CN102095690A - Polarization imaging nondestructive detection device - Google Patents
Polarization imaging nondestructive detection device Download PDFInfo
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- CN102095690A CN102095690A CN 201010596940 CN201010596940A CN102095690A CN 102095690 A CN102095690 A CN 102095690A CN 201010596940 CN201010596940 CN 201010596940 CN 201010596940 A CN201010596940 A CN 201010596940A CN 102095690 A CN102095690 A CN 102095690A
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Abstract
The invention relates to a polarization imaging nondestructive detection semi-automatic device. The device is characterized by comprising a laser, a polarization detection system, a light path transmission system, an object stage, a detection system and a calculation processing system; the laser beam emitted by the laser sequentially passes through the polarization detection system, the light path transmission system and the object stage; the laser beam can generate secondary harmonic signal light and fluorescent signals when irradiating the sample on the object stage, then returns to the light path transmission system and the polarization detection system, and arrives at the detection system; the detection system and the light path transmission system are controlled by a computer; and the polarization detection system and the object stage are controlled manually. The device can rapidly and nondestructively detect materials with birefringence effect, such as wafer, biological tissues and plants in real time, and can obtain high-resolution and high-contrast images and the intensity and direction of the images.
Description
Technical field
The present invention relates to a kind of lossless detection device of polarization imaging, can be used for differentiating and have birefringence biological tissue fine structure and other birefringence material, belong to emerging cross discipline---biomedical optics in the present age, relate to fields such as optics, acoustics, electronic information, computer technology and medical science.
Background technology
For the method for biological tissue's hyperfine structure, mainly contain electron microscope at present, X-ray, biochemical analysis, methods such as histopathological analysis, multiphoton fluorescence technology.Electron microscope wherein, the X-ray, biochemical analysis, the shortcoming of method maximums such as histopathological analysis be have serious damaging, and can't be in real time, the detection of live body.Although and can obtain high resolving power in real non-destructive ground by the multiphoton fluorescence technology of multi-photon microscopy combination, the microstructure of high-contrast has very high peak power and lower average power, reduces or eliminates photobleaching and light toxic action.Yet it can't obtain the ratio of epithelial tissue collagen-type and the directivity information of collagen.And for the biological epithelial tissue pathology with birefringence of majority, such as tumour, this needs in time to obtain the directivity information after the collagen pathology of its tumour epithelial tissue, with the structure and the direction of growth that obtains pathological tissues more accurately.Therefore, the method that need to explore a kind of live body, can tell the institutional framework directivity in real time again, thereby comprise the biological tissue of tumour, the material and the various mineral matter of chemical field in the field of biology, realize real-time, the harmless detection by quantitative of ability of its birefringence.
Summary of the invention:
The objective of the invention is for biological tissue with birefringence etc. provide a kind of harmless, in real time, dynamic sniffer, the second harmonic signal that utilizes the character of polarisation to launch in conjunction with light and matter interaction carries out micro-structure imaging and analysis to the material with birefringence.This device can carry out the detection of high precision, high resolving power, high-contrast to the microstructure of biological tissue and crystal etc., and obtains its directivity information.
Technical scheme implementation method of the present invention: the lossless detection device is made of laser instrument, Polarization Detection system, optic path system, objective table, detection system and computing system.Laser instrument emitted laser bundle is successively by the polarizer, optic path system, objective table; Back generation second harmonic signal light and fluorescence signal and reverse optical path transmission system, Polarization Detection system arrive detection system on the sample of laser beam arrival objective table; Wherein detection system, the optic path system can be by computer control, Polarization Detection system and objective table are then by manual control.
Described laser instrument is a tunable femto-second laser.This laser instrument institute emitted light beams wavelength coverage is: 700nm-980nm.
