CN2758757Y - FS laser clamping device for trapping biological cells - Google Patents
FS laser clamping device for trapping biological cells Download PDFInfo
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- CN2758757Y CN2758757Y CN 200420085210 CN200420085210U CN2758757Y CN 2758757 Y CN2758757 Y CN 2758757Y CN 200420085210 CN200420085210 CN 200420085210 CN 200420085210 U CN200420085210 U CN 200420085210U CN 2758757 Y CN2758757 Y CN 2758757Y
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Abstract
The utility model discloses an FS laser clamping device for trapping biological cells, which belongs to the improvement on a light clamping device for trapping biological cells. The device comprises an FS pulse laser, a beam coupling device, a main body of an inverted microscope, and a monitoring system, wherein the repetition frequency of output pulse of the FS pulse laser is bigger than 70 megahertz; the pulse width is smaller than 100 FS; the beam coupling device comprises a convex lens, two plane mirrors and a rotary polarizing sheet; the main body of the inverted microscope is based on the IX71 type biologic inverted microscope of the Olympus company; the objective table is a two-dimension micrometric displacement platform; the control accuracy of a two-dimension linear excitation source is 0.2 micrometers; the monitoring system comprises an eye lens and a CCD camera connected with a computer. The utility model has the advantages the FS pulse laser with a high-repetition rate stably traps micron dimension particles and the biological cells; the accuracy can reach 0.2 micrometers; the utility model can monitor the operational process that the clamping device traps the cells for partial modification without damage at real time.
Description
Technical field
The utility model relates to a kind of femtosecond laser light forceps device of optical Trapping of Biological, belongs to the improvement of the light forceps device of optical Trapping of Biological.
Background technology
Cell is the simplest living unit, and the answer of all life problems finally all can be found in cell.In the life science system, the cell biology more tardy exhibition of why being born is also slow, one of reason no doubt is that cell itself is the life system of a complexity, and up to the present the more important thing is does not also have very gratifying technological means to be used for studying such life elementary cell.Contemporary optics, advanced laser technology and the development of technique of image display are for the research of cell biology provides high-tech means.The eighties mid-term, the Arthur Ashkin of U.S.'s Bell Laboratory finds that the Gaussian laser beam of focusing can be caught individual cells or protozoan, is developed into the manipulating cells that is widely used in and the instrument-laser tweezers of organelle afterwards.
At present, laser optical tweezer adopts continuous laser as light source more, and it and laser microbeam technique binding energy carry out operation such as cell perforation to biological cell.But determine the boundary of the various biological effects of the caused cell of irradiance, as damage threshold, toxic and side effect boundary, cause the laser work parameter of cell deactivation etc., being the difficult point with the research of continuous laser light tweezer, also is to restrict " bottleneck " that the operation of laser cell further develops at present.Cause the factor of this " bottleneck " a lot: one, the caused various biological effects of irradiance were both relevant with pulsewidth, energy and the wavelength of laser, acted on the influence of character of target own and physical environment thereof again; Its two, at present, be used for the laser instrument of laser capture microdissection operation pulsewidth broad (nanosecond), wavelength is single and poor stability, and single celled volume only flies liter (femtoliter, 10
-15Liter) magnitude, therefore more difficult Control work parameter; Its three, in the past, the research work focus of laser cell operation focused mostly on and was seeking on the new effective object.Therefore, research new pattern laser microscopic cells operation system, and as means, interaction mechanism between further investigation laser and the biological targets, get the toxic and side effect of laser pair cell in the fine operation of laser cell clear, and then definite optimum working parameter, become the key that overcomes " bottleneck ".Moreover, biological living cells is carried out micron and even the operation of the nothing of nano-resolution wound microbeam, as catch, the partial modification of perforation and pair cell device, the ultrafast bioprocess of research is the technological means that the biomedical sector expectation realizes always on unicellular level.
From the nineties in 20th century, femtosecond (1 femtosecond=10
-15Second) laser technology obtains tremendous development.The narrowest light impulse length is the 4-5 femtosecond at present, and the peak power of the femtosecond light pulse after the amplification is Da Taiwa (10
12Watt) more than.The extremely short pulse width of femto-second laser pulse and minimum single pulse energy just can obtain superpower peak power, make its with the Biomedia interaction process in show high time (femtosecond) and space (sub-micron) resolution.
Summary of the invention
The purpose of this utility model is to provide a kind of and realizes the stable device of catching of biological cell as light tweezer light source with the high-repetition-rate femtosecond pulse, this device is in the optical Trapping of Biological process, the nothing wound microbeam operation of micron and even nano-resolution can be carried out, and ultrafast bioprocess can be on unicellular level, studied.
