CN103055975A - Micro-fluid self-driven micro-fluidic chip with temperature response and preparation method thereof - Google Patents
Micro-fluid self-driven micro-fluidic chip with temperature response and preparation method thereof Download PDFInfo
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- CN103055975A CN103055975A CN2012105871508A CN201210587150A CN103055975A CN 103055975 A CN103055975 A CN 103055975A CN 2012105871508 A CN2012105871508 A CN 2012105871508A CN 201210587150 A CN201210587150 A CN 201210587150A CN 103055975 A CN103055975 A CN 103055975A
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Abstract
The invention relates to a micro-fluid self-driven micro-fluidic chip with temperature response and a preparation method thereof. A microstructure and a micro-channel are arranged on the surface of the micro-fluidic chip; the surface of the micro-channel is modified through a temperature response molecule; when the micro-channel is stimulated by an external field temperature, the variation of surface wettability is caused by reversible variations of surface topography and chemical configuration of the micro-channel; the micro-fluid is controlled to move from the bottom up on the surface of the micro-channel, so that the micro-fluid automatically flows; and the micro-fluid self-driven micro-fluidic chip is mainly applied to the related field of electrophoretic separation, chromatographic separation, immunoassay, reaction and the like. The micro-fluidic chip is simply operated, the micro-fluid is self-driven from the bottom up, the micro-fluid driving cost is greatly reduced, the micro-fluid self-driven micro-fluidic chip is portable, economic, rapid and efficient, and a brand new micro-fluid flowing control technology is provided for micro-fluid undriven flowing.
Description
Technical field
The present invention relates to self-driven micro-fluidic chip of microfluid of a kind of temperature-responsive and preparation method thereof, there are micro-structural and microchannel in this micro-fluidic chip surface, by the temperature-responsive molecule microchannel surface is carried out modification, under the thermal stimulus of outfield, the surface topography of microchannel and the invertibity of chemical structure change the variation that causes surface wettability, the control microfluid is in the from the bottom up motion of microchannel surface, thereby realize the spontaneous mobile of microfluid, be mainly used in the association areas such as electrophoretic separation, chromatographic isolation, immunoassay, reaction.
Background technology
The innovative analysis measuring technology has played extremely important impetus to global science and technology and economic development on the history of human development.Microfluidic analysis chip has microminiaturization, automation, the advantage such as integrated, convenient and quick as a kind of novel analysis platform; obtain a wide range of applications for example synthetic screening of cell biology, analytical chemistry, environmental monitoring and protection, judicial expertise and medicine in a lot of fields.In microfluidic analysis chip, the accurate sample introduction of micro liquid is the key of sample treatment and analysis, just need such operation in for example micro-fluid control chip electrophoretic separation, chromatographic isolation, the immunoassay, this is because the characteristics of microfluidic analysis chip will operate the microfluid under the micro-scale and control, and it is extremely small as the Fluid Volume of operation and control object, cause flow behavior and the macroscopic view of microfluid to be very different, negligible phenomenon becomes the major influence factors of Fluid Flow in A under micro-scale under macro-scale.In recent years, on microfluidic analysis chip, how to realize driving and control to microfluid, become a research difficult problem and focus in the microfluidic analysis chip technology.
Driving and control to microfluid in the micro-fluidic chip can realize by Micropump.In recent years, along with development and the maturation of microflow control technique, research and develop a lot of methods and device, realized to a certain extent the driving to microfluid in the micro-fluidic chip, also had some limitation simultaneously.Aspect Micropump research, comprise mechanical Micropump and on-mechanical Micropump.For example, compression pump (machinery or syringe pump) simple to operate, easy realization, with low cost, but be difficult for realizing miniaturization at chip.Hot gas power Micropump braking mechanism is simple, conditional request is low, be fit to batch production, but because heat lag, braking frequency is lower.The characteristics of mems electrostatic pump are that braking frequency is high, simple in structure, but brake force is lower.The electromagnetism Micropump has larger brake force, and operating voltage is low, but does not have advantage on the volume.The electrohydrodynamic micro-pump structure is simple, easily processing, cost are low, but requires harsh to the dielectric property of liquid stream.Driven by electroosmosis is to use the widest microfluid to drive and control technology in the present microfluidic analysis chip, have flow velocity steadily, no pulse, can realize that the liquid stream of valveless switches, but require material surface energy that electric charge is provided, high voltage source is difficult for microminiaturized, and has heating and air bubble problem.At present, the research drop becomes study hotspot in wellability and the motion of the surface of solids, carry out modification by External field response molecule and self assembly molecule layer effects on surface, under the outfield stimulates, the invertibity of surface topography and chemical structure changes the variation that causes surface wettability, thereby the control drop is in wet face state and the motion on surface.Therefore, drive and control microfluid in the micro-fluidic chip by the infiltrating variation of control surface, because being one of research direction of Micropump on the micro-fluidic chip.
