CN204613505U - A kind of glass plate and cover glass set composite being applicable to laser confocal microscope - Google Patents
A kind of glass plate and cover glass set composite being applicable to laser confocal microscope Download PDFInfo
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- CN204613505U CN204613505U CN201520155265.9U CN201520155265U CN204613505U CN 204613505 U CN204613505 U CN 204613505U CN 201520155265 U CN201520155265 U CN 201520155265U CN 204613505 U CN204613505 U CN 204613505U
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Abstract
The utility model discloses a kind of glass plate and the cover glass set composite that are applicable to laser confocal microscope, it is characterized in that being made up of upper embedded groove, upper cover glass, glass plate, hole, lower cover glass and support slot etc.Upper embedded groove can prevent the landing of cover glass, and upper cover glass is easy to cover and open, and conveniently adjusts sample state, can reduce the murder by poisoning of noxious material to experimenter after upper cover glass covers; 2 holes are provided with in the middle part of glass plate, use lower cover glass by 2 holes blockings, hole and lower cover glass upper surface form airtight groove, and airtight groove can effectively prevent the toxic liquids such as clarifier from spilling, and avoid toxic liquid to the pollution of objective table and objective lens; Support slot can avoid lower cover glass lower surface contaminated.The utility model is easy to use, and work efficiency is high, and bottom portion of groove light transmission is fabulous, and Detection accuracy is high, is specially adapted to the instruments such as laser confocal microscope (inversion type) and inverted microscope.
Description
Technical field
The utility model relates to a kind of glass plate and the cover glass set composite that are applicable to laser confocal microscope, belongs to optical instrument glass experimental article technical field.
Background technology
The laser confocal microscope that current domestic many laboratories use is inversion type, needs in an experiment to use concave surface microslide carrying sample.Test for Embryos Development of Plant whole clearing Fluirescence observation, when carrying out embryonic development and dynamically observing, general operation step first in the groove of concave surface microslide, drips 1 ~ 2 clarifier or mountant, again ovule sample is placed in groove, drip several clarifiers as mountant and covered gently, and then concave surface microslide is reversed, be placed on objective table, the sample area at object lens alignment indentation place is focused and observed.But there is many problems in above-mentioned experimental implementation process: first, common concave surface microslide is when covering cover glass, and the sample in groove is easily shifted, and not easily focuses and search sample during later observations, cover glass covers rear being difficult to and adjusts sample state; Secondly, the unnecessary clarifier of dropping often can spill and overflow on objective table, easily pollutes objective table and objective lens; In addition, the clarifier (as wintergreen) used in experiment and fluorescent dye (as DAPI) etc. mostly have penetrating odor and have toxicity, and frequent contact can damage human body; Moreover in above experimental implementation process, test specimen is difficult to be recycled, for some rare species samples, add the difficulty of resampling undoubtedly.Needing development one at present badly can for the Novel glass plate of laser confocal microscope (inversion type) and cover glass set composite.
Summary of the invention
In order to solve the problem, the utility model provides a kind of glass plate and the cover glass set composite that are applicable to laser confocal microscope, this Novel glass plate and cover glass set composite make simple, easy for operation, 2 samples can be detected at every turn, and conveniently adjust sample state, substantially increase detection efficiency; The utility model glass plate and cover glass set composite can effectively stop toxic liquid to spill, and avoid the pollution of toxic liquid to objective table and object lens.
To achieve these goals, the technical scheme that the utility model adopts is, a kind of glass plate and cover glass set composite being applicable to laser confocal microscope, comprise upper embedded groove, upper cover glass, glass plate, hole, lower cover glass and support slot, described support slot is 40mm by 2 length, width is 5mm, thickness is the edge welt bonding formation of glass bar along glass plate minor face of 0.5mm, the upper surface of glass bar and the lower surface of glass plate use neutral gum bonding, and edge, glass bar long limit is parallel with glass plate minor face edge, described glass plate, glass bar is clear glass material, its upper surface and lower surface are planar structure, described glass plate length is 80mm, and width is 40mm, and thickness is 1mm, described upper embedded groove is 5mm by width, thickness be the glass bar of 0.5mm along a hollow groove of glass plate four bonding formation of edge welt, use neutral gum bonding between glass bar with glass plate.
