CN212357264U

CN212357264U - Immune cell culture device

Info

Publication number: CN212357264U
Application number: CN202020731822.8U
Authority: CN
Inventors: 付康
Original assignee: Henan Intercell Biology Technology Co ltd
Current assignee: Henan Intercell Biology Technology Co ltd
Priority date: 2020-05-07
Filing date: 2020-05-07
Publication date: 2021-01-15
Anticipated expiration: 2030-05-07

Abstract

The utility model relates to an immune cell culture device, including the sealed tank body and sterilization case, the intercommunication has first connecting pipe between the sealed tank body and the sterilization case, the lower part of sealed tank body one side is equipped with the gas vent, sealed tank body top is connected with the lid, the inside culture tank that is equipped with of sealed tank, the outside both sides of culture tank are fixed on the inner wall of the sealed tank body, culture tank top opening sets up, the culture tank bottom is connected with the lower cover, be equipped with the discharge opening on the lower cover, the lateral wall of culture tank is inside hollow bilayer structure, be outer wall and inner wall respectively, spiral winding has the heat source pipe on the inner wall, the upper and lower both ends of heat source pipe all extend to the outside of the sealed tank body, the top of lid is equipped with the bellows, the bottom mounting of bellows is to the inside sampling tube that extends of culture tank, sampling tube and bellows intercommunication, one side of sampling tube is. The cell sap in the culture tank can be sucked out by squeezing the corrugated pipe, and the tank door does not need to be opened, so that the sampling is convenient.

Description

Immune cell culture device

Technical Field

The utility model belongs to the technical field of cell culture, especially, relate to an immune cell culture device.

Background

With the development of science and technology, biological immunotherapy becomes an emerging means for treating cancer, is a key technology in the technical field of biological therapy, and has good research and development and application prospects. The biological immunotherapy is a treatment method which extracts immune cells in a patient body by a modern medical method, and then the immune cells are returned to the patient body after in vitro culture, proliferation and activation in sequence so as to induce the human body to generate antiviral immune response. In the whole treatment process, the in vitro culture of the immune cells is an important component for realizing the technical scheme, and the reasonable design of the immune cell culture device is a key factor for realizing the safe and effective amplification of the immune cells.

In immune cell's cultivation in-process, in order to facilitate timely understanding the state of cultivation of cell, need set up a plurality of sampling time points and come the growth to the cell, the division, the proliferation process is monitored, current culture apparatus is when carrying out the sample monitoring, often need open the culture tank door, directly stretch into the culture tank with straw or syringe and take a sample, for preventing that the cell in the culture tank is contaminated, this sampling process must go on under the aseptic condition completely, and need carry out thorough sterilization process to various consumptive materials in advance, whole process is loaded down with trivial details, consuming time, bring very big trouble for operating personnel.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing an immune cell culture device to solve the inconvenient technical problem of current immune cell culture device sample.

In order to solve the technical problem, the utility model discloses the technical scheme who adopts is:

provides an immune cell culture device, which comprises a sealed tank body and a sterilization box which is arranged on one side of the sealed tank body and is used for killing bacteria contained in the air, wherein a first connecting pipe is communicated between the sealed tank body and the sterilization box, the first connecting pipe extends to the upper part inside the sealed tank body, an exhaust port for discharging waste gas is arranged on the lower part on one side of the sealed tank body, a second connecting pipe is connected with the exhaust port, a cover body is detachably and fixedly connected with the top of the sealed tank body, a culture tank for containing cells and culture solution is arranged inside the sealed tank body, the culture tank is positioned below the first connecting pipe, fixing rods which are symmetrically arranged are fixedly connected on two sides of the outside of the culture tank, one end of each fixing rod, which deviates from the culture tank, is fixedly connected on the inner wall of the sealed tank body, the top opening of the culture tank is, the utility model discloses a culture tank, including the sealed jar body, the upper and lower both ends of sealed jar body are equipped with the bellows, the bellows is equipped with the bottom, the upper and lower both ends of bellows are equipped with the bellows, the discharge opening has been seted up on the lower cover, discharge opening department is connected with downwardly extending's discharging pipe, the discharging pipe runs through the bottom of the sealed jar body and extends to the sealed jar body externally, and the lateral wall of cultivateing the jar is inside hollow bilayer structure, is outer wall and inner wall respectively, spiral winding has the heat source pipe on the inner wall, the upper and lower both ends of heat source pipe all run through the lateral wall of the outer wall and the sealed jar body and extend to the outside of the sealed jar body, the top of lid is equipped with the bellows.

