CN2832824Y - Disposable filtering culture device using sterilized film - Google Patents
Disposable filtering culture device using sterilized film Download PDFInfo
- Publication number
- CN2832824Y CN2832824Y CN 200520096518 CN200520096518U CN2832824Y CN 2832824 Y CN2832824 Y CN 2832824Y CN 200520096518 CN200520096518 CN 200520096518 CN 200520096518 U CN200520096518 U CN 200520096518U CN 2832824 Y CN2832824 Y CN 2832824Y
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- stack shell
- sealing
- film
- disposable use
- use aseptic
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Abstract
The utility model discloses a disposable filtering culture device using a sterilized film, which relates to a film filtering culture device. The utility model comprises a sealing barrel body 1, a secondary barrel body 3, a base 4, a first microporous filtering film 6 and a microporous filtering film 7, and especially, the outer walls on the bottoms of the sealing barrel body 1 and the secondary barrel body 3 are respectively provided with a tear strip 1.6 of the sealing barrel body and a tear strip 3.3 of the secondary barrel body. A serial product of the utility model has the advantages of strong applicability and high filtering speed, and is matched with the diameter of a culture dish, which causes the operation efficiency of a culture medium to be enhanced. By using the tear strips and a top cover, the film fetching function and the culture function are caused to be combined into integration. The utility model has the advantages of convenient observation and quantitation.
Description
Technical field
The utility model relates to a kind of thin-film filtering culturing dish; Relate in particular to disposable use aseptic thin-film filtering culturing dish series, comprise single-stage enclosed filter incubator, two-stage enclosed filter incubator.
Background technology
Membrane filter (Membrane Filter) claim millipore filter again, is a kind of a kind of filtration unit by pollutent in millipore filtration removal liquid or the gas, can filter bacterium and molecule.The aperture of millipore filtration generally at 0.1 μ m between the 10 μ m.Membrane filter has been widely used in holding back particulate, bacterium and other impurity from gas phase or liquid phase, to reach the purpose of separation, purification, purification, analysis.
In the market and the bigger membrane filter of the diameter of bibliographical information, mostly its diameter is that about 50mm, filtration velocity is slower, and the culture dish diameter is generally about 85mm, when film applicator coating carries out the microorganism culturing analysis, do not match each other, the service efficiency of substratum is low; Its two, for solid sample or not clarifying liquid and since sample liquid in have insoluble particle, directly influence the filtration velocity of smaller aperture due (as 0.45 μ m) millipore filtration; Its three, general closed membrane filter is fixed on microporous membrane in the strainer by ultrasonic wave or viscose glue, can not take out; Its four, general open membrane filter can not directly add liquid nutrient medium in the strainer and cultivate after filtration is finished; Its five, existing assembly type membrane filter, great majority after filtration is finished, need be turned on screw thread for being threaded usually on the structure, could be from membrane filter base the inside taking-up millipore filtration, inconvenient operation; Its six, the membrane filter on the market mostly is repeatedly and uses, and needs to clean, assembling and sterilization, working strength is big; Through repeatedly using, seal bad.
Summary of the invention
The purpose of this utility model just is to overcome the problem and shortage that prior art exists, and a kind of disposable use aseptic thin-film filtering culturing dish is provided.
The purpose of this utility model is to realize like this.
As Fig. 1, comprise sealing stack shell 1 and base 4, connect up and down by bayonet socket or ultrasonic wave or viscose glue, the centre accompanies second millipore filtration 7.
Described sealing stack shell 1 is a kind of no round-ended cylinder; The cylinder top is provided with inlet opening 1.1 and sealing stack shell ventilating pit 1.2; Sealing stack shell ventilating pit 1.2 is built-in with sealing stack shell air filtration film 1.4 and sealing stack shell ventilating pit plug or Capsule 1.3;
Described base 4 is a kind of cylinder; At the cylinder top, be provided with circular in it or strip groove 4.2, be provided with fluid hole 4.3 and fluid hole plug or Capsule 4.4 in the cylinder bottom.
Described second millipore filtration 7 is whole filter membrane, is a kind of membrane filter media.
