CN110331095A - A kind of stem cell nutrient solution adding device and its application method - Google Patents
A kind of stem cell nutrient solution adding device and its application method Download PDFInfo
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- CN110331095A CN110331095A CN201910499312.4A CN201910499312A CN110331095A CN 110331095 A CN110331095 A CN 110331095A CN 201910499312 A CN201910499312 A CN 201910499312A CN 110331095 A CN110331095 A CN 110331095A
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- nutrient solution
- culture dish
- stem cell
- adding device
- fixed plate
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
Abstract
The invention discloses a kind of stem cell nutrient solution adding device and its application methods, belong to cell culture apparatus field, a kind of stem cell nutrient solution adding device, including culture dish ontology and culture dish lid, digging from bottom to top in the middle part of culture dish lid has cylinder shape groove, and movable plate is equipped on the inside of cylindrical type groove, movable plate lower end is fixedly connected with seal boss, fixed plate is equipped on the downside of seal boss, fixed plate side is fixedly connected with cylinder shape groove inner wall, digging in fixed plate has multiple first leak holes, corresponding dig has the second leak hole on movable plate and seal boss, it may be implemented to exempt to uncap that nutrient solution can be added, the problem of avoiding germ contamination during taking off lid addition nutrient solution, and the device can guarantee the uniform addition of nutrient solution, so that the nutrient solution surface being added tends to be concordant, to guarantee that culture medium is equal to the full extent Even contact nutrient solution, and then promote the quality of culture medium.
Description
Technical field
The present invention relates to cell culture apparatus field, more specifically to a kind of stem cell nutrient solution adding device and
Its application method.
Background technique
Stem cell is a kind of multipotential cell with the of self-replication capacity.Under certain condition, it can be divided into more
Kind functioning cell.The stage of development according to locating for stem cell is divided into embryonic stem cell and adult stem cell.According to the hair of stem cell
It educates potential to be divided into three classes: myeloid-lymphoid stem cell, multipotential stem cell and unipotent stem cell.Stem cell is a kind of inabundant differentiation, still not
Mature cell, has the potential function for regenerating various histoorgans and human body, and medical field is known as " general-purpose cell ".
And candidate stem cell is the adult stem cell in hematological system, is a heterogeneous group, have it is long-term self
The ability of update and the potential for being divided into all kinds of mature blood cells.
Candidate stem cell cannot survive for a long time in simple basal medium, must provide in cell culture
The nutrient solution and growth factor of certain constant basis just can enable cell grow and maintain growth conditions, therefore the basis used
Culture medium is often required to addition serum, and serum is made of the different biomolecule of many sizes, it is capable of providing cell and exists
Required growth factor, hormone and binding protein when in vitro culture, therefore need to control battalion in candidate stem cell incubation
Support the amount of substance.
Existing stem cell nutrient solution addition manner is usually to add the cover lifting of culture dish, is caused in adding procedure
The culture medium inside culture medium is polluted in the entrance of extraneous bacterium；There are also nutrient solution injection is directly provided on culture dish lid
Osculum, but the nutrient solution injected is easy local accumulation in media surface, leads to the nutrient solution uneven surface being added, culture is not
, culture medium quality is reduced.
Summary of the invention
1. technical problems to be solved
Aiming at the problems existing in the prior art, the purpose of the present invention is to provide a kind of stem cell nutrient solution adding devices
And its application method, it may be implemented to exempt to uncap that nutrient solution can be added, and it is dirty to avoid bacterium during taking off lid addition nutrient solution
The problem of dye, and the device can guarantee the uniform addition of nutrient solution, so that the nutrient solution surface being added tends to concordantly, thus most
Guarantee that culture medium uniformly contacts nutrient solution in big degree, and then promotes the quality of culture medium.
2. technical solution
To solve the above problems, the present invention adopts the following technical scheme that.
