CN213357583U - Aseptic sampling device of cell culture liquid - Google Patents
Aseptic sampling device of cell culture liquid Download PDFInfo
- Publication number
- CN213357583U CN213357583U CN202021813903.9U CN202021813903U CN213357583U CN 213357583 U CN213357583 U CN 213357583U CN 202021813903 U CN202021813903 U CN 202021813903U CN 213357583 U CN213357583 U CN 213357583U
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- separation mechanism
- buffer memory
- hose
- screw rod
- cell culture
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- 239000007788 liquid Substances 0.000 title claims abstract description 22
- 238000005070 sampling Methods 0.000 title claims abstract description 18
- 238000004113 cell culture Methods 0.000 title claims abstract description 14
- 230000007246 mechanism Effects 0.000 claims abstract description 42
- 238000000926 separation method Methods 0.000 claims abstract description 34
- 238000007789 sealing Methods 0.000 claims description 4
- 239000012930 cell culture fluid Substances 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 238000005192 partition Methods 0.000 description 6
- 230000000903 blocking effect Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004166 bioassay Methods 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- -1 trace elements) Chemical class 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses an aseptic sampling device of cell culture liquid, including the buffer memory jar, buffer memory bottom of the tank end is connected with first separation mechanism, first separation mechanism bottom is connected with the connecting pipe, the sampling needle is installed to the connecting pipe bottom, the buffer memory tank top is provided with the closing cap, be provided with suction means on the closing cap, the one end of buffer memory jar is connected with the hose, the one end that buffer memory jar was kept away from to the hose is connected with second separation mechanism, the one end that the hose was kept away from to second separation mechanism is connected with the drain pipe, the utility model discloses overall structure is simple, and easily manufacturing realizes, and low cost can satisfy the demand of the aseptic sample of culture liquid, convenient to use.
Description
Technical Field
The utility model relates to a biological assay apparatus technical field specifically is a sterile sampling device of cell culture liquid.
Background
Culture broth is an artificially prepared nutrient for the growth and maintenance of microorganisms, plant and animal tissues, and generally contains carbohydrates, nitrogen-containing substances, inorganic salts (including trace elements), vitamins, water, and the like. Cell culture solutions are often prepared in the laboratory for various studies. In the research process of the cell culture solution, sampling operation is often required, at present, sampling is mostly carried out through simple instruments such as a dropper and the like, and the cell culture solution is difficult to ensure that the cell culture solution is not polluted by external bacteria in the sampling process.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide an aseptic sampling device of cell culture liquid to solve the problem that proposes in the above-mentioned background art.
In order to achieve the above object, the utility model provides a following technical scheme:
the utility model provides an aseptic sampling device of cell culture liquid, includes the buffer memory jar, buffer memory bottom of the tank end is connected with first separation mechanism, first separation mechanism bottom is connected with the connecting pipe, the sample needle is installed to the connecting pipe bottom, the buffer memory top of the tank end is provided with the closing cap, be provided with suction mechanism on the closing cap, the one end of buffer memory jar is connected with the hose, the one end that buffer memory jar was kept away from to the hose is connected with second separation mechanism, the one end that hose was kept away from to second separation mechanism is connected with the drain pipe.
Preferably, the side wall surface of the buffer tank is provided with a transparent observation window.
Preferably, the surface of the observation window is provided with a graduated scale.
Preferably, the suction mechanism comprises a screw rod, a threaded hole corresponding to the screw rod is formed in the surface of the sealing cover, a knob is installed at the top end of the screw rod, and the bottom end of the screw rod extends to the inside of the buffer tank and is rotatably connected with a piston.
Preferably, first separation mechanism and second separation mechanism structure are the same, all include the casing, the one end of casing is provided with the input tube, and the other end is provided with the output tube, the inside baffle that is provided with of casing, a plurality of through-holes have been seted up on the baffle surface, there is the valve ball baffle towards the one end of input tube through spring coupling, the diameter of valve ball is greater than the pipe diameter of input tube.
