CN105004702A - Dual-imaging magnetic tweezer system - Google Patents
Dual-imaging magnetic tweezer system Download PDFInfo
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- CN105004702A CN105004702A CN201510340396.9A CN201510340396A CN105004702A CN 105004702 A CN105004702 A CN 105004702A CN 201510340396 A CN201510340396 A CN 201510340396A CN 105004702 A CN105004702 A CN 105004702A
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Abstract
The invention provides a dual-imaging magnetic tweezer system. The dual-imaging magnetic tweezer system comprises a bearing device, a magnetic tweezer device, a microscopic measuring and controlling device, a microscopic imaging device and a control device, wherein the bearing device bears sample molecules; the magnetic tweezer device performs mechanical manipulation on the sample molecules to enable the sample molecules to move; the microscopic measuring and controlling device collects first image data of the movement of the sample molecules from the side faces of the sample molecules; the microscopic imaging device collects second image data of the movement of the sample molecules from the positions above the sample molecules; the control device analyzes the first image data to obtain a first analysis result, analyzes the second image data to obtain a second analysis result and verifies the first analysis result and the second analysis result to determine a result of the sample molecules changing with time in the three-dimensional space. Accordingly, fluorescence signal detection can be performed on the sample molecules through the microscopic measuring and controlling device, and meanwhile the mechanical manipulation can be performed on the sample molecules through the microscopic imaging device; in addition, verification can be performed according to the obtained first image data and the obtained second image data, and the test precision is improved.
Description
Technical field
The invention belongs to micro-detection technique field, particularly relate to a kind of two imaging magnetic tweezers system.
Background technology
In the dynamic process that micro-nano-scale studies biomolecule and cell, the conformation of research molecule and conformation over time, can disclose the secret of vital movement further.
In recent years, along with the technology development of single molecule manipulation and detection, magnetic tweezer is current Application comparison a kind of single molecule manipulation device widely, it is that the two ends of experimental molecule are separately fixed on slide and a magnetic ball, then an additional magnetic field controls magnetic ball, thus stretches to experimental molecule or reverse.By the change of magnetic ball position, the length that can calculate experimental molecule, the power be subject to and moment of torsion.Although prior art can carry out fluorescence signal detection to sample and mechanics is handled, but measurement of the prior art is the view data of the same visual field, can not observe power spectrum and the spectroscopy procedure of molecule simultaneously, and can not obtain the image observation data of multi-angle, precision is not high.
Based on this, need badly at present a kind of can carry out fluorescence signal detection to sample molecule while, also can carry out mechanics manipulation to sample molecule, and improve the magnetic tweezer imaging system of measuring accuracy.
Summary of the invention
For prior art Problems existing, embodiments provide a kind of two imaging magnetic tweezers system, while solving and can not carrying out fluorescence signal detection to sample molecule in prior art, also can carry out the technical matters of mechanics manipulation to sample molecule.
The invention provides a kind of two imaging magnetic tweezers system, described system comprises:
Bogey, described bogey is for carrying sample molecule;
Magnetic tweezers, described magnetic tweezers is used for carrying out mechanics manipulation to described sample molecule, moves to make described sample molecule;
Micro-measure and control device, described micro-measure and control device is used for the first view data gathering the motion of described sample molecule from the side of described sample molecule;
Microscopic imaging device, described microscopic imaging device is used for the second view data gathering the motion of described sample molecule from the top of described sample molecule;
Control device, described control device is used for analyzing described first view data, obtains the first analysis result; Described second view data is analyzed, obtains the second analysis result; Verify described first analysis result and described second analysis result, determine that described sample molecule is in three dimensions result over time.
In such scheme, described bogey comprises:
Fluid pump, described fluid pump is used for extracting described sample molecule, makes described sample molecule enter described sample cell;
Sample cell, described sample cell is connected with one end of described sample molecule;
Objective table, described objective table is for supporting described sample cell;
Magnetic ball, described magnetic ball is connected with the other end of described sample molecule.
In such scheme, described magnetic tweezers comprises: magnet, vertical motor and rotation motor; Wherein,
Described vertical motor vertically moves up and down for controlling described magnet, to change the power be applied on described sample molecule;
Described rotation motor rotates in the horizontal direction for controlling described magnet, to control the number of revolutions of described sample molecule.
