CN103041879A - Micro-fluidic chip for micro/nano liter quota-sampling and preparation method thereof - Google Patents
Micro-fluidic chip for micro/nano liter quota-sampling and preparation method thereof Download PDFInfo
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- CN103041879A CN103041879A CN2012105870736A CN201210587073A CN103041879A CN 103041879 A CN103041879 A CN 103041879A CN 2012105870736 A CN2012105870736 A CN 2012105870736A CN 201210587073 A CN201210587073 A CN 201210587073A CN 103041879 A CN103041879 A CN 103041879A
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Abstract
The invention relates to a micro-fluidic chip for micro/nano liter quota-sampling and a preparation method thereof. The surface of the micro-fluidic chip is provided with a micro-channel and a micro-structure; two electroosmotic pumps are manufactured in situ; after application of an electric field, quota-sampling and driving functions are achieved under the effect of electroosmotic flow, and the two electroosmotic pumps on the micro-fluidic chip are integrated, so that quota-sampling of micro/nano liter-grade micro-fluidic solutions is realized; and the micro-fluidic chip is mainly applicable to bioassay, phase-changing reaction, polycomponent reaction and related fields. The micro-fluidic chip is simple to operate, achieves the micro/nano liter-grade quota-sampling of the micro-fluidic solution, greatly lowers the cost of quota-sampling of the micro-fluidic solution, has the characteristics of economy, speediness and high efficiency, and provides a fire-new micro-sampling technique for quota-sampling of the micro-fluidic solution.
Description
Technical field
The present invention relates to a kind of little/receive micro-fluidic chip that rises quantitative sample injection and preparation method thereof, there are microchannel and micro-structural in this micro-fluidic chip surface, original position has prepared two electroosmotic pumps, after applying electric field, rely on the effect of EOF, realize quantitative sample injection and drive function, double pump unification on the micro-fluidic chip, thereby realize to little/receive the quantitative sample injection of other microfluid solution of upgrading, be mainly used in the association areas such as biologicall test, phase transformation reaction, multi-component reaction.
Background technology
Microfluidic analysis chip has microminiaturization, automation, the advantage such as integrated, convenient and quick as a kind of novel analysis platform; obtain a wide range of applications for example synthetic screening of cell biology, analytical chemistry, environmental monitoring and protection, judicial expertise and medicine in a lot of fields.In microfluidic analysis chip, the quantitative sample injection of microfluid is the key of sample treatment and analysis, just need such operation in for example biologicall test, phase transformation reaction, the multi-component reaction, this is because the characteristics of microfluidic analysis chip will operate the microfluid under the micro-scale and control, and it is extremely small as the Fluid Volume of operation and control object, cause flow behavior and the macroscopic view of microfluid to be very different, negligible phenomenon becomes the major influence factors of Fluid Flow in A under micro-scale under macro-scale.In recent years, on microfluidic analysis chip, how to realize the quantitative sample injection to microfluid, become a research difficult problem and focus in the microfluidic analysis chip technology.
Quantitative sample injection to microfluid in the micro-fluidic chip can be realized by Micropump.In recent years, along with development and the maturation of microflow control technique, research and develop a lot of methods and device, realized to a certain extent driving and control to microfluid in the micro-fluidic chip, also had some limitation simultaneously.Aspect Micropump research, comprise mechanical Micropump and on-mechanical Micropump.For example, compression pump (machinery or syringe pump) simple to operate, easy realization, with low cost, but be difficult for realizing miniaturization at chip.Hot gas power Micropump braking mechanism is simple, conditional request is low, be fit to batch production, but because heat lag, braking frequency is lower.The characteristics of mems electrostatic pump are that braking frequency is high, simple in structure, but brake force is lower.The electromagnetism Micropump has larger brake force, and operating voltage is low, but does not have advantage on the volume.The electrohydrodynamic micro-pump structure is simple, easily processing, cost are low, but requires harsh to the dielectric property of liquid stream.
Yet, conventional sampling technique is difficult to finish the quantitative sample injection to microfluid in the microchannel, driven by electroosmosis is to use the widest microfluid to drive and control technology in the present microfluidic analysis chip, have flow velocity steadily, no pulse, can realize that the liquid stream of valveless switches.Develop a kind of convenient, quick, efficiently, microfluid quantitative sample injection technology, especially electroosmotic pump quantitative sample injection should be one of research directions of Micropump on the micro-fluidic chip cheaply, and substantial breakthrough is not yet arranged at present.
