CN218653089U - Protein purification chromatographic column equipment - Google Patents
Protein purification chromatographic column equipment Download PDFInfo
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- CN218653089U CN218653089U CN202223121120.6U CN202223121120U CN218653089U CN 218653089 U CN218653089 U CN 218653089U CN 202223121120 U CN202223121120 U CN 202223121120U CN 218653089 U CN218653089 U CN 218653089U
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Abstract
The utility model relates to a gel chromatography equipment technical field's a protein purification chromatography column equipment, including the pole setting support, pole setting support lower extreme fixed mounting bears the dish, bear and set up two trompils based on its diameter symmetric distribution on the dish, and be connected with row material pipe and drain pipe respectively at two trompil lower extremes, bear and set up coaxial rotation on the dish and install the rotary disk, set up two trompils based on its diameter symmetric distribution on the rotary disk, and be connected with the chromatography post respectively on two trompils, pole setting support upper end liftable movable mounting has the mounting panel, mounting panel bottom both sides are provided with can be respectively with two closed rubber plugs of chromatography capital end shutoff, when detecting big batch sample, need not to consume too much latency, also need not loaded down with trivial details artifical cleanness simultaneously, be favorable to optimizing equipment convenience in use and efficiency.
Description
Technical Field
The utility model relates to a protein purification chromatography column equipment belongs to gel chromatography equipment technical field.
Background
Protein isolation and purification is a widely used and important technique in biochemical research applications, and a typical eukaryotic cell may contain thousands of different proteins, some very abundant, some only in a few copies, and in order to study a protein, it is necessary to first purify the protein from other proteins and non-protein molecules.
The chromatographic column is the main part in the gel chromatography technique, according to each component among the sample mixture in the stationary phase and the mobile phase distribution coefficient different, thereby separate, one set of equipment of current gel chromatography equipment only is equipped with a chromatographic column, when carrying out sample separation purification, end present sample chromatography detection back, need wait that the chromatographic column is clean dry in order to avoid both samples to appear a great amount of mutual doping and influence the testing result, consequently when detecting facing batch sample, not only clean instrument complex operation, and clean and wait for dry need occupy a large amount of time, thereby influence detection work efficiency.
SUMMERY OF THE UTILITY MODEL
In order to solve the problems existing in the prior art, the utility model provides a protein purification chromatographic column device.
The technical scheme of the utility model as follows:
a protein purification chromatographic column device comprises a vertical rod support, wherein a bearing disc is fixedly arranged at the lower end of the vertical rod support, two openings which are symmetrically distributed based on the diameter of the bearing disc are formed in the bearing disc, the lower ends of the two openings are respectively connected with a material discharging pipe and a water discharging pipe, a rotating disc is coaxially and rotatably arranged on the bearing disc, two openings which are symmetrically distributed based on the diameter of the rotating disc are formed in the rotating disc, chromatographic columns are respectively connected to the upper ends of the two openings, the lower ends of the two chromatographic columns are respectively communicated with the material discharging pipe and the water discharging pipe, and the two chromatographic columns can be switched after the rotating disc and the bearing disc rotate 180 degrees relatively;
the device comprises a vertical rod support, a mounting plate, two rubber plugs and a water injection pipe, wherein the mounting plate is movably mounted at the upper end of the vertical rod support in a lifting mode, the two sides of the bottom of the mounting plate are provided with the rubber plugs which can be respectively closed with the top ends of two chromatographic columns in a plugging mode, the two rubber plugs are internally provided with the water injection pipe and the water injection pipe in a penetrating mode, and the rubber plugs are separated from the top ends of the chromatographic columns after the mounting plate is lifted.
The upright rod support comprises a base, and a mounting upright column is vertically arranged on the base.
The side wall of the bearing plate is connected with a hoop through a cantilever rod, and the hoop is fixedly installed on the installation upright post.
The mounting plate side wall is connected with a sleeve through a cantilever rod, the sleeve is sleeved on the mounting upright in a sliding mode, the outer wall of the mounting upright is located on the upper side and the lower side of the sleeve, an upper retaining ring and a lower retaining ring are fixedly arranged on the upper side and the lower side of the sleeve respectively, a reset pressure spring is sleeved on the outer wall of the mounting upright, and two ends of the reset pressure spring are abutted to the upper retaining ring and the sleeve.
And sealing gaskets are embedded at the lower end edges of the two chromatographic columns.
Wherein, arrange material pipe, drain pipe, notes material pipe and water injection pipe and all be provided with the ooff valve respectively.
The input end of the water injection pipe is communicated with the water storage tank through a water delivery pipe, and a water delivery pump is arranged on the water delivery pipe.
Wherein, the one end that the water injection pipe inserts the chromatography column is connected with the scattering nozzle.
