CN103913831A - Femtosecond laser optical tweezers control device based on S wave plate - Google Patents
Femtosecond laser optical tweezers control device based on S wave plate Download PDFInfo
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- CN103913831A CN103913831A CN201410160702.6A CN201410160702A CN103913831A CN 103913831 A CN103913831 A CN 103913831A CN 201410160702 A CN201410160702 A CN 201410160702A CN 103913831 A CN103913831 A CN 103913831A
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Abstract
The invention provides a femtosecond laser optical tweezers control device based on an S wave plate, and relates to an optical tweezers technology for optically controlling particles and cells. The problems that according to a traditional optical tweezers technology, capture capacity of optical tweezers is improved by increasing incident laser power, and irretrievable heat losses can be caused to a controlled object due to the fact that the capture capacity is improved are solved. A femtosecond pulse laser emits linearly-polarized light, the linearly-polarized light is adjusted through a diaphragm, an attenuation plate and a first 800-nm holophote and then output to a quarter-wave plate, the quarter-wave plate converts the linearly-polarized light into circularly-polarized beams, the S wave plate converts the circularly-polarized beams into columnar vector beams, the columnar vector beams are radially-polarized beams or azimuth angle polarized beams, the columnar vector beams are adjusted through a shutter and a second 800-nm holophote and then enter a microscope, the positions of the parts are adjusted, the optical axis of the columnar vector beams completely coincides with the optical axis of the imaging light path of the microscope, and the beams are coupled into the microscope. The femtosecond laser optical tweezers control device is suitable for the field of life science.
Description
Technical field
The present invention relates to the optical tweezer technology of optical control particle and cell.
Background technology
After optical tweezer technology occurs, light tweezer is owing to having the characteristic of noncontact, not damaged manipulation micro/nano-scale particle, therefore be widely used in life science, medical science, physics, material and nano science, be considered to optimal unimolecule, unicellular, particulate, micro-nano device operative technique.
Optical tweezer technology adopts continuous laser and Long Pulse LASER more, compares with Long Pulse LASER with continuous laser, and femto-second laser pulse has extremely short pulse width, high peak power and time and spatial resolution, and control action energy accurately.Can catch the realizations such as erythrocyte, leucocyte, virus, polystyrene microsphere are stable taking high-repetition-rate femtosecond laser as light source at present.At present the manipulation object of optical tweezer technology is extensive, from transparent dielectric bead, cell, all can realize direct manipulation to opaque material as metal particle.Gaussian beam is traditional light tweezer light source, the optimum working zone of the ligh trap forming after Gaussian beam focusing is near beam focus, many scholars are constantly exploring the various LASER Light Source of use in recent years, design different light paths to realize the optical control to multiple particulate and cell, but most technology are all confined to catching and displacement particulate, limit range of application, simultaneously, traditional optical tweezer technology is the power of catching that improves light tweezer by improving incident laser power, can be to being caused by manipulation object the fire damage that cannot retrieve when the power of catching improves.
Summary of the invention
The present invention is the power of catching that improves light tweezer by improving incident laser power in order to solve traditional optical tweezer technology, when improving, the power of catching can, to caused the problem of the fire damage that cannot retrieve by manipulation object, a kind of femtosecond laser light tweezer actuation means based on S wave plate be proposed.
Femtosecond laser light tweezer actuation means based on S wave plate comprises femtosecond pulse laser, diaphragm, attenuator, a 800nm completely reflecting mirror, quarter-wave plate, S wave plate, shutter, the 2nd 800nm completely reflecting mirror, microscope and an objective table,
The linearly polarized light of femtosecond pulse laser transmitting is incident to attenuator through diaphragm, attenuator carries out, after light intensity attenuation, linearly polarized light is incident to a 800nm completely reflecting mirror to linearly polarized light, the one 800nm completely reflecting mirror by linearly polarized light total reflection to quarter-wave plate, quarter-wave plate is converted to linearly polarized light circularly polarized light and exports S wave plate to, circularly polarized light is converted to radial polarisation light or position angle polarized light by S wave plate, radial polarisation light or position angle polarized light are incident to the 2nd 800nm completely reflecting mirror through shutter, the 2nd 800nm completely reflecting mirror by radial polarisation light or position angle polarized light total reflection to microscopical object lens and be incident on objective table.
Femtosecond laser light tweezer actuation means based on S wave plate also comprises a ccd detector, and described ccd detector is for being converted to the optical image of the light beam between the 2nd 800nm completely reflecting mirror and microscope digital signal and showing.
Femtosecond laser light tweezer actuation means based on S wave plate also comprises a host computer, and described host computer comprises:
Be used for the detectable signal receiver module of the light path detectable signal that receives ccd detector transmission;
For light path detectable signal being converted to the signal conversion module of data message and oscillogram;
For the display module that data message and oscillogram are shown.
