JP2001309776A - Device for collecting microorganism - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide at a low price a new organism-collecting device which has excellent work efficiency and can inhibit the clogging of a filter. SOLUTION: This organism-collecting device characterized by comprising an integrally molded resin housing having a filter-receiving portion 24, a funnel- loading portion 25 disposed on the outside of the filter-receiving portion 24 at a slightly higher level, a surrounding wall 26 for surrounding the funnel- loading portion 25, and a drain port 28 opened in the filter-receiving portion 24, a membrane filter 30 received in the filter-receiving portion 24 of the housing to collect target microorganisms in a specimen, a paper funnel 33 having a bottom surface 35 which acts as a prefilter for collecting food residues and microorganisms having coarser particle sizes than that of the target microorganisms in the specimen, and a resin seal ring 22 which is loaded on the funnel-loading portion 25 of the housing in a state fit to the bottom face- surrounding portion 36 of the paper funnel and fit to the surrounding wall to attach the paper funnel in a liquid-tight state.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a microorganism collecting apparatus, and more particularly to a microorganism collecting apparatus suitably used for capturing microorganisms on a membrane filter.

[0002]

2. Description of the Related Art In recent years, biologically inactive materials (for example, PES = polyethersulfone, PV
The HGMF method (hydrophobic lattice membrane filter method) in which a specimen is passed through a membrane filter formed of DF (polyvinylidene difluoride or the like) and the microorganisms in the specimen are collected on the membrane filter has been widely used. I have.

[0003] As an apparatus for performing a microorganism test by the HGMF method, for example, an apparatus in which a glass funnel and a storage bottle are connected by a connector on which a membrane filter is set is provided. According to this device, when a predetermined amount of the sample contained in the funnel passes through the membrane filter, microorganisms in the sample are collected. In addition, multiple funnels with a membrane filter set on the bottom are attached to the manifold,
In addition, there is also provided an apparatus configured so that microorganisms can be efficiently collected on a plurality of membrane filters at a time by suction under reduced pressure from the manifold.

In these conventional devices, PP and PE are used.
A filter unit is used in which a membrane filter is mounted on the bottom surface of a dish-shaped housing integrally formed of a synthetic resin such as the above. After passing the specimen, the filter unit is removed from the apparatus, and only the membrane filter is removed from the filter unit, and the microorganism is cultured on a predetermined medium in a petri dish to perform a microorganism test.

[0005]

However, when the above-mentioned conventional apparatus is used, the membrane filter is immediately clogged if the microorganism particles in the sample are large and coarse.

There has been proposed a disposable type in which a membrane filter is previously set at the bottom of the funnel and integrated, but the conventional type is entirely made of resin in order to fit the funnel and the membrane filter in a liquid-tight manner. Yes, it could not be provided at a low price, so it has not yet spread.

[0007]

SUMMARY OF THE INVENTION Accordingly, the present invention solves the above-mentioned problems in the prior art, and provides a novel microorganism collecting apparatus which is excellent in work efficiency and can suppress filter clogging at a low cost. The purpose is to:

In order to achieve this object, the present invention uses an inexpensive paper funnel. Furthermore, by using the paper funnel whose bottom surface is a filter paper, it functions as a pre-filter, and collects a substance having a coarse particle diameter in the sample, thereby clogging the membrane filter. To prevent

A major problem in the case of using a paper funnel is that it is difficult to make the fitting state when integrated with a membrane filter liquid-tight. By attaching the peripheral edge of the bottom surface of the paper funnel to the housing in a liquid-tight manner, it is possible to secure the liquid-tight fitting state and completely prevent the leakage of the specimen.

[0010] That is, the microorganism collecting apparatus according to the present invention according to the present invention comprises a filter accommodating portion, a funnel mounting portion provided at a level slightly higher than the filter accommodating portion outside the filter accommodating portion, and the filter accommodating portion. A housing integrally formed of resin having an outlet opening into the housing, and a membrane filter housed in a filter housing of the housing for collecting target microorganisms in the sample,
A paper cup-shaped funnel whose bottom edge is liquid-tightly mounted on the funnel mounting portion of the housing via a resin seal means, and whose bottom surface has a coarser particle size than the target microorganism in the sample. And a paper funnel functioning as a pre-filter for collecting the residue.

