CN101319189B - Apparatus for electrogenesis microorganism high-pass cultivation and sifting motion - Google Patents
Apparatus for electrogenesis microorganism high-pass cultivation and sifting motion Download PDFInfo
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- CN101319189B CN101319189B CN2008101147296A CN200810114729A CN101319189B CN 101319189 B CN101319189 B CN 101319189B CN 2008101147296 A CN2008101147296 A CN 2008101147296A CN 200810114729 A CN200810114729 A CN 200810114729A CN 101319189 B CN101319189 B CN 101319189B
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- carbon felt
- glass tubing
- graphite
- screw socket
- anode
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E60/00—Enabling technologies; Technologies with a potential or indirect contribution to GHG emissions mitigation
- Y02E60/30—Hydrogen technology
- Y02E60/50—Fuel cells
Abstract
The invention relates to a device for culturing and screening electrogenesis microorganism with high throughout, which can improve the culturing and screening efficiency of the electrogenesis microorganism. The technical proposal is as follows: the device is characterized by comprising a water tank (1), plastic baffle plates (2), a carbon felt (3), anode graphite sticks (4), a rubber plug (5), a screw-type glass tube (6), a proton membrane (7), a bakelite valvecock (8), a load (9) and a cathode graphite stick (10), wherein the carbon felt (3) is arranged on the bottom of the water tank (1), a plurality of the plastic baffle plates (2) are arranged on the carbon felt (3), the screw-type glass tube (6) is arranged in the space enclosed by the plastic baffle plates (2) and the side wall of the water tank, the upper opening of the glass tube (6) is sealed by the rubber plug (5), the anode graphite sticks (4) passes through the rubber plug (5) to enter the screw-type glass tube (6), the proton membrane (7) is sealedly fixed on the bottom of the screw-type glass tube (6) by the bakelite valvecock (8), one end of the cathode graphite stick (10) is inserted into the carbon felt (3), while the other end of the cathode graphite stick (10) stretches out of the water tank (1), and the cathode graphite stick (10) stretches out of the water tank (1) to be connected with each anode graphite stick (4) by load.
Description
Technical field
The invention belongs to electrogenesis microorganism culturing and screening plant field, particularly a kind of device that is used for cultivation of electrogenesis microorganism high-pass and screening that can significantly improve the selectivity and the efficient of cultivation.
Background technology
Microbiological fuel cell (Microbial fuel cell, be called for short MFC) be to be biological catalyst with the electrogenesis microorganism, directly the chemical energy of anode fuel is converted into a kind of special fuel cell of electric energy, it is applied in sewage treatment area, collection electrogenesis and sewage purification are one, have very bright and tempting application prospect.
With electron transport is a kind of novel respiratory form of microorganism to anode, up to the present electrogenesis microorganism with this function does not also have effective cultural method, existing culture technique breaks away from microbiological fuel cell electrogenesis condition in vitro culture, owing to lost the pressure of electrogenesis, the microorganism culturing selectivity is not strong, and efficient is very low.
The electrogenesis microorganism is the core of microbiological fuel cell, yet the present electrogenesis microorganism that has obtained purifies and separates has only ten kinds of less thaies, has seriously restricted the further research for anode electricity generation process and electrogenesis mechanism.
Chinese patent about microbiological fuel cell has 15, " a kind of electrochemical method for enrichment of microorganism, the biosensor that is used for analyzing organic substance and biochemical oxygen demand (BOD) " 00809995.2, " a kind of be used for the use waste water of wastewater treatment and the biofuel cell of active sludge " 00810805.6, " microbiological fuel cell " 200410066753.9, " detecting the method and apparatus of toxic substance in the water with microbiological fuel cell " 03807769.8, " microbiological fuel cell of no film and no amboceptor " 03813043.2, " a kind of bio-toilet " 200610038252.9, " preparation method of bactermiorhodopsin proton exchange film of fuel battery " 200510114213.8, " air cathode biological fuel cell for electric generation from organic waste water " 200510010593.0, " biofuel cell " 200480023415.5, " a kind of is the single pond type microbiological cell of fuel with organic waste water " 200510011855.5, " a kind of medium-free microbial fuel cell " 200510079759.4, " a kind of bitubular type microbiological fuel cell 200610113873.9, " bioreactor-direct microbe fuel cell and uses thereof 200510086618.5, " Stackable single cell microbe fuel battery 200610144991.6, " a kind of microbiological fuel cell " 200420090582.9; 1 " a kind of microbiological fuel cell " 200420090582.9 of utility model patent.Up to the present also there is not a patented technology relevant with screening with the cultivation of electrogenesis microorganism high-pass.
