CN112430521A - Fluid observation vessel for observing microbial activity - Google Patents
Fluid observation vessel for observing microbial activity Download PDFInfo
- Publication number
- CN112430521A CN112430521A CN202011355474.XA CN202011355474A CN112430521A CN 112430521 A CN112430521 A CN 112430521A CN 202011355474 A CN202011355474 A CN 202011355474A CN 112430521 A CN112430521 A CN 112430521A
- Authority
- CN
- China
- Prior art keywords
- storage cavity
- liquid storage
- cavity seat
- liquid
- base
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000012530 fluid Substances 0.000 title claims abstract description 17
- 230000000813 microbial effect Effects 0.000 title claims abstract description 14
- 239000007788 liquid Substances 0.000 claims abstract description 64
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims abstract description 43
- 229910052710 silicon Inorganic materials 0.000 claims abstract description 43
- 239000010703 silicon Substances 0.000 claims abstract description 43
- 238000007789 sealing Methods 0.000 claims abstract description 8
- 239000000463 material Substances 0.000 claims description 14
- 239000011521 glass Substances 0.000 claims description 6
- 239000010453 quartz Substances 0.000 claims description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 6
- 238000000034 method Methods 0.000 claims 1
- 244000005700 microbiome Species 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 5
- 239000011550 stock solution Substances 0.000 description 10
- 239000012295 chemical reaction liquid Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
Images
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
- B01L9/52—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
Abstract
The invention relates to a fluid observation vessel for observing microbial activity, which comprises a base, a liquid storage cavity seat, a liquid inlet pipe, a liquid outlet pipe, a pressing plate, a fastening bolt, a transparent cover plate, a silicon wafer placing column, a silicon wafer and a sealing ring, wherein the liquid storage cavity seat is arranged on the base; the silicon chip placing device comprises a base, a liquid storage cavity seat, a liquid storage cavity, a silicon chip placing column, a silicon chip, a liquid inlet pipe, a liquid outlet pipe, a pressure plate and a transparent cover plate, wherein the liquid storage cavity seat is arranged on the upper surface of the base, the liquid storage cavity is internally provided with a liquid storage cavity with an opening at the upper end, the bottom plate of the liquid storage cavity seat is provided with the silicon chip placing column, the upper end surface of the silicon chip placing column is provided with the silicon chip, the two sides of the lower end part of the liquid storage cavity seat are respectively fixed with the liquid inlet pipe and the liquid outlet pipe, the upper end surface of the liquid storage cavity seat is provided with the pressure plate, the liquid storage cavity seat is tightly fixed. The invention can well observe the activity of microorganisms and prevent the interference of external factors, and has simple structure and convenient use.
Description
Technical Field
The invention relates to a microorganism microscopic observation device, in particular to a fluid observation vessel for observing microorganism activity.
Background
A fluid observation vessel is a device used for observing the change of microbial activity on the surface of a two-dimensional material in different fluids.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provide a fluid observation vessel for observing microbial activity, which has a simple structure and is convenient to use and capable of preventing interference of external factors.
According to the technical scheme provided by the invention, the fluid observation vessel for observing microbial activity comprises a base, a liquid storage cavity seat, a liquid inlet pipe, a liquid outlet pipe, a pressing plate, a fastening bolt, a transparent cover plate, a silicon wafer placing column, a silicon wafer and a sealing ring; the silicon chip placing device comprises a base, a liquid storage cavity seat, a liquid storage cavity, a silicon chip placing column, a silicon chip, a liquid inlet pipe, a liquid outlet pipe, a pressure plate and a transparent cover plate, wherein the liquid storage cavity seat is arranged on the upper surface of the base, the liquid storage cavity is internally provided with a liquid storage cavity with an opening at the upper end, the bottom plate of the liquid storage cavity seat is provided with the silicon chip placing column, the upper end surface of the silicon chip placing column is provided with the silicon chip, the two sides of the lower end part of the liquid storage cavity seat are respectively fixed with the liquid inlet pipe and the liquid outlet pipe, the upper end surface of the liquid storage cavity seat is provided with the pressure plate, the liquid storage cavity seat is tightly fixed.
Preferably, a base recess is formed in the upper surface of the base, and the lower end of the liquid storage cavity seat is embedded into the base recess.
Preferably, the liquid storage cavity seat, the liquid inlet pipe and the liquid outlet pipe are integrally made of glass materials or quartz materials.
Preferably, the silicon wafer placing column is made of a glass material or a quartz material.
Preferably, the upper end surface of the silicon wafer placing column is horizontally arranged.
