CN110208227A - A kind of list object lens mating plate micro imaging system - Google Patents
A kind of list object lens mating plate micro imaging system Download PDFInfo
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- CN110208227A CN110208227A CN201910395664.5A CN201910395664A CN110208227A CN 110208227 A CN110208227 A CN 110208227A CN 201910395664 A CN201910395664 A CN 201910395664A CN 110208227 A CN110208227 A CN 110208227A
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- 230000013011 mating Effects 0.000 title claims abstract description 97
- 238000003384 imaging method Methods 0.000 title claims abstract description 30
- 230000005284 excitation Effects 0.000 claims abstract description 8
- 230000003213 activating effect Effects 0.000 claims abstract description 6
- 239000003086 colorant Substances 0.000 claims description 2
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 230000003287 optical effect Effects 0.000 abstract description 11
- 238000000399 optical microscopy Methods 0.000 abstract description 2
- 239000000523 sample Substances 0.000 description 34
- 241000252212 Danio rerio Species 0.000 description 5
- 238000000799 fluorescence microscopy Methods 0.000 description 4
- 241000255581 Drosophila <fruit fly, genus> Species 0.000 description 3
- 239000004606 Fillers/Extenders Substances 0.000 description 3
- 210000002257 embryonic structure Anatomy 0.000 description 3
- 210000001161 mammalian embryo Anatomy 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000000386 microscopy Methods 0.000 description 3
- 239000012472 biological sample Substances 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 206010034972 Photosensitivity reaction Diseases 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- 241000255588 Tephritidae Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000000571 coke Substances 0.000 description 1
- 238000007334 copolymerization reaction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 239000010410 layer Substances 0.000 description 1
- 208000007578 phototoxic dermatitis Diseases 0.000 description 1
- 231100000018 phototoxicity Toxicity 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000011241 protective layer Substances 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000004381 surface treatment Methods 0.000 description 1
- 238000003325 tomography Methods 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1434—Optical arrangements
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1434—Optical arrangements
- G01N2015/144—Imaging characterised by its optical setup
- G01N2015/1445—Three-dimensional imaging, imaging in different image planes, e.g. under different angles or at different depths, e.g. by a relative motion of sample and detector, for instance by tomography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
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- Life Sciences & Earth Sciences (AREA)
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- General Health & Medical Sciences (AREA)
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- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
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Abstract
The invention belongs to optical microscopy field, specially a kind of single object lens mating plate micro imaging system.Micro imaging system of the present invention includes fluorescence activating system and fluorescence gathering system；Wherein, fluorescence activating system includes: laser light source, lens combination beam-expanding system, mating plate generation device, mating plate zoom lens control device, dichroic mirror module, the excitation object lens, 45 degree of reflective micro-mirrors devices successively arranged by optical path, sample stage；Described fluorescence gathering system, including image-forming objective lens, dichroic mirror, condenser lens, camera etc..Single object lens excitation sample is used only in the present invention uses this object lens collection fluorescent to enormously simplify the microscopie unit of mating plate compared to traditional doublet mating plate microscope simultaneously, and system cost is greatly lowered and easily builds；Different multiples object lens can be used in the present invention and match different magnitude of 45 degree of reflective micro-mirrors, so that different sample sizes (in several millimeters to tens micron ranges) can be observed, there is compatibility well.
Description
Technical field
The invention belongs to optical microscopy fields, and in particular to a kind of list object lens mating plate micro imaging system.
Background technique
In contemporary optics imaging technique, the microscopical imaging mode of mating plate is that selection illumination focal plane is imaged, by
The sample of focal plane is only excited in this lighting method, so that image is no longer by the interference of defocus signal, so as to
To realize optical section tomography.Mating plate microscopy reduces the generation of defocus background signal from source, to solve
A root problem in fluorescence microscopy of having determined, i.e., after object defocus, the image that it is generated can fog, but not
It completely disappears, but reduces contrast and signal-to-noise ratio in image focal plane as background.With wide city, copolymerization coke, mostly light
The fluorescence microscopies such as son are compared, and the light of mating plate fluorescence microscopy is drifted and phototoxicity has all reached extremely low limit, are applicable in very much
In the three-dimensional imaging of relatively transparent tissue or entire organism, there is long observation time, high imaging rate and multiple observation visual angles
Body imaging etc. many advantages.