The technical scheme that the utility model adopts is, one is applicable to glass plate that laser confocal microscope (inversion type) uses and cover glass set composite, comprises upper embedded groove, upper cover glass, glass plate, hole, lower cover glass and support slot composition.Portion is provided with upper embedded groove on a glass, described upper embedded groove is along the bonding hollow groove surrounded of glass plate four limit welts by glass bar, use neutral gum bonding between glass bar lower surface and glass plate upper surface, the landing of upper cover glass when described upper embedded groove can prevent glass plate from tilting; Described upper cover glass and lower cover glass are precleaning cover glass, thickness is 0.13 ~ 0.16mm, light transmission is fabulous, upper cover glass is easy to cover and open, be convenient to adjust sample state, after upper cover glass covers, can effectively stop toxic liquid to volatilize, reduce toxic liquid to the murder by poisoning of experiment operator; 2 holes are provided with in the middle part of glass plate, hole runs through the upper and lower surface of glass plate, the hole place on surface uses lower cover glass by two circular cavities shutoff under the glass plate, use neutral gum bonding between glass sheet lower surface and lower cover glass upper surface, the lower cover glass upper surface respective regions (bottom portion of groove) that wherein hole cross-sectional is corresponding does not use cementing agent, keeps its flat transparent structure; Hole and lower cover glass upper surface form circular airtight groove, and groove floor thickness is 0.13mm ~ 0.16mm, has fabulous light transmission, easily search sample and focusing when detecting; Airtight groove can prevent mountant from spilling, and efficiently avoid mountant and pollutes objective table and objective lens; At the lower surface bonding glass bar of welt respectively of two minor faces in glass plate bottom surface, use neutral gum bonding between glass sheet lower surface and the upper surface of glass bar, support slot is formed in the bottom of glass plate, support slot can prevent lower cover glass lower surface from contacting with desktop, in order to avoid pollute lower cover glass lower surface.
2 holes are provided with in the middle part of glass plate, 2 holes all run through the upper and lower surface of glass plate, the line of centres of 2 holes is parallel and vertical with the minor face of glass plate with two of glass plate long limits, and hole diameter is 6mm, and the central line distance of 2 holes is 10mm ~ 15mm.
Use lower cover glass by the holes blocking of 2 on glass plate, use neutral gum bonding between glass sheet lower surface with lower cover glass upper surface, the upper surface of 2 holes and lower cover glass forms 2 airtight grooves of circle, the lower cover glass surface area that described hole cross-sectional is corresponding does not all use cementing agent, keeps flat transparent structure.
The bottom portion of groove of described glass plate and cover glass set composite is planar structure, and bottom portion of groove thickness is 0.13 ~ 0.16mm, has good light transmission; Directly this is installed on objective table fixing when using low power microscopy; When using oily microscopy to survey, place sample in groove after, need this glass plate and the reversion of cover glass set composite, the lower cover glass lower surface respective regions corresponding at bottom portion of groove drips caryophyllus oil, and then this glass plate and cover glass set composite are reversed 180 °, to drip the regional alignment oil mirror of caryophyllus oil, micro regulating focusing is observed, and need not worry that in focus process, sample generation displacement and mountant spill problem.
As can be seen from above, the utility model a kind of be applicable to laser confocal microscope glass plate and cover glass set composite there is following beneficial effect:
1. the utility model glass plate and cover glass set composite are provided with upper embedded groove, upper cover glass landing when can effectively prevent glass plate from tilting; Upper cover glass directly covers on a glass, not only facilitates and adjusts sample state at any time, and can effectively stop harmful liquid to volatilize, and reduces harmful liquid to the murder by poisoning of experiment operator;
2. the glass plate of the utility model glass plate and cover glass set composite is provided with 2 holes, and hole and lower cover glass upper surface form 2 airtight grooves, and sample is placed in airtight groove and is not easy displacement, observation of easily searching during detection and focus; The closed structure of groove effectively can prevent the spilling of mountant, avoid mountant to pollute objective table and objective lens;
3. the utility model glass plate and cover glass set composite once can load 2 samples, drastically increase detection efficiency; Bottom portion of groove light transmission is fabulous, improves the accuracy of detection;
The utility model glass plate and cover glass set composite in use, are convenient to, to the sample recycling be detected, improve the utilization ratio of sample, decrease the trouble of resampling.
Accompanying drawing explanation
Fig. 1 is embodiment of the present invention longitudinal profile schematic diagram;
Fig. 2 is embodiment of the present invention front schematic view;
Fig. 3 is embodiment of the present invention reverse side (bottom surface) schematic diagram.
In figure, cover glass 2 upper embedded groove 3 glass plate 4 hole 5 neutral gum 6 times cover glass 7 support slots on 1
Embodiment
For making the object, technical solutions and advantages of the present invention clearly understand, below in conjunction with specific embodiment, and with reference to accompanying drawing, the present invention is described in more detail.
