CN219930053U - Microorganism culture and detection device - Google Patents
Microorganism culture and detection device Download PDFInfo
- Publication number
- CN219930053U CN219930053U CN202321641783.2U CN202321641783U CN219930053U CN 219930053 U CN219930053 U CN 219930053U CN 202321641783 U CN202321641783 U CN 202321641783U CN 219930053 U CN219930053 U CN 219930053U
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- Prior art keywords
- culture
- tank
- plunger
- overflow
- microorganism
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- 244000005700 microbiome Species 0.000 title claims abstract description 68
- 238000001514 detection method Methods 0.000 title claims abstract description 43
- 238000000034 method Methods 0.000 claims abstract description 8
- 238000007789 sealing Methods 0.000 claims abstract description 7
- 239000011159 matrix material Substances 0.000 claims description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 4
- 239000000741 silica gel Substances 0.000 claims description 4
- 229910002027 silica gel Inorganic materials 0.000 claims description 4
- 229910001220 stainless steel Inorganic materials 0.000 claims description 4
- 239000010935 stainless steel Substances 0.000 claims description 4
- 239000007788 liquid Substances 0.000 description 4
- 230000001651 autotrophic effect Effects 0.000 description 3
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 210000002220 organoid Anatomy 0.000 description 2
- 229910052717 sulfur Inorganic materials 0.000 description 2
- 239000011593 sulfur Substances 0.000 description 2
- 238000012604 3D cell culture Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000010842 industrial wastewater Substances 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000002352 surface water Substances 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Abstract
The utility model relates to the technical field of microorganism culture plates, and provides a microorganism culture and detection device which comprises a bottom plate and a cover plate, wherein a plurality of culture tanks are formed on one side of the bottom plate; the cover plate is detachably fixed on the bottom plate and is used for selectively sealing the culture tank; the bottom plate is provided with a plurality of overflow tanks, each overflow tank is communicated with two culture tanks, each culture tank is communicated with one overflow tank, and the depth of each overflow tank is smaller than that of each culture tank. According to the utility model, by arranging the overflow groove, when microorganisms are detected, part of microorganism culture solution in the culture groove can be transferred into another culture groove communicated with the culture groove, and an additional pipetting tool is not required, so that the operation procedure is simplified, the microorganisms can be prevented from being polluted or damaged, and the detection efficiency of the microorganism culture and detection device is improved.
Description
Technical Field
The utility model relates to the technical field of microorganism culture plates, in particular to a microorganism culture and detection device.
Background
In recent years, the sulfur autotrophic denitrification technology is gradually applied to the treatment fields of urban sewage, industrial wastewater, nitrate-polluted surface water, underground water and drinking water, and stable autotrophic denitrification microorganisms are cultured and screened out, so that the standardization and large-scale production of the sulfur autotrophic denitrification microorganisms are facilitated, and in the culture process, a microorganism culture and detection device is needed.
The prior publication No. CN218521267U discloses a pore plate for 3D cell culture, which reduces the mechanical disturbance of experimenters to culture glue drops during operations such as liquid exchange and treatment reagent addition, so as to minimize the difference caused by interference during operation of organoids cultured in each conventional sample hole on the whole culture substrate, facilitate the subsequent operations such as standardized detection of organoids in the conventional sample hole, and avoid distortion caused by the difference in operation as much as possible.
In the above technical scheme, in order to eliminate the damage of liquid changing or liquid adding operation to cells, an auxiliary small hole is arranged on one side of a conventional sample hole, however, in the culture process of cells, a part of cells are usually extracted from the conventional sample hole by using a liquid transferring tool for detection, and the operation is complicated and can pollute or damage the cells.
Disclosure of Invention
In view of the above, the utility model provides a microorganism culture and detection device, which can conveniently transfer partial microorganism culture to be detected, and improve culture and detection efficiency.
The technical scheme of the utility model is realized as follows: the utility model provides a microorganism culture and detection device, which comprises a bottom plate and a cover plate, wherein,
a plurality of culture tanks are formed on one side of the bottom plate;
the cover plate is detachably fixed on the bottom plate and is used for selectively sealing the culture tank;
the bottom plate is provided with a plurality of overflow tanks, each overflow tank is communicated with two culture tanks, each culture tank is communicated with one overflow tank, and the depth of each overflow tank is smaller than that of each culture tank.
On the basis of the above technical scheme, preferably, a plurality of culture tanks are arranged in a matrix.
Still more preferably, a plurality of the overflow tanks are arranged in a matrix arrangement.
On the basis of the technical scheme, preferably, the cover plate comprises an inner cover and plungers, wherein the plungers are fixedly arranged on one side, close to the bottom plate, of the inner cover and are in sliding connection with the culture tanks, and the number and the positions of the plungers correspond to those of the culture tanks.
Still more preferably, the length of the plunger is not less than the depth of the isopipe.
Still more preferably, the length of the plunger is less than the depth of the culture tank.
Still further preferably, an end of the plunger remote from the inner cap is tapered, and an outer diameter of the end of the plunger remote from the inner cap is smaller than an outer diameter of the end of the plunger near the inner cap.
