CN202522760U - Optical tweezers device of vortex femtosecond laser - Google Patents
Optical tweezers device of vortex femtosecond laser Download PDFInfo
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- CN202522760U CN202522760U CN2012200776150U CN201220077615U CN202522760U CN 202522760 U CN202522760 U CN 202522760U CN 2012200776150 U CN2012200776150 U CN 2012200776150U CN 201220077615 U CN201220077615 U CN 201220077615U CN 202522760 U CN202522760 U CN 202522760U
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Abstract
An optical tweezers device of a vortex femtosecond laser belongs to the optical tweezers technology. The device is characterized in that: a femtosecond laser micro-operation system is taken as a core; seed light output by a femtosecond pulse laser is adjusted by devices of a diaphragm, an attenuation sheet, a total reflection plane mirror and the like and then is transmitted to a vortex raster; through diffraction of the vortex raster, the vortex femtosecond laser is obtained; through a mask slice, first grade of diffraction light is selected so that an optical axis is completely superposed with the optical axis of an imaging optical path of a microscope; the vortex femtosecond laser is coupled into the microscope. By using the device, stable capturing and rotation control of a vortex femtosecond laser pulse with a high repetition rate to particles of a cell, copper oxide, ferric oxide and the like are realized. The device possesses the following advantages that stability is good; the structure is simple and so on. High-precision, non-contact and nondestructive control can be well realized. A possibility is provided for integrating operations of micro-nano devices, such as a micro mechanical motor and the like. The device can be widely used in the micro-control field.
Description
Technical field
The utility model belongs to light tweezer control technology, relates generally to a kind of device that uses the vortex femtosecond laser as light tweezer light source.
Background technology
After people such as Ashkin invent the light tweezer; The light tweezer is owing to have the characteristic of noncontact, not damaged manipulation micro/nano-scale particle; Therefore be widely used in life science, medical science, physics, material and nano science, be considered to optimal unimolecule, unicellular, particulate, micro-nano device operative technique.At present, optical tweezer technology to control object extensive, from transparent dielectric bead, cell, all can realize directly controlling to opaque material such as metal particle, like patent ZL200610078632.5.Compare with Long Pulse LASER with continuous laser, femto-second laser pulse has extremely short pulse width, high peak power, high time and spatial resolution, and control action energy accurately.Calendar year 2001, University Of Tianjin proposed the notion of femtosecond laser light tweezer, compared with continuous light light tweezer, and the optical gradient forces that acts on the particle in the femtosecond light tweezer is a pulsed.In pulse width, the optical force that laser pulse produces is relevant with light intensity, and the optical force that produces constantly in laser pulse peaks reaches extreme value, and in the recurrent interval, particulate does not receive the effect of optical force.Lateral light educational level that femto-second laser pulse produced and axial light educational level can be offset because the influence of the particulate off-centring that Brownian movement causes can realize the stable constraint to particulate.Be that light source can be caught realizations such as erythrocyte, leucocyte, virus, polystyrene microsphere are stable with the high-repetition-rate femtosecond laser at present, like patent ZL200420085210.7.Many in recent years technical staff authors are constantly exploring the various LASER Light Sources of use, are designing different light paths to realize controlling multiple particulate; But most technology all are confined to capture particles and the whole orientation of particulate are moved; Can not realize self rotating of particulate, limit range of application.
Summary of the invention
The purpose of the utility model is to overcome the deficiency of above-mentioned technology; A kind of light forceps device that utilizes the vortex femtosecond laser as light source is proposed; Reach high precision, noncontact, not damageds such as realizing pair cell, particulate and control, and can realize the purpose that rotation is controlled to particulate.
The utility model has adopted following technical scheme to realize: a kind of vortex femtosecond laser light forceps device; On femtosecond pulse laser seed light light path, dispose light hurdle, attenuator and first plane mirror successively; Second plane mirror is configured on the first plane mirror reflected light path; On the second plane mirror reflected light path, dispose shutter and the 3rd plane mirror successively; On Siping City's face mirror reflects light path, dispose microscope, microscope stage and illuminating lamp successively; Lead is connected computing machine respectively with shutter, microscope stage, CCD camera, on the 3rd plane mirror reflected light path, dispose vortex grating and mask plate successively, and a through hole is set on mask plate; The vortex femtosecond laser that Siping City's face mirror arrangement obtains after through the vortex optical grating diffraction is on the one-level vortex femtosecond diffraction light light path that mask plate is selected, and the optical axis of this one-level vortex femtosecond diffraction light light path overlaps with the optical axis of microscope imaging light path fully.The pulse repetition rate of femtosecond pulse laser output is greater than 70 megahertzes, and pulse width is 120 femtoseconds.Microscope stage is three-dimensional micro-displacement platform, and its control accuracy is 0.1 micron.