According to a second aspect of the present invention, in the microorganism collecting apparatus according to the first aspect, a porous material is accommodated in a lower accommodating portion recessed in the filter accommodating portion, and the membrane filter is mounted on the porous material. It is characterized by being supported.

According to a third aspect of the present invention, there is provided the microorganism collecting apparatus according to the second aspect, wherein projections are provided at intervals on a bottom surface of the lower housing portion of the housing, and the porous material is placed on the projections and is provided with the projections. It is characterized by being housed in the lower housing.

According to a fourth aspect of the present invention, in the microorganism collecting apparatus according to the second or third aspect, the porous material accommodated in the lower accommodation portion of the housing is PP (polypropylene) or filter paper.

In one preferred embodiment of the present invention, the outer peripheral portion of the housing has a double wall structure, and the gap between the double walls is filled with a soft silicone resin, and the space between the double walls is made of paper. By adopting a means of inserting and integrating the periphery of the bottom surface of the funnel, it is possible to secure the liquid-tight fitting state of the paper funnel and completely prevent the leak of the sample. According to this embodiment, it is configured as an all-in-one disposable type unit in which all the components are integrated, and is provided to the market in a state sterilized by γ-ray irradiation or the like.

[0015] That is, a microorganism collecting apparatus according to the present invention according to claim 5 includes a filter accommodating portion, a double wall structure portion provided outside the filter accommodating portion at a level slightly higher than the filter accommodating portion, and A lower housing recessed in the filter housing, a housing integrally formed with a discharge port opened to the lower housing, and a filter of the housing for collecting target microorganisms in a sample; Paper whose peripheral edge is inserted into the space between the membrane filter housed in the housing and the double-walled structure of the housing, and which is fixed in a liquid-tight manner by the resin sealant filled in the space. A glass funnel, the bottom surface of which functions as a pre-filter for collecting microorganisms and food residues whose particle size is coarser than the target microorganism in the sample; It is a porous material which is accommodated in the lower housing portion of the housing to support the emission filter from below, characterized in that it comprises a.

According to a sixth aspect of the present invention, there is provided the microorganism collecting apparatus according to the fifth aspect, wherein a substantially ring-shaped packing for further pressing the membrane filter from above is detachably provided in the filter accommodating portion of the housing. .

According to a seventh aspect of the present invention, in the microorganism collecting apparatus according to the sixth aspect, the membrane filter is accommodated in a filter accommodating portion of the housing while being fixed to a bottom of the packing.

According to an eighth aspect of the present invention, in the microorganism collecting apparatus according to any one of the fifth to seventh aspects, the resin sealant filled in the space between the double wall structures of the housing is silicon. Features.

In another preferred embodiment of the present invention, a resin seal ring integrally formed in a predetermined shape is used in advance, and the bottom surface of the paper funnel is fitted to the resin seal ring and fixed to the housing in a liquid-tight manner. By adopting such a means, it is possible to secure the liquid-tight fitting state of the paper funnel and to completely prevent the leak of the specimen. According to this embodiment, since the resin seal ring can be detached from both the paper funnel and the housing, the resin seal ring and the housing are relatively expensive and do not directly touch the sample. Can be reused by subjecting it to an autoclave sterilization process, and is provided as a microorganism collection device in which other components are disposable.

In other words, the microorganism collecting apparatus according to the present invention has a filter accommodating portion, a funnel mounting portion provided at a level slightly higher than the filter accommodating portion outside the filter accommodating portion, and the funnel accommodating portion. A housing integrally formed of resin having a peripheral wall surrounding the mounting portion and an outlet opening to the filter housing portion, and a housing housed in the filter housing portion of the housing for collecting target microorganisms in a sample. A membrane filter, a paper funnel having a bottom surface functioning as a pre-filter for collecting microorganisms and food residues coarser in particle size than the target microorganism in the sample, and a state in which the bottom edge of the paper funnel is fitted. A resin seal ring mounted on the funnel mounting portion of the housing and fitted into the peripheral wall to mount the paper funnel in a liquid-tight manner. It is characterized in that Ete made.

