CN103235407B - The Multi-example objective table of inverted microscope movement automatically - Google Patents
The Multi-example objective table of inverted microscope movement automatically Download PDFInfo
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- CN103235407B CN103235407B CN201310114113.XA CN201310114113A CN103235407B CN 103235407 B CN103235407 B CN 103235407B CN 201310114113 A CN201310114113 A CN 201310114113A CN 103235407 B CN103235407 B CN 103235407B
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Abstract
The present invention is the Multi-example objective table of a kind of inverted microscope movement automatically, and comprise sample stage base and object lens, sample stage base is provided with screw rod support, screw rod is located on two screw rod supports by shaft coupling, and shaft coupling is connected with drive motor; Sample stage base is provided with slide rail, and slide rail is provided with sample cell movable stand, and the top of sample cell movable stand is screwed onto on screw rod by joint; Sample stage base is provided with sample cell fixed mount, sample cell fixed mount is provided with sample cell, the upper surface of sample cell is quartz slide, lower surface is cover glass, the periphery epoxy resin sealing of quartz slide and cover glass, be divided into multiple independent sample passages between quartz slide and cover glass, quartz slide is provided with the through hole corresponding with independent sample passage, and through hole is connected with automatic sampler by sample line.The present invention is applicable to various inverted microscope, and its volume is little, convenient, flexible, simple to operation, is the efficient tool of biologic single molecular research.
Description
Technical field
The invention belongs to biological scientific experiment field, is a kind of device unimolecule being carried out to detect Yu handle, and is the Multi-example objective table of a kind of inverted microscope movement automatically specifically.
Background technology
Single molecule techniques refers to and carry out Real-time and Dynamic Detection and the technology handled biomacromolecule and regulate and control on this basis to the behavior (comprise conformation change, interaction, mutually identify) of biomacromolecule on single molecules level, and single molecule techniques has the advantages such as direct, accurate, real-time.We can by observation unimolecule as the behavior of single enzyme molecule, RNA/DNA molecule, the structure change that the elastic property of research nucleic acid molecules and external force cause; Single molecule techniques also can provide many specific informations being different from molecular aggregation state, for people study the special local environment of individual molecule, the fluctuation of molecule, the flexibility of biomacromolecule and motion etc. thereof create advantage, serves huge impetus to the development of biological detection.
Single molecule techniques has grown up since being the nineties in last century, in order to monomolecular detection and manipulation, introduce the technology such as light tweezer, magnetic tweezer, atomic force microscope, glass-micropipe and molecular comb, add single molecular fluorescence technology, people's direct-detection is observed and handles motion and the change of individual molecule, or the process of biochemical reaction is changed by applying external force, mutual conversion between specializes in chemistry energy and mechanical energy, this direct observation and artificially controlled advantage make single molecule techniques develop rapidly becomes very important means in biophysics research.But monomolecular complex operation is consuming time, poor repeatability; In single molecule experiments, if be exposed to certain hour in air through the molecular surface of long-time special processing will cause surface failure because of the moisture in air or dust etc.; In addition, for improving conventional efficient, in single molecule experiments, microslide must be provided with multiple sample cell simultaneously, but when experimental implementation, because microslide needs fix and make the sample cell that must observe just in time be positioned at above object lens, and traditional objective table moving range is only limitted to a sample cell, the requirement changed rapidly between multiple sample cell can not be met, so need dismounting microslide (sample cell) before each application of sample, install again behind position to be replaced, complicated operation, time and effort consuming.
There is now a kind of alternative inverted microscope for Single Molecule Detection and manipulation.Inverted microscope is the same with simple microscope, and only object lens and illuminator are put upside down, and the former is under objective table, and the latter is on objective table.Because inverted microscope is furnished with the condenser of long reach, long reach flat-field achromatic objective lens and contrast unit, therefore various double dish and culture flask can be used, be specially adapted to carry out microscopy to active somatic cell and tissue, liquid, sediment etc.According to different inverted microscopes, sample stage is also simplified and traditional different from building of sample cell, and the scope be suitable for also is had nothing in common with each other.Also there is no a kind of inverted microscope being applicable to Single Molecule Detection and manipulation completely at present, mainly be observed the replacing of sample in the focal plane of micro objective and focusing is too loaded down with trivial details, and multiple sample cell can not be provided with simultaneously, the requirement changed rapidly between multiple sample cell can not be met, be not easy to observe unimolecule with fluorescent method.