As shown in Figure 1, a kind of glass plate and cover glass set composite being applicable to laser confocal microscope of the utility model, comprises the compositions such as cover glass 1, upper embedded groove 2, glass plate 3, hole 4, neutral gum 5, lower cover glass 6, support slot 7.First with glass cutter, 1mm and 0.5mm clear glass is divided into required size glass plate and glass bar when making; Use electric drill and admantine drill (glass hole making drill) in the boring of glass plate centre position afterwards, hole diameter is 6mm, and hole runs through the upper and lower surface of glass plate, by glass plate, glass bar cleaning, cleans and dries rear for subsequent use.Next four limits glass bar being bonded in glass plate form hollow upper embedded groove, use neutral gum bonding between glass bar lower surface and glass plate upper surface; Bottom glass plate, form support slot on a glass by bonding for two glass bars, between glass bar upper surface and glass sheet lower surface, use neutral gum bonding; Then use lower cover glass by 2 holes blockings, neutral gum is used to bond together by between glass sheet lower surface and lower cover glass upper surface, the upper surface respective regions of the lower cover glass that hole cross-sectional is corresponding does not use cementing agent, keep this region flat transparent, fix with bonding rear clamp such as upper glass plates, glass bar and lower cover glasses and be placed in 45 DEG C of baking ovens and dry.
2 holes are provided with in the middle part of glass plate, 2 holes all run through the upper and lower surface of glass plate, the line of centres of 2 holes is parallel and vertical with the minor face of glass plate with two of glass plate long limits, and hole diameter is 6mm, and the central line distance of 2 holes is 10mm ~ 15mm.
As shown in Figure 1, be provided with 2 holes in the middle part of glass plate, these 2 holes run through whole glass plate, form 2 groove structures with the upper surface of lower cover glass; Bottom portion of groove light transmission is fabulous, is specially adapted to inverted microscope and laser confocal microscope, is very suitable for detecting bacterium liquid and solid sample etc.
When using the utility model set composite to carry out low power lens detection, first 1 ~ 2 clarifier (mountant) is dripped in groove, then sample to be positioned in groove and to suck unnecessary clarifier (mountant), upper cover glass is covered the upper surface at glass plate, afterwards this set composite is placed on objective table, focusing bottom low power lens alignment indentation is observed.When using oily microscopy to survey, first in groove, 1 ~ 2 clarifier or mountant is dripped, then sample is placed in groove, adjustment sample state also sucks unnecessary clarifier (mountant), due to surface tension of liquid absorption such as clarifiers, sample when being inverted by this set composite in groove can not drop easily, glass plate and cover glass combination unit are reversed 180 °, the lower cover glass lower surface respective regions corresponding at bottom portion of groove drips caryophyllus oil, and then this glass plate and cover glass combination unit are reversed 180 °, cover cover glass and whole device is placed on objective table, the zone focus dripping caryophyllus oil bottom oily mirror alignment indentation is observed, can also adjust sample state at any time as required in experimentation.
As mentioned above; those of ordinary skill in the field are to be understood that: the foregoing is only specific embodiments of the invention; be not limited to the present invention; within the spirit and principles in the present invention all; any amendment of making, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (4)
1. one kind is applicable to glass plate and the cover glass set composite of laser confocal microscope, it is characterized in that, comprise upper embedded groove, upper cover glass, glass plate, hole, lower cover glass and support slot, described support slot is 40mm by 2 length, width is 5mm, thickness is the edge welt bonding formation of glass bar along glass plate minor face of 0.5mm, the upper surface of glass bar and the lower surface of glass plate use neutral gum bonding, and edge, glass bar long limit is parallel with glass plate minor face edge, described glass plate, glass bar is clear glass material, its upper surface and lower surface are planar structure, described glass plate length is 80mm, and width is 40mm, and thickness is 1mm, described upper embedded groove is 5mm by width, thickness be the glass bar of 0.5mm along a hollow groove of glass plate four bonding formation of edge welt, use neutral gum bonding between glass bar with glass plate.
2. glass plate and the cover glass set composite being applicable to laser confocal microscope according to claim 1, it is characterized in that, 2 holes are provided with in the middle part of glass plate, 2 holes all run through the upper and lower surface of glass plate, the line of centres of 2 holes is parallel and vertical with the minor face of glass plate with two of glass plate long limits, hole diameter is 6mm, and the central line distance of 2 holes is 10mm ~ 15mm.
3. a kind of glass plate and cover glass set composite being applicable to laser confocal microscope according to claim 1, it is characterized in that, use lower cover glass by the holes blocking of 2 on glass plate, use neutral gum bonding between glass sheet lower surface with lower cover glass upper surface, the upper surface of 2 holes and lower cover glass forms 2 airtight grooves of circle, the lower cover glass surface area that described hole cross-sectional is corresponding does not all use cementing agent, keeps flat transparent structure.
