CN106076446A - A kind of pair of branch road realizes the microchannel of interval microlayer model fusion function - Google Patents
A kind of pair of branch road realizes the microchannel of interval microlayer model fusion function Download PDFInfo
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- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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Abstract
A kind of pair of branch road realizes the microchannel of interval microlayer model fusion function, and this microchannel comprises two functional units, and one is microlayer model signal generating unit, and this microlayer model signal generating unit is to generate the T-shaped passage of microlayer model；Another is to realize microlayer model integrated unit, and two bypass passages that this microlayer model integrated unit is converged again by bifurcated downstream, upstream are constituted.Microlayer model signal generating unit is connected with oil phase entrance with aqueous phase entrance.Double branch structures that the bifurcated downstream, upstream that microlayer model integrated unit is made up of the first branch road and the second branch road is converged again form, first branch road and the second branch road two connect composition square shape structure, first branch road is inverted U-shaped, second branch road is the horizontal structure of bottom, and the end of the second branch road is provided with spherical port.Present configuration is simple and can realize the controlled fusion function of drop flexibly, and this will occur complicated controlled biochemical reaction to provide safeguard for microlayer model.
Description
Technical field
Picoliters is to the microlayer model of nanoliter volumes scope because having the advantages such as no cross contamination, and it is at blood testing, protein
The biochemistry such as crystallization, cell analysis, polymerase chain reaction (PCR), medical diagnosis on disease, granule synthesis present huge application and dive
Power.
Background technology
Microfluidic chip technology (Microfluidics) is otherwise known as chip lab (Lab-on-a-chip), can be one
The basic function of integrated traditional biological and chemical laboratory on the micro chip of individual several square centimeters, separates including sample, makes
The operations such as standby, chemical reaction, detection.Drop microflow control technique is an important branch of microfluidic chip technology.Microlayer model because of
Having flux high, the advantage such as no cross contamination, it is at inkjet printing, microring array, DNA analysis, materials synthesis, crystallization of protein etc.
Field presents huge application potential.Some biochemical reactions based on microlayer model, as micro-nano particle synthesize, during need by
Two kinds of different droplet coalescence are together, in order to obtain preferable hybrid reaction effect.Thus, the controlled fusion of drop becomes
One important drop microflow control technique, particularly significant for realizing multistep reaction in drop.
Summary of the invention
A kind of pair of branch road realizes the microchannel of interval microlayer model fusion function, and the present invention is to converge again based on bifurcated downstream, upstream
The double branch road microchannels flow resistance difference principle closed, devises and realizes the microchannel that interval microlayer model blends.
A kind of pair of branch road realizes the microchannel of interval microlayer model fusion function, and this microchannel comprises two functional units, and one
Being microlayer model signal generating unit 9, this microlayer model signal generating unit 9 is the T-shaped passage in order to generate microlayer model；Another is to realize microlayer model
Integrated unit 10, two bypass passages that this microlayer model integrated unit 10 is converged again by bifurcated downstream, upstream are constituted.Microlayer model generates
Unit 9 is connected with oil phase entrance 7 with aqueous phase entrance 8.Microlayer model integrated unit 10 is by the first branch road 11 and the second branch road 12
Double branch structures composition that the bifurcated downstream, upstream constituted is converged again, the first branch road 11 and the second branch road 12 liang connect composition mouth word
Shape structure, the first branch road 11 is inverted U-shaped, and the second branch road 12 is the horizontal structure of bottom, and the end of the second branch road 12 is provided with
Spherical port.
During work, discrete phase (aqueous phase) is connected with aqueous phase entrance 8 by syringe pump, continuous phase (oil phase) by syringe pump and
Oil phase entrance 7 is connected, and outlet 13 is connected to liquid pool by PE pipe.It is applied to the shearing force of discrete phase and pressure is common in continuous phase
Under effect, generating uniform microlayer model at drop formation unit 9, this does " early-stage preparations " for the follow-up fusion of drop.This
After, there are different fusion behaviors from the drop in the second branch road 12 in the first branch road 11.
