CN217111713U - Indoor simulation culture column for sediment-water interface - Google Patents

Abstract

A sediment-water interface indoor simulation culture column relates to marine ecological experimental equipment. The sample collecting device is provided with an acrylic column body, a PE support bottom, a PE upper cover, a metal supporting strip and a sample collecting port; the cylinder body is connected with the support bottom in a sealing way through acrylic adhesive, the support bottom and the upper cover are correspondingly provided with threaded through holes, and the metal support bar penetrates through the threaded through holes to be connected with the support bottom and the upper cover and is locked through the upper cover; the sample collecting ports are uniformly distributed at the lower part of the cylinder and used for sampling, and the sample collecting ports are sealed by small silica gel plugs; at least two top openings are formed in the middle of the upper cover and used for filling nitrogen into water in the column during an experiment, and the top openings are sealed or opened by large silica gel plugs. The experiment can be simulated in the corresponding water-sediment environment in the cylinder, the leakproofness is good, simple structure is reasonable, can stably keep inside gas tightness in the experimentation, can carry out the cultivation experiment of hypoxemia/anaerobism to have the stopper mouth of convenient sample and detection, provide a feasible environmental simulation and cultivate the system.

Description

Indoor simulation culture column for sediment-water interface

Technical Field

The utility model relates to an ecological experimental facilities in ocean specifically relates to a post is cultivateed in indoor simulation of deposit-water interface.

Background

In the research of environmental ecology, the simulated culture system under laboratory conditions can help researchers to accurately know the process mechanism difference under the biochemical physical state in the environment, and is an effective research means for knowing the environmental ecology.

Compared with the traditional pure culture system, the method can simulate some special complex systems in the natural environment on the basis of the natural microbial community; in addition, compared with sampling in the field environment, the method has the characteristics of controllable conditions, convenience in sampling, maximum approach to the field environment and the like, such as anoxic water bodies, sediment-water interfaces, water body acidification, temperature rise and the like, so that scientific researchers can further know the scientific problems in the biogeography process. However, under the problem of ocean hypoxia which is becoming too large, the establishment of related laboratory simulation culture systems is less, and simulation systems which achieve good air tightness and can perform sample collection and continuous hypoxia/hypoxia culture are not established.

Disclosure of Invention

The utility model aims to overcome the defects of the experimental condition limitation and the prior art, and provides a sediment-water interface indoor simulation culture column which is easy to obtain materials, simple and reasonable in structure and convenient to sample and has good air tightness.

The utility model is provided with an acrylic column body, a PE support bottom, a PE upper cover, a metal support strip and a sample collection port; the acrylic column body is connected with the PE support bottom in a sealing mode through acrylic glue, a plurality of threaded through holes are correspondingly formed in the PE support bottom and the PE upper cover, threads are formed in the upper end and the lower end of the metal supporting strip, the metal supporting strip penetrates through the threaded through holes to be connected with the PE support bottom and the PE upper cover and is locked through the upper cover wing screw cap and the support bottom threaded hole; the sample collecting ports are uniformly distributed at the lower part of the acrylic cylinder and used for sampling, and the sample collecting ports are sealed by small silica gel plugs; the middle part of the PE upper cover is provided with at least two top openings for filling nitrogen into water in the column during experiments, and the top openings are sealed or opened by large silica gel plugs.

The upper surface of the PE support base and the lower surface of the PE upper cover are respectively provided with a circular groove, the acrylic column is embedded into the circular grooves, and a silica gel pad can be arranged in the circular grooves in a cushioning mode and used for sealing and shock prevention.

The number of the sample collecting ports is at least 3, the maximum number of the sample collecting ports is 6 under the condition of ensuring the structural stability and the sealing performance of the column body, the interval between every two collecting ports is 5cm in the vertical direction, the interval is 5cm around the circumference of the column body longitudinally, and the sample collecting ports comprise PE threaded connecting pipes, silica gel pads, screw caps and small silica gel plugs; the lower part of the column body is provided with a round opening, the round opening is physically polished to be smooth, spiral threads are arranged on the outer side of the PE threaded connecting pipe, the PE threaded connecting pipe is inserted into the round opening to be communicated with the inside and the outside of the column body, the inner side and the outer side of the PE communicating pipe are fixed through a silica gel pad and a spiral cap, and the pipe opening of the PE threaded connecting pipe is sealed by a small silica gel plug.

At least 3 metal supporting bars are arranged.

When the device is used, the upper cover can be detached through the screw caps on the upper parts of the four metal strips through which the upper cover penetrates, so that the column body is opened, and an environment simulation culture system, such as sediment and seawater, can be added into the column body under the condition that the sampling hole below is ensured to be closed. After the addition is finished, the upper cover is installed and the four screw caps are screwed. After all the sealed cells are sealed, corresponding culture experiments can be developed.

