CN101857837B - Cell co-culture flow chamber - Google Patents
Cell co-culture flow chamber Download PDFInfo
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- CN101857837B CN101857837B CN2010102163537A CN201010216353A CN101857837B CN 101857837 B CN101857837 B CN 101857837B CN 2010102163537 A CN2010102163537 A CN 2010102163537A CN 201010216353 A CN201010216353 A CN 201010216353A CN 101857837 B CN101857837 B CN 101857837B
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Abstract
The invention relates to a cell co-culture flow chamber in the biomechanic research field. The cell co-culture flow chamber is composed of a polystyrene base, a double-sided adhesive and a polycarbonate upper plate, wherein the polycarbonate upper plate comprises a co-culture cup placing hole, a co-culture cup fixing grooves, liquid buffer grooves, an inlet, an outlet and metal joints, wherein the co-culture cup fixing grooves are protruded from the co-culture cup placing hole and arranged at both sides of the co-culture cup placing hole, the two liquid buffer grooves are respectively arranged in the left side and the right side on the lower plane of the polycarbonate upper plate and in the middle of the edge of the co-culture cup placing hole and the edge of the polycarbonate upper plate, and the inlet and the outlet are arranged inside the polycarbonate upper plate and are connected with the metal joints and vertically communicated with the liquid buffer grooves; and the middle of the polystyrene base is provided with an observation hole on which a glass slide covers, the edges at both sides of the base is symmetrically provided with two metal joint fixing holes. The cell co-culture flow chamber can facilitate real-time microscopic observation and research on cell electrophysiological patch clamp technology.
Description
Technical field
What the present invention relates to is a kind of cell cultures device of biological technical field, specifically is a kind of cell co-culture flow chamber that is used for real-time microscopic examination.
Background technology
Flow cavity is one of people's main tool of being used in vitro study cell mechanical behavior under the shearing stress effect.The flow cavity that is usually used in biomechanics Research generally is divided into two types: two kinds of parallel flat single cell culture flow cavity and parallel flat cell co-culture flow chambers.Its work characteristics of flow cavity that is used for single cell culture is to study the influence of fluid shear stress to a kind of cell, and because of its function and technical difficulty are comparatively simple, there is certain limitation its application scenario, can not realize complicated research purpose.The flow cavity that cell is united cultivation is to be used to study the influence of fluid shear stress to two kinds of cell co-cultivation; Must make an ability and cell because of it unites and cultivates cup uniting of coupling cultivated a cup putting hole fully; It designs and produces precision and has relatively high expectations relatively, can be used in bicelluar repercussion study under the fluid shear stress effect.
Retrieval through to prior art finds that utilization parallel flat flow chambers such as Han Qin Wang apply mechanical response research (Han Qin Wang, the Lang Xian Huan that fluid shear stress is carried out cell to the associating culturing cell; Ming Juan Qu, Zhi Qiang Yan, Bo Liu; Bao Rong Shen, and Zong Lai Jiang (2006) Shear Stress Protects against Endothelial Regulation of Vascular Smooth Muscle Cell Migration in a Coculture System.Endothelium, 13; 171-180); This flow cavity utilizes upper and lower two parallel flats, and its upper plate central authorities have to unite cultivates the cup putting hole, and two ends of lower plate respectively have a fluid cushion groove; And the vertical direction of fluid cushion groove respectively has a gangway to be attached thereto logical; Process a rectangular cavities with certain thickness pellosil between the upper and lower plates, upper and lower plates is fixed with the metal screw of some amount, kind is had cultivate cup uniting of two kinds of cells and place to unite and cultivate a cup putting hole; Liquid with certain flow rate gets into rectangular cavities from inlet through the fluid cushion groove; Process outlet dashpot flows out from outlet, implements to apply the different fluids shearing stress, the mechanical response and the interaction of research cell to being planted in the cell of uniting cultivation cup bottom surface.
But this prior art can not be carried out Real Time Observation at microscopically owing to receive the influence of slab-thickness, and assembling is had relatively high expectations before the experiment, and than effort and time-consuming.Along with the continuous expansion of cyto-mechanics research field and deeply, need a kind ofly new can be used for real-time microscopic examination and carry out that the cellular electrophysiologicalsensor patch clamp technique is studied, easy to operate, slim and graceful and practical cell and unite the flow cavity of cultivation.
Summary of the invention
The present invention is directed to the above-mentioned deficiency that prior art exists, a kind of cell co-culture flow chamber is provided, make it have characteristics such as easy to operate, slim and graceful and practicality.
The present invention realizes that through following technical scheme the present invention adopts three-decker, comprises PS base, double faced adhesive tape and polycarbonate upper plate.Wherein the polycarbonate upper plate is arranged in the PS base, and double faced adhesive tape is close between polycarbonate upper plate and the PS base.
The middle of described PS base is provided with a circular vision slit, covers a block length quadrate slide in the base, and the both sides of the edge of base are arranged with two metal joint fixed orificess.
