CN111440716B - Culture device for hair follicle stem cell induced differentiation and use method - Google Patents
Culture device for hair follicle stem cell induced differentiation and use method Download PDFInfo
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- CN111440716B CN111440716B CN202010314145.4A CN202010314145A CN111440716B CN 111440716 B CN111440716 B CN 111440716B CN 202010314145 A CN202010314145 A CN 202010314145A CN 111440716 B CN111440716 B CN 111440716B
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- 210000000130 stem cell Anatomy 0.000 title claims abstract description 69
- 210000003780 hair follicle Anatomy 0.000 title claims abstract description 68
- 230000004069 differentiation Effects 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title claims abstract description 22
- 230000007246 mechanism Effects 0.000 claims abstract description 203
- 238000002347 injection Methods 0.000 claims abstract description 26
- 239000007924 injection Substances 0.000 claims abstract description 26
- 230000001954 sterilising effect Effects 0.000 claims abstract description 21
- 230000001939 inductive effect Effects 0.000 claims abstract description 14
- 241000894006 Bacteria Species 0.000 claims abstract description 13
- 238000001125 extrusion Methods 0.000 claims abstract description 4
- 238000007789 sealing Methods 0.000 claims description 83
- 238000004659 sterilization and disinfection Methods 0.000 claims description 26
- 238000005192 partition Methods 0.000 claims description 15
- 239000004744 fabric Substances 0.000 claims description 13
- 244000309464 bull Species 0.000 claims description 12
- 239000000243 solution Substances 0.000 claims description 12
- 229920001821 foam rubber Polymers 0.000 claims description 10
- 235000015097 nutrients Nutrition 0.000 claims description 10
- 238000003466 welding Methods 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 6
- 235000016709 nutrition Nutrition 0.000 claims description 3
- 230000035764 nutrition Effects 0.000 claims description 3
- 238000005096 rolling process Methods 0.000 claims description 3
- 230000002421 anti-septic effect Effects 0.000 claims description 2
- 230000000149 penetrating effect Effects 0.000 claims description 2
- 230000001737 promoting effect Effects 0.000 claims 1
- 238000003825 pressing Methods 0.000 abstract description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 15
- 238000012258 culturing Methods 0.000 abstract description 6
- 230000003648 hair appearance Effects 0.000 abstract description 5
- 238000004113 cell culture Methods 0.000 abstract description 3
- 230000000694 effects Effects 0.000 description 6
- 239000008223 sterile water Substances 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 230000000249 desinfective effect Effects 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 206010072170 Skin wound Diseases 0.000 description 1
- 210000004504 adult stem cell Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 239000000645 desinfectant Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 210000001732 sebaceous gland Anatomy 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 230000029663 wound healing Effects 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
- C12N5/0627—Hair cells
- C12N5/0628—Hair stem cells; Hair progenitors
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Abstract
The invention relates to the technical field of differentiation culture of hair follicle stem cells, in particular to a culture device for inducing differentiation of hair follicle stem cells and a use method thereof, wherein the culture device comprises a supporting mechanism, a sterilizing mechanism, a moving mechanism, a culture mechanism, an injection mechanism and a screwing mechanism; when the hair follicle stem cells are cultured, the moving mechanism is pushed to realize that the cover screwing mechanism corresponds to one group of culture mechanisms, the cover screwing mechanism is pressed to be buckled with the culture mechanisms, the cover screwing mechanism is rotated to realize that the culture mechanisms are opened and closed, pollution caused by direct contact of hands with a plurality of culture mechanism covers is avoided, bacteria are prevented from entering the culture mechanisms, and the quality of hair follicle stem cell culture and the accuracy of comparison are ensured; when the cap screwing mechanism is pressed, the extrusion of the sterilizing water is realized, and the operation is convenient; the pressing and screwing mechanism realizes that the sterilizing water in the sterilizing mechanism contacts with the unscrewed culturing mechanism, so that the sterilizing of the culturing mechanism is realized, and bacteria are prevented from entering the culturing mechanism during culturing.
Description
Technical Field
The invention relates to the technical field of differentiation culture of hair follicle stem cells, in particular to a culture device for inducing differentiation of hair follicle stem cells and a use method thereof.
Background
Hair follicle stem cells are one of the perifollicular sheath bulge in humans. Hair follicle stem cells belong to adult stem cells, are in a static state in vivo, and show a remarkable proliferation capacity under the in vitro culture effect. It has been found that hair follicle stem cells have multipotency, they can differentiate into epidermis, hair follicle, sebaceous glands, and participate in the process of skin wound healing, and cell induced differentiation is a phenomenon in which a part of cells induce other cells to differentiate in a specific direction, also called embryo induction, and other modes, such as: cascade signals, gradient signals, etc.