The top fixedly connected with mounting bracket of lid, the top fixedly connected with pneumatic cylinder of mounting bracket, the vertical downward setting of output of pneumatic cylinder, the bottom fixedly connected with movable rod of pneumatic cylinder output, the movable rod extends to the inside of culture tank and fixedly connected with is used for the circular disk with cell and culture solution mixing, set up the water hole of crossing that a plurality of even intervals set up on the circular disk.

The sterilizing box comprises a box body, an air blower is arranged on one side of the box body, a third connecting pipe is communicated between the air blower and the box body, an air outlet is formed in one side, away from the air blower, of the box body, one end of the first connecting pipe is connected to the air outlet, a plurality of baffle plates which are arranged in a staggered mode are fixedly connected to the inner wall of the box body, the baffle plates are arranged in a downward inclined mode, an ultraviolet lamp tube is arranged between two adjacent baffle plates located on the same side of the inner wall of the box body, and the ultraviolet lamp tube.

The end part of one end of the first connecting pipe extending into the interior of the sealing tank body is sealed, and a plurality of vent holes are formed in the first connecting pipe located in the interior of the sealing tank body.

One side of the sealed tank body is provided with a beaker filled with sterilized water, and one end of the second connecting pipe, which is far away from the sealed tank body, is positioned at the lower part of the liquid level of the sterilized water.

The ultraviolet lamp tube is a bent tube which rotates in a bow shape.

One side of the sealed tank body is hinged with a movable door, and one side of the movable door and the sealed tank body are provided with door lock mechanisms for locking the movable door.

The corrugated pipe is made of elastic rubber.

Rubber sealing rings are sleeved at the joint of the sampling tube and the cover body and the joint of the output end of the hydraulic cylinder and the cover body.

The utility model has the advantages that: (1) when sampling is needed, the corrugated pipe can be held by hand and extruded, cell liquid in the culture tank enters the sampling pipe under the action of pressure difference and is discharged by the liquid outlet pipe, a tank door does not need to be opened, sampling is convenient, the internal environment of the culture tank can be in a continuous and stable state, growth and proliferation of immune cells are facilitated, and the immune cell culture effect is improved; (2) the constant temperature heat source is introduced into the heat source pipe, so that the temperature inside the culture tank is kept constant, a good growth environment is provided for immune cells, the growth speed of the immune cells is accelerated, and the culture period of the immune cells is shortened; (3) by arranging the hydraulic cylinder and the circular disc, the circular disc moves up and down in the culture tank along with the expansion of the output end of the hydraulic cylinder, and the cell sap is mixed through the water through holes, so that the uniformity of sampling is ensured; (4) the sterilizing box is used for sterilizing and disinfecting the air entering the culture tank, so that immune cells are prevented from being infected by external germs, the stay time of the air in the sterilizing box is prolonged by arranging the baffle plate, the ultraviolet lamp tube is arranged into the bent tube which rotates in a bow shape, the area of the ultraviolet lamp tube in a unit area is increased, the sterilization and disinfection are more thorough, and the sterilizing effect is improved; (5) a movable door is hinged to one side of the sealed tank body, and before or after cell culture work begins, the movable door is convenient to open to sterilize and disinfect the inside of the sealed tank body, so that the cleanliness of the inside of the sealed tank body is ensured; (6) the end part of the second connecting pipe is sealed by the sterilized water, so that the second connecting pipe is prevented from being directly exposed to the external environment and being polluted.

Drawings

Fig. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic structural view of a sealed can body;

FIG. 3 is a schematic view of the structure of a culture tank;

fig. 4 is a schematic structural diagram of the ultraviolet lamp tube.

