CN101708439B - Chaotic microfluidic chip mixer and mixing method thereof - Google Patents
Chaotic microfluidic chip mixer and mixing method thereof Download PDFInfo
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- CN101708439B CN101708439B CN2009101541278A CN200910154127A CN101708439B CN 101708439 B CN101708439 B CN 101708439B CN 2009101541278 A CN2009101541278 A CN 2009101541278A CN 200910154127 A CN200910154127 A CN 200910154127A CN 101708439 B CN101708439 B CN 101708439B
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- microchannel
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- microfluidic chip
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- 230000000739 chaotic effect Effects 0.000 title claims abstract description 14
- 238000002156 mixing Methods 0.000 title claims abstract description 10
- 238000000034 method Methods 0.000 title claims description 11
- 239000000758 substrate Substances 0.000 claims abstract description 26
- 238000007789 sealing Methods 0.000 claims description 3
- 239000000243 solution Substances 0.000 abstract description 26
- 230000000694 effects Effects 0.000 abstract description 14
- 238000012545 processing Methods 0.000 abstract description 9
- 238000006243 chemical reaction Methods 0.000 abstract description 8
- 239000011259 mixed solution Substances 0.000 abstract description 4
- 238000004458 analytical method Methods 0.000 abstract description 3
- 238000001514 detection method Methods 0.000 abstract description 3
- 238000005516 engineering process Methods 0.000 abstract description 2
- 238000012544 monitoring process Methods 0.000 abstract description 2
- 230000007613 environmental effect Effects 0.000 abstract 1
- 238000002835 absorbance Methods 0.000 description 5
- 238000013461 design Methods 0.000 description 4
- 239000010453 quartz Substances 0.000 description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 4
- 239000012153 distilled water Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 230000001419 dependent effect Effects 0.000 description 2
- 230000005684 electric field Effects 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000035484 reaction time Effects 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- KSPIHGBHKVISFI-UHFFFAOYSA-N Diphenylcarbazide Chemical compound C=1C=CC=CC=1NNC(=O)NNC1=CC=CC=C1 KSPIHGBHKVISFI-UHFFFAOYSA-N 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 229920006335 epoxy glue Polymers 0.000 description 1
- 238000005530 etching Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000007731 hot pressing Methods 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 230000036632 reaction speed Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000001039 wet etching Methods 0.000 description 1
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Abstract
The invention discloses a chaotic microfluidic chip mixer comprising a first substrate and a second substrate which are sealed in together. The chaotic microfluidic chip mixer is characterized in that the upper surface of the first substrate is provided with a microchannel, at least two vertical through holes are arranged at interval on the second substrate along horizontal direction and are communicated with the microchannel; solution passes through the through hole and successively enters the microchannel along the direction vertical to the microchannel, different solutions are vertically mixed in the mixing zone of the microchannel to produce the chaotic effect and avoid the laminar flow effect between solutions, so that the chaotic microfluidic chip mixer instantly mixes different solutions and accelerates the mixed solutions to quickly finish reaction. The microfluidic chip mixer prepared by the invention has simple technology, does not need expensive processing equipment and can be widely applied to the analysis and detection field of environmental monitoring, life science and the like.
Description
Technical field
The present invention relates to the design and the processing of micro-mixer, particularly relate to the design and the processing of chaotic microfluidic chip mixer.
Background technology
Based on the biological and chemical of micro-fluidic chip reaction have reagent consumption less, fast, the productive rate advantages of higher of reaction speed; DNA hybridization, cell-stimulating, enzyme reaction, organic synthesis and the analytical test etc. that on micro-fluidic chip, often carry out relate to the mixing of reactant inevitably.Rapid and uniform is mixed significant for the microfluidic system in fields such as biochemical reaction, organic synthesis and analytical test.Because the size of micro-fluidic chip passage is little, the laminar flow effect in the passage is remarkable, had a strong impact between the solution diffusion with mix, thereby the carrying out of influence reaction.