Yet, routine techniques is difficult to finish intelligent drives and the control to microfluid in the microchannel, therefore, drive microfluid in the micro-fluidic chip by the infiltrating variation of control surface, develop a kind of convenient, quick, efficiently, microfluid actuation techniques cheaply, should be how to control one of research direction of microfluid on the micro-fluidic chip, substantial breakthrough is not yet arranged at present.
Summary of the invention
The purpose of this invention is to provide self-driven micro-fluidic chip of microfluid of a kind of temperature-responsive and preparation method thereof, there are micro-structural and microchannel in this micro-fluidic chip surface, by the temperature-responsive molecule microchannel surface is carried out modification, under the thermal stimulus of outfield, the surface topography of microchannel and the invertibity of chemical structure change the variation that causes surface wettability, the control microfluid is in the from the bottom up motion of microchannel surface, thereby realize the spontaneous mobile of microfluid, be mainly used in the association areas such as electrophoretic separation, chromatographic isolation, immunoassay, reaction.
For achieving the above object, the present invention adopts following operating procedure:
(1) micro-structural and the microchannel figure of each layer chip in Computer-aided design Software for Design and the drafting micro-fluidic chip.
(2) by micro-processing technology machining needs on each layer micro-fluidic chip substrate surface and adhesive membrane micro-structural and microchannel, comprise sample holes, separate the main channel and separate subchannel.
(3) utilize double-deck adhesive membrane, with the alignment of each layer micro-fluidic chip, bonding, pressurizing and sealing, form the mobile controlled micro-fluidic chip of droplet.
(4) carry out the temperature-responsive molecule in the microchannel to the surface modification of microchannel.
(5) power-on, switching temperature between the microfluid entrance and exit, the wellability of microchannel surface is from hydrophilic to hydrophobic the conversion.
Among the present invention, can be PMMA, PC, PVC, COC, copper, aluminium, stainless steel, silicon chip, glass wafer based on the chip substrates of the temperature-responsive Micropump of micro-fluidic chip, also commercially available all kinds of common CD CD.
Among the present invention, the micro-fluidic chip that the microfluid of temperature-responsive is self-driven and the micro-structural of adhesive membrane and microchannel can be by numerical control mill quarter, laser ablation, LIGA technology, method of molding, pressure sintering, chemical attack preparations, also available soft lithographic technique preparation.
Among the present invention, the self-driven micro-fluidic chip of the microfluid of temperature-responsive is comprised of layers of chips, fits with adhesive membrane between each layer chip, and adhesive membrane can be that double-deck power causes adhesive membrane, also common double faced adhesive tape film.
Among the present invention, finishing is carried out with the temperature-responsive molecule in the microchannel of the micro-fluidic chip that the microfluid of temperature-responsive is self-driven.
Among the present invention, the temperature-responsive molecule of the micro-fluidic chip that the microfluid of temperature-responsive is self-driven produces hydrophilic and hydrophobic variation in different temperature.
Among the present invention, what the self-driven temperature control system of the microfluid of temperature-responsive adopted is low-tension supply, and scope is the 0-200 volt, and temperature is controlled at the 0-100 degree.
Self-driven micro-fluidic chip of microfluid of the temperature-responsive that the present invention proposes and preparation method thereof, from bottom to top self-driven simple to operate, as to have realized microfluid, greatly reduced the cost that microfluid drives, have portable, economic, characteristics fast and efficiently, in the association areas such as electrophoretic separation, chromatographic isolation, immunoassay, analysis, have a good application prospect.
