CN103041881A - PH-responded micro-fluid self-driven micro-fluidic chip and preparation method thereof - Google Patents
PH-responded micro-fluid self-driven micro-fluidic chip and preparation method thereof Download PDFInfo
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- CN103041881A CN103041881A CN2012105895790A CN201210589579A CN103041881A CN 103041881 A CN103041881 A CN 103041881A CN 2012105895790 A CN2012105895790 A CN 2012105895790A CN 201210589579 A CN201210589579 A CN 201210589579A CN 103041881 A CN103041881 A CN 103041881A
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Abstract
The invention relates to a pH-responded micro-fluid self-driven micro-fluidic chip and a preparation method thereof. A micro-structure and a micro-channel are formed in the surface of the micro-fluidic chip; the surface of the micro-channel is modified through pH-responded molecules; under different pH stimulation, the change of the surface wettability of the surface is caused by the surface appearance of the micro-channel and the reversible change of the chemical configuration; the micro-fluid is controlled to move from bottom to top on the surface of the micro-channel so as to realize self-flowing of the micro-fluid; and the micro-fluidic chip is mainly applied to relevant fields of electrophoretic separation, chromatographic separation, immunoassay, reaction and the like. The micro-fluidic chip is simple to operate, realizes self-driving of the micro-fluid from bottom to top, greatly reduces the cost in driving the micro-fluid, has the characteristics of being portable, economic, rapid and efficient, and provides a completely new micro-fluid flowing control technique for driving-less flowing of the micro-fluid.
Description
Technical field
The present invention relates to self-driven micro-fluidic chip of microfluid of a kind of pH response and preparation method thereof, there are micro-structural and microchannel in this micro-fluidic chip surface, by pH response molecule microchannel surface is carried out modification, under different pH stimulate, the surface topography of microchannel and the invertibity of chemical structure change the variation that causes surface wettability, the control microfluid is in the from the bottom up motion of microchannel surface, thereby realize the spontaneous mobile of microfluid, be mainly used in the association areas such as electrophoretic separation, chromatographic isolation, immunoassay, reaction.
Background technology
Microfluidic analysis chip has microminiaturization, automation, the advantage such as integrated, convenient and quick as a kind of novel analysis platform; obtain a wide range of applications for example synthetic screening of cell biology, analytical chemistry, environmental monitoring and protection, judicial expertise and medicine in a lot of fields.In microfluidic analysis chip, the accurate sample introduction of micro liquid is the key of sample treatment and analysis, just need such operation in for example micro-fluid control chip electrophoretic separation, chromatographic isolation, the immunoassay, this is because the characteristics of microfluidic analysis chip will operate the microfluid under the micro-scale and control, and it is extremely small as the Fluid Volume of operation and control object, cause flow behavior and the macroscopic view of microfluid to be very different, negligible phenomenon becomes the major influence factors of Fluid Flow in A under micro-scale under macro-scale.In recent years, on microfluidic analysis chip, how to realize driving and control to microfluid, become a research difficult problem and focus in the microfluidic analysis chip technology.
Driving to microfluid in the micro-fluidic chip can realize by Micropump.In recent years, along with development and the maturation of microflow control technique, research and develop a lot of methods and device, realized to a certain extent the driving to microfluid in the micro-fluidic chip, also had some limitation simultaneously.Aspect Micropump research, comprise mechanical Micropump and on-mechanical Micropump.For example, compression pump (machinery or syringe pump) simple to operate, easy realization, with low cost, but be difficult for realizing miniaturization at chip.Hot gas power Micropump braking mechanism is simple, conditional request is low, be fit to batch production, but because heat lag, braking frequency is lower.The characteristics of mems electrostatic pump are that braking frequency is high, simple in structure, but brake force is lower.The electromagnetism Micropump has larger brake force, and operating voltage is low, but does not have advantage on the volume.The electrohydrodynamic micro-pump structure is simple, easily processing, cost are low, but requires harsh to the dielectric property of liquid stream.Driven by electroosmosis is to use the widest microfluid to drive and control technology in the present microfluidic analysis chip, have flow velocity steadily, no pulse, can realize that the liquid stream of valveless switches, but require material surface energy that electric charge is provided, high voltage source is difficult for microminiaturized, and has heating and air bubble problem.At present, the driving of microfluid and the research of control are then focused mostly in decorative layer molecular configuration physical responses, for example gelling temp or pH response, but the original position under micro-scale preparation, preparation process is complicated, condition is harsh etc. has limited their application.Therefore, driving microfluid in the micro-fluidic chip by the infiltrating variation of control surface, should be one of research direction of little valve/Micropump on the micro-fluidic chip.