In such scheme, described micro-measure and control device comprises: the first light source, prism, the first spectroscope, the first object lens and the first video camera; Wherein,
Described first light source for generation of excitation beam, for described first object lens provide illumination;
Described prism and described first spectroscope are used for making described excitation beam irradiate described sample molecule, with transmitted ray;
First object lens, described first object lens are used for carrying out optical imagery to described sample molecule, obtain the first view data;
First video camera, described first digital signal is sent to described control device after being used for that described first view data is converted into the first digital signal by described first video camera.
In such scheme, described microscopic imaging device comprises: secondary light source, the second spectroscope, voltage amplifier, piezoelectric ceramics, the second object lens and the second video camera; Wherein,
Described secondary light source for generation of incident ray, for described second object lens provide illumination;
Described second spectroscope is used for reflecting described incident ray, with emergent ray;
Described voltage amplifier is used for making described piezoelectric ceramics produce deformation by changing voltage;
The deformation that described second object lens are used for producing according to described piezoelectric ceramics is moved, and obtains described sample molecule moves generation the second view data along optical axis;
Described second digital signal is sent to described control device after being used for that described second view data is converted into the second digital signal by described second video camera.
In such scheme, described control device comprises: piezo-electric drive units, magnetic tweezer control module and graphics processing unit;
Described piezo-electric drive units produces voltage for controlling described voltage amplifier;
Described magnetic tweezer control module vertically moves up and down for controlling vertical motor, controls rotation motor and rotates in the horizontal direction;
Described graphics processing unit is used for carrying out except analysis of making an uproar to described first digital signal and described second digital signal, obtains the analysis result of described sample molecule different angles image.
In such scheme, described prism is 45 ° of prisms.
In such scheme, described second object lens are oil immersion objective.
The invention provides a kind of two imaging magnetic tweezers system, described system comprises: bogey, and described bogey is for carrying sample molecule; Magnetic tweezers, described magnetic tweezers is used for carrying out mechanics manipulation to described sample molecule, moves to make described sample molecule; Micro-measure and control device, described micro-measure and control device is used for the first view data gathering the motion of described sample molecule from the side of described sample molecule; Microscopic imaging device, described microscopic imaging device is used for the second view data gathering the motion of described sample molecule from the top of described sample molecule; Control device, described control device is used for analyzing described first view data, obtains the first analysis result; Described second view data is analyzed, obtains the second analysis result; Verify described first analysis result and described second analysis result, determine that described sample molecule is in three dimensions result over time; So, while fluorescence signal detection can being carried out by micro-measure and control device to sample molecule, mechanics manipulation can also be carried out by microscopic imaging device to described sample molecule; And can verify according to the first view data got and the second view data, improve measuring accuracy.
Accompanying drawing explanation
Two imaging magnetic tweezers system one-piece construction block diagrams that Fig. 1 provides for the embodiment of the present invention one;
The one-piece construction schematic diagram of the bogey that Fig. 2 provides for the embodiment of the present invention one;
The one-piece construction schematic diagram of two imaging magnetic tweezers systems that Fig. 3 provides for the embodiment of the present invention one;
The one-piece construction schematic diagram of the control device that Fig. 4 provides for the embodiment of the present invention one;
λ-DNA molecular that Fig. 5 provides for the embodiment of the present invention two respectively with the connection diagram of magnetic ball and sample cell;
The vascular endothelial cell that Fig. 6 provides for the embodiment of the present invention three respectively with the connection diagram of magnetic ball and sample cell.
Embodiment
While can carrying out fluorescence signal detection to sample molecule, also can carry out mechanics manipulation to sample molecule, the invention provides a kind of two imaging magnetic tweezers system, described system comprises: bogey, and described bogey is for carrying sample molecule; Magnetic tweezers, described magnetic tweezers is used for carrying out mechanics manipulation to described sample molecule, moves to make described sample molecule; Micro-measure and control device, described micro-measure and control device is used for the first view data gathering the motion of described sample molecule from the side of described sample molecule; Microscopic imaging device, described microscopic imaging device is used for the second view data gathering the motion of described sample molecule from the top of described sample molecule; Control device, described control device is used for analyzing described first view data, obtains the first analysis result; Described second view data is analyzed, obtains the second analysis result; Verify described first analysis result and described second analysis result, determine that described sample molecule is in three dimensions result over time.
Below by drawings and the specific embodiments, technical scheme of the present invention is described in further detail.