According to a tenth aspect of the present invention, in the microorganism collecting apparatus according to the ninth aspect, the resin seal ring is detachable from both the paper funnel and the housing.

In a preferred embodiment, the bottom surface of the resin seal ring extends inward from the funnel mounting portion of the housing to above the filter housing portion. The inner extension portion presses the outer peripheral portion of the membrane filter housed in the filter housing portion.

According to a twelfth aspect of the present invention, there is provided the microorganism collecting apparatus according to any one of the ninth to eleventh aspects, wherein an outer peripheral edge of the resin seal ring has a double-walled structure, and a space between the double-walled structure is provided. The bottom edge of the paper funnel is fitted into the gap, and the outer wall surface of the double-walled structure is attached in close contact with the inner wall surface of the housing.

According to a thirteenth aspect, in the microorganism collecting apparatus according to any one of the ninth to twelfth aspects, the resin seal ring is made of silicon.

According to a fourteenth aspect of the present invention, there is provided the microorganism collecting apparatus according to any one of the ninth to thirteenth aspects, wherein a protrusion protruding inward is provided at an upper end of the peripheral wall of the housing, and the protrusion presses the upper end of the resin seal ring. It is characterized in that it does not lift up.

According to a fifteenth aspect, in the microorganism collecting apparatus according to any one of the ninth to fourteenth aspects, a part of the upper end of the peripheral wall of the housing projects outward to form a projecting piece.

According to a sixteenth aspect of the present invention, in the microorganism collecting apparatus according to any one of the ninth to fifteenth aspects, the membrane filter is housed in a filter housing portion of the housing in a state where the membrane filter is overlaid on filter paper. .

According to a seventeenth aspect, in the microorganism collecting apparatus according to the sixteenth aspect, a plurality of radial projections are provided at intervals in a concave portion provided in the filter accommodating portion, and the membrane filter is provided on the radial projections. It is characterized in that it is placed in a state of being superimposed on the filter paper.

[0029]

1 and 2 show an embodiment of the present invention, in which a paper funnel 12 is fixed to a housing 1 which is a resin-integrated product mainly made of PP, acrylic resin, HDPE or the like. This is a disposable type microorganism collection kit with an all-in-one structure.

The housing 1 houses a membrane filter 3 made of a biologically inactive material such as PES or PVDF in a filter housing portion 2 at the center thereof, and a peripheral portion of the membrane filter 3 is made of a substantially ring-shaped resin. (In this embodiment, HIPS =
It is pressed down by a packing 4 made of (polystyrene) to prevent lifting.

A slightly smaller diameter lower housing 5 is provided below the filter housing 2 of the housing 1, and the porous material 6 housed therein accommodates the membrane filter 3.
Is supported from below. The porous material 6 supports the membrane filter 3 and plays a role of applying a reduced-pressure suction force from an outlet 7 connected to a reduced-pressure suction source (not shown) such as a vacuum pump to the membrane filter 3. Although porous resins, porous glass and other porous materials can be used, PP can be used at a low cost.
Or filter paper is particularly suitable.

The porous material 6 is placed on ridges provided at intervals on the bottom surface of the lower housing 2 in order to perform the above-mentioned vacuum suction more smoothly. In the illustrated embodiment, narrow ribs 8 extending radially except for the outlet 7 are formed at regular intervals with a central angle of 60 degrees.

The outer peripheral portion of the housing 1 includes an inner wall 9a and an outer wall 9
and a double-walled structure portion 9 formed by the resin sealing agent 11 is filled with a soft or liquid resin sealing agent 11 such as silicon.