At present, when inducing differentiation culture to multiunit hair follicle stem cells, directly put into the inside of blake bottle with hair follicle stem cells, further cultivate to the inside injection nutrient solution of blake bottle, when the inside injection nutrient solution of multiunit blake bottle or take out the inside hair follicle stem cells of multiunit contrast blake bottle, need open the bottle lid, if the bottle lid of a plurality of blake bottles is directly opened with the hand, residual bacterium and bottle lid direct contact in the palm causes the bacterial contamination bottle lid easily, lead to the bacterium to enter into the inside of blake bottle when opening and closing the bottle lid for many times, influence the culture quality of hair follicle stem cells, and need use atomizer to disinfect the bottle when disinfecting a plurality of bottles, be difficult to disinfect at the in-process of twisting the lid, the operation is inconvenient.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a culture device for inducing differentiation of hair follicle stem cells and a use method thereof.
The technical scheme adopted for solving the technical problems is as follows: the utility model provides a culture apparatus that induced differentiation of hair follicle stem cells used, includes supporting mechanism, disinfection mechanism, moving mechanism, cultivates mechanism, injection mechanism and twists lid mechanism, the top sliding connection of supporting mechanism has moving mechanism, the surface equidistance of supporting mechanism is installed and is used for carrying out induced differentiation to hair follicle stem cells cultivate the mechanism, two sets of injection mechanisms that are used for storing the antiseptic solution are installed at moving mechanism's top, moving mechanism's top center department sliding connection has and is located two sets of twist lid mechanism between the injection mechanism, twist lid mechanism's internally mounted has and is used for carrying out sterile disinfection mechanism to cultivateing the mechanism, twist lid mechanism about cultivate the mechanism corresponds the setting, two distance between the injection mechanism is less than twist lid mechanism's maximum diameter.
Specifically, supporting mechanism includes supporting seat and fixed case, mobile mechanism sliding connection in be cuboid mechanism fixed case, fixed case's bottom welding has be cuboid structure the supporting seat.
Specifically, moving mechanism includes support frame, handle, spout and gyro wheel, the surface border department of fixed case has seted up relatively and is "cross" font structure the spout, is the support frame sliding connection of "U" shape structure of falling is in two the inside of spout, install at the both ends of support frame the gyro wheel, two sets of the gyro wheel with rolling connection between the fixed case, two sides of support frame all weld have the lateral wall is the handle of arc structure.
Specifically, culture mechanism includes sealed lid, culture dish, fixed plate and blake bottle, the inside equidistance fixedly connected with of fixed box the blake bottle, a plurality of the inside equal fixedly connected with cross-section of blake bottle is "U" shape structure the fixed plate, a plurality of the culture dish has been placed to the surface of fixed plate inside the blake bottle, a plurality of is located the equal threaded connection in top of blake bottle of fixed box top has the lateral wall to offer the sealed lid of recess, and a plurality of sealed lid all is located between the support frame.
Specifically, twist lid mechanism includes rand, screen cloth, second spring, bull stick, rotary cap, latch, third spring, splint, slider and movable groove, the top center department sliding connection of support frame has the bull stick that is cylindrical structure, the top welding of bull stick has rotary cap, runs through the bottom of support frame bottom the bottom welding of bull stick has the screen cloth, the screen cloth with it holds to press from both sides between the support frame the second spring, the second spring twine in the lateral wall of bull stick, the outside sliding connection of screen cloth has be hollow cylindrical structure, bottom open-ended the rand, the equal distance of inner wall equidistance of rand has seted up the movable groove, a plurality of screen cloth lateral wall the slider all sliding connection in the inside of movable groove, the inner wall of rand is equipped with two sets of the latch that are triangular prism structure relatively, install relatively the inner wall of rand the third spring, two the opposite end fixedly connected with of third spring the splint, the minimum internal diameter of sealed lid equals the sealed lid of rand just be located the rand the bottom of rand the sealed lid.
Specifically, disinfection mechanism includes baffle, disinfection hole and foam-rubber cushion, the inside welding of rand has and is located the baffle of screen cloth bottom, the surface of baffle is annular array and has seted up the disinfection hole, the screen cloth with it holds to press from both sides between the baffle the foam-rubber cushion.
Specifically, injection mechanism includes syringe shell, push rod, clamp plate, first spring and piston, the top of support frame is about bull stick symmetry threaded connection has the syringe shell, two the inside of syringe shell is all sliding connection has the piston, two cross-sections are "T" shape structure the push rod embedding the inside of syringe shell, and two the one end of push rod respectively with two the top fixed connection of piston, and two the syringe shell is located the top of screen cloth, the lateral wall welding of bull stick has the clamp plate that is disc structure, two the push rod is located the bottom of clamp plate syringe shell with the clamp between the push rod has first spring.