Description of drawings
Fig. 1. the structural representation of the self-driven micro-fluidic chip of microfluid of temperature-responsive.
A. solution entrance, b. solution subentry, c. solution subentry, d. solution subentry, e. taphole, b, c, d to e are microchannel and the micro-structurals of temperature-responsive molecular modification, the flow direction of f. microfluid.
Specific embodiments
Embodiment 1
Micro-structural and the microchannel figure of the layers of chips of the micro-fluidic chip that Computer-aided design Software for Design and drafting microfluidic flow are controlled.Utilize micro-structural and the microchannel of numerical control CNC system processing preparation two-layer polymethyl methacrylate (PMMA) chip, clean each layer chip with running water, distilled water respectively, and with spots such as the residual fingerprint of ethanol chip surface, oil stains.On the double faced adhesive tape film, with required micro-structural and the microchannel of carving machine processing preparation.With layers of chips carefully align, bonding, pressurizing and sealing, make the temperature-responsive Micropump based on micro-fluidic chip.The sample introduction hand-hole that sample solution is added micro-fluidic chip, solution enters in the microchannel under the driving of external peristaltic pump, utilize the external temp control system to carry out temperature adjusting, hydrophilic and hydrophobic wellability variation occurs in the temperature-responsive molecule at the micro-structure surface of microchannel, solution is along with the variation generation self-driving type of microchannel and micro-structure surface performance flows, realized at last microfluid from bottom to top without flowing of driving.
Embodiment 2
Micro-structural and the microchannel figure of the layers of chips of Computer-aided design Software for Design and drafting centrifugal type microfludic chip.Utilize micro-structural and the microchannel of numerical control CNC system processing preparation two-layer disc-shaped Merlon (PC) chip, clean each layer chip with running water, distilled water respectively, and with spots such as the residual fingerprint of ethanol chip surface, oil stains.On the double faced adhesive tape film, with required micro-structural and the microchannel of carving machine processing preparation.With layers of chips carefully align, bonding, pressurizing and sealing, make the temperature-responsive Micropump based on micro-fluidic chip.The sample introduction hand-hole that sample solution is added micro-fluidic chip, solution enters in the microchannel under the driving of external peristaltic pump, utilize the external temp control system to carry out temperature adjusting, hydrophilic and hydrophobic wellability variation occurs in the temperature-responsive molecule at the micro-structure surface of microchannel, solution is along with the variation generation self-driving type of microchannel and micro-structure surface performance flows, realized at last microfluid from bottom to top without flowing of driving.
Claims (10)
1. self-driven micro-fluidic chip of microfluid of a temperature-responsive and preparation method thereof, there are micro-structural and microchannel in this micro-fluidic chip surface, by the temperature-responsive molecule microchannel surface is carried out modification, under the thermal stimulus of outfield, the surface topography of microchannel and the invertibity of chemical structure change the variation that causes surface wettability, the control microfluid is in the from the bottom up motion of microchannel surface, thereby the spontaneous of realization microfluid flowed.
2. press self-driven micro-fluidic chip of microfluid of temperature-responsive claimed in claim 1 and preparation method thereof, it is characterized in that, its making step is as follows:
(1) micro-structural and the microchannel figure of each layer chip in Computer-aided design Software for Design and the drafting micro-fluidic chip.
(2) by micro-processing technology machining needs on each layer micro-fluidic chip substrate surface and adhesive membrane micro-structural and microchannel, comprise sample holes, separate the main channel and separate subchannel.
(3) utilize double-deck adhesive membrane, with the alignment of each layer micro-fluidic chip, bonding, pressurizing and sealing, form the mobile controlled micro-fluidic chip of droplet.
(4) carry out the temperature-responsive molecule in the microchannel to the surface modification of microchannel.
(5) power-on, switching temperature between the microfluid entrance and exit, the wellability of microchannel surface is from hydrophilic to hydrophobic the conversion.