Traditional mating plate microscope is general, and there are two object lens to constitute, and one is excitation object lens, and one is to collect object lens, and two
Object lens are vertically put at an angle of 90, due to being limited by object lens operating distance itself and placing space, are exciting and collecting object lens
Selection on, the object lens for not all being available high magnification numbe high-NA are combined, can only use lower multiple object lens carry out
It excites sample and collects fluorescent image.Under the image-forming condition of low power number object lens combination, gained excites the mating plate thickness meeting of sample
It is higher, z directional image resolution ratio is limited, simultaneously because using the collection fluorescence imaging of low NA objective, fluorescent image
Lateral resolution can be lower.So general tradition mating plate microscope is suitable only for that large scale sample is imaged, such as fruit
Fly embryo, zebra fish etc..The mating plate size that traditional mating plate microscope is formed is difficult to cope with the sample of cell magnitude.
Summary of the invention
The purpose of the present invention is to overcome the shortcomings of the existing technology and insufficient, provides a kind of single object lens mating plate micro-imaging system
System.
List object lens mating plate micro imaging system provided by the invention, can form after mating plate only by an object lens through 45
Sample is excited after degree reflective micro-mirrors reflection, while carrying out the collection of fluorescent image using the object lens, to simplify traditional pair
Object lens mating plate microscopic imaging device.
The invention further relates to a kind of preparation methods of 45 degree of reflective micro-mirrors in above-mentioned single object lens mating plate microscope.
The invention further relates to the building methods of above-mentioned single object lens mating plate micro imaging system.Pass through the object of application different multiples
The mating plate that mirror is formed adapts to various sizes of sample (such as zebra fish, drosophila embryos, cell etc.).
List object lens mating plate micro imaging system provided by the invention, including fluorescence activating system and fluorescence gathering system；Its
In:
The fluorescence activating system includes: the laser light source successively arranged by optical path, a pair of of 4f lens combination beam-expanding system, mating plate
Generation device, mating plate zoom lens control device, dichroic mirror module excite object lens, 45 degree of reflective micro-mirrors devices, sample stage；Wherein:
Laser light source, for generating laser beam；
A pair of of 4f lens combination beam-expanding system for receiving the laser beam generated by the laser light source, and adjusts the laser
The spot size of light beam；
Mating plate generation device is mating plate for receiving the laser beam being emitted by the beam-expanding system, and by the laser beam reshaping；
The mating plate for receiving the mating plate generated by the mating plate generation device, and is coupled to two colors by mating plate zoom lens control device
Mirror module carries out zooming adjustment to the mating plate simultaneously；
Dichroic mirror module, for receiving by the mating plate and being reflected into object lens back focal plane；
Object lens are excited, for receiving the mating plate by dichroic mirror reflection and coupleeing 45 degree of reflective micro-mirrors for mating plate；
45 degree of reflective micro-mirrors devices, for receiving by the object lens mating plate and mating plate being reflexed to sample, to excite the sample
Generate fluorescence；
Sample stage, z-axis translate sample stage and carry out three-dimensional imaging for mobile example in a z-direction.
The fluorescence gathering system, including the image-forming objective lens successively arranged by optical path, dichroic mirror, condenser lens, camera；Its
In:
Image-forming objective lens are used to collect the fluorescence of sample generation using the same object lens；
Dichroic mirror for receiving the fluorescence as caused by the sample, and filters out the laser due to sample scattering；
Condenser lens for receiving by the filtered fluorescence of dichroic mirror, and fluorescence is focused；
Camera for receiving the fluorescence after being focused by condenser lens, and carries out imaging to collected fluorescence and shows.
Further, the laser light source can be femto-second laser or continuous light laser.
Further, the mating plate generator can be cylindrical mirror or scanning galvanometer system.
Further, the scanning galvanometer can be X-axis galvanometer and/or Y-axis galvanometer.
It further, include that exciter filter, 45 degree of low anti-high pass dichroscopes or 45 degree are low in the dichroic mirror module
Logical high anti-dichroscope, fluorescent radiation optical filter.
Further, the object lens can be 10 times of air mirrors, 60 times of air mirrors, 60 times of oil mirrors, 100 times of oil mirrors, 150 times
Oil mirror.
Further, 45 degree of reflective micro-mirrors can be 45 degree of isosceles right triangle angles of wedge, including longest side length point
Not Wei 5 millimeters, 1.4 millimeters and 40 microns three kinds of magnitudes 45 degree of isosceles right triangle angles of wedge.
Further, the sample stage is z-axis translation stage.
Further, the camera is charge-coupled device or saturation gain charge-coupled device.
