CN108918520A - A kind of devices and methods therefor of fish egg cell screening - Google Patents
A kind of devices and methods therefor of fish egg cell screening Download PDFInfo
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- CN108918520A CN108918520A CN201810743023.XA CN201810743023A CN108918520A CN 108918520 A CN108918520 A CN 108918520A CN 201810743023 A CN201810743023 A CN 201810743023A CN 108918520 A CN108918520 A CN 108918520A
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- egg cell
- fish egg
- fish
- optical tweezer
- computer
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
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Abstract
The invention discloses a kind of fish egg cell screening plant, including fish-egg screening plant, the fish-egg screening plant includes electron microscope and the optical tweezer transmitter being arranged on electron microscope and sample room, it is characterised in that：The electron microscope includes pedestal, moving stage and lens barrel, imaging sensor is provided in the lens barrel, described image sensor, moving stage and optical tweezer transmitter are connect with computer, the computer is connect with display, the side of the lens barrel is provided with object lens, and the other side is provided with eyepiece.The logical fish egg cell that will cross of the present invention is placed on microscope, the survival rate and maturing rate of fish egg cell is checked using microscope, and capture underproof fish egg cell by optical tweezer.
Description
Technical field
The present invention relates to the devices and methods therefors that biological cell field more particularly to a kind of fish egg cell are screened.
Background technique
During fish-egg artificial insemination, milter sperm is mixed, and stirred with feather with fish-egg, connect smart ovum sufficiently
It is put into incubation tank and is hatched after touch.In hatching process, the maturity of fish-egg is the key that influence hatching rate height.But often
Method is the color of fish-egg to be observed by artificial eye, therefore can not accurately carry out fish-egg screening.
With the development of science and technology, the mankind are more and more frequent to the manipulation of microcosmos, therefore, to the need of such tool
Ask also growing day by day, and laser tweezers can be used to manipulate nano particle as a kind of tool being wherein widely adopted
(Nanoparticle), nano wire (Nanowire), nanotube (Nanotube), large biological molecule and cell etc., make the mankind more
Add and understand microcosmic dynamic process and interaction, and carries out arrangement as needed to microcosmic research object.Thin
In born of the same parents and biomolecule research, biologist wants to see the effect of molecule and cell, the effect of cell and cell, molecule
With the effect of molecule, these processes can be observed by optical optical tweezers system, or even measure phase in these interaction processes
The real-time change of interreaction force, to understand entire mechanism.Using microscopically observation biological cell or biology point
The period of the day from 11 p.m. to 1 a.m, due to the variation of temperature, cell or molecule can run out of the microscopical visual field, thus operator frequently to adjust it is aobvious
The visual field of micro mirror, but if it will vise biological cell or molecule, make to operate using optical tweezer come immobilizing biological samples
Person can preferably observe cell and molecule, and will reduce environmental change bring in this way influences, and keep observed result relatively reliable.
Therefore, those skilled in the art are dedicated to developing a kind of device that can accurately identify screening fish-egg.
Summary of the invention
It is an object of the invention to overcome problem above of the existing technology, a kind of device of fish egg cell screening is provided
And its method, the logical fish egg cell that will cross of the present invention are placed on microscope, using microscope check fish egg cell survival rate and
Maturing rate, and underproof fish egg cell is captured by optical tweezer.
To realize above-mentioned technical purpose and the technique effect, the invention is realized by the following technical scheme：
A kind of fish egg cell screening plant, including fish-egg screening plant, the fish-egg screening plant include electron microscope and
Optical tweezer transmitter and sample room on electron microscope are set, and the electron microscope includes pedestal, moving stage and mirror
Tin, imaging sensor is provided in the lens barrel, described image sensor, moving stage and optical tweezer transmitter and computer connect
It connects, the computer is connect with display, and the side of the lens barrel is provided with object lens, and the other side is provided with eyepiece.
Preferably, the optical tweezer transmitter includes the laser set gradually, prefocus cup, concave and convex lenses and optical fiber, it is described
The laser of laser transmitting is gathered into optical tweezer for clamping in sample room by prefocus cup, concave and convex lenses and optical fiber in sample room
Fish egg cell.
