CN101701244A - Method for sorting and characterizing cells based on dynamic optical tweezers and system thereof - Google Patents
Method for sorting and characterizing cells based on dynamic optical tweezers and system thereof Download PDFInfo
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Abstract
The invention discloses a method for sorting and characterizing cells based on dynamic optical tweezers and a system thereof. The method comprises the steps: the light beam of the optical tweezers is operated to lead the cells to circularly move; the cells can escape when reaching the escape speed; and the cells with different refractive indexes can be sorted and characterized as the cells with the different refractive indexes have different escape speeds. The invention can distinguish the cells with different status, can be widely used for the technical field of medicine, and has important production practice meaning.
Description
Technical field
The present invention relates to optical technical field, particularly relate to a kind of method for sorting and characterizing cells and system thereof based on dynamic optical tweezers.
Background technology
Optical tweezer technology is as a kind of technology that moves small items by laser beam, at present with biomedical very general with combining of optical tweezer technology, some biological natures with physical property characterising biological cell also are directions of present technical development, and the cell change of refractive is a physical property of characterize cells internal structure change.Therefore the research of pair cell specific refractory power is a kind of physical method of auxiliary medical diagnosis.
The current method of utilizing cell change of refractive characterize cells state that has realized in test includes: one, light tweezer tensile cell method, the cell deformation degree difference of different refractivity; Two, the light tweezer pushes away cell, the cell speed difference of different refractivity, three, atomic force microscope is surveyed cell surface power, and allogenic cell specific refractory power difference means that the internal structure of cell changes, and therefore the method with cell change of refractive characterize cells state can combine with medical diagnosis.
Therefore, to utilizing the different pair cells of specific refractory power to carry out sorting and sign has very important medical and practical significance.And measure the cell specific refractory power with physical method, thereby be applied in the medical diagnosis, abandoned medically and will demarcate, not only can guarantee cell activity, and, therefore can not injure the characteristic of cell because pair cell is the noncontact manipulation with fluorescent dye.
Say that from physical angle light tweezer manipulation technology is quite ripe, the light tweezer is handled biomass cells, and it is very general to be applied to little manipulation, and the light tweezer is applied to sorting and characterizing cells good technical foundation.
Summary of the invention
In view of this, the purpose of this invention is to provide a kind of method for sorting and characterizing cells and system thereof based on dynamic optical tweezers, can carry out circumferential motion with light tweezer optical beam steering cell, cell escaping phenomena can occur when reaching escape velocity, in view of the cell of different refractivity has different escape velocitys, thereby sorting characterizes the cell of different refractivity, realizes the cell of different states is distinguished, can be widely used in medicine technology field, be of great practical significance.
For this reason, the invention provides a kind of method for sorting and characterizing cells, may further comprise the steps based on dynamic optical tweezers:
Utilize light tweezer light beam that cell to be detected is shone, realize with light tweezer beam-capturing cell to be detected;
Rotation light tweezer light beam carries out circumferential motion, and cell to be detected is along with light tweezer light beam carries out circumferential motion;
Progressively improve to regulate the speed of rotation of light tweezer light beam, when escaping phenomena appears in each cell to be detected, speed of rotation of real time record light tweezer light beam and gather the image that this escaping phenomena cell occurs accordingly simultaneously all;
The size of the light tweezer light beam speed of rotation that when escaping phenomena occurring, has according to all cells of above-mentioned record, all cells are classified, and output shows the cell to appearance escaping phenomena that should light tweezer light beam speed of rotation, and final the realization characterizes the sorting of different cells.
Preferably, be used for the refractive sheet glass, rotate this sheet glass, make light tweezer light beam deviation occur and realize circumferential motion by light tweezer light beam is passed.
Preferably, the described refractive sheet glass that is used for is rotated motion in the vertical.