Yet, routine techniques is difficult to finish the intelligent drives to microfluid in the microchannel, therefore, thereby the variation by regulation and control pH comes the infiltrating variation of control surface to drive microfluid in the micro-fluidic chip, develop a kind of convenient, quick, efficiently, microfluid actuation techniques cheaply, should be how to control one of research direction of microfluid on the micro-fluidic chip, substantial breakthrough is not yet arranged at present.
Summary of the invention
The purpose of this invention is to provide self-driven micro-fluidic chip of microfluid of a kind of pH response and preparation method thereof, there are micro-structural and microchannel in this micro-fluidic chip surface, by pH response molecule microchannel surface is carried out modification, under different pH stimulate, the surface topography of microchannel and the invertibity of chemical structure change the variation that causes surface wettability, the control microfluid is in the from the bottom up motion of microchannel surface, thereby realize the spontaneous mobile of microfluid, be mainly used in the association areas such as electrophoretic separation, chromatographic isolation, immunoassay, reaction.
For achieving the above object, the present invention adopts following operating procedure:
(1) micro-structural and the microchannel figure of each layer chip in Computer-aided design Software for Design and the drafting micro-fluidic chip.
(2) by micro-processing technology machining needs on each layer micro-fluidic chip substrate surface and adhesive membrane micro-structural and microchannel, comprise sample holes, separate the main channel and separate subchannel.
(3) utilize double-deck adhesive membrane, with the alignment of each layer micro-fluidic chip, bonding, pressurizing and sealing, form the mobile controlled micro-fluidic chip of droplet.
(4) carry out pH response molecule in the microchannel to the surface modification of microchannel.
(5) the pH value of switching solution in microfluidic channel, the wellability of microchannel surface is from hydrophilic to hydrophobic the conversion.
Among the present invention, the chip substrates of the self-driven micro-fluidic chip of microfluid of pH response can be PMMA, PC, PVC, COC, copper, aluminium, stainless steel, silicon chip, glass wafer, also commercially available all kinds of common CD CD.
Among the present invention, the chip of the self-driven micro-fluidic chip of microfluid of pH response and the micro-structural of adhesive membrane and microchannel can be by numerical control mill quarter, laser ablation, LIGA technology, method of molding, pressure sintering, chemical attack preparation, also available soft lithographic technique preparations.
Among the present invention, the self-driven micro-fluidic chip of microfluid of pH response is comprised of layers of chips, fits with adhesive membrane between each layer chip, and adhesive membrane can be that double-deck power causes adhesive membrane, also common double faced adhesive tape film.
Among the present invention, finishing is carried out with pH response molecule in the microchannel of the self-driven micro-fluidic chip of microfluid of pH response.
Among the present invention, the pH response molecule of the self-driven micro-fluidic chip of microfluid of pH response produces hydrophilic and hydrophobic variation at different pH.
Self-driven micro-fluidic chip of microfluid of the pH response that the present invention proposes and preparation method thereof, from bottom to top self-driven simple to operate, as to have realized microfluid, greatly reduced the cost that microfluid drives, have portable, economic, characteristics fast and efficiently, in the association areas such as electrophoretic separation, chromatographic isolation, immunoassay, reaction, have a good application prospect.
Description of drawings
The structural representation of the self-driven micro-fluidic chip of microfluid of Fig. 1 .pH response.
A. solution entrance, b. solution subentry, c. solution subentry, d. solution subentry, e. taphole, b, c, d to e are microchannel and the micro-structurals of pH response molecular modification, the flow direction of f. microfluid.
