CN103075572A - Micro-fluidic chip-based pH (Potential of Hydrogen) response micro valve and preparation method thereof - Google Patents
Micro-fluidic chip-based pH (Potential of Hydrogen) response micro valve and preparation method thereof Download PDFInfo
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- CN103075572A CN103075572A CN201210586900XA CN201210586900A CN103075572A CN 103075572 A CN103075572 A CN 103075572A CN 201210586900X A CN201210586900X A CN 201210586900XA CN 201210586900 A CN201210586900 A CN 201210586900A CN 103075572 A CN103075572 A CN 103075572A
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Abstract
The invention relates to a micro-fluidic chip-based pH (Potential of Hydrogen) response micro valve and a preparation method thereof. A micro-structure and a micro-passage are arranged on the surface of a micro-fluidic chip; the main micro-passage is provided with a hydrophilic/hydrophobic gate controlled switch; the surface of the micro-passage is modified through pH responsive molecules; change in surface wettability is caused by reversible changes of surface topography and chemical configuration of the micro-passage under stimulation of different pHs; and motion of micro-fluid on the surface of the micro-passage is controlled, so that an intelligent micro-valve for controlling the flow of micro-fluid is realized. The valve is mainly applied to the related fields such as electrophoretic separation, chromatographic separation and immunoassay. The micro-fluidic chip realizes intelligent driving and control of the micro-fluid, the micro-fluid driving and control cost is greatly reduced, the valve has the characteristics of portability, economy, quickness and high efficiency, and a completely new analysis technology is provided for micro-fluid driving and control.
Description
Technical field
The present invention relates to a kind of pH based on micro-fluidic chip and respond little valve and preparation method thereof, there are microstructure and micro passage in this micro-fluidic chip surface, main micro passage is provided with the hydrophilic/hydrophobic door controlled switch, by pH responsiveness molecule microchannel surface is carried out modification, under different pH stimulate, the surface topography of micro passage and the reversibility of chemical structure change the variation that causes surface wettability, control liquid fluid is in the motion of microchannel surface, thereby realize intelligent little valve of the control of microfluidic flow is mainly used in electrophoretic separation, chromatographic isolation, the association areas such as immunoassay.
Background technique
Microfluidic analysis chip has microminiaturization, automation, the advantage such as integrated, convenient and quick as a kind of novel analysis platform; obtain a wide range of applications for example synthetic screening of cell biology, analytical chemistry, environmental monitoring and protection, judicial expertise and medicine in a lot of fields.In microfluidic analysis chip, the accurate sample introduction of Micro-volume liquid is the key of Sample handling and analysis, just need such operation in for example micro-fluid control chip electrophoretic separation, chromatographic isolation, the immunoassay, this is because the characteristics of microfluidic analysis chip will operate the microfluid under the micro-scale and control, and it is extremely small as the Fluid Volume of operation and control object, cause flow characteristic and the macroscopic view of microfluid to be very different, negligible phenomenon becomes the major influence factors of Fluid Flow in A under micro-scale under macro-scale.In recent years, on microfluidic analysis chip, how to realize driving and control to microfluid, become a research difficult problem and focus in the microfluidic analysis chip technology.
Driving and control to microfluid in the micro-fluidic chip can realize by little valve.In recent years, along with development and the maturation of microflow control technique, research and develop a lot of methods and device, realized to a certain extent driving and control to microfluid in the micro-fluidic chip, also had some narrow limitation simultaneously.Aspect little valve research, according to whether dynamic driving mechanism is divided into active and passive valves, the little valve of active valve such as electromagnetism, the little valve of static, the little valve of marmem, piezoelectric micromotor valve, hot pneumatic micro valve etc., the little valve of this class can be realized the ON/OFF of valve, braking ability is good, seal is high, but that shortcoming is complex structure, volume is large, be difficult to realize integrated on the chip.Passive valves does not need the external impetus braking, relies on the pressure difference of valve both sides to realize switch, its small volume, but can not initiatively carry out ON/OFF or the switching of valve.