Described Polarization Detection system is made up of with the device that comprises quarter wave plate 14, analyzer and relevant Electromagnetic Control unit the polarizer.Laser beam forms the incident light of particular polarization through the polarizer and after shaping, and is accepted by detection system through beam splitter, quarter wave plate 14, analyzer etc. from the reception light of sample.Through obtaining comprising the information of sample polarization structure behind the conversed analysis.
Described optic path system includes X-Y scanner, amplification imaging device, dichroscope, object lens, optical filter, condenser A and condenser B and forms.Light through the polarizer in the optic path system is received by the X-Y scanner, enters the amplification imaging device subsequently.The amplification imaging device is made up of lens A and convex lens B, can realize the amplification of light beam or dwindles.Laser beam through amplifying, through dichroiscopic reflection, by object lens focusing to the sample of rotatable stage and produce second harmonic signal light and fluorescence signal, second harmonic signal light and fluorescence signal will be oppositely by object lens and directly through dichroscope, incide on the optical filter, because the wavelength selection effect of optical filter, fluorescence signal and second harmonic signal light will be divided into 2 the tunnel, and fluorescent signal light arrives detection system through a condenser A after directly seeing through optical filter; Second harmonic signal light then reflect the back by behind the condenser B by arriving detection system behind the analyzer.
Described detection system is made up of photomultiplier A, photomultiplier B.Photomultiplier A receives fluorescence signal, and photomultiplier B receives the second harmonic signal that comprises polarization information.Detection system and optic path system are all controlled by computing machine, can be shown simultaneously by computing machine to contain polarization information image and conventional non-linear or fluoroscopic image.
Described objective table is rotatable objective table, places sample on it, and sample produces the second harmonic signal light and the fluorescence signal of different polarization dominant direction behind laser beam irradiation.
Described dichroscope can reflect the light greater than the 700nm wavelength, and transmissive is less than the light of 700nm wavelength.
Described optical filter can reflect the light less than 450nm, and transmissive is greater than the light of 450nm.
Apparatus of the present invention can be real-time, and fast, nondestructively detection of biological tissue, plant and crystal etc. have the material of birefringence, and obtain image and the intensity and the direction of high resolving power, high-contrast.Its gordian technique is the application of polarization analysis method, makes when obtaining the second harmonic signal of polarization, can rotate polarizer control incident light polarization direction as required by the polarization information that can obtain to add, and the sample of objective table can also rotate simultaneously.The used ultra-short pulse laser bundle that only passes through the polarization module of imaging of tissue produces.And polarizing method is the fluorescent emission that the angle in the parallel direction of polarization and horizontal direction excites polarization axle to obtain.Sample excitation and emission dipole direction can be determined.Simultaneously also can obtain fluorescence polarization spectrum.
Its advantage of apparatus of the present invention is in conjunction with polarization characteristic, obtain the microstructure of Ultrastructural disdiaclast, obtaining the second harmonic intensity of disdiaclast, the directivity information of its tissue and the parameter of other quadratic nonlinearity receptor simultaneously, is to comprise the biological tissue of tumour and the ability of the various materials of chemical field and its birefringence of mineral matter detection by quantitative thereby possess.
Description of drawings
Fig. 1 is the structure principle chart of apparatus of the present invention.
Fig. 2 is the concrete organigrams of implementing of apparatus of the present invention.
Embodiment
For the present invention there being further understanding, be explained in detail below in conjunction with 2 pairs of embodiments of the invention of accompanying drawing.
Among Fig. 2,1. laser instrument; 2. the polarizer; 3.X-Y scanning system; 4. convex lens A; 5. convex lens B; 6. rotatable stage; 7. object lens; 8. dichroscope; 9. quarter wave plate 14, analyzer and relevant Electromagnetic Control unit; 10. condenser B; 11. optical filter; 12. condenser A; 13. photomultiplier A; 14. computing machine; 15. photomultiplier B.
