CN1896223A - Disposable and closed aseptic thin-film filtering culturing dish - Google Patents
Disposable and closed aseptic thin-film filtering culturing dish Download PDFInfo
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- CN1896223A CN1896223A CN 200610019511 CN200610019511A CN1896223A CN 1896223 A CN1896223 A CN 1896223A CN 200610019511 CN200610019511 CN 200610019511 CN 200610019511 A CN200610019511 A CN 200610019511A CN 1896223 A CN1896223 A CN 1896223A
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Abstract
A disposable enclosed filtering culturing device of aseptic film consists of cylinder (1) and base (2), the cylinder (1) is inserted into base (2), top enclosed and base opened; liquid inlet (1.1) and vent hole are arranged on top of cylinder (1), rubber cap (1.3) is arranged at top of vent hole, air filtering membrane (1.4) is arranged at bottom, the base (2) is partitioned into upper and lower cylinders, the internal-wall of cylinder is lined with soft glue layer (2.1), liquid hole (2.2)is set in center, micro-film (2.3) is sealed arranged on top, and cellulose water-absorbing lining (2.4) is arranged at bottom. It is convenient and adaptable, includes single-stage, two-stage and multi-stage filter, and has filtering, culturing and bactericidal functions.
Description
Technical field
The present invention relates to a kind of microorganism detection thin-film filtering culturing dish; Specifically, relate to a kind of disposable use closed aseptic thin-film filtering culturing dish.
Background technology
Thin-film filtering culturing dish has a variety ofly in the market, but its function is generally comparatively single, can only carry out filter operation.Directly cultivate although there is minority to add liquid nutrient medium therein, do not see that can carry out filter operation can especially have the disposable use aseptic experiment articles for use of these characteristics by directly carrying out the membrane filter of enumeration after cultivating again.In addition, mostly the membrane filter diameter is about 50mm in the market, and is not suitable for film applicator coating bacterium, mold count.
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Summary of the invention
Purpose of the present invention just is to overcome the problems referred to above and the deficiency that prior art exists, and a kind of disposable use closed aseptic thin-film filtering culturing dish is provided.
The object of the present invention is achieved like this:
As Fig. 1, the present invention includes stack shell 1 and base 2, stack shell 1 inserts in the base 2;
Described stack shell 1 is the cylinder of top seal, bottom opening; Inlet opening 1.1 and ventilating pit 1.2 are arranged at stack shell 1 top; Rubber cap 1.3 is arranged at ventilating pit 1.2 tops, and air filtering membrane 1.4 is arranged at ventilating pit 1.2 bottoms;
Described base 2 is divided into two portions cylinder up and down by middle interval, and top cylinder is bigger, and the bottom cylinder is less; Cylinder inner wall is lined with flexible glue layer 2.1; There is fluid hole 2.2 in middle central authorities at interval; Middle top seal at interval is placed with microporous membrane 2.3; Microporous membrane has the Mierocrystalline cellulose liner 2.4 that draws water for 2.3 times; Middle lower central at interval has the fluid tubule 2.5 that links to each other with fluid hole 2.2.
Principle of work of the present invention is:
Above-mentioned thin-film filtering culturing dish is the single-stage thin-film filtering culturing dish, for the filtering clarifying liquid body and function.
The single-stage thin-film filtering culturing dish is connected by flexible pipe 3 with positive pressure pump (as peristaltic pump), starts positive pressure pump, liquid is moved in the single-stage thin-film filtering culturing dish filter; Flushing.When film applicator coating carries out microorganism detection or counting, liquid nutrient medium is directly added thin-film filtering culturing dish by positive pressure pump etc. and filter, the partially liq substratum draws the Mierocrystalline cellulose that invests under the microporous membrane 2.3 and draws water on the liner 2.4, extract and discard stack shell 1 then, standby loam cake is covered base 2, just base 2 is adapted as culture dish, puts in the incubator and cultivate, be applicable to microorganism detection or enumeration.
The present invention has the following advantages and positively effect:
1. Series Design comprises single-stage filtration, two-stage filtration and multi-joint filtration, and adaptability is strong, can be fit to use under the various situations.