The present invention is had the following advantages and beneficial effects: compared with existing mating plate microscopy
1, single object lens mating plate microscope is only used only single object lens deexcitation sample while this object lens being used to collect sample in the present invention
Fluorescence enormously simplify the microscopie unit of mating plate compared to traditional doublet mating plate microscope, system be greatly lowered
Cost, and easily build；
2, the excitation and collection of fluorescent are carried out respectively using two object lens in traditional doublet mating plate microscope, still
Due to the influence of object lens operating distance, so that two object lens can not match the object lens of high-NA, so traditional doublet
Mating plate microscope is only suitable only for observation embryo or large scale tissue biological sample, is not suitable for observation cell magnitude sample.At this
By 45 ° of reflective micro-mirrors in invention, single object lens mating plate microscope is not influenced by space length, closely can be used
The object lens of high magnification numbe high-NA.Single object lens mating plate microscope can be used to observe the biological sample of cell magnitude in the present invention
Product, such as: unicellular sample, many cells group etc.；
3, different multiples object lens can be used in single object lens mating plate microscope of the invention and matches different magnitude of 45 ° of isosceles right angles
The triangle angle of wedge, so that zebra fish sample (three to five millimeters of ranges), embryo samples (several hundred microns to one millimeter of range), mostly carefully
Born of the same parents' group's sample (tens microns to several hundred micron ranges) etc. can be observed, so single object lens mating plate in the present invention is micro-
Mirror has compatibility well for different sample sizes.
Detailed description of the invention
Fig. 1 is single microscopical structural schematic diagram of object lens mating plate of the invention.
Fig. 2 (a) is 45 degree of isosceles right triangle angles of wedge of maximum magnitude of the invention.
Fig. 2 (b) is 45 degree of isosceles right triangle angles of wedge of medium magnitude of the invention.
Fig. 2 (c) is 45 degree of isosceles right triangle angles of wedge of minimum level of the invention.
Fig. 3 (a) is detected for the embodiment of the present invention and is formed by mating plate size x-y plane figure under 10 times of object lens.
Fig. 3 (b) is detected for the embodiment of the present invention and is formed by mating plate size y-z plane figure under 10 times of object lens.
Fig. 3 (c) is that the embodiment of the present invention detects the Gaussian distribution curve figure that mating plate thickness is formed by under 10 times of object lens.
Fig. 4 (a) is detected for the embodiment of the present invention and is formed by mating plate size x-y plane figure under 60 times of object lens.
Fig. 4 (b) is detected for the embodiment of the present invention and is formed by mating plate size y-z plane figure under 60 times of object lens.
Fig. 4 (c) is that the embodiment of the present invention detects the Gaussian distribution curve figure that mating plate thickness is formed by under 60 times of object lens.
Figure label: 1 is continuous light laser or femto-second laser, 2 and 3 composition extender lens groups, 4 for cylindrical mirror or
Person's scanning galvanometer system, 5 be varifocal mirror, and 6 be exciter filter, and 7 be low anti-high pass dichroic mirror or high anti-low pass dichroic mirror, 8
It is objective table for object lens, 9,10 be 45 degree of isosceles right triangle angles of wedge, and 11 be sample, and 12 be fluorescent optical filter, and 13 be focusing
Lens, 14 be charge-coupled device (CCD).
Specific embodiment
Present invention will now be described in further detail with reference to the embodiments and the accompanying drawings, but embodiments of the present invention are unlimited
In this.
Embodiment 1
A kind of basic structure of single object lens mating plate microscopy imaging system of the present invention is as shown in Figure 1,1 be wherein continuous light laser
Or femto-second laser, 2 and 3 composition extender lens groups, 4 be cylindrical mirror or scanning galvanometer system, and 5 be varifocal mirror, and 6 be excitation
Optical filter, 7 be low anti-high pass dichroic mirror or high anti-low pass dichroic mirror, and 8 be object lens, and 9 be objective table, and 10 be 45 degree of isosceles right angles
The triangle angle of wedge, 11 be sample, and 12 be fluorescent optical filter, and 13 be condenser lens, and 14 be charge-coupled device (CCD).Micro- system
The short wavelength's continuous laser or femtosecond laser output beam that laser 1 generates when system work are that spatial light enters single object lens light
In piece microscope optical system, extender lens group 2 and 3, cylindrical mirror or scanning vibration are sequentially placed in the laser beam direction of advance
Mirror 4, varifocal mirror 5, exciter filter 6, low anti-high pass dichroscope or high anti-low pass dichroscope 7, wherein dichroscope 7 with should
It places at laser beam angle at 45 °；Perpendicular to the laser beam and pass through the short logical dichroscope 7 optical axis direction on, this two to
7 top of Look mirror has coaxially been sequentially placed object lens 8,9,45 degree of isosceles right triangle angles of wedge 10 of objective table, sample 11, at this
It is placed with fluorescent optical filter 12 below short logical dichroscope 7, imaging and focusing lens 13,14,45 degree of isosceles of charge-coupled device are straight
The angle triangle angle of wedge 10 is placed on objective table 9, while the sample for indicating dyestuff modification is placed on the focus of object lens on objective table
Place.Dichroscope 7 makes exciting light reflect and focus at object lens back focal plane, through 45 degree of isosceles right triangle wedges after object lens
Corner reflection remembers that exciting light forms mating plate excitation sample at focal plane of lens, and the sample modified by dyestuff is by laser
Excitation generates Fluoroscopic.