Summary of the invention
The object of the invention is to be not easy to be observed sample and to change in the focal plane of micro objective and focus to overcome existing inverted microscope objective table and can not be provided with the defect of multiple sample cell simultaneously, invent the Multi-example objective table of a kind of inverted microscope movement automatically.
The object of the invention is to realize as follows: the Multi-example objective table of described inverted microscope movement automatically, comprise sample stage base, sample stage base central authorities are provided with object lens mount pad, object lens are provided with below object lens mount pad, it is characterized in that: on sample stage base, be respectively provided with a screw rod support in the both sides of object lens mount pad, screw rod is located on two screw rod supports by shaft coupling, and shaft coupling is connected with drive motor; Sample stage base is provided with slide rail, and slide rail is provided with sample cell movable stand, and the top of sample cell movable stand is screwed onto on screw rod by joint, will drive the side-to-side movement on slide rail of sample cell movable stand when screw rod rotates; Sample stage base is provided with sample cell fixed mount, sample cell fixed mount is provided with sample cell, the upper surface of sample cell is quartz slide, lower surface is cover glass, the periphery epoxy resin sealing of quartz slide and cover glass, multiple independent sample passages is divided between quartz slide and cover glass, quartz slide is provided with the through hole corresponding with independent sample passage, through hole is connected with automatic sampler by sample line, and the automatic sampler that testing sample passes through enters and flows out sample cell.
The side described sample stage base being positioned at two screw rod supports is respectively provided with a position limitation protection switch; sample cell movable stand is clipped between two position limitation protection switches; when sample cell movable stand moves to the position of screw rod support; by stop motion under the effect of position limitation protection switch, then move round about.
Described quartz slide is the square slide of 25X75mm; The diameter of described through hole is 0.9mm.
Described drive motor is stepper drive motors.
Good effect of the present invention is as follows: the present invention can manipulate according to actual needs the sample that is observed being placed in sample cell and move in the focal plane of micro objective, simplify the process that sample cell is changed and focused, make application fluorescent method observe unimolecule more efficiently simple and direct.The present invention is applicable to various inverted microscope, and its volume is little, tests convenient, flexible, and it is simple and direct that behaviour does replacing, can be used for single molecular fluorescence technology, magnetic tweezer, light tweezer and cell experiment etc. and need the quick-replaceable experiment in Multi-example pond; The present invention can realize accurate location, has larger conversion observation scope, is the efficient tool of biologic single molecular when researching and analysing.
Accompanying drawing explanation
Fig. 1 is the main TV structure figure of the present invention
Fig. 2 is schematic appearance of the present invention
In figure: 1 sample stage base 2 object lens mount pad 3 object lens
4 screw rod support 5 screw rod 6 shaft couplings
7 drive motor 8 slide rail 9 sample cell movable stands
10 joint 11 sample cell fixed mount 12 sample cells
13 quartz slide 14 cover glass 15 independent sample passages
16 sample line 17 automatic sampler 18 position limitation protection switches
18 position limitation protection switches
Embodiment
The Multi-example objective table of described inverted microscope movement automatically, comprise sample stage base 1, sample stage base 1 central authorities are provided with object lens mount pad 2, object lens mount pad 2 is provided with object lens 3 below, it is characterized in that: on sample stage base 1, be respectively provided with a screw rod support 4 in the both sides of object lens mount pad 2, screw rod 5 is located on two screw rod supports by shaft coupling 6, and shaft coupling 6 is connected with drive motor 7; Sample stage base 1 is provided with slide rail 8, and slide rail 8 is provided with sample cell movable stand 9, and the top of sample cell movable stand 9 is screwed onto on screw rod 5 by joint 10, will drive sample cell movable stand 9 side-to-side movement on slide rail 8 when screw rod 5 rotates; Sample stage base 1 is provided with sample cell fixed mount 11, sample cell fixed mount 11 is provided with sample cell 12, the upper surface of sample cell 12 is quartz slide 13, lower surface is cover glass 14, the periphery epoxy resin sealing of quartz slide 13 and cover glass 14, multiple independent sample passages 15 is divided between quartz slide 13 and cover glass 14, quartz slide 13 is provided with the through hole corresponding with independent sample passage 15, through hole is connected with automatic sampler 17 by sample line 16, and the automatic sampler 17 that testing sample passes through enters and flows out sample cell 12.