2. Du Te Mierocrystalline cellulose liner 2.4 design that draws water, can easily liquid nutrient medium be drawn the Mierocrystalline cellulose that invests under the microporous membrane 2.3 draws water on the liner 2.4, cultivate by the substratum that Mierocrystalline cellulose draws water on the liner 2.4, and needn't prepare culture dish and solid medium in addition.
3. the present invention is formed by stack shell 1 insertion base 2, and is simple in structure, easy to use.The flexible glue layer design of base 2 inwall uniquenesses both guaranteed sealing property, made things convenient for again after filtering and washing and fast stack shell 1 extracted and discarded, and repacked base 2 into culture dish, integrated two big functions of strainer and culture dish.
4. stack shell (1) is transparent and the volume scale marks arranged, and observes and quantitatively convenient.
5. pass through sterilising packaging, disposable use.
Description of drawings
Fig. 1-structure iron of the present invention;
Wherein:
The 1-stack shell, the 1.1-inlet opening, the 1.2-ventilating pit,
1.3-rubber cap, 1.4-air filtration film,
1.5-primary filter film;
The 2-base, 2.1-flexible glue layer, the 2.2-fluid hole,
The liner 2.3-microporous membrane, 2.4-Mierocrystalline cellulose draw water,
2.5-fluid tubule.
The 3-flexible pipe.
Embodiment
According to the difference of filter liquide proterties, can be divided into single-stage thin-film filtering culturing dish, two-stage thin-film filtering culturing dish and multi-joint thin-film filtering culturing dish.
1, single-stage thin-film filtering culturing dish
As previously mentioned, the single-stage thin-film filtering culturing dish comprises stack shell 1 and base 2, and promptly stack shell 1 inserts in the base 2, uses for filtering clarifying liquid;
The design of base 2 inwalls has the flexible glue layer, seals;
Through sterilising packaging, disposable use.
2, two-stage thin-film filtering culturing dish
The two-stage thin-film filtering culturing dish is to have increased primary filter film 1.5 on the basis of single-stage thin-film filtering culturing dish, is that the suspendible sample is used for filtering non-clarified liq; Promptly be placed with primary filter film 1.5 at stack shell 1 in addition near the place, bottom, primary filter film 1.5 is by being ultrasonically welded within on the stack shell 1, the netted pad of primary filter film arranged below the primary filter film 1.5.
3, multi-joint thin-film filtering culturing dish
Multi-joint thin-film filtering culturing dish is by threeway or four-way flexible pipe 2～3 single-stages or two-stage thin-film filtering culturing dish to be composed in parallel, and sample liquid uniform distribution is wherein also filtered.
4, primary filter film 1.5 and microporous membrane 2.3 diameters are 50～80mm; Primary filter film 1.5 apertures are 0.45 μ m～10 μ m, and microporous membrane 2.3 apertures are 0.2 μ m～1 μ m.
Claims (6)
1, disposable use closed aseptic thin-film filtering culturing dish is characterized in that:
Comprise stack shell (1) and base (2), stack shell (1) inserts in the base (2);
Described stack shell (1) is the cylinder of top seal, bottom opening; Inlet opening (1.1) and ventilating pit (1.2) are arranged at stack shell (1) top; Rubber cap (1.3) is arranged at ventilating pit (1.2) top, and air filtering membrane (1.4) is arranged at the bottom;
Described base (2) is divided into two portions cylinder up and down by middle interval, and top cylinder is bigger, and the bottom cylinder is less; Cylinder inner wall is lined with flexible glue layer (2.1); There is fluid hole (2.2) in middle central authorities at interval; Middle top seal at interval is placed with microporous membrane (2.3); The Mierocrystalline cellulose liner (2.4) that draws water is arranged under the microporous membrane (2.3); Middle lower central at interval has the fluid tubule (2.5) that links to each other with fluid hole (2.2).
2, by the described thin-film filtering culturing dish of claim 1, it is characterized in that:
Be placed with primary filter film (1.5) at stack shell (1) near bottom place, the netted pad of primary filter film arranged below the primary filter film (1.5); Above-mentioned stack shell (1) inserts in the base (2) and promptly constitutes the two-stage thin-film filtering culturing dish.