Embodiment one
The present embodiment provides a kind of two imaging magnetic tweezers system, and as shown in Figure 1, described system comprises: bogey 1, magnetic tweezers 2, micro-measure and control device 3, microscopic imaging device 4 and control device 5; Wherein,
Described bogey 1 is for carrying sample molecule, so that described system is tested described sample molecule.
Particularly, as shown in Figure 2, described bogey 1 comprises: fluid pump 21, sample cell 22, objective table 23 and magnetic ball 24; Wherein,
When needs are tested sample molecule, the other end of described sample molecule is connected with described magnetic ball 24 in damping fluid, the sample feeding pipe 25 of described damping fluid with described sample cell 22 is connected, by described fluid pump 21, described sample molecule is extracted, make described sample molecule enter described sample cell 22 along sample feeding pipe 25; After sample molecule is full of sample cell 22, close fluid pump 21, and described sample cell 22 is inverted a period of time, to make one end of described sample molecule be connected with described magnetic ball 24, the other end is connected with the upper surface of sample cell 22.Described magnetic ball 24 is superparamagnetism bead.Described objective table 23 is for supporting described sample cell 22.Here, after being completed described sample molecule, described sample molecule can flow out by going out sample pipe 26.
After described sample molecule sets, described magnetic tweezers 2 specifically for: mechanics manipulation is carried out to described sample molecule, moves to make described sample molecule.
Particularly, described magnetic tweezers 2 is arranged on the below of described bogey 1, and as shown in Figure 3, described magnetic tweezers 2 comprises: magnet 31, vertical motor 32 and rotation motor 33; Wherein,
Described vertical motor 32 vertically moves up and down for controlling described magnet 31, and moving up and down of magnet 31 causes the motion of described magnetic ball 24, is applied to the power on described sample molecule by the position and then change changing magnet 31 in the vertical direction; Described rotation motor 33 rotates in the horizontal direction for controlling described magnet 31, controls the number of revolutions of described sample molecule, horizontally rotates with what observe magnet 31 the movement causing magnetic ball 24 along optical axis.
Further, described micro-measure and control device 3 is arranged on the side of described sample cell 22, relative translation can be done with sample cell 22 in the vertical direction, for gathering the first view data of described sample molecule motion from the side of described sample molecule, described first view data is converted into the first digital signal.
Particularly, as shown in Figure 3, described micro-measure and control device 3 comprises: the first light source 34, prism 35, first spectroscope 36, first object lens 37 and the first video camera 38; Wherein,
Described first light source 34 for generation of excitation beam, for described first object lens 37 provide illumination; Described prism 35 and described first spectroscope 36 irradiate described sample molecule for making described excitation beam, and described sample molecule is emitted beam, and described light reflects through described prism 35 and described first spectroscope 36 again, are transmitted on described first object lens 37; Wherein, described prism 35 is 45 ° of prisms.
Described first object lens 37, for carrying out optical imagery according to described light to described sample molecule, obtain the first view data; Wherein, described sample molecule can be not with fluorescently-labeled biomolecule, also can be the biomolecule be combined with fluorescently-labeled protein molecule.
After described first object lens 37 get described first view data, described first video camera 38 is for gathering described first view data, and described first view data is converted into the first digital signal, described first digital signal is sent to described control device 5.
After described control device 5 receives described first digital signal, described first digital signal is analyzed, by the assorted data of making an uproar of the first digital signal described in medium filtering or Mean Filtering Algorithm filtering, obtain the first analysis result.Described first analysis result comprises: the length of described sample molecule and structure change.
Further, described microscopic imaging device 4 is arranged on the top of described sample cell 22, relative translation can be done in the horizontal direction with sample cell 22, for gathering the second view data of described sample molecule motion from the top of described sample molecule, described second view data is converted into the second digital signal.
Particularly, as shown in Figure 3, described microscopic imaging device 4 comprises: secondary light source 41, second spectroscope 42, voltage amplifier 43, piezoelectric ceramics 44, second object lens 45 and the second video camera 46; Wherein,
Described secondary light source 41 for generation of incident ray, for described second object lens provide illumination; Described second spectroscope 42, for reflecting described incident ray, makes incident ray expose to described sample molecule, and emergent ray to the second video camera 46.
Described voltage amplifier 43 is connected with the side of described piezoelectric ceramics 44, for making described piezoelectric ceramics 44 produce deformation by changing the voltage be applied on described piezoelectric ceramics 44.