Preferably, the plane of the transparent cover plate and the plane of the silicon wafer are both horizontally arranged.
The invention can well observe the activity of microorganisms and prevent the interference of external factors, and has simple structure and convenient use.
Drawings
Fig. 1 is a front view of the present invention.
Fig. 2 is a left side view of the present invention.
Fig. 3 is a top view of the present invention.
Fig. 4 is a sectional view a-a of fig. 3.
Fig. 5 is a perspective view of the present invention.
Detailed Description
The present invention will be further described with reference to the following specific examples.
A fluid observation vessel for observing microbial activity comprises a base 1, a liquid storage cavity base 2, a liquid inlet pipe 3, a liquid outlet pipe 4, a pressing plate 5, a fastening bolt 6, a transparent cover plate 7, a silicon wafer placing column 8, a silicon wafer 9 and a sealing ring 10; be equipped with stock solution chamber seat 2 at the upper surface of base 1, be equipped with in stock solution chamber seat 2 and be upper end open-ended stock solution chamber, it places post 8 to be equipped with the silicon chip on the bottom plate of stock solution chamber seat 2, the up end that post 8 was placed to the silicon chip is fixed with silicon chip 9, lower tip both sides at stock solution chamber seat 2 are fixed with feed liquor pipe 3 and drain pipe 4 respectively, be equipped with clamp plate 5 at the up end of stock solution chamber seat 2, stock solution chamber seat 2 compresses tightly through clamp plate 5 and fastening bolt 6 and fixes the upper surface at base 1, seal by sealing washer 10 between clamp plate 5 and the stock solution chamber seat 2, corresponding be fixed with transparent cover plate 7 on the clamp plate 5 of position directly over post 8 is placed to the silicon chip, transparent cover plate 7 is parallel arrangement with silicon chip 9.
The upper surface of base 1 is equipped with base sunken 1.1, the lower extreme embedding of stock solution chamber seat 2 is in this base sunken 1.1, can make stock solution chamber seat 2 install more firmly on base 1 like this.
The liquid storage cavity base 2, the liquid inlet pipe 3 and the liquid outlet pipe 4 are integrally made of glass materials or quartz materials.
The silicon wafer placing column 8 is made of a glass material or a quartz material.
The upper end surface of the silicon wafer placing column 8 is horizontally arranged, and the plane of the transparent cover plate 7 and the plane of the silicon wafer 9 are both horizontally arranged.
When the liquid-storing microscope is used, liquid is injected from the liquid inlet pipe 3 and submerges the silicon wafer 9 (namely, a two-dimensional material) placed on the silicon wafer placing column 8 so as to clear air in the liquid storing cavity seat 2, and the microscope observes the activity change of microorganisms on the silicon wafer 9 through the transparent cover plate 7. According to the invention, air and reaction liquid are isolated through the O-shaped sealing ring 10 and the pressing plate 5, so that the influence of air on an experiment is prevented. By injecting different fluids into the liquid inlet pipe 3, the change of the microbial activity on the surface of the silicon wafer 9 in the different fluids can be observed. The invention can observe the activity of microorganism well and prevent the interference of external factors.
Claims (6)
1. A fluid observation vessel for observing microbial activity comprises a base (1), a liquid storage cavity seat (2), a liquid inlet pipe (3), a liquid outlet pipe (4), a pressure plate (5), a fastening bolt (6), a transparent cover plate (7), a silicon wafer placing column (8), a silicon wafer (9) and a sealing ring (10); the method is characterized in that: the upper surface of the base (1) is provided with a liquid storage cavity seat (2), a liquid storage cavity with an opening at the upper end is arranged in the liquid storage cavity seat (2), a silicon wafer placing column (8) is arranged on the bottom plate of the liquid storage cavity seat (2), a silicon wafer (9) is arranged on the upper end surface of the silicon wafer placing column (8), a liquid inlet pipe (3) and a liquid outlet pipe (4) are respectively fixed on two sides of the lower end part of the liquid storage cavity seat (2), a pressure plate (5) is arranged on the upper end surface of the liquid storage cavity seat (2), the liquid storage cavity seat (2) is tightly pressed and fixed on the upper surface of the base (1) through the pressure plate (5) and a fastening bolt (6), a sealing ring (10) is used for sealing between the pressure plate (5) and the liquid storage cavity seat (2), a transparent cover plate (7) is fixed on the pressure plate (5) which corresponds to the position right above the silicon wafer placing column (8), and the transparent cover plate (7) and the silicon wafer (9) are arranged in parallel.