3. press self-driven micro-fluidic chip of microfluid of claim 1 or 2 described temperature-responsives and preparation method thereof, it is characterized in that, the Core Feature device of the self-driven micro-fluidic chip of microfluid of this temperature-responsive is micro-fluidic chip, and this chip can be produced in batches, repeatedly utilization, flexible design and assembling.
4. press self-driven micro-fluidic chip of microfluid of claim 1 or 2 described temperature-responsives and preparation method thereof, it is characterized in that, the micro-structural of the self-driven micro-fluidic chip of microfluid of this temperature-responsive and microchannel be micro-processing method by numerical control mill quarter, laser ablation, LIGA technology, method of molding, pressure sintering, chemical attack, soft lithographic technique in the preparation of chip substrates surface, size is in micron level.
5. press self-driven micro-fluidic chip of microfluid of claim 1 or 2 described temperature-responsives and preparation method thereof, it is characterized in that, the self-driven micro-fluidic chip of the microfluid of this temperature-responsive is to be formed by stacking by layers of chips, consists of micro-structural and the microchannel network of 3 D stereo.
6. press self-driven micro-fluidic chip of microfluid of claim 1 or 2 described temperature-responsives and preparation method thereof, it is characterized in that, the self-driven micro-fluidic chip of the microfluid of this temperature-responsive can be made many group micro-structurals and microchannel at chip piece, consist of multicomponent current control unit, the branch direction of alternative control microfluidic flow.
7. press self-driven micro-fluidic chip of microfluid of claim 1 or 2 described temperature-responsives and preparation method thereof, it is characterized in that, the self-driven micro-fluidic chip of the microfluid of this temperature-responsive has carried out the finishing of temperature-responsive molecule in microchannel surface.
8. press self-driven micro-fluidic chip of microfluid of claim 1 or 2 described temperature-responsives and preparation method thereof, it is characterized in that, the self-driven micro-fluidic chip of the microfluid of this temperature-responsive carries out hydrophilic and hydrophobic conversion regulation and control by applying temperature control in microchannel surface.
9. press self-driven micro-fluidic chip of microfluid of claim 1 or 2 described temperature-responsives and preparation method thereof, it is characterized in that, the self-driven micro-fluidic chip of the microfluid of this temperature-responsive can be realized the flowing from bottom to top without drive-type of microfluid.
10. press self-driven micro-fluidic chip of microfluid of claim 1 or 2 described temperature-responsives and preparation method thereof, it is characterized in that, it is portable, economical, quick, efficient that the self-driven micro-fluidic chip of the microfluid of this temperature-responsive has, and is with a wide range of applications in electrophoretic separation, chromatographic isolation, immunoassay, the related numerous association areas of reaction.
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| CN104130932A (en) * | 2014-07-30 | 2014-11-05 | 华中科技大学 | Agarose microfluidic chip based bacteria enrichment device |
| CN110054146A (en) * | 2019-03-18 | 2019-07-26 | 北京航空航天大学 | A kind of periodicity V-type micro-column structure composite membrane and the preparation method and application thereof |
| CN110573256A (en) * | 2016-12-30 | 2019-12-13 | 罗氏血液诊断股份有限公司 | sample processing system and method |
| CN115283034A (en) * | 2022-08-21 | 2022-11-04 | 东北电力大学 | Micro-fluidic chip based on light-temperature coupling response hydrogel |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104130932A (en) * | 2014-07-30 | 2014-11-05 | 华中科技大学 | Agarose microfluidic chip based bacteria enrichment device |
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| CN110054146A (en) * | 2019-03-18 | 2019-07-26 | 北京航空航天大学 | A kind of periodicity V-type micro-column structure composite membrane and the preparation method and application thereof |
| CN110054146B (en) * | 2019-03-18 | 2021-08-24 | 北京航空航天大学 | Periodic V-shaped micro-column structure composite membrane and preparation method and application thereof |
| CN115283034A (en) * | 2022-08-21 | 2022-11-04 | 东北电力大学 | Micro-fluidic chip based on light-temperature coupling response hydrogel |
| CN115283034B (en) * | 2022-08-21 | 2023-05-16 | 东北电力大学 | Micro-fluidic chip based on light-temperature coupling response hydrogel |
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Application publication date: 20130424 |