CN107460123B - Improved femoral head H subtype vascular endothelial cell culture device - Google Patents

Improved femoral head H subtype vascular endothelial cell culture device Download PDF

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CN107460123B
CN107460123B CN201710653782.2A CN201710653782A CN107460123B CN 107460123 B CN107460123 B CN 107460123B CN 201710653782 A CN201710653782 A CN 201710653782A CN 107460123 B CN107460123 B CN 107460123B
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nutrient solution
cell culture
culture device
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solution injection
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CN107460123A (en
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孙伟
高福强
李子荣
张庆宇
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China Japan Friendship Hospital
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China Japan Friendship Hospital
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/22—Transparent or translucent parts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters

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Abstract

The invention discloses an improved femoral head H subtype vascular endothelial cell culture device, which comprises a shell, wherein the lower end of the shell is provided with a culture dish inlet, the upper end of the shell is rotationally connected with a transparent sleeve joint shell, the middle part of the transparent sleeve joint shell is slidably connected with a nutrient solution injection pipe, the lower end of the nutrient solution injection pipe is integrally formed with a nutrient solution outlet, the bottom surface of the culture dish inlet is fixedly connected with a screw rod, the outer side wall of the screw rod is in threaded connection with an extrusion sleeve, and the upper end of the extrusion sleeve is attached with a culture device.