In addition, the present invention also provides a kind of sorting and characterizing cells system based on dynamic optical tweezers, includes:
Laser apparatus 1 is used to send light tweezer laser beam and level and shines telescopic system 2;
Telescopic system 2, this telescopic system 2 is made of a pair of lens, is positioned at the left side of described laser apparatus 1, and the horizontal laser light that laser apparatus 1 sends shines on the plane mirror 4 after passing this telescopic system 2;
Dynamic swivel arrangement, be used to drive the refractive sheet glass of installing on it 3 that is used for and be rotated motion, make and to carry out circumferential motion through the light tweezer light beam deviation of this sheet glass 3, the sheet glass of installing on the described dynamic swivel arrangement 3 is between a pair of lens that described telescopic system 2 comprises;
Plane mirror 4, described plane mirror 4 is positioned at the left side of described telescopic system 2, described plane mirror 4 comprises last plane mirror 41 and the lower plane mirror 42 that vertically distributes up and down, described upward plane mirror 41 and lower plane mirror 42 along continuous straight runs are symmetry up and down, and the angle of described lower plane mirror 42 and horizontal plane is that the head of acute angle and this lower plane mirror 42 is tilted to the left;
Beam splitting dichroic mirror 5 is positioned at described the right of going up plane mirror 41, and described upward plane mirror 41 is in the same plane with beam splitting dichroic mirror 5, and described upward plane mirror 41 and beam splitting dichroic mirror 5 be left-right symmetry vertically;
Be provided with powerful microscope 6 under the described beam splitting dichroic mirror 5, described powerful microscope 6 belows are provided with micro-cell sample chip 7, described micro-cell sample chip 7 is positioned on the mobile platform 9, be provided with lighting source 8 under the described micro-cell sample chip 7, described lighting source 8 is positioned at the below of mobile platform 9;
Be provided with ccd video camera 10 directly over the described beam splitting dichroic mirror 5, described ccd video camera 10 is connected with a computer 11.
Preferably, described laser apparatus 1 is the laser apparatus of 1064nm wavelength, and described telescopic system 2 is the anti-reflection lens of a pair of 1064nm wavelength, and described upward plane mirror 41 and lower plane mirror 42 are that 1064nm increases anti-plane mirror.
Preferably, described beam splitting dichroic mirror 5 is 45 degree beam splitting dichroic mirrors.
Preferably, described dynamic swivel arrangement drives its last sheet glass of installing and is rotated motion in the vertical.
Preferably, in the described dynamic swivel arrangement, described sheet glass 3 is installed in and drives on the brush, rotates the rotation that drives sheet glass by driving brush, and light beam has deviation and realizes circumferential motion in the light tweezer thereby make.
Preferably, described lower plane mirror 42 and dynamic swivel arrangement are installed above, and to be used for refractive sheet glass 3 in the same plane.
By above technical scheme provided by the invention as seen, the present invention proposes a kind of method for sorting and characterizing cells and system thereof based on dynamic optical tweezers, can carry out circumferential motion with light tweezer optical beam steering cell, cell escaping phenomena can occur when reaching escape velocity, in view of the cell of different refractivity has different escape velocitys, thereby sorting characterizes the cell of different refractivity, realization is distinguished the cell of different states, can be widely used in medicine technology field, be of great practical significance.
Description of drawings
Fig. 1 is the schema of a kind of method for sorting and characterizing cells based on dynamic optical tweezers provided by the invention;
Fig. 2 is the structural representation of a kind of sorting and characterizing cells system based on dynamic optical tweezers provided by the invention;
The sheet glass synoptic diagram that is used to produce beam deflection in the dynamic swivel arrangement of Fig. 3 for a kind of sorting and characterizing cells system based on dynamic optical tweezers provided by the invention;
Fig. 4 carries out the sample graph of circumferential motion for cell under sorting and characterizing cells system manipulation of the present invention.
Embodiment
In order to make those skilled in the art person understand the present invention program better, the present invention is described in further detail below in conjunction with drawings and embodiments.
Referring to Fig. 1, the invention provides a kind of method for sorting and characterizing cells based on dynamic optical tweezers, this method may further comprise the steps:
Step S101: utilize light tweezer light beam that cell to be detected is shone, realize with light tweezer beam-capturing cell to be detected;
Step S102: rotation light tweezer light beam carries out circumferential motion, and cell to be detected is along with light tweezer light beam carries out circumferential motion;
In the present invention, light tweezer light beam passed to be positioned at be used for the refractive sheet glass on the dynamic swivel arrangement, rotate this sheet glass, make light tweezer light beam deviation occur and realize circumferential motion.
Need to prove that on the specific implementation, the refractive sheet glass that is used for that dynamic swivel arrangement drives above it is rotated motion in the vertical;
In the present invention, on the specific implementation, by a dynamic swivel arrangement adjustable sheet glass is installed on the driving brush, brush rotates the rotation that drives sheet glass, make in the light tweezer light beam have certain deviation to realize circumferential motion, referring to Fig. 3, Fig. 3 is for being used to produce the sheet glass synoptic diagram of beam deflection in the dynamic swivel arrangement, in the drawings, the angle of light tweezer laser beam shown in the arrow and sheet glass is φ
i, exit angle is φ
r, the sheet glass inclination angle is θ, and the sheet glass specific refractory power is n, and the thickness of sheet glass is d.
