CN103235407A - Multi-sample stage allowing for automatic movement of inverted microscopes - Google Patents
Multi-sample stage allowing for automatic movement of inverted microscopes Download PDFInfo
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- CN103235407A CN103235407A CN201310114113XA CN201310114113A CN103235407A CN 103235407 A CN103235407 A CN 103235407A CN 201310114113X A CN201310114113X A CN 201310114113XA CN 201310114113 A CN201310114113 A CN 201310114113A CN 103235407 A CN103235407 A CN 103235407A
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Abstract
The invention relates to a multi-sample stage allowing for automatic movement of inverted microscopes. The multi-sample stage comprises a sample stage base and an objective lens. Screw supports are arranged on the sample stage base. A screw is arranged on the two screw supports through a coupling. The coupling is connected with a drive motor. A slide is arranged on the sample stage base. A sample cell moving rack is arranged on the slide. The top of the sample cell moving rack is screwed to the screw through a connector. A sample cell fixed rack is arranged on the sample stage base and is provided with a sample cell. The upper surface of the sample cell is made of a quartz glass slide, and the lower surface of the sample cell is made of a cover glass. The periphery of each of the quartz glass slide and the cover glass is sealed with epoxy resin. Space between the quartz slide glass and the cover glass is divided into a plurality of independent sample channels. The quartz glass slide is provided with through holes corresponding to the independent sample channels. The through holes are connected with an automatic sample feeder through sample guide tubes. The multi-sample stage is applicable to various inverted microscopes, is small in size, handy, flexible, and simple and fast to operate, and is an effective tool for the biological single-molecular study.
Description
Technical field
The invention belongs to the biological scientific experiment field, is a kind of unimolecule to be detected and the device of handling, and is a kind of inverted microscope mobile various product objective table automatically specifically.
Background technology
The unimolecule technology refers on single molecules level behavior to biomacromolecule (comprising conformation change, interaction, identification etc. mutually) and carries out Real-time and Dynamic Detection and the technology that biomacromolecule is handled and regulates and control on this basis, and the unimolecule technology has advantages such as direct, accurate, real-time.We can be by observing the behavior of unimolecule such as single enzyme molecule, RNA/DNA molecule, the elastic property of research nucleic acid molecules and the structural change that external force causes; The unimolecule technology also can provide many specific informations that are different from the molecular aggregates attitude, study the flexibility of fluctuation, biomacromolecule of special local environment, the molecule of individual molecule and motion thereof etc. for people and created advantage, huge impetus has been played in the development of biological detection.
The unimolecule technology is to have grown up since the nineties in last century, for monomolecular detection and manipulation, technology such as light tweezer, magnetic tweezer, atomic force microscope, glass microtubule and molecular comb have been introduced, add the single molecular fluorescence technology, make people can directly detect motion and the variation of observing and handling individual molecule, or by applying the process that external force changes biochemical reaction, mutual conversion between research chemical energy and the mechanical energy, this direct observation and artificial controlled advantage make the unimolecule technology develop rapidly becomes very important means in the biophysics research.But monomolecular complex operation is consuming time, poor repeatability; In the unimolecule experiment, certain hour will cause surface failure because of airborne moisture or dust etc. in the air if the molecular surface of the long-time special processing of process is exposed to; In addition, for improving conventional efficient, must be provided with a plurality of sample cells simultaneously on the microslide in the unimolecule experiment, but when experimental implementation, because microslide need fix and make the sample cell that must observe just in time to be positioned at the object lens top, and traditional objective table moving range only limits to a sample cell, can not satisfy the requirement of changing rapidly between a plurality of sample cells, so need to dismantle microslide (sample cell) before each application of sample, install again behind the position to be replaced, complicated operation, time and effort consuming.
A kind of alternative inverted microscope for Single Molecule Detection and manipulation is arranged now.Inverted microscope is the same with simple microscope, and only object lens and illuminator are put upside down, and the former is under objective table, and the latter is on objective table.Because inverted microscope is furnished with condenser, long reach flat-field achromatic objective lens and the contrast unit of long reach, so can use various double dish and culture flask, be specially adapted to active somatic cell and tissue, liquid, sediment etc. are carried out microscopy.According to different inverted microscopes, sample stage is also simplified and traditional different with building of sample cell, and applicable scope is also had nothing in common with each other.Also there is not at present a kind of inverted microscope that is applicable to Single Molecule Detection and manipulation fully, mainly be observed sample in the focal plane of micro objective replacing and the focusing too loaded down with trivial details, and can not be provided with a plurality of sample cells simultaneously, can not satisfy the requirement of changing rapidly between a plurality of sample cells, be not easy to observe unimolecule with fluorescent method.
