CN213854612U - Liquid inlet device of chip - Google Patents
Liquid inlet device of chip Download PDFInfo
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- CN213854612U CN213854612U CN202022567717.8U CN202022567717U CN213854612U CN 213854612 U CN213854612 U CN 213854612U CN 202022567717 U CN202022567717 U CN 202022567717U CN 213854612 U CN213854612 U CN 213854612U
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Abstract
The utility model relates to a chip processing technology field discloses a feed liquor device of chip. The liquid inlet device of the chip is used for injecting a reagent into a gap of the chip, the chip comprises a transparent conductive cover with a sample inlet, the liquid inlet device of the chip comprises a flow guide block and a kit, the flow guide block is installed on the sample inlet, the flow guide block is provided with a flow guide hole, the first end of the flow guide hole is communicated with the gap of the chip, the bottom of the flow guide block is provided with a base plate, the base plate is arranged in the gap of the chip, the base plate is provided with an opening, and the opening is configured for guiding the reagent; the reagent box is used for storing reagent, the reagent box can be arranged on the flow guide block, the second end of the flow guide hole is communicated with the cavity of the reagent box, and the reagent is injected into the gap of the chip through the flow guide hole. The utility model discloses a reagent is pre-buried on digital micro-fluidic chip, has improved production efficiency to and realized the accurate control of reagent flow direction, avoided the waste of reagent, improve the chip quality.
Description
Technical Field
The utility model relates to a chip processing technology field especially relates to a feed liquor device of chip.
Background
At present, a digital microfluidic chip needs to be used in cooperation with a detection reagent to have a certain specific detection function, and mainly comprises an electrode array and a transparent conductive cover, wherein a hydrophobic layer and a dielectric layer are arranged on the upper surface of the electrode array, the transparent conductive cover (such as ITO glass) is arranged above the electrode array at intervals, and a gap for filling the reagent is formed between the transparent conductive cover and the hydrophobic layer.
At present, a transparent conductive cover is provided with a sample inlet, and a certain amount of liquid reagent sample is usually absorbed by a pipette and then aligned with the sample inlet, so that the reagent is completely injected into a gap between the transparent conductive cover and an electrode array on the premise of not contacting the surface of the electrode array. However, the use of a pipette to inject a reagent increases the use cost and has strong dependence on the reagent, and the traditional sample injection method cannot realize the pre-embedding of the detection reagent on the digital microfluidic chip, thereby greatly limiting the use environment and sample injection conditions of the digital microfluidic chip. In addition, in the traditional method, a liquid-transferring gun is used for sample adding, and due to the fact that a structure for guiding the flow of the reagent in the gap is lack, the reagent flows to an invalid area of the chip easily, so that the waste of the reagent or the sample is caused, and the product quality is poor.
Therefore, a liquid inlet device for a chip is needed to solve the above problems.
SUMMERY OF THE UTILITY MODEL
Based on above, an object of the utility model is to provide a feed liquor device of chip has realized that reagent is pre-buried on digital micro-fluidic chip, has improved production efficiency to and realized the accurate control of reagent flow direction, avoided the waste of reagent, improve the chip quality.
In order to achieve the purpose, the utility model adopts the following technical proposal:
an inlet device for a chip for injecting a reagent into a gap of the chip, the chip including a transparent conductive cover having a sample inlet, the inlet device comprising:
the flow guide block is arranged on the sample inlet and provided with a flow guide hole, the first end of the flow guide hole is communicated with the gap of the chip, the bottom of the flow guide block is provided with a base plate, the base plate is arranged in the gap of the chip, the base plate is provided with an opening, and the opening is configured to guide the flow of the reagent;
the reagent box is used for storing the reagent, the reagent box can be arranged on the flow guide block, the second end of the flow guide hole is communicated with the cavity of the reagent box, and the reagent is injected into the gap of the chip through the flow guide hole.
As an optimal technical scheme of the inlet means of chip, the bottom of kit is provided with first sealing film, the water conservancy diversion piece can puncture first sealing film, so that the kit install in on the water conservancy diversion piece, and make the water conservancy diversion hole communicate in the cavity of kit.
