CN103386333A - Micro-fluidic liquid drop production chip - Google Patents

Micro-fluidic liquid drop production chip Download PDF

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Publication number
CN103386333A
CN103386333A CN2013103415401A CN201310341540A CN103386333A CN 103386333 A CN103386333 A CN 103386333A CN 2013103415401 A CN2013103415401 A CN 2013103415401A CN 201310341540 A CN201310341540 A CN 201310341540A CN 103386333 A CN103386333 A CN 103386333A
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China
Prior art keywords
drop
micro
fluidic
chip
passage
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CN2013103415401A
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Chinese (zh)
Inventor
叶嘉明
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SUZHOU YOUNGCHIP CHIP TECHNOLOGY Co Ltd
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SUZHOU YOUNGCHIP CHIP TECHNOLOGY Co Ltd
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Priority to CN2013103415401A priority Critical patent/CN103386333A/en
Publication of CN103386333A publication Critical patent/CN103386333A/en
Pending legal-status Critical Current

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Abstract

In order to meet requirements for bulk production of liquid drops, the invention designs a novel micro-fluidic liquid drop production chip. The novel micro-fluidic liquid drop production chip is characterized by consisting of an oil phase inlet, an oil phase introduction passage, a water phase inlet, a water phase introduction passage, a water phase storage chamber, a liquid drop generation chamber, a liquid drop passage and an outlet. An external pump valve system is used for respectively injecting oil phase and water phase solutions from respective inlets, the water in the water phase storage chamber passes through an array through hole structure of an intermediate layer and then a corresponding number of micro liquid drops are generated in the liquid drop generation chamber, and then are carried into the liquid drop passage by an oil phase, guided out of the outlet and collected. The micro-fluidic liquid drop production chip provided by the invention can be used for generating a large amount of micro liquid drops online in real time, so that the production efficiency of liquid drops is significantly improved.

