CN110673324A - Glass slide structure suitable for confocal microscope - Google Patents
Glass slide structure suitable for confocal microscope Download PDFInfo
- Publication number
- CN110673324A CN110673324A CN201910921584.9A CN201910921584A CN110673324A CN 110673324 A CN110673324 A CN 110673324A CN 201910921584 A CN201910921584 A CN 201910921584A CN 110673324 A CN110673324 A CN 110673324A
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- China
- Prior art keywords
- glass
- slide
- glass slide
- cover glass
- sealing ring
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Links
- 239000011521 glass Substances 0.000 title claims abstract description 93
- 239000006059 cover glass Substances 0.000 claims abstract description 53
- 238000007789 sealing Methods 0.000 claims abstract description 39
- 239000013013 elastic material Substances 0.000 claims abstract description 4
- 238000004624 confocal microscopy Methods 0.000 claims 2
- 238000000034 method Methods 0.000 claims 1
- 230000005574 cross-species transmission Effects 0.000 abstract description 2
- BFMYDTVEBKDAKJ-UHFFFAOYSA-L disodium;(2',7'-dibromo-3',6'-dioxido-3-oxospiro[2-benzofuran-1,9'-xanthene]-4'-yl)mercury;hydrate Chemical compound O.[Na+].[Na+].O1C(=O)C2=CC=CC=C2C21C1=CC(Br)=C([O-])C([Hg])=C1OC1=C2C=C(Br)C([O-])=C1 BFMYDTVEBKDAKJ-UHFFFAOYSA-L 0.000 abstract description 2
- 239000003795 chemical substances by application Substances 0.000 description 13
- 239000007850 fluorescent dye Substances 0.000 description 13
- 230000000694 effects Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- FWBHETKCLVMNFS-UHFFFAOYSA-N 4',6-Diamino-2-phenylindol Chemical compound C1=CC(C(=N)N)=CC=C1C1=CC2=CC=C(C(N)=N)C=C2N1 FWBHETKCLVMNFS-UHFFFAOYSA-N 0.000 description 1
- 239000004831 Hot glue Substances 0.000 description 1
- 239000003364 biologic glue Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- OSWPMRLSEDHDFF-UHFFFAOYSA-N methyl salicylate Chemical compound COC(=O)C1=CC=CC=C1O OSWPMRLSEDHDFF-UHFFFAOYSA-N 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 239000009637 wintergreen oil Substances 0.000 description 1
Images
Classifications
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
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- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Microscoopes, Condenser (AREA)
Abstract
The invention discloses a glass slide structure suitable for a confocal microscope, which comprises: the middle glass slide is provided with a plurality of first through holes; the cover glass, the intermediate glass and the glass slide are sequentially stacked; and the sealing ring is made of elastic material, is arranged in the first through hole, and has a thickness slightly larger than that of the intermediate glass slide, so that the upper side and the lower side of the sealing ring are respectively abutted against the cover glass and the glass slide when the cover glass, the intermediate glass slide and the glass slide are sequentially attached. Through setting up the sealing washer, put middle slide on the glass slide earlier during the use, then put the sealing washer in first through-hole, then arrange the observation thing in the sealing washer, then cover the glass slide, use external clamping piece clamp cover glass, middle slide and glass slide again, because the upper and lower side of sealing washer respectively with the cover glass slide butt, the sanitizer and fluorochrome are difficult to spill over.
Description
Technical Field
The invention relates to the field of experimental equipment, in particular to a glass slide structure suitable for a confocal microscope.