Yet, routine techniques is difficult to finish intelligent drives and the control to microfluid in the micro passage, therefore, thereby the variation by regulation and control pH comes the infiltrating variation of control surface to drive and the control micro-fluidic chip in microfluid, it is a kind of convenient, quick, efficiently, microfluid drives and control technique cheaply to develop, should be one of research direction of little valve on the micro-fluidic chip, substantial breakthrough is not yet arranged at present.
Summary of the invention
The purpose of this invention is to provide a kind of pH based on micro-fluidic chip and responded little valve and preparation method thereof, there are microstructure and micro passage in this micro-fluidic chip surface, main micro passage is provided with the hydrophilic/hydrophobic door controlled switch, by pH responsiveness molecule microchannel surface is carried out modification, under different pH stimulate, the surface topography of micro passage and the reversibility of chemical structure change the variation that causes surface wettability, control liquid fluid is in the motion of microchannel surface, thereby realize intelligent little valve of the control of microfluidic flow is mainly used in electrophoretic separation, chromatographic isolation, the association areas such as immunoassay.
For achieving the above object, the present invention adopts following operating procedure:
(1) microstructure and the micro passage figure of each layer chip in Computer-aided design Software for Design and the drafting micro-fluidic chip.
(2) by micro-processing technology machining needs on each layer micro-fluidic chip substrate surface and adhesive membrane microstructure and micro passage, comprise sample holes, separate the main passage and separate subchannel.
(3) utilize double-deck adhesive membrane, with the alignment of each layer micro-fluidic chip, bonding, pressurizing and sealing, form the mobile controlled micro-fluidic chip of droplet.
(4) carry out pH responsiveness molecule in the micro passage to the surface modification of micro passage.
(5) the pH value of switching solution in microfluidic channel, the wellability of microchannel surface is from hydrophilic to hydrophobic adjustable.
Among the present invention, the chip substrates of the micro-fluidic chip that microfluidic flow is controlled can be PMMA, PC, PVC, COC, copper, aluminium, stainless steel, silicon chip, glass wafer, also commercially available all kinds of common CD CD.
Among the present invention, responding the microstructure of the micro-fluidic chip of little valve and adhesive membrane and micro passage based on the pH of micro-fluidic chip can be by numerical control mill quarter, laser ablation, LIGA technology, method of molding, hot-pressing method, chemical corrosion preparation, also available soft lithographic technique preparation.
Among the present invention, the micro-fluidic chip that responds little valve based on the pH of micro-fluidic chip is comprised of layers of chips, fits with adhesive membrane between each layer chip, and adhesive membrane can be that double-deck power causes adhesive membrane, also common two sided plaster film.
Among the present invention, Finishing is carried out with pH responsiveness molecule in the micro passage that responds the micro-fluidic chip of little valve based on the pH of micro-fluidic chip.
Among the present invention, the pH responsiveness molecule that responds the micro-fluidic chip of little valve based on the pH of micro-fluidic chip produces hydrophilic and hydrophobic variation at different pH.
The pH based on micro-fluidic chip that the present invention proposes responds little valve and preparation method thereof, intelligent drives and control simple to operate, as to have realized microfluid, greatly reduced the cost that microfluid drives and controls, have portable, economic, characteristics fast and efficiently, in the association areas such as electrophoretic separation, chromatographic isolation, immunoassay, have a good application prospect.
Description of drawings
Fig. 1. respond the structural representation of the micro-fluidic chip of little valve based on the pH of micro-fluidic chip.
A. solution entrance, b. microfluidic control passage (little valve), c.pH responds hydrophilic modified regions, d.pH response hydrophobic modification zone, e. taphole.