Preferably, the input pipe of the first separation mechanism is connected with the connecting pipe, the output pipe is connected with the cache tank, the input pipe of the second separation mechanism is connected with the hose, and the output pipe is connected with the liquid outlet pipe.
Compared with the prior art, the beneficial effects of the utility model are that: the arrangement of the observation window and the graduated scale is convenient for observing the liquid capacity in the buffer tank; when liquid is input from the input pipe, the pressure overcomes the elasticity of the spring to enable the valve ball to lean against the partition plate, the liquid can smoothly flow, and when the liquid reversely enters the shell from the output pipe, the valve ball is extruded at one end close to the input pipe by the flowing of the liquid and the elasticity of the spring, so that the liquid is blocked and cannot flow; form the effect of the one-way circulation of fluid through two sets of separation mechanisms, when the buffer memory jar absorbs the culture solution, second separation mechanism can prevent that external air from being inhaled, and when buffer memory jar discharge culture solution, first separation mechanism can prevent that the culture solution from flowing back to in the culture dish to avoided the culture solution to receive external pollution's problem. The utility model has the advantages of simple integral structure, easy manufacture and realization, low cost, and can satisfy the demand of aseptic sampling of culture solution, convenient use.
Drawings
FIG. 1 is a schematic view of a cell culture solution sterile sampling device;
FIG. 2 is a schematic structural diagram of a first blocking mechanism in the cell culture solution sterile sampling device.
In the figure: 1-cache tank, 2-connecting pipe, 3-sampling needle, 4-first separation mechanism, 5-sealing cover, 6-hose, 7-liquid outlet pipe, 8-second separation mechanism, 9-piston, 10-screw, 11-observation window, 12-shell, 13-input pipe, 14-output pipe, 15-partition board, 16-through hole, 17-spring and 18-valve ball.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1-2, the present invention provides a technical solution: the utility model provides an aseptic sampling device of cell culture liquid, includes buffer memory jar 1, 1 bottom of buffer memory jar is connected with first separation mechanism 4, 4 bottoms of first separation mechanism are connected with connecting pipe 2, sampling needle 3 is installed to 2 bottoms of connecting pipe, 1 top of buffer memory jar is provided with closing cap 5, be provided with suction means on the closing cap 5, the one end of buffer memory jar 1 is connected with hose 6, the one end that buffer memory jar 1 was kept away from to hose 6 is connected with second separation mechanism 8, the one end that hose 6 was kept away from to second separation mechanism 8 is connected with drain pipe 7.
The lateral wall surface of buffer tank 1 is provided with transparent observation window 11, and observation window 11 is convenient for observe the liquid capacity in buffer tank 1.
The surface of the observation window 11 is provided with a graduated scale.
The suction mechanism comprises a screw rod 10, a threaded hole corresponding to the screw rod 10 is formed in the surface of the sealing cover 5, a knob is installed at the top end of the screw rod 10, the bottom end of the screw rod extends to the inside of the cache tank 1, and the bottom end of the screw rod is rotatably connected with a piston 9.
The first blocking mechanism 4 and the second blocking mechanism 8 are identical in structure and comprise a shell 12, an input pipe 13 is arranged at one end of the shell 12, an output pipe 14 is arranged at the other end of the shell, a partition plate 15 is arranged inside the shell 12, a plurality of through holes 16 are formed in the surface of the partition plate 15, a valve ball 18 is connected to one end, facing the input pipe 13, of the partition plate 15 through a spring 17, the diameter of the valve ball 18 is larger than the pipe diameter of the input pipe 13, when liquid is input from the input pipe 13, the pressure overcomes the elastic force of the spring 17 to enable the valve ball 18 to lean against the partition plate 15, the liquid can flow smoothly, when the liquid reversely enters the shell 12 from the output pipe 14, the valve ball 18 is extruded at one end close to the input pipe 13 by the flowing of the liquid and the elastic.