The utility model discloses following beneficial effect has:
the utility model relates to a protein purification chromatographic column equipment is equipped with two chromatographic columns, can switch over two chromatographic columns after rotating 180 degrees relatively through rotary disk and carrier plate and exchange the position to realize switching between work station and clean station, is equipped with water delivery cleaning system simultaneously, can communicate with the chromatographic column that lies in clean station in it, and during actual work, a chromatographic column is in work station and is used for the chromatography of working, and another chromatographic column is then in clean station and can clean and dry simultaneously; therefore, when detecting a large amount of samples, excessive waiting time does not need to be consumed, and meanwhile, tedious manual cleaning is not needed, so that the convenience and efficiency of using the equipment are favorably optimized.
Drawings
FIG. 1 is a schematic structural view of a protein purification chromatography column apparatus of the present invention;
FIG. 2 is a schematic view of the structure of a carrier tray of the protein purification chromatography column apparatus of the present invention;
fig. 3 is a schematic view of the rotary disk structure of the protein purification chromatography column apparatus of the present invention.
The reference numbers in the figures denote:
1-upright rod support, 101-base, 102-mounting upright column, 2-bearing disc, 3-discharging pipe, 4-water discharging pipe, 5-rotating disc, 6-chromatographic column, 7-mounting plate, 8-rubber plug, 9-material injecting pipe, 10-water injecting pipe, 11-hoop, 12-sleeve, 13-upper baffle ring, 14-lower baffle ring, 15-reset pressure spring, 16-sealing washer, 17-water storage tank, 18-water conveying pump and 19-scattering nozzle.
Detailed Description
The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments.
Referring to fig. 1-3, a protein purification chromatography column apparatus comprises a vertical rod support 1, the vertical rod support 1 comprises a base 101, and a mounting upright column 102 is vertically arranged on the base 101;
the lower end of the upright rod bracket 1 is fixedly provided with a bearing disc 2, specifically, the side wall of the bearing disc 2 is connected with a hoop 11 through a cantilever rod, and the hoop 11 is fixedly arranged on the mounting upright post 102;
the bearing disc 2 is provided with two openings which are symmetrically distributed based on the diameter of the bearing disc, the lower ends of the two openings are respectively connected with a discharging pipe 3 and a drain pipe 4, the discharging pipe 3 and the drain pipe 4 are respectively provided with a switch valve, the bearing disc 2 is coaxially and rotatably provided with a rotating disc 5, the rotating disc 5 is provided with two openings which are symmetrically distributed based on the diameter of the rotating disc 5, the upper ends of the two openings are respectively connected with chromatographic columns 6, the lower end edges of the two chromatographic columns 6 are respectively embedded with a sealing gasket 16, the lower ends of the two chromatographic columns 6 are respectively communicated with the discharging pipe 3 and the drain pipe 4, and the rotating disc 5 and the bearing disc 2 can be switched to the opposite positions after rotating 180 degrees relatively;
the upper end of the vertical rod support 1 is movably provided with a mounting plate 7 in a lifting manner, specifically, the side wall of the mounting plate 7 is connected with a sleeve 12 through a cantilever rod, the sleeve 12 is sleeved on a mounting upright post 102 in a sliding manner, an upper retaining ring 13 and a lower retaining ring 14 are respectively and fixedly arranged on the upper side and the lower side of the sleeve 12 on the outer wall of the mounting upright post 102, a reset pressure spring 15 is sleeved on the outer wall of the mounting upright post 102, and two ends of the reset pressure spring 15 are abutted between the upper retaining ring 13 and the sleeve 12;
rubber plugs 8 which can be respectively plugged and closed with the top ends of the two chromatographic columns 6 are arranged on two sides of the bottom of the mounting plate 7, a material injection pipe 9 and a water injection pipe 10 are respectively arranged in the two rubber plugs 8 in a penetrating manner, the material injection pipe 9 and the water injection pipe 10 are respectively provided with a switch valve, and the rubber plugs 8 are separated from the top ends of the chromatographic columns 6 after the mounting plate 7 is lifted;
the input end of the water injection pipe 10 is communicated with the water storage tank 17 through a water delivery pipe, a water delivery pump 18 is arranged on the water delivery pipe, and one end, connected with the chromatographic column 6, of the water injection pipe 10 is connected with a scattering nozzle 19 to improve the cleaning effect of the inner pipe wall through scattering high-pressure water flow.