Summary of the invention
The purpose of this invention is to provide a kind of little/receive micro-fluidic chip that rises quantitative sample injection and preparation method thereof, there are microchannel and micro-structural in this micro-fluidic chip surface, original position has prepared two electroosmotic pumps, after applying electric field, rely on the effect of EOF, realize quantitative sample injection and drive function, double pump unification on the micro-fluidic chip, thereby realize to little/receive the quantitative sample injection of other microfluid solution of upgrading, be mainly used in the association areas such as biologicall test, phase transformation reaction, multi-component reaction.
For achieving the above object, the present invention adopts following operating procedure:
(1) micro-structural and the microchannel figure of each layer chip in Computer-aided design Software for Design and the drafting micro-fluidic chip.
(2) by micro-processing technology machining needs on each layer micro-fluidic chip substrate surface and adhesive membrane micro-structural and microchannel, comprise electroosmotic pump microchannel, sample holes, sample channel and sample outlet hole.
(3) common capillary is imbedded the electroosmotic pump microchannel, be fixed and seal with epoxy resin.
(4) utilize double-deck adhesive membrane, with the alignment of each layer micro-fluidic chip, bonding, pressurizing and sealing, form the micro-fluidic chip of quantitative sample injection.
Among the present invention, little/base material of the micro-fluidic chip that rises quantitative sample injection received can be PMMA, PC, PVC, COC, copper, aluminium, stainless steel, silicon chip, glass wafer, also commercially available all kinds of common CD CD.
Among the present invention, little/receiving the micro-structural of the micro-fluidic chip of the micro-fluidic chip that rises quantitative sample injection and adhesive membrane and microchannel can be by numerical control mill quarter, laser ablation, LIGA technology, method of molding, pressure sintering, chemical attack preparation, also available soft lithographic technique preparation.
Among the present invention, the little/micro-fluidic chip that rises quantitative sample injection of receiving is comprised of layers of chips, fits with adhesive membrane between each layer chip, and adhesive membrane can be that double-deck power causes adhesive membrane, also common double faced adhesive tape film.
Among the present invention, little/receive the micro-fluidic chip that rises quantitative sample injection the microchannel little/micro-nano structure is comprised of electroosmotic pump microchannel and sample introduction microchannel two parts.
Among the present invention, little/electroosmotic pump of microchannel of the micro-fluidic chip that rises quantitative sample injection received comprises the sample introduction electroosmotic pump and drives two parts of electroosmotic pump, and first realizes quantitative sample injection, and second portion realizes that sample drives.
That the present invention proposes is a kind of little/receive micro-fluidic chip that rises quantitative sample injection and preparation method thereof, simple to operate, realized microfluid solution little/receive the upgrading other quantitative sample injection, greatly reduced the cost of microfluid solution quantitative sample injection, have portable, economic, characteristics fast and efficiently, in the association areas such as biologicall test, phase transformation reaction, multi-component reaction, have a good application prospect.
Description of drawings
Fig. 1. a kind of little/receive the structural representation of the micro-fluidic chip that rises quantitative sample injection.
A. driving pump, b. sampling pump, c. solution cushion hole, d. sample channel, e. taphole.
Specific embodiments
Embodiment 1
Micro-structural and the microchannel figure of the layers of chips of the micro-fluidic chip that Computer-aided design Software for Design and drafting microfluidic flow are controlled.Utilize micro-structural and the microchannel of numerical control CNC system processing preparation two-layer polymethyl methacrylate (PMMA) chip, clean each layer chip with running water, distilled water respectively, and with spots such as the residual fingerprint of ethanol chip surface, oil stains.On the double faced adhesive tape film, with required micro-structural and the microchannel of carving machine processing preparation.Common capillary is imbedded the electroosmotic pump microchannel, be fixed and seal with epoxy resin.With layers of chips carefully align, bonding, pressurizing and sealing, make the micro-fluidic chip of quantitative sample injection.The sample introduction hand-hole that sample solution is added micro-fluidic chip by the sample introduction electroosmotic pump, control voltage and sample injection time, after reaching volume required solution, under the driving that drives electroosmotic pump, quantitative solution enters in the microchannel, realized at last little/receive the upgrading other microfluid solution quantitative sample injection.
