CN210340942U - Cell culture device for applying mechanical stimulation by simulating blood vessel pulsation - Google Patents
Cell culture device for applying mechanical stimulation by simulating blood vessel pulsation Download PDFInfo
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- CN210340942U CN210340942U CN201921332409.8U CN201921332409U CN210340942U CN 210340942 U CN210340942 U CN 210340942U CN 201921332409 U CN201921332409 U CN 201921332409U CN 210340942 U CN210340942 U CN 210340942U
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Abstract
The utility model belongs to the technical field of cell culture, in particular to a cell culture device which applies mechanical stimulation by simulating blood vessel pulsation, comprising a power system, wherein the power system is connected with a culture system through a hose to form a connecting loop; the culture system comprises a pulsation groove and a culture rack in interference fit with the pulsation groove; a culture tank is arranged on the pulsation tank, and a water tank matched with the culture tank is arranged on the bottom surface of the culture tank; the water tank is provided with a high-elasticity film matched with the water tank, and the high-elasticity film is adhered with a fiber film matched with the high-elasticity film; the high-elasticity film and the fiber film are matched with the culture shelf. The device can achieve the effect of soft mechanical stimulation by simulating the pulsation of blood vessels by matching the power system with the culture system, applies acting force by a peristaltic pump in the pressurizing process, and achieves softer mechanical stimulation by combining a high-elasticity film and a fiber film through the matching between the pulsation groove and the culture frame.
Description
Technical Field
The utility model belongs to the technical field of cell culture, thereby concretely relates to exert mechanical stimulation's cell culture device through simulation blood vessel pulsation.
Background
Under physiological conditions, cells perform vital activities in a complex dynamic environment. Cells are subject to mechanical stimuli during growth, such as blood shear stress, tensile force, pressure, pulsation, etc. However, most of the cell culture experiments involving the research in cell biology, molecular biology, genetics and immunology still widely use culture dishes limited to a single static culture environment. Obviously, the results of the test of cells cultured in a static environment are very different from those in a dynamic environment. Therefore, the situation of cell proliferation can be reflected more truly by establishing a dynamic survival microenvironment simulating the physiological environment in vivo. The existing in vitro cell culture technology generally places a culture dish in an incubator, takes out the culture dish after culturing for a period of time, mechanically stimulates the culture dish and observes the feedback of cells, is inconvenient to operate and low in efficiency, has low culture quality, and cannot provide good reference for the research of cell behaviors.
SUMMERY OF THE UTILITY MODEL
In order to overcome the inhomogeneous and uncontrollable problem of dynamics of present gaseous pressurization in-process cell atress, the utility model provides a thereby the amazing cell culture device of mechanics is exerted through simulation vascular pulsation that the mechanics is amazing softer, controllability is strong and easy operation is convenient.
Based on the above-mentioned purpose, the utility model discloses a following technical scheme realizes:
a cell culture device for applying mechanical stimulation by simulating blood vessel pulsation comprises a power system, wherein the power system is connected with a culture system through a hose to form a connecting loop; the culture system comprises a pulsation groove and a culture rack in interference fit with the pulsation groove; a culture tank is arranged on the pulsation tank, and a water tank matched with the culture tank is arranged on the bottom surface of the culture tank; the water tank is provided with a high-elasticity film matched with the water tank, and the high-elasticity film is provided with a fiber film tightly matched with the high-elasticity film; the high-elasticity film and the fiber film are matched with the culture shelf.
Preferably, two opposite side surfaces of the water tank are respectively provided with a strip-shaped groove which is in clearance fit with the side surface of the water tank, the other two side surfaces of the water tank are respectively provided with a water through hole, the outer side surface of any strip-shaped groove is the same as the inner surface of the culture tank, and the water through holes are connected with the hose through the pulsation groove.
Preferably, the water through holes are all arranged at the center of the side surface of the water tank, and the diameters of the water through holes are 1/2-5/6 of the depth of the water tank.
Preferably, the culture frame is provided with an opening which is axially symmetrical along the culture frame, the bottom surface of the culture frame is provided with a bulge which is in interference fit with the culture tank, the bulge is arranged along the perimeter of the opening, and the bulge is provided with a pair of strip-shaped bulges which are matched with the strip-shaped grooves; the top surface of the culture shelf is provided with a culture shelf cover matched with the opening.
Preferably, the side surfaces of the culture shelf protrude out of the side surfaces of the pulsating groove.