As a preferred technical scheme of the inlet means of chip, the casing of kit is flexible casing, when pressing the casing, the reagent through the water conservancy diversion hole is injected in the clearance of chip.
As a preferred technical scheme of the liquid inlet device of the chip, the shell of the kit is made of plastic or rubber.
As an inlet means's of chip preferred technical scheme, the top of kit is provided with the second sealing membrane, tears the second sealing membrane, so that the reagent warp the water conservancy diversion hole inject in the clearance of chip.
As a preferred technical scheme of the liquid inlet device of the chip, the top end face of the flow guide block is obliquely arranged.
As a preferred technical scheme of the liquid inlet device of the chip, the cross section of the top end of the flow guide block is in an inverted V-shaped sharp angle shape.
As an optimal technical scheme of the liquid inlet device of the chip, the flow guide blocks are integrally formed and are connected with the base plate in an interconnecting mode.
As a preferable technical solution of the liquid inlet device for the chip, the thickness of the pad is the same as the size of the gap of the chip.
As a preferred technical scheme of the liquid inlet device of the chip, the base plate is U-shaped.
The utility model has the advantages that:
the flow guide block is arranged at the sample inlet of the transparent conductive cover, so that the first end of the flow guide hole in the flow guide block is communicated with the gap of the chip, the reagent box is stored with reagent, the reagent box is arranged on the flow guide block, meanwhile, the second end of the flow guide hole is communicated with the cavity of the reagent box, and the reagent is injected into the gap of the chip through the flow guide hole. The utility model discloses a more convenient, low-cost, accomplish the feed liquor operation of reagent not restricted by environmental condition, predetermine in the reagent box moreover and can fill in advance reagent, realize that reagent is pre-buried on digital micro-fluidic chip, improved production efficiency. Moreover the bottom of water conservancy diversion piece is provided with the backing plate, and the backing plate is located the clearance of chip, is provided with the opening that is used for the reagent water conservancy diversion on the backing plate, has realized the effective area in guide reagent flow direction chip clearance, and the waste of reagent has been avoided to the accurate control of reagent flow direction, optimizes the encapsulation process flow, reduces manufacturing cost, has improved chip quality simultaneously.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings required to be used in the description of the embodiments of the present invention will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the contents of the embodiments of the present invention and the drawings without creative efforts.
FIG. 1 is a cross-sectional view of a liquid inlet device of a chip according to an embodiment of the present invention;
fig. 2 is a schematic structural diagram of a flow guide block of a chip according to an embodiment of the present invention;
fig. 3 is a schematic structural diagram of a liquid inlet device of a chip according to an embodiment of the present invention;
FIG. 4 is a schematic structural view of a kit provided in other embodiments;
fig. 5 is a schematic structural view of a plurality of guide blocks integrally formed according to an embodiment of the present invention.
The figures are labeled as follows:
1. a chip; 11. a transparent conductive cover; 12. a hydrophobic layer; 13. a dielectric layer; 2. a flow guide block; 21. a flow guide hole; 22. a base plate; 3. a kit; 31. a first sealing film; 32. and a second sealing film.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings and examples. It is to be understood that the specific embodiments described herein are merely illustrative of the invention and are not limiting of the invention. It should be further noted that, for the convenience of description, only some of the structures related to the present invention are shown in the drawings, not all of the structures.
In the description of the present invention, unless expressly stated or limited otherwise, the terms "connected," "connected," and "fixed" are to be construed broadly, e.g., as meaning permanently connected, detachably connected, or integral to one another; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.
In the present disclosure, unless expressly stated or limited otherwise, the first feature "on" or "under" the second feature may comprise direct contact between the first and second features, or may comprise contact between the first and second features not directly. Also, the first feature being "on," "above" and "over" the second feature includes the first feature being directly on and obliquely above the second feature, or merely indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.