Mostly the microfluidic chip mixer of report is in the microchannel of processing more than two on the micro-fluidic chip at present; Pool a hybrid reaction passage then; Solution produces in the hybrid reaction passage after the laminar flow effect, destroys the laminar flow effect between solution through blender, realizes mixing.According to the action mode of blender, microfluidic chip mixer is divided into active mixer and passive blender.Active mixer produces by external force such as magnetic force, electric field force, sound fields mixes.Passive blender relies on the geometry of passage or fluid behaviour to produce mixed effect merely, does not comprise movable part.Chaos mixer is typical case's representative of passive blender, and normally little processing ridge projection in the bottom, microchannel of micro-fluidic chip is destroyed the laminar flow effect between solution.In general; Passive and active mixer needs complicated little process and expensive process equipment usually; Processing, micro-fluidic chip and the electric field of micro-structural in the for example processing of three dimension microchannels, passage, magnetic field, sound field integrated etc., thus limited its application.
Summary of the invention
The purpose of this invention is to provide a kind of chaotic microfluidic chip mixer, use this blender can avoid different solutions when mixing, to produce the laminar flow effect.
For realizing the foregoing invention purpose; The technical scheme that the present invention taked is: this chaotic microfluidic chip mixer comprises first substrate and second substrate of sealing; Wherein, be provided with the microchannel at the upper surface of first substrate, the degree of depth of said microchannel is 10～100 microns; The along continuous straight runs compartment of terrain is provided with two vertical through holes at least and different solutions is produced vertically cross on second substrate, and said through hole is communicated with the microchannel.
Further, the degree of depth of microchannel according to the invention is 10～50 microns.
Further, microchannel according to the invention is shaped as linear, broken line shape or spirality.
Using micro-mixer of the present invention to carry out method of mixing is that different solution is introduced in the microchannel through isolated two the above through holes of along continuous straight runs respectively, described different solutions is vertically crossed in the microchannel, and mix.
Compared with prior art; The invention has the beneficial effects as follows: different solutions vertically gets into the microchannel successively; Mixed zone in the microchannel vertically crosses; Original solution in the narrow and small microchannel of the vertical fluid challenge that gets into (degree of depth of microchannel can at the 10-100 micron), the generation chaos effect, thus avoided the generation of laminar flow effect between the solution; If the degree of depth of microchannel is the 10-50 micron, then can prevent the generation of laminar flow effect between the solution better.Chaos mixer of the present invention has also been avoided the processing of bottom micro-structural of the microchannel of existing chaotic microfluidic chip mixer; Chaos mixer process of the present invention is simple, and mixing velocity is fast, need not to use expensive process equipment, can in common lab, accomplish processing and fabricating.
Description of drawings
The mixing principle sketch map of Fig. 1 chaotic microfluidic chip mixer of the present invention;
The structural representation of Fig. 2 broken line type microchannel microfluidic chip mixer;
The structural representation of Fig. 3 linear pattern microchannel microfluidic chip mixer;
The linear relationship curve map of the absorbance of Fig. 4 liquor potassic permanganate and concentration;
The linear relationship curve map of Fig. 5 Cr VI solution absorbency and concentration;
Among the figure: 1-first substrate, 2-microchannel, 3-second substrate, 4-first through hole, 5-mixed zone, 6-second through hole, 7-solution sample introduction interface, 8-quartz capillary.
The specific embodiment
The mixing principle sketch map of blender of the present invention is referring to Fig. 1.
As shown in Figure 2, etching or hot pressing length are that 40 centimetres, width are that 100 microns, the degree of depth are 10 microns the single microchannel 2 of broken line type on first substrate 1.3 vertical through holes are bored in the along continuous straight runs compartment of terrain on second substrate 3, and first substrate 1 and second substrate 3 are sealed, and two first through holes 4 and one second through hole 6 on second substrate 3 are communicated with microchannel 2; Wherein, the outlet of the microchannel 2 among Fig. 2 and 6 handing-over of second through hole make mixed solution flow out micro-mixer through second through hole 6 again by microchannel 2.