Step S103: progressively improve to regulate the speed of rotation of light tweezer light beam, when escaping phenomena appears in each cell to be detected, speed of rotation of real time record light tweezer light beam and gather the image that this escaping phenomena cell occurs accordingly simultaneously all;
The above-mentioned escape velocity that is cell in the speed of rotation that escaping phenomena time tweezer light beam occurs.
Step S104: the size of the light tweezer light beam speed of rotation (speed of rotation that cell has) that when escaping phenomena occurring, has according to all cells of above-mentioned record, all cells are classified, and output shows the cell to appearance escaping phenomena that should light tweezer light beam speed of rotation, and final the realization characterizes the sorting of different cells.
Need to prove, the escape velocity that the material of different refractivity has is different, therefore escape velocity can be applied to the sign of specific refractory power, simultaneously, as previously mentioned, because different specific refractory poweres has characterized different cell states, therefore can classify to cell with different refractivity according to different escape velocitys, thereby sorting characterizes out the cell under the different states
Therefore the present invention, make circumferential motion by utilizing light tweezer optical beam steering cell to be detected, slowly regulate speed of rotation, when the speed of rotation of light tweezer light beam reaches cell escape velocity to be detected, write down the speed of rotation (equaling the escape velocity of cell to be detected) of this time tweezer light beam, different light tweezer light beam speed of rotation when the cell escaping phenomena occurring then, different cells are classified, thereby for allogenic cell not, or homocellular different states (changing over to if any gene) etc., the method that can use the invention described above to provide is carried out sorting.
A kind of method for sorting and characterizing cells based on dynamic optical tweezers based on the invention described above provides referring to Fig. 2, the invention provides a kind of sorting and characterizing cells system based on dynamic optical tweezers, and this system comprises:
Laser apparatus 1 is used to send light tweezer laser beam and level and shines telescopic system 2;
In the present invention, described laser apparatus 1 is preferably the laser apparatus of 1064nm wavelength;
Telescopic system 2, this telescopic system 2 is made of a pair of lens, is positioned at the left side of described laser apparatus 1, and the horizontal laser light that laser apparatus 1 sends shines on the plane mirror 4 after passing this telescopic system 2; In the present invention, the effect of described telescopic system 2 is in order to dwindle laser beam.
On the specific implementation, described telescopic system 2 is preferably the anti-reflection lens of a pair of 1064nm wavelength;
Dynamic swivel arrangement, be used to drive the refractive sheet glass of installing on it 3 that is used for and be rotated motion, make and to carry out circumferential motion through the light tweezer light beam deviation of this sheet glass 3, the sheet glass of installing on the described dynamic swivel arrangement 3 is between a pair of lens that described telescopic system 2 comprises;
Need to prove that on the specific implementation, dynamically swivel arrangement drives its last sheet glass of installing and is rotated motion in the vertical;
In the present invention, on the specific implementation, dynamically swivel arrangement is adjustable sheet glass to be installed in drive on the brush, rotate the rotation that drives sheet glass by driving brush, make in the light tweezer light beam have certain deviation to realize circumferential motion, referring to Fig. 3, Fig. 3 is for being used to produce the sheet glass synoptic diagram of beam deflection in the dynamic swivel arrangement, in the drawings, the angle of light tweezer laser beam shown in the arrow and sheet glass is φ
i, exit angle is φ
r, the sheet glass inclination angle is θ, and the sheet glass specific refractory power is n, and the thickness of sheet glass is d.
Described dynamic swivel arrangement voltage available is regulated speed of rotation, can be by the thickness of sheet glass, the device shape size of loading, and the inclination angle of sheet glass regulate rotation radius, other device is placed in this position also can realizes circumferential motion, as spatial light modulator, shake border etc.
Plane mirror 4, described plane mirror 4 is positioned at the left side of described telescopic system 2, described plane mirror 4 comprises that a pair of 1064nm that vertically distributes up and down increases anti-last plane mirror 41 and lower plane mirror 42, described upward plane mirror 41 and lower plane mirror 42 along continuous straight runs are symmetry up and down, and the angle of described lower plane mirror 42 and horizontal plane is that the head of acute angle and this lower plane mirror 42 is tilted to the left;
Need to prove that described lower plane mirror 42 and dynamic swivel arrangement are installed above, and to be used for refractive sheet glass 3 in the same plane.