Reference numerals: 1-sealed tank body, 2-sterilization box, 3-supporting leg, 4-first connecting pipe, 5-vent hole, 6-vent hole, 7-second connecting pipe, 8-beaker, 9-cover body, 10-culture tank, 11-fixed rod, 12-lower cover, 13-discharge hole, 14-discharge pipe, 15-outer wall, 16-inner wall, 17-heat source pipe, 18-corrugated pipe, 19-sampling pipe, 20-discharge pipe, 21-mounting rack, 22-hydraulic cylinder, 23-circular disk, 24-water through hole, 25-rubber sealing ring, 26-movable door, 27-door lock mechanism, 28-box body, 29-blower, 30-third connecting pipe, 31-air outlet, 32-baffle plate, 33-ultraviolet lamp tube, 34-valve and 35-movable rod.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention clearer, embodiments of the present invention are described in further detail below with reference to the accompanying drawings.

As shown in fig. 1, the utility model discloses a sealed tank body 1 and the sterilization case 2 that is arranged in killing the bacterium that contains in the air of setting up in sealed tank body 1 one side, the landing leg 3 that the bottom fixedly connected with symmetry of sealed tank body 1 set up, it has first connecting pipe 4 to communicate between sealed tank body 1 and the sterilization case 2, first connecting pipe 4 extends to the inside top of sealed tank body 1, first connecting pipe 4 stretches into the inside one end seal setting of sealed tank body 1, be located and seted up a plurality of ventilation holes 5 on the first connecting pipe 4 of sealed tank body 1 inside, clean air in the first connecting pipe 4 disperses on the upper portion of sealed tank body 1 behind ventilation hole 5, gas impact to immunocyte has been reduced on the one hand, the area of contact of culture solution in clean air and the culture tank 10 has also been increased in addition, be favorable to immunocyte's growth. An exhaust port 6 used for discharging waste gas is formed in the lower portion of one side of the sealed tank body 1, a second connecting pipe 7 is connected to the exhaust port 6, a beaker 8 filled with sterilizing water is arranged on one side of the sealed tank body 1, and one end, deviating from the sealed tank body 1, of the second connecting pipe 7 is located on the lower portion of the liquid level of the sterilizing water. Cell metabolism waste gas that produces in the sealed jar body 1 gets into beaker 8 through second connecting pipe 7 in, the tip of second connecting pipe 7 is sealed by the sterilization water liquid, avoids second connecting pipe 7 directly to expose in the external environment by the pollution. The top threaded connection of the sealed tank body 1 has a lid 9, the inside of the sealed tank body 1 is equipped with the culture tank 10 that is used for holding cell and culture solution, culture tank 10 is located the below of first connecting pipe 4, the dead lever 11 that the outside both sides fixedly connected with symmetry of culture tank 10 set up, dead lever 11 deviates from the one end fixed connection of culture tank 10 on the inner wall of the sealed tank body 1, the open-top setting of culture tank 10, the bottom threaded connection of culture tank 10 has lower cover 12, discharge opening 13 has been seted up on lower cover 12, discharge opening 13 department is connected with downwardly extending's discharging pipe 14, discharging pipe 14 runs through the bottom of the sealed tank body 1 and extends to the outside of the sealed tank body 1. The side wall of the culture tank 10 is a double-layer structure with a hollow inner part, and the double-layer structure is respectively an outer wall 15 and an inner wall 16, as shown in fig. 3, a heat source pipe 17 is spirally wound on the inner wall 16, the upper end and the lower end of the heat source pipe 17 both penetrate through the outer wall 15 and the side wall of the sealed tank body 1 and extend to the outside of the sealed tank body 1, and constant-temperature clean water is introduced into the heat source pipe 17. The top of lid 9 is equipped with bellows 18, and the material of bellows 18 is elastic rubber, and bellows 18 is inside hollow structure, and bellows 18's bottom fixed connection is to the sampling tube 19 of the inside extension of culture tank 10, and sampling tube 19 is linked together with bellows 18, and one side fixedly connected with of sampling tube 19 has the drain pipe 20 of the downward sloping setting that is linked together with sampling tube 19. The top fixedly connected with mounting bracket 21 of lid 9, the top fixedly connected with pneumatic cylinder 22 of mounting bracket 21, the vertical downward setting of output of pneumatic cylinder 22, the bottom fixedly connected with movable rod 35 of the output of pneumatic cylinder 22, inside and fixedly connected with that movable rod 35 extends to culture tank 10 are used for the circular dish 23 with cell and culture solution mixing, set up the water hole 24 of crossing that a plurality of even intervals set up on the circular dish 23. The connection of the sampling tube 19 and the cover 9 and the connection of the output end of the hydraulic cylinder 22 and the cover 9 are all sleeved with rubber sealing rings 25 to ensure the air tightness of the sealed tank body 1. As shown in figure 2, one side of the sealed tank body 1 is hinged with a movable door 26, one side of the movable door 26 and the sealed tank body 1 are provided with door lock mechanisms 27 for locking the movable door 26, and before or after the cell culture work is started, the movable door 26 is opened to sterilize and disinfect the inside of the sealed tank body 1, so that the cleanliness of the inside of the sealed tank body 1 is ensured.