Preferably, the optical tweezer transmitter is using the transmitting of the optical tweezer of tunable laser source or adjustable intensity laser light source
Device.
Preferably, the sample room includes sample storage chamber and sample collection room, the sample collection room and sample are deposited
It puts and is provided with several fish egg cell channels between room.
Preferably, CCD camera or display monitor central monitoring system or video recorder are provided in the lens barrel, the CCD camera or prison
Show that device or video recorder are connect with computer.
Preferably, the computer includes cabinet and the main control board being arranged in cabinet and is arranged in master control
Processor and memory on circuit board.
A kind of method of fish egg cell screening, the method for the fish egg cell screening include the following steps：
1) fish egg cell screened will be needed to be placed in sample storage chamber；
2) fish egg cell abnormal in sample storage chamber is identified by imaging sensor by computer；
3) moving stage is controlled by computer, the abnormal fish egg cell on moving stage is made to be moved to optical tweezer transmitter institute
In the optical tweezer of generation；
4) laser emitted by computer adjustment optical tweezer transmitter is strong and weak, the fish egg cell for catching the exception；
5) moving stage is controlled by computer, the abnormal cell of capture is transferred to sample collection room.
The beneficial effects of the invention are as follows：
1. the logical fish egg cell that will cross of the present invention is placed on microscope, using microscope check fish egg cell survival rate and at
Ripe rate, and underproof fish egg cell is captured by optical tweezer；
2. greatly reducing workload, fish egg cell capture rate is greatly improved；The present invention automatic identification and can filter out
Mature fish-egg, to be greatly improved the survival rate of fish egg cell；
3. intuitive, sensitive, high frequency zone fish egg cell；
4. improving cell survival rate, help to separate underproof fish egg cell.
The above description is only an overview of the technical scheme of the present invention, in order to better understand the technical means of the present invention,
And can be implemented in accordance with the contents of the specification, with presently preferred embodiments of the present invention and attached drawing is cooperated to be described in detail below.This hair
Bright specific embodiment is shown in detail by following embodiment and its attached drawing.
Detailed description of the invention
The drawings described herein are used to provide a further understanding of the present invention, constitutes part of this application, this hair
Bright illustrative embodiments and their description are used to explain the present invention, and are not constituted improper limitations of the present invention.In the accompanying drawings：
Fig. 1 is structural schematic diagram of the present invention；
Fig. 2 is schematic diagram of the present invention；
Fig. 3 is 3 structural schematic diagram of sample room of the present invention.
Figure label explanation：Electron microscope 1, pedestal 101, moving stage 102, lens barrel 103, object lens 104, eyepiece
105, optical tweezer transmitter 2, sample room 3, imaging sensor 4, computer 5, display 6, fish egg cell 7, laser 201, optically focused
Cup 202, concave and convex lenses 203, optical fiber 204, sample storage chamber 301, sample collection room 302, fish egg cell channel 303, abnormal fish-egg
Cell 8, deflecting plate 9.
Specific embodiment
The invention will be further described with reference to the accompanying drawing：
Referring to figs. 1 to shown in Fig. 3, a kind of fish egg cell screening plant, including fish-egg screening plant, the fish-egg screening plant packet
Include electron microscope 1 and the optical tweezer transmitter 2 being arranged on electron microscope 1 and sample room 3, the electron microscope 1 wraps
Pedestal 101, moving stage 102 and lens barrel 103 are included, imaging sensor 4, described image sensing are provided in the lens barrel 103
Device 4, moving stage 102 and optical tweezer transmitter 2 are connect with computer 5, and the computer 5 is connect with display 6, the lens barrel
103 side is provided with object lens 104, and the other side is provided with eyepiece 105.
Preferably, the optical tweezer transmitter 2 includes laser 201, prefocus cup 202,203 and of concave and convex lenses set gradually
Optical fiber 204, the laser that the laser 201 emits gather in sample room 3 by prefocus cup 202, concave and convex lenses 203 and optical fiber 204
Integrated optical tweezer is used to clamp the fish egg cell 7 in sample room 3.