The paper funnel 12 is in the form of a paper cup with an open upper surface, and has a peripheral edge portion 15 whose peripheral side surface 13 extends further beyond the bottom surface 14 and projects downward.
Then, the peripheral portion 15 is inserted into the space 10 between the double wall structure portions 9 of the housing 1 and fixed by a resin sealant 11 such as silicon.

The bottom surface 14 of the paper funnel 12 is made of filter paper having a function as a pre-filter for thinning microorganisms having a particle size smaller than the target microorganism (bacteria) in the sample in advance. Filter paper.

In order to manufacture the microorganism collection kit configured as described above, the housing 1 is made of a resin such as PP or acrylic resin by connecting the filter housing 2, the lower housing 5, the double wall structure 9, and the discharge port 7. After being integrally formed into a predetermined shape having the same, a porous material 6 such as PP or filter paper is accommodated in a lower accommodating portion 5, and a membrane filter 3 and a packing 4 are accommodated in a filter accommodating portion 2 thereon. Wall structure 9
The resin sealant 11 such as silicon is injected into the gap 10 between the two to make the semi-cured state, and then the bottom edge 15 of the paper funnel 12 is inserted into the gap 10 to form the resin sealant 11.
Fix with.

It is preferable that the peripheral edge of the membrane filter 3 is fixed in advance to the bottom of the packing 4 with an adhesive or the like, and the membrane filter 3 is mounted on the porous material 6 using tweezers or the like.

The microbial collection kits thus produced are individually sealed and packaged with a resin film, and are then accommodated in a plurality of (for example, 300) cases such as a cardboard box and irradiated with γ-rays (for example, 15 KGY). To be sterilized and provided to the market.

The usage of such a microorganism collection kit will be described below. A solution to be tested for microorganisms is discharged at a predetermined flow rate with a vacuum suction source (not shown) such as a vacuum pump connected to the outlet 7. Into the funnel 12.

Microorganisms (Daphnia) and food residues having a particle size larger than the target microorganism (bacteria) contained in the solution are about 10%.
Since it cannot pass through the funnel bottom 14 which is a 0 μm mesh, it is trapped by this and does not cause clogging of the membrane filter 3.

The solution that has passed through the funnel bottom surface 14 then passes through the membrane filter 3, and at this time, bacteria having a size of 1 to 4 μm, which is the target microorganism, are passed through the membrane filter 3.
Is captured by The solution that has passed through the membrane filter 3 is stored in a predetermined tank (not shown) via the porous material 6 and the discharge port 7, or is discharged out of the system as it is.

A part of the solution introduced into the paper funnel 12 may flow into the space 10 beyond the inner wall 9a of the double-walled structure 9 of the housing 1. Since the cured resin sealant 11 keeps the fitting of the paper funnel 12 to the housing 1 in a liquid-tight manner, there is no leakage to the outside.

After passing a predetermined amount of the solution through the membrane filter 3 in this manner, the packing 4 is lifted with tweezers or the like, and the membrane filter 3 adhered to the packing 4 is removed from the housing 1, and a petri dish (not shown)
And cultured in a predetermined medium.

In the embodiment described above, the housing 1
Paper funnel 1 in the gap 10 between the double wall structures 9
2, the space 10 is filled with a resin sealant 11 such as silicone, and the paper funnel 12 is fixed by curing the resin sealant 11.
May be used, a resin seal ring 16 previously formed into a shape as shown in FIGS. 3 and 4 using a resin material such as silicon may be used.

The resin seal ring 16 is tightly fitted into the gap 10 between the double-walled structures 9 and has a shape. The resin seal ring 16 is fitted into the cavity 10 by inserting and fitting the outer wall 9b of the double wall structure portion 9 into the concave groove 17. ing. Protrusions 19 and 20 are formed on the inner and outer peripheries of the lower end, and are pressed into contact with the inner wall 9a and the outer wall 9b of the double-walled structure portion 9 so that the fitted state of the resin seal ring 16 is made liquid-tight. keeping.

