CN103055970A - Hybrid micro-fluidic chip based on micro/nano structure and preparation method thereof - Google Patents
Hybrid micro-fluidic chip based on micro/nano structure and preparation method thereof Download PDFInfo
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- CN103055970A CN103055970A CN2012105870168A CN201210587016A CN103055970A CN 103055970 A CN103055970 A CN 103055970A CN 2012105870168 A CN2012105870168 A CN 2012105870168A CN 201210587016 A CN201210587016 A CN 201210587016A CN 103055970 A CN103055970 A CN 103055970A
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Abstract
The invention relates to a hybrid micro-fluidic chip based on a micro/nano structure and a preparation method thereof. A micro-channel and the micro/nano structure are formed on the surface of the micro-fluidic chip; solution is efficiently mixed through the micro/nano structure on the surface of the micro-channel to quickly and efficiently mix micro-fluidic solution; and the hybrid micro-fluidic chip is mainly applied to the relevant fields of biological assay, phase change reaction, multi-component reaction and the like. The micro-fluidic chip and the preparation method thereof have the benefits that the operation is simple, efficient mixing of the micro-fluidic solution is realized, the mixing cost of the micro-fluidic solution is greatly reduced, the micro-fluidic chip has the characteristics of convenience in carrying, economy, quickness and high efficiency, and a brand-new analysis technology is provided for efficient and uniform mixing of the micro-fluidic solution.
Description
Technical field
The present invention relates to a kind of mixing micro-fluidic chip based on little/micro-nano structure and preparation method thereof, there are microchannel and little/micro-nano structure in this micro-fluidic chip surface, solution by on the microchannel surface little/high efficiency mixing occurs in micro-nano structure, thereby realize the mixing rapidly and efficiently of microfluid solution, be mainly used in the association areas such as biologicall test, phase transformation reaction, multi-component reaction.
Background technology
Microfluidic analysis chip has microminiaturization, automation, the advantage such as integrated, convenient and quick as a kind of novel analysis platform; obtain a wide range of applications for example synthetic screening of cell biology, analytical chemistry, environmental monitoring and protection, judicial expertise and medicine in a lot of fields.In microfluidic analysis chip, the even mixing of microfluid is the key of sample treatment and analysis, just need such operation in for example biologicall test, phase transformation reaction, the multi-component reaction, this is because the characteristics of microfluidic analysis chip will operate the microfluid under the micro-scale and control, and it is extremely small as the Fluid Volume of operation and control object, cause flow behavior and the macroscopic view of microfluid to be very different, negligible phenomenon becomes the major influence factors of Fluid Flow in A under micro-scale under macro-scale.In recent years, on microfluidic analysis chip, how to realize the even mixing to microfluid, become a research difficult problem and focus in the microfluidic analysis chip technology.
Yet, conventional hybrid technology is difficult to finish the even mixing to microfluid in the microchannel, therefore, and by the little/micro-nano structure of preparation in the microchannel of micro-fluidic chip, utilize little/micro-nano structure to the efficient mixing of solution, thereby realize evenly mixing the microfluid in the micro-fluidic chip.Develop a kind of convenient, quick, efficiently, microfluid drives and control technology cheaply, should be one of research direction of little valve on the micro-fluidic chip, and substantial breakthrough is not yet arranged at present.
Summary of the invention
The purpose of this invention is to provide a kind of mixing micro-fluidic chip based on little/micro-nano structure and preparation method thereof, there are microchannel and little/micro-nano structure in this micro-fluidic chip surface, solution by on the microchannel surface little/high efficiency mixing occurs in micro-nano structure, thereby realize the mixing rapidly and efficiently of microfluid solution, be mainly used in the association areas such as biologicall test, phase transformation reaction, multi-component reaction.
For achieving the above object, the present invention adopts following operating procedure:
(1) micro-structural and the microchannel figure of each layer chip in Computer-aided design Software for Design and the drafting micro-fluidic chip.
(2) by micro-processing technology machining needs on each layer micro-fluidic chip substrate surface and adhesive membrane micro-structural and microchannel, comprise sample holes, mix main channel and sample outlet hole.
(3) utilize double-deck adhesive membrane, with the alignment of each layer micro-fluidic chip, bonding, pressurizing and sealing, form the mobile controlled micro-fluidic chip of droplet.
Among the present invention, can be PMMA, PC, PVC, COC, copper, aluminium, stainless steel, silicon chip, glass wafer based on the micro-fluidic chip base material of the mixing micro-fluidic chip of little/micro-nano structure, also commercially available all kinds of common CD CD.
Among the present invention, can be by numerical control mill quarter, laser ablation, LIGA technology, method of molding, pressure sintering, chemical attack preparation, also available soft lithographic technique preparation based on the micro-structural of the micro-fluidic chip of the mixing micro-fluidic chip of little/micro-nano structure and adhesive membrane and microchannel.
Among the present invention, be comprised of layers of chips based on the mixing micro-fluidic chip of little/micro-nano structure, fit with adhesive membrane between each layer chip, adhesive membrane can be that double-deck power causes adhesive membrane, also common double faced adhesive tape film.
Among the present invention, based on the microchannel of the mixing micro-fluidic chip of little/micro-nano structure little/micro-nano structure is comprised of two parts, realizes respectively elementary mixing and senior mixing.
Among the present invention, based on the microchannel of the mixing micro-fluidic chip of little/micro-nano structure little/micro-nano structure comprises two parts, first is snakelike hybrid channel, second portion is the profile of tooth hybrid channel.
The present invention propose based on mixing micro-fluidic chip of little/micro-nano structure and preparation method thereof, efficient mixing simple to operate, as to have realized microfluid solution, greatly reduced the cost that microfluid solution mixes, have portable, economic, characteristics fast and efficiently, in the association areas such as biologicall test, phase transformation reaction, multi-component reaction, have a good application prospect.
Description of drawings
Fig. 1. based on the structural representation of the mixing micro-fluidic chip of little/micro-nano structure.
A. solution driving pump, b. solution driving pump, the snakelike mixed zone of c., d. profile of tooth mixed zone, e. taphole.
Specific embodiments
Embodiment 1
Micro-structural and the microchannel figure of the layers of chips of the micro-fluidic chip that Computer-aided design Software for Design and drafting microfluidic flow are controlled.Utilize micro-structural and the microchannel of numerical control CNC system processing preparation two-layer polymethyl methacrylate (PMMA) chip, clean each layer chip with running water, distilled water respectively, and with spots such as the residual fingerprint of ethanol chip surface, oil stains.On the double faced adhesive tape film, with required micro-structural and the microchannel of carving machine processing preparation.With layers of chips carefully align, bonding, pressurizing and sealing, make the intelligent little valve based on micro-fluidic chip.The sample introduction hand-hole that sample solution is added micro-fluidic chip by two peristaltic pumps, solution enters the snakelike mixed zone in the microchannel under the driving of external peristaltic pump, elementary mixing occurs, profile of tooth Mixed Zone in the microchannel, carry out senior mixing, realized that at last little/micro-nano structure is to the even mixing of microfluid solution.
Embodiment 2
Micro-structural and the microchannel figure of the layers of chips of Computer-aided design Software for Design and drafting centrifugal type microfludic chip.Utilize micro-structural and the microchannel of numerical control CNC system processing preparation two-layer disc-shaped Merlon (PC) chip, clean each layer chip with running water, distilled water respectively, and with spots such as the residual fingerprint of ethanol chip surface, oil stains.On the double faced adhesive tape film, with required micro-structural and the microchannel of carving machine processing preparation.With layers of chips carefully align, bonding, pressurizing and sealing, make the intelligent little valve based on micro-fluidic chip.The sample introduction hand-hole that sample solution is added micro-fluidic chip by two peristaltic pumps, solution enters the snakelike mixed zone in the microchannel under the driving of external peristaltic pump, elementary mixing occurs, profile of tooth Mixed Zone in the microchannel, carry out senior mixing, realized that at last little/micro-nano structure is to the even mixing of microfluid solution.
Claims (9)
1. one kind based on mixing micro-fluidic chip of little/micro-nano structure and preparation method thereof, there are microchannel and little/micro-nano structure in this micro-fluidic chip surface, solution by on the microchannel surface little/high efficiency mixing occurs in micro-nano structure, thereby realize the mixing rapidly and efficiently of microfluid solution.
2. by mixing micro-fluidic chip based on little/micro-nano structure claimed in claim 1 and preparation method thereof, it is characterized in that its making step is as follows:
(1) micro-structural and the microchannel figure of each layer chip in Computer-aided design Software for Design and the drafting micro-fluidic chip.
(2) by micro-processing technology machining needs on each layer micro-fluidic chip substrate surface and adhesive membrane micro-structural and microchannel, comprise sample holes, mix main channel and sample outlet hole.
(3) utilize double-deck adhesive membrane, with the alignment of each layer micro-fluidic chip, bonding, pressurizing and sealing, form the mobile controlled micro-fluidic chip of droplet.
3. by claim 1 or 2 described mixing micro-fluidic chips based on little/micro-nano structure and preparation method thereof, it is characterized in that, the Core Feature device of this mixing micro-fluidic chip based on little/micro-nano structure is micro-fluidic chip, and this chip can be produced in batches, repeatedly utilization, flexible design and assembling.
4. by claim 1 or 2 described mixing micro-fluidic chips based on little/micro-nano structure and preparation method thereof, it is characterized in that, the micro-structural of this mixing micro-fluidic chip based on little/micro-nano structure and microchannel be micro-processing method by numerical control mill quarter, laser ablation, LIGA technology, method of molding, pressure sintering, chemical attack, soft lithographic technique in the preparation of chip substrates surface, size is in micron level.
5. by claim 1 or 2 described mixing micro-fluidic chips based on little/micro-nano structure and preparation method thereof, it is characterized in that, this mixing micro-fluidic chip based on little/micro-nano structure is to be formed by stacking by layers of chips, consists of micro-structural and the microchannel network of 3 D stereo.
6. by claim 1 or 2 described mixing micro-fluidic chips based on little/micro-nano structure and preparation method thereof, it is characterized in that, this mixing micro-fluidic chip based on little/micro-nano structure is comprised of two groups of micro-structurals and microchannel, consists of elementary mixed cell and senior mixed cell.
7. by claim 1 or 2 described mixing micro-fluidic chips based on little/micro-nano structure and preparation method thereof, it is characterized in that this mixing micro-fluidic chip based on little/micro-nano structure efficiently mixes by the repeatedly unordered collision of solution in microchannel surface.
8. by claim 1 or 2 described mixing micro-fluidic chips based on little/micro-nano structure and preparation method thereof, it is characterized in that, this mixing micro-fluidic chip based on little/micro-nano structure can be realized the rapid mixing to solution fast, and mixing efficiency can reach 10 ml/min.
9. by claim 1 or 2 described mixing micro-fluidic chips based on little/micro-nano structure and preparation method thereof, it is characterized in that, it is portable, economical, quick, efficient that this mixing micro-fluidic chip based on little/micro-nano structure has, and is with a wide range of applications in biologicall test, phase transformation reaction, the related numerous association areas of multi-component reaction.
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103386337A (en) * | 2013-08-07 | 2013-11-13 | 苏州扬清芯片科技有限公司 | Microfluidic chip for producing multicomponent droplets |
| CN103611586A (en) * | 2013-12-06 | 2014-03-05 | 山东省科学院海洋仪器仪表研究所 | Preparation method of high-smoothness and low-deformation polymer micro-fluidic optical chip |
| CN103706413A (en) * | 2013-12-18 | 2014-04-09 | 上海交通大学 | Microfluidic phase change type bubble micro-pump valve and method thereof |
| CN105833926A (en) * | 2016-04-27 | 2016-08-10 | 浙江工业大学 | Microfluid self-driven paper base micro-fluidic chip and preparation method and application thereof |
| CN105866404A (en) * | 2016-03-31 | 2016-08-17 | 苏州市博纳泰科生物技术有限公司 | Membrane material-based micro-fluidic chip |
| CN106076446A (en) * | 2016-08-19 | 2016-11-09 | 北京工业大学 | A kind of pair of branch road realizes the microchannel of interval microlayer model fusion function |
| CN106215988A (en) * | 2016-08-19 | 2016-12-14 | 北京工业大学 | A kind of pair of branch road realizes the microchannel of twice splitting function of microlayer model |
| CN106391152A (en) * | 2016-09-20 | 2017-02-15 | 北京工业大学 | Micro-fluidic chip with deformable appointed positions of upper wall surface and lower wall surface of channel |
| CN106591105A (en) * | 2016-12-09 | 2017-04-26 | 东北大学 | Microorganism automatic sample introduction system based on microfluidic chip and preparation method thereof |
| CN109603930A (en) * | 2018-11-29 | 2019-04-12 | 上海大学 | The controllable method for preparing of liposome vesicle based on micro fluidic device |
| CN110343604A (en) * | 2019-07-15 | 2019-10-18 | 浙江大学 | A kind of residual detection chip of micro-fluidic agriculture and application |
| CN117229866A (en) * | 2023-11-13 | 2023-12-15 | 烟台张裕葡萄酿酒股份有限公司 | Method for brewing dry white wine by micro-channel mixed fermentation |
| CN117229866B (en) * | 2023-11-13 | 2024-04-12 | 烟台张裕葡萄酿酒股份有限公司 | Method for brewing dry white wine by micro-channel mixed fermentation |
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Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103386337A (en) * | 2013-08-07 | 2013-11-13 | 苏州扬清芯片科技有限公司 | Microfluidic chip for producing multicomponent droplets |
| CN103611586A (en) * | 2013-12-06 | 2014-03-05 | 山东省科学院海洋仪器仪表研究所 | Preparation method of high-smoothness and low-deformation polymer micro-fluidic optical chip |
| CN103611586B (en) * | 2013-12-06 | 2015-07-15 | 山东省科学院海洋仪器仪表研究所 | Preparation method of high-smoothness and low-deformation polymer micro-fluidic optical chip |
| CN103706413A (en) * | 2013-12-18 | 2014-04-09 | 上海交通大学 | Microfluidic phase change type bubble micro-pump valve and method thereof |
| CN105866404A (en) * | 2016-03-31 | 2016-08-17 | 苏州市博纳泰科生物技术有限公司 | Membrane material-based micro-fluidic chip |
| CN105833926B (en) * | 2016-04-27 | 2017-12-05 | 浙江工业大学 | Microfluid self-driving type paper substrate micro-fluidic chip, preparation method and applications |
| CN105833926A (en) * | 2016-04-27 | 2016-08-10 | 浙江工业大学 | Microfluid self-driven paper base micro-fluidic chip and preparation method and application thereof |
| CN106076446B (en) * | 2016-08-19 | 2018-12-07 | 北京工业大学 | A kind of double branches realize the microchannel of interval microlayer model fusion function |
| CN106215988A (en) * | 2016-08-19 | 2016-12-14 | 北京工业大学 | A kind of pair of branch road realizes the microchannel of twice splitting function of microlayer model |
| CN106076446A (en) * | 2016-08-19 | 2016-11-09 | 北京工业大学 | A kind of pair of branch road realizes the microchannel of interval microlayer model fusion function |
| CN106391152A (en) * | 2016-09-20 | 2017-02-15 | 北京工业大学 | Micro-fluidic chip with deformable appointed positions of upper wall surface and lower wall surface of channel |
| CN106391152B (en) * | 2016-09-20 | 2018-07-31 | 北京工业大学 | The two deformable micro-fluidic chips in wall surface designated position above and below channel |
| CN106591105A (en) * | 2016-12-09 | 2017-04-26 | 东北大学 | Microorganism automatic sample introduction system based on microfluidic chip and preparation method thereof |
| CN109603930A (en) * | 2018-11-29 | 2019-04-12 | 上海大学 | The controllable method for preparing of liposome vesicle based on micro fluidic device |
| CN110343604A (en) * | 2019-07-15 | 2019-10-18 | 浙江大学 | A kind of residual detection chip of micro-fluidic agriculture and application |
| CN117229866A (en) * | 2023-11-13 | 2023-12-15 | 烟台张裕葡萄酿酒股份有限公司 | Method for brewing dry white wine by micro-channel mixed fermentation |
| CN117229866B (en) * | 2023-11-13 | 2024-04-12 | 烟台张裕葡萄酿酒股份有限公司 | Method for brewing dry white wine by micro-channel mixed fermentation |
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Application publication date: 20130424 |