CN203773151U - S wave plate-based femtosecond laser optical tweezers manipulation device - Google Patents
S wave plate-based femtosecond laser optical tweezers manipulation device Download PDFInfo
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- CN203773151U CN203773151U CN201420194786.0U CN201420194786U CN203773151U CN 203773151 U CN203773151 U CN 203773151U CN 201420194786 U CN201420194786 U CN 201420194786U CN 203773151 U CN203773151 U CN 203773151U
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Abstract
The utility model, which relates to the optical tweezers technology of the optical manipulation particle and cell, provides an S wave plate-based femtosecond laser optical tweezers manipulation device. According to the traditional optical tweezers technology, the trapping capability of the optical tweezers is improved by enhancing the incident laser power and thus although the trapping capability is improved, the irretrievable thermal damage will be caused to the manipulated object. However, with the device, the above-mentioned problem can be solved. A femtosecond pulse laser is used for emitting linearly polarized light; the linearly polarized light is adjusted by a diaphragm, an attenuation plate, and a first 800-nm total reflection mirror and then is outputted to a quarter-wave plate; the quarter-wave plate converts the processed linearly polarized light into a circularly polarized light beam; an S wave plate converts the circularly polarized light beam into a cylindrical vector light beam that is a radially polarized light beam or an azimuthal polarization light beam; the cylindrical vector light beam is adjusted by a shutter and a second 800-nm total reflection mirror and then enters a microscope; positions of all components are adjusted and thus the optical axis of the cylindrical vector light beam is completely superposed with the optical axis of the imaging light path of the microscope; and the light beam is coupled to the microscope. The provided S wave plate-based femtosecond laser optical tweezers manipulation device is suitable for the life sciences field.
Description
Technical field
The utility model relates to the optical tweezer technology of optical control particle and cell.
Background technology
After optical tweezer technology occurs, light tweezer is owing to having the characteristic of noncontact, not damaged manipulation micro/nano-scale particle, therefore be widely used in life science, medical science, physics, material and nano science, be considered to optimal unimolecule, unicellular, particulate, micro-nano device operative technique.
Optical tweezer technology adopts continuous laser and Long Pulse LASER more, compares with Long Pulse LASER with continuous laser, and femto-second laser pulse has extremely short pulse width, high peak power and time and spatial resolution, and control action energy accurately.Can catch the realizations such as erythrocyte, leucocyte, virus, polystyrene microsphere are stable taking high-repetition-rate femtosecond laser as light source at present.At present the manipulation object of optical tweezer technology is extensive, from transparent dielectric bead, cell, all can realize direct manipulation to opaque material as metal particle.Gaussian beam is traditional light tweezer light source, the optimum working zone of the ligh trap forming after Gaussian beam focusing is near beam focus, many scholars are constantly exploring the various LASER Light Source of use in recent years, design different light paths to realize the optical control to multiple particulate and cell, but most technology are all confined to catching and displacement particulate, limit range of application, simultaneously, traditional optical tweezer technology is the power of catching that improves light tweezer by improving incident laser power, can be to being caused by manipulation object the fire damage that cannot retrieve when the power of catching improves.
Utility model content
The utility model is the power of catching that improves light tweezer by improving incident laser power in order to solve traditional optical tweezer technology, when improving, the power of catching can, to caused the problem of the fire damage that cannot retrieve by manipulation object, a kind of femtosecond laser light tweezer actuation means based on S wave plate be proposed.
Femtosecond laser light tweezer actuation means based on S wave plate comprises femtosecond pulse laser, diaphragm, attenuator, a 800nm completely reflecting mirror, quarter-wave plate, S wave plate, shutter, the 2nd 800nm completely reflecting mirror, microscope and an objective table,
The linearly polarized light of femtosecond pulse laser transmitting is incident to attenuator through diaphragm, attenuator carries out, after light intensity attenuation, linearly polarized light is incident to a 800nm completely reflecting mirror to linearly polarized light, the one 800nm completely reflecting mirror by linearly polarized light total reflection to quarter-wave plate, quarter-wave plate is converted to linearly polarized light circularly polarized light and exports S wave plate to, circularly polarized light is converted to radial polarisation light or position angle polarized light by S wave plate, radial polarisation light or position angle polarized light are incident to the 2nd 800nm completely reflecting mirror through shutter, the 2nd 800nm completely reflecting mirror by radial polarisation light or position angle polarized light total reflection to microscopical object lens and be incident on objective table.