Described second object lens 45 are connected with one end of described piezoelectric ceramics 44, deformation for producing according to described piezoelectric ceramics 44 is moved, thus adjust described second object lens 45 focal length, and then follow the tracks of the movement of magnetic ball 24 along its optical axis, to obtain described sample molecule to move generation the second view data along optical axis; Wherein, described second object lens 45 are oil immersion objective.
When described second object lens 45 get the second view data, described second view data for gathering described second view data, and is converted into the second digital signal by described second video camera 46, and described second digital signal is sent to described control device 5.
After described control device 5 receives the second digital signal, described control device 5, also for analyzing described second view data, by the assorted data of making an uproar of the second digital signal described in medium filtering or Mean Filtering Algorithm filtering, obtains the second analysis result; Described second analysis result comprises: the length of described sample molecule and structure change.
Particularly, as shown in Figure 4, described control device 5 comprises: piezo-electric drive units 51, magnetic tweezer control module 52 and graphics processing unit 53; Wherein,
Described piezo-electric drive units 51 produces voltage for controlling described voltage amplifier 43, is applied to by described voltage on described piezoelectric ceramics 44; Described magnetic tweezer control module 52 vertically moves up and down for controlling vertical motor 32, vertically moves up and down to control described magnet 31; Control rotation motor 33 to rotate in the horizontal direction, rotate in the horizontal direction with described magnet 31, and then control the number of revolutions of described sample molecule.
Described graphics processing unit 53, for carrying out except analysis of making an uproar to described first digital signal and described second digital signal, obtains the analysis result of described sample molecule different angles image.
Such as, when described sample molecule is when not being with fluorescently-labeled biomolecule, described control device 5 calculates described magnetic ball 24 relative position information according to described second digital signal, and length and the structure of extrapolating the described sample molecule be connected with described magnetic ball 24 change.When described sample molecule is with fluorescently-labeled biomolecule, the fluorescence signal that described control device 5 is recorded by described first video camera 38, directly can record the length of described sample molecule, also directly can observe described sample molecule and change with the structure with fluorescent marker protein interaction of molecules.
After described control device 5 determines the first analysis result and the second analysis result, described first analysis result and described second analysis result can be verified, determine that described sample molecule is in three dimensions result over time.
In practical application, described control device 5 can be realized by computing machine; Described piezo-electric drive units 51, magnetic tweezer control module 52 and graphics processing unit 53 can by the central processing unit (CPU in described control device 5, Central Processing Unit), digital signal processor (DSP, Digtal SignalProcessor), programmable logic array (FPGA, Field Programmable Gate Array), micro-control unit (MCU, Micro Controller Unit) realize.
Two imaging magnetic tweezers systems that the present embodiment provides, double light path is utilized to observe biomolecule or cell sample in level and vertical both direction, while fluorescence signal detection can being carried out to sample molecule, also mechanics manipulation can be carried out to sample molecule, and the 26S Proteasome Structure and Function information obtained can be verified mutually, reflect that sample molecule is in three dimensions result over time, not only increase measuring accuracy, its result also has more cogency.In addition, described system has the advantage of micro-nano-scale power spectrum and spectrum concurrently, the length of individual cells or biomolecule and stressed size both can be detected intuitively, also can observe their image.
Embodiment two
During practical application, when described sample molecule is λ-DNA molecular, described λ-DNA one end, with biotin labeling, can have the magnetic ball 24 of Streptavidin albumen to be connected with pan coating; Described λ-DNA the other end, with marking sulfhydryl, can be connected with sample cell 22 surface.Specific as follows to the testing process of described λ-DNA molecular:
First, the other end of described λ-DNA molecular is connected with described magnetic ball 24 in damping fluid, the sample feeding pipe 25 of described damping fluid with described sample cell 22 is connected, by described fluid pump 21, described λ-DNA molecular is extracted, make described λ-DNA molecular enter described sample cell 22 along sample feeding pipe 25; After λ-DNA molecular is full of sample cell 22, closes fluid pump 21, and described sample cell 22 is inverted a period of time, be connected with the upper surface of described sample cell 22 to make one end of described λ-DNA molecular.The diameter of described magnetic ball 24 is 2.8 μm.Like this, as shown in Figure 5, one end of described λ-DNA molecular connects magnetic ball 24, and the other end is connected to the upper surface of sample cell 22.