Description

Improved femoral head H subtype vascular endothelial cell culture device
Technical Field
The invention relates to the technical field of cell culture devices, in particular to an improved femoral head H subtype vascular endothelial cell culture device.
Background
The cell culture device is often used when culturing cells, and the culture dish is often used for culturing cells in a sterile space, but the sterile space cannot be completely sealed with the outside, so that the completely sterile effect cannot be realized, and the culture dish is manually added with culture solution one by one when culturing the cells, so that the working time of people is prolonged, and an improved femoral head H subtype vascular endothelial cell culture device is provided.
Disclosure of Invention
The invention aims to provide an improved femoral head H subtype vascular endothelial cell culture device, which solves the problems in the background technology.
In order to achieve the above purpose, the present invention provides the following technical solutions: the utility model provides an improved femoral head H subtype vascular endothelial cell culture device, includes the casing, the culture dish is offered to the lower extreme of casing and is put into the mouth, the middle part left end fixedly connected with active gas inlet port of lateral wall of casing, the right-hand member fixedly connected with exhaust gas discharge port of lateral wall of casing, the upper end of casing rotates and is connected with transparent cover shell, the front end of the upper surface of transparent cover shell is embedded with the observation hole that enlarges, and is equipped with biological cell sampling hole on the right side of the observation hole that enlarges, the middle part sliding connection of transparent cover shell has the nutrient solution injection tube, and nutrient solution injection tube and transparent cover shell junction are equipped with first spring, the upper end integrated into one piece of nutrient solution injection tube has the screw connection mouth, the lower extreme integrated into one piece of nutrient solution injection tube has the nutrient solution export, the inner chamber lower extreme of nutrient solution injection tube has cup jointed the shutoff stopper, the upper surface laminating of shutoff has the second spring, the bottom surface fixedly connected with screw rod of culture dish put into the mouth, the lateral wall spiro union of screw rod has the extrusion cover, the outer side wall integrated into one piece has the carousel, the upper end laminating of carousel;
the culture device comprises a supporting plate, the bottom surface of the supporting plate is attached to the extrusion sleeve, a cell culture groove is formed in the upper surface of the supporting plate in an integrated mode, a limit ring is fixedly connected to the middle of the upper surface of the supporting plate, a nutrient solution outflow hole is formed in the outer side wall of the limit ring, the nutrient solution outflow hole is communicated with the cell culture groove, a jacking block is fixedly connected to the middle of the limit ring, and the jacking block is opposite to the plugging plug.
Preferably, the upper surface of the supporting plate is attached with a rubber ring.
Preferably, the upper surface of the extrusion sleeve is rotatably connected with a rotating ring.
Compared with the prior art, the invention has the beneficial effects that: the invention discloses an improved femoral head H subtype vascular endothelial cell culture device, which is characterized in that a shell, a transparent sleeve joint shell and a supporting plate are used, so that a cell culture tank is completely placed in a closed space, external bacteria are prevented from entering, and meanwhile, the function of adding culture solutions together is realized through the matched use of a nutrient solution injection pipe and a top block, and the problem of time waste caused by adding the culture solutions one by one is avoided.
Drawings
FIG. 1 is a schematic diagram of the structure of the present invention;
FIG. 2 is a cross-sectional view of the structure of the present invention;
FIG. 3 is a structural view of a culture apparatus according to the present invention;
fig. 4 is a schematic diagram of the structure of the plugging plug of the invention.
In the figure: 1 a shell, 2 a culture dish inlet, 3 an active gas inlet, 4 an exhaust gas outlet, 5 a transparent sleeve joint shell, 6 an amplifying observation hole, 7 a biological cell sampling hole, 8 a nutrient solution injection pipe, 9 a first spring, 10 a screw joint, 11 a nutrient solution outlet, 12 a sealing plug, 13 a second spring, 14 a screw rod, 15 a squeezing sleeve, 16 a turntable, 17 a culture device, 171 a supporting plate, 172 a cell culture tank, 173 a limiting ring, 174 a nutrient solution outflow hole, 175 a top block, 18 a rubber ring, 19 a rotating ring and 20 a sealing cover.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to fig. 1-4, the present invention provides a technical solution: the utility model provides an improved femoral head H subtype vascular endothelial cell culture device, includes casing 1, culture dish is put into mouth 2 has been seted up to casing 1's lower extreme, the middle part left end fixedly connected with active gas inlet port 3 of casing 1's lateral wall, the right-hand member fixedly connected with exhaust gas outlet port 4 of casing 1's lateral wall, casing 1's upper end is rotated and is connected with transparent cover shell 5, the front end of transparent cover shell 5's upper surface is embedded with and is enlargies viewing aperture 6, and the right side of enlargies viewing aperture 6 is equipped with biological cell sampling hole 7, the middle part sliding connection of transparent cover shell 5 has nutrient solution injection tube 8, and nutrient solution injection tube 8 and transparent cover shell 5 junction is equipped with first spring 9, the upper end integrated into one piece of nutrient solution injection tube 8 has screw connection 10, the lower extreme integrated into one piece of nutrient solution injection tube 8 has nutrient solution export 11, the inner chamber lower extreme of nutrient solution injection tube 8 has the cover to connect shutoff plug 12, the upper surface laminating of shutoff plug 12 has second spring 13, culture dish bottom surface is put into mouth 2's middle part sliding connection has nutrient solution injection tube 8, the screw connection 15 has the screw rod 15 extrusion sleeve 15 to extrude the lateral wall 15, extrusion device has the outer side 15 of extrusion sleeve 15;
the culture device 17 comprises a support plate 171, the bottom surface of the support plate 171 is attached to the extrusion sleeve 15, a cell culture groove 172 is uniformly formed on the upper surface of the support plate 171 in an integrated manner, a limit ring 173 is fixedly connected to the middle of the upper surface of the support plate 171, a nutrient solution outflow hole 174 is formed in the outer side wall of the limit ring 173, the nutrient solution outflow hole 174 is communicated with the cell culture groove 172, a top block 175 is fixedly connected to the middle of the limit ring 173, and the top block 175 is opposite to the plugging plug 12.
The invention realizes that the cell culture tank is completely placed in the closed space through the use of the shell 1, the transparent sleeve-joint shell 5 and the supporting plate 171, thereby avoiding the entry of external bacteria, and simultaneously realizes the function of adding culture solution together through the matched use of the nutrient solution injection pipe 8 and the top block 175, thereby avoiding the problem of time waste caused by adding the culture solution one by one.
Specifically, the rubber ring 18 is attached to the upper surface of the support plate 171, so that the support plate 171 is prevented from being connected with the bottom surface of the housing 1 in a loose manner, and bacteria can enter.
Specifically, a rotating ring 19 is rotatably connected to the upper surface of the pressing sleeve 15, so as to prevent the support plate 171 from rotating when the pressing sleeve 15 is rotated.
Specifically, the upper end of the biological cell sampling hole 7 is screwed with a plugging cover 20 to prevent external bacteria from entering the housing 1 from the biological cell sampling hole 7.
Working principle: when the invention is used, cultured cells are placed into the cell culture tank 172, then the supporting plate 171 is used for preventing the cell culture tank 172 from being extruded into the shell 1 by the extrusion sleeve 15, meanwhile, the bottle of external nutrient solution is screwed on the screw connector 10, when people need to add the nutrient solution into the cell culture tank 172 and press the screw connector 10, the jacking block 175 can extrude the plugging plug 12 at the moment, the nutrient solution can flow into the limit ring 173 from the nutrient solution injection pipe 8, so that the nutrient solution can flow into the cell culture tank 172 from the nutrient solution outflow hole 174 on the limit ring 173, meanwhile, active gas can be introduced from the culture dish inlet 2, and waste gas can be discharged from the waste gas outlet 4, and when people need to carefully observe the cultured cells in the cell culture tank 172, the transparent sleeve shell 5 can be rotated and the amplified observation hole 6 can be observed.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (3)