Specific embodiments
Embodiment 1
Micro-structural and the microchannel figure of the layers of chips of the micro-fluidic chip that Computer-aided design Software for Design and drafting microfluidic flow are controlled.Utilize micro-structural and the microchannel of numerical control CNC system processing preparation two-layer polymethyl methacrylate (PMMA) chip, clean each layer chip with running water, distilled water respectively, and with spots such as the residual fingerprint of ethanol chip surface, oil stains.On the double faced adhesive tape film, with required micro-structural and the microchannel of carving machine processing preparation.With layers of chips carefully align, bonding, pressurizing and sealing, make the intelligent Micropump based on micro-fluidic chip.The sample introduction hand-hole that sample solution is added micro-fluidic chip, solution enters in the microchannel under the driving of external peristaltic pump, Micropump zone in the microchannel, the surface responds the pH value of solution, obtain hydrophilic and hydrophobic switching, solution is along with the variation generation self-driving type of microchannel and micro-structure surface performance flows, realized at last microfluid from bottom to top without flowing of driving.
Embodiment 2
Micro-structural and the microchannel figure of the layers of chips of Computer-aided design Software for Design and drafting centrifugal type microfludic chip.Utilize micro-structural and the microchannel of numerical control CNC system processing preparation two-layer disc-shaped Merlon (PC) chip, clean each layer chip with running water, distilled water respectively, and with spots such as the residual fingerprint of ethanol chip surface, oil stains.On the double faced adhesive tape film, with required micro-structural and the microchannel of carving machine processing preparation.With layers of chips carefully align, bonding, pressurizing and sealing, make the intelligent Micropump based on micro-fluidic chip.The sample introduction hand-hole that sample solution is added micro-fluidic chip, solution enters in the microchannel under the driving of external peristaltic pump, Micropump zone in the microchannel, the surface responds the pH value of solution, obtain hydrophilic and hydrophobic switching, solution is along with the variation generation self-driving type of microchannel and micro-structure surface performance flows, realized at last microfluid from bottom to top without flowing of driving.
Claims (10)
1. self-driven micro-fluidic chip of microfluid of pH response and preparation method thereof, there are micro-structural and microchannel in this micro-fluidic chip surface, by pH response molecule microchannel surface is carried out modification, under different pH stimulate, the surface topography of microchannel and the invertibity of chemical structure change the variation that causes surface wettability, the control microfluid is in the from the bottom up motion of microchannel surface, thereby the spontaneous of realization microfluid flowed.
2. self-driven micro-fluidic chip of microfluid that responds by pH claimed in claim 1 and preparation method thereof is characterized in that its making step is as follows:
(1) micro-structural and the microchannel figure of each layer chip in Computer-aided design Software for Design and the drafting micro-fluidic chip.
(2) by micro-processing technology machining needs on each layer micro-fluidic chip substrate surface and adhesive membrane micro-structural and microchannel, comprise sample holes, separate the main channel and separate subchannel.
(3) utilize double-deck adhesive membrane, with the alignment of each layer micro-fluidic chip, bonding, pressurizing and sealing, form the mobile controlled micro-fluidic chip of droplet.
(4) carry out pH response molecule in the microchannel to the surface modification of microchannel.
(5) the pH value of switching solution in microfluidic channel, the wellability of microchannel surface is from hydrophilic to hydrophobic the conversion.
3. self-driven micro-fluidic chip of microfluid that responds by claim 1 or 2 described pH and preparation method thereof, it is characterized in that, the Core Feature device of the self-driven micro-fluidic chip of microfluid of this pH response is micro-fluidic chip, and this chip can be produced in batches, repeatedly utilization, flexible design and assembling.
4. self-driven micro-fluidic chip of microfluid that responds by claim 1 or 2 described pH and preparation method thereof, it is characterized in that, the micro-structural of the self-driven micro-fluidic chip of microfluid of this pH response and microchannel be micro-processing method by numerical control mill quarter, laser ablation, LIGA technology, method of molding, pressure sintering, chemical attack, soft lithographic technique in the preparation of chip substrates surface, size is in micron level.
5. self-driven micro-fluidic chip of microfluid that responds by claim 1 or 2 described pH and preparation method thereof, it is characterized in that, the self-driven micro-fluidic chip of microfluid of this pH response is to be formed by stacking by layers of chips, consists of micro-structural and the microchannel network of 3 D stereo.