Femtosecond laser light tweezer actuation means based on S wave plate also comprises a ccd detector, and described ccd detector is for being converted to the optical image of the light beam between the 2nd 800nm completely reflecting mirror and microscope digital signal and showing.
Beneficial effect: the linearly polarized light that device described in the utility model is launched femtosecond pulse laser by quarter-wave plate and S wave plate is converted to radial polarized light beam and position angle light beam as catching light, column vector beam has unique polarization mode and light distribution, catch light and there is the axial trapping ability of catching light beam higher than Gauss, with respect to using Gaussian beam light tweezer, the use of column vector beam can reach the axial trapping power same with Gaussian beam under less incident laser power regulates, select column vector beam as catching light, the incident laser power that is less than Gaussian beam can reach the axial trapping power same with Gaussian beam, can better avoid the fire damage to being caused by manipulation object.The inhomogeneous orbital angular momentum of conventionally carrying of column vector beam optical field distribution, compared with common Gaussian laser optical tweezer technology, the light beam that carries orbital angular momentum can be stablized and catches and rotate the absorbent particles such as manipulation cupric oxide, di-iron trioxide, is the integrated possibility that provides of the micro-nano device operations such as micromechanics motor.The focusing annular hollow feature of position angle light beam is easy to realize high precision, noncontact, not damaged manipulation, thereby is particularly suitable for life science research.
The utility model uses the light source of femtosecond laser as light forceps device, because femtosecond laser has high time and Spatial resolution, for the power of catching that improves light tweezer provides guarantee.Femtosecond laser technology binding time resolved spectroscopy technology, also can carry out researchs such as the ultrafast bioprocess research of biosome, two-photon fluorescence dynamics.The high time of femtosecond laser and Spatial resolution can also be realized cell without the operation of wound partial modification.
Brief description of the drawings
Fig. 1 is a kind of structural representation of the femtosecond laser light tweezer actuation means based on S wave plate.
Embodiment
Embodiment one, in conjunction with Fig. 1, this embodiment is described, a kind of femtosecond laser light tweezer actuation means based on S wave plate described in this embodiment comprises femtosecond pulse laser 1, diaphragm 2, attenuator 3, a 800nm completely reflecting mirror 4, quarter-wave plate 5, S wave plate 6, shutter 7, the 2nd 800nm completely reflecting mirror 9, microscope 10 and objective table 11
The linearly polarized light that femtosecond pulse laser 1 is launched is incident to attenuator 3 through diaphragm 2, attenuator 3 carries out, after light intensity attenuation, linearly polarized light is incident to a 800nm completely reflecting mirror 4 to linearly polarized light, the one 800nm completely reflecting mirror 4 by linearly polarized light total reflection to quarter-wave plate 5, quarter-wave plate 5 is converted to linearly polarized light circularly polarized light and exports S wave plate 6 to, circularly polarized light is converted to radial polarisation light or position angle polarized light by S wave plate 6, radial polarisation light or position angle polarized light are incident to the 2nd 800nm completely reflecting mirror 9 through shutter 7, the 2nd 800nm completely reflecting mirror 9 by radial polarisation light or position angle polarized light total reflection to the object lens of microscope 10 and be incident on objective table 11.