Compared with the prior art, the utility model discloses mainly have following outstanding advantage and technological effect:

1. the utility model discloses can open corresponding water-deposit environmental simulation experiment in the cylinder, through the sealed of upper cover, can keep the isolated of gas in the post and outside gas, reach the sealed effect in the post, guarantee the development of low oxygen, oxygen deficiency experiment and the collection of sample.

2. The PE support base can support the whole column to stably stand on a flat ground and provides the shock-proof and buffering capacity of the base.

3. The upper part of the column can be sealed by a PE upper cover, and the capabilities of shock resistance, buffering, gas filling/discharging, upper layer sample extraction and additives are provided.

4. The stainless steel metal support bars can improve the stability of the whole cylinder and between the support bottom and the upper cover, and ensure the sealing property between the upper cover and the cylinder.

5. The sample collection port is arranged to facilitate acquisition of samples of bottom water and sediments, and simultaneously, gas sealing of the lower sampling port, stability of the opening and feasibility of sample acquisition are guaranteed.

6. The utility model adopts the acrylic component and the PE component with light weight, and realizes the sealing in the column through the silica gel plug and the silica gel pad; thereby reducing the self weight and ensuring the air tightness of the sample culture and the convenience in sampling.

6. The utility model discloses the leakproofness is good, and simple structure is reasonable, can stably keep inside gas tightness in the experimentation, can carry out the cultivation experiment of hypoxemia/anaerobism to have the stopper mouth of convenient sample and detection, provide a feasible environmental simulation and cultivate the system.

Drawings

Fig. 1 is a schematic view of the structure of the embodiment of the present invention.

Fig. 2 is a schematic side view of the embodiment of the present invention.

Fig. 3 is a schematic top view of the embodiment of the present invention.

Fig. 4 is a schematic bottom view of the embodiment of the present invention.

Fig. 5 is a schematic structural diagram of the PE upper cover according to an embodiment of the present invention.

Fig. 6 is a schematic structural view of a sample collection port according to an embodiment of the present invention.

Each of FIGS. 1-6 is labeled as: 1. a large silica gel plug; 2. a screw cap with wings; 3. covering the PE; 4. a metal support bar; 5. a cylinder; 6. a sample collection port; 7. supporting the PE bottom; 8. a shockproof pad; 9. a silica gel ring; 10. a small silica gel plug; 11. a PE thread connecting pipe; 12. a communicating port screw cap; 13. a silica gel pad.

FIG. 7 is a line graph showing the change of dissolved oxygen during the culture in the water columns No. 1 and No. 2 of the hypoxic culture experiment.

Detailed Description

The following examples will further illustrate the present invention with reference to the accompanying drawings.

As shown in fig. 1-6, the embodiment of the present invention is provided with an integrated acrylic organic glass body wall and an acrylic bottom connected by acrylic glue to form an acrylic cylinder 5, and a PE support bottom 7 and a PE upper cover 3 are further provided above and below the acrylic cylinder 5; a plurality of threaded through holes are correspondingly formed in the PE support base 7 and the PE upper cover 3, and 4 stainless steel metal strips are arranged between the PE support base 7 and the PE upper cover 3 and serve as a support stable structure; the upper end and the lower end of the metal support strip 4 are both provided with threads, the metal support strip 4 penetrates through a thread through hole to be connected with the PE support bottom 7 and the PE upper cover 3 and is locked by the upper cover wing screw cap 2 and the support bottom threaded hole; the column body is provided with 3 physical polishing openings, and the physical polishing openings are used as sample collecting openings through a silica gel pad, a PE communicating pipe, a silica gel plug and a communicating opening screw cap; the sample collecting ports 6 are uniformly distributed at the lower part of the acrylic cylinder 5 and used for sampling, and the sample collecting ports 6 are sealed by small silica gel plugs 10; at least two top openings are formed in the middle of the PE upper cover 3 and used for filling nitrogen into water in the column during an experiment, and the top openings are sealed or opened by large silica gel plugs 1. Corresponding water-sediment environment simulation experiments can be performed in the column, the isolation of gas in the column and external gas can be kept through the sealing of the upper cover, the sealing effect in the column is achieved, and the development of low-oxygen and oxygen-poor experiments and the collection of samples are guaranteed.