As shown in fig. 1, the sterilization box 2 includes a box body 28, an air blower 29 is disposed on one side of the box body 28, a third connecting pipe 30 is communicated between an air outlet of the air blower 29 and the box body 28, an air outlet 31 is disposed on one side of the box body 28 departing from the air blower 29, one end of the first connecting pipe 4 is connected to the air outlet 31, a plurality of baffle plates 32 which are arranged in a staggered manner are fixedly connected to an inner wall of the box body 28, the baffle plates 32 are arranged in a downward inclined manner, an ultraviolet lamp tube 33 is disposed between two adjacent baffle plates 32 which are located on the same side of the inner wall of the box body 28, and the ultraviolet lamp tube 33 is fixedly connected to the inner wall of the box body 28, as shown in fig. 4, the ultraviolet lamp tube 33 is a bent pipe which is in.

Valves 34 are arranged at the joint of the first connecting pipe 4 and the air outlet 31, the joint of the second connecting pipe 7 and the sealed tank body 1, the joint of the third connecting pipe 30 and the air blower 29, the end parts of the upper end and the lower end of the heat source pipe 17, the liquid outlet pipe 20 and the discharge pipe 14.

The technical solution can be realized by selecting a blower and a door lock mechanism commonly used in the prior art, and therefore, the specific structures of the blower 29 and the door lock mechanism 27 described in this embodiment will not be described in detail herein.

The utility model discloses when using, open valve 34 on the third connecting pipe 30, air among the external environment gets into sterilization box 2 through air-blower 29 in, disinfect under ultraviolet fluorescent tube 33's effect, sterilization treatment, open valve 34 on the first connecting pipe 4, clean air gets into the internal 1 inside of sealed tank, open valve 34 on the second connecting pipe 7, cell growth, waste gas that the metabolic process produced gets into beaker 8 through second connecting pipe 7 in, the tip of second connecting pipe 7 is sealed by the sterilization water, avoid second connecting pipe 7 directly to be exposed to by the pollution in the external environment. Provides continuous fresh and clean air for the culture tank 10 and provides a good growth environment for immune cells. The valves 34 provided at the upper and lower ends of the heat source pipe 17 are opened, and clean water of constant temperature is supplied into the heat source pipe 17 to keep the temperature inside the culture tank 10 constant. When the observation that needs the sample, start hydraulic cylinder 22, thereby the output up-and-down motion of hydraulic cylinder 22 drives circular disk 23 up-and-down motion, and the cell liquid is then mixed through water hole 24, guarantees the homogeneity of sample. The part that exposes in the air of drain pipe 20 is disinfected with 75% medical alcohol earlier before the sample, then open valve 34 on the drain pipe 20, hold bellows 18 and extrude it, the cell sap in the culture tank 10 gets into sampling tube 19 and discharges by drain pipe 20 under the effect of pressure differential, need not open the jar door, it is convenient to sample, and can make the internal environment of culture tank 10 be in a state that lasts stable, be favorable to immunocyte's growth, the hyperplasia, improve the effect that the immunocyte was cultivateed. After sampling, valve 34 on outlet pipe 20 is closed in time, and the exposed part of outlet pipe 20 in the air is sterilized again. After the cell culture work is finished, the movable door 26 is opened to sterilize and disinfect the inside of the sealed tank body 1, and the cleanliness of the inside of the sealed tank body 1 is ensured.