A kind of stem cell nutrient solution adding device, including culture dish ontology and culture dish lid, the culture dish cover cap
In culture dish ontology upper end, culture dish ontology upper end, which is dug, annular seal groove, and culture dish lid lower end is fixed to be connected
It is connected to the sealing ring to match with annular seal groove, digging from bottom to top in the middle part of the culture dish lid has cylinder shape groove, and
Movable plate is equipped on the inside of cylindrical type groove, the cylinder shape groove inner wall cutting has the annular turn trough to match with movable plate, and
Movable plate side is slidably connected with annular turn trough, and the movable plate lower end is fixedly connected with seal boss, under the seal boss
Side is equipped with fixed plate, and the fixed plate side is fixedly connected with cylinder shape groove inner wall, and cutting has multiple the in the fixed plate
One leak hole, corresponding dig has the second leak hole on the movable plate and seal boss, one a pair of second leak hole and the first leak hole
It answers, L-type rocking bar is fixedly connected at the dead plate upper end center, culture dish lid upper end, which is dug, through-hole, the L-type
The one end of rocking bar far from fixed plate extends on the upside of culture dish lid through through-hole, and culture dish lid upper end, which is dug, charging
Hole is fixedly connected with fixed feed pipe at the feed opening, and fixed feed pipe upper end is plugged with sealing-plug, may be implemented to exempt to uncap
The problem of nutrient solution can be added, avoid germ contamination during taking off lid addition nutrient solution, and the device can guarantee nutrition
The uniform addition of liquid, so that the nutrient solution surface being added tends to concordantly, to guarantee that culture medium uniformly contacts battalion to the full extent
Nutrient solution, and then promote the quality of culture medium.
Further, there is connection sealing bearing between the through-hole wall and L-type rocking bar, and seal Selection of Bearings nachi
Bearing, seal bearing is arranged the leakproofness guaranteed inside cylinder shape groove while can guarantee smooth rotation L-type rocking bar.
Further, the fixed feed pipe lower end through feed opening and extends on the inside of cylinder shape groove, nutrient solution by
Fixed feed pipe imports inside cylinder shape groove, and lower end avoids can guarantee battalion with the setting of cylinder shape groove inner top flush
Nutrient solution is without being adhered to cylinder shape groove inner top.
Further, the described one end of L-type rocking bar far from fixed plate is dug from top to bottom threaded hole, at the threaded hole
It is threaded with lifting screw, is screwed down lifting screw manually, so that lower end of the lifting screw is tight against culture dish top surface, it can
L-type rocking bar is fixed, so that L-type rocking bar is not easy accidental displacement, the culture solution inside cylinder shape groove is not easily caused to accidentally enter
In culture dish ontology.
Further, the lower end of the lifting screw is fixedly connected with sucker, and fixing sleeve is connected to suction in the middle part of the lifting screw
Ring, and ring is inhaled between L-type rocking bar and sucker, the sucker stabilization of lower end of the lifting screw is adsorbed on culture dish top surface, increases
The fixed effect of strong L-type rocking bar；When needing to rotate L-type rocking bar, can manual-up rotation lifting screw so that inhale ring upper end stablize
L-type rocker surfaces are adsorbed in, accordingly even when stripped thread phenomenon occurs in lifting screw, under the suction-operated for inhaling ring, lifting screw is not
The easy rotation glided contact culture dish top surface and hinder L-type rocking bar.
Further, the cylindrical type groove inner wall, the first leak hole and the second leak hole inner wall are coated with composite type modified
Anti-stick coating, the coating of the anti-stick coating include nanometer non-viscous paint JC316, and JC316 substantially reduces substrate wall surface surface energy,
It can effectively prevent the attachment of nutrient solution is counter from gluing, guarantee that nutrient solution is smoothly added in culture dish ontology culture dish ontology.
Further, culture dish lid side, which is longitudinally carved, is equipped with scale line, and the scale line is equipped with a pair, and a pair of
Scale line is symmetrically distributed in culture dish lid side, operator when observing the liquid level of nutrient solution, symmetrical scale line
The personnel's accurate reading that is convenient to operation is set, and operator only needs to keep its sight with corresponding a pair of of scale line in same water
On horizontal line, it can reduce and misread several probability, improve the addition accuracy of nutrient solution.
Further, the width of the annular seal groove is greater than the thickness of sealing ring, is placed in the annular seal groove
Water, and the liquid level of water is lower than the notch of annular seal groove, the gap filling water vacated between annular seal groove and sealing ring utilizes
The mobility of water plays good sealing function, guarantees the good leakproofness of culture dish body interior.