Specific embodiments
Embodiment 1
Microstructure and the micro passage figure of the layers of chips of the micro-fluidic chip that Computer-aided design Software for Design and drafting microfluidic flow are controlled.Utilize microstructure and the micro passage of numerical control CNC system processing preparation two-layer polymethylmethacrylate (PMMA) chip, clean each layer chip with tap water, distilled water respectively, and with spots such as the residual fingerprint of ethanol chip surface, oil stains.On the two sided plaster film, with required microstructure and the micro passage of carving machine processing preparation.With layers of chips carefully align, bonding, pressurizing and sealing, make the intelligent little valve based on micro-fluidic chip.The sample introduction hand-hole that sample solution is added micro-fluidic chip, solution enters in the micro passage under the driving of external peristaltic pump, little valve zone in the micro passage, the surface responds the pH value of solution, obtain hydrophilic ON/OFF and hydrophobic open/close switching, realized that at last little valve is to the intelligent control of microfluidic flow.
Embodiment 2
Microstructure and the micro passage figure of the layers of chips of Computer-aided design Software for Design and drafting centrifugal type microfludic chip.Utilize microstructure and the micro passage of numerical control CNC system processing preparation two-layer disc-shaped polycarbonate (PC) (PC) chip, clean each layer chip with tap water, distilled water respectively, and with spots such as the residual fingerprint of ethanol chip surface, oil stains.On the two sided plaster film, with required microstructure and the micro passage of carving machine processing preparation.With layers of chips carefully align, bonding, pressurizing and sealing, make the intelligent little valve based on micro-fluidic chip.The sample introduction hand-hole that sample solution is added micro-fluidic chip, solution enters in the micro passage under the driving of external peristaltic pump, little valve zone in the micro passage, the surface responds the pH value of solution, obtain hydrophilic ON/OFF and hydrophobic open/close switching, realized that at last little valve is to the intelligent control of microfluidic flow.
Claims (10)
1. the pH based on micro-fluidic chip responds little valve and preparation method thereof, there are microstructure and micro passage in this micro-fluidic chip surface, main micro passage is provided with the hydrophilic/hydrophobic door controlled switch, by pH responsiveness molecule microchannel surface is carried out modification, under different pH stimulate, the surface topography of micro passage and the reversibility of chemical structure change the variation that causes surface wettability, and control liquid fluid is in the motion of microchannel surface, thereby realization is to intelligent little valve of the control of microfluidic flow.
2. respond little valve and preparation method thereof by the pH based on micro-fluidic chip claimed in claim 1, it is characterized in that, its making step is as follows:
(1) microstructure and the micro passage figure of each layer chip in Computer-aided design Software for Design and the drafting micro-fluidic chip.
(2) by micro-processing technology machining needs on each layer micro-fluidic chip substrate surface and adhesive membrane microstructure and micro passage, comprise sample holes, separate the main passage and separate subchannel.
(3) utilize double-deck adhesive membrane, with the alignment of each layer micro-fluidic chip, bonding, pressurizing and sealing, form the mobile controlled micro-fluidic chip of droplet.
(4) carry out pH responsiveness molecule in the micro passage to the surface modification of micro passage.
(5) the pH value of switching solution in microfluidic channel, the wellability of microchannel surface is from hydrophilic to hydrophobic adjustable.
3. respond little valve and preparation method thereof by claim 1 or 2 described pH based on micro-fluidic chip, it is characterized in that, the Core Feature device that this pH based on micro-fluidic chip responds little valve is micro-fluidic chip, and this chip can be produced in batches, repeatedly utilization, flexible design and assembling.
4. respond little valve and preparation method thereof by claim 1 or 2 described pH based on micro-fluidic chip, it is characterized in that, this pH based on micro-fluidic chip respond the microstructure of little valve and micro passage be micro-processing method by numerical control mill quarter, laser ablation, LIGA technology, method of molding, hot-pressing method, chemical corrosion, soft lithographic technique in the preparation of chip substrates surface, size is in micron level.
5. respond little valve and preparation method thereof by claim 1 or 2 described pH based on micro-fluidic chip, it is characterized in that it is to be formed by stacking by layers of chips that this pH based on micro-fluidic chip responds little valve, consist of microstructure and the micro passage network of 3 D stereo.