Secondly, adjustment magnetic tweezers 2, makes described magnetic tweezers 2 arrive the below of sample cell 22, controls vertical motor 32 by described control device 5, drive described magnet 31 vertically to move up and down, and then causes moving up and down of magnetic ball 24 in described sample cell 22; Control rotation motor 33, drive described magnet 31 to rotate in the horizontal direction, control the number of revolutions of described λ-DNA molecular, horizontally rotate with what observe magnet 31 the movement causing magnetic ball 24 along optical axis, realize handling the mechanics of λ-DNA molecular.
Then, by fluid pump 21 inject in sample cell 22 be combined with λ-DNA molecular have fluorescently-labeled protein molecule.Open the first light source 34 of micro-measure and control device 3, for described first object lens 37 provide illumination, optical imagery is carried out to described λ-DNA molecular, obtains the first view data; The fluorescence signal in described first view data is recorded by the first video camera 38; After first view data is converted into the first digital signal by the first video camera 38, described first digital signal is sent to described control device 5.Close the first light source 34 of micro-measure and control device 3.
Further, open the secondary light source 41 of microscopic imaging device 4, for described second object lens 45 provide illumination, utilize voltage amplifier 43 and piezoelectric ceramics 44 to adjust described second object lens 45 focal length, and then follow the tracks of the movement of magnetic ball 24 along its optical axis; Optical imagery is carried out to described λ-DNA molecular, obtains the second view data; After second view data is converted into the second digital signal by described second video camera 46, described second digital signal is sent to described control device 5.
Finally, after described control device 5 receives the first digital signal and the second digital signal, the length of described λ-DNA molecular directly can be recorded according to the fluorescence signal in described first digital signal, also can observing protein molecule and the interactional picture structure of λ-DNA molecular; Here, the length variations of described λ-DNA molecular changes with the structure in image is corresponding.
According to the relative position information of magnetic ball 24 in described second digital signal, length and the structure of reckoning and the connected λ-DNA molecular of magnetic ball 24 change.
Like this, from facing the view data obtaining described λ-DNA molecular with side-looking two angles respectively, can reflect that λ-DNA molecular is in three dimensions structure over time, thus the function of λ-DNA molecular can be disclosed better.
Embodiment three
Relative to embodiment two, the sample molecule in the present embodiment is vascular endothelial cell, and described vascular endothelial cell one end can be connected with the magnetic ball 24 being coated with fibronectin, and the other end can be connected with sample cell 22 surface.Specific as follows to the testing process of described vascular endothelial cell:
First, the other end of described vascular endothelial cell is connected with described magnetic ball 24 in damping fluid, the sample feeding pipe 25 of described damping fluid with described sample cell 22 is connected, by described fluid pump 21, described vascular endothelial cell is extracted, make described vascular endothelial cell enter described sample cell 22 along sample feeding pipe 25; After vascular endothelial cell is full of sample cell 22, closes fluid pump 21, and described sample cell 22 is inverted a period of time, be connected with the upper surface of described sample cell 22 to make one end of described vascular endothelial cell.The diameter of described magnetic ball 24 is 1 μm.Like this, as shown in Figure 6, one end of described vascular endothelial cell connects magnetic ball 24, and the other end is connected to the upper surface of sample cell 22.
Secondly, adjustment magnetic tweezers 2, makes described magnetic tweezers 2 arrive the below of sample cell 22, controls vertical motor 32 by described control device 5, drive described magnet 31 vertically to move up and down, and then causes moving up and down of magnetic ball 24 in described sample cell 22; Control rotation motor 33, drive described magnet 31 to rotate in the horizontal direction, control the number of revolutions of described vascular endothelial cell, horizontally rotate with what observe magnet 31 the movement causing magnetic ball 24 along optical axis, realize handling the mechanics of vascular endothelial cell.
Then, open the first light source 34 of micro-measure and control device 3, for described first object lens 37 provide illumination, optical imagery is carried out to described vascular endothelial cell, obtains the first view data; First view data is sent to described control device 5 by the first video camera 38, directly can observe the image of vascular endothelial cell on the display of described control device 5.Close the first light source 34 of micro-measure and control device 3.
Open the secondary light source 41 of microscopic imaging device 4, for described second object lens 45 provide illumination, voltage amplifier 43 and piezoelectric ceramics 44 is utilized to adjust described second object lens 45 focal length, and then follow the tracks of the movement of magnetic ball 24 along its optical axis, optical imagery is carried out to described vascular endothelial cell, obtains the second view data; After second view data is converted into the second digital signal by described second video camera 46, described second digital signal is sent to described control device 5.