Further, the inner sidewall of the annular seal groove is higher than the lateral wall of annular seal groove, avoids annular seal groove
In water cover annular seal groove and enter in culture dish ontology, pollute culture medium.
A kind of stem cell nutrient solution adding device, application method are as follows:
S1, sealing-plug is opened, adds nutrient solution into cylinder shape groove from fixed feed pipe；
S2, Bian Tianjia nutrient solution side level observe scale line, when the liquid level of nutrient solution reaches aequum, stop addition,
It is stoppered sealing-plug；
S3, unscrew lifting screw, so that sucker rotates L-type rocking bar, so that fixed plate far from culture dish top surface manually
On the first leak hole be slowly overlapped with the second leak hole on movable plate, the nutrient solution in cylinder shape groove from the gap of coincidence
In even instillation culture dish ontology；
S4, after being added dropwise, L-type rocking bar is rotated backward, so that the first leak hole in fixed plate and second on movable plate
Leak hole is staggered, and guarantees the leakproofness of culture dish body interior.
3. beneficial effect
Compared with the prior art, the present invention has the advantages that
(1) this programme may be implemented to exempt to uncap that nutrient solution can be added, and avoid bacterium during taking off lid addition nutrient solution
The problem of pollution, and the device can guarantee the uniform addition of nutrient solution, so that the nutrient solution surface being added tends to concordantly, thus
Guarantee that culture medium uniformly contacts nutrient solution to the full extent, and then promotes the quality of culture medium.
(2) there is connection sealing bearing between through-hole wall and L-type rocking bar, and seal Selection of Bearings nachi bearing, seal axis
The setting held guarantees the leakproofness inside cylinder shape groove while can guarantee smooth rotation L-type rocking bar.
(3) fixed feed pipe lower end through feed opening and extends on the inside of cylinder shape groove, and nutrient solution is by fixing feed pipe
It imports inside cylinder shape groove, lower end avoids can guarantee that nutrient solution will not be attached with the setting of cylinder shape groove inner top flush
In cylinder shape groove inner top.
(4) one end of L-type rocking bar far from fixed plate is dug from top to bottom threaded hole, and lifting is threaded at threaded hole
Screw rod is screwed down lifting screw manually, so that lower end of the lifting screw can fix L-type rocking bar tight against culture dish top surface,
So that L-type rocking bar is not easy accidental displacement, the culture solution inside cylinder shape groove is not easily caused to accidentally enter in culture dish ontology.
(5) lower end of the lifting screw is fixedly connected with sucker, and fixing sleeve is connected to suction ring in the middle part of lifting screw, and inhales ring and be located at L
Between type rocking bar and sucker, the sucker of lower end of the lifting screw, which is stablized, is adsorbed on culture dish top surface, enhances the fixation of L-type rocking bar
Effect；When needing to rotate L-type rocking bar, can manual-up rotation lifting screw so that inhale ring upper end stabilization be adsorbed in L-type rocking bar table
Face, accordingly even when stripped thread phenomenon occurs in lifting screw, under the suction-operated for inhaling ring, lifting screw is not easy the contact culture dish that glides
Top surface and the rotation for hindering L-type rocking bar.
(6) cylindrical type groove inner wall, the first leak hole and the second leak hole inner wall are coated with composite type modified anti-stick coating, prevent
The coating of adhesive coating layer includes nanometer non-viscous paint JC316, and JC316 substantially reduces substrate wall surface surface energy, can effectively prevent
The attachment of nutrient solution is counter to glue, and guarantees that nutrient solution is smoothly added in culture dish ontology culture dish ontology.
(7) culture dish lid side, which is longitudinally carved, is equipped with scale line, and scale line is equipped with a pair, and a pair of of scale line is symmetrical
In culture dish lid side, when observing the liquid level of nutrient solution, the setting of symmetrical scale line is convenient to operation operator
Personnel's accurate reading, operator only need to keep its sight and corresponding a pair of of scale line in the same horizontal line, can
Several probability is misread in reduction, improves the addition accuracy of nutrient solution.