Specifically, the using method of the culture device for inducing differentiation of the hair follicle stem cells comprises the following steps:
s1: firstly, protecting articles such as masks are arranged when hair follicle stem cells are induced to differentiate and culture, so that pollution caused by contact of bacteria and hair follicle stem cells during operation is avoided, and a plurality of culture mechanisms are opened through the screwing mechanism;
s2: then taking out a plurality of culture mechanisms, placing hair follicle stem cells into the culture mechanisms, further adding nutrient solution, ensuring that the hair follicle stem cells are cultured in the culture mechanisms, and closing the culture mechanisms through the cover screwing mechanism;
s3: when nutrition liquid is added or contrast is taken out from hair follicle stem cells in the culture mechanism, the culture mechanisms are all opened by pushing the moving mechanism to cooperate with the cap screwing mechanism, the injection mechanism is used for injecting the disinfectant into the cap screwing mechanism when the culture mechanism is opened, and the cap screwing mechanism is extruded to realize that the disinfection mechanism disinfects the culture mechanism and avoids polluting hair follicle stem cells.
The beneficial effects of the invention are as follows:
(1) According to the culture device for the induced differentiation of the hair follicle stem cells and the use method thereof, the top of the supporting mechanism is connected with the moving mechanism in a sliding manner, the top of the moving mechanism is connected with the cover screwing mechanism positioned at the top of the culture mechanism in a sliding manner, when the hair follicle stem cells are cultured, the moving mechanism is pushed to realize that the cover screwing mechanism corresponds to one group of culture mechanisms, the cover screwing mechanism is pressed to be buckled with the culture mechanisms, the cover screwing mechanism is rotated to realize the opening and closing of the culture mechanisms, the pollution caused by direct contact of hands with a plurality of culture mechanism covers is avoided, bacteria are prevented from entering the culture mechanisms, and the quality and the contrast accuracy of the hair follicle stem cell culture are ensured; namely: when the cover is opened on the culture bottles in the plurality of fixed boxes, two handles on the side wall of the support frame are held, the support frame is moved along the two side directions of the fixed boxes, and the idler wheels are moved in the fixed boxes when the support frame moves in the sliding grooves, so that the smooth effect of the support frame during movement is improved, and the support frame is prevented from being blocked when the support frame is moved; the sealing cap is characterized in that the supporting frame is firstly moved when a plurality of sealing caps are opened before culture, the sealing caps which are positioned at the tops of the supporting frames are correspondingly pressed by the clamping rings, then the rotating rods move towards the top direction of the sealing caps, so that the second springs between the screen cloth and the supporting frames are opened, the sliding blocks positioned on the side walls of the screen cloth are further realized to slide in the clamping rings, after the clamping rings are clamped to the sealing caps which are required to be opened, the clamping teeth of the two groups of clamping teeth in the clamping rings are clamped to the grooves of the side walls of the sealing caps, then the rotating rods are rotated anticlockwise, so that the sliding blocks of the side walls of the screen cloth are abutted against the inner walls of the clamping rings, the sealing caps are opened, the opening of the sealing caps is realized, hair follicle stem cells and nutrient solution are added into the culture dish, the rotating rods are loosened, the second springs are reset, the clamping rings are lifted by the clamping rings, then the clamping plates shrink into the sealing caps when the clamping caps are clamped to the sealing caps, and the sealing caps are pulled up by hands, pollution caused by the sealing caps is avoided, after the sealing caps are clamped to the sealing caps, the sealing caps are added into the sealing caps, the sealing caps move towards the top direction of the sealing caps, the sealing caps are pressed again, the sealing caps are correspondingly, the sealing caps are connected to the bottom the sealing caps are required to be closed, and the sealing caps are not in contact with the sealing caps, and the sealing caps are kept in contact with the sealing caps, and the sealing caps are well, and the bottle is closed, and the quality of the bottle is well is realized when the sealing caps are opened, and the bottle cell culture bottle is sealed, and the bottle is sealed, and the bottle is closed.
(2) According to the culture device for inducing differentiation of hair follicle stem cells and the use method, the injection mechanism is connected to the surface of the moving mechanism in a threaded manner, sterilizing water is pumped by the injection mechanism before culture, and further the injection mechanism is extruded by the cap screwing mechanism when the cap screwing mechanism is pressed, so that the sterilizing water is extruded while cap screwing is realized, and the operation is convenient; namely: can control the length of pushing down of bull stick under the prerequisite of guaranteeing latch and sealed lid block when opening sealed lid, then realized can controlling the extrusion degree to the foam-rubber cushion, then realized the clamp plate of bull stick lateral wall and extruded two push rods at the in-process that the bull stick was pushed down to realized pushing the bottom direction of syringe shell with the piston, and then realized extruding the surface of screen cloth to the inside sterile water of syringe shell, convenient operation.
(3) According to the culture device for the induced differentiation of the hair follicle stem cells and the use method thereof, the disinfection mechanism is arranged in the cap screwing mechanism, and the cap screwing mechanism can be pressed when the cap is screwed, so that the disinfection water in the disinfection mechanism is contacted with the unscrewed culture mechanism, the disinfection of the culture mechanism is realized, bacteria are prevented from entering the culture mechanism during the culture, and the culture of the hair follicle stem cells is facilitated; namely: when the rotating rod is extruded, the sponge cushion between the screen and the partition plate is abutted, so that the screen extrudes the sterilizing water inside the sponge cushion, and then the sterilizing water inside the sponge cushion seeps out from the sterilizing holes on the surface of the partition plate, so that the sterilizing water drops to the surface of the sealing cover which is being opened, the sealing cover is sterilized, the sealing cover is prevented from being polluted by bacteria, and the culture of hair follicle stem cells inside the culture flask is facilitated.