The major advantage of the utility model is: can realize high repetition frequency vortex femtosecond laser manipulation cell and particulate; For the bigger cell of activity; Compare with Gaussian femtosecond pulse capture technique; It still is annular that the annular hollow characteristics of vortex femtosecond pulse light beam make the hot spot after focusing on distribute; Be easier to realize that high precision, noncontact, not damaged control, thereby be particularly suitable for life science research.With common Gaussian laser optical tweezer compared with techniques, vortex femtosecond light beam has carried orbital angular momentum can be stablized and catch and rotate absorbent particles such as controlling cupric oxide, di-iron trioxide, is the integrated possibility that provides of micro-nano devices such as micromechanics motor operation.
Description of drawings
Fig. 1 is a kind of structural representation of vortex femtosecond laser light forceps device;
Fig. 2 is Fig. 1 mesoscale eddies optical grating construction figure;
Piece number explanation among the figure:
1, femtosecond pulse laser, 2, Guang Lan, 3, attenuator, 4, first plane mirror, 5, second plane mirror, 6, shutter, 7, the 3rd plane mirror, 8, vortex grating, 9, mask plate, 10, Siping City's face catoptron, 11, microscope, 12, microscope stage, 13, illuminating lamp, 14, CCD camera, 15, computing machine.
Embodiment
Below in conjunction with accompanying drawing the utility model optimum implementation is described in detail.A kind of vortex femtosecond laser light forceps device; On femtosecond pulse laser 1 seed light light path, dispose light hurdle 2, attenuator 3 and first plane mirror 4 successively; Second plane mirror 5 is configured on first plane mirror, 4 reflected light paths; On second plane mirror, 5 reflected light paths, dispose shutter 6 and the 3rd plane mirror 7 successively; On Siping City's face catoptron 10 reflected light paths, dispose microscope 11, microscope stage 12 and illuminating lamp 13 successively; Lead is connected computing machine 15 respectively with shutter 6, microscope stage 12, CCD camera 14, on the 3rd plane mirror 7 reflected light paths, dispose vortex grating 8 and mask plate 9 successively, and a through hole is set on mask plate 9; Siping City's face catoptron 10 is configured in through the vortex femtosecond laser that obtains behind vortex grating 8 diffraction on the one-level vortex femtosecond diffraction light light path that mask plate 9 is selected, and the optical axis of this one-level vortex femtosecond diffraction light light path overlaps with the optical axis of microscope 11 imaging optical paths fully.The pulse repetition rate of femtosecond pulse laser 1 output is greater than 70 megahertzes, and pulse width is 120 femtoseconds.Microscope stage 12 is three-dimensional micro-displacement platform, and its control accuracy is 0.1 micron.
Operation process is, the vortex femtosecond laser light beam that the seed light source of femtosecond pulse laser 1 goes out through vortex grating 8 diffraction is as light tweezer light source.From the light pulse of femtosecond pulse laser 1 output is the line polarisation, and its repetition frequency is 76MHz, and pulse width is 120 femtoseconds.Regulate one-level vortex femtosecond diffraction light beam direction, its optical axis is overlapped with the optical axis of microscope 11 imaging optical paths fully, femtosecond vortex laser coupled is got in the microscope 11.
In microscope 11, vortex femtosecond pulse and the reverse propagation of microscope imaging light path tightly focus on through the microscope 11 of high power, form vortex optics potential well.Utilize the microscope stage 12 of three-dimensional micro-displacement platform to control the target particulate in the sample cell on the microscope stages 12, in the optics potential well with its shift-in femtosecond vortex laser, realize the stable of target particulate caught and slewing maneuver.By means of the illuminating lamp 13 of microscope 11 main bodys, can monitor the process that vortex femtosecond laser light tweezer is controlled particulate, show and the recording operation process through CCD camera 14 and computing machine 15.
According to said process, after in the vortex femtosecond pulse light beam coupling entering microscope 11, the human body haemocyte of collecting is merged in the sodium chloride solution, place on the microscope stage 12.Observe red blood cell of selection for 11 times as target at microscope, the target of choosing is applied the vortex femtosecond pulse, realize stable catching.By the front and back of computing machine 15 control microscope stages 12 and move left and right can the relative surrounding environment of relative translation target cell the position; Realized freely controlling in two bit planes, carried out Real Time Observation by microscope 11 eyepieces or through 14 pairs of whole processes of controlling of CCD camera.