In enforcement of the present invention, be sample with dermal layer of the skin collagen; Selecting the wavelength of best tunable laser 1 is 850nm, because this wavelength can excite second harmonic signal preferably.Laser becomes a linearly polarized light that can change direction after by the polarizer 2.Because dichroscope 8 can reflect the light greater than 700nm, so the skin samples that light reflection back arrives on the objective table 6 by object lens 7.Laser and birefringent collagen medium interaction produce the former road of second harmonic light and return.Certainly, corium also has other materials, and such as elastic fibers, the fluorescence that produces after itself and light interact also is that former road is returned.Fluorescence and second harmonic light arrive dichroscope 8 backs less than the transmittance of 700nm after filter mating plate 11.The plated film of optical filter 11 is less than the reflection of the light of 450nm, and to the only transmission of 450nm-650nm.So to directly being received after by a condenser A12 by photomultiplier A13 greater than the fluorescence of 450nm, the light that produces for second-harmonic generation is then by needing behind the focus lamp B10 through quarter wave plate 14, analyzer and relevant Electromagnetic Control unit 9, received by photomultiplier B15 behind its polarization state of analyzing and testing, obtain the variation of material birefringent fiber, so on computing machine 14, can obtain the directivity of sample in the demonstration second harmonic intensity.The method can be quick, live body, and detection of biological is organized in the situation of change of collagen direction under pathology or the photo-thermal damage in real time.
Claims (9)
1. the lossless detection device of a polarization imaging, it is characterized in that: device is made of laser instrument, Polarization Detection system, optic path system, objective table, detection system and computing system, laser instrument emitted laser bundle is successively by the polarizer, optic path system, objective table; Back generation second harmonic signal light and fluorescence signal and reverse optical path transmission system, Polarization Detection system arrive detection system on the sample of laser beam arrival objective table; Detection system, optic path system are by computer control, and Polarization Detection system and objective table are then by manual control.
2. the lossless detection device of a kind of polarization imaging according to claim 1 is characterized in that described laser instrument is tunable femto-second laser, and wavelength coverage is: 700nm～980nm.
3. the lossless detection device of a kind of polarization imaging according to claim 1, it is characterized by: described dichroscope can reflect the light greater than the 700nm wavelength, and transmissive is less than the light of 700nm.
4. the lossless detection device of a kind of polarization imaging according to claim 1, it is characterized by: optical filter can reflect the light less than 450nm, and transmission is greater than the light of 450nm.
5. the lossless detection device of a kind of polarization imaging according to claim 1 is characterized by: described optic path system includes X-Y scanner, amplification imaging device, dichroscope, object lens, optical filter, condenser A and condenser B and forms; X-Y scanner in the optic path system receives the light that comes from the polarizer; Laser beam through the amplification imaging device, pass through dichroiscopic reflection again, to the sample of rotatable stage and produce second harmonic signal light and fluorescence signal, second harmonic signal light and fluorescence signal will incide on the optical filter oppositely by object lens and directly through dichroscope by object lens focusing; Fluorescence signal and second harmonic signal light will be divided into 2 the tunnel, and fluorescent signal light arrives detection system through a condenser A after directly seeing through optical filter; Second harmonic signal light then reflect the back by behind the condenser B by arriving detection system behind the analyzer.
6. the lossless detection device of a kind of polarization imaging according to claim 5, it is characterized by: described amplification imaging device is made up of lens A and convex lens B.
7. the lossless detection device of a kind of polarization imaging according to claim 1 is characterized by: described Polarization Detection system is made up of with the device that comprises quarter wave plate 14, analyzer and relevant Electromagnetic Control unit the polarizer; Laser beam forms the incident light of particular polarization through the polarizer and after shaping, accepted by detection system through beam splitter, quarter wave plate 14, analyzer etc. and receive light; Through obtaining comprising the information of sample polarization structure behind the conversed analysis.
8. the lossless detection device of a kind of polarization imaging according to claim 7 is characterized by the described polarizer and the Polarization Detection system unit all can be rotated.