In the description of the present embodiment, the terms "upper", "lower", "left", "right", and the like are used in the orientation or positional relationship shown in the drawings only for convenience of description and simplicity of operation, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed in a specific orientation, and be operated, and thus should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used only for descriptive purposes and are not intended to have a special meaning.
At present, a transparent conductive cover is provided with a sample inlet, and a certain amount of liquid reagent sample is usually absorbed by a pipette and then aligned with the sample inlet, so that the reagent is completely injected into a gap between the transparent conductive cover and an electrode array on the premise of not contacting the surface of the electrode array. However, the use of a pipette to inject a reagent increases the use cost and has strong dependence on the reagent, and the traditional sample injection method cannot realize the pre-embedding of the detection reagent on the digital microfluidic chip, thereby greatly limiting the use environment and sample injection conditions of the digital microfluidic chip. In addition, in the traditional method, a liquid-transferring gun is used for sample adding, and due to the fact that a structure for guiding the flow of the reagent in the gap is lack, the reagent flows to an invalid area of the chip easily, so that the waste of the reagent or the sample is caused, and the product quality is poor.
In order to solve the above problems, as shown in fig. 1 to 5, the present embodiment provides a chip inlet device for injecting a reagent into a gap of a chip 1, the device including a flow guide block 2 and a reagent kit 3.
Specifically, the digital microfluidic chip 1 mainly includes an electrode array and a transparent conductive cover 11, wherein a hydrophobic layer 12 and a dielectric layer 13 are disposed on an upper surface of the electrode array, the transparent conductive cover 11 (for example, ITO glass) is disposed above the electrode array at an interval, and a gap for filling a reagent is formed between the transparent conductive cover 11 and the hydrophobic layer 12. The transparent conductive cover 11 is provided with a sample inlet, the flow guide block 2 is arranged on the sample inlet, the flow guide block 2 is provided with a flow guide hole 21, the first end of the flow guide hole 21 is communicated with the gap of the chip 1, the bottom of the flow guide block 2 is provided with a backing plate 22, the backing plate 22 is arranged in the gap of the chip 1, the backing plate 22 is provided with an opening, and the opening is configured to guide the flow of the reagent; the reagent box 3 is used for storing reagent, the reagent box 3 can be arranged on the flow guide block 2, the second end of the flow guide hole 21 is communicated with the cavity of the reagent box 3, and the reagent is injected into the gap of the chip 1 through the flow guide hole 21.
When injecting the reagent, the flow guide block 2 is firstly installed at the sample inlet of the transparent conductive cover 11, so that the first end of the flow guide hole 21 on the flow guide block 2 is communicated with the gap of the chip 1, the reagent is stored in the reagent box 3, and the reagent box 3 is installed on the flow guide block 2. Meanwhile, the second end of the diversion hole 21 is communicated with the chamber of the reagent kit 3, and the reagent is injected into the gap of the chip 1 through the diversion hole 21. The liquid inlet operation of the reagent is completed more conveniently and rapidly without being limited by environmental conditions at low cost, the reagent can be filled in advance by presetting the reagent in the kit 3, the embedding of the reagent on the digital microfluidic chip 1 is realized, and the production efficiency is improved. Moreover the bottom of water conservancy diversion piece 2 is provided with backing plate 22, and backing plate 22 is located chip 1's clearance, is provided with the opening that is used for the reagent water conservancy diversion on the backing plate 22, has realized the effective area in guide reagent flow direction chip 1 clearance, and the accurate control of reagent flow direction has avoided the waste of reagent, optimizes the encapsulation process flow, reduces manufacturing cost, has improved chip 1 quality simultaneously. Of course, in other embodiments, the reagent cartridge 3 may be replaced by a pipette or a sample injector, and may be used in combination with the flow guide block 2 to inject the reagent.