Drawings
The invention will be further described with reference to the drawings and examples.
FIG. 1 is a schematic diagram showing the overall structure of a culture apparatus for inducing differentiation of hair follicle stem cells according to a preferred embodiment of the present invention;
FIG. 2 is a schematic view of the connection structure of the support mechanism, the movement mechanism, the training mechanism and the capping mechanism shown in FIG. 1;
FIG. 3 is an enlarged schematic view of the structure A shown in FIG. 2;
FIG. 4 is a schematic view of the capping mechanism shown in FIG. 2;
FIG. 5 is a schematic view of a connection structure between the sterilizing mechanism and the capping mechanism shown in FIG. 4;
FIG. 6 is a schematic view of the capping mechanism shown in FIG. 5;
FIG. 7 is an enlarged schematic view of the portion B shown in FIG. 2;
fig. 8 is a flowchart of a method for using the culture device for inducing differentiation of hair follicle stem cells.
In the figure: 1. the support mechanism, 11, the support seat, 12, the fixed box, 2, the disinfection mechanism, 21, the partition board, 22, the disinfection hole, 23, the foam pad, 3, the moving mechanism, 31, the support frame, 32, the handle, 33, the chute, 34, the roller, 4, the culture mechanism, 41, the sealing cover, 42, the culture dish, 43, the fixed plate, 44, the culture flask, 5, the injection mechanism, 51, the syringe housing, 52, the push rod, 53, the pressing plate, 54, the first spring, 55, the piston, 6, the screwing mechanism, 61, the collar, 62, the screen, 63, the second spring, 64, the rotating rod, 65, the rotating cap, 66, the latch, 67, the third spring, 68, the clamping plate, 69, the slider, 69a and the movable groove.
Detailed Description
The invention is further described in connection with the following detailed description in order to make the technical means, the creation characteristics, the achievement of the purpose and the effect of the invention easy to understand.
As shown in fig. 1-7, the culture device for inducing differentiation of hair follicle stem cells according to the present invention comprises a supporting mechanism 1, a sterilizing mechanism 2, a moving mechanism 3, a culture mechanism 4, an injection mechanism 5 and a cap screwing mechanism 6, wherein the top of the supporting mechanism 1 is slidably connected with the moving mechanism 3, the surface of the supporting mechanism 1 is equidistantly provided with the culture mechanism 4 for inducing differentiation of hair follicle stem cells, the top of the moving mechanism 3 is provided with two groups of injection mechanisms 5 for storing sterilizing liquid, the center of the top of the moving mechanism 3 is slidably connected with the cap screwing mechanism 6 between the two groups of injection mechanisms 5, the sterilizing mechanism 2 for sterilizing the culture mechanism 4 is installed in the cap screwing mechanism 6, the cap screwing mechanism 6 is correspondingly arranged with respect to the culture mechanism 4, and the distance between the two injection mechanisms 5 is smaller than the maximum diameter of the cap screwing mechanism 6.
Specifically, the supporting mechanism 1 includes supporting seat 11 and fixed case 12, mobile mechanism 3 sliding connection in be cuboid mechanism fixed case 12, fixed case 12's bottom welding has be cuboid structure supporting seat 11, fixed case 12 has realized a plurality of the fixation of blake bottle 44, conveniently carries out differentiation culture and contrast to multiunit hair follicle stem cell simultaneously, is located fixed case 12 bottom supporting seat 11 has realized the support to fixed case 12, has increased fixed case 12's stability.
Specifically, the moving mechanism 3 includes a supporting frame 31, a handle 32, a sliding groove 33 and rollers 34, the sliding groove 33 with a cross-shaped structure is relatively provided at the surface edge of the fixed box 12, the supporting frame 31 with an inverted U-shaped structure is slidably connected to the two sliding grooves 33, the rollers 34 are mounted at two ends of the supporting frame 31, the two sets of rollers 34 are in rolling connection with the fixed box 12, the two handles 32 with arc-shaped side walls are welded on the two sides of the supporting frame 31, when the two handles 32 are used for opening the cover of the culture bottles 44 in the fixed box 12, the two handles 32 are used for holding the side walls of the supporting frame 31, the supporting frame 31 is moved along the two side directions of the fixed box 12, and the rollers 34 are moved in the fixed box 12 when the supporting frame 31 is moved in the fixed box 12, so that smooth effect when the supporting frame 31 is moved is increased, and the supporting frame 31 is prevented from being blocked when the supporting frame 31 is moved.