9. the lossless detection device of a kind of polarization imaging according to claim 1, it is characterized by: described objective table is rotatable objective table, to produce the second harmonic signal light and the fluorescence signal of different polarization dominant direction.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103471989A (en) * | 2013-01-08 | 2013-12-25 | 刘学峰 | Optical vector modeling based non-visual imaging method |
| CN104136076A (en) * | 2012-02-28 | 2014-11-05 | 皇家飞利浦有限公司 | Device for energy-based skin treatment |
| CN106526823A (en) * | 2016-11-09 | 2017-03-22 | 南京理工大学 | DNA nanosphere non-fluorescent non-visual microscopic imaging device and method |
| CN107024427A (en) * | 2017-04-17 | 2017-08-08 | 金华职业技术学院 | A kind of device for the tribology for studying monolayer |
| CN110638424A (en) * | 2019-09-19 | 2020-01-03 | 哈尔滨工业大学 | Scanning light sheet harmonic wave microscopic imaging method and device |
| CN110664369A (en) * | 2019-09-19 | 2020-01-10 | 哈尔滨工业大学 | Self-adaptive confocal line scanning harmonic microscopic imaging method and device |
| CN110907471A (en) * | 2018-09-18 | 2020-03-24 | 三星电子株式会社 | Method of detecting defects on a substrate and apparatus for detecting defects on a substrate |
| CN110932789A (en) * | 2019-12-20 | 2020-03-27 | 武汉光迅科技股份有限公司 | Wavelength tunable device packaging structure |
| CN114397283A (en) * | 2022-01-19 | 2022-04-26 | 天津大学 | Detection system and method for in-situ combination of secondary harmonic and fluorescence spectrum |
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| US6208886B1 (en) * | 1997-04-04 | 2001-03-27 | The Research Foundation Of City College Of New York | Non-linear optical tomography of turbid media |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104136076A (en) * | 2012-02-28 | 2014-11-05 | 皇家飞利浦有限公司 | Device for energy-based skin treatment |
| CN104136076B (en) * | 2012-02-28 | 2017-03-15 | 皇家飞利浦有限公司 | Device for the skin treatment based on energy |
| CN103471989A (en) * | 2013-01-08 | 2013-12-25 | 刘学峰 | Optical vector modeling based non-visual imaging method |
| CN103471989B (en) * | 2013-01-08 | 2016-02-03 | 刘学峰 | A kind of non-intuitive formation method based on optics arrow pattern |
| CN106526823B (en) * | 2016-11-09 | 2018-11-13 | 南京理工大学 | A kind of non-fluorescence non-intuitive microscopic imaging device of DNA nanospheres and method |
| CN106526823A (en) * | 2016-11-09 | 2017-03-22 | 南京理工大学 | DNA nanosphere non-fluorescent non-visual microscopic imaging device and method |
| CN107024427A (en) * | 2017-04-17 | 2017-08-08 | 金华职业技术学院 | A kind of device for the tribology for studying monolayer |
| CN110907471A (en) * | 2018-09-18 | 2020-03-24 | 三星电子株式会社 | Method of detecting defects on a substrate and apparatus for detecting defects on a substrate |
| CN110638424A (en) * | 2019-09-19 | 2020-01-03 | 哈尔滨工业大学 | Scanning light sheet harmonic wave microscopic imaging method and device |
| CN110664369A (en) * | 2019-09-19 | 2020-01-10 | 哈尔滨工业大学 | Self-adaptive confocal line scanning harmonic microscopic imaging method and device |
| CN110638424B (en) * | 2019-09-19 | 2022-05-13 | 哈尔滨工业大学 | Scanning light sheet harmonic wave microscopic imaging method and device |
| CN110932789A (en) * | 2019-12-20 | 2020-03-27 | 武汉光迅科技股份有限公司 | Wavelength tunable device packaging structure |
| CN114397283A (en) * | 2022-01-19 | 2022-04-26 | 天津大学 | Detection system and method for in-situ combination of secondary harmonic and fluorescence spectrum |
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Application publication date: 20110615 |