Preferably, the bottom of the reagent kit 3 is provided with a first sealing film 31, and the baffle block 2 can pierce the first sealing film 31, so that the reagent kit 3 is mounted on the baffle block 2, and the baffle hole 21 is communicated with the chamber of the reagent kit 3. In order to facilitate the diversion block 2 to easily puncture the first sealing film 31, in the present embodiment, as shown in fig. 1, the top end surface of the diversion block 2 is preferably disposed in an inclined manner to reduce the contact area of the top end surface to the first sealing film 31, so as to increase the acting force of the end surface to the first sealing film 31, which is beneficial to puncturing the first sealing film 31. Further preferably, the end surface of the top end of the baffle block 2 is provided with an inverted V-shaped sharp corner, and the contact area of the top end surface to the first sealing film 31 is also reduced, so as to increase the acting force of the end surface to the first sealing film 31, and facilitate to puncture the first sealing film 31.
After the reagent kit 3 is mounted on the flow guide block 2, although the flow guide holes 21 are communicated with the cavity of the reagent kit 3, the top of the reagent kit 3 is sealed, so that the reagent cannot smoothly flow into the gap through the flow guide holes 21 under the action of pressure, and the reagent in the cavity of the reagent kit 3 can smoothly flow into the gap of the chip 1. In the embodiment, it is pre-selected that the casing of the reagent cartridge 3 is a deformable casing, and when the casing is pressed, the casing is deformed to compress the space of the chamber of the reagent cartridge 3, and simultaneously, the reagent is injected into the gap of the chip 1 through the diversion hole 21. In this embodiment, the material of the housing of the reagent kit 3 is preferably plastic, which is favorable for processing and has low production cost. In other embodiments, the material can be deformable materials such as silica gel, on one hand, the silica gel is easy to deform, and the use requirement is met; on the other hand, the shape of the reagent cartridge 3 can be recovered after the silica gel is pressed, and the reagent cartridge 3 can be reused after the first sealing film 31 is replaced.
In other embodiments, as shown in fig. 4, a second sealing film 32 may be disposed on the top of the reagent container 3, and the second sealing film 32 may seal the reagent container 3 when the injection is not needed; when injection is needed, the second sealing film 32 is torn, and the pressure of the upper part of the reagent in the cavity is increased, so that the reagent can smoothly flow into the gap.
Because the number of the sample inlets of the transparent conductive cover 11 on the chip 1 is large, the plurality of flow guide blocks 2 are integrally formed, and the plurality of flow guide blocks 2 are connected with each other through the backing plate 22. Each diversion hole 21 above the backing plate 22 corresponds to a diversion opening to realize diversion of the reagent. The integrated structure of the backing plate 22 is beneficial to the processing of the backing plate 22, is convenient for the assembly of the flow guide block 2 and reduces the processing cost. Further preferably, in this embodiment, the thickness of the pad 22 is the same as the size of the gap of the chip 1, the upper surface of the pad 22 is attached to the lower surface of the transparent conductive cover 11, the lower surface of the pad 22 is attached to the upper surface of the electrode array, the pad 22 not only has the function of guiding current, but also has the function of supporting the transparent conductive cover 11, so as to prevent the transparent conductive cover 11 from deforming during the pressing process, and the pad 22 also has the function of isolating the gap of the chip 1, so that the reagent is partitioned, and cross contamination is prevented. Of course, the deflector block 2 may have a split structure.
Further, the shape of the backing plate 22 in this embodiment is U-shaped. In other embodiments, the shape of the backing plate 22 can be tailored to the direction of reagent flow.
It should be noted that the foregoing is only a preferred embodiment of the present invention and the technical principles applied. It will be understood by those skilled in the art that the present invention is not limited to the particular embodiments described herein, but is capable of various obvious changes, rearrangements and substitutions as will now become apparent to those skilled in the art without departing from the scope of the invention. Therefore, although the present invention has been described in greater detail with reference to the above embodiments, the present invention is not limited to the above embodiments, and may include other equivalent embodiments without departing from the scope of the present invention.