Description

A kind of micro-fluidic drop is produced chip
Technical field
The present invention relates to the micro-fluidic chip field, particularly relate to a kind of micro-fluidic drop and produce chip.
Background technology
In prior art, micro-fluidic chip is an emerging technology platform.On the chip of more than square centimeters, control fluid by the microchannel of networking, complete the various operations of conventional chemical or biology laboratory.Little drop (droplet) is a kind of technology of controlling micro volume liquid that grows up on micro-fluidic chip in recent years, its principle is: with two kinds of immiscible liquid, a kind of as continuous phase take wherein, another kind is decentralized photo, after continuous phase and decentralized photo enter microchannel simultaneously, under the effect of microchannel, decentralized photo is with micro volume (10 -15~10 -9L) formal distribution of unit in continuous phase, forms little drop of series of discrete, and each drop, as a microreactor, is completed one group of chemistry or biological respinse.Little drop is used for screening and has following advantage: 1) sample consumption is atomic, greatly reduces the screening cost; 2) drop is wrapped up by oil, exchanges the stable reaction conditions in drop, reliable results without material with the external world.Therefore the aspect such as, enzyme reaction analyzing and testing synthetic in drug screening, micro Nano material has important and application prospect widely.
At present, the generation technique of drop mainly is based on the design of chip microchannel and realizes, namely utilizes MCA T-shaped or the flow focusing type, produces continuously little drop; In addition, the pneumatic operated valve technology also is applied to the drop formation of different component.But, existing drop formation method still is difficult to realize realize generating simultaneously large batch of little drop in a micro-fluidic chip, therefore during the occasion of or screening synthetic at the drop that drop is applied to larger flux, be difficult to meet the demand of high efficiency batch production drop.
Summary of the invention
In order to meet the demand of drop batch production, the present invention has designed a kind of novel micro-fluidic drop and has produced chip, it is characterized in that this micro-fluidic chip is by oil phase entrance, oil phase introducing passage, water entrance, water introducing passage, water storage chamber, drop formation chamber, drop passage and export mixes.
Micro-fluidic drop provided by the present invention is produced chip, it is characterized in that, described micro-fluidic chip is to be prepared from by one or more in the materials such as glass, dimethyl silicone polymer, polymethyl methacrylate, Merlon, polystyrene.
Micro-fluidic drop provided by the present invention is produced chip, it is characterized in that, described micro-fluidic chip consists of cover plate layer, fluid channel layer 1, intermediate layer, fluid channel layer 2 and basalis, forms the channel network structure of a three-dimensional.
Be processed with oil phase in described micro-fluidic chip on fluid channel layer 1 and introduce passage, drop formation chamber, drop passage, the three is connected; Be processed with water on fluid channel layer 2 and introduce passage and water storage chamber, both are connected; The water storage chamber be positioned at the drop formation chamber under, separated by an intermediate layer with array through-hole between the two.
Micro-fluidic drop provided by the present invention is produced chip, it is characterized in that, the operation principle that described micro-fluidic drop is produced chip is: use the external pump valve system respectively the profit two phase liquid to be injected from entrance separately, water in the water storage chamber is after the array through-hole structure in intermediate layer, to produce little drop of respective amount in the drop formation chamber, and by oil phase, brought into the drop passage, derive and collect from outlet.
Micro-fluidic drop provided by the present invention is produced chip, it is characterized in that, uses micro-fluidic drop provided by the invention to produce chip, owing to generating a large amount of little drops in real-time online ground, thereby significantly improves drop production efficiency.
Micro-fluidic drop provided by the present invention is produced chip,, except the water-in type drop that produces oil, also can be used for a large amount of production oil-in-water type drops, and the import that only need to change simply oil phase and water gets final product.
Description of drawings
Fig. 1. a kind of micro-fluidic drop provided by the present invention is produced the structural representation of chip.Wherein, A is the cover plate layer, and B is fluid channel layer 1, and C is intermediate layer, and D is fluid channel layer 2, and E is basalis; A is that oil phase entrance, b are the water entrance, and c is that oil phase is introduced passage, and d is that water is introduced passage, and e is the water storage chamber, and f is the drop formation chamber, and g is the drop passage, and h is outlet, and i is the array micropore.
Fig. 2. a kind of micro-fluidic drop provided by the present invention is produced the cross section structure of chip and the schematic diagram of drop formation.
Specific embodiments
The following examples are further described the present invention in connection with Figure of description.
The micro-fluidic chip of 1 one kinds of Mass production drops of embodiment
The invention provides a kind of micro-fluidic drop and produce chip, this chip uses laser engraving to be prepared in conjunction with thermocompression bonding method by the PMMA substrate, and its structural representation as shown in Figure 1.Wherein, be processed with oil phase import a, water import b and drop outlet h on cover plate layer A; Be processed with oil phase on fluid channel layer B and introduce passage c, drop formation chamber f and drop passage g; Be processed with the through-hole structure i of array on the C of intermediate layer; Be processed with water on fluid channel layer D and introduce passage d and water storage chamber e; Basalis E is blank substrate.
In above-mentioned micro-fluidic chip, the operating process of Mass production drop is as shown in Figure 2 in illustrating.Use syringe pump to inject organic phase in the oil phase passage, such as mineral oil, hexadecane etc.; Use simultaneously syringe pump toward injecting the aqueous solution of pure water or sample in the water passage, this flow of solution is introduced passage through water and is entered the water storage chamber of an end sealing, drop formation chamber from the through-hole structure of array toward upper strata operation finally, under the effect of oil phase, little drop of a plurality of water-in-oil types of disposable formation, the quantity of drop depends on the quantity of micro through hole; The little drop that generates finally flows out from drop passage, outlet, can use conduit or beaker etc. to carry out the collection of drop.
Use micro-fluidic drop provided by the invention to produce chip, owing to generating a large amount of little drops in real-time online ground, thereby significantly improve drop production efficiency.Micro-fluidic drop provided by the present invention is produced chip,, except the water-in type drop that produces oil, also can be used for a large amount of production oil-in-water type drops, need to change oil phase simply and water gets final product.