The PE support is provided with holes around the bottom for penetrating through an external stainless steel metal support strip, one side of the PE support facing upwards is provided with a circle of groove embedded with the wall of an acrylic body, a silica gel ring 9 with corresponding size is arranged in the groove for sealing and shock prevention, and a sponge gasket is attached to the side facing downwards. The PE support bottom is used for supporting the whole column to stably stand on a flat ground and providing the shock-proof and buffering capacity of the bottom.

Holes are punched around the PE upper cover to penetrate through an external stainless steel metal supporting strip, a circle of groove which is embedded with the wall of the acrylic body is arranged on the downward surface of the middle part of the PE upper cover, and a silica gel ring 9 with a corresponding size is arranged in the groove and used for sealing and shock prevention; in addition, the middle of the upper cover is provided with two openings, and a silica gel plug with corresponding size is arranged. The PE upper cap is used to seal the upper part of the column and provide shock resistance, buffering, gas filling/discharging, upper sample extraction and additive capabilities.

4 stainless steel metal support bars, the upper and lower parts of which are provided with screw threads, pass through corresponding holes of the PE support bottom and the PE upper cover and are fixed by nuts; the metal support strip is used for improving the stability between the whole column body and the PE support bottom and the PE upper cover and ensuring the sealing property between the upper cover and the column body.

The sample collection port 6 is located on the circular opening of the cylinder and is composed of a silica gel pad, a PE threaded connection pipe, a small silica gel plug and a screw cap on two sides, so that the gas sealing of the lower sampling port, the stability of the opening and the feasibility of sample acquisition are guaranteed. Sample collection mouth 6 is equipped with 3 at least, set up 6 collection mouths at most under the condition of guaranteeing cylinder structural stability and leakproofness, every collects 5cm in vertical direction interval between the mouth 6, vertically around 5 girth intervals 5cm of ya keli cylinder, it is smooth to set up round mouth and physics in 5 lower parts of yakeli cylinder, the PE screwed connection outside of tubes has the spiral line, PE screwed connection inserts inside and outside the round mouth intercommunication cylinder, the silica gel pad is passed through to the inside and outside both sides of PE intercommunication pipe, the screw cap is fixed, PE screwed connection's mouth of pipe is sealed with little silica gel plug.

Specific examples are given below.

The embodiment comprises a main cylinder and other parts, the lower part of the acrylic cylinder 5 is provided with 3 smooth holes, the bottom of the acrylic cylinder 5 is connected with an acrylic flat bottom through acrylic glue in an embedded mode, the thickness of the acrylic flat bottom is 0.9cm, the embedded depth is 0.3cm, the upper part of the acrylic flat bottom is not sealed, the whole acrylic cylinder 5 is barrel-shaped, the height is 70cm, the width is 15cm, and the volume is about 12.4L.

The lower part of the acrylic column 5 is embedded into a groove on the upward surface of the PE support bottom 7, the groove is 0.4cm deep and 1cm wide, a silica gel ring 9 is arranged in the groove to serve as a buffer, and the diameter of the silica gel ring 9 can be 16cm and the thickness of the silica gel ring can be 1 cm; the downward surface of the PE support bottom 7 is adhered with a shockproof gasket 8, the periphery of the PE support bottom 7 is provided with 4 through holes, and the hole diameter of the hole of the PE support bottom 7 is 0.85cm and the depth is 2 cm.

An opening at the upper part of the acrylic column 5 is embedded into a groove on the downward surface of the PE upper cover 3, the groove structure is consistent with that of a groove of the PE support bottom 7, a silica gel ring 9 is also arranged in the groove to play the roles of sealing and shock resistance, two openings with the diameter of 1.5cm are formed in the middle part of the PE upper cover 3 and are sealed by a large silica gel plug 1, the large silica gel plug is in a circular table shape, the maximum outer diameter is 1.8cm, and the vertical height is 2.6 cm; through holes with the diameter of 0.85cm are formed around the PE upper cover 3; the PE support bottom 7 and the PE upper cover 3 are connected through 4 metal support strips 4 made of 315 stainless steel, the length of each metal support strip 4 is 75cm, the upper portion and the lower portion of each metal support strip 4 are provided with threads, each metal support strip 4 penetrates through the corresponding through hole in the PE support bottom 7 and the corresponding through hole in the PE upper cover 3, a nut is added for fixing the stainless steel support strips, the upper portions of the metal support strips protrude out of the openings of the upper cover by 2cm, the winged screw caps 2 are used for screwing, and the PE upper cover can be conveniently detached at any time to be opened.