The utility model discloses a theory of operation:
lifting the mounting plate 7 to separate the rubber plug 8 from the top end of the chromatographic column 6, simultaneously rotating and adjusting the angle of the rotating disk 5, communicating the lower ends of two chromatographic columns 6 on the rotating disk 5 with the discharging pipe 3 and the drain pipe 4 respectively, lowering the mounting plate 7 to make the rubber plug 8 contact with the top end of the chromatographic column 6 for plugging, opening the switch valve on the feeding pipe 9, injecting the chromatographic treatment material into the chromatographic column 6 on the left working station through the feeding pipe 9, closing the switch valve on the feeding pipe 9 after the feeding is completed, and performing relevant subsequent detection operations such as standing, opening the switch valve on the discharging pipe 3 after the detection is completed, discharging the sample in the chromatographic column 6 on the left working station, closing the switch valve on the discharging pipe 3 after the discharge is completed, then lifting the mounting plate 7 again to separate the rubber plug 8 from the top end of the chromatographic column 6, simultaneously rotating and adjusting the rotating disk 5180, switching the positions of the two chromatographic columns 6, replacing the new chromatographic column 6 on the working station to perform the chromatographic treatment, cleaning, replacing the chromatographic column 6 on the cleaning station to clean, opening the switch valve of the cleaning station, opening the high-pressure water pump 18 to clean the cleaning switch valve, and closing the drain pipe, and cleaning switch valve, and closing the high pressure water pump to clean the sewage after the cleaning station, and cleaning switch 4 to clean the sewage.
The above only is the embodiment of the present invention, not limiting the patent scope of the present invention, all the equivalent structures or equivalent processes that are used in the specification and the attached drawings or directly or indirectly applied to other related technical fields are included in the patent protection scope of the present invention.
Claims (8)
1. The utility model provides a protein purification chromatographic column equipment which characterized in that: the device comprises a vertical rod support (1), wherein a bearing disc (2) is fixedly installed at the lower end of the vertical rod support (1), two open holes based on the diameter symmetric distribution of the open holes are formed in the bearing disc (2), a material discharging pipe (3) and a water discharging pipe (4) are respectively connected to the lower ends of the two open holes, a rotating disc (5) is coaxially installed on the bearing disc (2) in a rotating mode, two open holes based on the diameter symmetric distribution of the open holes are formed in the rotating disc (5), chromatography columns (6) are respectively connected to the upper ends of the two open holes, the lower ends of the two chromatography columns (6) are respectively communicated with the material discharging pipe (3) and the water discharging pipe (4), and the rotating disc (5) and the bearing disc (2) can switch positions after rotating 180 degrees relatively;
liftable movable mounting in pole setting support (1) upper end has mounting panel (7), mounting panel (7) bottom both sides are provided with can be respectively with two chromatography columns (6) top shutoff closed rubber end cap (8), wear to be equipped with in two rubber end caps (8) respectively and annotate material pipe (9) and water injection pipe (10), lift back on mounting panel (7) rubber end cap (8) and chromatography column (6) top separation.
2. The protein purification chromatography column apparatus of claim 1, wherein: the upright rod support (1) comprises a base (101), and a mounting upright post (102) is vertically arranged on the base (101).
3. A protein purification chromatography column apparatus as claimed in claim 2, wherein: the side wall of the bearing plate (2) is connected with a hoop (11) through a cantilever rod, and the hoop (11) is fixedly arranged on the mounting upright post (102).
4. A protein purification chromatography column apparatus as claimed in claim 2, wherein: mounting panel (7) lateral wall is connected with sleeve (12) through the cantilever pole, sleeve (12) slide cup joint in on installation stand (102), installation stand (102) outer wall is located both sides are fixed respectively about sleeve (12) and are provided with and keep off ring (13) and keep off ring (14) down, installation stand (102) outer wall cover is equipped with pressure spring (15) that resets, pressure spring (15) both ends butt in go up and keep off between ring (13) and sleeve (12).
5. The protein purification chromatography column apparatus of claim 1, wherein: and sealing gaskets (16) are embedded at the lower end edges of the two chromatographic columns (6).
6. The protein purification chromatography column apparatus of claim 1, wherein: the discharging pipe (3), the water discharging pipe (4), the material injection pipe (9) and the water injection pipe (10) are respectively provided with a switch valve.
7. The protein purification chromatography column apparatus of claim 1, wherein: the input end of the water injection pipe (10) is communicated with the water storage tank (17) through a water delivery pipe, and a water delivery pump (18) is arranged on the water delivery pipe.
8. The protein purification chromatography column apparatus of claim 1, wherein: one end of the water injection pipe (10) connected to the chromatographic column (6) is connected with a scattering nozzle (19).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223121120.6U CN218653089U (en) | 2022-11-23 | 2022-11-23 | Protein purification chromatographic column equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223121120.6U CN218653089U (en) | 2022-11-23 | 2022-11-23 | Protein purification chromatographic column equipment |
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| Publication Number | Publication Date |
|---|---|
| CN218653089U true CN218653089U (en) | 2023-03-21 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202223121120.6U Active CN218653089U (en) | 2022-11-23 | 2022-11-23 | Protein purification chromatographic column equipment |
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| CN (1) | CN218653089U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115999198A (en) * | 2023-03-27 | 2023-04-25 | 石药集团百克(山东)生物制药股份有限公司 | Column chromatography equipment |
-
2022
- 2022-11-23 CN CN202223121120.6U patent/CN218653089U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115999198A (en) * | 2023-03-27 | 2023-04-25 | 石药集团百克(山东)生物制药股份有限公司 | Column chromatography equipment |
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