Described polycarbonate upper plate comprises uniting to be cultivated a cup putting hole, unites and cultivate a cup pickup groove, fluid cushion groove, inlet, outlet and metal joint.Unite and cultivate the cup pickup groove and protrude to unite and cultivate the cup putting hole and uniting the both sides of cultivating the cup putting hole; About the fluid cushion groove each one, be located on the polycarbonate upper plate lower plane and cultivate position intermediate between cup putting hole edge and the polycarbonate upper board edge uniting.Entrance and exit is located at intralamellar part on the polycarbonate, is connected with metal joint and is connected with the fluid cushion groove is vertical.
Described fluid cushion groove is a rectangular cavities.
Be provided with screw thread in the described entrance and exit.
Described double faced adhesive tape is provided with a rectangular cavity therebetween, and the width of rectangular cavity equals to unite the diameter of cultivating the cup putting hole, and the length of rectangular cavity is two distances between the fluid cushion groove center point.
Described metal joint is connected with entrance and exit on the polycarbonate upper plate through the metal joint fixed orifices on the PS base.
The described associating cultivates the cup pickup groove and cultivates the cup putting hole and constitute a shape and be matched with fully and unite the circular configuration of cultivating cup with uniting.
Thick 0.13 millimeter of described slide; Thick 150 microns of described double faced adhesive tape.
It is easy that the present invention has assembling; Merrily and lightheartedly, practicality, cost of manufacture is low; Because uniting cultivation glass putting hole and uniting the circular configuration of uniting the cultivation cup that is matched with of cultivating the formation of cup pickup groove on the polycarbonate upper plate; Leave enough positions of the operation of electrode in the patch clamp technique, so it can carry out the electrode operation in the cellular electrophysiologicalsensor patch clamp technique easily, and the present invention has replaced the lower plate of parallel flat flow chamber with slide; And in base, be provided with a vision slit, convenient Real Time Observation of carrying out the cell strained condition at microscopically.
Description of drawings
Fig. 1 is a two dimensional structure synoptic diagram of the present invention;
Fig. 2 is a working state figure of the present invention;
Wherein figure (a) is an anterior view, and figure (b) is an antapical view.
Embodiment
Elaborate in the face of embodiments of the invention down, present embodiment provided detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment being to implement under the prerequisite with technical scheme of the present invention.
As shown in Figure 1, present embodiment adopts three-decker, comprises PS base 1, double faced adhesive tape 2 and polycarbonate upper plate 3, and wherein polycarbonate upper plate 3 is arranged in the PS base 1, and double faced adhesive tape 2 is close between polycarbonate upper plate 3 and the PS base 1.
Described polycarbonate upper plate 3 comprises metal joint 4, inlet 5, outlet 6, fluid cushion groove 7, unites a cultivation cup putting hole 8, unites and cultivate cup pickup groove 9; Wherein unite and cultivate cup pickup groove 9 and protrude to unite and cultivate cup putting hole 8 and uniting the both sides of cultivating cup putting hole 8; Unite cultivation cup pickup groove 9 and unite cultivation cup putting hole 8 formations one form fit in uniting the circular configuration of cultivating cup; Respectively one of fluid cushion groove about 7; Be rectangular cavities, be located on polycarbonate upper plate 3 lower planes and cultivate position intermediate between cup putting hole 8 edges and polycarbonate upper plate 3 edges uniting, inlet 5 with export 6 and be located at polycarbonate upper plate 3 inside; Inlet 5 is established screw thread with outlet in 6, be connected with metal joint 4 and with 7 vertical being connected of fluid cushion groove.
Described double faced adhesive tape 2, its thickness are 150 microns, form one therebetween and widely cultivate cup putting hole 8 diameter for uniting, and length is apart from the rectangular cavity of length between two fluid cushion groove 7 central positions.
Described PS base 1 heart position therein is provided with a circular vision slit 10; The vision slit diameter is not less than microscope camera lens size; And use a block length to be 60mm; Wide is 50mm, and thickness is that the slide of 0.13mm covers above the vision slit 10, is provided with the metal joint fixed orifices 12 of joint access 5 and outlet 6 in PS base 1 both sides of edges symmetric position.
The one side of described double faced adhesive tape 2 is pasted on the bottom surface of polycarbonate upper plate 3; The width of its inner rectangular cavity is coincide with the diameter of uniting cultivation cup putting hole 8 fully; Distance between the length of rectangular cavity and two fluid cushion groove 7 central points is coincide; Another side is pasted on the slide 11 in the PS base 1, two metal joints 4 through the inlet 5 on the metal joint fixed orifices 12 on the PS base 1 and the polycarbonate upper plate 3 with export 6 and be connected.
As shown in Figure 2; There is the cultivation cup of uniting of cell to cultivate in the cup putting hole 8 kind in uniting; Liquid with certain flow rate is through inlet 5; The fluid cushion of overflowing groove 7 gets into outlet 6 through rectangular cavities fluid cushion groove 7 flows out from exporting 6, thereby implements to load fluid shear stress constantly for the associating cultured cells, carries out the correlative studys such as cellular electrophysiologicalsensor under the mechanics loading environment at microscopically utilization patch clamp technique pair cell simultaneously.