Principle of work of the present utility model is:
Single-stage enclosed filter incubator is used for filtering more clarifying liquid.
As Fig. 1, single-stage enclosed filter incubator is connected with positive pressure pump (as peristaltic pump) or vacuum pump, open fluid hole plug or Capsule 4.4, liquid is moved in the single-stage enclosed filter incubator filter; And it is flushable.Carry out the microorganism culturing analysis at film applicator coating, or other purposes is need take out second millipore filtration 7 time, after filtering and washing, close positive pressure pump (as peristaltic pump) or vacuum pump, be torn by hand sealing stack shell tear strip 1.6, unload sealing stack shell 1, can expose second millipore filtration 7, take out second millipore filtration 7 with aseptic nipper, facing up is affixed on the plate nutrient agar, put in the incubator and cultivate, or be used for other purposes; At needs liquid nutrient medium is directly added when cultivating in the filtering culturing dish, after filtering and washing, close fluid hole plug or Capsule 4.4, open sealing stack shell ventilating pit plug or Capsule 1.3, utilize positive pressure pump (as peristaltic pump) that liquid nutrient medium is directly added in the filtering culturing dish, put in the incubator and cultivate.
The utlity model has following advantage and positively effect:
1. be a kind of series product, adaptability is strong, can be fit to use under the various situations.
2. the filtration velocity height again with culture dish diameter coupling, improves the service efficiency of substratum.
3. utilize tear strip and top cover, make and get film after the filtration and cultivate two big functions and be unified in one.
4. can implement the successive two-stage filtration, can filter clarifying liquid fast, can filter not clarifying liquid fast again.
5. stack shell is transparent and the volume scale marks arranged, and observes and quantitatively convenient.
6. pass through sterilising packaging, disposable use.
Description of drawings
Fig. 1-single-stage enclosed filter incubator synoptic diagram;
Fig. 2-two-stage enclosed filter incubator synoptic diagram.
Wherein:
1-seals stack shell,
1.1-inlet opening, 1.2-sealing stack shell ventilating pit,
1.3-sealing stack shell ventilating pit plug or Capsule, 1.4-sealing stack shell air filtration film,
1.5-convex clip in the sealing stack shell, 1.6-sealing stack shell tear strip;
3-level stack shell,
3.1-inferior level stack shell indent card, the porose backing plate of 3.2-,
3.3-inferior level stack shell tear strip, convex clip in 3.4-the level stack shell,
3.5-inferior level stack shell ventilating pit, 3.6-level stack shell air filtration film,
3.7-inferior level stack shell ventilating pit plug or Capsule;
The 4-base,
4.1-base indent card, the 4.2-groove,
4.3-fluid hole, 4.4-fluid hole plug or Capsule;
6-first millipore filtration; 7-second millipore filtration;
Embodiment
Describe in detail below in conjunction with drawings and Examples:
As Fig. 1, be a kind of single-stage enclosed filter incubator;
As Fig. 2, be a kind of two-stage enclosed filter incubator, sealing stack shell 1, secondary stack shell 3 and base 4 are connected by bayonet socket or ultrasonic wave or viscose glue successively; Accompany first millipore filtration 6 between sealing stack shell 1 and the secondary stack shell 3, accompany second millipore filtration 7 between secondary stack shell 3 and the base 4.
Described secondary stack shell 3 is a kind of cylinder that does not have the end; At the cylinder top, porose backing plate 3.2 is set in it, the cylinder upper portion side wall is provided with secondary stack shell ventilating pit 3.5; Secondary stack shell ventilating pit 3.5 is built-in with secondary stack shell air filtration film 3.6 and secondary stack shell ventilating pit plug or Capsule 3.7;
Described first millipore filtration 6 is pre-filter membrane, is a kind of jumbo Depth Filtration medium.
Two-stage enclosed filter incubator is used for filtering not clarifying liquid.
