjecting
serum
aggregate
signaling pathway
Prior art date
Application number
Inventor
Tokushige Nakano
Satoshi Ando
Yoshiki Sasai
Mototsugu Eiraku
Original Assignee
Sumitomo Chemical Co
Riken
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP2012043082A external-priority patent/JP5985209B2/en
Priority claimed from JP2012043083A external-priority patent/JP6067232B2/en
Priority claimed from JP2012043081A external-priority patent/JP5985208B2/en
Priority claimed from JP2012043080A external-priority patent/JP5985207B2/en
Application filed by Sumitomo Chemical Co, Riken filed Critical Sumitomo Chemical Co
Publication of IN2014CN04599A publication Critical patent/IN2014CN04599A/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/30—Nerves; Brain; Eyes; Corneal cells; Cerebrospinal fluid; Neuronal stem cells; Neuronal precursor cells; Glial cells; Oligodendrocytes; Schwann cells; Astroglia; Astrocytes; Choroid plexus; Spinal cord tissue
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
    • A61L27/3633—Extracellular matrix [ECM]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A61L27/3834—Cells able to produce different cell types, e.g. hematopoietic stem cells, mesenchymal stem cells, marrow stromal cells, embryonic stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3895—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells using specific culture conditions, e.g. stimulating differentiation of stem cells, pulsatile flow conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/062—Sensory transducers, e.g. photoreceptors; Sensory neurons, e.g. for hearing, taste, smell, pH, touch, temperature, pain
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0621—Eye cells, e.g. cornea, iris pigmented cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5058—Neurological cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/16—Materials or treatment for tissue regeneration for reconstruction of eye parts, e.g. intraocular lens, cornea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/70—Undefined extracts
    • C12N2500/80—Undefined extracts from animals
    • C12N2500/84—Undefined extracts from animals from mammals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/42—Notch; Delta; Jagged; Serrate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

Abstract

The present invention provides: [1] a method for producing a retinal tissue characterized by comprising steps (1) to (3) i.e. (1) a first step of subjecting a pluripotent stem cell to floating culture in a serum free culture medium containing a Wnt signaling pathway inhibitor to form an aggregate of the pluripotent stem cell (2) a second step of subjecting the aggregate formed in the first step to floating culture in a serum free culture medium containing a basement membrane preparation and (3) a third step of subjecting the aggregate cultured in the second step to floating culture in a serum containing culture medium; [2] a method for producing an optic cup like structure characterized by comprising a step of subjecting the aggregate containing a retinal tissue which has been cultured in the third step mentioned above to floating culture in a serum free culture medium or a serum containing culture medium each containing a substance capable of acting on the Sonic hedgehog signaling way and a substance capable of acting on the Wnt signaling pathway; [3] a method for producing a retinal pigment epithelial cell characterized by comprising a step of subjecting the aggregate containing a retinal tissue which has been cultured in the third step mentioned above to floating culture in a serum free culture medium or a serum containing culture medium each containing a substance capable of acting on the Wnt signaling pathway (wherein the serum free culture medium and the serum containing culture medium does not contain any substance capable of acting on the Sonic hedgehog signaling pathway); and [4] a method for producing a retinal layer specific nerve cell characterized by comprising bringing a retinal progenitor cell contained in a retinal tissue derived from a primate pluripotent stem cell into contact with a Notch signaling pathway inhibitor.
IN4599CHN2014 2011-11-25 2012-11-22 IN2014CN04599A (en)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
JP2011258209 2011-11-25
JP2011258210 2011-11-25
JP2011258211 2011-11-25
JP2011258212 2011-11-25
JP2012043082A JP5985209B2 (en) 2011-11-25 2012-02-29 Manufacturing method of eyecup-like structure
JP2012043083A JP6067232B2 (en) 2011-11-25 2012-02-29 Method for producing retinal layer-specific neurons
JP2012043081A JP5985208B2 (en) 2011-11-25 2012-02-29 Method for manufacturing retinal tissue
JP2012043080A JP5985207B2 (en) 2011-11-25 2012-02-29 Method for producing retinal pigment epithelial cells
PCT/JP2012/080366 WO2013077425A1 (en) 2011-11-25 2012-11-22 Methods for producing retinal tissue and retina-related cell

Publications (1)