Claims (6)

1. a micro-fluidic drop is produced chip, it is characterized in that this micro-fluidic chip is by oil phase entrance, oil phase introducing passage, water entrance, water introducing passage, water storage chamber, drop formation chamber, drop passage and export mixes.
2. produce chip according to a kind of micro-fluidic drop claimed in claim 1, it is characterized in that, described micro-fluidic chip is to be prepared from by one or more in the materials such as glass, dimethyl silicone polymer, polymethyl methacrylate, Merlon, polystyrene.
3. produce chip according to a kind of micro-fluidic drop claimed in claim 1, it is characterized in that, described micro-fluidic chip consists of cover plate layer, fluid channel layer 1, intermediate layer, fluid channel layer 2 and basalis, forms the channel network structure of a three-dimensional.
4. produce chip according to claim 1 and 3 described a kind of micro-fluidic drops, it is characterized in that, be processed with oil phase in described micro-fluidic chip on fluid channel layer 1 and introduce passage, drop formation chamber, drop passage, the three is connected; Be processed with water on fluid channel layer 2 and introduce passage and water storage chamber, both are connected; The water storage chamber be positioned at the drop formation chamber under, separated by an intermediate layer with array through-hole between the two.
5. produce chip according to a kind of micro-fluidic drop claimed in claim 1, it is characterized in that, the operation principle that described micro-fluidic drop is produced chip is: use the external pump valve system respectively the profit two phase liquid to be injected from entrance separately, water in the water storage chamber is after the array through-hole structure in intermediate layer, to produce little drop of respective amount in the drop formation chamber, and by oil phase, brought into the drop passage, derive and collect from outlet.
6. produce chip according to a kind of micro-fluidic drop claimed in claim 1, it is characterized in that, use micro-fluidic drop provided by the invention to produce chip, owing to generating a large amount of little drops in real-time online ground, thereby significantly improve drop production efficiency.
CN2013103415401A 2013-08-07 2013-08-07 Micro-fluidic liquid drop production chip Pending CN103386333A (en)

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Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104148124A (en) * 2014-07-30 2014-11-19 河北工业大学 Droplet formation device for micro-fluidic chips
CN105013547A (en) * 2015-07-30 2015-11-04 天津大学 Novel microbubble/liquid drop generation regulation and control device and novel microbubble/liquid drop generation regulation and control method
CN105233893A (en) * 2015-11-02 2016-01-13 华东理工大学 Method for preparing micro-droplets based on micro-fluidic chip modification technology
CN105498875A (en) * 2016-01-27 2016-04-20 杭州霆科生物科技有限公司 Centrifugal micro-fluidic chip for preparing liquid drops
CN105524829A (en) * 2015-11-25 2016-04-27 成都赫尔墨斯科技有限公司 Micro-fluidic chip for manufacturing tissue engineering micromodule
CN105618167A (en) * 2016-01-27 2016-06-01 杭州霆科生物科技有限公司 Centrifugal microfluidic chip for preparing droplets in high-throughput manner
CN106076446A (en) * 2016-08-19 2016-11-09 北京工业大学 A kind of pair of branch road realizes the microchannel of interval microlayer model fusion function
CN106110340A (en) * 2016-07-12 2016-11-16 陈传品 A kind of method and device preparing target drug-carrying microbubble
CN105013544B (en) * 2014-04-24 2016-11-23 中国科学院青岛生物能源与过程研究所 A kind of microlayer model fusion method based on the induction of hydrophilic fibers silk
CN106378217A (en) * 2016-12-06 2017-02-08 太原理工大学 Micro-fluidic chip capable of producing two kinds of droplets including oil-water-oil droplets and water-oil-water droplets
CN107583693A (en) * 2017-08-30 2018-01-16 武汉科技大学 A kind of T-shaped microchannel integrates droplet generation chip
CN108043483A (en) * 2018-02-05 2018-05-18 东南大学 High-throughput drop formation and collection device based on density adjusting
CN109294866A (en) * 2017-07-24 2019-02-01 广州康昕瑞基因健康科技有限公司 Emulsion chip and emulsion preparing device
CN111013676A (en) * 2019-12-17 2020-04-17 江苏圣极基因科技有限公司 Liquid drop preparation method and micro-fluidic chip
WO2020082487A1 (en) * 2018-10-23 2020-04-30 深圳市博瑞生物科技有限公司 Microfluidic chip

