CN109266529A - A kind of cell filter structure - Google Patents
A kind of cell filter structure Download PDFInfo
- Publication number
- CN109266529A CN109266529A CN201811181206.3A CN201811181206A CN109266529A CN 109266529 A CN109266529 A CN 109266529A CN 201811181206 A CN201811181206 A CN 201811181206A CN 109266529 A CN109266529 A CN 109266529A
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- China
- Prior art keywords
- filter
- sample volume
- filter membrane
- volume pipe
- mounting structures
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
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- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Sustainable Development (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
Abstract
The invention discloses a kind of cell filter structures, comprising: sample volume pipe, filter membrane mounting structures, filter membrane, top plug, lower plug, screw thread cover-buckling structure, wherein sample volume pipe upper end opening, top plug are arranged in its opening；Sample volume pipe lower end is provided with a filter membrane, and filter membrane mounting structures are arranged below filter membrane；It is provided with the notch to match on filter membrane mounting structures and sample volume pipe, the two is attached by notch, which includes at least one slot；Filter membrane mounting structures lower part is provided with filter drain interface, and lower plug is arranged in the filter drain interface, and screw thread buckle closure structure setting is fixed by way of screw thread in sample volume pipe lower part；It is connected and sealed by the way of the sealing means of side between filter membrane mounting structures and sample volume pipe；It is connected and sealed by the way of the sealing means of side between filter drain interface and filter membrane mounting structures.The cell filter stable structure, it is reliable, be easy to use.
Description
Technical field
The present invention relates to field of biotechnology, particularly relate to a kind of cell filter structure.
Background technique
Circulating tumor cell (circulating tumor cells, CTCs) refers to spontaneous or because operation of diagnosis and treatment is by entity
Tumor or transfer stove discharge into the tumour cell of Peripheral Circulation, are that malignant tumor patient postoperative recurrence and DISTANT METASTASES IN occurs
Major reason.In recent years, continuously improving with detection technique, CTCs detection is as a kind of novel non-invasive diagnostic work
Tool, early detection patient postoperative recurrence and DISTANT METASTASES IN, assessment curative effect in terms of application value have become clinic
The hot spot of research.
Since quantity of the CTCs in peripheral blood is few, need to usually be sought in about 100,000,000 leucocytes and 50,000,000,000 red blood cells
Only several tumour cells are looked for, therefore in order to improve the recall rate of CTCs, row CTCs need to usually be enriched with, i.e., will before detection
CTCs is separated from blood or other samples.The enrichment method of CTCs is broadly divided into immunomagnetic isolation by its principle at present
The concentration method of method and the physical property based on CTCs.Wherein, the concentration method of the physical property based on CTCs is mainly by density gradient
Centrifugal process and filtration method.
Filtration method is partition method (the isolation by size of epithelial based on tumour cell size
Tumor cells, ISET), tumour cell is mainly separated according to the of different sizes of tumour cell and normal cell.It is this to pass through
The cellular morphology of physical method for separation saves completely, and the various antigens or molecular labeling on surface do not influence cell without destruction
Characteristic provides good condition for subsequent detection, and this method equipment and technology is of less demanding, and process is easy to grasp.
But rotation of the cell filter in the prior art in dismounting or use process is easy to impact filter membrane,
The prior art lacks effective solution mode.Therefore, the present inventor needs to conceive a kind of new technology to improve its problem.
Summary of the invention
In order to solve the above technical problems, stablizing the present invention provides a kind of cell filter structure, being reliable, being easy to make
With.
The technical scheme is that
A kind of cell filter structure, comprising: sample volume pipe, filter membrane mounting structures, filter membrane, top plug, lower plug, screw buckle
Lid structure, wherein the sample volume pipe upper end opening, the top plug is arranged in its opening；Sample volume pipe lower end is set
It is equipped with a filter membrane, the filter membrane mounting structures are arranged below the filter membrane；The filter membrane mounting structures and the sample volume
The notch to match is provided on pipe, the two is attached by notch, which includes at least one
Slot；Filter membrane mounting structures lower part is provided with filter drain interface, and the lower plug is arranged in the filter drain interface, institute
Screw thread buckle closure structure setting is stated in sample volume pipe lower part, and is fixed by way of screw thread；The filter membrane support
It is connected and sealed by the way of the sealing means of side between structure and the sample volume pipe；The filter drain interface with
It is connected and sealed by the way of the sealing means of side between the filter membrane mounting structures.
Preferably, it is provided with liquid pouring flange at the sample volume pipe upper end-face edge, when for avoiding liquid from pouring out
Outer tube wall along the sample volume pipe flows out.
Preferably, scale marker is provided on the sample volume pipe lateral wall.
Preferably, the side sealing means include but is not limited to the sealing of outer ring O-ring, outer ring washer sealing or outer loop knot
Structure directly contacts sealing.
Preferably, the notch is annular slot structure.