The above-mentioned embodiment is right the utility model discloses an explanation, it is not right the utility model discloses a limited, any right the scheme after the simple transform of the utility model all belongs to the protection scope of the utility model.

Claims

1. An immune cell culture device, characterized in that: comprises a sealed tank body and a sterilization box which is arranged on one side of the sealed tank body and is used for killing bacteria contained in the air, wherein a first connecting pipe is communicated between the sealed tank body and the sterilization box, the first connecting pipe extends to the upper part of the inner part of the sealed tank body, an exhaust port used for discharging waste gas is arranged on the lower part of one side of the sealed tank body, a second connecting pipe is connected with the exhaust port, a cover body is detachably and fixedly connected with the top of the sealed tank body, a culture tank used for containing cells and culture solution is arranged in the sealed tank body, the culture tank is positioned below the first connecting pipe, symmetrically arranged fixing rods are fixedly connected with the two sides of the outer part of the culture tank, one end of each fixing rod, which deviates from the culture tank, is fixedly connected on the inner wall of the sealed tank body, the top opening of the culture tank is arranged, discharge hole department is connected with downwardly extending's discharging pipe, the discharging pipe runs through the bottom of the sealed tank body and extends to outside the sealed tank body, and the lateral wall of cultivateing the jar is inside hollow bilayer structure, is outer wall and inner wall respectively, spiral winding has the heat source pipe on the inner wall, the upper and lower both ends of heat source pipe all run through the lateral wall of the outer wall and the sealed tank body and extend to the outside of the sealed tank body, the top of lid is equipped with the bellows, the bellows is inside hollow structure, and the bottom fixed connection of bellows has the sampling tube to the inside extension of cultivateing the jar, the sampling tube is linked together with the bellows, and one side fixedly connected with of sampling tube has the drain pipe of the downward sloping setting that is linked.

2. The immune cell culture device of claim 1, wherein: the top fixedly connected with mounting bracket of lid, the top fixedly connected with pneumatic cylinder of mounting bracket, the vertical downward setting of output of pneumatic cylinder, the bottom fixedly connected with movable rod of pneumatic cylinder output, the movable rod extends to the inside of culture tank and fixedly connected with is used for the circular disk with cell and culture solution mixing, set up the water hole of crossing that a plurality of even intervals set up on the circular disk.

3. The immune cell culture device of claim 2, wherein: the sterilizing box comprises a box body, an air blower is arranged on one side of the box body, a third connecting pipe is communicated between the air blower and the box body, an air outlet is formed in one side, away from the air blower, of the box body, one end of the first connecting pipe is connected to the air outlet, a plurality of baffle plates which are arranged in a staggered mode are fixedly connected to the inner wall of the box body, the baffle plates are arranged in a downward inclined mode, an ultraviolet lamp tube is arranged between two adjacent baffle plates located on the same side of the inner wall of the box body, and the ultraviolet lamp tube.

4. The immune cell culture device of claim 2, wherein: the end part of one end of the first connecting pipe extending into the interior of the sealing tank body is sealed, and a plurality of vent holes are formed in the first connecting pipe located in the interior of the sealing tank body.

5. The immune cell culture device of claim 2, wherein: one side of the sealed tank body is provided with a beaker filled with sterilized water, and one end of the second connecting pipe, which is far away from the sealed tank body, is positioned at the lower part of the liquid level of the sterilized water.

6. The immune cell culture device of claim 3, wherein: the ultraviolet lamp tube is a bent tube which rotates in a bow shape.

7. The immune cell culture device of any of claims 1-6, wherein: one side of the sealed tank body is hinged with a movable door, and one side of the movable door and the sealed tank body are provided with door lock mechanisms for locking the movable door.

8. The immune cell culture device of any of claims 1-6, wherein: the corrugated pipe is made of elastic rubber.

9. The immune cell culture device of any of claims 2-6, wherein: rubber sealing rings are sleeved at the joint of the sampling tube and the cover body and the joint of the output end of the hydraulic cylinder and the cover body.