The side described sample stage base 1 being positioned at two screw rod supports 4 is respectively provided with a position limitation protection switch 18; sample cell movable stand 9 is clipped between two position limitation protection switches 18; when sample cell movable stand 9 moves to the position of screw rod support 4; by stop motion under the effect of position limitation protection switch 13, then move round about.
Described quartz slide 13 is the square slide of 25X75mm; The diameter of described through hole is 0.9mm.
Described drive motor 7 is stepper drive motors.
The course of work of the present invention is as follows:
First, sample cell 12 through cleaning and finishing is put into sample cell fixed mount 11 and fixes, then regulate sample cell movable stand 9, sample cell 12 synchronizing moving thereupon, the independent sample passage 15 that the needs on sample cell 12 are observed is positioned at directly over object lens 3.Regulate object lens 3 focal length, make the target molecule on the surface of quartz slide 13 high-visible.Testing sample injects independent sample passage 15 fast by automatic sampler 17 and sample line 16, and can carry out observing and practical CCD recording track simultaneously.Observe when this independent sample passage 15 and terminate, control drive motor 7 by sample cell 12 translation setpoint distance, the independent sample passage 15 that the next one will be observed is positioned at position directly over object lens just.Following steps then as above repeat until all independent sample passages 15 of this sample cell 12 have been observed.
Claims (4)
1. the Multi-example objective table of inverted microscope movement automatically, comprise sample stage base, sample stage base central authorities are provided with object lens mount pad, object lens are provided with below object lens mount pad, it is characterized in that: on sample stage base, be respectively provided with a screw rod support in the both sides of object lens mount pad, screw rod is located on two screw rod supports by shaft coupling, and shaft coupling is connected with drive motor; Sample stage base is provided with slide rail, and slide rail is provided with sample cell movable stand, and the top of sample cell movable stand is screwed onto on screw rod by joint, will drive the side-to-side movement on slide rail of sample cell movable stand when screw rod rotates; Sample stage base is provided with sample cell fixed mount, sample cell fixed mount is provided with sample cell, regulate sample cell movable stand, sample cell is synchronizing moving thereupon, the upper surface of sample cell is quartz slide, lower surface is cover glass, the periphery epoxy resin sealing of quartz slide and cover glass, multiple independent sample passages is divided between quartz slide and cover glass, quartz slide is provided with the through hole corresponding with independent sample passage, through hole is connected with automatic sampler by sample line, and the automatic sampler that testing sample passes through enters and flows out sample cell.
2. the Multi-example objective table of inverted microscope according to claim 1 movement automatically; it is characterized in that: the side described sample stage base being positioned at two screw rod supports is respectively provided with a position limitation protection switch; sample cell movable stand is clipped between two position limitation protection switches; when sample cell movable stand moves to the position of screw rod support; by stop motion under the effect of position limitation protection switch, then move round about.
3. the Multi-example objective table of inverted microscope according to claim 1 movement automatically, is characterized in that: described quartz slide is the square slide of 25X75mm; The diameter of described through hole is 0.9mm.