Preferably, the optical tweezer transmitter 2 is using the optical tweezer of tunable laser source or adjustable intensity laser light source hair
Emitter 2.
Preferably, the sample room 3 includes sample storage chamber 301 and sample collection room 302, the sample collection room
Several fish egg cell channels 303 are provided between 302 and sample storage chamber 301.
Preferably, be provided with CCD camera or display monitor central monitoring system or video recorder in the lens barrel 103, the CCD camera or
Display monitor central monitoring system or video recorder are connect with computer 5.
Preferably, the computer 5 includes cabinet and the main control board being arranged in cabinet and is arranged in master
Control the processor and memory on circuit board.
A kind of method of fish egg cell screening, the method for the fish egg cell screening include the following steps：
1) fish egg cell 7 screened will be needed to be placed in sample storage chamber 301；
2) fish egg cell 8 abnormal in sample storage chamber 301 is identified by imaging sensor 4 by computer 5；
3) moving stage 102 is controlled by computer 5, the abnormal fish egg cell 8 on moving stage 102 is made to be moved to optical tweezer hair
In optical tweezer caused by emitter 2；
4) fish egg cell 8 that the laser that optical tweezer transmitter 2 is emitted is strong and weak, for catching the exception is adjusted by computer 5；
5) moving stage 102 is controlled by computer 5, the abnormal cell 8 of capture is transferred to sample collection room 302.
Specific embodiment：
In actual use, the fish egg cell screened will be needed to be placed in sample storage chamber；Image sensing is passed through by computer
Device identifies fish egg cell abnormal in sample storage chamber；Moving stage is controlled by computer, makes the exception on moving stage
Fish egg cell is moved in optical tweezer caused by optical tweezer transmitter；The laser emitted by computer adjustment optical tweezer transmitter is strong
Weak, for catching the exception fish egg cell；Moving stage is controlled by computer, the abnormal cell of capture is transferred to sample and is received
Collect room.
The above description is merely a specific embodiment, but scope of protection of the present invention is not limited thereto, any
Those familiar with the art in the technical scope disclosed by the present invention, can easily think of the change or the replacement, and should all contain
Lid is within protection scope of the present invention.Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (7)
1. a kind of fish egg cell screening plant, including fish-egg screening plant, the fish-egg screening plant includes electron microscope（1）
And it is arranged in electron microscope（1）On optical tweezer transmitter（2）The sample room and（3）, it is characterised in that：The electron microscope
（1）Including pedestal（101）, moving stage（102）And lens barrel（103）, the lens barrel（103）Inside it is provided with imaging sensor
（4）, described image sensor（4）, moving stage（102）With optical tweezer transmitter（2）With computer（5）Connection, the calculating
Machine（5）With display（6）Connection, the lens barrel（103）Side be provided with object lens（104）, the other side is provided with eyepiece
（105）.
2. a kind of fish egg cell screening plant according to claim 1, it is characterised in that：The optical tweezer transmitter（2）Packet
Include the laser set gradually（201）, prefocus cup（202）, concave and convex lenses（203）And optical fiber（204）, the laser（201）Hair
The laser penetrated passes through prefocus cup（202）, concave and convex lenses（203）And optical fiber（204）In sample room（3）In be gathered into optical tweezer for clamping
Sample room（3）In fish egg cell（7）.
3. a kind of fish egg cell screening plant according to claim 1, it is characterised in that：The optical tweezer transmitter（2）It adopts
With tunable laser source or the optical tweezer transmitter of adjustable intensity laser light source（2）.
4. a kind of fish egg cell screening plant according to claim 1, it is characterised in that：The sample room（3）Including sample
Product storage chamber（301）And sample collection room（302）, the sample collection room（302）With sample storage chamber（301）Between be provided with
Several fish egg cell channels（303）.
5. a kind of fish egg cell screening plant according to claim 1, it is characterised in that：The lens barrel（103）Middle setting
There are CCD camera or display monitor central monitoring system or a video recorder, the CCD camera or display monitor central monitoring system or video recorder and computer（5）Connection.
