CN202139242U - Rotary fixed micro parallel plate flow chamber - Google Patents

Rotary fixed micro parallel plate flow chamber Download PDF

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Publication number
CN202139242U
CN202139242U CN 201120233601 CN201120233601U CN202139242U CN 202139242 U CN202139242 U CN 202139242U CN 201120233601 CN201120233601 CN 201120233601 CN 201120233601 U CN201120233601 U CN 201120233601U CN 202139242 U CN202139242 U CN 202139242U
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CN
China
Prior art keywords
silicagel pad
cell culture
teflon film
base
chamber
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Expired - Fee Related
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CN 201120233601
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Chinese (zh)
Inventor
徐建明
覃开蓉
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Fudan University
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Fudan University
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Priority to CN 201120233601 priority Critical patent/CN202139242U/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/50—Means for positioning or orientating the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/20—Material Coatings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements

Abstract

The utility model belongs to the technical field of biological mechanics experimental devices, in particular to a rotary fixed micro parallel plate flow chamber. The plate flow chamber adopts an integrated design of a cell culture chamber and a fixing and positioning device, and specifically consists of a liquid inlet, a liquid outlet, a flat cube cell culture chamber, an observation window, and the fixing and positioning device, wherein the flat cube cell culture chamber is formed by a transparent biocompatible circular plate inlet, a transparent biocompatible circular plate outlet, a Teflon thin film and cover glass. The fixing and positioning device is formed by a tightening cover, a pressure plate, a base, a silicone pad and a positioning groove. The rotary fixed micro parallel plate flow chamber can be used for biomechanics and mechanobiology experiments of vascular endothelial cells successfully.