As Fig. 2, two-stage enclosed filter incubator is connected with positive pressure pump (as peristaltic pump) or vacuum pump, open fluid hole plug or Capsule 4.4, liquid is moved in the two-stage enclosed filter incubator filter; And it is flushable.Carry out the microorganism culturing analysis at film applicator coating, or other purposes is need take out second millipore filtration 7 time, after filtering and washing, close positive pressure pump (as peristaltic pump) or vacuum pump, be torn by hand secondary stack shell tear strip 3.3, unloading secondary stack shell 3 reaches with top, can expose second millipore filtration 7, take out second millipore filtration 7 with aseptic nipper, facing up is affixed on the plate nutrient agar, put in the incubator and cultivate, or be used for other purposes; At needs liquid nutrient medium is directly added when cultivating in the filtering culturing dish, after filtering and washing, close fluid hole plug or Capsule 4.4, open secondary stack shell ventilating pit plug or Capsule 3.7, utilize positive pressure pump (as peristaltic pump) that liquid nutrient medium is directly added in the filtering culturing dish, put in the incubator and cultivate.
* seal stack shell 1 bottom sidewall and be provided with convex clip 1.5 in the sealing stack shell; Secondary stack shell 3 upper portion side wall are provided with secondary stack shell indent card 3.1, and the bottom sidewall is provided with convex clip 3.4 in the secondary stack shell; Base 4 upper portion side wall are provided with base indent card 4.1.
The diameter of sealing stack shell 1 * of the present utility model and secondary stack shell 3 is 40-80mm, can place first millipore filtration 6 and second millipore filtration 7 of the about 40-80mm of diameter, has improved filtration velocity significantly; When film applicator coating carries out the microorganism culturing analysis, the service efficiency height of substratum can be mated with the culture dish diameter.
* sealing stack shell 1 of the present utility model and secondary stack shell 3 are made by the injection moulding of transparent flexible plastic cement, and outer wall or inwall are provided with the volume scale marks, the convenient observation with quantitative.
* the utility model is respectively arranged with sealing stack shell tear strip 1.6 and secondary stack shell tear strip 3.3 on the bottom sidewall of sealing stack shell 1 and secondary stack shell 3, can conveniently take out second millipore filtration 7.
* described sealing stack shell tear strip 1.6 and secondary stack shell tear strip 3.3, its structure are a kind of slice circular ring plate of soft thin-walled, and all ultra-thin-wall is connected on sealing stack shell 1 and the secondary stack shell 3 up and down, and handle is arranged, and conveniently is torn by hand.
* the aperture of first millipore filtration 6 is 0.8 μ m-10 μ m, can filter heavy-gravity, not clarifying difficult filtered liquid; The particulate that second millipore filtration 7 must be held back significantly reduces; Thereby increased the working efficiency of filter membrane, guaranteed high flow rate.
* the aperture of second millipore filtration 7 is 0.1 μ m-1 μ m; White or colored (black, green etc.) are printed on gridline; Peripheral circles is handled through hydrophober, makes the edge form a circle hydrophobic band.When film applicator coating carried out the microorganism culturing analysis, counting was convenient, also was convenient to flushing.
* the utility model is through sterilising packaging, disposable use.
* the utility model is furnished with the aseptic special-purpose tweezers of disposable use, convenient second millipore filtration 7 that takes out.
Claims (10)
1, disposable use aseptic thin-film filtering culturing dish is characterized in that:
Comprise sealing stack shell (1) and base (4), connect up and down that the centre accompanies second millipore filtration (7);
Described sealing stack shell (1) is a kind of no round-ended cylinder; The cylinder top is provided with inlet opening (1.1) and sealing stack shell ventilating pit (1.2); Sealing stack shell ventilating pit (1.2) is built-in with sealing stack shell air filtration film (1.4) and sealing stack shell ventilating pit plug or Capsule (1.3);
Described base (4) is a kind of cylinder; At the cylinder top, be provided with circular in it or strip groove (4.2); Be provided with fluid hole (4.3) and fluid hole plug or Capsule (4.4) in the cylinder bottom;
Described second millipore filtration (7) is whole filter membrane, is a kind of membrane filter media.