Different solution is under the driving of normal pressure or negative pressure; The edge gets into microchannel 2 through two first through holes 4 respectively successively with microchannel 2 vertical directions; 2 mixed zone 5 vertically crosses and produces chaos effect in the microchannel; Avoided the generation of laminar flow effect, made to be blended in the moment completion, mixed solution flows out micro-mixers through second through hole 6.
The material of substrate can be glass, quartz or polymer, and the material of first substrate 1 and second substrate 3 can be the same or different.According to the size of micro-fluidic chip and the length of required microchannel 2, can the shaped design of microchannel 2 be in line, different shape such as broken line or spiral line type.
As shown in Figure 3, the wet etching degree of depth is that 30 microns, length are that 3 centimetres, width are 200 microns single linear pattern microchannel 2 on first substrate 1; To bore the level interval of vertical 4, two first through holes 4 of first through hole of 500 microns of 2 diameters be 1 centimetre in the along continuous straight runs compartment of terrain on second substrate 3.First substrate 1 and second substrate, 3 high temperature are sealed, make two first through holes 4 all aim at microchannel 2, and two first through holes 4 are communicated with microchannel 2.At first through hole, 4 place's adhesive solution sample introduction interfaces 7, solution sample introduction interface 7 links to each other with syringe pump through capillary.Present embodiment is not provided with second through hole 6 as shown in Figure 2 like embodiment 1; But 2 end bores 0.4 millimeter of a diameter in the microchannel, a quartz capillary 8 is also therefrom inserted in dark 2 millimeters hole; To export as mixed solution, this quartz capillary 8 uses epoxy glues sealing.A syringe pump is 2.5 * 10 through one of them first through hole 4 implantation concentration in blender
-4The liquor potassic permanganate of mol/L; Another syringe pump injects distilled water through another first through hole 4 in blender; Liquor potassic permanganate and distilled water is 2 mixed zone 5 vertical crossing and produce chaos effect in the microchannel; Can know that through microscopically Real Time Observation mixed process mixed process was accomplished in moment.The liquor potassic permanganate that blender the is flowed out detection cell that circulates detects absorbance in real time.Continuously change the flow of distilled water and liquor potassic permanganate, make the liquor potassic permanganate theoretical concentration of outflow be respectively 2.5 * 10
-4Mol/L, 1.67 * 10
-4Mol/L, 1.25 * 10
-4Mol/L, 0.083 * 10
-4Mol/L and 0mol/L, total flow is 30 μ l/min.Liquor potassic permanganate absorbance and concentration linear relationship curve are as shown in Figure 4, and its linearly dependent coefficient reaches 0.997, explain that this micro-mixer has excellent mixed effect.
According to the size of micro-fluidic chip and the length of required microchannel 2, can the shaped design of microchannel 2 be in line, different shape such as broken line or spiral line type.
The instance of an online reaction is provided according to embodiment 2.As shown in Figure 3; The degree of depth that adopts a microchannel 2 is that 50 microns, length are that 3 centimetres, width are 200 microns linear pattern microchannel micro-mixer; A syringe pump injects Cr VI solution in blender, another syringe pump injects developer diphenylcarbazide solution in blender, and fixedly the flow of developer is 15 μ l/min; Injecting mixer Cr VI solution concentration is respectively 0.125mg/L, 0.25mg/L, 0.50mg/L, 0.75mg/L and 1.00mg/L; Flow is 15 μ l/min, and the solution that blender the flows out detection cell that circulates detects absorbance in real time.Cr VI solution absorbance and concentration linear relationship curve are as shown in Figure 5, and its linearly dependent coefficient reaches 0.998.Can know that by Fig. 5 the micro-mixer that this instance provides can satisfy the requirement of online reaction and on-line monitoring, for long reaction of reaction time can be through prolonging micro-mixer microchannel 2, reduce methods such as flow velocity or online heating and prolong the reaction time.