6. respond little valve and preparation method thereof by claim 1 or 2 described pH based on micro-fluidic chip, it is characterized in that, this pH based on micro-fluidic chip responds little valve can make many group microstructures and micro passage at chip piece, consist of many group control units, the flow direction of alternative control microfluid.
7. respond little valve and preparation method thereof by claim 1 or 2 described pH based on micro-fluidic chip, it is characterized in that, this pH based on micro-fluidic chip responds little valve has carried out pH responsiveness molecule in microchannel surface Finishing.
8. respond little valve and preparation method thereof by claim 1 or 2 described pH based on micro-fluidic chip, it is characterized in that, this pH based on micro-fluidic chip responds little valve by the automatic identification of responsiveness molecule, carries out hydrophilic ON/OFF and the regulation and control of hydrophobic ON/OFF in microchannel surface.
9. respond little valve and preparation method thereof by claim 1 or 2 described pH based on micro-fluidic chip, it is characterized in that, this pH based on micro-fluidic chip responds little valve can realize ON/OFF to solution fast.
10. respond little valve and preparation method thereof by claim 1 or 2 described pH based on micro-fluidic chip, it is characterized in that, this pH based on micro-fluidic chip responds little valve and has portablely, economical, quick, efficient, is with a wide range of applications in electrophoretic separation, chromatographic isolation, the related numerous association areas of immunoassay.
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| CN104571152A (en) * | 2015-01-20 | 2015-04-29 | 哈尔滨工业大学 | Liquid drop micro fluid control closed-loop regulation device based on microvalve |
| CN108025904A (en) * | 2015-06-12 | 2018-05-11 | 芯易诊有限公司 | Element of fluid and fluid cartridge could for multiple analyte analysis |
| CN108854854A (en) * | 2017-05-16 | 2018-11-23 | 厦门大学 | A kind of functional fluid door control system |
| CN110013891A (en) * | 2019-01-24 | 2019-07-16 | 厦门大学 | A kind of microfluidic system and control method based on bionical responsiveness liquid gate |
| CN112326100A (en) * | 2020-10-28 | 2021-02-05 | 吉林大学 | Fluid pressure sensor based on micro-nano structure array surface and preparation method thereof |
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| CN104571152A (en) * | 2015-01-20 | 2015-04-29 | 哈尔滨工业大学 | Liquid drop micro fluid control closed-loop regulation device based on microvalve |
| CN108025904A (en) * | 2015-06-12 | 2018-05-11 | 芯易诊有限公司 | Element of fluid and fluid cartridge could for multiple analyte analysis |
| CN113791203B (en) * | 2015-06-12 | 2024-05-17 | 芯易诊有限公司 | Method for analyzing biological samples |
| CN113791203A (en) * | 2015-06-12 | 2021-12-14 | 芯易诊有限公司 | Method for analyzing biological samples |
| CN113791202A (en) * | 2015-06-12 | 2021-12-14 | 芯易诊有限公司 | Fluidic device for analyzing a fluidic sample and method for analyzing a biological sample |
| CN108025904B (en) * | 2015-06-12 | 2021-10-15 | 芯易诊有限公司 | Fluid cell and fluid cartridge for multi-analyte analysis |
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| CN108854854A (en) * | 2017-05-16 | 2018-11-23 | 厦门大学 | A kind of functional fluid door control system |
| CN110013891B (en) * | 2019-01-24 | 2020-09-25 | 厦门大学 | Microfluidic system based on bionic responsive liquid gating and control method |
| CN110013891A (en) * | 2019-01-24 | 2019-07-16 | 厦门大学 | A kind of microfluidic system and control method based on bionical responsiveness liquid gate |
| CN112326100A (en) * | 2020-10-28 | 2021-02-05 | 吉林大学 | Fluid pressure sensor based on micro-nano structure array surface and preparation method thereof |
| CN112326100B (en) * | 2020-10-28 | 2021-12-07 | 吉林大学 | Fluid pressure sensor based on micro-nano structure array surface and preparation method thereof |
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Application publication date: 20130501 |