Specifically, the culture mechanism 4 includes sealed lid 41, culture dish 42, fixed plate 43 and blake bottle 44, the inside equidistance fixedly connected with of fixed box 12 blake bottle 44, a plurality of the inside of blake bottle 44 is all fixedly connected with cross-section is "U" shape structure fixed plate 43, a plurality of the inside the surface of fixed plate 43 of blake bottle 44 has been placed the culture dish 42, a plurality of be located the top of fixed box 12 the top of blake bottle 44 is all threaded connection has the lateral wall to set up the sealed lid 41 of recess, and a plurality of sealed lid 41 all is located between the support frame 31, after a plurality of sealed lid 41 is opened, with multiunit hair follicle stem cell is added the inside of blake bottle 44 the inside of blake dish 42, further to a plurality of the inside of blake bottle 42 is added the nutrient solution, guarantees hair follicle stem cell normal survival, then will a plurality of the blake bottle 44 top the surface is placed the sealed lid 41, avoids the bacterium to get into the inside of blake bottle 44 pollutes the hair follicle stem cell, guarantees the stem cell of stem cell quality of hair follicle stem cell.
Specifically, the capping mechanism 6 includes a collar 61, a screen 62, a second spring 63, a rotating rod 64, a rotating cap 65, a latch 66, a third spring 67, a clamping plate 68, a sliding block 69, and a movable slot 69a, the rotating rod 64 having a cylindrical structure is slidingly connected to the top center of the supporting frame 31, the rotating cap 65 is welded to the top of the rotating rod 64, the screen 62 is welded to the bottom of the rotating rod 64 penetrating the bottom of the supporting frame 31, the second spring 63 is clamped between the screen 62 and the supporting frame 31, the second spring 63 is wound around the side wall of the rotating rod 64, the collar 61 having a hollow cylindrical structure and an opening at the bottom is slidingly connected to the outer side of the screen 62, the movable slot 69 is vertically provided on the inner wall of the collar 61 at equal intervals, the sliding blocks 69 on the side wall of the screen 62 are slidingly connected to the inner portion of the movable slot 69a, the inner wall of the collar 61 is relatively provided with two groups of clamping teeth 66 in a triangular prism structure, the inner wall of the collar 61 is relatively provided with a third spring 67, opposite ends of the two third springs 67 are fixedly connected with clamping plates 68, the minimum inner diameter of the collar 61 is equal to the maximum diameter of the sealing cover 41, the sealing cover 41 is positioned at the bottom of the collar 61, the collar 61 is buckled on the side wall of the sealing cover 41, when a plurality of sealing covers 41 are opened before culturing, the supporting frame 31 is firstly moved, the collar 61 corresponds to the sealing cover 41 which needs to be opened, the rotating cap 65 positioned at the top of the supporting frame 31 is further pressed, the rotating rod 64 moves towards the top direction of the sealing cover 41, thereby opening the second spring 63 positioned between the screen mesh 62 and the supporting frame 31, further realizing that the sliding block 69 positioned on the side wall of the screen 62 slides in the inner part of the clamping ring 31, when the clamping ring 31 is clamped to the sealing cover 41 which needs to be opened, realizing that two groups of clamping teeth 66 in the inner part of the clamping ring 31 are clamped with grooves on the side wall of the sealing cover 41, then rotating the rotating rod 64 anticlockwise at the moment realizes that the sliding block 69 on the side wall of the screen 62 collides with the inner wall of the clamping ring 31, thereby realizing the opening of the sealing cover 41, loosening the rotating rod 64 realizes that the second spring 63 is reset, the clamping ring 31 is lifted, then the clamping plate 68 is contracted in the inner part of the clamping ring 31 when the clamping ring 31 is clamped with the sealing cover 41, and two groups of third springs 67 are tensioned on the clamping plate 68, thereby realizing that the sealing cover 41 is replaced by the sealing cover 41 when the sealing cover 31 is lifted, avoiding pollution caused by taking the sealing cover 41 by hand, after adding hair follicle stem cells and nutrient solution into the culture dish 42, pressing the rotary cap 65 again to realize that the clamping ring 31 moves towards the top of the culture flask 44, corresponding the sealing cover 41 at the bottom of the clamping ring 31 to the opening of the culture flask 44 to be closed, rotating the rotary rod 64 clockwise to realize that the sealing cover 41 is connected with the top of the culture flask 44 in a threaded manner, loosening the rotary rod 64 to realize that the clamping ring 31 is separated from the covered sealing cover 41, thereby opening and closing the rest of the sealing covers 41 according to the method, avoiding bacterial pollution to the sealing cover 41 when the culture dish 42 and the culture flask 44 are not contacted with hands of experimenters during culture, the culture quality of the hair follicle stem cells is ensured.
Specifically, the disinfection mechanism 2 includes a partition 21, a disinfection hole 22 and a foam-rubber cushion 23, the partition 21 located at the bottom of the screen 62 is welded in the collar 61, the disinfection hole 22 is formed on the surface of the partition 21 in a ring array, the foam-rubber cushion 23 is clamped between the screen 62 and the partition 21, when the rotating rod 64 is pressed, the foam-rubber cushion 23 between the screen 62 and the partition 21 is abutted, so that the disinfection water inside the foam-rubber cushion 23 is extruded by the screen 62, and then the disinfection water inside the foam-rubber cushion 23 seeps out from the disinfection hole 22 on the surface of the partition 21, so that disinfection water drops on the surface of the sealing cover 41 which is being opened, the sealing cover 41 is disinfected, bacterial pollution is avoided to the sealing cover 41, and the cultivation of hair follicle stem cells inside the culture bottle 44 is facilitated.