Finally, after described control device 5 receives the second digital signal, can according to the relative position information of magnetic ball 24 in described second digital signal, the structures such as the length of reckoning and the connected vascular endothelial cell of magnetic ball 24 and viscoelasticity change.
Like this, from facing the view data obtaining described vascular endothelial cell with side-looking two angles respectively, can reflect that vascular endothelial cell is in three dimensions structure over time, thus the function of vascular endothelial cell can be disclosed better.
The above, be only preferred embodiment of the present invention, be not intended to limit protection scope of the present invention, and all any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.
Claims (8)
1. a two imaging magnetic tweezers system, it is characterized in that, described system comprises:
Bogey, described bogey is for carrying sample molecule;
Magnetic tweezers, described magnetic tweezers is used for carrying out mechanics manipulation to described sample molecule, moves to make described sample molecule;
Micro-measure and control device, described micro-measure and control device is used for the first view data gathering the motion of described sample molecule from the side of described sample molecule;
Microscopic imaging device, described microscopic imaging device is used for the second view data gathering the motion of described sample molecule from the top of described sample molecule;
Control device, described control device is used for analyzing described first view data, obtains the first analysis result; Described second view data is analyzed, obtains the second analysis result; Verify described first analysis result and described second analysis result, determine that described sample molecule is in three dimensions result over time.
2. the system as claimed in claim 1, is characterized in that, described bogey comprises:
Fluid pump, described fluid pump is used for extracting described sample molecule, makes described sample molecule enter described sample cell;
Sample cell, described sample cell is connected with one end of described sample molecule;
Objective table, described objective table is for supporting described sample cell;
Magnetic ball, described magnetic ball is connected with the other end of described sample molecule.
3. the system as claimed in claim 1, is characterized in that, described magnetic tweezers comprises: magnet, vertical motor and rotation motor; Wherein,
Described vertical motor vertically moves up and down for controlling described magnet, to change the power be applied on described sample molecule;
Described rotation motor rotates in the horizontal direction for controlling described magnet, to control the number of revolutions of described sample molecule.
4. the system as claimed in claim 1, is characterized in that, described micro-measure and control device comprises: the first light source, prism, the first spectroscope, the first object lens and the first video camera; Wherein,
Described first light source for generation of excitation beam, for described first object lens provide illumination;
Described prism and described first spectroscope are used for making described excitation beam irradiate described sample molecule, with transmitted ray;
First object lens, described first object lens are used for carrying out optical imagery to described sample molecule, obtain the first view data;
First video camera, described first digital signal is sent to described control device after being used for that described first view data is converted into the first digital signal by described first video camera.
5. system as claimed in claim 4, it is characterized in that, described microscopic imaging device comprises: secondary light source, the second spectroscope, voltage amplifier, piezoelectric ceramics, the second object lens and the second video camera; Wherein,
Described secondary light source for generation of incident ray, for described second object lens provide illumination;
Described second spectroscope is used for reflecting described incident ray, with emergent ray;
Described voltage amplifier is used for making described piezoelectric ceramics produce deformation by changing voltage;
The deformation that described second object lens are used for producing according to described piezoelectric ceramics is moved, and obtains described sample molecule moves generation the second view data along optical axis;
Described second digital signal is sent to described control device after being used for that described second view data is converted into the second digital signal by described second video camera.
6. system as claimed in claim 5, it is characterized in that, described control device comprises: piezo-electric drive units, magnetic tweezer control module and graphics processing unit;
Described piezo-electric drive units produces voltage for controlling described voltage amplifier;
Described magnetic tweezer control module vertically moves up and down for controlling vertical motor, controls rotation motor and rotates in the horizontal direction;
Described graphics processing unit is used for carrying out except analysis of making an uproar to described first digital signal and described second digital signal, obtains the analysis result of described sample molecule different angles image.
7. system as claimed in claim 4, it is characterized in that, described prism is 45 ° of prisms.
8. system as claimed in claim 5, it is characterized in that, described second object lens are oil immersion objective.
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| CN108645795A (en) * | 2018-04-28 | 2018-10-12 | 华南理工大学 | A kind of multichannel list albumen magnetic tweezer investigating method and system |
| CN110320186A (en) * | 2019-06-06 | 2019-10-11 | 华南理工大学 | A kind of multichannel flow cavity and magnetic tweezer complex detection system and method |
| CN110887825A (en) * | 2019-12-10 | 2020-03-17 | 上海交通大学 | Biomechanical parameter measuring method based on controllable magnetic field |
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