4. the Multi-example objective table of inverted microscope according to claim 1 movement automatically, is characterized in that: described drive motor is stepper drive motors.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310114113.XA CN103235407B (en) | 2013-04-03 | 2013-04-03 | The Multi-example objective table of inverted microscope movement automatically |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310114113.XA CN103235407B (en) | 2013-04-03 | 2013-04-03 | The Multi-example objective table of inverted microscope movement automatically |
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| CN103235407A CN103235407A (en) | 2013-08-07 |
| CN103235407B true CN103235407B (en) | 2015-11-25 |
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Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
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| DK3167331T3 (en) * | 2014-07-09 | 2018-08-06 | Ventana Med Syst Inc | AUTOMATED COVER GLASS APPLICATIONS AND METHODS OF USE |
| CN104931419B (en) * | 2015-05-28 | 2018-03-02 | 中国科学院物理研究所 | Horizontal magnetic tweezers |
| TWI579588B (en) * | 2015-09-04 | 2017-04-21 | 中原大學 | Microscope monitoring device and system thereof |
| CN106772985B (en) * | 2016-12-01 | 2019-10-22 | 罗芳芳 | A kind of microscope adjusting bracket |
| CN107063977A (en) * | 2017-04-23 | 2017-08-18 | 湖南乾康科技有限公司 | A kind of multichannel counting chamber |
| CN109696403B (en) * | 2017-10-23 | 2021-08-13 | 中国科学院重庆绿色智能技术研究院 | Sample chamber for immersed microscopic imaging |
| CN108067314A (en) * | 2017-12-21 | 2018-05-25 | 重庆骄直电气有限公司 | Use for laboratory operation console |
| CN108398420B (en) * | 2018-01-31 | 2021-01-19 | 华南理工大学 | Device for detecting force-induced luminescence performance of luminescent material |
| CN110220895A (en) * | 2019-06-28 | 2019-09-10 | 中国计量大学 | A kind of image collecting device of high throughput portable colloidal gold CCD readout instrument |
| CN110441899A (en) * | 2019-07-24 | 2019-11-12 | 安徽新知数媒信息科技有限公司 | A kind of travelling shot regulating device based on optical microscopy |
| CN110441900A (en) * | 2019-07-24 | 2019-11-12 | 安徽新知数媒信息科技有限公司 | A kind of travelling shot method based on optical microscopy |
| CN113406362A (en) * | 2021-06-16 | 2021-09-17 | 北京航空航天大学合肥创新研究院(北京航空航天大学合肥研究生院) | High vacuum normal position atomic force microscope sample presentation device |
| CN114088606B (en) * | 2021-10-23 | 2023-05-09 | 广州市艾贝泰生物科技有限公司 | Cell analysis device |
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| CN1391121A (en) * | 2002-06-21 | 2003-01-15 | 中国科学院上海生命科学研究院 | Movable objective table for electrobiologic microscope |
| CN101042326A (en) * | 2007-04-16 | 2007-09-26 | 中国科学院物理研究所 | Total reflection near-field microscope combining with magnetic forceps for observing biomacromolecule |
| CN202748529U (en) * | 2012-04-09 | 2013-02-20 | 哈尔滨医科大学 | Objective table capable of automatically rotating, positioning and culturing |
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| TW528881B (en) * | 2001-02-05 | 2003-04-21 | Hitachi Int Electric Inc | Position measuring apparatus |
| DE102010061167B3 (en) * | 2010-12-10 | 2012-05-31 | Leica Microsystems Cms Gmbh | microscope stage |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1391121A (en) * | 2002-06-21 | 2003-01-15 | 中国科学院上海生命科学研究院 | Movable objective table for electrobiologic microscope |
| CN101042326A (en) * | 2007-04-16 | 2007-09-26 | 中国科学院物理研究所 | Total reflection near-field microscope combining with magnetic forceps for observing biomacromolecule |
| CN202748529U (en) * | 2012-04-09 | 2013-02-20 | 哈尔滨医科大学 | Objective table capable of automatically rotating, positioning and culturing |
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