Publication Number Publication Date
IN2014CN04599A true IN2014CN04599A (en) 2015-09-18

Family

ID=51221042

Family Applications (1)

Application Number Title Priority Date Filing Date
IN4599CHN2014 IN2014CN04599A (en) 2011-11-25 2012-11-22

Country Status (9)

Country Link
US (2) US10973854B2 (en)
EP (1) EP2784152B1 (en)
KR (1) KR102111936B1 (en)
CN (2) CN113817679A (en)
AU (1) AU2012341417B2 (en)
CA (2) CA3114895A1 (en)
ES (1) ES2733956T3 (en)
IN (1) IN2014CN04599A (en)
WO (1) WO2013077425A1 (en)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2012341417B2 (en) * 2011-11-25 2018-06-28 Riken Methods for producing retinal tissue and retina-related cell
WO2013183774A1 (en) 2012-06-08 2013-12-12 住友化学株式会社 Method for producing ciliary marginal zone-like structure
US11241460B2 (en) 2013-03-15 2022-02-08 Astellas Institute For Regenerative Medicine Photoreceptors and photoreceptor progenitors produced from pluripotent stem cells
CA2922079C (en) 2013-08-23 2022-07-05 Sumitomo Chemical Company, Limited Method for producing retinal tissue and retina-related cells
AU2014345110B2 (en) 2013-11-11 2021-01-14 Riken Method for producing retinal pigment epithelial cells
MX2016006915A (en) 2013-11-27 2017-01-23 Kyoto Prefectural Public Univ Corp Application of laminin to corneal endothelial cell culture.
JP6495830B2 (en) 2013-12-11 2019-04-03 住友化学株式会社 Method for producing ciliary peripheral edge-like structure
US11724005B2 (en) 2014-01-17 2023-08-15 Sumitomo Chemical Company, Limited Method for manufacturing ciliary margin stem cells
US10280400B2 (en) 2014-05-05 2019-05-07 Indiana University Research And Technology Corporation Method for producing retinal ganglion cells from pluripotent cells
EP3178925B1 (en) 2014-08-08 2021-10-06 National Center For Child Health And Development Method for producing retinal ganglion cells
ES2880353T3 (en) * 2014-10-24 2021-11-24 Sumitomo Dainippon Pharma Co Ltd Retinal tissue production method
WO2016063985A1 (en) * 2014-10-24 2016-04-28 大日本住友製薬株式会社 Production method for nerve tissue
CN107073067A (en) 2014-10-31 2017-08-18 京都府公立大学法人 Use the treatment of the new cornea of laminin
EP3213761B1 (en) 2014-10-31 2021-05-19 Kyoto Prefectural Public University Corporation Novel treatment of retina using laminin
EP3348631B1 (en) * 2015-09-08 2023-11-29 Sumitomo Pharma Co., Ltd. Method for producing retinal tissue
CN106754636B (en) * 2015-11-19 2019-08-30 中国人民解放军第二军医大学 External evoked primary hepatocyte bile ductization and long-term cultivation, amplification and the method and its application of differentiation
AU2017254264C1 (en) * 2016-04-22 2023-10-05 Riken Method for producing retinal tissue
AU2017363689B2 (en) * 2016-11-25 2023-11-23 Riken Cell population for transplantation and method for producing same
EP3332695B1 (en) * 2016-12-09 2021-05-26 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Measurement device
JP2020519379A (en) * 2017-05-08 2020-07-02 ラムダヴィジョン インコーポレイテッド Methods for stimulating retinal cells and treating vision loss
KR20200029479A (en) 2017-07-20 2020-03-18 고쿠리쓰 겐큐 가이하쓰 호징 리가가쿠 겐큐소 Methods of preservation of nerve tissue
CN110945119B (en) * 2017-07-20 2024-04-05 国立研究开发法人理化学研究所 Method for maturation of retinal tissue comprising continuous epithelium
WO2019050015A1 (en) 2017-09-08 2019-03-14 国立研究開発法人理化学研究所 Cell aggregate including retinal tissue and production method therefor
EP3683306A4 (en) 2017-09-14 2021-06-09 Riken Method for producing retinal tissues
JPWO2019054515A1 (en) 2017-09-14 2020-10-15 国立研究開発法人理化学研究所 Method of increasing pyramidal photoreceptors or rod photoreceptors by dorsal or ventral signaling
SG11202004831QA (en) * 2017-11-24 2020-06-29 Sumitomo Chemical Co Production method for cell mass including neural cells/tissue and non-neural epithelial tissue, and cell mass from same
US20210002607A1 (en) * 2018-03-07 2021-01-07 Sorbonne Universite Compositions and Methods for Efficient Amplification of Retinal Progenitors Cells
US20200149003A1 (en) * 2018-04-20 2020-05-14 Miami University Methods for the production of retinal cells
KR102142400B1 (en) * 2018-06-19 2020-08-07 서울대학교산학협력단 Medium composition for culturing porcine pluripotent stem cell
WO2020138430A1 (en) 2018-12-28 2020-07-02 国立研究開発法人理化学研究所 Therapeutic drug for disease accompanied by disorders in retinal system cells or retinal tissue
WO2020184720A1 (en) 2019-03-13 2020-09-17 大日本住友製薬株式会社 Method for evaluating quality of transplant neural retina, and transplant neural retina sheet
US20220195383A1 (en) * 2019-03-29 2022-06-23 Kaneka Corporation Method for producing pluripotent stem cells
JPWO2020218480A1 (en) 2019-04-26 2020-10-29
CN116157512A (en) 2020-09-11 2023-05-23 国立研究开发法人理化学研究所 Composite comprising cell aggregate containing neural retina and matrix, and method for producing same
WO2022054924A1 (en) 2020-09-11 2022-03-17 大日本住友製薬株式会社 Medium for tissue for transplantation
CN116445409B (en) * 2023-06-16 2023-09-22 中国人民解放军总医院第三医学中心 Preparation method and application of rod photoreceptor precursor cells