First drop 1 moves in the first branch road 11, flow resistance R of the first branch road 1111Flow resistance less than the second branch road 12
R12；The drop of motion adds flow resistance R of this branch road11, and increased to over flow resistance R of the second branch road 1212；Second drop 2 is then
The second branch road 12 is selected to move.Owing to the first drop 1 makes first path along " necking down " structure of the first spherical port of branch road 11
Flow resistance R of 1111Become big, so that the 3rd drop 3 and the 4th drop 4 continue to move to the second branch road 12.Now in the second branch road 12
Second drop the 2, the 3rd drop the 3, the 4th drop 4 considerably increases R12, so that the 5th drop 5 moves to the first branch road 11 and second
During the upstream junction of branch road 12, the downstream that the first drop 1 " is extruded " to the first branch road 11 and the second branch road 12 by continuous phase is handed over
Merge with the second drop 2 at remittance.In order to make drop be faster than the first branch road 11 in the movement velocity of the second branch road 12, and by
The width of two branch roads 12 reduces, with the purpose reaching the first branch road drop 1 and the second branch road drop 2 merges in downstream intersection.
After occurring to merge for the first time, the fully out first branch road branch road 11 of the first drop 1, by the 5th follow-up drop 5 " supplementing " to the
One branch road 11.5th drop 5 and the 3rd drop 3 carry out second time and merge, and the 6th drop 6 and the 4th drop 4 carry out third time and melt
Close.Hereafter, so repeating, it is adjacent liquid the most for the first time that the second branch road 12 occurs three times to merge with the drop of the first branch road 11
Dripping and merge, second time and third time are one droplet coalescence in interval.Therefore, the microchannel of this invention not only can realize adjacent drops
Merging but also can realize being spaced the function of droplet coalescence, the biochemical reaction that complexity occurs for microlayer model is provided the most just by this
The condition of profit.
Microchannel is formed by soft lithographic processes, and used material is polydimethylsiloxane (PDMS).
The present invention, on the basis of not increasing extra driving or controlling device, utilizes simple two branch roads to cross structure not only
Adjacent drops can be realized merge but also the function that can realize being spaced droplet coalescence, with the existing droplet coalescence function of realizing
Microchannel is compared, and this inventive structure is simple and can realize the controlled fusion function of drop flexibly, and this will occur complicated for microlayer model
Controlled biochemical reaction provide safeguard.
Accompanying drawing explanation
Fig. 1 realizes double branch road MCA schematic diagrams that microlayer model merges
The double branch road partial structurtes enlarged diagram of Fig. 2
Fig. 3 realizes double branch road microchannel real work schematic diagrams that microlayer model merges
Detailed description of the invention
In conjunction with accompanying drawing 3, microchannel work process and the effect of the present invention are further elaborated and verify.
Microchannel real work mainly comprises aqueous phase entrance, oil phase entrance, drop formation unit, integrated unit and outlet.
During work, syringe pump is connected with oil phase entrance with aqueous phase entrance by PE pipe respectively.Outlet is connected to waste liquid pool by PE pipe.
In order to prove that this invention can realize being spaced the function of droplet coalescence, Fig. 3 gives experiment effect figure, figure (a) and figure (b) respectively
It it is experiment actual effect figure before and after two droplet coalescence.From the figure 3, it may be seen that this invention can reach intended syncretizing effect.