It can accordingly be improved in above-mentioned basic structure in actual use, it is straight for example, by using 45 degree of isosceles shown in Fig. 2
The angle triangle angle of wedge will select corresponding different size in the list object lens mating plate micro imaging system according to sample size
Object lens form mating plate and are imaged.For example, doing three-dimensional fluorescence three-dimensional imaging for the zebra fish sample of several millimeter magnitudes
When, it needs to select 10 times of object lens, needing the longest edge of matched 45 degree of isosceles right triangle angles of wedge at this time is 5 millimeters, such as Fig. 2
(a) shown in.For the drosophila embryos sample of several hundred micron dimensions, when doing three-dimensional fluorescence three-dimensional imaging, need to select 60 times
Object lens, needing the longest edge of matched 45 degree of isosceles right triangle angles of wedge at this time is 1.4 millimeters, such as Fig. 2 (b).For tens
For the cell sample of micron dimension, when doing three-dimensional fluorescence three-dimensional imaging, needs to select 100 times of oil mirrors, need at this time matched
The longest edge of 45 degree of isosceles right triangle angles of wedge is 40 microns, shown as shown in Figure 2.For 45 degree of isosceles right angle trigonometries
Two right-angle side inner walls of the shape angle of wedge do mirror surface treatment, need exclusive one layer of silver protective layer, form smooth mirror surface.Pass through this 45 degree
The mating plate generated by cylindrical mirror or scanning galvanometer is reflected on the focusing surface of object lens by mirror surface, forms wide field imaging.By not
It is also different with mating plate size is formed by after the object lens of multiple.For being formed after 45 degree of reflective micro-mirrors after 10 times of object lens
Mating plate X/Y plane dimensions length be 533 microns, width be 128 microns, as shown in Fig. 3 (a)；Mating plate with a thickness of 13.3
Micron, as shown in Figure 3 (b)；The full width at half maximum of its mating plate thickness is as shown in Figure 3 (c).For after 60 times of object lens by 45 degree
The mating plate formed after reflective micro-mirrors is 27 microns in the dimensions length of X/Y plane, and width is 11.4 microns, as shown in Figure 4 (a)；Light
Piece with a thickness of 2.5 microns, as shown in Figure 4 (b)；The full width at half maximum of its mating plate thickness is as shown in Figure 4 (c).Mating plate size is not
It is both for matching various sizes of sample, for example, mating plate size shown in Fig. 3 is suitable for making the samples such as zebra fish or drosophila embryos
Product, mating plate size shown in Fig. 4 are suitable for doing cell imaging.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention,
It should be equivalent substitute mode, be included within the scope of the present invention.
Claims (8)
1. a kind of list object lens mating plate micro imaging system, which is characterized in that be made of fluorescence activating system and fluorescence gathering system；
Wherein:
The fluorescence activating system includes:
Laser light source, for generating laser beam；
A pair of of 4f lens combination beam-expanding system for receiving the laser beam generated by the laser light source, and adjusts the laser
The spot size of light beam；
Mating plate generation device is mating plate for receiving the laser beam being emitted by the beam-expanding system, and by the laser beam reshaping；
The mating plate for receiving the mating plate generated by the mating plate generation device, and is coupled to two colors by mating plate zoom lens control device
Mirror module, while zooming adjustment is carried out to the mating plate；
Dichroic mirror module for receiving by the mating plate, and is reflected into object lens back focal plane；
Object lens are excited, for receiving the mating plate by dichroic mirror reflection and coupleeing 45 ° of reflective micro-mirrors for mating plate；
45 degree of reflective micro-mirrors devices, for receiving by the object lens mating plate and mating plate being reflexed to sample, to excite the sample
Generate fluorescence；
Sample stage, for carrying sample, while can mobile example in the z-axis direction so that sample does three-dimensional imaging；
The fluorescence gathering system includes:
Image-forming objective lens are used to collect the fluorescence of sample generation using the same object lens；
Dichroic mirror for receiving the fluorescence as caused by the sample, and filters out the laser due to sample scattering；
Condenser lens for receiving by the filtered fluorescence of dichroic mirror, and fluorescence is focused；
Charge-coupled device for receiving the fluorescence after being focused by condenser lens, and carries out imaging to collected fluorescence and shows.