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001508302A (en) 1997-01-10 2001-06-26 ライフ テクノロジーズ，インコーポレイテッド Embryonic stem cell serum replacement
UA79753C2 (en) * 2001-07-27 2007-07-25 Curis Inc Mediators of hedgehog signaling pathways, compositions and uses related thereto
US8492147B2 (en) 2004-06-18 2013-07-23 Riken Method of inducing the differentiation of embryonic stem cells into nerve by serum-free suspension culture
EP2808782A1 (en) 2004-12-24 2014-12-03 IZUTSU, Masahiro Mobile information communication apparatus, connection unit for mobile information communication apparatus, and external input/output unit for mobile information communication apparatus
US7541186B2 (en) * 2006-02-22 2009-06-02 University Of Washington Method of generating human retinal progenitors from embryonic stem cells
JP5441099B2 (en) 2007-01-18 2014-03-12 独立行政法人理化学研究所 Method for inducing differentiation into photoreceptor cells
KR100910517B1 (en) 2007-03-21 2009-07-31 엔에이치엔비즈니스플랫폼 주식회사 System and method for expanding target inventory according to borwser-login mapping
EP2147094B2 (en) 2007-04-18 2018-02-21 Hadasit Medical Research Services & Development Limited Stem cell-derived retinal pigment epithelial cells
PL2209888T3 (en) * 2007-10-12 2020-05-18 Astellas Institute For Regenerative Medicine Improved methods of producing rpe cells and compositions of rpe cells
CA2771901C (en) 2009-08-24 2020-03-10 Wisconsin Alumni Research Foundation Substantially pure human retinal progenitor, forebrain progenitor, and retinal pigment epithelium cell cultures and methods of making the same
KR101268741B1 (en) * 2009-10-06 2013-06-04 김지연 Method for differentiation into retinal cells from stem cells
WO2011043592A2 (en) * 2009-10-06 2011-04-14 Snu R&Db Foundation Compositions for inducing differentiation into retinal cells from retinal progenitor cells or inducing proliferation of retinal cells comprising wnt signaling pathway activators
JP5787370B2 (en) * 2009-11-05 2015-09-30 国立研究開発法人理化学研究所 Stem cell differentiation induction method
JP5505192B2 (en) 2010-08-17 2014-05-28 富士通株式会社 Competitive test equipment
JP5403374B2 (en) 2010-08-17 2014-01-29 カシオ計算機株式会社 Electronics
JP2012043081A (en) 2010-08-17 2012-03-01 Seiko Epson Corp Electronic apparatus and information management system
JP5094932B2 (en) 2010-08-17 2012-12-12 ヤフー株式会社 Information display apparatus, method and program
JP5377580B2 (en) 2011-06-23 2013-12-25 株式会社 テクスピアー Authentication device for back of hand and authentication method for back of hand
JP2011258211A (en) 2011-07-05 2011-12-22 Fujitsu Component Ltd Sound output device
AU2012341417B2 (en) * 2011-11-25 2018-06-28 Riken Methods for producing retinal tissue and retina-related cell
CA2922079C (en) * 2013-08-23 2022-07-05 Sumitomo Chemical Company, Limited Method for producing retinal tissue and retina-related cells
AU2014345110B2 (en) * 2013-11-11 2021-01-14 Riken Method for producing retinal pigment epithelial cells
EP3178925B1 (en) * 2014-08-08 2021-10-06 National Center For Child Health And Development Method for producing retinal ganglion cells
ES2880353T3 (en) * 2014-10-24 2021-11-24 Sumitomo Dainippon Pharma Co Ltd Retinal tissue production method
AU2017254264C1 (en) * 2016-04-22 2023-10-05 Riken Method for producing retinal tissue