2, by the described disposable use aseptic thin-film filtering culturing dish of claim 1, it is characterized in that:
Sealing stack shell (1), secondary stack shell (3) are connected successively with base (4); Accompany first millipore filtration (6) between sealing stack shell (1) and the secondary stack shell (3), accompany second millipore filtration (7) between secondary stack shell (3) and the base (4);
Described secondary stack shell (3) is a kind of cylinder that does not have the end; At the cylinder top, porose backing plate (3.2) is set in it, the cylinder upper portion side wall is provided with secondary stack shell ventilating pit (3.5); Secondary stack shell ventilating pit (3.5) is built-in with secondary stack shell air filtration film (3.6) and secondary stack shell ventilating pit plug or Capsule (3.7);
Described first millipore filtration (6) is pre-filter membrane, is a kind of jumbo Depth Filtration medium.
3, by the described disposable use aseptic thin-film filtering culturing dish of claim 1, it is characterized in that:
Sealing stack shell (1) bottom sidewall is provided with convex clip (1.5) in the sealing stack shell; Secondary stack shell (3) upper portion side wall is provided with secondary stack shell indent card (3.1), and the bottom sidewall is provided with convex clip (3.4) in the secondary stack shell; Base (4) upper portion side wall is provided with base indent card (4.1).
4, by the described disposable use aseptic thin-film filtering culturing dish of claim 1, it is characterized in that:
The diameter of sealing stack shell (1) and secondary stack shell (3) is 40-80mm.
5, by the described disposable use aseptic thin-film filtering culturing dish of claim 1, it is characterized in that:
The material of sealing stack shell (1) and secondary stack shell (3) is the transparent flexible plastic cement, and outer wall or inwall are provided with the volume scale marks.
6, by the described disposable use aseptic thin-film filtering culturing dish of claim 1, it is characterized in that:
Be respectively arranged with sealing stack shell tear strip (1.6) and secondary stack shell tear strip (3.3) on the bottom sidewall of sealing stack shell (1) and secondary stack shell (3).
7, by the described disposable use aseptic thin-film filtering culturing dish of claim 1, it is characterized in that:
The structure of sealing stack shell tear strip (1.6) and secondary stack shell tear strip (3.3) is a kind of slice circular ring plate of soft thin-walled, and handle is arranged.
8, by the described disposable use aseptic thin-film filtering culturing dish of claim 1, it is characterized in that:
The aperture of first millipore filtration (6) is 0.8 μ m-10 μ m.
9, by the described disposable use aseptic thin-film filtering culturing dish of claim 1, it is characterized in that:
The aperture of second millipore filtration (7) is 0.1 μ m-1 μ m.
10, by the described disposable use aseptic thin-film filtering culturing dish of claim 1, it is characterized in that:
Second millipore filtration (7) is a white or colored, is provided with gridline, and peripheral circles is a circle hydrophobic band.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200520096518 CN2832824Y (en) | 2005-05-26 | 2005-05-26 | Disposable filtering culture device using sterilized film |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200520096518 CN2832824Y (en) | 2005-05-26 | 2005-05-26 | Disposable filtering culture device using sterilized film |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200620114591 Division CN2898048Y (en) | 2005-05-26 | 2005-05-26 | Filtering culture dish of disposable opening aseptic thin film |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2832824Y true CN2832824Y (en) | 2006-11-01 |
Family
ID=37197137
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200520096518 Expired - Fee Related CN2832824Y (en) | 2005-05-26 | 2005-05-26 | Disposable filtering culture device using sterilized film |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2832824Y (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101665768B (en) * | 2008-09-05 | 2012-07-25 | 中国人民解放军第三军医大学第二附属医院 | Bioreactor for in vitro culture of neurotrophic factors |
| CN101294135B (en) * | 2007-04-26 | 2013-02-06 | Emd密理博公司 | Microbiological analysis assembly and method |
-
2005
- 2005-05-26 CN CN 200520096518 patent/CN2832824Y/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101294135B (en) * | 2007-04-26 | 2013-02-06 | Emd密理博公司 | Microbiological analysis assembly and method |
| CN101665768B (en) * | 2008-09-05 | 2012-07-25 | 中国人民解放军第三军医大学第二附属医院 | Bioreactor for in vitro culture of neurotrophic factors |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |