CN103055973A - Novel micro-fluidic separating chip and preparation method thereof - Google Patents
Novel micro-fluidic separating chip and preparation method thereof Download PDFInfo
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- CN103055973A CN103055973A CN2012105871207A CN201210587120A CN103055973A CN 103055973 A CN103055973 A CN 103055973A CN 2012105871207 A CN2012105871207 A CN 2012105871207A CN 201210587120 A CN201210587120 A CN 201210587120A CN 103055973 A CN103055973 A CN 103055973A
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Abstract
The invention relates to a novel micro-fluidic separating chip and a preparation method thereof. A microstructure and a micro-channel are arranged on the surface of the micro-fluidic chip; an electroosmotic pump driven by a sample is prepared in situ; a certain electric field is applied to the two sides of a separating main micro-channel; under the effect of the electric field, different charged substances to be detected are separated; and the micro-fluidic separating chip is mainly applied to the related field of separation, analysis and the like. The micro-fluidic chip is simple in operation; the charged substances are simultaneously fed and separated on the chip; the consumption of reagents and samples is reduced, the replacing frequency of a capillary tube is reduced, the analysis cost of capillary electrophoresis is greatly reduced, the novel micro-fluidic separating chip provided by the invention is portable, economic, rapid and efficient, and a brand new micro-fluidic analysis technology is provided for the related field of separation, analysis and the like.
Description
Technical field
The present invention relates to a kind of new microfluidic separating chips and preparation method thereof, there are micro-structural and microchannel in this micro-fluidic chip surface, original position has prepared the electroosmotic pump that sample drives, in the both sides of separating main microchannel, apply certain electric field, under the effect of electric field, charged different test substance is realized separating, and is mainly used in the association areas such as separation and analysis.
Background technology
Capillary Electrophoresis claims again HPCE, is the Novel liquid-phase isolation technics of a class take capillary as split tunnel, take high-voltage dc as driving force.In fact Capillary Electrophoresis comprises electrophoresis, chromatogram and intersection content thereof, and it makes analytical chemistry be able to enter from the microlitre level that to receive premium on currency flat, and makes single cell analysis, and even single molecule analysis becomes possibility.Therefore the compartment analysis of long-term our large biological molecule of puzzlement such as protein has also had new favourable turn.
Capillary Electrophoresis possesses following advantage: fast, generally finished separation in tens minutes; Trace, the required sample volume of sample introduction is the nL level; Multi-mode can be selected as required different clastotypes and only need an instrument; Economy, experiment consumes only several milliliters of cushioning liquid, and standing charges are very low; Automatically, be the higher separation method of present automaticity.
Yet because sample size is few, Capillary Electrophoresis possesses following shortcoming: preparative capacibility is poor; Because capillary diameter is little, makes light path too short, during with some detection methods (such as ultraviolet absorption spectroscopy), sensitivity is lower; Electric osmose can change because of sample composition, and then impact separates reappearance.Therefore, develop a kind of convenient, quick, efficiently, isolation technics cheaply, be the active demand in the fields such as separation and analysis.In recent years, the microfluidic chip analysis technology has become important research direction in the analytical chemistry, is wherein most active one, is all to have obtained to pay attention to widely in scientific research or application.Micro-fluidic chip is as a kind of novel analysis test platform, has high flux, integrated, multiple parallel analysis, portable, easy to operate, low cost and other advantages, is applied widely in various fields.Yet, adopt micro-fluidic chip, in its surface preparation micro-structural and microchannel, integrated sample introduction and the analytical technology that is separated into one not yet have substantial breakthrough in the related application field at present.
Summary of the invention
The purpose of this invention is to provide a kind of new microfluidic separating chips and preparation method thereof, there are micro-structural and microchannel in this micro-fluidic chip surface, original position has prepared the electroosmotic pump that sample drives, in the both sides of separating main microchannel, apply certain electric field, under the effect of electric field, charged different test substance is realized separating, and is mainly used in the association areas such as separation and analysis.