Claims (10)
1. An inlet means for a chip for injecting a reagent into a gap of a chip (1), the chip (1) comprising a transparent conductive cover (11) having a sample inlet, the inlet means comprising:
the flow guide block (2) is installed on the sample inlet, a flow guide hole (21) is formed in the flow guide block (2), the first end of the flow guide hole (21) is communicated with the gap of the chip (1), a base plate (22) is arranged at the bottom of the flow guide block (2), the base plate (22) is arranged in the gap of the chip (1), an opening is formed in the base plate (22), and the opening is configured for guiding the flow of the reagent;
the reagent box (3) is used for storing the reagent, the reagent box (3) can be arranged on the flow guide block (2), the second end of the flow guide hole (21) is communicated with the cavity of the reagent box (3), and the reagent is injected into the gap of the chip (1) through the flow guide hole (21).
2. The inlet means for chips according to claim 1, characterized in that the bottom of the reagent box (3) is provided with a first sealing film (31), and the diversion block (2) can pierce through the first sealing film (31) to mount the reagent box (3) on the diversion block (2) and communicate the diversion hole (21) with the chamber of the reagent box (3).
3. The inlet device for chips according to claim 1, wherein the casing of the reagent cartridge (3) is a deformable casing, and when the casing is pressed, the reagent is injected into the gap of the chip (1) through the diversion hole (21).
4. The inlet device for chips according to claim 3, characterized in that the casing of the reagent kit (3) is made of plastic or rubber.
5. The inlet means for chips according to claim 1, characterized in that a second sealing film (32) is disposed on the top of the reagent box (3), and the second sealing film (32) is torn to inject the reagent into the gap of the chip (1) through the diversion hole (21).
6. The inlet means for chips according to claim 2, characterized in that the top end surface of the flow guide block (2) is arranged obliquely.
7. The inlet means for chips according to claim 2, characterized in that the top end of the deflector block (2) has a sharp angle shape with an inverted V shape in cross section.
8. The inlet means for chips according to claim 1, characterized in that a plurality of said flow guide blocks (2) are integrally formed, and a plurality of said flow guide blocks (2) are connected to each other by said backing plate (22).
9. The inlet means for chips according to claim 1, characterized in that the thickness of the pad (22) is the same as the size of the gap of the chip (1).
10. Inlet means for chips according to any of claims 1-9, characterized in that the shim plate (22) is U-shaped.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022567717.8U CN213854612U (en) | 2020-11-09 | 2020-11-09 | Liquid inlet device of chip |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022567717.8U CN213854612U (en) | 2020-11-09 | 2020-11-09 | Liquid inlet device of chip |
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| CN213854612U true CN213854612U (en) | 2021-08-03 |
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| CN202022567717.8U Active CN213854612U (en) | 2020-11-09 | 2020-11-09 | Liquid inlet device of chip |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023087777A1 (en) * | 2021-11-17 | 2023-05-25 | 江苏液滴逻辑生物技术有限公司 | System device and method for quantitatively injecting tested sample into chip, and use |
| WO2023151243A1 (en) * | 2022-02-08 | 2023-08-17 | 江苏液滴逻辑生物技术有限公司 | Lyosphere pre-embedding structure, digital microfluidic chip, and pre-embedding and liquid injection method |
-
2020
- 2020-11-09 CN CN202022567717.8U patent/CN213854612U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023087777A1 (en) * | 2021-11-17 | 2023-05-25 | 江苏液滴逻辑生物技术有限公司 | System device and method for quantitatively injecting tested sample into chip, and use |
| WO2023151243A1 (en) * | 2022-02-08 | 2023-08-17 | 江苏液滴逻辑生物技术有限公司 | Lyosphere pre-embedding structure, digital microfluidic chip, and pre-embedding and liquid injection method |
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Address after: 215300 Building 2, 1001 Yuyang Road, Yushan Town, Kunshan City, Suzhou City, Jiangsu Province Patentee after: Jiangsu drop logic Biotechnology Co.,Ltd. Address before: 215300 unit 308, biological building, 168 Yuanfeng Road, Kunshan high tech Zone, Suzhou City, Jiangsu Province Patentee before: Jiangsu Aosu Liquid Core Biotechnology Co.,Ltd. |
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