Specifically, the injection mechanism 5 includes a syringe housing 51, a push rod 52, a pressing plate 53, a first spring 54 and a piston 55, the top of the supporting frame 31 is symmetrically screwed with the syringe housing 51 about the pressing rod 64, the two inside of the syringe housing 51 are both slidably connected with the piston 55, the two push rods 52 with a T-shaped cross section are embedded into the inside of the syringe housing 51, one ends of the two push rods 52 are respectively and fixedly connected with the top of the two pistons 55, the two syringe housings 51 are located at the top of the screen 62, the pressing plate 53 with a disc-shaped structure is welded on the side wall of the pressing rod 64, the two push rods 52 are located at the bottom of the pressing plate 53, the first spring 54 is clamped between the syringe housing 51 and the pushing rod 52, when the sealing cover 41 is opened, the length of the pressing down of the pressing rod 64 is controlled on the premise that the clamping teeth 66 and the sealing cover 41 are clamped is guaranteed, the sealing cover 23 can be controlled, the sponge cushion 23 can be pressed down, the pressing plate 53 is further pressed down to the side wall of the sponge housing 64, and the sponge housing 52 is pressed down, and the side wall of the sponge housing is pressed down by the pressing plate 52 is further, and the sponge housing 52 is pressed down, and the side wall of the sponge housing is pressed down by the sponge housing 52.
Specifically, referring to fig. 8 in combination, fig. 8 is a flowchart of a method for using the culture device for inducing differentiation of hair follicle stem cells according to the present invention, and the method for using the culture device for inducing differentiation of hair follicle stem cells comprises the following steps:
s1: firstly, protecting articles such as masks are arranged when hair follicle stem cells are induced to differentiate and culture, so that pollution caused by contact of bacteria and hair follicle stem cells during operation is avoided, and a plurality of culture mechanisms 4 are opened through the screwing mechanism 6;
s2: then, taking out a plurality of culture mechanisms, placing hair follicle stem cells into the culture mechanism 4, further adding nutrient solution, ensuring that the hair follicle stem cells are cultured in the culture mechanism 4, and closing the culture mechanisms 4 through the screwing mechanism 6;
s3: when nutrition liquid is added or contrast is taken out from hair follicle stem cells in the culture mechanism 4, the plurality of culture mechanisms 4 are all opened by pushing the moving mechanism 3 to cooperate with the cap screwing mechanism 6, the injection mechanism 5 is used for injecting disinfection liquid into the cap screwing mechanism 6 when the culture mechanism 4 is opened, the cap screwing mechanism 6 is extruded, the disinfection mechanism 2 is used for disinfecting the culture mechanism 4, and pollution to hair follicle stem cells is avoided.
When the invention is used, firstly, the fixing of a plurality of culture flasks 44 is realized through the fixing box 12, the differentiation culture and comparison of a plurality of groups of hair follicle stem cells are conveniently carried out at the same time, the support seat 11 positioned at the bottom of the fixing box 12 supports the fixing box 12, the stability of the fixing box 12 is increased, when the culture flasks 44 in the fixing boxes 12 are uncapped, two handles 32 on the side wall of the support frame 31 are held, the support frame 31 is moved along the two side directions of the fixing box 12, and when the support frame 31 is moved in the sliding groove 33, the roller 34 is moved in the fixing box 12, the smooth effect of the support frame 31 in the moving process is increased, and the support frame 31 is prevented from being blocked when the support frame 31 is moved; then, when the plurality of sealing covers 41 are opened before culturing, the supporting frame 31 is moved firstly, the effect that the clamping ring 61 corresponds to the sealing cover 41 which needs to be opened is achieved, the rotating cap 65 positioned at the top of the supporting frame 31 is further pressed, then the rotating rod 64 moves towards the top direction of the sealing cover 41, so that the second spring 63 positioned between the screen 62 and the supporting frame 31 is opened, further the sliding block 69 positioned on the side wall of the screen 62 slides in the clamping ring 31, after the clamping ring 31 is clamped to the sealing cover 41 which needs to be opened, the two groups of clamping teeth 66 in the clamping ring 31 are clamped with the grooves on the side wall of the sealing cover 41 are achieved, at the moment, the rotating rod 64 is rotated anticlockwise, the sliding block 69 on the side wall of the screen 62 is abutted against the inner wall of the clamping ring 31, the sealing cover 41 is opened, the second spring 63 is reset is achieved by loosening the rotating rod 64, the clamping ring 31 is lifted, then clamping plate 68 is contracted into the inside of clamping plate 31 when clamping plate 31 is clamped with sealing cover 41, and two third springs 67 have tension on clamping plate 68, so that sealing cover 41 is replaced together when clamping plate 31 is lifted, pollution caused by taking sealing cover 41 by hand is avoided, when a plurality of sealing covers 41 are opened, a plurality of groups of hair follicle stem cells are added into the inside of