The main body of the resin seal ring 16 has an insertion groove 21 opening upward, and the bottom peripheral edge 15 of the paper funnel 12 is tightly inserted into the insertion groove 21.

By adopting the above configuration, the resin seal ring 16 is fitted in the housing 1 in a liquid-tight manner in the assembled state and the paper funnel 1
2 fits in a liquid-tight manner, but is detachable from both. Therefore, the housing 1 and the resin seal ring 16 can be reused after being autoclaved, which contributes to a reduction in total cost.

FIG. 5 shows a reusable type microorganism collection kit according to another embodiment of the present invention using a resin seal ring 22 having a shape slightly different from that shown in FIGS. 3 and 4. A similar front half sectional view is shown.

The housing 23 is a resin-integrated product mainly made of PP, acrylic resin, HDPE, or the like. The housing 23 has a filter housing portion 24 and a funnel mounting portion 25 provided at a slightly higher level outside the filter housing portion 24.
And a peripheral wall 26 surrounding the funnel mounting portion 25, and a discharge port 28 opening to the filter housing portion 24.

PES or PVDF is stored in the filter housing 24.
The membrane filter 30 formed of a biologically inert material such as is accommodated in a state of being superposed on a filter paper 31 having a thickness of about 1 mm, and these peripheral portions are pressed from above by a resin seal ring 22. Floating is prevented.

A slightly smaller diameter concave portion 27 is formed concentrically in the filter accommodating portion 24. A plurality of ribs 32 are radially provided at intervals in the recess 27, and the filter paper 3
1 and the membrane filter 30 are supported from below.

The ribs 32 perform substantially the same function as the radial ribs 8 (FIG. 2) in the above-described embodiment, but in this embodiment, a thin filter paper 31 is used as a porous material.
And is directly mounted on the rib 32. Therefore, it is possible to prevent these from being sucked into the outlet 28 while sufficiently applying the reduced-pressure suction force from a reduced-pressure suction source (not shown) such as a vacuum pump to the filter paper 31 / membrane filter 30. For this reason, it is preferable to provide a plurality of radial ribs 29 over the diameter of the recess 27 so as to extend over the outlet 28 (see FIG. 8). In FIG. 8, the radial ribs 29 are provided at equal intervals at a central angle of 45 degrees, but it goes without saying that more ribs may be provided at smaller intervals. In addition, as shown in FIG. 8, in addition to the radial rib 29 extending over the outlet 28, a radial rib 45 can be provided outside the outlet 28. The upper surfaces of these radial ribs 29 and 45 are
In order to mount the membrane filter 30, it is formed flush with the filter accommodating portion 24. These ribs 29, 45
The number and the intervals are arbitrary, and FIG. 8 shows only one example.

A paper funnel 33 is fitted on the funnel mounting portion 25 and in the peripheral wall 26 in a liquid-tight manner. In this embodiment, the liquid-tight fitting of the funnel is easily performed by using the resin seal ring 22 which is detachably attached to both the housing 23 and the paper funnel 33. Are shown in FIGS. 6 and 7.

The paper funnel 33 used in this embodiment is the same as the paper funnel 12 in the above embodiment.
A peripheral edge portion 36 is formed in a paper cup shape substantially similar to that of FIG.
have. Also, the bottom surface 35 of the paper funnel 33
Is constituted by a filter paper having a function as a pre-filter for previously capturing microorganisms having a particle size coarser than a target microorganism (bacteria) in a specimen, and is, for example, a filter paper of about 100 μm mesh.

Referring to FIGS. 6 and 7, the resin seal ring 22 used in this embodiment is an integrally molded product made of silicone resin and has a bottom surface portion mounted on the funnel mounting portion 25 of the housing 23. 37 and bottom part 3
7 has an inner peripheral wall 38 and an outer peripheral wall 39 which stand substantially upright, and an upward insertion groove 40 formed between the inner and outer peripheral walls. When the bottom portion 37 is placed on the funnel placing portion 25, the outer peripheral wall 39 comes into close contact with the inner surface of the housing peripheral wall 26. The insertion groove 40 is formed on the bottom edge 36 of the paper funnel 33.
Is formed at a position having a diameter dimension corresponding to. That is, the resin seal ring 22 has the bottom surface portion 37 placed on the funnel placement portion 25 and the outer peripheral wall 39 with the bottom surface peripheral portion 36 of the paper funnel 33 inserted and fitted into the insertion groove 40. Can be closely fitted to the housing peripheral wall 26.