1. An improved femoral head H subtype vascular endothelial cell culture device comprises a shell (1), and is characterized in that: the culture dish is put into mouth (2) has been seted up to the lower extreme of casing (1), the middle part left end fixedly connected with active gas inlet port (3) of the lateral wall of casing (1), the right-hand member fixedly connected with waste gas discharge port (4) of the lateral wall of casing (1), the upper end rotation of casing (1) is connected with transparent cover shell (5), the front end embedding of the upper surface of transparent cover shell (5) has enlarged observation hole (6), and the right side of enlarged observation hole (6) is equipped with biological cell sample hole (7), the middle part sliding connection of transparent cover shell (5) has nutrient solution injection tube (8), and nutrient solution injection tube (8) are equipped with first spring (9) with transparent cover shell (5) junction, the upper end integrated into one piece of nutrient solution injection tube (8) has screw connection (10), the lower extreme integrated into one piece of nutrient solution injection tube (8) has nutrient solution export (11), the inner chamber lower extreme cover of nutrient solution injection tube (8) has cup-in contact plug (12), the upper surface laminating of plug (12) is equipped with biological cell sample hole (7), the shutoff top surface (16) has extrusion screw connection (14) of the outer side of screw connection (14), a culture device (17) is attached to the upper end of the extrusion sleeve (15); culture apparatus (17) are including backup pad (171), the bottom surface and the extrusion cover (15) of backup pad (171) laminate mutually, the even integrated into one piece of upper surface of backup pad (171) has cell culture groove (172), the middle part fixedly connected with spacing collar (173) of the upper surface of backup pad (171), nutrient solution outflow hole (174) have been seted up to the lateral wall of spacing collar (173), nutrient solution outflow hole (174) communicate with each other with cell culture groove (172), the middle part fixedly connected with kicking block (175) of spacing collar (173), kicking block (175) are relative with shutoff stopper (12), the upper end spiro union of biological cell sampling hole (7) has shutoff lid (20).
2. An improved vascular endothelial cell culture device of the femoral head subtype H as claimed in claim 1, wherein: the upper surface of the supporting plate (171) is attached with a rubber ring (18).
3. An improved vascular endothelial cell culture device of the femoral head subtype H as claimed in claim 1, wherein: the upper surface of the extrusion sleeve (15) is rotatably connected with a rotating ring (19).
CN201710653782.2A 2017-07-19 2017-07-19 Improved femoral head H subtype vascular endothelial cell culture device Active CN107460123B (en)

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CN110331095A (en) * 2019-06-11 2019-10-15 华夏源（上海）细胞基因工程股份有限公司 A kind of stem cell nutrient solution adding device and its application method
CN112997949B (en) * 2021-03-03 2023-05-05 赵福振 Fish nutrient solution culture device rich in bioactive nano organic zinc selenium
CN112899137B (en) * 2021-04-09 2022-10-21 赵福振 Fermentation device for pickled products rich in organic biological zinc and selenium

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CN205528839U (en) * 2016-04-22 2016-08-31 广东普奥思生物科技有限公司 Novel cell culture device
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CN201817469U (en) * 2010-09-15 2011-05-04 薛梅 Automatic timing and time-counting liquid changing device for cell culture
JP2014128247A (en) * 2012-12-28 2014-07-10 National Cancer Center Cell cultivation container, cell cultivation tool, and cell cultivation method
CN204918612U (en) * 2015-09-07 2015-12-30 上海逍鹏生物科技有限公司 High density cell culture bucket
CN205528839U (en) * 2016-04-22 2016-08-31 广东普奥思生物科技有限公司 Novel cell culture device
CN205590714U (en) * 2016-04-28 2016-09-21 天津卫凯生物工程有限公司 A gaseous feeding device for cell perfusion culture
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