The inferior part of inferior gram force cylinder 5 has 3 smooth openings of polishing of physics to as sample collection mouth 6 after assembling through silica gel pad 13, PE threaded connection pipe 11, little silica gel stopper 10 and intercommunication mouth screw cap 12, be fixed in the cylinder opening part through screwing intercommunication mouth screw cap 12 and PE threaded connection pipe 11, and sealed with little silica gel stopper 10, the difference in height is 5cm between every trompil. The PEPE threaded connecting pipe 11 is 4cm in length, and the diameter of the nut is 3 cm; the small silica gel plug is in a round table shape, the maximum outer diameter is 1cm, and the vertical height is 2.6 cm; the inner diameter of the communicating port spiral cap 12 is 1.9cm, the outer diameter is 3cm, and the thickness is 0.6 cm; the silica gel pad 13 had an inner diameter of 1.9cm, an outer diameter of 3cm and a thickness of 0.3 cm. The sample collection port 6 is used for sample acquisition of the bottom water and sediment.

The utility model discloses a use method refers as follows:

through the utility model discloses carry out the hypoxemia and cultivate: taking off the upper cover wing screw cap, opening the upper cover, adding sediment and culturing the required seawater in the column, covering the upper cover again and buckling the upper cover, screwing the wing screw cap, filling nitrogen into the water body in the column through the opening in the middle of the upper cover, removing oxygen in the water body after a period of time, then plugging the opening by using a silica gel plug, performing sealed culture, and collecting the sample according to experimental design.

FIG. 7 shows the line graphs of the change of dissolved oxygen during the culture of the experiment No. 1 water column and No. 2 water column. After the sediment-water sample is cultured for 131 days by using the utility model, the lower layer in the cylinder body is marine sediment, the upper layer is in-situ seawater, and the headspace is nitrogen; as can be seen from FIG. 7, the change of dissolved oxygen in the bottom water in the sediment-water interface indoor simulation culture column is stabilized below 0.2mg/L and reaches the threshold of anaerobic metabolism of microorganisms from 20 days to 131 days of low-oxygen culture (the dissolved oxygen in the common water is greater than 7mg/L), and experiment 1 and experiment 2 in FIG. 7 are two water column samples for culture respectively. This experiment purpose is verified the utility model discloses an leakproofness and feasibility.

Through experimental verification, can stably keep inside gas tightness during closed cultivation, can carry out the cultivation experiment of hypoxemia/anaerobism to have the stopper mouth of convenient sample and detection, the utility model provides a feasible environmental simulation cultivates system.

Claims (6)

1. A sediment-water interface indoor simulation culture column is characterized by being provided with an acrylic column body, a PE support bottom, a PE upper cover, a metal support strip and a sample collection port; the acrylic column body is connected with the PE support bottom in a sealing mode through acrylic glue, a plurality of threaded through holes are correspondingly formed in the PE support bottom and the PE upper cover, threads are formed in the upper end and the lower end of the metal supporting strip, the metal supporting strip penetrates through the threaded through holes to be connected with the PE support bottom and the PE upper cover and is locked through the upper cover wing screw cap and the support bottom threaded hole; the sample collecting ports are uniformly distributed at the lower part of the acrylic cylinder and used for sampling, and the sample collecting ports are sealed by small silica gel plugs; and the middle part of the PE upper cover is provided with at least two top openings for filling nitrogen into the water body in the column during the experiment, and the top openings are sealed or opened by large silica gel plugs.

2. The simulation culture column for a sediment-water interface chamber as claimed in claim 1, wherein the upper surface of the PE tray and the lower surface of the PE upper cover are provided with circular grooves, the acrylic columns are embedded in the circular grooves, and the silicon rubber pads are arranged in the circular grooves for sealing and shock-proof.

3. The sediment-water interface chamber simulation culture column of claim 1, wherein the number of the sample collection ports is at least 3, and the number of the sample collection ports is at most 6 under the condition of ensuring the structural stability and the sealing performance of the column body.

4. The sediment-water interface chamber simulation culture column of claim 3, wherein each sample collection port is vertically spaced 5cm apart and longitudinally spaced 5cm apart around the perimeter of the column.

5. The sediment-water interface chamber simulation culture column of claim 1, wherein the sample collection port comprises a PE threaded connection tube, a silica gel pad, a screw cap, a small silica gel plug; the lower part of the column body is provided with a round opening, the round opening is physically polished to be smooth, spiral threads are arranged on the outer side of the PE threaded connecting pipe, the PE threaded connecting pipe is inserted into the round opening to be communicated with the inside and the outside of the column body, the inner side and the outer side of the PE communicating pipe are fixed through a silica gel pad and a spiral cap, and the pipe opening of the PE threaded connecting pipe is sealed by a small silica gel plug.

6. The sediment-water interface chamber simulation culture column of claim 1, wherein the metal support strip is provided with at least 3.

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