Still further preferably, the cover plate further includes an outer cover fixedly disposed at a side of the inner cover remote from the plunger.
Still more preferably, the inner cover and the plunger are made of silica gel, and the outer cover is made of stainless steel.
On the basis of the technical scheme, preferably, 96 culture tanks are arranged.
Compared with the prior art, the microorganism culture and detection device has the following beneficial effects:
(1) By arranging the overflow groove, when detecting microorganisms, partial microorganism culture solution in the culture groove can be transferred into another culture groove communicated with the culture groove without using an additional pipetting tool, so that the operation procedure is simplified, and the microorganisms can be prevented from being polluted or damaged, thereby improving the detection efficiency of the microorganism culture and detection device;
(2) The end part of the plunger is arranged in a cone shape, so that the plunger can be conveniently inserted into the culture tank, and the operation convenience of the microorganism culture and detection device is improved;
(3) Through set up the enclosing cover of stainless steel material on the inner cover, can strengthen the structural strength of apron to this microorganism cultivates and detection device's life has been increased.
Drawings
In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the utility model, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a perspective view of a microorganism culture and detection apparatus according to the present utility model;
FIG. 2 is a cross-sectional view of a microorganism culture and detection apparatus according to the present utility model;
FIG. 3 is an enlarged view of the microorganism culture and detection apparatus of the present utility model at A in FIG. 2;
FIG. 4 is a perspective view of an overflow trough in a microorganism culture and detection apparatus according to the utility model;
FIG. 5 is a perspective view of a plunger in a microorganism culture and detection apparatus according to the present utility model.
Wherein: 1. a bottom plate; 101. a culture tank; 102. an overflow trough; 2. a cover plate; 21. an inner cover; 22. a plunger; 23. and an outer cover.
Detailed Description
The following description of the embodiments of the present utility model will clearly and fully describe the technical aspects of the embodiments of the present utility model, and it is apparent that the described embodiments are only some embodiments of the present utility model, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present utility model without making any inventive effort, are intended to fall within the scope of the present utility model.
As shown in FIGS. 1 to 5, a microorganism culture and detection apparatus of the present utility model comprises a base plate 1 and a cover plate 2.
Wherein, the bottom plate 1 is used for carrying a microorganism culture so as to culture and detect the microorganism culture, one side of the bottom plate 1 is provided with a plurality of culture tanks 101 for storing the microorganism culture and culture solution thereof, the bottom plate 1 is provided with a plurality of overflow tanks 102, each overflow tank 102 is communicated with two culture tanks 101, each culture tank 101 is communicated with one overflow tank 102, and the depth of each overflow tank 102 is smaller than that of each culture tank 101; as shown in fig. 4, the overflow tank 102 is connected to the culture tank 101 at the upper and lower sides, so that when culturing microorganisms, the microorganism culture solution can be stored in the culture tank 101 at the upper side, and when the microorganisms need to be detected, part of the microorganism culture solution in the culture tank 101 at the upper side flows into the culture tank 101 at the lower side along the overflow tank 102 by using the inclined bottom plate 1, and the microorganism culture solution is detected in the culture tank 101 at the lower side, and in the detection process, no additional pipetting tool is needed, so that the operation procedure is simplified, the microorganism culture is prevented from being damaged, and the detection efficiency of the microorganism culture and detection device is improved.
Further, in order to automatically add microorganisms into the culture tank 101 during the cultivation of microorganisms, the plurality of culture tanks 101 are arranged in a regular manner, and it is preferable that the plurality of culture tanks 101 are arranged in a matrix.
Furthermore, in order to achieve synchronous transfer of the plurality of microorganism culture solutions, the plurality of overflow tanks 102 should be arranged in a regular manner, and it is preferable that the plurality of overflow tanks 102 be arranged in a matrix manner.
In a preferred embodiment, the culture tanks 101 are preferably provided with 96 culture tanks, and the microorganism culture and detection device is consistent with a culture plate with common specification, so that the microorganism culture and detection device is convenient to be matched with other equipment for microorganism culture and detection.
The cover plate 2 is used for selectively sealing the culture tank 101, not only protecting microorganisms, but also sealing the culture tank 101 so as to provide an anaerobic environment for the microorganisms, and the cover plate 2 is detachably fixed on the bottom plate 1.
As a preferred embodiment, the cover plate 2 may be provided to include an inner cover 21 and plungers 22, the plungers 22 being fixedly provided at a side of the inner cover 21 near the bottom plate 1 and slidably coupled to the culture tanks 101, the number and positions of the plungers 22 corresponding to those of the culture tanks 101; as shown in fig. 1, by the sliding fit of the plunger 22 and the culture tank 101, the sealing of the culture tank 101 can be realized, and the effects of protecting the microorganisms inside and providing anaerobic environment for the microorganisms can be realized.
