CN216703486U

CN216703486U - Filter equipment of endophytic fungi zymotic fluid

Info

Publication number: CN216703486U
Application number: CN202122432235.6U
Authority: CN
Inventors: 杨云; 官会林; 黄晶心; 郭嘉航; 卢里芬; 钟正阳
Original assignee: Yunnan Normal University
Current assignee: Yunnan Normal University
Priority date: 2021-10-10
Filing date: 2021-10-10
Publication date: 2022-06-10
Anticipated expiration: 2031-10-10

Abstract

The utility model discloses a filter device for endophytic fungi fermentation liquor, which comprises a fixed support, an adjustable fixed clamp arranged on the fixed support, a three-section type filter cylinder, a cover at the top end of the filter cylinder, a flat-bottom type porous filter cylinder arranged at the first section, filter paper arranged on the flat-bottom type filter cylinder, a filter cylinder arranged at the second section, a vacuum pump connected with the filter cylinder, a turnover interlayer plate arranged in the two-section filter cylinder, a filter cylinder arranged at the position of the filter cylinder, a filter membrane arranged at the position of an opening of the filter cylinder, a flow guide gate valve connected with the filter cylinder, a piece-inserted filter membrane arranged below the gate valve and a container for containing filtrate. The method is used for separating mycelium, spores and filtrate of endophytic fungi fermentation filtrate, and solves the problems of pollution of mixed fungi in the filtering process and easy blockage of spores and low filtering efficiency when a water system filter membrane of a filtrate syringe is filtered. The device has the advantages of simple structure, easy operation, low cost, obvious effect, no need of post-processing and strong practicability.

Description

Filter equipment of endophytic fungi zymotic fluid

Technical Field

The utility model belongs to the technical field of microbial filtration, and particularly relates to a filter device for endophytic fungi fermentation liquor.

Background

In the development of the biological activity research of endophytic fungi metabolites, endophytic fungi fermentation liquor is required to be prepared, the fermentation liquor is often easily polluted by other mixed bacteria during filtration, and meanwhile, when an injector is used for filtration through a water system filter membrane, a filter head is often easily blocked by spores under filtration, so that the filtration difficulty is increased. Therefore, the simple filter device for the endophytic fungi fermentation liquor is designed, and the filter device can effectively prevent the mixed fungi pollution and improve the filtering efficiency.

SUMMERY OF THE UTILITY MODEL

Aiming at the problems that the endophytic fungi fermentation liquor is easily polluted by mixed bacteria and the filtering efficiency is low during filtering, the utility model designs a filtering device for the endophytic fungi fermentation liquor.

The technical scheme of the utility model is as follows:

a filter device for endophytic fungi fermentation liquor comprises a main body and a filter unit, wherein the main body comprises a fixed support, an adjustable fixing clamp arranged on the fixed support, a three-section type filter cylinder, a cover at the top end of the filter cylinder, a flat-bottom type porous filter cylinder with one section, filter paper arranged on the flat-bottom type filter cylinder, a pumping filter cylinder with the two section, a vacuum pump connected with the pumping filter cylinder, a turnover interlayer plate arranged in the two section filter cylinder, a nested bevel filter cylinder with three sections, nested filter membranes arranged at the mouth of the three section filter cylinder, a diversion gate valve connected with the filter cylinder, a plug-in type filter membrane arranged below the gate valve and a container for containing filtrate.

Furthermore, the three-section type filter cylinder is made of glass, and the cover of the filter cylinder is a rotatable and fixed cover made of polyethylene, so that the filter cylinder is convenient to take down and cover. The additional installation of the cover can effectively prevent the pollution of external mixed bacteria to the filtrate during the filtration.

Furthermore, the filter paper used in the section of filter cylinder is qualitative filter paper, the qualitative filter paper is low in cost, hypha and filtrate can be separated, 2 layers are required to be placed during filtering, and the filter paper is prevented from being broken during suction filtration.

Furthermore, the overturning interlayer plate in the two-section filter cylinder has the effect of separating the two-section filter cylinder from the three-section filter cylinder, the used manufacturing material is hard plastic, and the periphery of the interlayer is provided with a rubber sleeve; when the partition plate is closed, the filter can be completely sealed, vacuum filtration negative pressure is formed, filtering is more convenient, and filtrate can be discharged into the three-section filter drum by rotating the cover plate after the filtrate is filtered.

Furthermore, the lower end opening of the two-section filter cylinder is smaller than the upper end opening of the three-section filter cylinder, so that the two-section filter cylinder is convenient to nest; after the filter cylinders are nested, the upper filter cylinder interface and the lower filter cylinder interface are fixed through the convex part of the outer wall by using a spring pinching clip made of metal.

Furthermore, the inner rings at the openings of the three sections of filter cylinders are slightly protruded, and discs for holding the filter membranes can be contained.

Furthermore, the three-section filter cylinder is designed to be an inclined plane, which is beneficial to accelerating the flow speed of filtration.

Further, the filter membrane used in the two-stage and three-stage cartridges was a 0.22 μm aqueous filter membrane.

The utility model has the beneficial effects that:

the utility model provides a filter device for endophytic fungi fermentation liquor, which is used for separating mycelium from filtrate in the fermentation liquor and can effectively reduce the pollution of mixed bacteria in the filtering process and the problems of easy spore blockage and low filtering efficiency when a filtrate syringe water system filter membrane is filtered. The device has the advantages of simple structure, easy operation, low cost, obvious effect, no need of post-processing and strong practicability.

Drawings

FIG. 1 is a schematic view of the structure of a filtration apparatus for fermentation broth of endophytic fungi of the present invention.