For achieving the above object, the present invention adopts following operating procedure:
(1) micro-structural and the microchannel figure of each layer chip in Computer-aided design Software for Design and the drafting micro-fluidic chip.
(2) by micro-processing technology machining needs on each layer micro-fluidic chip substrate surface and adhesive membrane micro-structural and microchannel, comprise sample holes, separate the main channel and separate subchannel.
(3) capillary with three sections (1 centimetres, 150 microns of diameters) is embedded in the electroosmotic pump microflute, seals with fixing with epoxy glue.
(4) the high voltage source controller is linked to each other by platinum electrode with split tunnel with electroosmotic pump.
(5) utilize double-deck adhesive membrane, with the alignment of each layer micro-fluidic chip, bonding, pressurizing and sealing, form the micro-fluidic chip of novel capillary electrophoresis.
Among the present invention, the chip substrates of new microfluidic separating chips can be PMMA, PC, PVC, COC, copper, aluminium, stainless steel, silicon chip, glass wafer, also commercially available all kinds of common CD CD.
Among the present invention, the micro-structural of new microfluidic separating chips and adhesive membrane and microchannel can be by numerical control mill quarter, laser ablation, LIGA technology, method of molding, pressure sintering, chemical attack preparations, also available soft lithographic technique preparation.
Among the present invention, the new microfluidic separating chips is comprised of layers of chips, fits with adhesive membrane between each layer chip, and adhesive membrane can be that double-deck power causes adhesive membrane, also common double faced adhesive tape film.
Among the present invention, the EOF that the driving of the sample solution on the new microfluidic separating chips relies on electroosmotic pump to produce.
Among the present invention, both sides, the main microchannel of the separation of new microfluidic separating chips connect high voltage source, and the EOF that relies on electric field to produce carries out the separation of charge species.
New microfluidic separating chips that the present invention proposes and preparation method thereof, sample introduction simple to operate, as on chip, to have realized simultaneously charge species with separate, reduced the consumption of reagent and sample, reduced replacement frequency capillaceous, greatly reduced the analysis cost of Capillary Electrophoresis, have portable, economic, characteristics fast and efficiently, in the association areas such as separation and analysis, have a good application prospect.
Description of drawings
Fig. 1. the structural representation of new microfluidic separating chips.
A. Control of Voltage panel, the b. electroosmotic pump, c. separates the main channel, d. capillary, e. ultraviolet detection window.
Specific embodiments
Embodiment 1
Micro-structural and the microchannel figure of the layers of chips of Computer-aided design Software for Design and drafting centrifugal type microfludic chip.Utilize micro-structural and the microchannel of numerical control CNC system processing preparation two-layer polymethyl methacrylate (PMMA) chip, clean each layer chip with running water, distilled water respectively, and with spots such as the residual fingerprint of ethanol chip surface, oil stains.On the double faced adhesive tape film, with required micro-structural and the microchannel of carving machine processing preparation.The capillary of three sections (1 centimetres, 150 microns of diameters) is embedded in the electroosmotic pump microflute, seals with fixing with epoxy glue.With layers of chips carefully align, bonding, pressurizing and sealing, make the micro-fluidic chip of novel capillary electrophoresis.Charged solution is injected the sample introduction hand-hole, apply electric field, solution enters under electroosmotic pump drives and separates main microchannel, under the effect of electric field, the translational speed of the material to be separated that electric charge is different is different, thereby realizes separating, after entering detection window, be detected respectively its content.