6. self-driven micro-fluidic chip of microfluid that responds by claim 1 or 2 described pH and preparation method thereof, it is characterized in that, the self-driven micro-fluidic chip of microfluid of this pH response can be made many group micro-structurals and microchannel at chip piece, consist of many group control modules, flow direction and the multichannel flow branch of alternative control microfluid.
7. self-driven micro-fluidic chip of microfluid that responds by claim 1 or 2 described pH and preparation method thereof is characterized in that the self-driven micro-fluidic chip of microfluid of this pH response has carried out the finishing of pH response molecule in microchannel surface.
8. self-driven micro-fluidic chip of microfluid that responds by claim 1 or 2 described pH and preparation method thereof, it is characterized in that, the self-driven micro-fluidic chip of microfluid of this pH response carries out hydrophilic and hydrophobic conversion by the automatic identification of pH response molecule in microchannel surface.
9. press self-driven micro-fluidic chip of microfluid of claim 1 or 2 described pH responses and preparation method thereof, it is characterized in that, the self-driven micro-fluidic chip of microfluid of this pH response can realize fast that microfluid nothing from bottom to top drives mobile.
10. self-driven micro-fluidic chip of microfluid that responds by claim 1 or 2 described pH and preparation method thereof, it is characterized in that, the self-driven micro-fluidic chip of microfluid of this pH response has portable, economical, quick, efficient, is with a wide range of applications in electrophoretic separation, chromatographic isolation, immunoassay, the related numerous association areas of reaction.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110013891A (en) * | 2019-01-24 | 2019-07-16 | 厦门大学 | A kind of microfluidic system and control method based on bionical responsiveness liquid gate |
| CN110433881A (en) * | 2019-09-02 | 2019-11-12 | 丹娜（天津）生物科技有限公司 | A kind of hydrophilic modification method of micro-fluidic chip microchannel material |
| CN111721818A (en) * | 2020-06-04 | 2020-09-29 | 南京市食品药品监督检验院 | Self-driven electrochemical detection chip and preparation method thereof |
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| CN101472940A (en) * | 2006-04-18 | 2009-07-01 | 先进液体逻辑公司 | Droplet-based biochemistry |
| CN102059161A (en) * | 2009-11-18 | 2011-05-18 | 中国科学院化学研究所 | Microfluidic chip and manufacturing method thereof |
| CN102784671A (en) * | 2012-08-13 | 2012-11-21 | 苏州汶颢芯片科技有限公司 | Centrifugal micro-fluidic chip for detecting pesticide residue and preparation method thereof |
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- 2012-12-31 CN CN2012105895790A patent/CN103041881A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101472940A (en) * | 2006-04-18 | 2009-07-01 | 先进液体逻辑公司 | Droplet-based biochemistry |
| CN102059161A (en) * | 2009-11-18 | 2011-05-18 | 中国科学院化学研究所 | Microfluidic chip and manufacturing method thereof |
| CN102784671A (en) * | 2012-08-13 | 2012-11-21 | 苏州汶颢芯片科技有限公司 | Centrifugal micro-fluidic chip for detecting pesticide residue and preparation method thereof |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110013891A (en) * | 2019-01-24 | 2019-07-16 | 厦门大学 | A kind of microfluidic system and control method based on bionical responsiveness liquid gate |
| CN110013891B (en) * | 2019-01-24 | 2020-09-25 | 厦门大学 | Microfluidic system based on bionic responsive liquid gating and control method |
| CN110433881A (en) * | 2019-09-02 | 2019-11-12 | 丹娜（天津）生物科技有限公司 | A kind of hydrophilic modification method of micro-fluidic chip microchannel material |
| CN111721818A (en) * | 2020-06-04 | 2020-09-29 | 南京市食品药品监督检验院 | Self-driven electrochemical detection chip and preparation method thereof |
| CN111721818B (en) * | 2020-06-04 | 2022-07-05 | 南京市食品药品监督检验院 | Self-driven electrochemical detection chip and preparation method thereof |
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Application publication date: 20130417 |