Summary of the invention
The objective of the invention is to be not easy to be observed sample and in the focal plane of micro objective, to change and focus and to be provided with the defective of a plurality of sample cells simultaneously in order to overcome existing inverted microscope objective table, invent various product objective table of the automatic movement of a kind of inverted microscope.
The objective of the invention is to realize as follows: various product objective table that described inverted microscope moves automatically, comprise sample platform base, sample platform base central authorities are provided with the object lens mount pad, be provided with object lens below the object lens mount pad, it is characterized in that: on sample platform base, respectively be provided with a screw rod support in the both sides of object lens mount pad, screw rod is located on the two screw rod supports by shaft coupling, and shaft coupling is connected with drive motor; Be provided with slide rail at sample platform base, slide rail is provided with the sample cell movable stand, and the top of sample cell movable stand is screwed onto on the screw rod by joint, will drive the side-to-side movement on slide rail of sample cell movable stand when screw rod rotates; Be provided with the sample cell fixed mount at sample platform base, the sample cell fixed mount is provided with sample cell, the upper surface of sample cell is quartzy microslide, lower surface is cover glass, the periphery of quartzy microslide and cover glass seals with epoxy resin, be divided into a plurality of independent sample passages between quartzy microslide and the cover glass, quartzy microslide is provided with the through hole corresponding with the independent sample passage, through hole is connected with automatic sampler by sample line, and the automatic sampler that testing sample passes through enters and flows out sample cell.
A side that is positioned at two screw rod supports on the described sample platform base respectively is provided with a position limitation protection switch; the sample cell movable stand is clipped between two position limitation protection switches; when the sample cell movable stand moves to the position of screw rod support; under the effect of position limitation protection switch, with stop motion, move round about then.
The square slide that described quartzy microslide is 25X75mm; The diameter of described through hole is 0.9mm.
Described drive motor is stepper drive motors.
Good effect of the present invention is as follows: the present invention can control according to actual needs and place the sample that is observed of sample cell to move in the focal plane of micro objective, simplified the process that sample cell is changed and focused, it is more simple and direct efficient to make the application fluorescent method observe unimolecule.The present invention is applicable to various inverted microscope, and its volume is little, tests convenient, flexiblely, and behaviour does and changes simple and directly, can be used for the quick-replaceable experiments of the many sample cells of needs such as single molecular fluorescence technology, magnetic tweezer, light tweezer and cell experiment; The present invention can realize accurate location, has bigger conversion observation scope, is the efficient tool of biologic single molecular when researching and analysing.
Description of drawings
Fig. 1 is master's TV structure figure of the present invention
Fig. 2 is schematic appearance of the present invention
Among the figure: 1 sample platform base, 2 object lens mount pads, 3 object lens
4 screw rod supports, 5 screw rods, 6 shaft couplings
7 drive motor, 8 slide rails, 9 sample cell movable stands
10 joints, 11 sample cell fixed mounts, 12 sample cells
13 quartzy microslide 14 cover glasses 15 independent sample passages
16 sample line, 17 automatic samplers, 18 position limitation protection switches
18 position limitation protection switches
Embodiment
Various product objective table that described inverted microscope moves automatically, comprise sample platform base 1, sample platform base 1 central authorities are provided with object lens mount pad 2, be provided with object lens 3 below the object lens mount pad 2, it is characterized in that: on sample platform base 1, respectively be provided with a screw rod support 4 in the both sides of object lens mount pad 2, screw rod 5 is located on the two screw rod supports by shaft coupling 6, and shaft coupling 6 is connected with drive motor 7; Be provided with slide rail 8 at sample platform base 1, slide rail 8 is provided with sample cell movable stand 9, and the top of sample cell movable stand 9 is screwed onto on the screw rod 5 by joint 10, will drive sample cell movable stand 9 side-to-side movement on slide rail 8 when screw rod 5 rotates; Be provided with sample cell fixed mount 11 at sample platform base 1, sample cell fixed mount 11 is provided with sample cell 12, the upper surface of sample cell 12 is quartzy microslide 13, lower surface is cover glass 14, the periphery of quartzy microslide 13 and cover glass 14 seals with epoxy resin, be divided into a plurality of independent sample passages 15 between quartzy microslide 13 and the cover glass 14, quartzy microslide 13 is provided with the through hole corresponding with independent sample passage 15, through hole is connected with automatic sampler 17 by sample line 16, and the automatic sampler 17 that testing sample passes through enters and flows out sample cell 12.