The black oxidation copper powders may is placed the sample cell that castor oil is housed, place on the microscope stage 12, observe the cupric oxide particulate 11 times, select a more regular cupric oxide particulate of shape as target at microscope.The cupric oxide particulate of choosing is applied femtosecond vortex laser pulse realize stable catching.The cupric oxide particulate belongs to absorptive particles, and the orbital angular momentum of femtosecond vortex laser beam can shift to the cupric oxide particulate, and particulate can overcome the extraneous moment of resistance directed Periodic Rotating takes place.
Claims (3)
1. vortex femtosecond laser light forceps device; On femtosecond pulse laser (1) seed light light path, dispose light hurdle (2), attenuator (3) and first plane mirror (4) successively; Second plane mirror (5) is configured on first plane mirror (4) reflected light path; On second plane mirror (5) reflected light path, dispose shutter (6) and the 3rd plane mirror (7) successively; On Siping City's face catoptron (10) reflected light path, dispose microscope (11), microscope stage (12) and illuminating lamp (13) successively; Lead is connected computing machine (15) respectively with shutter (6), microscope stage (12), CCD camera (14); It is characterized in that on the 3rd plane mirror (7) reflected light path, disposing successively vortex grating (8) and mask plate (9); A through hole is set on mask plate (9), and Siping City's face catoptron (10) is configured in through the vortex femtosecond laser that obtains behind vortex grating (8) diffraction on the one-level vortex femtosecond diffraction light light path that mask plate (9) is selected, and the optical axis of this one-level vortex femtosecond diffraction light light path overlaps with the optical axis of microscope (11) imaging optical path fully.
2. vortex femtosecond laser light forceps device according to claim 1, the pulse repetition rate that it is characterized in that femtosecond pulse laser (1) output is greater than 70 megahertzes, and pulse width is 120 femtoseconds.
3. vortex femtosecond laser light forceps device according to claim 1 is characterized in that microscope stage (12) is three-dimensional micro-displacement platform, and its control accuracy is 0.1 micron.
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CN2012200776150U CN202522760U (en) | 2012-02-25 | 2012-02-25 | Optical tweezers device of vortex femtosecond laser |
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CN2012200776150U CN202522760U (en) | 2012-02-25 | 2012-02-25 | Optical tweezers device of vortex femtosecond laser |
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Cited By (6)
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CN103913832A (en) * | 2014-04-21 | 2014-07-09 | 黑龙江大学 | Femtosecond laser optical tweezer control device based on cylindrical vector light beams |
CN103913831A (en) * | 2014-04-21 | 2014-07-09 | 黑龙江大学 | Femtosecond laser optical tweezers control device based on S wave plate |
CN103926686A (en) * | 2014-04-21 | 2014-07-16 | 黑龙江大学 | Femtosecond laser mode adjustable optical tweezers control device based on column vector light beam |
CN104793329A (en) * | 2015-04-15 | 2015-07-22 | 黑龙江大学 | Device and method for rotatably controlling optical tweezers by femtosecond laser |
CN105683804A (en) * | 2013-11-01 | 2016-06-15 | 浜松光子学株式会社 | Particle control device |
CN113227391A (en) * | 2018-12-20 | 2021-08-06 | 慕尼黑应用技术大学 | Laser-induced cell transfer and sorting |
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2012
- 2012-02-25 CN CN2012200776150U patent/CN202522760U/en not_active Expired - Fee Related
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105683804A (en) * | 2013-11-01 | 2016-06-15 | 浜松光子学株式会社 | Particle control device |
US9965867B2 (en) | 2013-11-01 | 2018-05-08 | Hamamatsu Photonics K.K. | Particle control device |
CN105683804B (en) * | 2013-11-01 | 2018-09-28 | 浜松光子学株式会社 | Small member control apparatus |
CN103913832A (en) * | 2014-04-21 | 2014-07-09 | 黑龙江大学 | Femtosecond laser optical tweezer control device based on cylindrical vector light beams |
CN103913831A (en) * | 2014-04-21 | 2014-07-09 | 黑龙江大学 | Femtosecond laser optical tweezers control device based on S wave plate |
CN103926686A (en) * | 2014-04-21 | 2014-07-16 | 黑龙江大学 | Femtosecond laser mode adjustable optical tweezers control device based on column vector light beam |
CN104793329A (en) * | 2015-04-15 | 2015-07-22 | 黑龙江大学 | Device and method for rotatably controlling optical tweezers by femtosecond laser |
CN113227391A (en) * | 2018-12-20 | 2021-08-06 | 慕尼黑应用技术大学 | Laser-induced cell transfer and sorting |
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C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
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CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20121107 Termination date: 20130225 |