Preferably, the power system comprises a peristaltic pump and a filter bottle connected with the peristaltic pump through a hose, and the filter bottle is connected with the limber through a hose.
Preferably, the diameter of the water passage hole is equal to the diameter of the hose; the culture shelf cover is a transparent cover plate; the hose is a silica gel hose.
Compared with the prior art, the beneficial effects of the utility model are as follows:
(1) the power system achieves the effect of flexibly applying mechanical stimulation by simulating the pulsation of blood vessels through the cooperation of the hose and the culture system, the pulsation groove and the culture frame in interference fit can achieve stable movable connection between the pulsation groove and the culture frame, the water tank and the high-elasticity film are matched to achieve flexible stimulation on cells cultured on the fiber film, the high-elasticity film and the fiber film are matched with the culture frame to achieve fixing and stable work of the fixing frame on the two layers of films, and the high-elasticity film and the fiber film can be conveniently replaced.
(2) The water through holes are matched with the hose to realize the water flow entering and flowing out of the water tank, and the strip-shaped grooves play a role in fixing the high-elasticity film and the fiber film.
(3) The limbers are all arranged at the center of the side surface of the water tank, so that the water in the water tank can be changed along with the change of the water flow to the maximum extent, and the timely reaction to the high-elasticity film is realized. The diameter of the limber hole is half of the depth of the water channel at the minimum and 5/6 of the depth of the water channel at the maximum, so that the high-elasticity film can be ensured to react to the water flow change in time.
(4) The opening is matched with the culture frame cover, so that the environmental pollution in the culture dish and the evaporation of culture solution can be prevented, the cell culture state can be observed in real time, and the growth condition of cell culture can be analyzed. The bulges in interference fit with the culture tank play a role in tight fit of the culture rack and the pulsation tank, and the pulsation tank and the culture rack can be connected together by exerting force. The strip-shaped bulges are matched with the strip-shaped grooves, so that the function of fixing the high-elasticity film and the fiber film is realized, and the function of fixing the culture rack and the pulsating groove is also realized.
(5) The side surfaces of the culture rack protrude out of the side surfaces of the pulsation grooves, so that the culture rack and the pulsation grooves can be conveniently separated under stress.
(6) The peristaltic pump has the characteristics of no pollution, high precision, good sealing performance, simple maintenance and the like, a loop is formed among the peristaltic pump, the filter bottle and the pulsation groove, the effect of recycling is realized, and the filter bottle is used for discharging air in the hose so as to ensure the stable change of water pressure; the periodic water supply of the peristaltic pump enables the water pressure in the pulsation groove to be changed continuously, the changed water pressure drives the high-elasticity film covered above the water groove to deform, the high-elasticity film drives the fiber film tightly attached to the high-elasticity film to vibrate in a fluctuating mode, and then force stimulation is transmitted to cells cultured on the fiber film.
(7) The diameter of the limber hole is equal to that of the hose, so that excessive or insufficient water flow gathering in the water tank caused by different diameters of the limber hole and the hose can be avoided, and the phenomenon that a high-elasticity film on the water tank cannot accurately reflect the continuous change of water pressure is avoided. The culture frame cover is set to be a transparent cover plate, so that the environment pollution in the culture dish and the evaporation of culture solution can be prevented, the cell culture state can be observed in real time, and the growth condition of cell culture is analyzed. The hose is a silica gel hose and has the advantages of softness, high temperature resistance, stable performance and the like.
To sum up, the utility model discloses a driving system and culture system cooperate and realize reaching softly applying the amazing effect of mechanics through simulation blood vessel pulsation, the device applys the effort through the peristaltic pump in pressurization process, through the cooperation between pulsation groove and the culture rack, through combining high elasticity film, fibrous film realizes softer mechanics amazing, can guarantee to obtain more accurate experimental result under the condition of cell normal growth, and it is fixed through interference fit between pulsation groove and the culture rack, only need can open the device installation hard or take off fibrous film, it is more convenient to operate, the culture rack lid is established to the clear cover plate and not only can prevent culture dish internal environment pollution and culture solution evaporation, can also the real-time observation cell culture state, the growth condition of analysis cell culture.
