CN101275926B - Special micro-flow control chip and single sampling continuous separation electrophoresis method - Google Patents
Special micro-flow control chip and single sampling continuous separation electrophoresis method Download PDFInfo
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- CN101275926B CN101275926B CN 200710159328 CN200710159328A CN101275926B CN 101275926 B CN101275926 B CN 101275926B CN 200710159328 CN200710159328 CN 200710159328 CN 200710159328 A CN200710159328 A CN 200710159328A CN 101275926 B CN101275926 B CN 101275926B
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- 238000001962 electrophoresis Methods 0.000 title claims abstract description 29
- 238000000926 separation method Methods 0.000 title claims abstract description 21
- 238000000034 method Methods 0.000 title claims abstract description 20
- 238000005070 sampling Methods 0.000 title claims abstract description 12
- 239000007788 liquid Substances 0.000 claims description 42
- 239000012530 fluid Substances 0.000 claims description 11
- 238000013016 damping Methods 0.000 claims description 10
- 239000000872 buffer Substances 0.000 claims description 7
- 239000000126 substance Substances 0.000 claims description 5
- 239000004205 dimethyl polysiloxane Substances 0.000 claims description 3
- 235000013870 dimethyl polysiloxane Nutrition 0.000 claims description 3
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- 239000000463 material Substances 0.000 claims description 3
- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 claims description 3
- 238000004987 plasma desorption mass spectroscopy Methods 0.000 claims description 3
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 claims description 3
- 229920003229 poly(methyl methacrylate) Polymers 0.000 claims description 3
- 239000004926 polymethyl methacrylate Substances 0.000 claims description 3
- 238000003672 processing method Methods 0.000 claims description 3
- 230000015572 biosynthetic process Effects 0.000 claims description 2
- 239000000523 sample Substances 0.000 abstract description 42
- 238000002474 experimental method Methods 0.000 abstract description 9
- 238000010790 dilution Methods 0.000 abstract description 4
- 239000012895 dilution Substances 0.000 abstract description 4
- 239000007853 buffer solution Substances 0.000 abstract description 3
- 239000012488 sample solution Substances 0.000 abstract 2
- 239000000243 solution Substances 0.000 abstract 2
- 230000009286 beneficial effect Effects 0.000 abstract 1
- 239000003814 drug Substances 0.000 abstract 1
- 230000005611 electricity Effects 0.000 abstract 1
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- 239000002699 waste material Substances 0.000 description 6
- 238000001514 detection method Methods 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 4
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- 238000012216 screening Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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Abstract
The present invention discloses a special microfluidic chip and a single-sampling continuous-separating electrophoresis method. The invention is applied in the technique field of the microfluidic chip in life science, medicine and analytical chemistry. The invention is characterized in that the invention comprises a method for exerting the electrophoresis high tension electricity parameter and a microfluidic chip which is used together with the method. The shunting driving voltage which is exerted to the microfluidic chip through an electrophoresis special high voltage power source leads to that the redundant buffer solution and sample solution totally flow into the shunting solution storage tank through a shunting channel. The side wall of the sampling channel on the special microfluidic chip is added with a shunting channel which forms a crossing structure with the former sampling channel and a shunting solution storage tank communicating with the shunting channel. The beneficial effects of the invention is effective prevention to the leakage of the buffer solution, avoidance to the dilution of the sample solution, evident reinforcement to the repeatability of a plurality of peak heights of the sample in the continuous-separation electrophoresis pattern and increased accuracy of the analyzing result of the continuous-electrophoresis separation experiment.
Description
Technical field
The invention belongs to the micro-fluidic chip technical field that is applied in life science, medical science, analytical chemistry, relate to a kind of micro-fluidic chip special and single sampling continuous separation electrophoresis method thereof, specially refer to a kind of electrophoresis method that can improve peak height stability in the continuous separation electrophoresis collection of illustrative plates and the micro-fluidic chip that cooperates the method to use.
Background technology
Micro-fluidic chip (Microfluidic Chip) claims that again (Micro Total AnalysisSystem, μ-TAS) are the technology on the chip of process integration to a number square centimeter sizes such as the sample preparation that will relate in the field such as chemical, biological, biochemical reaction, separation and detection to micro-total analysis system.When electrophoretic separation, little raceway groove two ends apply voltage, flow direction and the speed of control fluid in little raceway groove under electrophoresis and electroosmotic flow compound action, thereby the compartment analysis of each component of realization sample.Than traditional Capillary Electrophoresis, the electrophoretic separation on the micro-fluidic chip with its separate fast, efficient is high, sample consumption less, the plurality of advantages such as automaticity height is widely used in the fields such as chemical detection, bio-separation and drug development.Especially when carrying out the work of a large amount of reagent screenings and reagent detection, the single sample introduction continuous electrophoresis of micro-fluidic chip separates can significantly improve detection efficiency, shortens detection time, saves testing cost.
But when carrying out the continuous electrophoresis separating experiment with the extraining sampling method that generally adopts at present on the micro-fluidic chip, can produce damping fluid leaks, damping fluid enters the sample liquid pool by the sample introduction raceway groove, cause the dilution of sample liquid, cause that repeatedly sample peak height fluctuation range is very large in the electrophoresis pattern, repeatedly peak height stability is very poor, is unfavorable for the component content in the accurate calculation sample, is not easy to fast, accurately, in batches and economical carry out the continuous separation electrophoresis experiment.
Summary of the invention
The purpose of this invention is to provide and a kind ofly can significantly improve in the single sampling continuous separation electrophoresis collection of illustrative plates the repeatedly new method of peak height stability of sample, and the micro-fluidic chip that cooperates the method to use.
Technical scheme of the present invention is:
A kind of micro-fluidic chip special at the shunting liquid storage tank that the increase of sample introduction trench sidewalls place is shunted raceway groove and is communicated with it, is shunted crossing symmetry or the asymmetric bifurcation structure of forming of raceway groove and former sample introduction raceway groove take traditional micro-processing method as the basis; Width and the degree of depth of the little raceway groove of each bar are 40 microns to 100 microns in the micro-fluidic chip, adopt PMMA, PDMS polymkeric substance or glass material to be made.
A kind of single sampling continuous separation electrophoresis method of micro-fluidic chip special, when on micro-fluidic chip special, sample liquid being carried out continuous separation electrophoresis,, make respectively by buffer pool and sample liquid pool and enter damping fluid unnecessary in the sample introduction raceway groove and sample liquid all flows into the shunting liquid storage tank by the shunting raceway groove the shunting driving voltage that micro-fluidic chip special applies by high-voltage power supply special.
Effect of the present invention and benefit are can effectively stop damping fluid to leak to enter the sample liquid pool, thereby avoid dilution that sample liquid is caused, can obviously strengthen in the continuous separation electrophoresis collection of illustrative plates the repeatedly repeatability of peak height of sample, improve continuous electrophoresis separating experiment precision of analysis.
Description of drawings
Fig. 1 is the micro-fluidic chip special structural representation.
Fig. 2 is output electrode inserting mode synoptic diagram on micro-fluidic chip special of high-voltage power supply special.
Fig. 3 is the synoptic diagram of the interior liquid flow direction of little raceway groove in the sample introduction process.
Fig. 4 is the synoptic diagram of the interior liquid flow direction of little raceway groove in the detachment process.
Among the figure: 1 sample liquid pool; 2 buffer pools; 3 sample waste liquid pools; 4 damping fluid waste liquid pools; 5 shunting liquid storage tanks; 6 shunting raceway grooves; 7 separate raceway groove; 8 lower floor's substrates; 9 upper strata cover plates.
Embodiment
Describe the specific embodiment of the present invention in detail below in conjunction with technical scheme and accompanying drawing.
As shown in Figure 1, micro-fluidic chip special is take traditional micro-processing method as the basis, this chip is comprised of upper strata cover plate 9 and lower floor's substrate 8, and the diameter that distributing on the upper strata cover plate 9 is 3 millimeters sample liquid pool 1, buffer pool 2, sample waste liquid pool 3, damping fluid waste liquid pool 4, shunting liquid storage tank 5; Many little raceway grooves are distributing on lower floor's substrate 8, its intersection region is double-T shaped or cross, set up one section shunting raceway groove 6 and intersect the formation bifurcation structure with former sample introduction raceway groove in the sample introduction trench sidewalls, this bifurcation structure can make shunting raceway groove 6 and angled symmetry or the asymmetric distribution of former sample introduction raceway groove, and its angular dimension is looked the experiment situation and is chosen between 30 ° to 150 °; After above-mentioned upper strata cover plate 9 and lower floor's substrate 8 be bonded to one, each liquid storage tank was interconnected by little raceway groove, and every little raceway groove end all is in the corresponding liquid storage tank.Width and the degree of depth of the little raceway groove of each bar are 40 microns to 100 microns in the micro-fluidic chip, and the making material is PMMA, PDMS polymkeric substance or glass.
The inserting mode of four road high-voltage output electrodes on micro-fluidic chip special of electrophoresis high-voltage power supply special as shown in Figure 2, carry out electrophoresis experiment by high-voltage power supply at the electric field that micro-fluidic chip applies, complete electrophoresis experiment is divided into two processes---sample introduction with separate.At first, in sample liquid pool 1, inject the sample liquid that to analyze, all inject appropriate amount of buffer solution in other liquid storage tanks and the little raceway groove.Then adopt the method control sample introduction of conventional electronic extraining sampling, namely in sample liquid pool 1, apply certain sample introduction voltage, in buffer pool 2 and damping fluid waste liquid pool 4, apply corresponding folder stream voltage, sample waste liquid pool 3 and shunting liquid storage tank 5 are unsettled, and the flow direction of the interior liquid of little raceway groove as shown in Figure 3 during sample introduction.Begin afterwards detachment process, the voltage that applies in each liquid storage tank is changed simultaneously, apply separation voltage in the buffer pool 2, and apply corresponding shunting voltage in the sample cell 1, all the other liquid storage tanks are all unsettled, to enter the unnecessary damping fluid of sample introduction raceway groove by buffer pool 2 and sample liquid pool 1 respectively and sample liquid all flows into shunting liquid storage tanks 5 by shunting raceway groove 6, the flow direction of the interior liquid of little raceway groove as shown in Figure 4 during separation.
The single sampling continuous separation electrophoresis method that adopts the present invention to propose cooperates micro-fluidic chip special to carry out the electrophoretic analysis experiment, can effectively stop damping fluid to leak and enter sample liquid pool 1, avoid dilution that sample liquid is caused, thereby obviously strengthen in the continuous separation electrophoresis collection of illustrative plates the repeatedly repeatability of peak height of sample, improve continuous electrophoresis separating experiment precision of analysis.