The filter comprises a fixing support 1, an adjustable fixing clamp 2, a filter cylinder top cover 3, a flat-bottom type porous filter cylinder 4, filter paper 5, a filter cylinder 6, a turnover division plate 7, a turnover rotary button 8, a nested water system filter membrane 9, a spring nip clamp 10, a filter cylinder 11, a slope filter cylinder 11, a flow guide gate valve 12, a plug-in type water system filter membrane 13, a filtrate collecting bottle 14, a suction filtration cylinder air outlet pipe 15 and a vacuum pump 16.

Detailed Description

The technical solution of the present invention is explained in detail by the following specific examples.

Example 1

As shown in FIG. 1, FIG. 1 is a schematic structural diagram of a filtration apparatus for endophytic fungi fermentation broth.

The utility model relates to a filter device for endophytic fungi fermentation liquor, which comprises a fixed support 1, an adjustable fixed clamp 2, a rotary fixed filter cylinder cover 3, a flat-bottom porous filter cylinder 4, filter paper 5 for suction filtration, a two-section suction filter cylinder 6, a turnover type interlayer plate 7, a turnover rotary button 8, a nested water system filter membrane 9 for filtering spores, a two-section and three-section filter cylinder fixing spring nip clamp 10, a three-section inclined surface filter cylinder 11, a diversion gate valve 12, an insert type water system filter membrane 13, a filtrate collecting bottle 14, an air outlet pipe 15 of the suction filter cylinder and a vacuum pump 16 connected with the air outlet pipe of the suction filter cylinder.

When in use, the turnover type partition plate 7 and the diversion gate valve 12 are in a closed state, the rotary type fixed cover 3 is opened, 2 layers of qualitative filter paper are put into a section of flat-bottom type porous filter cylinder, fermentation liquor is poured into the flat-bottom type porous filter cylinder, and the rotary type fixed cover 3 is covered; opening the vacuum pump 16, and closing the vacuum pump 16 after the filtrate is filtered; opening the turnover type interlayer plate 7, and closing the turnover rotary button 8 after filtering the filtrate; and (4) opening the diversion gate valve 12, collecting the filtrate by using a filtrate collecting bottle 14, and closing the diversion gate valve 12 after the filtration is finished. Opening the rotary fixed cover 3 to take out the separated hyphae, opening the fixed spring nip clamp 10, and collecting the spores obtained by filtration on the water system filter membrane.

Various equivalent modifications and substitutions may be made by those skilled in the art according to the experimental needs without departing from the functional and spirit of the utility model, and such modifications and substitutions are intended to be within the scope of the utility model. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.

Claims

1. A filter device for endophytic fungi fermentation liquor comprises a fixed support (1), a vacuum pump (16) arranged on the fixed support (1), a three-section type filter cylinder (a flat-bottom porous filter cylinder (4), a suction filtration cylinder (6), an inclined filter cylinder (11)), and a filtrate collection bottle (14); the method is characterized in that: an adjustable fixing clamp (2) is arranged on a support rod of the fixing support (1), a cover (3) is arranged at the top end of a flat-bottom type porous filter cylinder (4) placed on the fixing clamp (2), filter paper (5) is placed in the flat-bottom type porous filter cylinder (4), a filter cylinder (6) is connected with a filter cylinder air outlet pipe (15), the filter cylinder air outlet pipe (15) is connected with a vacuum pump (16) placed on the fixing support (1), an overturning interlayer plate (7) is arranged in the filter cylinder (6), the overturning interlayer plate (7) is connected with an overturning rotating button (8), the filter cylinder (6) is connected with an inclined filter cylinder (11), the outer wall of the joint is fixed by a spring caulking clamp (10), and a nested water system filter membrane (9) is placed inside a position 2cm below the opening end of the inclined filter cylinder (11), the lower end of the inclined surface filter cylinder (11) is provided with a flow guide gate valve (12), the lower end of the flow guide gate valve (12) is provided with a plug-in sheet type water system filter membrane (13), and the filtrate collecting bottle (14) is used for containing filtrate flowing out of the inclined surface filter cylinder (11).

2. The apparatus of claim 1, wherein the apparatus comprises: the cover (3) at the top end of the flat-bottom type porous filter cylinder (4) is a rotary cover.

3. The apparatus of claim 1, wherein the apparatus comprises: the filter paper (5) used in the flat-bottom type porous filter cylinder (4) is qualitative filter paper.

4. The apparatus of claim 1, wherein the apparatus comprises: the interlayer plate (7) in the pumping filter cylinder (6) can be turned over, and the edge of the interlayer plate is provided with a rubber sleeve.

5. The apparatus of claim 1, wherein the apparatus comprises: the inner wall of the position 3cm upwards from the opening at the lower end of the pumping filter cylinder (6) is provided with a raised spiral ring.

6. The apparatus of claim 1, wherein the apparatus comprises: the part, 3cm downwards, of the upper end opening of the inclined surface filter cylinder (11) is an inner concave spiral outer wall, and a circle of convex wall is arranged on the inner wall, 2cm downwards, of the upper end opening of the inclined surface filter cylinder (11) and used for placing the nested water system filter membrane (9).

7. The apparatus of claim 1, wherein the apparatus comprises: the filter membrane in the nested water system filter membrane (9) can be detached, and the used filter membrane is a 0.22 mu m water system filter membrane.

8. The apparatus of claim 1, wherein the apparatus comprises: the filter membrane used for the insert type water system filter membrane (13) is a 0.22 μm water system filter membrane.