Drawings
FIG. 1 is a schematic connection diagram of embodiment 1;
FIG. 2 is a schematic view of the structure of a pulsating tank and a culture rack in example 1;
FIG. 3 is a schematic view of a pulsating groove structure of embodiment 1;
FIG. 4 is a schematic view of the structure of a culture shelf of example 1;
FIG. 5 is a schematic view of the structure of a culture shelf in example 1;
FIG. 6 is a schematic view of the structure of a pulsating tank and a culture rack of example 2;
FIG. 7 is a schematic view of a pulsating groove structure of embodiment 2;
FIG. 8 is a schematic view of the structure of a culture shelf in example 2.
In the figure, 1, a peristaltic pump, 2, a hose, 3, a filter flask, 4, a culture system, 5, a pulsation groove, 6, a culture shelf, 7, a fiber film, 8, a high-elasticity film, 9, a culture shelf cover, 10, a water tank, 11, a water through hole, 12, a strip-shaped groove, 13, a culture groove, 14, an opening, 15, a bulge, 16 and a strip-shaped bulge.
Detailed Description
The present invention will be described in further detail with reference to the following specific examples, which are not intended to limit the scope of the present invention.
Example 1:
a cell culture device for applying mechanical stimulation by simulating blood vessel pulsation is structurally shown in figures 1-5 and comprises a power system, wherein the power system comprises a peristaltic pump 1, the power system further comprises a filter bottle 3 connected with the peristaltic pump 1 through a hose 2, and the filter bottle 3 is connected with a water through hole 11 through the hose 2. The power system is connected with the culture system 4 through the hose 2 to form a connecting loop; the culture system 4 comprises a pulsation groove 5, and the culture system 4 further comprises a culture rack 6 in interference fit with the pulsation groove 5; a culture tank 13 is arranged on the pulsation tank 5, and a water tank 10 matched with the culture tank 13 is arranged on the bottom surface of the culture tank 13; the water tank 10 is provided with a high-elasticity film 8 matched with the water tank 10, and the high-elasticity film 8 is adhered with a fiber film 7 matched with the high-elasticity film 8; the high-elasticity film 8 and the fiber film 7 are matched with the culture shelf 6.
Two opposite side surfaces of the water tank 10 are respectively provided with a strip-shaped groove 12 which is in clearance fit with the side surface of the water tank 10, the other two side surfaces of the water tank 10 are respectively provided with a water through hole 11, the outer side surface of any strip-shaped groove 12 is the same as the inner surface of the culture tank 13, and the water through hole 11 is connected with the hose 2 through the pulsation groove 5. The water through holes 11 are all arranged at the center of the side surface of the water tank 10, and the diameters of the water through holes 11 are 1/2-5/6 of the depth of the water tank 10. The diameter of the water through hole 11 is equal to that of the hose 2; the culture shelf cover 9 is a transparent cover plate; the hose 2 is a silica gel hose.
The culture shelf 6 is provided with an opening 14 which is axially symmetrical along the culture shelf 6, the bottom surface of the culture shelf 6 is provided with a bulge 15 which is in interference fit with the culture tank 13, the bulge 15 is arranged along the perimeter of the opening 14, and the bulge 15 is provided with a pair of strip-shaped bulges 16 which are matched with the strip-shaped grooves 12; the top surface of the culture shelf 6 is provided with a culture shelf cover 9 which is matched with the opening 14. The side surfaces of the culture shelf 6 all protrude out of the side surface of the pulsation groove 5.