Beam splitting dichroic mirror 5 is positioned at described the right of going up plane mirror 41, and described upward plane mirror 41 is in the same plane with beam splitting dichroic mirror 5, and described upward plane mirror 41 and beam splitting dichroic mirror 5 be left-right symmetry vertically;
In the present invention, described beam splitting dichroic mirror 5 is preferably 45 degree beam splitting dichroic mirrors.
Be provided with powerful microscope 6 under the described beam splitting dichroic mirror 5, described powerful microscope 6 belows are provided with micro-cell sample chip 7, described micro-cell sample chip 7 is positioned on the described three-dimensional fine adjustment mobile platform 9, be provided with lighting source 8 under the described micro-cell sample chip 7, described lighting source 8 is positioned at the below of three-dimensional fine adjustment mobile platform 9;
Be provided with CCD (charge coupled device) pick up camera 10 directly over the described beam splitting dichroic mirror 5, described ccd video camera 10 is connected with a computer 11.
The sorting and characterizing cells system that provides for the invention described above based on dynamic optical tweezers, in the concrete utilization, at first, the particle of cell to be detected and known specific refractory power sample is produced on the same micro-cell sample chip 7, then micro-cell sample chip 7 is placed on the three-dimensional fine adjustment mobile platform 9, and open laser apparatus 1, and output light tweezer laser beam, the output rating of light stable tweezer laser beam realizes the stable of target cell or sample particle caught; Determine the rotation radius of circumferential motion then, open the power supply of dynamic swivel arrangement, dynamically the energising voltage of swivel arrangement is regulated from low to high gradually very slowly, thereby realize allowing the sheet glass installed on the dynamic swivel arrangement carry out circumferential motion and speed of rotation slowly increases, as previously mentioned, light tweezer laser beam has certain deflection and carries out circumferential motion along with the rotation of sheet glass, light tweezer laser beam captured cell rotatablely moves along with light tweezer laser beam carries out circumference, referring to Fig. 4;
Among regulate process, by powerful microscope 6 and ccd video camera 10, the transient state of escape appears in computer 11 Real Time Observation that ccd video camera 10 is connected cell to be detected, and note the speed of rotation of dynamic swivel arrangement 3 correspondence when escaping phenomena appears in cell to be detected, laser output power;
Pair cell and sample particles are carried out aforesaid operations respectively then, gather the cell image to be detected that escaping phenomena occurs by computer 11, and while record data (being the speed of rotation of dynamic swivel arrangement), and repeat repeatedly to carry out, (is the speed of rotation of dynamic swivel arrangement with the cell image to be detected of the appearance escaping phenomena gathered with the data of record simultaneously, the escape velocity of cell to be detected just) analyzes, difference according to escape velocity, cell to be detected with different escape velocitys is classified, sub-elect the cell with different refractivity and show on computer 11, final sorting characterizes out the cell under the different states.
Need to prove, when the circumferential motion speed of light tweezer laser beam is lower than escape velocity, cell or sample particle are being followed light tweezer laser beam and are being rotated continuously together, when the circumferential motion speed of light tweezer laser beam reaches the escape velocity of cell, cell or sample particle moment from catch light beam (being in the light tweezer laser beam) break away from, and speed surpasses cell after the escape velocity or sample no longer with the motion campaign of light tweezer light beam.
The following describes principle of work of the present invention.
Cell in micro-cell sample chip 7 is subjected to the viscous resistance of surrounding environment solution, and the pulling force of light tweezer, viscous resistance constantly increases in the ever-increasing process of cell speed, there is a maximum value in the pulling force of light tweezer pair cell, in the ever-increasing process of cell speed, the speed of the corresponding maximum of light tweezer maximum pull when cell speed reaches top speed, escaping phenomena will occur.Specific refractory power is as a factor that influences pulling force, and the substance escaping speed of different refractivity is different, so the escape velocity of cell can be applied to the sign of cell specific refractory power.
Therefore the present invention, utilize manipulated cell to make circumferential motion, slowly regulate speed of rotation, when speed reaches escape velocity, record speed of rotation at this moment, not allogenic cell, or homocellular different states changes over to etc. if any gene, can use method and system provided by the invention to carry out sorting.