2. A fluid observation vessel for observing microbial activity according to claim 1, wherein: the upper surface of the base (1) is provided with a base recess (1.1), and the lower end of the liquid storage cavity seat (2) is embedded into the base recess (1.1).
3. A fluid observation vessel for observing microbial activity according to claim 1, wherein: the liquid storage cavity seat (2), the liquid inlet pipe (3) and the liquid outlet pipe (4) are integrally made of glass materials or quartz materials.
4. A fluid observation vessel for observing microbial activity according to claim 1, wherein: the silicon chip placing column (8) is made of glass materials or quartz materials.
5. A fluid observation vessel for observing microbial activity according to claim 1, wherein: the upper end surface of the silicon wafer placing column (8) is horizontally arranged.
6. A fluid observation vessel for observing microbial activity according to claim 1, wherein: the plane of the transparent cover plate (7) and the plane of the silicon chip (9) are both horizontally arranged.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011355474.XA CN112430521A (en) | 2020-11-27 | 2020-11-27 | Fluid observation vessel for observing microbial activity |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011355474.XA CN112430521A (en) | 2020-11-27 | 2020-11-27 | Fluid observation vessel for observing microbial activity |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112430521A true CN112430521A (en) | 2021-03-02 |
Family
ID=74698995
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011355474.XA Pending CN112430521A (en) | 2020-11-27 | 2020-11-27 | Fluid observation vessel for observing microbial activity |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112430521A (en) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101643702A (en) * | 2008-08-09 | 2010-02-10 | 清华大学 | Cell orientational culture system, cell orientational culture substrate and cell orientational culture method |
| CN101857837A (en) * | 2010-07-02 | 2010-10-13 | 上海交通大学 | Cell co-culture flow chamber |
| CN202139242U (en) * | 2011-07-05 | 2012-02-08 | 复旦大学 | Rotary fixed micro parallel plate flow chamber |
| WO2013140822A1 (en) * | 2012-03-23 | 2013-09-26 | 国立大学法人名古屋大学 | Cell culture base material and cell culture method |
| CN105670933A (en) * | 2016-02-22 | 2016-06-15 | 郭菲 | Parallel plate test chamber device for loading flow shear stress in cell culture |
| CN205635639U (en) * | 2016-04-11 | 2016-10-12 | 上海交通大学医学院附属第九人民医院 | Cell force means based on hydrodynamic shear is amazing |
| CN109468220A (en) * | 2018-11-15 | 2019-03-15 | 江南大学 | Graphene chip for microbial cell biological information real-time detection |
| US20190284615A1 (en) * | 2018-01-29 | 2019-09-19 | Rite Taste, LLC | Methods and devices for detection of pathogens |
| CN214300078U (en) * | 2020-11-27 | 2021-09-28 | 无锡费曼科技有限公司 | Fluid observation vessel for observing microbial activity |
-
2020
- 2020-11-27 CN CN202011355474.XA patent/CN112430521A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101643702A (en) * | 2008-08-09 | 2010-02-10 | 清华大学 | Cell orientational culture system, cell orientational culture substrate and cell orientational culture method |
| CN101857837A (en) * | 2010-07-02 | 2010-10-13 | 上海交通大学 | Cell co-culture flow chamber |
| CN202139242U (en) * | 2011-07-05 | 2012-02-08 | 复旦大学 | Rotary fixed micro parallel plate flow chamber |
| WO2013140822A1 (en) * | 2012-03-23 | 2013-09-26 | 国立大学法人名古屋大学 | Cell culture base material and cell culture method |
| CN105670933A (en) * | 2016-02-22 | 2016-06-15 | 郭菲 | Parallel plate test chamber device for loading flow shear stress in cell culture |
| CN205635639U (en) * | 2016-04-11 | 2016-10-12 | 上海交通大学医学院附属第九人民医院 | Cell force means based on hydrodynamic shear is amazing |
| US20190284615A1 (en) * | 2018-01-29 | 2019-09-19 | Rite Taste, LLC | Methods and devices for detection of pathogens |
| CN109468220A (en) * | 2018-11-15 | 2019-03-15 | 江南大学 | Graphene chip for microbial cell biological information real-time detection |
| CN214300078U (en) * | 2020-11-27 | 2021-09-28 | 无锡费曼科技有限公司 | Fluid observation vessel for observing microbial activity |
Non-Patent Citations (1)
| Title |
|---|
| 杨程、刘忠范: "《石墨烯复合材料》", 31 May 2020, 华东理工大学出版社, pages: 226 * |
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