The utility model is realized by following technical proposals, and a kind of femtosecond laser light forceps device of optical Trapping of Biological is characterized in that this device comprises femtosecond pulse laser 1, light beam coupling device 2, inverted microscope main body 3 and supervisory system composition.Described femtosecond pulse laser is a titanium-doped sapphire self mode locked fs laser generation level, and the output pulse repetition rate is greater than 70 megahertzes, and pulse width is less than 100 femtoseconds; Described beam coupler comprises that a focal length is 150 millimeters convex lens 2-1, two plane mirror 2-2,2-3 and rotatable polaroid 2-4, the built-in lens of convex lens and microscope form telescopic system, plane mirror is used to regulate the incident laser beam direction, its optical axis is overlapped fully with the optical axis of microscope imaging light path, can change by the rotatory polarization sheet and be incident to microscopical light pulse average power; Described inverted microscope main body 3 is based on the biological inverted microscope of the IX71 of Olympus company type, the objective table 3-3 of inverted microscope main body is the two-dimentional micro-displacement platform of two-dimensional linear driving source 4 controls, two-dimensional linear driving source 4 is subjected to computing machine 7 controls, and precision is 0.2 micron; The CCD camera 5 that described supervisory system comprises eyepiece 6 and links to each other with computing machine utilizes the illumination path 3-5 of inverted microscope main body, catches the process of cell by eyepiece or the monitoring in real time of CCD camera.
The utility model has the advantages that: can realize that the high-repetition-rate femtosecond pulse catches the stable of micron dimension particle and biological cell, its precision can reach 0.2 micron; Can monitor femtosecond laser light tweezer in real time and catch the process of cell, and be shown in computer screen, be convenient to data analysis; On device of the present utility model, binding time resolved spectroscopy technology can be studied the ultrafast bioprocess of unicellular organism body, and carries out the research of Fs laser double photon fluorescence photodynamics; And can make full use of the high time and the spatial discrimination characteristic of femtosecond laser, target cell is done not have the operation of wound partial modification.
Description of drawings
Fig. 1 is the structured flowchart of the utility model device.
Among the figure: 1-femtosecond pulse laser, 2-light beam coupling device, 2-1-convex lens, 2-2,2-3-plane mirror, 2-4-polaroid, 3-inverted microscope main body, the inner light path of 3-1-, 3-2-object lens, 3-3-objective table, the 3-4-sample box, the 3-5-illumination path, 4-two-dimensional linear driving source, 5-CCD camera, the 6-eyepiece, the 7-computing machine.
Embodiment
The utility model will be further described below in conjunction with accompanying drawing.
Referring to Fig. 1, the present invention adopts titanium-doped sapphire femtosecond pulse laser 1 as light tweezer light source, adopts the main body of inverted microscope 3 as apparatus of the present invention.From the light pulse of femtosecond pulse laser output is the line polarisation, and its repetition frequency is greater than 70 megahertzes, and pulse width is less than 100 femtoseconds.Utilize light beam coupling device 2, femtosecond pulse is coupled in the microscope.Can continuously change the average power that is coupled to microscopical femtosecond pulse by the rotatory polarization sheet.In microscope, femtosecond pulse and the reverse propagation of inverted microscope imaging optical path tightly focus on through the object lens 3-2 of high power, are converged to radius less than 1 micron hot spot, form the optics potential well.Utilize two-dimensional linear driving source 4 to control the target cell (erythrocyte) among the sample box 3-4 on the objective table 3-3 accurately, in the optics potential well with its shift-in femtosecond laser, realize the stable of target cell caught.The numerical aperture of the object lens 3-2 of high power is greater than 0.75.By means of the illumination path 3-5 of inverted microscope main body, can monitor in eyepiece 6 that femtosecond laser light tweezer catches the process of cell; Show and the recording operation process by CCD camera 5 and computing machine 7.
Claims (1)
1, a kind of femtosecond laser light forceps device of optical Trapping of Biological is characterized in that: this device comprises femtosecond pulse laser (1), light beam coupling device (2), inverted microscope main body (3) and supervisory system composition; Described femtosecond pulse laser is a titanium-doped sapphire self mode locked fs laser generation level, and the output pulse repetition rate is greater than 70 megahertzes, and pulse width is less than 100 femtoseconds; Described beam coupler comprises that a focal length is 150 millimeters convex lens (2-1), two plane mirrors (2-2), (2-3) and a rotatable polaroid (2-4), the built-in lens of convex lens and microscope form telescopic system, plane mirror is used to regulate the incident laser beam direction, its optical axis is overlapped fully with the optical axis of microscope imaging light path, can change by the rotatory polarization sheet and be incident to microscopical light pulse average power; Described inverted microscope main body (3) is based on the biological inverted microscope of the IX71 of Olympus company type, the objective table of inverted microscope main body (3-3) is the two-dimentional micro-displacement platform of two-dimensional linear driving source (4) control, the two-dimensional linear driving source is subjected to computing machine (7) control, and precision is 0.2 micron; The CCD camera (5) that described supervisory system comprises eyepiece (6) and links to each other with computing machine utilizes the illumination path (3-5) of inverted microscope main body, catches the process of cell by eyepiece or the monitoring in real time of CCD camera.