The utility model discloses a theory of operation: during the use, prick the sample needle in the culture dish, through rotatory screw rod 10, drive piston 9 and shift up, form the negative pressure in buffer memory jar 1, inhale buffer memory jar 1 with the culture solution in, the screw rod 10 of then counter-rotating makes piston 9 descend, utilizes pressure to crowd the culture solution in buffer memory jar 1 in hose 6, discharges from drain pipe 7 to the household utensils of waiting to shift.
It is obvious to a person skilled in the art that the invention is not restricted to details of the above-described exemplary embodiments, but that it can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (6)
1. The utility model provides an aseptic sampling device of cell culture liquid, includes buffer tank (1), its characterized in that: buffer memory jar (1) bottom is connected with first separation mechanism (4), first separation mechanism (4) bottom is connected with connecting pipe (2), sample needle (3) are installed to connecting pipe (2) bottom, buffer memory jar (1) top is provided with closing cap (5), be provided with suction mechanism on closing cap (5), the one end of buffer memory jar (1) is connected with hose (6), the one end that buffer memory jar (1) was kept away from in hose (6) is connected with second separation mechanism (8), the one end that hose (6) were kept away from in second separation mechanism (8) is connected with drain pipe (7).
2. The device according to claim 1, wherein: and a transparent observation window (11) is arranged on the surface of the side wall of the cache tank (1).
3. A device for sterile sampling of cell culture fluid according to claim 2, wherein: and a graduated scale is arranged on the surface of the observation window (11).
4. The device according to claim 1, wherein: the suction mechanism comprises a screw rod (10), a threaded hole corresponding to the screw rod (10) is formed in the surface of the sealing cover (5), a knob is installed at the top end of the screw rod (10), the bottom end of the screw rod extends to the interior of the buffer tank (1), and the bottom end of the screw rod is rotatably connected with a piston (9).
5. The device according to claim 1, wherein: first separation mechanism (4) and second separation mechanism (8) structure are the same, all include casing (12), the one end of casing (12) is provided with input tube (13), and the other end is provided with output tube (14), casing (12) inside is provided with baffle (15), a plurality of through-holes (16) have been seted up on baffle (15) surface, baffle (15) are connected with valve ball (18) through spring (17) towards the one end of input tube (13), the diameter of valve ball (18) is greater than the pipe diameter of input tube (13).
6. The device according to claim 5, wherein: input tube (13) of first separation mechanism (4) are connected with connecting pipe (2), and output tube (14) are connected with buffer tank (1), input tube (13) of second separation mechanism (8) are connected with hose (6), and output tube (14) are connected with drain pipe (7).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021813903.9U CN213357583U (en) | 2020-08-26 | 2020-08-26 | Aseptic sampling device of cell culture liquid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021813903.9U CN213357583U (en) | 2020-08-26 | 2020-08-26 | Aseptic sampling device of cell culture liquid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN213357583U true CN213357583U (en) | 2021-06-04 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202021813903.9U Active CN213357583U (en) | 2020-08-26 | 2020-08-26 | Aseptic sampling device of cell culture liquid |
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| Country | Link |
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| CN (1) | CN213357583U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113430104A (en) * | 2021-07-22 | 2021-09-24 | 镇江市建设工程质量检测中心有限公司 | Suspension spraying box |
| CN118240658A (en) * | 2024-03-26 | 2024-06-25 | 江苏育瑞康生物科技有限公司 | Automatic NK culture medium adding device for activating PBMC (human immunodeficiency Virus) cells |
-
2020
- 2020-08-26 CN CN202021813903.9U patent/CN213357583U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113430104A (en) * | 2021-07-22 | 2021-09-24 | 镇江市建设工程质量检测中心有限公司 | Suspension spraying box |
| CN118240658A (en) * | 2024-03-26 | 2024-06-25 | 江苏育瑞康生物科技有限公司 | Automatic NK culture medium adding device for activating PBMC (human immunodeficiency Virus) cells |
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