(8) width of annular seal groove is greater than the thickness of sealing ring, is placed with water in annular seal groove, and the liquid level of water is low
In the notch of annular seal groove, the gap filling water vacated between annular seal groove and sealing ring is played using the mobility of water
Good sealing function guarantees the good leakproofness of culture dish body interior.
(9) inner sidewall of annular seal groove is higher than the lateral wall of annular seal groove, and the water in annular seal groove is avoided to cover
Annular seal groove enters in culture dish ontology, pollutes culture medium.
Detailed description of the invention
Fig. 1 is the perspective view of the invention covered under culture dish lid state；
Fig. 2 is the structural schematic diagram in Fig. 1 at A；
Fig. 3 is the schematic perspective view under present invention opening culture dish lid state；
Fig. 4 is culture dish lid transverse direction fractionation structural representation of the invention；
Fig. 5 is the structural schematic diagram that the leak hole on movable plate and fixed plate of the invention coincides under state；
Fig. 6 is the structural schematic diagram that the leak hole on movable plate and fixed plate of the invention is staggered under open state；
Fig. 7 is that the culture dish lid transverse direction fractionation structure that the present invention is locally added for the progress of special constant temperature nutrient solution is shown
It is intended to；
Culture dish lid when Fig. 8 is progress part addition of the present invention for special constant temperature nutrient solution laterally splits structure
Schematic diagram.
Figure label explanation:
1 culture dish ontology, 2 culture dish lids, 3 sealing rings, 4 annular seal grooves, 5L type rocking bar, 6 sealing-plugs, 7 it is fixed into
Ring, 10 suckers, 11 fixed plates, 12 movable plates, 13 seal bosses, 14 first leak holes, 15 second leakages are inhaled in expects pipe, 8 lifting screws, 9
Hole, 16 sealing bearings, 17 movable feed pipes, 18 charging apertures.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description；Obviously, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments, is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that term " on ", "lower", "inner",
The orientation or positional relationship of the instructions such as "outside", " top/bottom end " is to be based on the orientation or positional relationship shown in the drawings, only
It is that for the convenience of describing the present invention and simplifying the description, rather than the device or element of indication or suggestion meaning must have specifically
Orientation is constructed and operated in a specific orientation, therefore is not considered as limiting the invention.In addition, term " first ", " the
Two " are used for descriptive purposes only and cannot be understood as indicating or suggesting relative importance.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " installation " " is set
Be equipped with ", " be arranged/connect ", " connection " etc., shall be understood in a broad sense, such as " connection ", may be a fixed connection, be also possible to removable
Connection is unloaded, or is integrally connected；It can be mechanical connection, be also possible to be electrically connected；It can be directly connected, it can also be in
Between medium be indirectly connected, can be the connection inside two elements.It for the ordinary skill in the art, can be specific
Situation understands the concrete meaning of above-mentioned term in the present invention.
Embodiment 1:
Fig. 1 and Fig. 2, a kind of stem cell nutrient solution adding device, including culture dish ontology 1 and culture dish lid 2 are please referred to,
Culture dish lid 2 is placed on 1 upper end of culture dish ontology, please refers to Fig. 3 and Fig. 4,1 upper end of culture dish ontology, which is dug, annular seal groove
4,2 lower end of culture dish lid is fixedly connected with the sealing ring 3 to match with annular seal groove 4,2 middle part of culture dish lid from lower and
Upper cutting has cylinder shape groove, and movable plate 12 is equipped on the inside of cylindrical type groove, and the cutting of cylinder shape groove inner wall has and movable plate
12 annular turn troughs to match, and 12 side of movable plate is slidably connected with annular turn trough, please refer to Fig. 5 and Fig. 6, under movable plate 12
End is fixedly connected with seal boss 13, is equipped with fixed plate 11 on the downside of seal boss 13, in 11 side of fixed plate and cylinder shape groove
Wall is fixedly connected, and digging in fixed plate 11 has multiple first leak holes 14, and corresponding dig has the on movable plate 12 and seal boss 13
Two leak holes 15, the second leak hole 15 and the first leak hole 14 correspond, and are fixedly connected with L-type rocking bar 5 at 11 upper end center of fixed plate,
2 upper end of culture dish lid, which is dug, through-hole, and the one end of L-type rocking bar 5 far from fixed plate 11 extends to culture dish lid 2 through through-hole
Upside, 2 upper end of culture dish lid, which is dug, feed opening, and fixed feed pipe 7, and fixed feed pipe 7 are fixedly connected at feed opening
Upper end is plugged with sealing-plug 6, may be implemented to exempt to uncap that nutrient solution can be added, and it is dirty to avoid bacterium during taking off lid addition nutrient solution
The problem of dye, and the device can guarantee the uniform addition of nutrient solution, so that the nutrient solution surface being added tends to concordantly, thus most
Guarantee that culture medium uniformly contacts nutrient solution in big degree, and then promotes the quality of culture medium.