Claims (5)
1. cell co-culture flow chamber; Constitute by PS base, double faced adhesive tape and polycarbonate upper plate; It is characterized in that; Adopt the three-decker of PS base, double faced adhesive tape and polycarbonate upper plate, the polycarbonate upper plate is arranged in the PS base, and double faced adhesive tape is close between polycarbonate upper plate and the PS base; Described polycarbonate upper plate comprises uniting to be cultivated a cup putting hole, unites and cultivate a cup pickup groove, fluid cushion groove; Inlet, outlet and metal joint; Unite and cultivate the cup pickup groove and protrude to unite and cultivate the cup putting hole and uniting the both sides of cultivating the cup putting hole; About the fluid cushion groove each one; Be located on the polycarbonate upper plate lower plane and cultivate position intermediate between cup putting hole edge and the polycarbonate upper board edge uniting, entrance and exit is located at intralamellar part on the polycarbonate, is connected with metal joint and is connected with the fluid cushion groove is vertical; The described associating cultivates the cup pickup groove and cultivates the cup putting hole and constitute a shape and be matched with fully and unite the circular configuration of cultivating cup with uniting; The middle of described PS base is provided with a circular vision slit, and the vision slit top covers a block length quadrate slide, and the both sides of the edge of base are arranged with two metal joint fixed orificess; Described metal joint is connected with entrance and exit on the polycarbonate upper plate through the metal joint fixed orifices on the PS base; Described double faced adhesive tape is provided with a rectangular cavity in the centre, the width of rectangular cavity equals to unite the diameter of cultivating the cup putting hole, and the length of rectangular cavity is two distances between the fluid cushion groove center point; The one side of said double faced adhesive tape is pasted on the bottom surface of polycarbonate upper plate; The width of its inner rectangular cavity is coincide with the diameter of uniting cultivation cup putting hole fully; Distance between the length of rectangular cavity and two fluid cushion groove center points is coincide, and another side is pasted on the slide in the PS base.
2. cell co-culture flow chamber according to claim 1 is characterized in that, described fluid cushion groove is a rectangular cavities.
3. cell co-culture flow chamber according to claim 1 is characterized in that, is provided with screw thread in the described entrance and exit.
4. cell co-culture flow chamber according to claim 1 is characterized in that, thick 0.13 millimeter of described slide.
5. cell co-culture flow chamber according to claim 1 is characterized in that, thick 150 microns of described double faced adhesive tape.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102163537A CN101857837B (en) | 2010-07-02 | 2010-07-02 | Cell co-culture flow chamber |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102163537A CN101857837B (en) | 2010-07-02 | 2010-07-02 | Cell co-culture flow chamber |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101857837A CN101857837A (en) | 2010-10-13 |
| CN101857837B true CN101857837B (en) | 2012-08-22 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010102163537A Expired - Fee Related CN101857837B (en) | 2010-07-02 | 2010-07-02 | Cell co-culture flow chamber |
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| Country | Link |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102296030B (en) * | 2011-08-15 | 2013-06-19 | 上海交通大学 | Flow chamber for loading disturbance flow on co-cultured cells |
| CN112430521A (en) * | 2020-11-27 | 2021-03-02 | 无锡费曼科技有限公司 | Fluid observation vessel for observing microbial activity |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2486558Y (en) * | 2001-07-18 | 2002-04-17 | 中国科学院力学研究所 | External cell cultivation parallel round plate flow cavity |
| CN2486559Y (en) * | 2001-07-18 | 2002-04-17 | 中国科学院力学研究所 | Non-parallel plate flow cavity in external cell cultivator |
| CN2516990Y (en) * | 2001-10-23 | 2002-10-16 | 中国科学院生物物理研究所 | Sample viewing device for microscope |
| CN101520960A (en) * | 2009-03-31 | 2009-09-02 | 四川大学 | Experimental device for in-vitro simulated blood vessel microenvironment |
-
2010
- 2010-07-02 CN CN2010102163537A patent/CN101857837B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2486558Y (en) * | 2001-07-18 | 2002-04-17 | 中国科学院力学研究所 | External cell cultivation parallel round plate flow cavity |
| CN2486559Y (en) * | 2001-07-18 | 2002-04-17 | 中国科学院力学研究所 | Non-parallel plate flow cavity in external cell cultivator |
| CN2516990Y (en) * | 2001-10-23 | 2002-10-16 | 中国科学院生物物理研究所 | Sample viewing device for microscope |
| CN101520960A (en) * | 2009-03-31 | 2009-09-02 | 四川大学 | Experimental device for in-vitro simulated blood vessel microenvironment |
Non-Patent Citations (1)
| Title |
|---|
| 丛兴忠等.用于内皮细胞与平滑肌细胞联合培养的流动腔系统.《医用生物力学》.2001,第16卷(第1期),1-5. * |
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| Publication number | Publication date |
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| CN101857837A (en) | 2010-10-13 |
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