Summary of the invention
The purpose of this invention is to provide a kind of device that the electrogenesis microorganism high-pass is cultivated and screened that is used for that can improve electrogenesis microorganism culturing and screening efficiency.
Technical scheme of the present invention is: a kind of device that is used for cultivation of electrogenesis microorganism high-pass and screening is characterized in that comprising tank (1), plastic septum (2), carbon felt (3), graphite anode rod (4), silica gel plug (5), screw socket Glass tubing (6), proton film (7), bakelite cock (8), load (9) and negative electrode graphite rod (10); Wherein, carbon felt (3) is arranged on the bottom of tank (1), on carbon felt (3), be placed with several piece plastic septum (2), in the space that plastic septum (2) and tank sidewall surround, be provided with screw socket Glass tubing (6), the suitable for reading of screw socket Glass tubing (6) sealed by silica gel plug (5), graphite anode rod (4) through-silicon plug (5) enters screw socket Glass tubing (6) inside, proton film (7) by bakelite cock (8) sealing and fixing in screw socket Glass tubing (6) bottom, negative electrode graphite rod (10) one ends insert in the carbon felt (3), the other end stretches out tank (1), and negative electrode graphite rod (10) stretches out tank (1) and links to each other by load (9) with each graphite anode rod (4).
Beneficial effect of the present invention: this device is designed to the anolyte compartment form of test tube, directly under the condition of actual electrogenesis condition, cultivate the electrogenesis microorganism, taked the configuration of integral type negative electrode simultaneously, can guarantee the consistence of negative electrode condition, than prior art, can significantly improve the specific selectivity and the efficient of cultivation.
The invention will be further described below in conjunction with drawings and Examples.
Description of drawings
Accompanying drawing 1 is the device synoptic diagram that the electrogenesis microorganism high-pass is cultivated and screened;
Accompanying drawing 2 is anode monomer synoptic diagram.
Embodiment
Among Fig. 1, the electrogenesis microorganism high-pass is cultivated and the device of screening is made up of tank (1), plastic septum (2), carbon felt (3), graphite anode rod (4), plug (5), screw socket Glass tubing (6), proton film (7), bakelite cock (8), load (9) and negative electrode graphite rod (10).Tank (1) is as cathode compartment, and is shared by a plurality of monomer M FC, keeps the consistence of cathode potential, gets rid of because the output voltage difference that the difference of cathode potential causes; Carbon felt (3) is placed as negative electrode in tank (1) bottom, and by negative electrode graphite rod (10) projected current.Graphite anode rod (4), plug (5), screw socket Glass tubing (6), proton film (7) and bakelite cock (8) constitute independently MFC anode, proton film (7) by bakelite cock (8) sealing and fixing in screw socket Glass tubing (6) bottom, prevent to leak, can add filler increase microorganic adhesion area in the pipe and reduce polarization, graphite anode rod (4) is drawn the anodic electronics, connects load (9) between anode and the negative electrode.Plug (5) is used to seal anode and keeps anaerobic state.Separate with plastic septum (2) between each anode, prevent contact short circuit each other.For the stopping property of assurance device,, proton film (6) is pressed in the mouth of pipe closely by foraminous bakelite cock (7) in the middle of using.
Tubular type MFC assembling and inoculation need be finished under super clean bench and nitrogen protection.Under protection of nitrogen gas, electrogenesis microbial inoculum to be cultivated is seeded to monomer M FC anode, to keep the activity of anerobe, inoculation is finished and is made the sealing of apparatus plug graphite rod to keep aseptic and anaerobic condition.
Between each monomer M FC anode and integral type negative electrode, connect load (9) and form external circuit, the magnitude of voltage at voltage acquisition system acquisition load two ends.In general, through about 2 days starting period, inoculation has the monomer output voltage of electrogenesis microorganism can raise the increase of simultaneous cell concentration gradually.The height that microorganism electricity generation ability can be used on anode potential under the same extrernal resistance condition characterizes, and the microorganism anode potential that electricity generation ability is strong is low, otherwise then high; Because the configuration of this device integral type negative electrode has guaranteed the consistence of cathode potential, then the variation of output voltage reflection is exactly the variation of anode potential, for several control groups that move simultaneously, the experimental group that electricity generation ability is strong shows as the output voltage height, it is lower that the corresponding more weak experimental group of electricity generation ability shows as output voltage, the experimental group that output voltage is high will be as next step object of cultivating screening, and the experimental group that output voltage is low will be discarded.