Embodiment 2
Micro-structural and the microchannel figure of the layers of chips of Computer-aided design Software for Design and drafting centrifugal type microfludic chip.Utilize micro-structural and the microchannel of numerical control CNC system processing preparation two-layer disc-shaped Merlon (PC) chip, clean each layer chip with running water, distilled water respectively, and with spots such as the residual fingerprint of ethanol chip surface, oil stains.On the double faced adhesive tape film, with required micro-structural and the microchannel of carving machine processing preparation.Common capillary is imbedded the electroosmotic pump microchannel, be fixed and seal with epoxy resin.With layers of chips carefully align, bonding, pressurizing and sealing, make the micro-fluidic chip of quantitative sample injection.The sample introduction hand-hole that sample solution is added micro-fluidic chip by the sample introduction electroosmotic pump, control voltage and sample injection time, after reaching volume required solution, under the driving that drives electroosmotic pump, quantitative solution enters in the microchannel, realized at last little/receive the upgrading other microfluid solution quantitative sample injection.
Claims (9)
- One kind little/receive micro-fluidic chip that rises quantitative sample injection and preparation method thereof, there are microchannel and micro-structural in this micro-fluidic chip surface, original position has prepared two electroosmotic pumps, after applying electric field, rely on the effect of EOF, realize quantitative sample injection and drive function, double pump unification on the micro-fluidic chip, thereby realize to little/receive the quantitative sample injection of other microfluid solution of upgrading.
- By claimed in claim 1 little/receive micro-fluidic chip that rises quantitative sample injection and preparation method thereof, it is characterized in that its making step is as follows:(1) micro-structural and the microchannel figure of each layer chip in Computer-aided design Software for Design and the drafting micro-fluidic chip.(2) by micro-processing technology machining needs on each layer micro-fluidic chip substrate surface and adhesive membrane micro-structural and microchannel, comprise electroosmotic pump microchannel, sample holes, sample channel and sample outlet hole.(3) common capillary is imbedded the electroosmotic pump microchannel, be fixed and seal with epoxy resin.(4) utilize double-deck adhesive membrane, with the alignment of each layer micro-fluidic chip, bonding, pressurizing and sealing, form the micro-fluidic chip of quantitative sample injection.
- By claim 1 or 2 described little/receive micro-fluidic chip that rises quantitative sample injection and preparation method thereof, it is characterized in that, this little/the Core Feature device of the micro-fluidic chip that rises quantitative sample injection received is micro-fluidic chip, this chip can be produced in batches, repeatedly utilization, flexible design and assembling.
- By claim 1 or 2 described little/receive micro-fluidic chip that rises quantitative sample injection and preparation method thereof, it is characterized in that, this little/receive the micro-structural of the micro-fluidic chip that rises quantitative sample injection and microchannel be micro-processing method by numerical control mill quarter, laser ablation, LIGA technology, method of molding, pressure sintering, chemical attack, soft lithographic technique in the preparation of chip substrates surface, size is in micron level.
- By claim 1 or 2 described little/receive micro-fluidic chip that rises quantitative sample injection and preparation method thereof, it is characterized in that, this little/micro-fluidic chip that rises quantitative sample injection of receiving is to be formed by stacking by layers of chips, consists of micro-structural and the microchannel network of 3 D stereo.
- By claim 1 or 2 described little/receive micro-fluidic chip that rises quantitative sample injection and preparation method thereof, it is characterized in that, this little/micro-fluidic chip that rises quantitative sample injection of receiving is comprised of two groups of micro-structurals and microchannel, consists of solution sample injection unit and flow of solution unit.
- By claim 1 or 2 described little/receive micro-fluidic chip that rises quantitative sample injection and preparation method thereof, it is characterized in that, this little/micro-fluidic chip that rises quantitative sample injection of receiving is used in conjunction by two electroosmotic pumps, realizes that quantitative sample injection and solution drive.
- By claim 1 or 2 described little/receive micro-fluidic chip that rises quantitative sample injection and preparation method thereof, it is characterized in that, this little/micro-fluidic chip that rises quantitative sample injection received can realize the quantitative sample injection to solution fast, the sample size of solution can or received upgrading not in the microlitre rank.
- By claim 1 or 2 described little/receive micro-fluidic chip that rises quantitative sample injection and preparation method thereof, it is characterized in that, this little/micro-fluidic chip that rises quantitative sample injection received has portablely, economical, quick, efficient, be with a wide range of applications in biologicall test, phase transformation reaction, the related numerous association areas of multi-component reaction.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103316723A (en) * | 2013-05-27 | 2013-09-25 | 苏州扬清芯片科技有限公司 | Microfluidic electrophoresis chip |
| CN104549590A (en) * | 2015-01-26 | 2015-04-29 | 北京工业大学 | Technology for modifying inner wall of micro-channel in metal micro-fluidic chip |
| CN105833926A (en) * | 2016-04-27 | 2016-08-10 | 浙江工业大学 | Microfluid self-driven paper base micro-fluidic chip and preparation method and application thereof |
| CN110479391A (en) * | 2019-08-05 | 2019-11-22 | 湖北医药学院 | A kind of low-voltage high-performance electric osmose Micropump chip based on solid-state track etching nano-pore |
| CN110787848A (en) * | 2019-11-13 | 2020-02-14 | 南京理工大学 | Micro-fluidic sampling system |
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| US20040208751A1 (en) * | 2001-05-22 | 2004-10-21 | Lazar Juliana M | Microchip integrated multi-channel electroosmotic pumping system |
| CN1588033A (en) * | 2004-10-14 | 2005-03-02 | 清华大学 | Simple two-step isoelectric focusing separation analytic device |
| TW200534916A (en) * | 2004-04-20 | 2005-11-01 | Univ Nat Sun Yat Sen | A microfluidic mixer utilizing electroosmotic flow |
| CN102331419A (en) * | 2011-03-25 | 2012-01-25 | 上海汶昌芯片科技有限公司 | Heavy metal ion detection chip and preparation method |
| CN102784671A (en) * | 2012-08-13 | 2012-11-21 | 苏州汶颢芯片科技有限公司 | Centrifugal micro-fluidic chip for detecting pesticide residue and preparation method thereof |
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2012
- 2012-12-31 CN CN2012105870736A patent/CN103041879A/en active Pending
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| US20040208751A1 (en) * | 2001-05-22 | 2004-10-21 | Lazar Juliana M | Microchip integrated multi-channel electroosmotic pumping system |
| TW200534916A (en) * | 2004-04-20 | 2005-11-01 | Univ Nat Sun Yat Sen | A microfluidic mixer utilizing electroosmotic flow |
| CN1588033A (en) * | 2004-10-14 | 2005-03-02 | 清华大学 | Simple two-step isoelectric focusing separation analytic device |
| CN102331419A (en) * | 2011-03-25 | 2012-01-25 | 上海汶昌芯片科技有限公司 | Heavy metal ion detection chip and preparation method |
| CN102784671A (en) * | 2012-08-13 | 2012-11-21 | 苏州汶颢芯片科技有限公司 | Centrifugal micro-fluidic chip for detecting pesticide residue and preparation method thereof |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103316723A (en) * | 2013-05-27 | 2013-09-25 | 苏州扬清芯片科技有限公司 | Microfluidic electrophoresis chip |
| CN104549590A (en) * | 2015-01-26 | 2015-04-29 | 北京工业大学 | Technology for modifying inner wall of micro-channel in metal micro-fluidic chip |
| CN105833926A (en) * | 2016-04-27 | 2016-08-10 | 浙江工业大学 | Microfluid self-driven paper base micro-fluidic chip and preparation method and application thereof |
| CN105833926B (en) * | 2016-04-27 | 2017-12-05 | 浙江工业大学 | Microfluid self-driving type paper substrate micro-fluidic chip, preparation method and applications |
| CN110479391A (en) * | 2019-08-05 | 2019-11-22 | 湖北医药学院 | A kind of low-voltage high-performance electric osmose Micropump chip based on solid-state track etching nano-pore |
| CN110787848A (en) * | 2019-11-13 | 2020-02-14 | 南京理工大学 | Micro-fluidic sampling system |
| CN110787848B (en) * | 2019-11-13 | 2021-05-04 | 南京理工大学 | Micro-fluidic sampling system |
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Application publication date: 20130417 |