In order to manufacture the microorganism collection kit of this embodiment, the housing 23 is integrally formed of resin such as acrylic into the above-mentioned predetermined shape, and then the filter paper is placed on the projections 32 (ribs 29, 45) provided in the recess 27. 31 alone or the filter paper 31 is not placed.

On the other hand, the bottom edge 3 of the paper funnel 33
6 is inserted into the insertion groove 40 of the resin seal ring 22, and the paper funnel 33 is temporarily attached to the resin seal ring 22.
In this state, the resin seal ring 22 is placed and fitted on the funnel placing portion 25 of the housing 23. At this time, only the membrane filter 30 is removed from FIG. 5 or the membrane filter 30 and the filter paper 31 are removed.
Both have been removed.

After the assembly is sterilized by an autoclave, the resin seal ring 22 and the paper funnel 3 are sterilized.
3 is temporarily removed from the housing 23, and the membrane filter 30 is placed on the filter paper 31 exposed by this (the filter paper 31 and the membrane filter 30 are placed on the projection 32 when the filter paper 31 is not placed on the projection 32), etc. Place using. Then, the resin seal ring 22 once removed is mounted and fitted on the funnel mounting portion 25 of the housing 23 with the paper funnel 33 mounted thereon, thereby obtaining the microorganism collection kit of FIG. Is obtained. The membrane filter 30 is sterilized in advance and hermetically stored in a bag.

Since the bottom portion 37 of the resin seal ring 22 extends inward from the funnel mounting portion 25 of the housing 23 so as to extend above the filter housing portion 24,
The membrane filter 3 housed in the filter housing 24
0 can be prevented from rising by pressing the outer peripheral portion. In order to enhance this effect, a downward projection 41 may be provided on the inner end of the bottom portion 37 (see FIG. 7).

The bottom edge 3 of the paper funnel 33
It is preferable to provide a recess 42 at the bottom of the insertion groove 40 in order to facilitate the insertion of the groove 6 (see FIG. 7).

A protrusion 43 is provided on the inner peripheral side of the upper end of the housing peripheral wall 26 (see FIG. 5), and the outer peripheral wall 3 of the resin seal ring 22 mounted on the funnel mounting portion 25 is provided.
9 is engaged with the resin seal ring 22.
Work as a retainer. The protrusions 43 may be formed over the entire circumference, but it is preferable to provide several protrusions at appropriate regular intervals (for example, 120 ° intervals) so as not to hinder the attachment of the resin seal ring 22.

A protruding piece 44 may be formed by extending a part of the upper end of the housing peripheral wall 26 outward (see FIG. 8).
By holding the projecting piece 44 by hand, the resin seal ring 2
2 can be easily removed.

In the above-mentioned kit, since the housing 23 and the resin seal ring 22 formed of a resin material capable of being autoclaved and sterilized are mutually detachable, autoclave sterilization is performed even after use once. Can be reused, and the total cost required for the microbial test performed many times can be reduced.

Although the preferred embodiments of the present invention have been described with reference to the drawings, the present invention is not limited to these illustrated embodiments, but is defined by the appended claims. Various modifications are possible within the scope of the invention.

For example, in the embodiment shown in FIG. 5, the membrane filter 30 is superposed on the filter
Although mounted above, it is within the scope of the present invention to use the membrane filter 30 alone without using the filter paper 31. However, since the membrane filter 30 is generally expensive, it is advantageous in terms of cost to use a filter that is as thin as possible, and it is preferable to use a filter paper 31 that can be obtained relatively inexpensively. Further, there is an advantage that it can be applied to a usage method in which a liquid medium is soaked in the filter paper 31 for use.