Host computer also comprises the objective table control module of carrying out three-dimensional movement for controlling objective table.
Beneficial effect: the linearly polarized light that device of the present invention is launched femtosecond pulse laser by quarter-wave plate and S wave plate is converted to radial polarized light beam and position angle light beam as catching light, column vector beam has unique polarization mode and light distribution, catch light and there is the axial trapping ability of catching light beam higher than Gauss, with respect to using Gaussian beam light tweezer, the use of column vector beam can reach the axial trapping power same with Gaussian beam under less incident laser power regulates, select column vector beam as catching light, the incident laser power that is less than Gaussian beam can reach the axial trapping power same with Gaussian beam, can better avoid the fire damage to being caused by manipulation object.The inhomogeneous orbital angular momentum of conventionally carrying of column vector beam optical field distribution, compared with common Gaussian laser optical tweezer technology, the light beam that carries orbital angular momentum can be stablized and catches and rotate the absorbent particles such as manipulation cupric oxide, di-iron trioxide, is the integrated possibility that provides of the micro-nano device operations such as micromechanics motor.The focusing annular hollow feature of position angle light beam is easy to realize high precision, noncontact, not damaged manipulation, thereby is particularly suitable for life science research.
The present invention uses the light source of femtosecond laser as light forceps device, because femtosecond laser has high time and Spatial resolution, for the power of catching that improves light tweezer provides guarantee.Femtosecond laser technology binding time resolved spectroscopy technology, also can carry out researchs such as the ultrafast bioprocess research of biosome, two-photon fluorescence dynamics.The high time of femtosecond laser and Spatial resolution can also be realized cell without the operation of wound partial modification.
Brief description of the drawings
Fig. 1 is a kind of structural representation of the femtosecond laser light tweezer actuation means based on S wave plate.
Embodiment
Embodiment one, in conjunction with Fig. 1, this embodiment is described, a kind of femtosecond laser light tweezer actuation means based on S wave plate described in this embodiment comprises femtosecond pulse laser 1, diaphragm 2, attenuator 3, a 800nm completely reflecting mirror 4, quarter-wave plate 5, S wave plate 6, shutter 7, the 2nd 800nm completely reflecting mirror 9, microscope 10 and objective table 11
The linearly polarized light that femtosecond pulse laser 1 is launched is incident to attenuator 3 through diaphragm 2, attenuator 3 carries out, after light intensity attenuation, linearly polarized light is incident to a 800nm completely reflecting mirror 4 to linearly polarized light, the one 800nm completely reflecting mirror 4 by linearly polarized light total reflection to quarter-wave plate 5, quarter-wave plate 5 is converted to linearly polarized light circularly polarized light and exports S wave plate 6 to, circularly polarized light is converted to radial polarisation light or position angle polarized light by S wave plate 6, radial polarisation light or position angle polarized light are incident to the 2nd 800nm completely reflecting mirror 9 through shutter 7, the 2nd 800nm completely reflecting mirror 9 by radial polarisation light or position angle polarized light total reflection to the object lens of microscope 10 and be incident on objective table 11.
In present embodiment, adopt femtosecond pulse laser 1 to launch linearly polarized light, linearly polarized light is through diaphragm 2, attenuator 3 and a 800nm completely reflecting mirror 4 export quarter-wave plate 5 to after adjusting, linearly polarized light is converted to circularly polarized light beam by quarter-wave plate 5, circularly polarized light beam is converted to column vector beam by S wave plate 6, this column vector beam is radial polarized light beam or position angle light beam, column vector beam enters in microscope 10 after shutter 7 and the 2nd 800nm completely reflecting mirror 9 adjustment, adjust the position of each parts, column vector beam optical axis is overlapped completely with the optical axis of microscope 10 imaging optical paths, light beam coupling is entered in microscope 10.
In microscope 10, the reverse propagation of imaging optical path of light beam and microscope 10, object lens through the microscope 10 of high power focus on, be converged to the hot spot that radius is less than 1 micron, form optical trap, target particulate on objective table 11 is moved in optical trap, realize the stable of target particulate caught and slewing maneuver.
Quarter-wave plate 5 described in present embodiment is for being converted to linearly polarized light left circularly polarized light or right-circularly polarized light.
S wave plate 6 described in present embodiment is novel superstructure wave plates of being developed by Lithuania, is converted to radially polarization light or position angle polarized light for left circularly polarized light or right-circularly polarized light that quarter-wave plate 5 is exported.
In present embodiment, use the light source of femtosecond laser as light forceps device, because femtosecond laser has high time and Spatial resolution, for the power of catching that improves light tweezer provides guarantee.Femtosecond laser technology binding time resolved spectroscopy technology, also can carry out researchs such as the ultrafast bioprocess research of biosome, two-photon fluorescence dynamics.The high time of femtosecond laser and Spatial resolution can also be realized cell without the operation of wound partial modification.