There is connection sealing bearing 16 between through-hole wall and L-type rocking bar 5, and seals bearing 16 and select nachi bearing, sealing
The setting of bearing 16 guarantees the leakproofness inside cylinder shape groove while can guarantee smooth rotation L-type rocking bar 5.
Fixed 7 lower end of feed pipe is through feed opening and extends on the inside of cylinder shape groove, and nutrient solution is led by fixing feed pipe 7
Enter inside cylinder shape groove, lower end avoids can guarantee that nutrient solution will not adhere to the setting of cylinder shape groove inner top flush
In cylinder shape groove inner top.
Fig. 7 and Fig. 8 are please referred to, 2 upper end of culture dish lid, which is also dug, another feed opening, is slidably connected at the feed opening
There is movable feed pipe 17, and 17 upper end of movable feed pipe is equally plugged with sealing-plug 6, fixed plate 11, movable plate 12 and seal boss
13 corresponding dig have charging aperture 18, which is not overlapped simultaneously with the first leak hole 14 and the second leak hole 15, i.e., fixed plate 11,
When charging aperture 18 on movable plate 12 and seal boss 13 overlaps, on fixed plate 11, movable plate 12 and seal boss 13
First leak hole 14 and the second leak hole 15 mutually stagger, at this time can be by movable feed pipe 17 to underthrust until under movable feed pipe 17
End extends in culture dish ontology 1, adds hot liquid into cylinder shape groove using fixed feed pipe 7, then feeds from activity
Pipe 17 directly adds nutrient solution into culture dish ontology 1, it is ensured that special constant temperature nutrient solution guarantees it during addition
Temperature-resistant, this kind of addition manner is suitable for the carry out part addition of special constant temperature nutrient solution.
The one end of L-type rocking bar 5 far from fixed plate 11 is dug from top to bottom threaded hole, and lifting is threaded at threaded hole
Screw rod 8 is screwed down lifting screw 8 manually, so that 8 lower end of lifting screw is tight against 2 surface of culture dish lid, it can be by L-type rocking bar 5
It is fixed, so that L-type rocking bar 5 is not easy accidental displacement, the culture solution inside cylinder shape groove is not easily caused to accidentally enter culture dish sheet
In body 1.
8 lower end of lifting screw is fixedly connected with sucker 10, and 8 middle part fixing sleeve of lifting screw, which is connected to, inhales ring 9, and inhales 9, ring
Between L-type rocking bar 5 and sucker 10, the sucker 10 of 8 lower end of lifting screw, which is stablized, is adsorbed on 2 surface of culture dish lid, enhances L-type
The fixed effect of rocking bar 5；When needing to rotate L-type rocking bar 5, can manual-up rotation lifting screw 8 so that inhale 9 upper end of ring stablize
It is adsorbed in 5 surface of L-type rocking bar, accordingly even when there is stripped thread phenomenon in lifting screw 8, under the suction-operated for inhaling ring 9, lifting screw
8 are not easy the rotation that downslide contacts 2 surface of culture dish lid and hinders L-type rocking bar 5.
Cylindrical type groove inner wall, the first leak hole 14 and 15 inner wall of the second leak hole are coated with composite type modified anti-stick coating,
The coating of anti-stick coating includes nanometer non-viscous paint JC316, and JC316 substantially reduces substrate wall surface surface energy, can effectively hinder
Only the attachment of nutrient solution is counter glues, and guarantees that nutrient solution is smoothly added in culture dish ontology culture dish ontology 1.