Further, as shown in FIG. 3, in order to prevent microorganisms from entering one culture tank 101 to another culture tank 101 in communication therewith during cultivation, the length of the plunger 22 should be not less than the depth of the overflow tank 102, in which case independent use of each culture tank 101 is possible, in conformity with the existing culture plate, and when the plunger 22 is moved upward by a certain distance or the plunger 22 is disengaged from the culture tank 101, pipetting of microorganisms is possible; correspondingly, the length of the plunger 22 should be smaller than the depth of the culture tank 101, so that the inner cover 21 can be attached to the bottom plate 1 when the plunger 22 is inserted into the culture tank 101, and impurities are prevented from entering the overflow tank 102.
Further, it is preferable that the end of the plunger 22 far from the inner cap 21 is tapered, so that the outer diameter of the end of the plunger 22 far from the inner cap 21 is smaller than the outer diameter of the end of the plunger 22 near the inner cap 21, and as shown in fig. 5, the plunger 22 can be conveniently inserted into the culture tank 101 by reducing the outer diameter of the end of the plunger 22 inserted into the culture tank 101, thereby improving the operation convenience of the microorganism culture and detection device.
Optionally, an outer cover 23 may be further disposed in the cover 2, such that the outer cover 23 is fixedly disposed on a side of the inner cover 21 away from the plunger 22, and the outer cover 23 is used to protect the inner cover 21; specifically, the inner cover 21 and the plunger 22 may be made of silica gel, and the outer cover 23 may be made of stainless steel, so that the overall structural strength of the cover plate 2 is improved, and the sealing tightness of the cover plate to the culture tank 101 may be improved by using the flexibility of the silica gel.
The application method of the microorganism culture and detection device comprises the following steps:
when culturing microorganisms, firstly, adding a microorganism culture and a culture solution into one of the culture tanks 101 communicated with the overflow tanks 102, and then inserting the plunger 22 into the culture tank 101 to plug the culture tank; when the detection of microorganisms is required, the cover plate 2 is separated from the bottom plate 1, and then part of microorganisms in the culture tank 101 flows into another culture tank 101 communicated with the cover plate by means of the inclined bottom plate 1 through the overflow tank 102, so that the detection of microorganisms in the other culture tank 101 can be performed.
The foregoing description of the preferred embodiments of the utility model is not intended to be limiting, but rather is intended to cover all modifications, equivalents, alternatives, and improvements that fall within the spirit and scope of the utility model.
Claims (10)
1. A microorganism culture and detection device comprises a bottom plate (1) and a cover plate (2), wherein,
a plurality of culture tanks (101) are arranged on one side of the bottom plate (1);
the cover plate (2) is detachably fixed on the bottom plate (1) and is used for selectively sealing the culture tank (101);
the method is characterized in that: a plurality of overflow grooves (102) are formed in the bottom plate (1), each overflow groove (102) is communicated with two culture grooves (101), each culture groove (101) is communicated with one overflow groove (102), and the depth of each overflow groove (102) is smaller than that of each culture groove (101).
2. A microorganism culture and detection apparatus according to claim 1, wherein: the plurality of culture tanks (101) are arranged in a matrix.
3. A microorganism culture and detection apparatus according to claim 2, wherein: the overflow tanks (102) are arranged in a matrix.
4. A microorganism culture and detection apparatus according to any one of claims 1 to 3, wherein: the cover plate (2) comprises an inner cover (21) and plungers (22), wherein the plungers (22) are fixedly arranged on one side, close to the bottom plate (1), of the inner cover (21) and are in sliding connection with the culture grooves (101), and the number and positions of the plungers (22) correspond to those of the culture grooves (101).
5. The microorganism culture and detection apparatus according to claim 4, wherein: the length of the plunger (22) is not less than the depth of the overflow trough (102).
6. The microorganism culture and detection apparatus according to claim 5, wherein: the length of the plunger (22) is smaller than the depth of the culture tank (101).
7. The microorganism culture and detection apparatus according to claim 6, wherein: the end of the plunger (22) away from the inner cover (21) is conical, and the outer diameter of the end of the plunger (22) away from the inner cover (21) is smaller than the outer diameter of the end of the plunger (22) close to the inner cover (21).
8. The microorganism culture and detection apparatus according to claim 7, wherein: the cover plate (2) further comprises an outer cover (23), and the outer cover (23) is fixedly arranged on one side, far away from the plunger (22), of the inner cover (21).
9. The microorganism culture and detection apparatus of claim 8, wherein: the inner cover (21) and the plunger (22) are made of silica gel, and the outer cover (23) is made of stainless steel.
10. A microorganism culture and detection apparatus according to claim 2 or claim 3, wherein: 96 culture tanks (101) are provided.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321641783.2U CN219930053U (en) | 2023-06-26 | 2023-06-26 | Microorganism culture and detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321641783.2U CN219930053U (en) | 2023-06-26 | 2023-06-26 | Microorganism culture and detection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219930053U true CN219930053U (en) | 2023-10-31 |
Family
ID=88487618
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321641783.2U Active CN219930053U (en) | 2023-06-26 | 2023-06-26 | Microorganism culture and detection device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219930053U (en) |
-
2023
- 2023-06-26 CN CN202321641783.2U patent/CN219930053U/en active Active
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