Background
The laser confocal microscope used in many laboratories in China at present is of an inverted type, and generally, a mixture of an observed object, a transparent agent and a fluorescent dye is placed above an objective lens in two ways; one of the modes is to use a confocal special culture dish, a glass sheet with the thickness of about 0.17mm is embedded at the bottom of a plastic culture dish body, the glass sheet is bonded with the bottom of the culture dish body through a special transparent biological adhesive, and the confocal special culture dish is directly placed above an objective lens, but the confocal special culture dish is difficult to clean and sterilize and can only be used once, so that the experiment cost is high; the other mode is that a conventional combined structure of a cover glass and a glass slide is used, an observed object is placed on the glass slide, a transparent agent and a fluorescent dye are dripped, then the glass slide is covered, then the whole combined structure of the cover glass and the glass slide is turned over and then placed above an objective lens, due to the surface tension effect of liquid, the cover glass and the glass slide can be kept attached and cannot fall off, but the space between the glass slide and the glass slide is small, the transparent agent and the fluorescent dye are easy to overflow, most of the transparent agent (such as wintergreen oil) and the fluorescent Dye (DAPI) used in an experiment have pungent smell and toxicity, and the common contact can cause harm to a human body.
Disclosure of Invention
The present invention is directed to solving at least one of the problems of the prior art. Therefore, the invention provides a glass slide structure suitable for a confocal microscope, and a transparent agent and a fluorescent dye are not easy to overflow when the glass slide structure is used.
A slide structure suitable for a confocal microscope according to an embodiment of the present invention includes: the middle glass slide is provided with a plurality of first through holes; the cover glass, the intermediate glass and the glass slide are sequentially stacked; and the sealing ring is made of elastic material, is arranged in the first through hole, and has a thickness slightly larger than that of the intermediate glass slide, so that the upper side and the lower side of the sealing ring are respectively abutted against the cover glass and the glass slide when the cover glass, the intermediate glass slide and the glass slide are sequentially attached.
The slide structure suitable for the confocal microscope according to the embodiment of the invention has at least the following technical effects: through setting up the sealing washer, put middle slide on the glass slide earlier during the use, then put the sealing washer in first through-hole, then arrange the observation thing in the sealing washer, then cover the glass slide, use external clamping piece clamp cover glass, middle slide and glass slide again, because the upper and lower side of sealing washer respectively with the cover glass slide butt, the sanitizer and fluorochrome are difficult to spill over.
According to some embodiments of the invention, a first gap is provided between an inner side wall of the first through hole and the seal ring.
According to some embodiments of the invention, the seal ring is black in color.
According to some embodiments of the invention, the first through hole is two.
According to some embodiments of the invention, the cover glass, the intermediate glass and the glass slide are arranged between the upper clamping part and the lower clamping part, the upper clamping part is abutted with the cover glass, and the lower clamping part is abutted with the glass slide, so that the cover glass, the intermediate glass and the glass slide are sequentially attached.
According to some embodiments of the invention, the cover glass is in a shape of a transversely arranged rectangular plate, and the number of the clamping members is two, and the two clamping members are respectively arranged at the left end and the right end of the cover glass.
According to some embodiments of the invention, the clamp is a clip.
Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.
Drawings
The above and/or additional aspects and advantages of the present invention will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings of which:
FIG. 1 is a front elevational view of a slide assembly in accordance with an embodiment of the present invention;
FIG. 2 is a top view of a configuration of a slide assembly of an embodiment of the invention;
figure 3 is a schematic view of the assembled slide assembly and clamping member shown in figure 2.
Reference numerals:
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the accompanying drawings are illustrative only for the purpose of explaining the present invention, and are not to be construed as limiting the present invention.
In the description of the present invention, it should be understood that the orientation or positional relationship referred to in the description of the orientation, such as the upper, lower, front, rear, left, right, etc., is based on the orientation or positional relationship shown in the drawings, and is only for convenience of description and simplification of description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. The terms "a" or "an" mean one or more, the terms "a" or "an" mean two or more, the terms larger than, smaller than, exceeding, and the like are understood to include the number, and the terms "larger than, smaller than, within, and the like are understood to include the number. If the first and second are described for the purpose of distinguishing technical features, they are not to be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated or implicitly indicating the precedence of the technical features indicated.
In the description of the present invention, unless otherwise explicitly limited, terms such as arrangement, installation, connection and the like should be understood in a broad sense, and those skilled in the art can reasonably determine the specific meanings of the above terms in the present invention in combination with the specific contents of the technical solutions.
A slide structure suitable for a confocal microscope according to an embodiment of the present invention is described below with reference to fig. 1 and 2.