Claims (1)
1. use a kind of single sampling continuous separation electrophoresis method of micro-fluidic chip special, described micro-fluidic chip special, take traditional micro-processing method as the basis, the shunting liquid storage tank (5) that increases shunting raceway groove (6) and be communicated with it at sample introduction trench sidewalls place, shunting raceway groove (6) and former sample introduction raceway groove intersect the symmetrical or asymmetric bifurcation structure of formation, and angle is 30 ~ 150 degree between two shunting raceway grooves; Width and the degree of depth of the little raceway groove of each bar are 40 microns to 100 microns in the micro-fluidic chip, adopt PMMA, PDMS polymkeric substance or glass material to be made; It is characterized in that: when on micro-fluidic chip special, sample liquid being carried out continuous separation electrophoresis,, make respectively to enter damping fluid unnecessary in the sample introduction raceway groove and sample liquid by buffer pool (2) and sample liquid pool (1) and all flow into by shunting raceway groove (6) and shunt liquid storage tank (5) to the shunting driving voltage that micro-fluidic chip special applies by high-voltage power supply special.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN 200710159328 CN101275926B (en) | 2007-12-29 | 2007-12-29 | Special micro-flow control chip and single sampling continuous separation electrophoresis method |
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| CN 200710159328 CN101275926B (en) | 2007-12-29 | 2007-12-29 | Special micro-flow control chip and single sampling continuous separation electrophoresis method |
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| CN101275926A CN101275926A (en) | 2008-10-01 |
| CN101275926B true CN101275926B (en) | 2013-04-17 |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102023146A (en) * | 2010-09-28 | 2011-04-20 | 上海理工大学 | Electrophoretic chip for optical detection and electrophoretic device thereof |
| CN102728420B (en) * | 2011-04-13 | 2014-07-02 | 白向阳 | Heterogeneous inverted flow chip and preparation method thereof |
| CN104772168B (en) * | 2015-03-19 | 2017-01-04 | 华南理工大学 | The micro-fluidic chip of a kind of microfluid spontaneous vasomotion and manufacture method, priming device |
| CN105688721B (en) * | 2016-01-06 | 2018-01-12 | 中国计量学院 | For generating the micro-fluidic chip of spherical microbubble |
| CN109647557B (en) * | 2019-02-27 | 2021-04-02 | 哈尔滨工业大学 | Direct particle separation chip based on induced charge electroosmosis micro vortex and application and separation method thereof |
| CN110270386B (en) * | 2019-04-11 | 2021-06-04 | 上海天马微电子有限公司 | Microfluidic chip and driving method thereof |
| EP4141098A4 (en) * | 2021-01-29 | 2023-11-22 | BOE Technology Group Co., Ltd. | Device and method for driving microfluidic chip |
Citations (5)
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| WO2005026742A1 (en) * | 2003-09-12 | 2005-03-24 | Nec Corporation | Chip, device using the chip, and method of using the chip |
| US6994826B1 (en) * | 2000-09-26 | 2006-02-07 | Sandia National Laboratories | Method and apparatus for controlling cross contamination of microfluid channels |
| CN1737562A (en) * | 2005-06-27 | 2006-02-22 | 浙江大学 | Micro flow control chip capillary electrophoresis negative pressure sampling method |
| CN1804633A (en) * | 2006-01-17 | 2006-07-19 | 浙江大学 | Microfluidic analysis chip employing liquid-liquid extraction and capillary electrophoresis and preparation method thereof |
| JP4366523B2 (en) * | 2003-10-03 | 2009-11-18 | 財団法人生産技術研究奨励会 | Electrophoresis chip and sample analysis method using the same |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6994826B1 (en) * | 2000-09-26 | 2006-02-07 | Sandia National Laboratories | Method and apparatus for controlling cross contamination of microfluid channels |
| WO2005026742A1 (en) * | 2003-09-12 | 2005-03-24 | Nec Corporation | Chip, device using the chip, and method of using the chip |
| JP4366523B2 (en) * | 2003-10-03 | 2009-11-18 | 財団法人生産技術研究奨励会 | Electrophoresis chip and sample analysis method using the same |
| CN1737562A (en) * | 2005-06-27 | 2006-02-22 | 浙江大学 | Micro flow control chip capillary electrophoresis negative pressure sampling method |
| CN1804633A (en) * | 2006-01-17 | 2006-07-19 | 浙江大学 | Microfluidic analysis chip employing liquid-liquid extraction and capillary electrophoresis and preparation method thereof |
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| Shu-Hui Chen等.Flow-Through Sampling for Electrophoresis-Based Microchips and Their Applications for Protein Analysis.《Anal. Chem.》.2002,第74卷(第19期),5146-5153. * |
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