Independently anode of microbial fuel cell is used to inoculate the electrogenesis microorganism, and anolyte compartment's sealing is to guarantee anaerobic state and to get rid of living contaminants that the catholyte in the cathode compartment is a potassium ferricyanide solution.
Among Fig. 2, the anode monomer is made up of graphite anode rod (4), plug (5), screw socket Glass tubing (6), proton film (7) and bakelite cock (8); Screw socket Glass tubing (6), proton film (7) and bakelite cock (8) at first are assembled into the anolyte compartment, wherein seal approach is the key of device: oven dry is cleaned in screw socket Glass tubing (6), proton film (7) and bakelite cock (8), evenly smear seal gum at the combining site of screw socket Glass tubing (6) and proton film (7) then, bakelite cock (8) pressing is also placed at ambient temperature until curable adhesive layer; Go up punching at plug (5), graphite anode rod (4) is inserted form tool plug graphite rod as anode.Sterilization was standby after preparation work was finished.
Carbon felt (3) need carry out clear water and soak, chlorohydric acid pickling and sodium hydroxide alkali cleaning and final rinsed with deionized water are to remove remaining impurities influence in the carbon felt (3), negative electrode graphite rod (10) inserts carbon felt (3) and keeps good electricity contact, the integral type negative electrode places tank, and guarantee to be soaked fully by catholyte, catholyte is to use potassium ferricyanide solution, will note the cathode compartment lucifuge in the whole service process, to prevent possible because the Tripotassium iron hexacyanide decomposes the danger that brings.
Above-described embodiment is a more preferably embodiment of the present invention, and common variation that those skilled in the art carries out in the technical solution of the present invention scope and replacement all should be included in protection scope of the present invention.
Claims (1)
1. one kind is used for the device that the electrogenesis microorganism high-pass is cultivated and screened, and it is characterized in that comprising tank (1), plastic septum (2), carbon felt (3), graphite anode rod (4), silica gel plug (5), screw socket Glass tubing (6), proton film (7), bakelite cock (8), load (9) and negative electrode graphite rod (10); Wherein, carbon felt (3) is arranged on the bottom of tank (1), on carbon felt (3), be placed with several piece plastic septum (2), in the space that plastic septum (2) and tank sidewall surround, be provided with screw socket Glass tubing (6), the suitable for reading of screw socket Glass tubing (6) sealed by silica gel plug (5), graphite anode rod (4) through-silicon plug (5) enters screw socket Glass tubing (6) inside, proton film (7) by bakelite cock (8) sealing and fixing in screw socket Glass tubing (6) bottom, negative electrode graphite rod (10) one ends insert in the carbon felt (3), the other end stretches out tank (1), and negative electrode graphite rod (10) stretches out tank (1) and links to each other by load (9) with each graphite anode rod (4).
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| Application Number | Priority Date | Filing Date | Title |
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| CN2008101147296A CN101319189B (en) | 2008-06-11 | 2008-06-11 | Apparatus for electrogenesis microorganism high-pass cultivation and sifting motion |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2008101147296A CN101319189B (en) | 2008-06-11 | 2008-06-11 | Apparatus for electrogenesis microorganism high-pass cultivation and sifting motion |
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| Publication Number | Publication Date |
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| CN101319189A CN101319189A (en) | 2008-12-10 |
| CN101319189B true CN101319189B (en) | 2011-11-09 |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101748058B (en) * | 2009-12-28 | 2012-06-20 | 厦门大学 | Screening device used for microorganisms with electro catalytic activity |
| CN102586388A (en) * | 2012-03-08 | 2012-07-18 | 东南大学 | Method for screening electricigens |
| CN114250146A (en) * | 2022-01-19 | 2022-03-29 | 中国科技开发院江苏分院 | Device and method for producing acetic acid by reducing carbon dioxide with electrode |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1588683A (en) * | 2004-09-24 | 2005-03-02 | 浙江大学 | Microbial fuel cell |
| CN1937297A (en) * | 2006-10-20 | 2007-03-28 | 清华大学 | Double-drum microbial fuel cell |
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- 2008-06-11 CN CN2008101147296A patent/CN101319189B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1588683A (en) * | 2004-09-24 | 2005-03-02 | 浙江大学 | Microbial fuel cell |
| CN1937297A (en) * | 2006-10-20 | 2007-03-28 | 清华大学 | Double-drum microbial fuel cell |
Non-Patent Citations (1)
| Title |
|---|
| 李登兰 等.微生物燃料电池构造研究进展.《应用与环境生物学报》.2008,第14卷(第1期),147-152. * |
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