2 side of culture dish lid, which is longitudinally carved, is equipped with scale line, and scale line is equipped with a pair, and a pair of of scale line is symmetrically distributed in
2 side of culture dish lid, when observing the liquid level of nutrient solution, the setting of symmetrical scale line is convenient to operation people operator
Member's accurate reading, operator only need to keep its sight and corresponding a pair of scale line can drop in the same horizontal line
It is low to misread several probability, improve the addition accuracy of nutrient solution.
The width of annular seal groove 4 is greater than the thickness of sealing ring 3, is placed with water in annular seal groove 4, and the liquid level of water is low
The gap filling water vacated between the notch of annular seal groove 4, annular seal groove 4 and sealing ring 3, is risen using the mobility of water
To good sealing function, guarantee the 1 good leakproofness in inside of culture dish ontology.
The inner sidewall of annular seal groove 4 is higher than the lateral wall of annular seal groove 4, and the water in annular seal groove 4 is avoided to cover
Annular seal groove 4 enters in culture dish ontology 1, pollutes culture medium.
A kind of stem cell nutrient solution adding device, application method are as follows:
S1, sealing-plug 6 is opened, adds nutrient solution into cylinder shape groove from fixed feed pipe 7；
S2, Bian Tianjia nutrient solution side level observe scale line, when the liquid level of nutrient solution reaches aequum, stop addition,
It is stoppered sealing-plug 6；
S3, unscrew lifting screw 8, so that sucker 10 rotates L-type rocking bar 5 manually, please join far from 2 surface of culture dish lid
Fig. 5 is read, so that the first leak hole 14 in fixed plate 11 is slowly overlapped with the second leak hole 15 on movable plate 12, in cylinder shape groove
Nutrient solution uniformly instilled in culture dish ontology 1 from the gap of coincidence；
S4, after being added dropwise, rotate backward L-type rocking bar 5, referring to Fig. 6, make the first leak hole 14 in fixed plate 11 with
The second leak hole 15 on movable plate 12 is staggered, and guarantees the leakproofness inside culture dish ontology 1.
Stem cell nutrient solution addition manner in compared to the prior art is easy to cause the entrance of extraneous bacterium, pollution culture
The problem of culture medium inside base, stem cell nutrient solution adding device in this programme can be with by the fixation feed pipe 7 of setting
The problem of realization, which exempts to uncap, can add nutrient solution, avoid germ contamination during taking off lid addition nutrient solution；In addition, compared to
The nutrient solution uneven surface added in the prior art, the drawbacks of reducing culture medium quality, the stem cell nutrient solution in this programme adds
Feeder apparatus has the movable plate 12 of the second leak hole 15 to guarantee nutrition by digging the fixed plate 11 for having the first leak hole 14 and corresponding cutting
The uniform addition of liquid, so that the nutrient solution surface being added tends to concordantly, to guarantee that culture medium uniformly contacts battalion to the full extent
Nutrient solution, and then promote the quality of culture medium.
The foregoing is intended to be a preferred embodiment of the present invention；But scope of protection of the present invention is not limited thereto.
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
It improves design and is subject to equivalent substitution or change, should be covered by the scope of protection of the present invention.