Claims (4)
1. chaotic microfluidic chip mixer; It comprises first substrate (1) and second substrate (3) of sealing; It is characterized in that: the upper surface at first substrate (1) is provided with microchannel (2); The degree of depth of said microchannel (2) is 10～100 microns, goes up the along continuous straight runs compartment of terrain at second substrate (3) and is provided with two vertical through holes (4) at least, and said through hole (4) is communicated with microchannel (2).
2. a kind of chaotic microfluidic chip mixer according to claim 1 is characterized in that: the degree of depth of said microchannel (2) is 10～50 microns.
3. a kind of chaotic microfluidic chip mixer according to claim 1 is characterized in that: said microchannel (2) be shaped as linear, broken line shape or spirality.
4. one kind is used the micro-mixer of claim 1 to carry out method of mixing; It is characterized in that: different solution is introduced in the microchannel (2) through isolated two the above through holes of along continuous straight runs (4) respectively; Described different solutions is vertically crossed in microchannel (2), and mix.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2009101541278A CN101708439B (en) | 2009-11-05 | 2009-11-05 | Chaotic microfluidic chip mixer and mixing method thereof |
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| CN2009101541278A CN101708439B (en) | 2009-11-05 | 2009-11-05 | Chaotic microfluidic chip mixer and mixing method thereof |
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| CN101708439A CN101708439A (en) | 2010-05-19 |
| CN101708439B true CN101708439B (en) | 2012-04-25 |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101957274A (en) * | 2010-08-24 | 2011-01-26 | 苏州聚阳环保科技有限公司 | Multichannel block for taking liquid |
| JP5395861B2 (en) * | 2011-09-09 | 2014-01-22 | 株式会社神戸製鋼所 | Channel structure and method for manufacturing channel structure |
| CN104162458B (en) * | 2013-05-16 | 2017-11-14 | 昌微系统科技(上海)有限公司 | A kind of microfluidic device for fluid detection and the method for preparing the microfluidic device |
| CN110732355B (en) * | 2019-09-27 | 2021-09-28 | 东南大学 | Micro-mixing micro-fluidic chip |
| CN110732275A (en) * | 2019-10-15 | 2020-01-31 | 武夷学院 | laminated passive micromixer and its making method |
| WO2023018845A1 (en) * | 2021-08-12 | 2023-02-16 | Academia Sinica | Microfluidic mixer for enhanced three-dimensional mixing |
| CN116747917A (en) * | 2023-04-26 | 2023-09-15 | 上海科技大学 | Microfluidic chip and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5921678A (en) * | 1997-02-05 | 1999-07-13 | California Institute Of Technology | Microfluidic sub-millisecond mixers |
| CN2650109Y (en) * | 2003-11-06 | 2004-10-20 | 浙江大学 | Three-dimensional cross-guiding miniature mixer |
| CN1542428A (en) * | 2003-11-04 | 2004-11-03 | 浙江大学 | Multiple input rotational flow field minisize agitator |
| CN1542429A (en) * | 2003-11-06 | 2004-11-03 | 浙江大学 | Cross flow guiding type minisize static agitator |
| CN101234324A (en) * | 2007-11-21 | 2008-08-06 | 清华大学 | Micro-contact mixer |
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2009
- 2009-11-05 CN CN2009101541278A patent/CN101708439B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5921678A (en) * | 1997-02-05 | 1999-07-13 | California Institute Of Technology | Microfluidic sub-millisecond mixers |
| CN1542428A (en) * | 2003-11-04 | 2004-11-03 | 浙江大学 | Multiple input rotational flow field minisize agitator |
| CN2650109Y (en) * | 2003-11-06 | 2004-10-20 | 浙江大学 | Three-dimensional cross-guiding miniature mixer |
| CN1542429A (en) * | 2003-11-06 | 2004-11-03 | 浙江大学 | Cross flow guiding type minisize static agitator |
| CN101234324A (en) * | 2007-11-21 | 2008-08-06 | 清华大学 | Micro-contact mixer |
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