A side that is positioned at two screw rod supports 4 on the described sample platform base 1 respectively is provided with a position limitation protection switch 18; sample cell movable stand 9 is clipped between two position limitation protection switches 18; when sample cell movable stand 9 moves to the position of screw rod support 4; under the effect of position limitation protection switch 13, with stop motion, move round about then.
Described quartzy microslide 13 is the square slide of 25X75mm; The diameter of described through hole is 0.9mm.
Described drive motor 7 is stepper drive motors.
The course of work of the present invention is as follows:
At first, to put into sample cell fixed mount 11 and fixing through the sample cell 12 of cleaning and finishing, regulate sample cell movable stand 9 then, sample cell 12 is same moved further thereupon, the independent sample passage 15 that the needs on the sample cell 12 are observed be positioned at object lens 3 directly over.Regulate object lens 3 focal lengths, make that the target molecule on surface of quartzy microslide 13 is high-visible.Testing sample injects independent sample passage 15 fast by automatic sampler 17 and sample line 16, and can observe and practical CCD recording track simultaneously.Observe when this independent sample passage 15 and to finish, control drive motor 7 is sample cell 12 translation setpoint distances, and the independent sample passage 15 that makes the next one to observe is positioned at position directly over the object lens just.Following steps then as above repeat to finish until 12 all independent sample passages 15 observations of this sample cell.
Claims (4)
1. an inverted microscope mobile various product objective table automatically, comprise sample platform base, sample platform base central authorities are provided with the object lens mount pad, be provided with object lens below the object lens mount pad, it is characterized in that: on sample platform base, respectively be provided with a screw rod support in the both sides of object lens mount pad, screw rod is located on the two screw rod supports by shaft coupling, and shaft coupling is connected with drive motor; Be provided with slide rail at sample platform base, slide rail is provided with the sample cell movable stand, and the top of sample cell movable stand is screwed onto on the screw rod by joint, will drive the side-to-side movement on slide rail of sample cell movable stand when screw rod rotates; Be provided with the sample cell fixed mount at sample platform base, the sample cell fixed mount is provided with sample cell, the upper surface of sample cell is quartzy microslide, lower surface is cover glass, the periphery of quartzy microslide and cover glass seals with epoxy resin, be divided into a plurality of independent sample passages between quartzy microslide and the cover glass, quartzy microslide is provided with the through hole corresponding with the independent sample passage, through hole is connected with automatic sampler by sample line, and the automatic sampler that testing sample passes through enters and flows out sample cell.
2. the automatic mobile various product objective table of inverted microscope according to claim 1; it is characterized in that: a side that is positioned at two screw rod supports on the described sample platform base respectively is provided with a position limitation protection switch; the sample cell movable stand is clipped between two position limitation protection switches; when the sample cell movable stand moves to the position of screw rod support; under the effect of position limitation protection switch, with stop motion, move round about then.
3. the automatic mobile various product objective table of inverted microscope according to claim 1 is characterized in that: the square slide that described quartzy microslide is 25X75mm; The diameter of described through hole is 0.9mm.