2. list object lens mating plate microscopic system according to claim 1, which is characterized in that the laser is continuous ray laser
Device or femtosecond light laser.
3. list object lens mating plate microscopic system according to claim 1, which is characterized in that the mating plate generation device is cylinder
Mirror or galvanometer scanning system.
4. list object lens mating plate microscopic system according to claim 1,2 or 3, which is characterized in that the mating plate zoom lens control device
For varifocal mirror, change mating plate focal position for continuous.
5. list object lens mating plate microscopic system according to claim 4, which is characterized in that the mating plate zoom lens control device is used for
Continuous to change mating plate focal position, position is at the back focal plane of different object lens.
6. list object lens mating plate microscopic system according to claim 1,2 or 3, which is characterized in that 45 degree of reflective micro-mirrors
It is 45 degree of isosceles right triangle angles of wedge, is used for reflected excitation light, it is made to form mating plate, including longest edge at focal plane of lens
Long is respectively 45 degree of isosceles right triangle angles of wedge of 5 millimeters, 1.4 millimeters and 40 microns three kinds of magnitudes.
7. list object lens mating plate microscopic system according to claim 6, which is characterized in that the sample stage is the mobile sample of z-axis
Sample platform, the movement for sample in the direction z carry out three-dimensional imaging.
8. list object lens mating plate microscopic system according to claim 1, which is characterized in that the laser is femto-second laser
When, the mating plate wide field of formation is imaged as the imaging of the multi-photons such as two-photon, three-photon.
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| CN111580261A (en) * | 2020-07-01 | 2020-08-25 | 中国科学技术大学 | Micro-imaging device based on epi-illumination |
| CN112835190A (en) * | 2021-01-04 | 2021-05-25 | 桂林电子科技大学 | Double-core optical fiber light control and dynamic speckle illumination microscopic imaging method and system |
| CN113484297A (en) * | 2021-09-07 | 2021-10-08 | 清华大学 | Fluorescent light sheet microscope system |
| CN114965405A (en) * | 2022-05-16 | 2022-08-30 | 中国科学院生物物理研究所 | Super-resolution single-objective lens optical sheet microscopic imaging optical system and imaging system thereof |
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| CN111580261A (en) * | 2020-07-01 | 2020-08-25 | 中国科学技术大学 | Micro-imaging device based on epi-illumination |
| CN112835190A (en) * | 2021-01-04 | 2021-05-25 | 桂林电子科技大学 | Double-core optical fiber light control and dynamic speckle illumination microscopic imaging method and system |
| CN112835190B (en) * | 2021-01-04 | 2022-08-09 | 桂林电子科技大学 | Based on two core optic fibre light manipulation and dynamic speckle illumination microscopic imaging system |
| CN113484297A (en) * | 2021-09-07 | 2021-10-08 | 清华大学 | Fluorescent light sheet microscope system |
| CN113484297B (en) * | 2021-09-07 | 2021-12-24 | 清华大学 | Fluorescent light sheet microscope system |
| CN114965405A (en) * | 2022-05-16 | 2022-08-30 | 中国科学院生物物理研究所 | Super-resolution single-objective lens optical sheet microscopic imaging optical system and imaging system thereof |
| CN114965405B (en) * | 2022-05-16 | 2023-12-01 | 中国科学院生物物理研究所 | Super-resolution single-objective light sheet microscopic imaging system |
| CN115901711A (en) * | 2023-01-05 | 2023-04-04 | 浙江大学 | Method for representing three-dimensional structure information of chloroplast based on three-photon fluorescence microscopy |
| CN115901711B (en) * | 2023-01-05 | 2023-08-25 | 浙江大学 | Method for representing chloroplast three-dimensional structure information based on three-photon fluorescence microscopy |
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