For the sorting and characterizing cells system based on dynamic optical tweezers provided by the invention, form by light tweezer and a dynamic swivel arrangement that produces circumferential motion, light tweezer laser beam drives by the cell of beam-capturing by this dynamic swivel arrangement and carries out circumferential motion, cell circumferential motion speed and radius can flexible, under the constant situation of radius, when speed is brought up to critical velocity, cell will break away from light tweezer light beam, escaping phenomena occur.Because the cell of different refractivity has different escape velocitys, and light tweezer reactive force refractive index fine difference is very responsive, and this system can realize the sorting and characterizing cells based on cell specific refractory power fine difference.
In the sorting and characterizing cells system based on dynamic optical tweezers provided by the invention, the cell of being handled by light tweezer laser beam is along with light beam carries out circumferential motion, movement velocity is transferred by the voltage control continuously differentiable, in the process that speed constantly slowly increases, escaping phenomena appears in cell when reaching certain speed, specific refractory power is the unique factor that influences escape velocity under the unified situation of other condition, if the specific refractory power difference, observed escape velocity is different in system.Therefore can realize that sorting characterizes by the cell refractive index difference, this system device is simple, the accuracy height.
Under the situation that dynamically the circumferential motion rotation radius of swivel arrangement is determined, can directly read by the voltage of control speed when reaching escape velocity, characterize speed with voltage.
In sum, compared with prior art, the present invention proposes a kind of method for sorting and characterizing cells and system thereof based on dynamic optical tweezers, can carry out circumferential motion with light tweezer optical beam steering cell, cell escaping phenomena can occur when reaching escape velocity, in view of the cell of different refractivity has different escape velocitys, thereby sorting characterizes the cell of different refractivity, realization is distinguished the cell of different states, can be widely used in medicine technology field, be of great practical significance.
Specific refractory power based on normal cell and cancer cells is different, method for sorting and characterizing cells and system thereof based on dynamic optical tweezers provided by the invention can be applied in the check of normal cell and cancer cells, replaces medical science whether a physical method of canceration takes place as cell.Normal cell in the early stage and cancer cells internal structure change but its size does not produce notable difference, corresponding its physical property is that its light refractive index changes, then use system provided by the invention, influence the escape velocity theory of cell circumferential motion by refractive index difference, pair cell investigates to see whether contain cancer cells.
The present invention can avoid the fluorescent dye in bio operon, and does not influence cell activity.
The above only is a preferred implementation of the present invention; should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the principle of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (9)
1. the method for sorting and characterizing cells based on dynamic optical tweezers is characterized in that, may further comprise the steps:
Utilize light tweezer light beam that cell to be detected is shone, realize with light tweezer beam-capturing cell to be detected;
Rotation light tweezer light beam carries out circumferential motion, and cell to be detected is along with light tweezer light beam carries out circumferential motion;
Progressively improve to regulate the speed of rotation of light tweezer light beam, when escaping phenomena appears in each cell to be detected, speed of rotation of real time record light tweezer light beam and gather the image that this escaping phenomena cell occurs accordingly simultaneously all;
The size of the light tweezer light beam speed of rotation that when escaping phenomena occurring, has according to all cells of above-mentioned record, all cells are classified, and output shows the cell to appearance escaping phenomena that should light tweezer light beam speed of rotation, and final the realization characterizes the sorting of different cells.
2. method for sorting and characterizing cells as claimed in claim 1 is characterized in that, is used for the refractive sheet glass by light tweezer light beam is passed, and rotates this sheet glass, makes light tweezer light beam deviation occur and realize circumferential motion.
3. method for sorting and characterizing cells as claimed in claim 2 is characterized in that, the described refractive sheet glass that is used for is rotated motion in the vertical.