The difference of a kind of femtosecond laser light tweezer actuation means based on S wave plate described in embodiment two, this embodiment and embodiment one is, described femtosecond pulse laser 1 is titanium-doped sapphire femto-second laser, output pulse repetition rate is 76 megahertzes, and pulse width is 120 femtoseconds.
The difference of a kind of femtosecond laser light tweezer actuation means based on S wave plate described in embodiment three, this embodiment and embodiment one is, described objective table 11 is three-dimensional micro-displacement platform.
The control accuracy of the linear driving source of three-dimensional of the objective table 13 described in present embodiment is 50nm.
Embodiment four, in conjunction with Fig. 1, this embodiment is described, this embodiment is with the difference of a kind of femtosecond laser light tweezer actuation means based on S wave plate described in embodiment one, it also comprises ccd detector 8, and described ccd detector 8 is for being converted to the optical image of the light beam between the 2nd 800nm completely reflecting mirror 9 and microscope 10 digital signal and showing.
In present embodiment, increase ccd detector 8, can carry out Real-Time Monitoring to the light beam of assembling, to know the convergence situation of light beam.
The difference of a kind of femtosecond laser light tweezer actuation means based on S wave plate described in embodiment five, this embodiment and embodiment four is, it also comprises host computer 14, and described host computer 14 comprises:
For receiving the detectable signal receiver module of the light path detectable signal that ccd detector 8 sends;
For light path detectable signal being converted to the signal conversion module of data message and oscillogram;
For the display module that data message and oscillogram are shown.
In present embodiment, the signal that ccd detector 8 can be surveyed by host computer 14 is also complete showing in real time, makes technician can clearly understand light path situation.
The difference of a kind of femtosecond laser light tweezer actuation means based on S wave plate described in embodiment six, this embodiment and embodiment five is, described host computer 14 also comprises for controlling objective table 11 and carries out three-dimensional mobile objective table control module.
The difference of a kind of femtosecond laser light tweezer actuation means based on S wave plate described in embodiment seven, this embodiment and embodiment one is, it also comprises lighting circuit, described lighting circuit comprises power supply 16, switch 17 and illuminating lamp 15, power supply 16, switch 17 and illuminating lamp 15 connect and compose loop successively, illuminating lamp 15 be positioned at objective table 11 under.
In present embodiment, increased lighting circuit, technician, in the time the target particulate on objective table 11 being observed by eyepiece, makes the light on objective table 11 brighter, makes the result of observation more reliable.
Claims (7)
1. the femtosecond laser light tweezer actuation means based on S wave plate, it is characterized in that, it comprises femtosecond pulse laser (1), diaphragm (2), attenuator (3), a 800nm completely reflecting mirror (4), quarter-wave plate (5), S wave plate (6), shutter (7), the 2nd 800nm completely reflecting mirror (9), microscope (10) and objective table (11)
The linearly polarized light of femtosecond pulse laser (1) transmitting is incident to attenuator (3) through diaphragm (2), attenuator (3) carries out, after light intensity attenuation, linearly polarized light is incident to a 800nm completely reflecting mirror (4) to linearly polarized light, the one 800nm completely reflecting mirror (4) by linearly polarized light total reflection to quarter-wave plate (5), quarter-wave plate (5) is converted to circularly polarized light by linearly polarized light and exports S wave plate (6) to, circularly polarized light is converted to radial polarisation light or position angle polarized light by S wave plate (6), radial polarisation light or position angle polarized light are incident to the 2nd 800nm completely reflecting mirror (9) through shutter (7), the 2nd 800nm completely reflecting mirror (9) by radial polarisation light or position angle polarized light total reflection to the object lens of microscope (10) and be incident on objective table (11).
2. a kind of femtosecond laser light tweezer actuation means based on S wave plate according to claim 1, it is characterized in that, described femtosecond pulse laser (1) is titanium-doped sapphire femto-second laser, and output pulse repetition rate is 76 megahertzes, and pulse width is 120 femtoseconds.
3. a kind of femtosecond laser light tweezer actuation means based on S wave plate according to claim 1, is characterized in that, described objective table (11) is three-dimensional micro-displacement platform.
4. a kind of femtosecond laser light tweezer actuation means based on S wave plate according to claim 1, it is characterized in that, it also comprises ccd detector (8), and described ccd detector (8) is for being converted to the optical image of the light beam between the 2nd 800nm completely reflecting mirror (9) and microscope (10) digital signal and showing.
5. a kind of femtosecond laser light tweezer actuation means based on S wave plate according to claim 4, is characterized in that, it also comprises host computer (14), and described host computer (14) comprising:
Be used for the detectable signal receiver module of the light path detectable signal that receives ccd detector (8) transmission;
For light path detectable signal being converted to the signal conversion module of data message and oscillogram;
For the display module that data message and oscillogram are shown.