Description

A kind of rotational fixation type parallel flat flow chamber that declines
Technical field
The utility model belongs to biomechanics experiment device technique field, is specifically related to a kind of rotational fixation type parallel flat flow chamber that declines.
Background technology
The research of cell micro-environment and cell interaction becomes the cytobiology hot research fields day by day.The hydromeehanics and the biological chemical environment that make up cell stripped (in vitro) growth are the prerequisites of research hydromeehanics and biological chemical environment and cell interaction.Height is one of main means that make up stream of cells mechanics and biological chemical environment much smaller than horizontal and vertical geometrical dimension and size at the parallel flat flow chamber of micron, millimeter or centimetre magnitude, has particularly obtained in cell biological mechanics and the research work of power biology widely-used in biomechanics.Yet the normally used parallel flat flow chamber of people is fixed by a plurality of screws, and the installation and removal process is complicated, causes defectives such as lengthening experimental period, the easy damaged of cell.Press for a kind of attaching/detaching process convenient, reduce cell injury, and novel parallel flat flow chamber device that can online observation as far as possible.
Summary of the invention
The purpose of the utility model is to provide a kind of cyto-mechanics experimental installation, adopts the integral structure of rotational fixation and cell culture chamber, accelerates the dismounting and the installation process of flow cavity, thus shortening cyto-mechanics experimental period; Adopt exquisite interior locating device, prevent the damage of installation process pair cell; Adopt transparent biocompatibility circular dull and stereotyped, be convenient to online observation.
As shown in Figure 1, rotational fixation miniature parallel flat flow chamber by: by pressing plate 2, screw and cover 4, base 5, silicagel pad 6, viewing window 7, deckglass 8, cell culture chamber 9, silicagel pad 10, Teflon film 12, circular dull and stereotyped 14 constitute; Wherein base 5 cover the circular external form of 4 tools with screwing, and is identical and screw through screw thread between the two; Be provided with the viewing window 7 of rectangular hollow structure in the middle of the base plate of base 5; Silicagel pad 6 has the rectangular hollow structure identical with viewing window 7; This rectangle silicagel pad 6 is arranged in the step on viewing window 7 tops; Deckglass 8 is positioned on the silicagel pad 6, and Teflon film 12 has same rectangular hollow structure, and this rectangle Teflon film 12 is positioned on the deckglass 8; Circular dull and stereotyped 14 are positioned on the Teflon film 12, and top has import 1 and outlet 3, placement silicagel pad 10 above the circle dull and stereotyped 14, placement pressing plates 2 above the silicagel pad 10; Deckglass 8, Teflon film 12 and circular dull and stereotyped 14 constitute a flat cubic chamber, are cell culture chamber 9, and it highly is the thickness of Teflon film, and length and width then are the length and the width of Teflon film hollow, rectangular; Circular dull and stereotyped 14 2 sides have the recessed locating slot of convex part and base 5 to coincide, and play fixed cell culture chamber 9.
The silicagel pad 10 that place pressing plate 2 bottoms and the silicagel pad 6 of base 5 tops placement; Play balanced seal cell culture chamber 9; Just cover 4 when screwing and entangle all parts of intermediary and when screwing through screw thread with base 5, silicagel pad 10 and silicagel pad 6 play the effect of balanced seal cell culture chamber 9.
As shown in Figure 3, cell cultures is on the deckglass of bottom.When nutrient solution flows into cell culture chamber and when outlet is flowed out, because the viscous friction meeting pair cell between motion liquid and the cell produces the effect of fluid shearing stress from inlet; Simultaneously if in motion liquid, add the various biological chemistry factors, then can pair cell produce the hormesis of the biological chemistry factor.Than a plurality of screw fixed of the usefulness parallel flat flow chamber device that people use always, the utility model patent adopts screw lid, base, silicagel pad and locating slot of screw thread to constitute stationary installation, makes the dismounting of flow cavity and assembling process become simple and easy.The particularly design of locating slot makes screw thread screw when rotation lid can keep deckglass motionless, thereby avoids or reduce the cell injury on the deckglass.
The utility model can successfully be used for the biomechanics and power biology (mechanobiology) experiment of vascular endothelial cell.
Description of drawings
Fig. 1 rotational fixation type parallel flat flow chamber structure iron that declines.
Cell culture chamber amplification assumption diagram among Fig. 2 Fig. 1.
Fig. 3 cell culture chamber synoptic diagram.
Fig. 4 experimental installation synoptic diagram.
Label among the figure: 1-import, 2-pressing plate, 3-outlet, the 4-lid of screwing, 5-base, 6-silicagel pad, 7-viewing window, 8-deckglass, 9-cell culture chamber, 10-silicagel pad, 11-locating slot, 12-Teflon film, 13-locating slot, the circular flat board of 14-.
Embodiment
As depicted in figs. 1 and 2, the utility model mainly is made up of liquid-inlet 1, outlet 3, flat cubes cell cultures chamber 9, observation window 7, fixing and locating device integrated design system.Wherein flat cubes cell cultures chamber 9 is made up of the circular flat board of transparent biocompatibility, Teflon film 12 and deckglass 8; Fixing and locating device cover 4 by screwing, base 5, silicagel pad 6 and 10 and locating slot 11 and 13 constitute.
In the present embodiment; Whole flow cavity profile diameter 82mm, height of contour 35mm, core component cell cultures cavity length 44mm; Width 20 mm; Height 0.1～1mm, circular dull and stereotyped import and export of transparent biocompatibility is made up of polycarbonate plate, can constitute cyto-mechanics loading system (Fig. 4) with reservoir, peristaltic pump (or jerk pump) and silicone tube.The utility model patent successfully is applied to the intracellular calcium dynamic response experiment of vascular endothelial cell under the effect of fluid shearing stress.