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CN101132852A (en) * 2005-03-04 2008-02-27 巴斯福股份公司 Microscapsule powder

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101132852A (en) * 2005-03-04 2008-02-27 巴斯福股份公司 Microscapsule powder

Cited By (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105013544B (en) * 2014-04-24 2016-11-23 中国科学院青岛生物能源与过程研究所 A kind of microlayer model fusion method based on the induction of hydrophilic fibers silk
CN104148124B (en) * 2014-07-30 2015-12-16 河北工业大学 A kind of drop formation device for micro-fluidic chip
CN104148124A (en) * 2014-07-30 2014-11-19 河北工业大学 Droplet formation device for micro-fluidic chips
CN105013547A (en) * 2015-07-30 2015-11-04 天津大学 Novel microbubble/liquid drop generation regulation and control device and novel microbubble/liquid drop generation regulation and control method
CN105233893A (en) * 2015-11-02 2016-01-13 华东理工大学 Method for preparing micro-droplets based on micro-fluidic chip modification technology
CN105524829A (en) * 2015-11-25 2016-04-27 成都赫尔墨斯科技有限公司 Micro-fluidic chip for manufacturing tissue engineering micromodule
CN105498875A (en) * 2016-01-27 2016-04-20 杭州霆科生物科技有限公司 Centrifugal micro-fluidic chip for preparing liquid drops
CN105618167A (en) * 2016-01-27 2016-06-01 杭州霆科生物科技有限公司 Centrifugal microfluidic chip for preparing droplets in high-throughput manner
CN106110340A (en) * 2016-07-12 2016-11-16 陈传品 A kind of method and device preparing target drug-carrying microbubble
CN106110340B (en) * 2016-07-12 2019-09-03 陈传品 A kind of method and device preparing target drug-carrying microbubble
CN106076446A (en) * 2016-08-19 2016-11-09 北京工业大学 A kind of pair of branch road realizes the microchannel of interval microlayer model fusion function
CN106076446B (en) * 2016-08-19 2018-12-07 北京工业大学 A kind of double branches realize the microchannel of interval microlayer model fusion function
CN106378217A (en) * 2016-12-06 2017-02-08 太原理工大学 Micro-fluidic chip capable of producing two kinds of droplets including oil-water-oil droplets and water-oil-water droplets
CN109294866A (en) * 2017-07-24 2019-02-01 广州康昕瑞基因健康科技有限公司 Emulsion chip and emulsion preparing device
CN107583693A (en) * 2017-08-30 2018-01-16 武汉科技大学 A kind of T-shaped microchannel integrates droplet generation chip
CN108043483A (en) * 2018-02-05 2018-05-18 东南大学 High-throughput drop formation and collection device based on density adjusting
CN108043483B (en) * 2018-02-05 2020-02-18 东南大学 High-flux liquid drop generating and collecting device based on density regulation
WO2020082487A1 (en) * 2018-10-23 2020-04-30 深圳市博瑞生物科技有限公司 Microfluidic chip
CN111013676A (en) * 2019-12-17 2020-04-17 江苏圣极基因科技有限公司 Liquid drop preparation method and micro-fluidic chip

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Application publication date: 20131113