3, by the described thin-film filtering culturing dish of claim 1, it is characterized in that:
By threeway or four-way flexible pipe 2～3 single-stages or two-stage thin-film filtering culturing dish are composed in parallel multi-joint thin-film filtering culturing dish.
4, by the described thin-film filtering culturing dish of claim 1, it is characterized in that:
Stack shell (1) and base (2) are all used the ebonite injection moulding, disposable shaping; Primary filter film (1.5) is by being ultrasonically welded within on the stack shell (1), and microporous membrane (2.3) is by being ultrasonically welded within on the base (2).
5, by the described thin-film filtering culturing dish of claim 1, it is characterized in that:
Stack shell (1) is transparent and the volume scale marks arranged.
6, by the described thin-film filtering culturing dish of claim 1, it is characterized in that:
Primary filter film (1.5) and microporous membrane (2.3) diameter are 50～80mm; Primary filter film (1.5) aperture is 0.45 μ m～10 μ m, and microporous membrane (2.3) aperture is 0.2 μ m～1 μ m.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610019511 CN1896223A (en) | 2006-06-30 | 2006-06-30 | Disposable and closed aseptic thin-film filtering culturing dish |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610019511 CN1896223A (en) | 2006-06-30 | 2006-06-30 | Disposable and closed aseptic thin-film filtering culturing dish |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1896223A true CN1896223A (en) | 2007-01-17 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200610019511 Pending CN1896223A (en) | 2006-06-30 | 2006-06-30 | Disposable and closed aseptic thin-film filtering culturing dish |
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| Country | Link |
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| CN (1) | CN1896223A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102634447A (en) * | 2012-04-18 | 2012-08-15 | 浙江万里学院 | Micro-array dialysis chamber and enrichment culture method using same |
| CN103922485A (en) * | 2014-04-04 | 2014-07-16 | 于美萍 | Sewage deodorizing process method |
| CN104548937A (en) * | 2014-10-29 | 2015-04-29 | 杭州泰林生物技术设备有限公司 | Film filtering device and method |
| CN105296350A (en) * | 2015-11-30 | 2016-02-03 | 苏州钧隆塑胶有限公司 | Bacterium cultivation bottle with multi-layer movable bottle bottoms |
| CN107849509A (en) * | 2015-07-24 | 2018-03-27 | 3M创新有限公司 | Film culture apparatus for enumeration of micro organisms |
| CN109266529A (en) * | 2018-10-11 | 2019-01-25 | 武汉友芝友医疗科技股份有限公司 | A kind of cell filter structure |
-
2006
- 2006-06-30 CN CN 200610019511 patent/CN1896223A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102634447A (en) * | 2012-04-18 | 2012-08-15 | 浙江万里学院 | Micro-array dialysis chamber and enrichment culture method using same |
| CN102634447B (en) * | 2012-04-18 | 2013-09-18 | 浙江万里学院 | Micro-array dialysis chamber and enrichment culture method using same |
| CN103922485A (en) * | 2014-04-04 | 2014-07-16 | 于美萍 | Sewage deodorizing process method |
| CN103922485B (en) * | 2014-04-04 | 2016-03-30 | 于美萍 | A kind of sewage deodorizing processing method |
| CN104548937A (en) * | 2014-10-29 | 2015-04-29 | 杭州泰林生物技术设备有限公司 | Film filtering device and method |
| CN107849509A (en) * | 2015-07-24 | 2018-03-27 | 3M创新有限公司 | Film culture apparatus for enumeration of micro organisms |
| CN107849509B (en) * | 2015-07-24 | 2022-04-08 | 3M创新有限公司 | Thin film culture device for counting microorganisms |
| US12077804B2 (en) | 2015-07-24 | 2024-09-03 | 3M Innovative Properties Company | Thin-film culture device for enumerating microorganisms |
| CN105296350A (en) * | 2015-11-30 | 2016-02-03 | 苏州钧隆塑胶有限公司 | Bacterium cultivation bottle with multi-layer movable bottle bottoms |
| CN109266529A (en) * | 2018-10-11 | 2019-01-25 | 武汉友芝友医疗科技股份有限公司 | A kind of cell filter structure |
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