As shown in fig. 1 and 2, a slide structure suitable for a confocal microscope according to an embodiment of the present invention includes a cover glass 110, an intermediate slide 120, a slide 130, and a sealing ring 140.
The middle slide 120 is provided with a plurality of first through holes; the cover glass 110, the intermediate glass 120 and the glass slide 130 are sequentially stacked; the sealing ring 140 is made of an elastic material, and the sealing ring 140 is disposed in the first through hole and has a thickness slightly larger than that of the intermediate slide 120, so that the upper and lower sides of the sealing ring 140 abut against the cover glass 110 and the slide 130 when the cover glass 110, the intermediate slide 120 and the slide 130 are sequentially attached.
For example, as shown in fig. 1 and 2, the cover glass 110, the intermediate glass 120, the glass slide 130, and the sealing ring 140 are collectively referred to as a glass slide assembly 100, the cover glass 110, the intermediate glass 120, and the glass slide 130 may be rectangular plates arranged in a transverse direction, the cover glass 110, the intermediate glass 120, and the glass slide 130 are sequentially stacked from top to bottom, and the sealing ring 140 is disposed between the cover glass 110 and the glass slide 130 and within the first through hole.
When the device is used, the middle glass slide 120 is placed on the glass slide 130, the sealing ring 140 is placed in the first through hole, the mixture of the observed object, the transparent agent and the fluorescent dye is placed in the sealing ring 140, the cover glass 110 is covered, the cover glass 110, the middle glass slide 120 and the glass slide 130 are clamped by the external clamping piece 200, the whole body can be turned over and placed above the objective lens, the transparent agent and the fluorescent dye in the observed object are not easy to overflow due to the fact that the upper side and the lower side of the sealing ring 140 are respectively abutted to the cover glass 110 and the glass slide 130, and the sealing ring 140 can be attached to the glass slide 130 through hot melt adhesive, so that the transparent agent and the fluorescent dye cannot flow out from the space between the sealing ring 140 and the glass slide 130 when; meanwhile, after the use, the cover glass 110, the intermediate glass 120, the glass slide 130 and the sealing ring 140 can be disassembled for cleaning and sterilization respectively, so that the device can be repeatedly used, and the experiment cost is low.
In addition, the cover glass 110, the glass slide 130 and the sealing ring 140 can be purchased directly, and the intermediate glass 120 can be formed by opening the other glass slide 130 by using a glass opener, so that the structure is simple, the manufacturing is simple and convenient, and the popularization and the use are convenient.
In some embodiments of the present invention, a first gap is provided between an inner sidewall of the first through hole and the sealing ring 140. The diameter of the first through hole can be made larger than the outer diameter of the sealing ring 140 when the intermediate glass sheet 120 is processed, so that a first gap exists between the sealing ring 140 and the inner sidewall of the first through hole in the first through hole, but the first through hole can also be processed on the intermediate glass sheet 120, and then the sealing ring 140 with the outer diameter smaller than the diameter of the first through hole is purchased. Thus, when the observation object is placed in the sealing ring 140, the amount of the transparent agent and the fluorescent dye can be slightly increased to slightly overflow the sealing ring 140, then the cover glass 110 is covered, the air in the sealing ring 140 is discharged, the interference of the air is removed, the overflowing transparent agent and the overflowing fluorescent dye can be remained in the first gap, the transparent agent and the fluorescent dye cannot be extruded when the glass slide 130, the middle glass sheet 120 and the cover glass 110 are tightly attached, and the transparent agent and the fluorescent dye cannot be contacted when an experimenter turns over and moves the invention.
According to some embodiments of the present invention, the color of the sealing ring 140 is black. When there are more than two first through holes, the black sealing ring 140 can avoid the influence of fluorescence between the holes when the confocal microscope takes a picture.
According to some embodiments of the invention, the first through hole is two. As shown in fig. 1 and 2, the intermediate slide 120 has two first through holes, the slide 130, the intermediate slide 120 and the cover glass 110 are in a shape of a horizontally arranged rectangle plate, and the two first through holes are arranged at left and right intervals, so that two samples can be observed at one time, and the experiment operation is simplified. It should be noted that the number of the first through holes is at least one, and when there is one, the first through holes are not shown in the drawings, and the first through holes may be provided in the middle of the intermediate glass sheet 120.