4. glass plate and the cover glass set composite being applicable to laser confocal microscope according to claim 1, it is characterized in that, the bottom portion of groove of described glass plate and cover glass set composite is planar structure, and bottom portion of groove thickness is 0.13 ~ 0.16mm, has good light transmission; Directly this is installed on objective table fixing when using low power microscopy; When using oily microscopy to survey, place sample in groove after, need this glass plate and the reversion of cover glass set composite, the lower cover glass lower surface respective regions corresponding at bottom portion of groove drips caryophyllus oil, and then this glass plate and cover glass set composite are reversed 180 °, to drip the regional alignment oil mirror of caryophyllus oil, micro regulating focusing is observed, and need not worry that in focus process, sample generation displacement and mountant spill problem.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201520155265.9U CN204613505U (en) | 2015-03-18 | 2015-03-18 | A kind of glass plate and cover glass set composite being applicable to laser confocal microscope |
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| CN201520155265.9U CN204613505U (en) | 2015-03-18 | 2015-03-18 | A kind of glass plate and cover glass set composite being applicable to laser confocal microscope |
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Cited By (8)
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| CN106226141A (en) * | 2016-03-21 | 2016-12-14 | 广州深达生物制品技术有限公司 | A kind of slide glass cover plate for slide glass sample disposal and using method thereof |
| CN107063787A (en) * | 2016-12-19 | 2017-08-18 | 浙江世纪康大医疗科技股份有限公司 | A kind of automatic pullable cover plate system |
| CN110320080A (en) * | 2019-07-16 | 2019-10-11 | 宁夏医科大学总医院 | A kind of antigen high pressure steaming prosthetic device and restorative procedure |
| CN110376037A (en) * | 2019-08-02 | 2019-10-25 | 河北农业大学 | A kind of glass slide and operating method constructing larger tender tissue 3-D image based on laser confocal microscope |
| CN110673324A (en) * | 2019-09-27 | 2020-01-10 | 佛山科学技术学院 | Glass slide structure suitable for confocal microscope |
| CN111257302A (en) * | 2020-03-20 | 2020-06-09 | 中国科学院微生物研究所 | Liquid sample pool for detecting aerobic or facultative anaerobic pathogenic microorganisms by micro-Raman spectrum |
| CN113432945A (en) * | 2021-06-23 | 2021-09-24 | 长春汽车检测中心有限责任公司 | Device and method for preparing asbestos standard test piece for polarizing microscope |
| CN114488506A (en) * | 2020-11-12 | 2022-05-13 | 邑流微测股份有限公司 | Microscope observation platform |
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2015
- 2015-03-18 CN CN201520155265.9U patent/CN204613505U/en not_active Expired - Fee Related
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106226141A (en) * | 2016-03-21 | 2016-12-14 | 广州深达生物制品技术有限公司 | A kind of slide glass cover plate for slide glass sample disposal and using method thereof |
| WO2017162147A1 (en) * | 2016-03-21 | 2017-09-28 | 广州深达生物制品技术有限公司 | Slide cover for use in sample slide processing, and method utilizing same |
| CN107063787A (en) * | 2016-12-19 | 2017-08-18 | 浙江世纪康大医疗科技股份有限公司 | A kind of automatic pullable cover plate system |
| CN110320080A (en) * | 2019-07-16 | 2019-10-11 | 宁夏医科大学总医院 | A kind of antigen high pressure steaming prosthetic device and restorative procedure |
| CN110320080B (en) * | 2019-07-16 | 2022-01-25 | 宁夏医科大学总医院 | Antigen high-pressure steaming heat repair device and repair method |
| CN110376037A (en) * | 2019-08-02 | 2019-10-25 | 河北农业大学 | A kind of glass slide and operating method constructing larger tender tissue 3-D image based on laser confocal microscope |
| CN110673324A (en) * | 2019-09-27 | 2020-01-10 | 佛山科学技术学院 | Glass slide structure suitable for confocal microscope |
| CN111257302A (en) * | 2020-03-20 | 2020-06-09 | 中国科学院微生物研究所 | Liquid sample pool for detecting aerobic or facultative anaerobic pathogenic microorganisms by micro-Raman spectrum |
| CN111257302B (en) * | 2020-03-20 | 2021-05-04 | 中国科学院微生物研究所 | Liquid sample pool for detecting aerobic or facultative anaerobic pathogenic microorganisms by micro-Raman spectrum |
| CN114488506A (en) * | 2020-11-12 | 2022-05-13 | 邑流微测股份有限公司 | Microscope observation platform |
| CN113432945A (en) * | 2021-06-23 | 2021-09-24 | 长春汽车检测中心有限责任公司 | Device and method for preparing asbestos standard test piece for polarizing microscope |
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| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150902 Termination date: 20180318 |