When in use, the first step is as follows: cleaning and disinfecting the device; the peristaltic pump 1 and the hose 2 are scrubbed by 95% alcohol, the high-elasticity film 8 and the fiber film 7 are soaked by 95% alcohol overnight, the filter bottle 3, the pulsation groove 5 and the culture rack 6 are sterilized at high temperature, and finally, all parts are irradiated by ultraviolet lamps. The second step is that: preparing a cell culture solution; and selecting a corresponding solution formula to prepare the cell culture solution aiming at the selected cells and the culture purpose in an aseptic environment. The third step: installing a high-elasticity film 8 and a fiber film 7, and installing a pulsation groove 5 and a culture frame 6; carefully fixing the high-elasticity film 8 and the fiber film 7 on the culture tank 13 in an aseptic environment, paying attention to the fact that the high-elasticity film 8 and the fiber film 7 are tightly attached and flatly laid without stress, forcibly inserting the culture rack 6 into the pulsating groove 5, inserting the protrusions 15 into the bottom surface of the culture tank 13, and inserting the strip-shaped protrusions 16 into the bottom surface of the strip-shaped grooves 12, so that the culture rack 6 and the pulsating groove 5 are tightly combined. The fourth step: inoculating cells; an appropriate amount of cell culture solution is added to the culture tank 13 and cells of an appropriate density are seeded. The fifth step: setting device parameters; the peristaltic pump 1 is set with a suitable rotation frequency according to the experimental requirements. And a sixth step: culturing cells; the device is put into an incubator and the operation of the device is started, the peristaltic pump 1 starts to periodically supply water to a culture system 4 through a hose 2, a part of hoses 2 connected with two ends of a water through hole 11 are fixed at the height of the highest point of a water tank 10 in a balanced manner, so that water in the water tank 10 is always in a contact state with a high-elasticity film 8, water flows into the water tank 10 through the water through hole 11 at one end, the water tank 10 starts to store water as the hoses 2 at two ends of the water through hole 11 are balanced with the highest point of the water tank 10, the water in the water tank 10 flows to the hose 2 at the other end while the water is stored in the water tank 10, a part of the hose 2 at the other end is balanced with the water tank 10, so that the water in the water tank 10 continues to store water, when the water in the water tank 10 is in contact with the high-elasticity film 8 at, the filter bottle 3 is used for exhausting air in the hose 2 to ensure that the water pressure is stably changed. The peristaltic pump 1 pumps water from the filter bottle 3 to form a complete loop, the peristaltic pump 1 continuously works to enable the water pressure in the water tank 10 to be continuously changed, the changed water pressure drives the high-elasticity film 8 covering the upper part of the water tank 10 to deform, the high-elasticity film 8 drives the fiber film 7 tightly attached to the high-elasticity film 8 to vibrate up and down, and further force stimulation is transmitted to cells cultured on the fiber film 7, so that the cell culture simulating blood vessel pulsation to apply mechanical stimulation is realized. The seventh step: observing cells; periodically changing the cell liquid and observing the growth condition of the cells.
Example 2:
a cell culture device for applying mechanical stimulation by simulating pulsation of blood vessels, which has a structure shown in FIGS. 6 to 8, is different from that of example 1 in that: the side surface of the water tank 10 is not provided with a strip-shaped groove 12 which is in clearance fit with the side surface of the water tank 10, and the protrusion 15 is not provided with a strip-shaped protrusion 16 which is matched with the strip-shaped groove 12.
When in use, the first step is as follows: cleaning and disinfecting the device; the peristaltic pump 1 and the hose 2 are scrubbed by 95% alcohol, the high-elasticity film 8 and the fiber film 7 are soaked by 95% alcohol overnight, the filter bottle 3, the pulsation groove 5 and the culture rack 6 are sterilized at high temperature, and finally, all parts are irradiated by ultraviolet lamps. The second step is that: preparing a cell culture solution; and selecting a corresponding solution formula to prepare the cell culture solution aiming at the selected cells and the culture purpose in an aseptic environment. The third step: installing a high-elasticity film 8 and a fiber film 7, and installing a pulsation groove 5 and a culture frame 6; carefully fixing the high-elasticity film 8 and the fiber film 7 on the culture tank 13 in an aseptic environment, paying attention to the fact that the high-elasticity film 8 and the fiber film 7 are tightly attached, flatly laid and not stressed, forcibly inserting the culture rack 6 into the pulsation groove 5, and inserting the protrusions 15 into the bottom surface of the culture tank 13 to enable the culture rack 6 and the pulsation groove 5 to be tightly combined. The fourth step: inoculating cells; an appropriate amount of cell culture solution is added to the culture tank 13 and cells of an appropriate density are seeded. The fifth step: setting device parameters; the peristaltic pump 1 is set with a suitable rotation frequency according to the experimental requirements. And a sixth step: culturing cells; the device is put into an incubator and the operation of the device is started, the peristaltic pump 1 starts to periodically supply water to a culture system 4 through a hose 2, a part of hoses 2 connected with two ends of a water through hole 11 are fixed at the height of the highest point of a water tank 10 in a balanced manner, so that water in the water tank 10 is always in a contact state with a high-elasticity film 8, water flows into the water tank 10 through the water through hole 11 at one end, the water tank 10 starts to store water as the hoses 2 at two ends of the water through hole 11 are balanced with the highest point of the water tank 10, the water in the water tank 10 flows to the hose 2 at the other end while the water is stored in the water tank 10, a part of the hose 2 at the other end is balanced with the water tank 10, so that the water in the water tank 10 continues to store water, when the water in the water tank 10 is in contact with the high-elasticity film 8 at, the filter bottle 3 is used for exhausting air in the hose 2 to ensure that the water pressure is stably changed. The peristaltic pump 1 pumps water from the filter bottle 3 to form a complete loop, the peristaltic pump 1 continuously works to enable the water pressure in the water tank 10 to be continuously changed, the changed water pressure drives the high-elasticity film 8 covering the upper part of the water tank 10 to deform, the high-elasticity film 8 drives the fiber film 7 tightly attached to the high-elasticity film 8 to vibrate up and down, and further force stimulation is transmitted to cells cultured on the fiber film 7, so that the cell culture simulating blood vessel pulsation to apply mechanical stimulation is realized. The seventh step: observing cells; periodically changing the cell liquid and observing the growth condition of the cells.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the present invention, but rather as the following description is intended to cover all modifications, equivalents and improvements falling within the spirit and scope of the present invention.