4. the sorting and characterizing cells system based on dynamic optical tweezers is characterized in that, includes:
Laser apparatus (1) is used to send light tweezer laser beam and level and shines telescopic system (2);
Telescopic system (2), this telescopic system (2) is made of a pair of lens, is positioned at the left side of described laser apparatus (1), and the horizontal laser light that laser apparatus (1) sends shines on the plane mirror (4) after passing this telescopic system (2);
Dynamic swivel arrangement, be used to drive the refractive sheet glass of installing on it (3) that is used for and be rotated motion, make and to carry out circumferential motion that the sheet glass of installing on the described dynamic swivel arrangement (3) is positioned between a pair of lens that described telescopic system (2) comprises through the light tweezer light beam deviation of this sheet glass (3);
Plane mirror (4), described plane mirror (4) is positioned at the left side of described telescopic system (2), described plane mirror (4) comprises last plane mirror (41) and the lower plane mirror (42) that vertically distributes up and down, described upward plane mirror (41) and lower plane mirror (42) along continuous straight runs be symmetry up and down, and the angle of described lower plane mirror (42) and horizontal plane is that the head of acute angle and this lower plane mirror (42) is tilted to the left;
Beam splitting dichroic mirror (5) is positioned at described the right of going up plane mirror (41), and described upward plane mirror (41) is in the same plane with beam splitting dichroic mirror (5), and described upward plane mirror (41) and beam splitting dichroic mirror (5) be left-right symmetry vertically;
Be provided with powerful microscope (6) under the described beam splitting dichroic mirror (5), described powerful microscope (6) below is provided with micro-cell sample chip (7), described micro-cell sample chip (7) is positioned on the mobile platform (9), be provided with lighting source (8) under the described micro-cell sample chip (7), described lighting source (8) is positioned at the below of mobile platform (9);
Be provided with ccd video camera (10) directly over the described beam splitting dichroic mirror (5), described ccd video camera (10) is connected with a computer (11).
5. sorting and characterizing cells as claimed in claim 4 system, it is characterized in that, described laser apparatus (1) is the laser apparatus of 1064nm wavelength, and described telescopic system (2) is the anti-reflection lens of a pair of 1064nm wavelength, and described upward plane mirror (41) and lower plane mirror (42) are that 1064nm increases anti-plane mirror.
6. sorting and characterizing cells as claimed in claim 4 system is characterized in that, described beam splitting dichroic mirror (5) is 45 degree beam splitting dichroic mirrors.
7. sorting and characterizing cells as claimed in claim 4 system is characterized in that, described dynamic swivel arrangement drives its and goes up sheet glass of installing and be rotated motion in the vertical.
8. sorting and characterizing cells as claimed in claim 4 system, it is characterized in that in the described dynamic swivel arrangement, described sheet glass (3) is installed in and drives on the brush, rotate the rotation that drives sheet glass by driving brush, light beam has deviation and realizes circumferential motion in the light tweezer thereby make.
9. sorting and characterizing cells as claimed in claim 4 system is characterized in that, described lower plane mirror (42) and dynamic swivel arrangement are installed above, and to be used for refractive sheet glass (3) in the same plane.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102860845A (en) * | 2012-08-30 | 2013-01-09 | 中国科学技术大学 | Method and corresponding device for capturing and controlling in-vivo cells of living body animal |
| CN103547907A (en) * | 2012-03-30 | 2014-01-29 | 索尼公司 | Micro-particle isolation device and method for controlling position in the micro-particle isolation device |
| CN103676126A (en) * | 2013-12-20 | 2014-03-26 | 同济大学 | Operation instrument for optical tweezers |
| CN104215502A (en) * | 2014-03-17 | 2014-12-17 | 南方科技大学 | System and method for detecting cell elastic modulus |
| CN108703138A (en) * | 2018-07-08 | 2018-10-26 | 苏州美丽澄电子技术有限公司 | A kind of method and device freezed in optical tweezer cell and particle to liquid nitrogen |
| CN108918520A (en) * | 2018-07-09 | 2018-11-30 | 长沙健金电子技术有限公司 | A kind of devices and methods therefor of fish egg cell screening |
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2009
- 2009-11-03 CN CN 200910071098 patent/CN101701244A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103547907A (en) * | 2012-03-30 | 2014-01-29 | 索尼公司 | Micro-particle isolation device and method for controlling position in the micro-particle isolation device |
| CN102860845A (en) * | 2012-08-30 | 2013-01-09 | 中国科学技术大学 | Method and corresponding device for capturing and controlling in-vivo cells of living body animal |
| CN103676126A (en) * | 2013-12-20 | 2014-03-26 | 同济大学 | Operation instrument for optical tweezers |
| CN104215502A (en) * | 2014-03-17 | 2014-12-17 | 南方科技大学 | System and method for detecting cell elastic modulus |
| CN104215502B (en) * | 2014-03-17 | 2016-08-24 | 南方科技大学 | The detecting system of the elastic modelling quantity of cell and the detection method of the elastic modelling quantity of cell |
| CN108703138A (en) * | 2018-07-08 | 2018-10-26 | 苏州美丽澄电子技术有限公司 | A kind of method and device freezed in optical tweezer cell and particle to liquid nitrogen |
| CN108918520A (en) * | 2018-07-09 | 2018-11-30 | 长沙健金电子技术有限公司 | A kind of devices and methods therefor of fish egg cell screening |
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