culture dish 42 in culture flask 44, further nutrient solution is added into the inside of a plurality of culture dishes 42, normal survival of hair follicle stem cells is ensured, after hair follicle stem cells and nutrient solution are added into the inside of culture dish 42, rotating cap 65 is pressed again to realize that clamping plate 31 moves towards the top of culture flask 44, sealing cover 41 at the bottom of clamping plate 31 corresponds to the opening of culture flask 44 to be closed, rotating rod 64 is rotated clockwise to realize that sealing cover 41 is connected to the top of culture flask 44 in a threaded manner, loosening the rotating rod 64 realizes the separation of the clamping ring 31 and the covered sealing cover 41, so that the rest sealing covers 41 are opened and closed according to the method, thereby realizing that the culture dish 42 and the culture bottle 44 are not contacted with hands of an experimenter during culture, avoiding bacteria from polluting the sealing covers 41, and ensuring the culture quality of hair follicle stem cells; finally, the length of the pressing down of the rotating rod 64 can be controlled on the premise that the clamping teeth 66 are clamped with the sealing cover 41 when the sealing cover 41 is opened, so that the extrusion degree of the sponge cushion 23 can be controlled, the pressing plates 53 on the side walls of the rotating rod 64 can extrude the two pushing rods 52 in the pressing down process of the rotating rod 64, the piston 55 can be pushed towards the bottom of the syringe housing 51, the sterile water inside the syringe housing 51 can be extruded to the surface of the screen 62 and then absorbed by the sponge cushion 23, the sponge cushion 23 between the screen 62 and the partition plate 21 can be pushed against when the rotating rod 64 is extruded, the sterile water inside the sponge cushion 23 can be extruded out from the sterilizing holes 22 on the surface of the partition plate 21, the sterile water drops can be dropped onto the surface of the sealing cover 41 which is being opened, the sealing cover 41 can be sterilized, the bacteria can be prevented from polluting the sealing cover 41, and the culture of hair follicle stem cells inside the culture bottle 44 can be facilitated.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present disclosure describes embodiments, not every embodiment is provided with a separate embodiment, and that this description is provided for clarity only, and that the disclosure is not limited to the embodiments described in detail below, and that the embodiments described in the examples may be combined as appropriate to form other embodiments that will be apparent to those skilled in the art.
Claims (2)
1. The culture device for inducing differentiation of hair follicle stem cells is characterized by comprising a supporting mechanism (1), a sterilizing mechanism (2), a moving mechanism (3), a culture mechanism (4), an injection mechanism (5) and a screwing mechanism (6), wherein the top of the supporting mechanism (1) is connected with the moving mechanism (3) in a sliding manner, the culture mechanism (4) for inducing differentiation of hair follicle stem cells is arranged on the surface of the supporting mechanism (1) at equal intervals, two groups of injection mechanisms (5) for storing sterilizing liquid are arranged on the top of the moving mechanism (3), the screwing mechanism (6) arranged between the two groups of injection mechanisms (5) is connected in a sliding manner at the center of the top of the moving mechanism (3), the sterilizing mechanism (2) for sterilizing the culture mechanism (4) is arranged in the screwing mechanism (6), the screwing mechanism (6) is correspondingly arranged relative to the culture mechanism (4), and the distance between the two injection mechanisms (5) is smaller than the maximum diameter of the screwing mechanism (6);
the supporting mechanism (1) comprises a supporting seat (11) and a fixed box (12), the moving mechanism (3) is connected with the fixed box (12) which is a cuboid mechanism in a sliding manner, and the supporting seat (11) which is a cuboid structure is welded at the bottom of the fixed box (12);
the moving mechanism (3) comprises a supporting frame (31), a handle (32), sliding grooves (33) and rollers (34), the sliding grooves (33) which are of a cross-shaped structure are oppositely formed in the edge of the surface of the fixed box (12), the supporting frame (31) which is of an inverted U-shaped structure is connected to the inner parts of the two sliding grooves (33) in a sliding mode, the rollers (34) are arranged at two ends of the supporting frame (31), the two groups of rollers (34) are in rolling connection with the fixed box (12), and the handles (32) with arc-shaped side walls are welded on two side faces of the supporting frame (31);
the culture mechanism (4) comprises a sealing cover (41), a culture dish (42), a fixing plate (43) and culture bottles (44), wherein the culture bottles (44) are fixedly connected to the inside of the fixed box (12) at equal intervals, the fixing plates (43) with U-shaped cross sections are fixedly connected to the inside of the culture bottles (44), the culture dish (42) is placed on the surface of each fixing plate (43) inside the culture bottles (44), the sealing covers (41) with grooves formed in the side walls are connected to the tops of the culture bottles (44) and are arranged on the tops of the fixed box (12) in a threaded mode, and the sealing covers (41) are arranged between the supporting frames (31);