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200420085210 CN2758757Y (en) | 2004-10-27 | 2004-10-27 | FS laser clamping device for trapping biological cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200420085210 CN2758757Y (en) | 2004-10-27 | 2004-10-27 | FS laser clamping device for trapping biological cells |
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|---|---|
| CN2758757Y true CN2758757Y (en) | 2006-02-15 |
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|---|---|---|---|
| CN 200420085210 Expired - Fee Related CN2758757Y (en) | 2004-10-27 | 2004-10-27 | FS laser clamping device for trapping biological cells |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100394140C (en) * | 2006-08-09 | 2008-06-11 | 华中科技大学 | Subdivision device of position detection signal |
| CN100453644C (en) * | 2006-12-07 | 2009-01-21 | 天津大学 | Method for femtosecond laser fusion of phaffiafhodozyma cell |
| CN101377965B (en) * | 2007-08-31 | 2011-07-20 | 明基材料股份有限公司 | Disk sheet structure and manufacturing method thereof, and light forceps device using the disk sheet structure |
| CN102023379B (en) * | 2009-09-17 | 2012-07-25 | 中国科学院物理研究所 | Three-dimensional optical tweezers system |
| CN102745643A (en) * | 2011-04-19 | 2012-10-24 | 金石琦 | Laser tweezers device |
| CN102860845A (en) * | 2012-08-30 | 2013-01-09 | 中国科学技术大学 | Method and corresponding device for capturing and controlling in-vivo cells of living body animal |
| CN103344903A (en) * | 2013-06-15 | 2013-10-09 | 浙江大学 | High-temporal-spatial-resolution nerve chip measuring device |
| CN108703138A (en) * | 2018-07-08 | 2018-10-26 | 苏州美丽澄电子技术有限公司 | A kind of method and device freezed in optical tweezer cell and particle to liquid nitrogen |
| CN108873298A (en) * | 2018-07-08 | 2018-11-23 | 苏州美丽澄电子技术有限公司 | A kind of method and device of the anti-fake microcosmic verifying font pattern of optical tweezer particle |
| CN108918520A (en) * | 2018-07-09 | 2018-11-30 | 长沙健金电子技术有限公司 | A kind of devices and methods therefor of fish egg cell screening |
| CN112461830A (en) * | 2020-11-05 | 2021-03-09 | 山东建筑大学 | Combined transparent medium microsphere small-sized optical tweezers device and application |
| CN113227391A (en) * | 2018-12-20 | 2021-08-06 | 慕尼黑应用技术大学 | Laser-induced cell transfer and sorting |
| CN114433262A (en) * | 2022-01-26 | 2022-05-06 | 合肥工业大学 | Multi-particle rapid capturing system and operation method thereof |
-
2004
- 2004-10-27 CN CN 200420085210 patent/CN2758757Y/en not_active Expired - Fee Related
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100394140C (en) * | 2006-08-09 | 2008-06-11 | 华中科技大学 | Subdivision device of position detection signal |
| CN100453644C (en) * | 2006-12-07 | 2009-01-21 | 天津大学 | Method for femtosecond laser fusion of phaffiafhodozyma cell |
| CN101377965B (en) * | 2007-08-31 | 2011-07-20 | 明基材料股份有限公司 | Disk sheet structure and manufacturing method thereof, and light forceps device using the disk sheet structure |
| CN102023379B (en) * | 2009-09-17 | 2012-07-25 | 中国科学院物理研究所 | Three-dimensional optical tweezers system |
| CN102745643A (en) * | 2011-04-19 | 2012-10-24 | 金石琦 | Laser tweezers device |
| CN102860845A (en) * | 2012-08-30 | 2013-01-09 | 中国科学技术大学 | Method and corresponding device for capturing and controlling in-vivo cells of living body animal |
| CN103344903A (en) * | 2013-06-15 | 2013-10-09 | 浙江大学 | High-temporal-spatial-resolution nerve chip measuring device |
| CN103344903B (en) * | 2013-06-15 | 2015-10-28 | 浙江大学 | A kind of neuro chip measurement mechanism of high-spatial and temporal resolution |
| CN108703138A (en) * | 2018-07-08 | 2018-10-26 | 苏州美丽澄电子技术有限公司 | A kind of method and device freezed in optical tweezer cell and particle to liquid nitrogen |
| CN108873298A (en) * | 2018-07-08 | 2018-11-23 | 苏州美丽澄电子技术有限公司 | A kind of method and device of the anti-fake microcosmic verifying font pattern of optical tweezer particle |
| CN108873298B (en) * | 2018-07-08 | 2020-11-27 | 福州宇卓科技有限公司 | Method and device for verifying font and pattern in anti-counterfeiting microscopic mode through optical tweezers particles |
| CN108918520A (en) * | 2018-07-09 | 2018-11-30 | 长沙健金电子技术有限公司 | A kind of devices and methods therefor of fish egg cell screening |
| CN113227391A (en) * | 2018-12-20 | 2021-08-06 | 慕尼黑应用技术大学 | Laser-induced cell transfer and sorting |
| CN112461830A (en) * | 2020-11-05 | 2021-03-09 | 山东建筑大学 | Combined transparent medium microsphere small-sized optical tweezers device and application |
| CN114433262A (en) * | 2022-01-26 | 2022-05-06 | 合肥工业大学 | Multi-particle rapid capturing system and operation method thereof |
| CN114433262B (en) * | 2022-01-26 | 2023-08-22 | 合肥工业大学 | Multi-particle rapid capturing system and operation method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |