CN107677533B - Slide soaking and cleaning device - Google Patents
Slide soaking and cleaning device Download PDFInfo
- Publication number
- CN107677533B CN107677533B CN201711018043.2A CN201711018043A CN107677533B CN 107677533 B CN107677533 B CN 107677533B CN 201711018043 A CN201711018043 A CN 201711018043A CN 107677533 B CN107677533 B CN 107677533B
- Authority
- CN
- China
- Prior art keywords
- slide
- soaking
- frame
- glass
- cleaning
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000002791 soaking Methods 0.000 title claims abstract description 57
- 238000004140 cleaning Methods 0.000 title claims abstract description 45
- 239000011521 glass Substances 0.000 claims abstract description 46
- 238000002474 experimental method Methods 0.000 claims abstract description 5
- 239000007788 liquid Substances 0.000 claims description 25
- 238000005406 washing Methods 0.000 claims description 4
- 239000002351 wastewater Substances 0.000 claims description 3
- 238000001802 infusion Methods 0.000 claims 8
- 238000000034 method Methods 0.000 abstract description 8
- 244000005700 microbiome Species 0.000 description 6
- 230000001580 bacterial effect Effects 0.000 description 4
- 230000034994 death Effects 0.000 description 4
- 239000012634 fragment Substances 0.000 description 4
- 238000013467 fragmentation Methods 0.000 description 4
- 238000006062 fragmentation reaction Methods 0.000 description 4
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 3
- 239000010931 gold Substances 0.000 description 3
- 229910052737 gold Inorganic materials 0.000 description 3
- 238000011065 in-situ storage Methods 0.000 description 3
- 229920000936 Agarose Polymers 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 230000030833 cell death Effects 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 108010077544 Chromatin Proteins 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000006907 apoptotic process Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 210000003483 chromatin Anatomy 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 125000001475 halogen functional group Chemical group 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000002934 lysing effect Effects 0.000 description 1
- 210000004940 nucleus Anatomy 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/04—Cleaning involving contact with liquid
- B08B3/048—Overflow-type cleaning, e.g. tanks in which the liquid flows over the tank in which the articles are placed
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a slide soaking and cleaning device, which comprises a slide cleaning tank and a slide soaking clamp, wherein an angle adjusting piece of the slide soaking clamp is connected with a lifting device arranged on the slide cleaning tank; the inclined angle of the glass slide can be finely and stably controlled through the angle adjusting piece, so that the solution can be gently removed, fragmented DNA drifting is avoided, errors are caused to experimental results, and meanwhile, the consumption of soaking and cleaning solutions is reduced, and the solution is easy to recycle after the experiment; the lifting device comprises a rotating gear, a lifting rod and a supporting piece, wherein the lifting rod is meshed with the rotating gear, a driving shaft of the rotating gear is arranged on the outer wall of the glass slide cleaning pool through a bearing, a guide block used for the lifting rod to pass through is fixed on the outer wall of the glass slide cleaning pool, and the angle adjusting piece is connected onto the supporting piece in a shaft mode, so that the device can suspend or stick the glass slide to the bottom, and each step of the experimental process can be operated according to requirements.
Description
Technical Field
The invention relates to the technical field of laboratory auxiliary equipment, in particular to a glass slide soaking and cleaning device.
Background
Bacterial cell death may be a programmed process similar to the phenomenon of apoptosis in higher organisms, such as bacterial death after the action of bactericidal antibiotics. Paper [ Jos e Luis fernandez, mwave Cartelel, lourdes Muriel, et al dna Fragmentation in Microorganisms Assessed In Situ [ J ]. Applied and environmental microbiology,2008, 74 (19): 5925-5933 and chinese patent publication No. CN101589156a, methods for determining DNA fragmentation of microorganisms, describe methods for in situ investigation of chromatin/DNA integrity of microorganisms to assess bacterial death, which methods are particularly focused on death caused by DNA degradation or DNA degradation caused by death. Cells immersed in an inert agarose gel on a slide were lysed: if the cell has fragmented DNA, the fragments will diffuse from the original nucleus into the agarose medium, so that a broad halo due to the diffusion of the DNA fragments to the surroundings can be observed; the use of highly sensitive fluorescent dyes, such as SYBRgold (SYBR golden stain), is able to distinguish between the nuclear-like DNA fibers of microorganisms, and in particular can accurately reveal small DNA fragments that have been diffused into agarose during such DNA fragmentation events. In this way it is possible to obtain a relatively simple and fast tool which enables in situ assessment of DNA breaks in bacteria and other microorganisms on a cell-by-cell basis in a reproducible manner; after the action of the lysing agent, bacterial cell death can be rapidly analyzed by observing the fragmented DNA molecules at the microscopic level.
In the experimental process, the soaking and cleaning of the glass slide should be operated at a horizontal position as much as possible, and the operation of removing the solution on the glass slide should be gentle so as to avoid fragmented DNA drift and error on experimental results. Under the existing experimental conditions, the glass slide needs to be clamped by forceps for soaking and cleaning, and the defects that the glass slide is easy to slide and cannot be subjected to fine operation exist, so that the experimental result is not ideal and the experimental reagent is wasted. There is therefore a strong need for an experimental handling device for a method of DNA fragmentation of microorganisms that is capable of more elaborate handling of the soaking and washing of slides.
Disclosure of Invention
The invention aims to solve the technical problem of providing a slide soaking and cleaning device which can more finely operate slide soaking and cleaning.
The technical scheme adopted for solving the technical problems is as follows: the device comprises a slide cleaning tank and a slide soaking clamp, wherein an angle adjusting piece of the slide soaking clamp is connected with a lifting device arranged on the slide cleaning tank.
The lifting device comprises a rotating gear, a lifting rod meshed with the rotating gear and a supporting piece fixed at the top end of the lifting rod; the driving shaft of the rotating gear is arranged on the outer wall of the glass slide cleaning pool through a bearing, and a guide block for the lifting rod to pass through is fixed on the outer wall of the glass slide cleaning pool; the angle adjusting piece is connected to the supporting piece in a shaft mode. The device can suspend or stick the glass slide to the bottom, so that each step of the experimental process can be operated as required.
Compared with the prior art, the slide glass has the advantages that the slide glass is clamped by the slide glass soaking clamp and is not easy to slide off; the angle of slide glass inclination can be finely and stably controlled through the angle adjusting piece, so that the solution can be gently removed, fragmented DNA drifting is avoided, errors are caused to experimental results, and meanwhile, the consumption of soaking and cleaning liquid is reduced, and the slide glass is easy to recycle after experiments, so that more fine operation is achieved.
As an improvement, the slide soaking clamp comprises a slide flat-laying frame and a clamping piece hinged with the slide flat-laying frame, a torsion spring is arranged between the clamping piece and the slide flat-laying frame, and the slide can be stably clamped in both horizontal and inclined positions; the angle adjusting piece comprises a bent connecting part, an adjusting part and a rotating rod fixed at the free end of the adjusting part, the adjusting part of the angle adjusting piece is connected with the supporting piece in a shaft way, the bottom end of the connecting part of the angle adjusting piece is fixedly connected with the slide glass flat-laying frame, the inclination angle of the slide glass can be finely operated, and the angle adjusting piece is more stable than manual operation, so that the solution can be gently removed.
As an improvement, the head of the clamping piece is hinged with a soaking frame; the bottom of the soaking frame is provided with a groove for embedding the convex blocks on the frame of the experiment area of the slide glass flat-placed frame. The soaking frame ensures that the fixing of the glass slide is firmer, and the solution above the glass slide cannot overflow through the convex blocks, so that the space above the glass slide is reduced, and the consumption of the soaking liquid and the cleaning liquid can be reduced.
As an improvement, the head of the clamping piece is hinged with a cover plate which is coaxial with the soaking frame and is used for covering the soaking frame. When the SYBR Gold is used for dyeing the DNA fragments, the SYBR Gold is easy to decompose by visible light, and the dark environment is favorable for the combination of the DNA and the SYBR Gold; and when the cover plate is closed to slightly shake when the solution is unbalanced, the solution is not easy to overflow.
As an improvement, a soaking liquid collecting ball is arranged on the soaking frame, a liquid collecting block is arranged on the inner side of the soaking frame provided with the soaking liquid collecting ball, a switch valve is arranged on a through hole of the soaking frame communicated with the soaking liquid collecting ball, and a liquid transfer device drawing hole is formed in the upper portion of the soaking liquid collecting ball. When the solution above the slide is removed, it is easily collected and removed in a concentrated manner.
As an improvement, a plurality of rows of tombs are arranged in a glass slide placing area at the bottom of the glass slide cleaning pool, confluence channels are arranged beside the tombs, and other areas of the glass slide cleaning pool are slopes inclining to the glass slide placing area. The holding area is provided with a tommy table, so that the contact area between the slide glass and the bottom can be reduced, and the solution below the slide glass flows more easily, thereby improving the cleaning effect, and enhancing the volatilization of water below the slide glass in the slide glass drying stage; the confluence channel is arranged beside the tombstone, so that waste liquid can be collected intensively when the slide glass is placed at the horizontal position, and the inclination of the slide glass is reduced, so that drift of fragmented DNA is avoided, and experimental results are influenced; the peripheral slope is arranged so that the overflowed solution flows over the slide glass when the solution flows back during cleaning, thereby reducing the consumption of cleaning solution.
As an improvement, a cleaning liquid outlet of the glass slide cleaning tank is provided with a four-way valve, and a liquid outlet of the four-way valve is communicated with the wastewater collection tank. So that the soaking liquid and the cleaning liquid are easier to collect and remove in a concentrated way.
Drawings
Fig. 1 is a schematic elevational view of the present invention.
FIG. 2 is a schematic top view of a slide wash basin of the present invention.
Fig. 3 is a schematic top view of the soaking frame of the present invention.
Fig. 4 is a cross-sectional view A-A of fig. 3.
Detailed Description
The invention is described in further detail below with reference to the embodiments of the drawings.
The preferred embodiment is shown in fig. 1 to 4 as a slide soaking and cleaning apparatus comprising a slide cleaning bath 1 and a slide soaking clamp, the angle adjusting member 24 of the slide soaking clamp being connected to a lifting device mounted on the slide cleaning bath 1.
The lifting device comprises a rotating gear 31, a lifting rod 32 meshed with the rotating gear 31 and a supporting piece 33 fixed at the top end of the lifting rod 32; the driving shaft 35 of the rotating gear 31 is arranged on the outer wall of the slide cleaning pool 1 through a bearing 37, and a guide block 34 for the lifting rod 32 to pass through is fixed on the outer wall of the slide cleaning pool 1; the angle adjusting member 21 is journalled on the support member 33.
The slide soaking clamp comprises a slide flat-placing frame 21 and a clamping piece 22 hinged with the slide flat-placing frame 21, and a torsion spring 23 is arranged between the clamping piece 22 and the slide flat-placing frame 21; the angle adjusting piece 24 is formed by connecting a bent connecting part, an adjusting part and a rotating rod fixed at the free end of the adjusting part, the adjusting part of the angle adjusting piece 24 is connected with the supporting piece 33 in a shaft way, and the bottom end of the connecting part of the angle adjusting piece 24 is fixedly connected with the slide flat-placing frame 21.
The head of the clamping piece 22 is hinged with a soaking frame 25; the bottom of the frame of the soaking frame 25 is provided with a groove 251 for embedding the convex blocks 211 on the frame of the experiment area of the slide flat-placing frame 21.
The head of the clamping piece 22 is hinged with a cover plate 26 which is coaxial with the soaking frame 25 and is used for covering the soaking frame 25.
The soaking frame 25 is provided with a soaking liquid collecting ball 27 on the frame, the inner side of the soaking frame 25 provided with the soaking liquid collecting ball 27 is provided with a liquid collecting block 251, a through hole 252 communicated with the soaking liquid collecting ball 27 on the frame of the soaking frame 25 is provided with a switch valve 28, and the upper part of the soaking liquid collecting ball 27 is provided with a liquid transfer device drawing hole 271.
The slide rest area at the bottom of the slide cleaning pool 1 is provided with a plurality of rows of tombs 11, confluence channels 12 are arranged beside the tombs 11, and other areas of the slide cleaning pool 1 are slopes inclined towards the slide rest area.
The cleaning fluid outlet of the slide cleaning pool 1 is provided with a four-way valve 13, and the liquid outlet of the four-way valve 13 is communicated with the wastewater collection box 4.
Claims (4)
1. A slide glass soaks and belt cleaning device, its characterized in that: the device comprises a glass slide cleaning pool (1) and a glass slide soaking clamp, wherein an angle adjusting piece (24) of the glass slide soaking clamp is connected with a lifting device arranged on the glass slide cleaning pool (1); the slide soaking clamp comprises a slide flat-placing frame (21) and a clamping piece (22) hinged with the slide flat-placing frame (21), and a torsion spring (23) is arranged between the clamping piece (22) and the slide flat-placing frame (21); the angle adjusting piece (24) is formed by connecting a bent connecting part, an adjusting part and a rotating rod fixed at the free end of the adjusting part, the adjusting part of the angle adjusting piece (24) is connected with a supporting piece (33) of the lifting device in a shaft way, and the bottom end of the connecting part of the angle adjusting piece (24) is fixedly connected with the slide flat-placing frame (21);
the head of the clamping piece (22) is hinged with a soaking frame (25); a groove (251) for embedding a convex block (211) on the border of the experiment area of the slide glass flat-placing frame (21) is formed in the bottom of the border of the soaking frame (25);
the head of the clamping piece (22) is hinged with a cover plate (26) which is coaxial with the soaking frame (25) and is used for covering the soaking frame (25); the infusion device is characterized in that an infusion collecting ball (27) is arranged on the frame of the infusion frame (25), a liquid collecting block is arranged on the inner side of the frame of the infusion frame (25) provided with the infusion collecting ball (27), a switch valve (28) is arranged on a through hole (252) formed in the frame of the infusion frame (25) and communicated with the infusion collecting ball (27), and a liquid shifter drawing hole (271) is formed in the upper portion of the infusion collecting ball (27).
2. The slide soaking and washing apparatus according to claim 1, wherein: the lifting device further comprises a rotating gear (31) and a lifting rod (32) meshed with the rotating gear (31), and a supporting piece (33) is fixed at the top end of the lifting rod (32); the driving shaft (35) of the rotating gear (31) is arranged on the outer wall of the slide cleaning pool (1) through a bearing (37), and a guide block (34) used for the lifting rod (32) to penetrate through is fixed on the outer wall of the slide cleaning pool (1).
3. The slide soaking and washing apparatus according to claim 1, wherein: the glass slide placing area at the bottom of the glass slide cleaning pool (1) is provided with a plurality of rows of tombstones (11), confluence channels (12) are arranged beside the tombstones (11), and other areas of the glass slide cleaning pool (1) are slopes inclined to the glass slide placing area.
4. A slide soaking and washing apparatus according to claim 3, wherein: the cleaning liquid outlet of the glass slide cleaning tank (1) is provided with a four-way valve (13), and the liquid outlet of the four-way valve (13) is communicated with the wastewater collection box (4).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711018043.2A CN107677533B (en) | 2017-10-23 | 2017-10-23 | Slide soaking and cleaning device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711018043.2A CN107677533B (en) | 2017-10-23 | 2017-10-23 | Slide soaking and cleaning device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107677533A CN107677533A (en) | 2018-02-09 |
| CN107677533B true CN107677533B (en) | 2024-03-01 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711018043.2A Active CN107677533B (en) | 2017-10-23 | 2017-10-23 | Slide soaking and cleaning device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107677533B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109443888B (en) * | 2018-10-29 | 2020-11-24 | 重庆英特力科技有限公司 | Microbial staining device |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1994023326A1 (en) * | 1993-04-01 | 1994-10-13 | Hybaid Limited | Microscope slide holder |
| CN101219015A (en) * | 2007-01-12 | 2008-07-16 | 上海市闵行第二中学 | Novel height-adjustable table |
| CN201327458Y (en) * | 2008-12-25 | 2009-10-14 | 宁波美生医疗器材有限公司 | Swing liquid device for preparing liquid-based thin-layer cell |
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| CN104949876A (en) * | 2014-01-10 | 2015-09-30 | 南京医科大学第一附属医院 | Detachable, volume-adjustable and space-sealed glass slide incubator reasonable in structure |
| CN205200049U (en) * | 2015-12-15 | 2016-05-04 | 张建 | Glass slide cleaning device for pathology department |
| CN106706398A (en) * | 2017-02-08 | 2017-05-24 | 爱威科技股份有限公司 | Sample staining device |
| CN206248424U (en) * | 2016-12-01 | 2017-06-13 | 辽东学院 | Support adjustable multifunctional dye vat |
| CN206484809U (en) * | 2017-02-09 | 2017-09-12 | 东莞市玛雅精密模具有限公司 | A kind of adjustable charge door of die casting moulding mechanism |
| CN207280834U (en) * | 2017-10-23 | 2018-04-27 | 宁波大学 | Glass slide soaks and cleaning device |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10218988C1 (en) * | 2002-04-24 | 2003-11-20 | Horst Dieter Becker | Device and method for wetting objects |
-
2017
- 2017-10-23 CN CN201711018043.2A patent/CN107677533B/en active Active
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1994023326A1 (en) * | 1993-04-01 | 1994-10-13 | Hybaid Limited | Microscope slide holder |
| CN101219015A (en) * | 2007-01-12 | 2008-07-16 | 上海市闵行第二中学 | Novel height-adjustable table |
| CN201327458Y (en) * | 2008-12-25 | 2009-10-14 | 宁波美生医疗器材有限公司 | Swing liquid device for preparing liquid-based thin-layer cell |
| CN202725986U (en) * | 2012-06-14 | 2013-02-13 | 芜湖咸保机械制造有限公司 | Demoulding device for casting mould |
| CN203578326U (en) * | 2013-10-10 | 2014-05-07 | 江苏协鑫硅材料科技发展有限公司 | Silicon cleaning basket |
| CN104949876A (en) * | 2014-01-10 | 2015-09-30 | 南京医科大学第一附属医院 | Detachable, volume-adjustable and space-sealed glass slide incubator reasonable in structure |
| CN205200049U (en) * | 2015-12-15 | 2016-05-04 | 张建 | Glass slide cleaning device for pathology department |
| CN206248424U (en) * | 2016-12-01 | 2017-06-13 | 辽东学院 | Support adjustable multifunctional dye vat |
| CN106706398A (en) * | 2017-02-08 | 2017-05-24 | 爱威科技股份有限公司 | Sample staining device |
| CN206484809U (en) * | 2017-02-09 | 2017-09-12 | 东莞市玛雅精密模具有限公司 | A kind of adjustable charge door of die casting moulding mechanism |
| CN207280834U (en) * | 2017-10-23 | 2018-04-27 | 宁波大学 | Glass slide soaks and cleaning device |
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| Publication number | Publication date |
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| CN107677533A (en) | 2018-02-09 |
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