According to some embodiments of the present invention, the present invention further comprises a plurality of clamping members 200, the clamping members 200 having an upper clamping portion and a lower clamping portion, the cover glass 110, the intermediate glass 120 and the glass slide 130 being disposed between the upper clamping portion and the lower clamping portion, the upper clamping portion abutting the cover glass 110 and the lower clamping portion abutting the glass slide 130, such that the cover glass 110, the intermediate glass 120 and the glass slide 130 are sequentially abutted. Referring to fig. 3, the cover glass 110 is in a shape of a horizontally arranged rectangular plate, two clamping members 200 are provided, the two clamping members 200 are respectively provided at the left end and the right end of the cover glass 110 to clamp the cover glass 110, the intermediate glass 120 and the glass slide 130, so that the sealing ring 140 is kept in contact with the cover glass 110 and the glass slide 130, and the transparent agent and the fluorescent dye are not overflowed even if the cover glass 110 is turned over and the present invention is moved; and the two clamping members 200 are respectively provided at the left and right ends of the cover glass 110 to make the present invention more robust. It should be noted that the number of the clamping members 200 is at least one, and when one, the clamping member 200 is not shown in the drawings, and the clamping member 200 can be disposed between the two first through holes, and the purpose of clamping the cover glass 110, the intermediate glass 120 and the glass slide 130 can be achieved.
According to some embodiments of the invention, the clamp 200 is a paperclip. The clip has low cost, can be directly obtained by purchasing, and is simple and convenient to use; moreover, the clip is flat and has small volume, and the thickness and the volume of the whole slide structure are less influenced by clamping the cover glass 110, the intermediate glass 120 and the glass slide 130 by the clip, so that the slide structure can be flexibly arranged at a required position.
While the preferred embodiments of the present invention have been illustrated and described, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined in the appended claims.
Claims (7)
1. A glass slide structure suitable for a confocal microscope is characterized in that: the method comprises the following steps:
the middle glass sheet is provided with a plurality of first through holes;
the cover glass, the intermediate glass and the glass slide are sequentially stacked;
and the sealing ring is made of elastic material, is arranged in the first through hole, and has a thickness slightly larger than that of the intermediate glass slide, so that the upper side and the lower side of the sealing ring are respectively abutted against the cover glass and the glass slide when the cover glass, the intermediate glass slide and the glass slide are sequentially attached.
2. A slide structure suitable for use in a confocal microscope according to claim 1, wherein: a first gap is arranged between the inner side wall of the first through hole and the sealing ring.
3. A slide structure suitable for use in a confocal microscope according to claim 1, wherein: the color of the sealing ring is black.
4. A slide structure suitable for use in a confocal microscope according to claim 1, wherein: the number of the first through holes is two.
5. A slide structure suitable for use in a confocal microscope according to claim 1, wherein: the clamping piece is provided with an upper clamping part and a lower clamping part, the cover glass, the middle glass sheet and the glass slide are arranged between the upper clamping part and the lower clamping part, the upper clamping part is abutted against the cover glass, and the lower clamping part is abutted against the glass slide, so that the cover glass, the middle glass sheet and the glass slide are sequentially attached to one another.
6. The slide structure for confocal microscopy according to claim 5, wherein: the cover glass is in a transversely arranged rectangular plate shape, the number of the clamping pieces is two, and the two clamping pieces are respectively arranged at the left end and the right end of the cover glass.
7. The slide structure for confocal microscopy according to claim 5, wherein: the clamping piece is a clip.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910921584.9A CN110673324B (en) | 2019-09-27 | 2019-09-27 | Glass slide structure suitable for confocal microscope |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910921584.9A CN110673324B (en) | 2019-09-27 | 2019-09-27 | Glass slide structure suitable for confocal microscope |
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| Publication Number | Publication Date |
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| CN110673324A true CN110673324A (en) | 2020-01-10 |
| CN110673324B CN110673324B (en) | 2022-04-26 |
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