Preferably, the top plug is silica gel top plug.
Preferably, the lower plug is to fill under silica gel.
Preferably, the high outlet pipe side wall 0.2mm or more of the liquid pouring flange of the sample volume pipe.
By adopting the above technical scheme, the present invention include at least it is following the utility model has the advantages that
Cell filter structure of the present invention is stablized, is reliable, being easy to use.Side is used inside cell filter
Sealing means reduce the stress to filter membrane while reaching sealing effect, while providing reliable sealing.Make inside cell filter
It effectively prevent cell filter to occur Effect of Rotation filter membrane in dismounting or use with notch.In addition sample volume pipe outer wall
Scale marker can quickly read remaining liq capacity in pipe.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of cell filter structure of the present invention；
Fig. 2 is the cross-sectional view of cell filter structure of the present invention；
Fig. 3 is the fractionation schematic diagram of cell filter structure of the present invention.
Wherein: 1. top plugs, 2. liquid pouring flange, 3. sample volume pipes, 4. scale markers, 5. filter membranes, 6. washers, 7. spiral shells
Line cover-buckling structure, 8. lower plugs, 9.O type circle, 10. notchs, 11. internal screw threads, 12. external screw threads.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
As shown in Figure 1 to Figure 3, to meet a kind of cell filter structure of the invention, comprising: sample volume pipe 3, filter membrane
5 mounting structures, filter membrane 5, top plug 1, lower plug 8, screw thread cover-buckling structure 7, wherein 3 upper end opening of sample volume pipe, it is described on
Plug 1 is arranged in its opening；3 lower end of sample volume pipe is provided with a filter membrane 5, and 5 mounting structures of filter membrane are arranged in institute
State 5 lower section of filter membrane；It is provided with the notch 10 to match on 5 mounting structures of filter membrane and the sample volume pipe 3, two
Person is attached by notch 10, which includes at least one slot；The 5 mounting structures lower part of filter membrane is set
It is equipped with filter drain interface, in the filter drain interface, the screw thread cover-buckling structure 7 is arranged described for lower 8 setting of plug
3 lower part of sample volume pipe, and it is fixed by way of screw thread that (internal screw thread 11 and external screw thread 12 as shown in Figure 3 is matched
It closes)；It is connected and sealed by the way of the sealing means of side between 5 mounting structures of filter membrane and the sample volume pipe 3；
It is connected and sealed by the way of the sealing means of side between 5 mounting structures of the filter drain interface and the filter membrane, such as
It is shown in Fig. 2 to be sealed by O-ring 9.
Preferably, it is provided with liquid pouring flange 2 at 3 upper end-face edge of sample volume pipe, for avoiding liquid from pouring out
When along the sample volume pipe 3 outer tube wall flow out.
Preferably, scale marker 4 is provided on 3 lateral wall of sample volume pipe.
Preferably, the side sealing means include but is not limited to the sealing of outer ring O-ring, the sealing of outer ring washer 6 or outer ring
Structure directly contacts sealing.
Preferably, the notch 10 is annular slot structure 10.
Preferably, the top plug 1 is silica gel top plug 1.
Preferably, the lower plug 8 is to fill in 8 under silica gel.
Preferably, the liquid pouring flange 2 of the sample volume pipe 3 is outlet pipe side wall 0.2mm or more high.
A kind of structure of cell filter is present embodiments provided, to avoid cell filter in dismounting or use process
Rotation filter membrane 5 is impacted, cell filter structure holds sample volume pipe 3 with filter membrane 5 using annular slot structure 10
Holder structure is fixed after being attached with the mode of screw thread, is sealed between 5 mounting structures of volume tube and filter membrane using side, filter
The positive outer ring auxiliary seal of film 5.Between cell filter and filter drain interface using outer ring side seal, cell filter with
It is sealed between interface using side.Positive pressure and negative pressure mode can be used equipped with filling in 8 under silica gel top plug 1 and silica gel in cell filter
It is tested.Cell filter sample volume pipe 3 has scale marker 4, can directly read liquid in pipe volume.Cell filter sample
3 upper end-face edge of this volume tube liquid when liquid being avoided to pour out that turns up is flowed out with outer tube wall.
Side main seal, the mode of front auxiliary seal are used in the embodiment.As shown in Fig. 2, liquid pouring turns over
Liquid is flowed down with pipe outer wall when pouring liquid can be effectively prevented in side 2.Reached inside cell filter using side sealing means
Reduce the stress to filter membrane 5 while sealing effect, while reliable sealing being provided.Shown in Fig. 3, card is used inside cell filter
Slot structure 10 effectively prevent cell filter to occur Effect of Rotation filter membrane 5 in dismounting or use.The quarter of 3 outer wall of sample volume pipe
Scale, which knows 4, can quickly read remaining liq capacity in pipe.
The foregoing description of the disclosed embodiments enables those skilled in the art to implement or use the present invention.