Claims (1)

1. rotational fixation type parallel flat flow chamber that declines is characterized in that by pressing plate (2) lid (4) of screwing; Base (5), silicagel pad (6), viewing window (7); Deckglass (8), cell culture chamber (9), silicagel pad (10); Teflon film (12), circular dull and stereotyped (14) constitute; Wherein, base (5) and the circular external form of lid (4) tool of screwing are coincide through screw thread between the two and are screwed; Be provided with the viewing window (7) of rectangular hollow structure in the middle of the base plate of base (5); Silicagel pad (6) has and the identical rectangular hollow structure of viewing window (7); This rectangle silicagel pad (6) is arranged in the step on viewing window (7) top; Deckglass (8) is positioned on the silicagel pad (6), and Teflon film (12) has same rectangular hollow structure, and this rectangle Teflon film (12) is positioned on the deckglass (8); Circular dull and stereotyped (14) are positioned on the Teflon film (12); And top has import (1) and outlet (3), places silicagel pad (10) above circular dull and stereotyped (14), places pressing plate (2) above the silicagel pad (10); Deckglass (8), Teflon film (12) and flat cubic chamber of circular dull and stereotyped (14) formation; Be cell culture chamber (9); It highly is the thickness of Teflon film, and length and width then are the length and the width of Teflon film hollow, rectangular; Two sides of circular dull and stereotyped (14) have the recessed locating slot of convex part and base (5) to coincide, and play fixed cell culture chamber (9).
CN 201120233601 2011-07-05 2011-07-05 Rotary fixed micro parallel plate flow chamber Expired - Fee Related CN202139242U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 201120233601 CN202139242U (en) 2011-07-05 2011-07-05 Rotary fixed micro parallel plate flow chamber

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Application Number Priority Date Filing Date Title
CN 201120233601 CN202139242U (en) 2011-07-05 2011-07-05 Rotary fixed micro parallel plate flow chamber

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CN202139242U true CN202139242U (en) 2012-02-08

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105154316A (en) * 2015-09-30 2015-12-16 重庆大学 Blood vessel-like running cavity
CN105176814A (en) * 2015-09-30 2015-12-23 重庆大学 Use method of blood vessel simulating flow chamber
CN105296326A (en) * 2015-09-30 2016-02-03 重庆大学 Vascular fluid platform with independent chamber
CN105483004A (en) * 2016-02-22 2016-04-13 上海泉众机电科技有限公司 Parallel plate experiment cavity device
WO2017015123A1 (en) * 2015-07-17 2017-01-26 Boyd Lawrence M Apparatus and method for sorting of cells
CN109153019A (en) * 2016-05-18 2019-01-04 米兰综合工科大学 device for cell culture
CN112430521A (en) * 2020-11-27 2021-03-02 无锡费曼科技有限公司 Fluid observation vessel for observing microbial activity

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017015123A1 (en) * 2015-07-17 2017-01-26 Boyd Lawrence M Apparatus and method for sorting of cells
CN105154316A (en) * 2015-09-30 2015-12-16 重庆大学 Blood vessel-like running cavity
CN105176814A (en) * 2015-09-30 2015-12-23 重庆大学 Use method of blood vessel simulating flow chamber
CN105296326A (en) * 2015-09-30 2016-02-03 重庆大学 Vascular fluid platform with independent chamber
CN105154316B (en) * 2015-09-30 2017-04-12 重庆大学 Blood vessel-like running cavity
CN105176814B (en) * 2015-09-30 2017-09-12 重庆大学 A kind of imitative blood flow chamber application method
CN105483004A (en) * 2016-02-22 2016-04-13 上海泉众机电科技有限公司 Parallel plate experiment cavity device
CN105483004B (en) * 2016-02-22 2020-04-14 上海泉众机电科技有限公司 Parallel flat plate experimental cavity device
CN109153019A (en) * 2016-05-18 2019-01-04 米兰综合工科大学 device for cell culture
CN109153019B (en) * 2016-05-18 2021-09-24 米兰综合工科大学 Device for cell culture
CN112430521A (en) * 2020-11-27 2021-03-02 无锡费曼科技有限公司 Fluid observation vessel for observing microbial activity

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GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20120208

Termination date: 20140705

EXPY Termination of patent right or utility model