In present embodiment, adopt femtosecond pulse laser 1 to launch linearly polarized light, linearly polarized light is through diaphragm 2, attenuator 3 and a 800nm completely reflecting mirror 4 export quarter-wave plate 5 to after adjusting, linearly polarized light is converted to circularly polarized light beam by quarter-wave plate 5, circularly polarized light beam is converted to column vector beam by S wave plate 6, this column vector beam is radial polarized light beam or position angle light beam, column vector beam enters in microscope 10 after shutter 7 and the 2nd 800nm completely reflecting mirror 9 adjustment, adjust the position of each parts, column vector beam optical axis is overlapped completely with the optical axis of microscope 10 imaging optical paths, light beam coupling is entered in microscope 10.
In microscope 10, the reverse propagation of imaging optical path of light beam and microscope 10, object lens through the microscope 10 of high power focus on, be converged to the hot spot that radius is less than 1 micron, form optical trap, target particulate on objective table 11 is moved in optical trap, realize the stable of target particulate caught and slewing maneuver.
Quarter-wave plate 5 described in present embodiment is for being converted to linearly polarized light left circularly polarized light or right-circularly polarized light.
S wave plate 6 described in present embodiment is novel superstructure wave plates of being developed by Lithuania, is converted to radially polarization light or position angle polarized light for left circularly polarized light or right-circularly polarized light that quarter-wave plate 5 is exported.
In present embodiment, use the light source of femtosecond laser as light forceps device, because femtosecond laser has high time and Spatial resolution, for the power of catching that improves light tweezer provides guarantee.Femtosecond laser technology binding time resolved spectroscopy technology, also can carry out researchs such as the ultrafast bioprocess research of biosome, two-photon fluorescence dynamics.The high time of femtosecond laser and Spatial resolution can also be realized cell without the operation of wound partial modification.
The difference of a kind of femtosecond laser light tweezer actuation means based on S wave plate described in embodiment two, this embodiment and embodiment one is, described femtosecond pulse laser 1 is titanium-doped sapphire femto-second laser, output pulse repetition rate is 76 megahertzes, and pulse width is 120 femtoseconds.
The difference of a kind of femtosecond laser light tweezer actuation means based on S wave plate described in embodiment three, this embodiment and embodiment one is, described objective table 11 is three-dimensional micro-displacement platform.
The control accuracy of the linear driving source of three-dimensional of the objective table 13 described in present embodiment is 50nm.
Embodiment four, in conjunction with Fig. 1, this embodiment is described, this embodiment is with the difference of a kind of femtosecond laser light tweezer actuation means based on S wave plate described in embodiment one, it also comprises ccd detector 8, and described ccd detector 8 is for being converted to the optical image of the light beam between the 2nd 800nm completely reflecting mirror 9 and microscope 10 digital signal and showing.
In present embodiment, increase ccd detector 8, can carry out Real-Time Monitoring to the light beam of assembling, to know the convergence situation of light beam.
The difference of a kind of femtosecond laser light tweezer actuation means based on S wave plate described in embodiment five, this embodiment and embodiment one is, it also comprises lighting circuit, described lighting circuit comprises power supply 16, switch 17 and illuminating lamp 15, power supply 16, switch 17 and illuminating lamp 15 connect and compose loop successively, illuminating lamp 15 be positioned at objective table 11 under.
In present embodiment, increased lighting circuit, technician, in the time the target particulate on objective table 11 being observed by eyepiece, makes the light on objective table 11 brighter, makes the result of observation more reliable.
Claims (5)
1. the femtosecond laser light tweezer actuation means based on S wave plate, it is characterized in that, it comprises femtosecond pulse laser (1), diaphragm (2), attenuator (3), a 800nm completely reflecting mirror (4), quarter-wave plate (5), S wave plate (6), shutter (7), the 2nd 800nm completely reflecting mirror (9), microscope (10) and objective table (11)
The linearly polarized light of femtosecond pulse laser (1) transmitting is incident to attenuator (3) through diaphragm (2), attenuator (3) carries out, after light intensity attenuation, linearly polarized light is incident to a 800nm completely reflecting mirror (4) to linearly polarized light, the one 800nm completely reflecting mirror (4) by linearly polarized light total reflection to quarter-wave plate (5), quarter-wave plate (5) is converted to circularly polarized light by linearly polarized light and exports S wave plate (6) to, circularly polarized light is converted to radial polarisation light or position angle polarized light by S wave plate (6), radial polarisation light or position angle polarized light are incident to the 2nd 800nm completely reflecting mirror (9) through shutter (7), the 2nd 800nm completely reflecting mirror (9) by radial polarisation light or position angle polarized light total reflection to the object lens of microscope (10) and be incident on objective table (11).