[0066]

According to the present invention, the use of paper funnels makes it possible to make the microorganism collecting apparatus inexpensive enough for disposable use, and to reduce the leakage of the specimen which is a problem in that case. Therefore, it is extremely practical as a disposable type microorganism collecting apparatus.

The microorganism collecting apparatus of the present invention can be applied not only to the examination of microorganisms in a solution but also to any kind of fluid. For example, when the cleanliness is measured by examining the number of bacteria in the air. Can also be used effectively.

[Brief description of the drawings]

FIG. 1 is a front half sectional view showing the configuration of a microorganism collection kit according to one embodiment of the present invention.

FIG. 2 is a sectional view taken along the line AA in FIG.

FIG. 3 is a top view showing an example of a shape of a resin seal ring that can be used by being fitted into a gap between double wall structures of a housing in the microorganism collection kit of FIG. 1;

FIG. 4 is an enlarged sectional view taken along line BB in FIG. 3;

FIG. 5 is a front half sectional view showing a configuration of a microorganism collection kit according to an embodiment of the present invention using a resin seal ring.

FIG. 6 is a top view of a resin seal ring used in the microorganism collection kit of FIG. 5;

FIG. 7 is an enlarged sectional view taken along the line CC in FIG. 6;

FIG. 8 is a top view showing a modification of the housing shape.

[Explanation of symbols]

DESCRIPTION OF SYMBOLS 1 Housing 2 Housing filter accommodation part 3 Membrane filter 4 Packing 5 Housing lower accommodation part 6 Porous material 7 Housing outlet 8 Radial rib (protrusion) 9 Housing double wall structure part 9a Double wall structure part Inner wall 9b Outer wall of double wall structure portion 10 Void portion of double wall structure portion 11 Resin sealant 12 Paper funnel 13 Peripheral side surface of paper funnel 14 Bottom surface of paper funnel functioning as prefilter 15 Peripheral edge of paper funnel Part 16 Resin seal ring 17 Concave groove of resin seal ring 18 Extension part of resin seal ring 19 Projection on inner peripheral side of resin seal ring 20 Projection on outer peripheral side of resin seal ring 21 Insertion groove of resin seal ring 22 Resin seal ring 23 Housing 24 Housing filter housing 25 Housing housing Flannel mounting portion 26 Peripheral wall of housing 27 Concave recess in filter housing portion of housing 28 Outlet of housing 29 Radial rib 30 Membrane filter 31 Filter paper 32 Ridge 33 Paper funnel 34 Peripheral side surface of paper funnel 35 Bottom surface of paper funnel 36 Paper Peripheral part of funnel made of resin 37 Bottom part of resin seal ring 38 Inner peripheral wall of resin seal ring 39 Outer peripheral wall of resin seal ring 40 Insertion groove of resin seal ring 41 Projection of resin seal ring bottom part 42 Recessed recess of resin seal ring insertion groove 43 Inward protrusion at upper end of housing peripheral wall 44 Outer extension projecting piece at upper end of housing peripheral wall 45 Radial rib

Claims (17)