6. a kind of fish egg cell screening plant according to claim 1, it is characterised in that：The computer（5）Including machine
Case and the main control board being arranged in cabinet and the processor and memory being arranged on main control board.
7. a kind of method of fish egg cell screening, which is characterized in that the method for the fish egg cell screening includes the following steps：
The fish egg cell that needs are screened（7）It is placed on sample storage chamber（301）In；
By computer（5）Pass through imaging sensor（4）Identify sample storage chamber（301）Middle abnormal fish egg cell（8）；
By computer（5）Control moving stage（102）, make moving stage（102）On abnormal fish egg cell（8）It is moved to
Optical tweezer transmitter（2）In generated optical tweezer；
By computer（5）Adjust optical tweezer transmitter（2）The laser emitted is strong and weak, the fish egg cell for catching the exception（8）；
By computer（5）Control moving stage（102）, by the abnormal cell of capture（8）It is transferred to sample collection room（302）.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810743023.XA CN108918520A (en) | 2018-07-09 | 2018-07-09 | A kind of devices and methods therefor of fish egg cell screening |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810743023.XA CN108918520A (en) | 2018-07-09 | 2018-07-09 | A kind of devices and methods therefor of fish egg cell screening |
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| Publication Number | Publication Date |
|---|---|
| CN108918520A true CN108918520A (en) | 2018-11-30 |
Family
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| CN201810743023.XA Withdrawn CN108918520A (en) | 2018-07-09 | 2018-07-09 | A kind of devices and methods therefor of fish egg cell screening |
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Citations (9)
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|---|---|---|---|---|
| CN1438479A (en) * | 2002-12-31 | 2003-08-27 | 上海中科大光镊科技有限公司 | Technology for sorting and extracting matter in liquid cell using laser |
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| CN101701244A (en) * | 2009-11-03 | 2010-05-05 | 南开大学 | Method for sorting and characterizing cells based on dynamic optical tweezers and system thereof |
| CN102181361A (en) * | 2011-03-25 | 2011-09-14 | 哈尔滨工业大学（威海） | Device and method for sorting cells |
| CN102792916A (en) * | 2011-05-25 | 2012-11-28 | 华中农业大学 | Method and device applicable to observing fish embryo development under stereo microscope |
| CN102860845A (en) * | 2012-08-30 | 2013-01-09 | 中国科学技术大学 | Method and corresponding device for capturing and controlling in-vivo cells of living body animal |
| CN103076283A (en) * | 2013-01-17 | 2013-05-01 | 中国水产科学研究院黑龙江水产研究所 | Method for observing developmental process of salmonidae roes |
| CN103674814A (en) * | 2012-09-13 | 2014-03-26 | 香港城市大学 | Methods and means for manipulating particles |
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2018
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Patent Citations (9)
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|---|---|---|---|---|
| CN1438479A (en) * | 2002-12-31 | 2003-08-27 | 上海中科大光镊科技有限公司 | Technology for sorting and extracting matter in liquid cell using laser |
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| CN101701244A (en) * | 2009-11-03 | 2010-05-05 | 南开大学 | Method for sorting and characterizing cells based on dynamic optical tweezers and system thereof |
| CN102181361A (en) * | 2011-03-25 | 2011-09-14 | 哈尔滨工业大学（威海） | Device and method for sorting cells |
| CN102792916A (en) * | 2011-05-25 | 2012-11-28 | 华中农业大学 | Method and device applicable to observing fish embryo development under stereo microscope |
| CN102860845A (en) * | 2012-08-30 | 2013-01-09 | 中国科学技术大学 | Method and corresponding device for capturing and controlling in-vivo cells of living body animal |
| CN103674814A (en) * | 2012-09-13 | 2014-03-26 | 香港城市大学 | Methods and means for manipulating particles |
| CN103076283A (en) * | 2013-01-17 | 2013-05-01 | 中国水产科学研究院黑龙江水产研究所 | Method for observing developmental process of salmonidae roes |
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Non-Patent Citations (1)
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Application publication date: 20181130 |