the screwing mechanism (6) comprises a clamping ring (61), a screen (62), a second spring (63), a rotating rod (64), a rotating cap (65), a clamping tooth (66), a third spring (67), a clamping plate (68), a sliding block (69) and a movable groove (69 a), wherein the rotating rod (64) with a cylindrical structure is slidingly connected to the center of the top of the supporting frame (31), the rotating cap (65) is welded to the top of the rotating rod (64), the screen (62) is welded to the bottom of the rotating rod (64) penetrating through the bottom of the supporting frame (31), the second spring (63) is clamped between the screen (62) and the supporting frame (31), the second spring (63) is wound on the side wall of the rotating rod (64), the clamping ring (61) with a hollow cylindrical structure and an opening at the bottom is slidingly connected to the outer side of the screen (62), the sliding groove (69) is vertically arranged on the inner wall of the clamping ring (61) with equal distance, the sliding blocks (69) on the side wall of the screen (62) are slidingly connected to the inner side wall (69) of the clamping ring (69), the clamping ring (69) is provided with a three opposite to the inner wall (61) of the clamping ring (61), the opposite ends of the two third springs (67) are fixedly connected with clamping plates (68), the minimum inner diameter of the clamping ring (61) is equal to the maximum diameter of the sealing cover (41), the sealing cover (41) is positioned at the bottom of the clamping ring (61), and the clamping ring (61) is buckled on the side wall of the sealing cover (41);
the disinfection mechanism (2) comprises a partition board (21), disinfection holes (22) and a foam-rubber cushion (23), wherein the partition board (21) positioned at the bottom of the screen (62) is welded in the clamping ring (61), the surface of the partition board (21) is provided with the disinfection holes (22) in an annular array, and the foam-rubber cushion (23) is clamped between the screen (62) and the partition board (21);
injection mechanism (5) are including syringe shell (51), push rod (52), clamp plate (53), first spring (54) and piston (55), the top of support frame (31) is about bull stick (64) symmetry threaded connection has syringe shell (51), two the inside of syringe shell (51) all sliding connection has piston (55), two cross-section are "T" shape structure push rod (52) imbeds the inside of syringe shell (51), and two the one end of push rod (52) respectively with two the top fixed connection of piston (55), and two syringe shell (51) are located the top of screen cloth (62), the lateral wall welding of bull stick (64) has be disc structure clamp plate (53), two push rod (52) are located the bottom of clamp plate (53) syringe shell (51) with it holds between push rod (52) first spring (54).
2. The method of claim 1, wherein the method comprises the steps of: the using method comprises the following steps:
s1: firstly, protecting articles such as masks are taken when hair follicle stem cells are induced to differentiate and culture, so that pollution caused by contact of bacteria and hair follicle stem cells during operation is avoided, and a plurality of culture mechanisms (4) are opened through the screwing mechanism (6);
s2: then taking out a plurality of culture mechanisms, placing hair follicle stem cells into the culture mechanisms (4), further adding nutrient solution, and ensuring that the hair follicle stem cells are cultured in the culture mechanisms (4), and closing a plurality of culture mechanisms (4) through the cover screwing mechanism (6);
s3: when nutrition liquid is added or contrast is taken out to the hair follicle stem cells inside culture mechanism (4), through promoting mobile mechanism (3) cooperation twist lid mechanism (6) are all opened to a plurality of culture mechanism (4), realize when opening culture mechanism (4) injection mechanism (5) is with the antiseptic solution injection twist lid mechanism (6) inside, extrusion twist lid mechanism (6) has realized disinfection mechanism (2) are right culture mechanism (4) disinfection avoids polluting hair follicle stem cells.
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| CN116463216B (en) * | 2023-04-23 | 2024-08-27 | 上海鲁一细胞生物技术有限公司 | Stem cell preparation tank and process |
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| JP2008237203A (en) * | 2007-03-27 | 2008-10-09 | J Tec:Kk | Automatic cell-culturing apparatus |
| CN102586177A (en) * | 2012-02-15 | 2012-07-18 | 北京雍禾植发技术研究院 | Culture method of hair follicle stem cell for treating baldness, as well as syringe |
| CN110331095A (en) * | 2019-06-11 | 2019-10-15 | 华夏源（上海）细胞基因工程股份有限公司 | A kind of stem cell nutrient solution adding device and its application method |
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| KR100946643B1 (en) * | 2007-11-30 | 2010-03-09 | 코아스템(주) | The cell culture apparatus and mass automatic cell culture device having it |
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|---|---|---|---|---|
| JP2008237203A (en) * | 2007-03-27 | 2008-10-09 | J Tec:Kk | Automatic cell-culturing apparatus |
| CN102586177A (en) * | 2012-02-15 | 2012-07-18 | 北京雍禾植发技术研究院 | Culture method of hair follicle stem cell for treating baldness, as well as syringe |
| CN110331095A (en) * | 2019-06-11 | 2019-10-15 | 华夏源（上海）细胞基因工程股份有限公司 | A kind of stem cell nutrient solution adding device and its application method |
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