Embodiment 2
Micro-structural and the microchannel figure of the layers of chips of Computer-aided design Software for Design and drafting centrifugal type microfludic chip.Utilize micro-structural and the microchannel of numerical control CNC system processing preparation two-layer disc-shaped Merlon (PC) chip, clean each layer chip with running water, distilled water respectively, and with spots such as the residual fingerprint of ethanol chip surface, oil stains.On the double faced adhesive tape film, with required micro-structural and the microchannel of carving machine processing preparation.The capillary of three sections (1 centimetres, 150 microns of diameters) is embedded in the electroosmotic pump microflute, seals with fixing with epoxy glue.With layers of chips carefully align, bonding, pressurizing and sealing, make the micro-fluidic chip of novel capillary electrophoresis.Charged solution is injected the sample introduction hand-hole, apply electric field, solution enters under electroosmotic pump drives and separates main microchannel, under the effect of electric field, the translational speed of the material to be separated that electric charge is different is different, thereby realizes separating, after entering detection window, be detected respectively its content.
Claims (10)
1. new microfluidic separating chips and preparation method thereof, there are micro-structural and microchannel in this micro-fluidic chip surface, original position has prepared the electroosmotic pump that sample drives, in the both sides of separating main microchannel, apply certain electric field, under the effect of electric field, charged different test substance is realized separating, and is mainly used in the association areas such as separation and analysis.
2. by new microfluidic separating chips claimed in claim 1 and preparation method thereof, it is characterized in that its making step is as follows:
(1) micro-structural and the microchannel figure of each layer chip in Computer-aided design Software for Design and the drafting micro-fluidic chip.
(2) by micro-processing technology machining needs on each layer micro-fluidic chip substrate surface and adhesive membrane micro-structural and microchannel, comprise sample holes, separate the main channel and separate subchannel.
(3) capillary with three sections (1 centimetres, 150 microns of diameters) is embedded in the electroosmotic pump microflute, seals with fixing with epoxy glue.
(4) the high voltage source controller is linked to each other by platinum electrode with split tunnel with electroosmotic pump.
(5) utilize double-deck adhesive membrane, with the alignment of each layer micro-fluidic chip, bonding, pressurizing and sealing, form the micro-fluidic chip of novel capillary electrophoresis.
3. by claim 1 or 2 described new microfluidic separating chips and preparation method thereof, it is characterized in that, the Core Feature device of this new microfluidic separating chips is micro-fluidic chip, the EOF that this chip produces with electroosmotic pump is as the driving force that mixed solution flows, and can produce in batches, repeatedly utilization, flexible design and assembling.
4. by claim 1 or 2 described new microfluidic separating chips and preparation method thereof, it is characterized in that, micro-structural on this new microfluidic separating chips and microchannel be micro-processing method by numerical control mill quarter, laser ablation, LIGA technology, method of molding, pressure sintering, chemical attack, soft lithographic technique in the preparation of chip substrates surface, size is in micron level.
5. by claim 1 or 2 described new microfluidic separating chips and preparation method thereof, it is characterized in that this new microfluidic separating chips is to be formed by stacking by layers of chips, consist of micro-structural and the microchannel network of 3 D stereo.
6. by claim 1 or 2 described new microfluidic separating chips and preparation method thereof, it is characterized in that, this new microfluidic separating chips can be made many group micro-structurals and microchannel at chip piece, consist of multicomponent from the unit, can separate simultaneously many group mixed solutions, improve the parallel separating power of unit interval.
7. by claim 1 or 2 described new microfluidic separating chips and preparation method thereof, it is characterized in that this new microfluidic separating chips is finished the separation that separates charge species in the main microchannel by applying certain electric field.
8. by claim 1 or 2 described new microfluidic separating chips and preparation method thereof, it is characterized in that this new microfluidic separating chips is integrated sample introduction and be separated into one.
9. by claim 1 or 2 described new microfluidic separating chips and preparation method thereof, it is characterized in that this new microfluidic separating chips is fit to separate fast.