Also Published As

Publication number Publication date
US20210228645A1 (en) 2021-07-29
CN113817679A (en) 2021-12-21
KR20140097442A (en) 2014-08-06
US20140341864A1 (en) 2014-11-20
CA3114895A1 (en) 2013-05-30
EP2784152A4 (en) 2015-11-11
CA2856867C (en) 2021-06-01
EP2784152B1 (en) 2019-04-10
CN103998599A (en) 2014-08-20
US10973854B2 (en) 2021-04-13
KR102111936B1 (en) 2020-05-18
ES2733956T3 (en) 2019-12-03
AU2012341417B2 (en) 2018-06-28
CA2856867A1 (en) 2013-05-30
AU2012341417A1 (en) 2014-06-26
WO2013077425A1 (en) 2013-05-30
EP2784152A1 (en) 2014-10-01

Similar Documents

Publication Publication Date Title
IN2014CN04599A (en)
AU2017254264A1 (en) Method for producing retinal tissue
EA201390801A1 (en) CULTIVATION METHOD FOR MAINTAINING AND POPULATIONS pure or enriched neural stem cell and / or neural progenitor cells of mammals prone to DIFFERENTIATION WITH FORMATION OF CELL LINES IN VITRO OLIGODENDROTSITARNOY
MY191740A (en) Production method for nerve tissue
AU2019268194A1 (en) Cell culture system
MY183058A (en) Production method for retinal tissue
WO2009028631A1 (en) Agent for promoting corneal endothelial cell adhesion
RU2011121843A (en) DIFFERENTIATION OF HUMAN EMBRYONAL STEM CELLS IN THE LINE OF PANCREATIC ENDOCRINE CELLS
WO2010096588A3 (en) Cell culture media containing combinations of proteins
MX348612B (en) Microorganisms and methods for producing 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol and related alcohols.
MY156588A (en) Systems using cell culture for production of isoprene
ES2523998T3 (en) Dermal cytoblast isolation method
WO2009023525A3 (en) Methods of modulating mesenchymal stem cell differentiation
WO2009117439A3 (en) Combined chemical and genetic approaches for generation of induced pluripotent stem cells
MX356756B (en) Pluripotent stem cell culture on micro-carriers.
EP1789534A4 (en) Isolation of stem/progenitor cells from amniotic membrane of umbilical cord.
WO2010107192A3 (en) Isolating method for umbilical cord blood-derived pluripotent stem cells expressing znf281
MX2015008577A (en) Culturing of human embryonic stem cells at the air-liquid interface for differentiation into pancreatic endocrine cells.
MX348269B (en) Method of producing retinal pigment epithelial cell sheet.
BR112022017216A2 (en) METHOD TO PRODUCE NATURAL EXTERMINATOR CELLS FROM PLURIPOTENT STEM CELLS
WO2007143117A3 (en) Differentiation of primate pluripotent cells to hepatocyte-lineage cells
WO2012092542A3 (en) Responsive cell culture hydrogel
WO2010020876A3 (en) Cell culture method to form aggregates
JP2012143229A5 (en)
WO2009117098A3 (en) Methods for stem cell production and therapy