Claims (3)
1. double branch roads realize being spaced the microchannel of microlayer model fusion function, it is characterised in that: this microchannel comprises two merits
Energy unit, one is microlayer model signal generating unit (9), and this microlayer model signal generating unit (9) is in order to the T-shaped passage generating microlayer model；Separately
One is to realize microlayer model integrated unit (10), and two branch roads that this microlayer model integrated unit (10) is converged again by bifurcated downstream, upstream lead to
Road is constituted；Microlayer model signal generating unit (9) is connected with oil phase entrance (7) with aqueous phase entrance (8)；Microlayer model integrated unit (10) is
Double branch structures that the bifurcated downstream, upstream being made up of the first branch road (11) and the second branch road (12) is converged again form, the first branch road
(11) and the second branch road (12) two connects composition square shape structure, and the first branch road (11) is inverted U-shaped, and the second branch road (12) is
The horizontal structure of bottom, the end of the second branch road (12) is provided with spherical port；
During work, discrete phase is connected with aqueous phase entrance (8) by syringe pump, and continuous phase passes through syringe pump and oil phase entrance (7) phase
Even, outlet (13) is connected to liquid pool by PE pipe；Under continuous phase is applied to the shearing force of discrete phase and pressure acts on jointly,
Drop formation unit (9) place generates uniform microlayer model, and this does " early-stage preparations " for the follow-up fusion of drop；Hereafter, first
There are different fusion behaviors in branch road (11) from the drop in the second branch road (12).
A kind of pair of branch road the most according to claim 1 realizes the microchannel of interval microlayer model fusion function, it is characterised in that:
First drop (1) moves in the first branch road (11), flow resistance R of the first branch road (11)11Flow resistance less than the second branch road (12)
R12；The drop of motion adds flow resistance R of this branch road11, and increased to over flow resistance R of the second branch road (12)12；Second drop
(2) the second branch road (12) is then selected to move；Owing to the first drop (1) makes along " necking down " structure of the first branch road (11) spherical port
Obtain flow resistance R of first path (11)11Become big, so that the 3rd drop (3) and the 4th drop (4) continue to move to the second branch road
(12)；Now in the second branch road (12), the second drop (2), the 3rd drop (3), the 4th drop (4) considerably increase R12, so that the
When five drops (5) move to the upstream junction of the first branch road (11) and the second branch road (12), the first drop (1) is by continuous phase
The downstream intersection " extruded " to the first branch road (11) and the second branch road (12) merges with the second drop (2)；In order to make liquid
The movement velocity dropping in the second branch road (12) is faster than the first branch road (11), and is reduced by the width of the second branch road (12), to reach
The purpose that first branch road drop (1) and the second branch road drop (2) merge in downstream intersection；After occurring to merge for the first time, first
The fully out first branch road branch road (11) of drop (1), by the 5th follow-up drop (5) " supplementing " to the first branch road (11)；5th
Drop (5) and the 3rd drop (3) carry out second time and merge, and the 6th drop (6) and the 4th drop (4) carry out third time and merge；This
After, so repeating, it is adjacent liquid the most for the first time that the second branch road (12) occurs three times to merge with the drop of the first branch road (11)
Dripping and merge, second time and third time are one droplet coalescence in interval；Therefore, the microchannel of this invention not only can realize adjacent drops
Merging but also can realize being spaced the function of droplet coalescence, the biochemical reaction that complexity occurs for microlayer model is provided the most just by this
The condition of profit.
A kind of pair of branch road the most according to claim 1 realizes the microchannel of interval microlayer model fusion function, it is characterised in that:
Microchannel is formed by soft lithographic processes, and used material is polydimethylsiloxane.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108273454A (en) * | 2016-12-27 | 2018-07-13 | 中国科学院微生物研究所 | A kind of method that nanoliter level microlayer model merges in small-sized reaction tube |
| CN109647547A (en) * | 2017-10-12 | 2019-04-19 | 中国科学院大连化学物理研究所 | A kind of preparation method of the controllable aqueous two-phase drop based on microflow control technique |
| CN110575851A (en) * | 2018-06-07 | 2019-12-17 | 洛阳华清天木生物科技有限公司 | Device and chip for micro-droplet quantitative segmentation and fusion and micro-droplet quantitative segmentation and fusion method |
| CN111068799A (en) * | 2018-10-18 | 2020-04-28 | 浙江达普生物科技有限公司 | Microfluidic channel for generating droplets and use thereof |
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| CN106076446B (en) | 2018-12-07 |
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