10. by claim 1 or 2 described new microfluidic separating chips and preparation method thereof, it is characterized in that, this new microfluidic separating chips has portable, economic, quick, efficient, parallel separating power height, sample cross pollution probability is little, and the numerous association areas related at separation and analysis are with a wide range of applications.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103344464A (en) * | 2013-06-08 | 2013-10-09 | 南京理工大学 | Micro-fluidic agglutinin chip for glycosyl separation, and preparation method thereof |
| CN103611586A (en) * | 2013-12-06 | 2014-03-05 | 山东省科学院海洋仪器仪表研究所 | Preparation method of high-smoothness and low-deformation polymer micro-fluidic optical chip |
| CN108072592A (en) * | 2017-12-19 | 2018-05-25 | 山东交通学院 | A kind of micro-fluidic fluid dynamic viscosity detection method |
| CN108282955A (en) * | 2018-02-06 | 2018-07-13 | 苏州智能制造研究院有限公司 | A kind of modularized circuit component and preparation method thereof |
| US10300483B2 (en) | 2014-03-07 | 2019-05-28 | Capitalbio Technology Corporation | Multi-index detection microfluidic chip and methods of use |
| CN110354926A (en) * | 2019-08-27 | 2019-10-22 | 中国科学院理化技术研究所 | Electric osmose Micropump device |
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| CN1898016A (en) * | 2003-11-21 | 2007-01-17 | 株式会社荏原制作所 | Microchip device using liquid |
| CN102564925A (en) * | 2010-12-17 | 2012-07-11 | 索尼公司 | Microchip and particulate fractional collection apparatus |
| CN102784671A (en) * | 2012-08-13 | 2012-11-21 | 苏州汶颢芯片科技有限公司 | Centrifugal micro-fluidic chip for detecting pesticide residue and preparation method thereof |
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| CN1898016A (en) * | 2003-11-21 | 2007-01-17 | 株式会社荏原制作所 | Microchip device using liquid |
| CN1588033A (en) * | 2004-10-14 | 2005-03-02 | 清华大学 | Simple two-step isoelectric focusing separation analytic device |
| WO2006078968A2 (en) * | 2005-01-20 | 2006-07-27 | Brigham Young University | Integrated planar microfluidic bioanalytical systems |
| CN102564925A (en) * | 2010-12-17 | 2012-07-11 | 索尼公司 | Microchip and particulate fractional collection apparatus |
| CN102784671A (en) * | 2012-08-13 | 2012-11-21 | 苏州汶颢芯片科技有限公司 | Centrifugal micro-fluidic chip for detecting pesticide residue and preparation method thereof |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103344464A (en) * | 2013-06-08 | 2013-10-09 | 南京理工大学 | Micro-fluidic agglutinin chip for glycosyl separation, and preparation method thereof |
| CN103344464B (en) * | 2013-06-08 | 2015-07-01 | 南京理工大学 | Micro-fluidic agglutinin chip for glycosyl separation, and preparation method thereof |
| CN103611586A (en) * | 2013-12-06 | 2014-03-05 | 山东省科学院海洋仪器仪表研究所 | Preparation method of high-smoothness and low-deformation polymer micro-fluidic optical chip |
| CN103611586B (en) * | 2013-12-06 | 2015-07-15 | 山东省科学院海洋仪器仪表研究所 | Preparation method of high-smoothness and low-deformation polymer micro-fluidic optical chip |
| US10300483B2 (en) | 2014-03-07 | 2019-05-28 | Capitalbio Technology Corporation | Multi-index detection microfluidic chip and methods of use |
| CN108072592A (en) * | 2017-12-19 | 2018-05-25 | 山东交通学院 | A kind of micro-fluidic fluid dynamic viscosity detection method |
| CN108282955A (en) * | 2018-02-06 | 2018-07-13 | 苏州智能制造研究院有限公司 | A kind of modularized circuit component and preparation method thereof |
| CN110354926A (en) * | 2019-08-27 | 2019-10-22 | 中国科学院理化技术研究所 | Electric osmose Micropump device |
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Application publication date: 20130424 |