Various modifications to these embodiments will be readily apparent to those skilled in the art, as defined herein
General Principle can be realized in other embodiments without departing from the spirit or scope of the present invention.Therefore, of the invention
It is not intended to be limited to the embodiments shown herein, and is to fit to and the principles and novel features disclosed herein phase one
The widest scope of cause.
Claims (8)
1. a kind of cell filter structure characterized by comprising sample volume pipe, filter membrane mounting structures, filter membrane, top plug, under
Plug, screw thread cover-buckling structure, wherein the sample volume pipe upper end opening, the top plug is arranged in its opening；The sample holds
Product pipe lower end is provided with a filter membrane, and the filter membrane mounting structures are arranged below the filter membrane；The filter membrane mounting structures and institute
The notch for being provided with and matching on sample volume pipe is stated, the two is attached by notch, the notch packet
Include at least one slot；Filter membrane mounting structures lower part is provided with filter drain interface, and the lower plug setting is arranged in the filter
At liquid interface, the screw thread buckle closure structure setting is fixed by way of screw thread in sample volume pipe lower part；Institute
It states and is connected and sealed by the way of the sealing means of side between filter membrane mounting structures and the sample volume pipe；The filter
It is connected and sealed by the way of the sealing means of side between drain interface and the filter membrane mounting structures.
2. cell filter structure as described in claim 1, it is characterised in that: be arranged at the sample volume pipe upper end-face edge
There is liquid pouring flange, being flowed out when for avoiding liquid from pouring out along the outer tube wall of the sample volume pipe.
3. cell filter structure as claimed in claim 1 or 2, it is characterised in that: set on the sample volume pipe lateral wall
It is equipped with scale marker.
4. cell filter structure a method according to any one of claims 1-3, it is characterised in that: the side sealing means include but
It is not limited to the sealing of outer ring O-ring, outer ring washer sealing or outer ring structure and directly contacts sealing.
5. the cell filter structure as described in claim 1-4 is any, it is characterised in that: the notch is annular slot
Structure.
6. cell filter structure a method as claimed in any one of claims 1 to 5, it is characterised in that: the top plug is silica gel top plug.
7. the cell filter structure as described in claim 1-6 is any, it is characterised in that: the lower plug is to fill under silica gel.
8. the cell filter structure stated such as claim 2, it is characterised in that: the liquid pouring flange of the sample volume pipe is high
Outlet pipe side wall 0.2mm or more.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811181206.3A CN109266529A (en) | 2018-10-11 | 2018-10-11 | A kind of cell filter structure |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811181206.3A CN109266529A (en) | 2018-10-11 | 2018-10-11 | A kind of cell filter structure |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109266529A true CN109266529A (en) | 2019-01-25 |
Family
ID=65196342
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811181206.3A Pending CN109266529A (en) | 2018-10-11 | 2018-10-11 | A kind of cell filter structure |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109266529A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112126588A (en) * | 2019-06-24 | 2020-12-25 | 泰克瑞普私人有限责任公司 | Separation capture system for specific cells in liquid sample |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4678576A (en) * | 1981-09-16 | 1987-07-07 | Nalge Company | Reusable filter unit with recoverable filter membrane |
| CN1896223A (en) * | 2006-06-30 | 2007-01-17 | 沈佳特 | Disposable and closed aseptic thin-film filtering culturing dish |
| CN103599574A (en) * | 2013-11-22 | 2014-02-26 | 武汉友芝友医疗科技有限公司 | Filter for isolating circulating tumor cells |
| CN104498348A (en) * | 2014-12-19 | 2015-04-08 | 武汉友芝友医疗科技有限公司 | Filter used for separating specific cells |
| CN108325388A (en) * | 2018-04-18 | 2018-07-27 | 武汉芯生生物科技有限公司 | A kind of filter for detaching circulating tumor cell |
-
2018
- 2018-10-11 CN CN201811181206.3A patent/CN109266529A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4678576A (en) * | 1981-09-16 | 1987-07-07 | Nalge Company | Reusable filter unit with recoverable filter membrane |
| CN1896223A (en) * | 2006-06-30 | 2007-01-17 | 沈佳特 | Disposable and closed aseptic thin-film filtering culturing dish |
| CN103599574A (en) * | 2013-11-22 | 2014-02-26 | 武汉友芝友医疗科技有限公司 | Filter for isolating circulating tumor cells |
| CN104498348A (en) * | 2014-12-19 | 2015-04-08 | 武汉友芝友医疗科技有限公司 | Filter used for separating specific cells |
| CN108325388A (en) * | 2018-04-18 | 2018-07-27 | 武汉芯生生物科技有限公司 | A kind of filter for detaching circulating tumor cell |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112126588A (en) * | 2019-06-24 | 2020-12-25 | 泰克瑞普私人有限责任公司 | Separation capture system for specific cells in liquid sample |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
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| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190125 |