Claims (7)
1. A cell culture device for applying mechanical stimulation by simulating blood vessel pulsation comprises a power system, and is characterized in that the power system is connected with a culture system through a hose to form a connecting loop; the culture system comprises a pulsation groove and a culture rack in interference fit with the pulsation groove; a culture tank is arranged on the pulsation tank, and a water tank matched with the culture tank is arranged on the bottom surface of the culture tank; the water tank is provided with a high-elasticity film matched with the water tank, and the high-elasticity film is provided with a fiber film tightly matched with the high-elasticity film; the high-elasticity film and the fiber film are matched with the culture shelf.
2. The cell culture apparatus for applying mechanical stimulation by simulating pulsation of blood vessels according to claim 1, wherein the water tank has strip-shaped grooves on opposite sides thereof which are in clearance fit with the side surfaces of the water tank, and water passage holes on the other two sides thereof, and the outer side surface of any one of the strip-shaped grooves is flush with the inner surface of the culture tank, and the water passage holes are connected to the flexible tube through the pulsation groove.
3. The cell culture apparatus for applying mechanical stimulation by simulating vascular pulsation according to claim 2, wherein the water passage holes are all provided at the center of the side surface of the water tank, and the diameters of the water passage holes are all 1/2-5/6 of the depth of the water tank.
4. The cell culture device for applying mechanical stimulation by simulating blood vessel pulsation according to claim 1, wherein the culture frame is provided with openings symmetrical along the axis of the culture frame, the bottom surface of the culture frame is provided with protrusions which are in interference fit with the culture tank, the protrusions are arranged along the perimeter of the openings, and the protrusions are provided with a pair of strip-shaped protrusions which are matched with the strip-shaped grooves; and a culture rack cover matched with the opening is arranged on the top surface of the culture rack.
5. The cell culture device for applying mechanical stimulation by simulating pulsation of blood vessels according to claim 4, wherein the side surfaces of the culture racks protrude from the side surfaces of the pulsation groove.
6. The cell culture apparatus for applying mechanical stimulation by simulating vascular pulsation according to claim 1, wherein the power system comprises a peristaltic pump, the power system further comprises a filter flask connected with the peristaltic pump through a hose, and the filter flask is connected with the water through hole through a hose.
7. The cell culture apparatus for applying mechanical stimulation by simulating vascular pulsation according to claim 6, wherein the diameter of the water passage hole is equal to the diameter of the flexible tube; the culture shelf cover is a transparent cover plate; the hose is a silica gel hose.
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| CN201921332409.8U CN210340942U (en) | 2019-08-16 | 2019-08-16 | Cell culture device for applying mechanical stimulation by simulating blood vessel pulsation |
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| CN201921332409.8U CN210340942U (en) | 2019-08-16 | 2019-08-16 | Cell culture device for applying mechanical stimulation by simulating blood vessel pulsation |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113604358A (en) * | 2021-07-29 | 2021-11-05 | 中国科学院长春光学精密机械与物理研究所 | Cell loading device capable of replacing plane force and pneumatic control method |
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2019
- 2019-08-16 CN CN201921332409.8U patent/CN210340942U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113604358A (en) * | 2021-07-29 | 2021-11-05 | 中国科学院长春光学精密机械与物理研究所 | Cell loading device capable of replacing plane force and pneumatic control method |
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