2. a kind of femtosecond laser light tweezer actuation means based on S wave plate according to claim 1, it is characterized in that, described femtosecond pulse laser (1) is titanium-doped sapphire femto-second laser, and output pulse repetition rate is 76 megahertzes, and pulse width is 120 femtoseconds.
3. a kind of femtosecond laser light tweezer actuation means based on S wave plate according to claim 1, is characterized in that, described objective table (11) is three-dimensional micro-displacement platform.
4. a kind of femtosecond laser light tweezer actuation means based on S wave plate according to claim 1, it is characterized in that, it also comprises ccd detector (8), and described ccd detector (8) is for being converted to the optical image of the light beam between the 2nd 800nm completely reflecting mirror (9) and microscope (10) digital signal and showing.
5. a kind of femtosecond laser light tweezer actuation means based on S wave plate according to claim 1, it is characterized in that, it also comprises lighting circuit, described lighting circuit comprises power supply (16), switch (17) and illuminating lamp (15), power supply (16), switch (17) and illuminating lamp (15) connect and compose loop successively, illuminating lamp (15) be positioned at objective table (11) under.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201420194786.0U CN203773151U (en) | 2014-04-21 | 2014-04-21 | S wave plate-based femtosecond laser optical tweezers manipulation device |
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| CN201420194786.0U CN203773151U (en) | 2014-04-21 | 2014-04-21 | S wave plate-based femtosecond laser optical tweezers manipulation device |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103913831A (en) * | 2014-04-21 | 2014-07-09 | 黑龙江大学 | Femtosecond laser optical tweezers control device based on S wave plate |
| CN105116534A (en) * | 2015-07-21 | 2015-12-02 | 大连理工大学 | Method for capturing and screening particle above topological insulator substrate in tunable manner through linearly-polarized planar optical wave |
| CN105118541A (en) * | 2015-07-21 | 2015-12-02 | 大连理工大学 | Tunable capturing and screening method of linear polarization planar optical waves for particle located above chalcogenide substrate |
| CN106526154A (en) * | 2016-11-30 | 2017-03-22 | 江苏师范大学 | Measurement method of erythrocyte shear modulus, and measurement method of oxygen carrying capacity of blood |
-
2014
- 2014-04-21 CN CN201420194786.0U patent/CN203773151U/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103913831A (en) * | 2014-04-21 | 2014-07-09 | 黑龙江大学 | Femtosecond laser optical tweezers control device based on S wave plate |
| CN105116534A (en) * | 2015-07-21 | 2015-12-02 | 大连理工大学 | Method for capturing and screening particle above topological insulator substrate in tunable manner through linearly-polarized planar optical wave |
| CN105118541A (en) * | 2015-07-21 | 2015-12-02 | 大连理工大学 | Tunable capturing and screening method of linear polarization planar optical waves for particle located above chalcogenide substrate |
| CN105118541B (en) * | 2015-07-21 | 2017-04-12 | 大连理工大学 | Tunable capturing and screening method of linear polarization planar optical waves for particle located above chalcogenide substrate |
| CN106526154A (en) * | 2016-11-30 | 2017-03-22 | 江苏师范大学 | Measurement method of erythrocyte shear modulus, and measurement method of oxygen carrying capacity of blood |
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Granted publication date: 20140813 Termination date: 20190421 |
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| CF01 | Termination of patent right due to non-payment of annual fee |