4. the automatic mobile various product objective table of inverted microscope according to claim 1, it is characterized in that: described drive motor is stepper drive motors.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201310114113.XA CN103235407B (en) | 2013-04-03 | 2013-04-03 | The Multi-example objective table of inverted microscope movement automatically |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310114113.XA CN103235407B (en) | 2013-04-03 | 2013-04-03 | The Multi-example objective table of inverted microscope movement automatically |
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| CN103235407A true CN103235407A (en) | 2013-08-07 |
| CN103235407B CN103235407B (en) | 2015-11-25 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104931419A (en) * | 2015-05-28 | 2015-09-23 | 中国科学院物理研究所 | Transverse magnetic tweezer device |
| CN106537223A (en) * | 2014-07-09 | 2017-03-22 | 文塔纳医疗系统公司 | Automated coverslipper and methods of use |
| TWI579588B (en) * | 2015-09-04 | 2017-04-21 | 中原大學 | Microscope monitoring device and system thereof |
| CN106772985A (en) * | 2016-12-01 | 2017-05-31 | 东莞市天合机电开发有限公司 | A kind of microscope adjusting bracket |
| CN107063977A (en) * | 2017-04-23 | 2017-08-18 | 湖南乾康科技有限公司 | A kind of multichannel counting chamber |
| CN108067314A (en) * | 2017-12-21 | 2018-05-25 | 重庆骄直电气有限公司 | Use for laboratory operation console |
| CN108398420A (en) * | 2018-01-31 | 2018-08-14 | 华南理工大学 | The detection device of luminescent material mechanoluminescence performance |
| CN109696403A (en) * | 2017-10-23 | 2019-04-30 | 中国科学院重庆绿色智能技术研究院 | A kind of sample room for immersion micro-imaging |
| CN110220895A (en) * | 2019-06-28 | 2019-09-10 | 中国计量大学 | A kind of image collecting device of high throughput portable colloidal gold CCD readout instrument |
| CN110441899A (en) * | 2019-07-24 | 2019-11-12 | 安徽新知数媒信息科技有限公司 | A kind of travelling shot regulating device based on optical microscopy |
| CN110441900A (en) * | 2019-07-24 | 2019-11-12 | 安徽新知数媒信息科技有限公司 | A kind of travelling shot method based on optical microscopy |
| CN113406362A (en) * | 2021-06-16 | 2021-09-17 | 北京航空航天大学合肥创新研究院(北京航空航天大学合肥研究生院) | High vacuum normal position atomic force microscope sample presentation device |
| CN114088606A (en) * | 2021-10-23 | 2022-02-25 | 广州市艾贝泰生物科技有限公司 | Cell analyzer |
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| US20020104231A1 (en) * | 2001-02-05 | 2002-08-08 | Tamotsu Tominaga | Position measuring apparatus |
| CN1391121A (en) * | 2002-06-21 | 2003-01-15 | 中国科学院上海生命科学研究院 | Movable objective table for electrobiologic microscope |
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Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106537223B (en) * | 2014-07-09 | 2019-02-15 | 文塔纳医疗系统公司 | Automatic cover plate machine and application method |
| CN106537223A (en) * | 2014-07-09 | 2017-03-22 | 文塔纳医疗系统公司 | Automated coverslipper and methods of use |
| CN104931419A (en) * | 2015-05-28 | 2015-09-23 | 中国科学院物理研究所 | Transverse magnetic tweezer device |
| CN104931419B (en) * | 2015-05-28 | 2018-03-02 | 中国科学院物理研究所 | Horizontal magnetic tweezers |
| TWI579588B (en) * | 2015-09-04 | 2017-04-21 | 中原大學 | Microscope monitoring device and system thereof |
| CN106772985A (en) * | 2016-12-01 | 2017-05-31 | 东莞市天合机电开发有限公司 | A kind of microscope adjusting bracket |
| CN106772985B (en) * | 2016-12-01 | 2019-10-22 | 罗芳芳 | A kind of microscope adjusting bracket |
| CN107063977A (en) * | 2017-04-23 | 2017-08-18 | 湖南乾康科技有限公司 | A kind of multichannel counting chamber |
| CN109696403A (en) * | 2017-10-23 | 2019-04-30 | 中国科学院重庆绿色智能技术研究院 | A kind of sample room for immersion micro-imaging |
| CN109696403B (en) * | 2017-10-23 | 2021-08-13 | 中国科学院重庆绿色智能技术研究院 | Sample chamber for immersed microscopic imaging |
| CN108067314A (en) * | 2017-12-21 | 2018-05-25 | 重庆骄直电气有限公司 | Use for laboratory operation console |
| CN108398420A (en) * | 2018-01-31 | 2018-08-14 | 华南理工大学 | The detection device of luminescent material mechanoluminescence performance |
| CN110220895A (en) * | 2019-06-28 | 2019-09-10 | 中国计量大学 | A kind of image collecting device of high throughput portable colloidal gold CCD readout instrument |
| CN110441899A (en) * | 2019-07-24 | 2019-11-12 | 安徽新知数媒信息科技有限公司 | A kind of travelling shot regulating device based on optical microscopy |
| CN110441900A (en) * | 2019-07-24 | 2019-11-12 | 安徽新知数媒信息科技有限公司 | A kind of travelling shot method based on optical microscopy |
| CN113406362A (en) * | 2021-06-16 | 2021-09-17 | 北京航空航天大学合肥创新研究院(北京航空航天大学合肥研究生院) | High vacuum normal position atomic force microscope sample presentation device |
| CN114088606A (en) * | 2021-10-23 | 2022-02-25 | 广州市艾贝泰生物科技有限公司 | Cell analyzer |
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