6. a kind of femtosecond laser light tweezer actuation means based on S wave plate according to claim 5, is characterized in that, described host computer (14) also comprises the objective table control module of carrying out three-dimensional movement for controlling objective table (11).
7. a kind of femtosecond laser light tweezer actuation means based on S wave plate according to claim 1, it is characterized in that, it also comprises lighting circuit, described lighting circuit comprises power supply (16), switch (17) and illuminating lamp (15), power supply (16), switch (17) and illuminating lamp (15) connect and compose loop successively, illuminating lamp (15) be positioned at objective table (11) under.
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| CN201410160702.6A CN103913831A (en) | 2014-04-21 | 2014-04-21 | Femtosecond laser optical tweezers control device based on S wave plate |
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| CN201410160702.6A CN103913831A (en) | 2014-04-21 | 2014-04-21 | Femtosecond laser optical tweezers control device based on S wave plate |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105223000A (en) * | 2015-11-17 | 2016-01-06 | 北京工业大学 | Crystal is to the measuring method of axially symmetry polarization photo-thermal focal length and device |
| CN105511069A (en) * | 2016-02-16 | 2016-04-20 | 东南大学 | Device and method for controlling motion mode of resonant metal nanoparticles |
| CN105742950A (en) * | 2015-12-17 | 2016-07-06 | 武汉光谷科威晶激光技术有限公司 | Device and method for generating angular polarized light |
| RU167405U1 (en) * | 2016-06-08 | 2017-01-10 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Сибирский государственный университет геосистем и технологий" (СГУГиТ) | Mesoscale optical trap in a standing wave field based on two oncoming beams |
| CN109188672A (en) * | 2018-09-12 | 2019-01-11 | 天津大学 | A kind of the controllable rotating operating device and method of optical optical tweezers system |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202522760U (en) * | 2012-02-25 | 2012-11-07 | 黑龙江大学 | Optical tweezers device of vortex femtosecond laser |
| JP2013235122A (en) * | 2012-05-09 | 2013-11-21 | National Institute Of Advanced Industrial & Technology | Three-dimensional operation device for micro object |
| CN203773151U (en) * | 2014-04-21 | 2014-08-13 | 黑龙江大学 | S wave plate-based femtosecond laser optical tweezers manipulation device |
-
2014
- 2014-04-21 CN CN201410160702.6A patent/CN103913831A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202522760U (en) * | 2012-02-25 | 2012-11-07 | 黑龙江大学 | Optical tweezers device of vortex femtosecond laser |
| JP2013235122A (en) * | 2012-05-09 | 2013-11-21 | National Institute Of Advanced Industrial & Technology | Three-dimensional operation device for micro object |
| CN203773151U (en) * | 2014-04-21 | 2014-08-13 | 黑龙江大学 | S wave plate-based femtosecond laser optical tweezers manipulation device |
Non-Patent Citations (3)
| Title |
|---|
| K.J.MOH ET AL: "Generating radial or azimuthal polarization by axial sampling of circularly polarized vortex beams", 《APPLIED OPTICS》 * |
| 冉玲苓: "飞秒激光制备微结构及在粒子操控中的应用研究", 《中国博士学位论文全文数据库信息科技辑》 * |
| 李雪璁: "基于高斯和柱矢量光束光镊的微纳粒子光学捕获及操控", 《中国博士学位论文全文数据库工程科技I辑》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105223000A (en) * | 2015-11-17 | 2016-01-06 | 北京工业大学 | Crystal is to the measuring method of axially symmetry polarization photo-thermal focal length and device |
| CN105223000B (en) * | 2015-11-17 | 2017-10-20 | 北京工业大学 | Measuring method and device of the crystal to axially symmetry polarization photo-thermal focal length |
| CN105742950A (en) * | 2015-12-17 | 2016-07-06 | 武汉光谷科威晶激光技术有限公司 | Device and method for generating angular polarized light |
| CN105511069A (en) * | 2016-02-16 | 2016-04-20 | 东南大学 | Device and method for controlling motion mode of resonant metal nanoparticles |
| CN105511069B (en) * | 2016-02-16 | 2018-03-13 | 东南大学 | A kind of method for manipulating resonance metal nanoparticle motion mode |
| RU167405U1 (en) * | 2016-06-08 | 2017-01-10 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Сибирский государственный университет геосистем и технологий" (СГУГиТ) | Mesoscale optical trap in a standing wave field based on two oncoming beams |
| CN109188672A (en) * | 2018-09-12 | 2019-01-11 | 天津大学 | A kind of the controllable rotating operating device and method of optical optical tweezers system |
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Application publication date: 20140709 |