Claims (10)
1. a kind of stem cell nutrient solution adding device, including culture dish ontology (1) and culture dish lid (2), the culture ware lid
Body (2) is placed on culture dish ontology (1) upper end, it is characterised in that: culture dish ontology (1) upper end, which is dug, annular seal groove
(4), culture dish lid (2) lower end is fixedly connected with the sealing ring (3) to match with annular seal groove (4), the culture
Digging from bottom to top in the middle part of ware lid body (2) has cylinder shape groove, and is equipped with movable plate (12) on the inside of cylindrical type groove, the cylinder
Connected in star inner wall, which is dug, the annular turn trough to match with movable plate (12), and movable plate (12) side and annular turn trough sliding connect
It connects, movable plate (12) lower end is fixedly connected with seal boss (13), and fixed plate is equipped on the downside of the seal boss (13)
(11), fixed plate (11) side is fixedly connected with cylinder shape groove inner wall, and digging on the fixed plate (11) has multiple the
One leak hole (14), corresponding dig has the second leak hole (15) on the movable plate (12) and seal boss (13), second leak hole
(15) it corresponds with the first leak hole (14), is fixedly connected with L-type rocking bar (5) at fixed plate (11) upper end center, it is described
Culture dish lid (2) upper end, which is dug, through-hole, and the L-type rocking bar (5) extends to far from the one end of fixed plate (11) through through-hole
On the upside of culture dish lid (2), culture dish lid (2) upper end, which is dug, feed opening, is fixedly connected at the feed opening
Determine feed pipe (7), and fixed feed pipe (7) upper end is plugged with sealing-plug (6).
2. a kind of stem cell nutrient solution adding device according to claim 1, it is characterised in that: the through-hole wall and L
There is connection sealing bearing (16) between type rocking bar (5), and seals bearing (16) and select nachi bearing.
3. a kind of stem cell nutrient solution adding device according to claim 1, it is characterised in that: the fixed feed pipe
(7) lower end through feed opening and extends on the inside of cylinder shape groove.
4. a kind of stem cell nutrient solution adding device according to claim 1, it is characterised in that: the L-type rocking bar (5) is remote
One end from fixed plate (11) is dug from top to bottom threaded hole, and lifting screw (8) are threaded at the threaded hole.
5. a kind of stem cell nutrient solution adding device according to claim 4, it is characterised in that: the lifting screw (8)
Lower end is fixedly connected with sucker (10), and fixing sleeve, which is connected to, in the middle part of the lifting screw (8) inhales ring (9), and inhales ring (9) and be located at L-type
Between rocking bar (5) and sucker (10).
6. a kind of stem cell nutrient solution adding device according to claim 1, it is characterised in that: in the cylindrical type groove
Wall, the first leak hole (14) and the second leak hole (15) inner wall are coated with composite type modified anti-stick coating, the painting of the anti-stick coating
Material includes nanometer non-viscous paint JC316.
7. a kind of stem cell nutrient solution adding device according to claim 1, it is characterised in that: the culture dish lid
(2) side, which is longitudinally carved, is equipped with scale line, and the scale line is equipped with a pair, and a pair of of scale line is symmetrically distributed in culture dish lid
(2) side.
8. a kind of stem cell nutrient solution adding device according to claim 1, it is characterised in that: the annular seal groove
(4) width is greater than the thickness of sealing ring (3), water is placed in the annular seal groove (4), and the liquid level of water is close lower than annular
The notch of sealing groove (4).
9. a kind of stem cell nutrient solution adding device according to claim 8, it is characterised in that: the annular seal groove
(4) inner sidewall is higher than the lateral wall of annular seal groove (4).
10. a kind of stem cell nutrient solution adding device according to claim 1, it is characterised in that: its application method are as follows:
S1, sealing-plug (6) are opened, adds nutrient solution into cylinder shape groove from fixed feed pipe (7)；
S2, Bian Tianjia nutrient solution side level observe scale line, when the liquid level of nutrient solution reaches aequum, stop addition, are stoppered
Sealing-plug (6)；
S3, unscrew lifting screw (8), so that sucker (10) rotates L-type rocking bar (5) manually far from culture dish lid (2) surface,
So that the first leak hole (14) in fixed plate (11) is slowly overlapped with the second leak hole (15) on movable plate (12), cylinder shape groove
In nutrient solution from uniformly being instilled in the gap of coincidence in culture dish ontology (1)；
S4, after being added dropwise, L-type rocking bar (5) are rotated backward, so that the first leak hole (14) and movable plate in fixed plate (11)
(12) the second leak hole (15) on is staggered, and guarantees culture dish ontology (1) internal leakproofness.
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| CN113430241A (en) * | 2021-06-17 | 2021-09-24 | 杨凌质量技术检测检验所 | Food pathogen detection reagent |
| CN113755329A (en) * | 2021-09-23 | 2021-12-07 | 赵宝玉 | Cell culture dish |
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