[Claims] An integrated unit having a filter accommodating portion, a funnel mounting portion provided outside the filter accommodating portion at a level slightly higher than the filter accommodating portion, and a discharge port opened in the filter accommodating portion. A resin molded housing,
A membrane filter accommodated in the filter accommodating portion of the housing for collecting target microorganisms in the sample, and a bottom peripheral portion thereof is mounted on the funnel mounting portion of the housing via a resin seal means in a liquid-tight manner. And a paper funnel whose bottom surface functions as a pre-filter for collecting microorganisms and food residues whose particle size is smaller than the target microorganism in the sample. Microorganism collection device. 2. The filter according to claim 1, wherein a porous material is accommodated in a lower accommodating portion recessed in the filter accommodating portion, and the membrane filter is placed and supported on the porous material. Microorganism collection device. 3. The housing according to claim 1, wherein projections are provided at intervals on the bottom surface of the lower storage portion of the housing, and the porous material is placed on the projections and stored in the lower storage portion. Item 3. The microorganism collecting device according to Item 2. 4. The microorganism collecting apparatus according to claim 2, wherein the porous material accommodated in the lower accommodation portion of the housing is PP (polypropylene) or filter paper. 5. A filter housing, a double-walled structure provided at a level slightly higher than the filter housing outside the filter housing, and a lower housing recessed in the filter housing. A housing integrally formed of resin having an outlet opening to the lower housing portion, a membrane filter housed in a filter housing portion of the housing for collecting target microorganisms in a specimen, A paper cup-shaped funnel whose bottom edge is inserted into the gap between the double-walled structures and is fixed in a liquid-tight manner by a resin sealant filled in the gap, and the bottom of which is formed in the specimen. A paper funnel that functions as a pre-filter that collects microorganisms and food residues that are coarser in particle size than the target microorganism, and a lower part of the housing to support the membrane filter from below And a porous material received in the receiving section, a microorganism collecting apparatus characterized by including the. 6. The microorganism collecting apparatus according to claim 5, wherein a substantially ring-shaped packing for further pressing the membrane filter from above is detachably provided in the filter accommodating portion of the housing. 7. The microorganism collecting apparatus according to claim 6, wherein the membrane filter is accommodated in a filter accommodating portion of the housing while being fixed to a bottom of the packing. 8. The microorganism collecting apparatus according to claim 5, wherein the resin sealant filled in the gap between the double wall structure portions of the housing is silicon. 9. A filter accommodating portion, a funnel mounting portion provided at a level slightly higher than the filter accommodating portion outside the filter accommodating portion, a peripheral wall surrounding the funnel mounting portion, and a filter accommodating portion. A housing integrally formed of resin having an opening to be opened, a membrane filter housed in a filter housing portion of the housing for collecting target microorganisms in the sample, and a particle size of the target microorganisms in the sample. A paper funnel having a bottom surface functioning as a pre-filter for collecting coarse microorganisms and food residues, and the paper funnel is placed on the funnel placement portion of the housing with the bottom edge of the paper funnel fitted. And a resin seal ring fitted into the peripheral wall to attach the paper funnel in a liquid-tight manner. 10. The microorganism collecting apparatus according to claim 9, wherein said resin seal ring is detachable from both said paper funnel and said housing. 11. A membrane accommodated in the filter accommodating portion with a bottom surface of the resin seal ring extending from the funnel mounting portion of the housing to a position above the filter accommodating portion. 11. The microorganism collecting apparatus according to claim 9, wherein an outer peripheral portion of the filter is pressed. 12. An outer peripheral edge of the resin seal ring has a double wall structure, and a bottom peripheral edge of the paper funnel is fitted in a gap between the double wall structures. The outer wall surface of the heavy wall structure portion is attached in a state in which the outer wall surface is in close contact with the inner surface of the peripheral wall of the housing.
The microorganism collection device according to any one of claims 1 to 7. 13. The microorganism collecting apparatus according to claim 9, wherein said resin seal ring is made of silicon. 14. A housing according to claim 9, wherein a protrusion protruding inward is provided at an upper end of the peripheral wall of the housing, and the protrusion presses an upper end of the resin seal ring to prevent the resin seal ring from floating. The microorganism collecting device according to the above. 15. The microorganism collecting apparatus according to claim 9, wherein a part of the upper end of the peripheral wall of the housing projects outward to form a projecting piece. 16. The microorganism collecting apparatus according to claim 9, wherein said membrane filter is accommodated in a filter accommodating portion of said housing in a state of being superposed on filter paper. 17. A plurality of radial protrusions are provided at intervals in a concave portion provided in the filter accommodating portion, and the membrane filter is placed on the radial protrusions in a state of being superimposed on the filter paper. 17. The microorganism collecting apparatus according to claim 16, wherein: