CN107677533A - Slide soaks and cleaning device - Google Patents
Slide soaks and cleaning device Download PDFInfo
- Publication number
- CN107677533A CN107677533A CN201711018043.2A CN201711018043A CN107677533A CN 107677533 A CN107677533 A CN 107677533A CN 201711018043 A CN201711018043 A CN 201711018043A CN 107677533 A CN107677533 A CN 107677533A
- Authority
- CN
- China
- Prior art keywords
- slide
- immersion
- frame
- service sink
- cleaning device
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000004140 cleaning Methods 0.000 title claims abstract description 25
- 238000007654 immersion Methods 0.000 claims abstract description 51
- 230000003028 elevating effect Effects 0.000 claims abstract description 11
- 238000012360 testing method Methods 0.000 claims abstract description 6
- 239000007788 liquid Substances 0.000 claims description 10
- 238000005452 bending Methods 0.000 claims description 3
- 239000011521 glass Substances 0.000 claims description 3
- 239000010865 sewage Substances 0.000 claims description 2
- 238000013467 fragmentation Methods 0.000 abstract description 11
- 238000006062 fragmentation reaction Methods 0.000 abstract description 11
- 239000012530 fluid Substances 0.000 abstract description 5
- 238000000034 method Methods 0.000 description 6
- 244000005700 microbiome Species 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 4
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 4
- 239000010931 gold Substances 0.000 description 4
- 229910052737 gold Inorganic materials 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 3
- 230000034994 death Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 230000015556 catabolic process Effects 0.000 description 2
- 230000030833 cell death Effects 0.000 description 2
- 238000000354 decomposition reaction Methods 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 238000011065 in-situ storage Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 108010077544 Chromatin Proteins 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 241000275031 Nica Species 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 208000026935 allergic disease Diseases 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000006907 apoptotic process Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 210000003483 chromatin Anatomy 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 125000001475 halogen functional group Chemical group 0.000 description 1
- 230000009610 hypersensitivity Effects 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/04—Cleaning involving contact with liquid
- B08B3/048—Overflow-type cleaning, e.g. tanks in which the liquid flows over the tank in which the articles are placed
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a kind of immersion of slide and cleaning device, including slide service sink and slide immersion folder, the angle adjusting piece of slide immersion folder is connected with the lowering or hoisting gear on slide service sink, and advantage is that slide is clamped by slide immersion folder and difficultly slipped off;The inclined angle of slide can be become more meticulous stability contorting by angle adjusting piece, enable the removal that solution is light and slow, in order to avoid fragmentation DNA drifts about, error is caused to experimental result, while be soaked and cleaning fluid dosage is reduced and is easily recycled after testing;The elevating lever and support member that lowering or hoisting gear includes rotate gear, engaged with rotate gear, the drive shaft of rotate gear is arranged on by bearing on the outer wall of slide service sink, the guide pad for being passed through for elevating lever is fixed with the outer wall of slide service sink, angle adjusting piece axle is connected on support member, allow the present apparatus by slide is hanging or patch bottom so that each step of experimentation can be operated on demand.
Description
Technical field
The present invention relates to laboratory services technical field, more particularly, to a kind of immersion of slide and cleaning device.
Background technology
The cell death of bacterium is probably the process of sequencing, similar to the apoptosis phenomenon in higher organism body, such as through
The death process of bacterium after bactericidal antibiotic effect.Paper《Jos é Luis Fern á ndez, M ó nica Cartelle,
Lourdes Muriel, et al.DNA Fragmentation in Microorganisms Assessed In Situ [J]
.Applied and environmental microbiology, 2008,74 (19):5925-5933.》And Publication No.
CN101589156A Chinese invention patent《Method for determining DNA fragmentation in microorganisms》Describe and be used for on-spot study
The method of the chromatin of microorganism/DNA integralities evaluates the death of bacterium, and this method is paid special attention to is caused by DNA degradation
Dead or death caused by DNA degradation.Cell in the inertia Ago-Gel being immersed on slide glass is cracked:If
Cell has the DNA of fragmentation, then fragment can be diffused in agarose medium from original core, it will thus be seen that arriving DNA pieces
Wide halo caused by section spreads to surrounding;Use hypersensitivity fluorescent dye, such as SYBRGold (SYBR gold coloring agent), energy
The nucleoid DNA fiber of microorganism is enough distinguished, particularly can accurately be shown in this DNA fragmentation event and be flooded to agar
Small DNA fragmentation in sugar.So just be possible to obtain a kind of relative ease and quick instrument, it can be in a repeatable fashion
DNA break of the cell ground in in-situ evaluation bacterium and other microorganisms one by one;, can be by microscope after being acted on through decomposition agent
Level observes the DNA molecular of fragmentation and quickly analyzes bacterial cell death.
Slide is soaked in above-mentioned experimentation and cleans the horizontal level operation that should try one's best, removes solution on slide
Operation it is light and slow, in order to avoid fragmentation DNA drift about, error is caused to experimental result.And under existing experiment condition, slide
Immersion and cleaning need to be gripped with tweezers, slide be present and easily slide, it is impossible to the shortcomings that carrying out refinement, cause to test
As a result undesirable and experiment reagent waste.Therefore it is badly in need of a kind of experiment behaviour of method for DNA fragmentation in microorganisms
Make device, the immersion and cleaning of the operation slide that can more become more meticulous.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of slide immersion and cleaning device, can more become more meticulous
Operation slide immersion and cleaning.
Technical scheme is used by the present invention solves above-mentioned technical problem:A kind of slide immersion and cleaning device, bag
Slide service sink and slide immersion folder are included, the angle adjusting piece of the slide immersion folder is with being arranged on slide service sink
On lowering or hoisting gear connection.
The lowering or hoisting gear includes rotate gear, the elevating lever engaged with rotate gear and the branch for being fixed on elevating lever top
Support member;The drive shaft of the rotate gear is arranged on by bearing on the outer wall of slide service sink, the slide service sink
Outer wall on be fixed with guide pad for passing through for elevating lever;The angle adjusting piece axle is connected on support member.Make the present apparatus
Can slide is hanging or patch bottom so that each step of experimentation can be operated on demand.
Compared with prior art, the advantage of the invention is that slide is clamped and difficultly slipped off by slide immersion folder;Carry
The inclined angle of slide can be become more meticulous stability contorting by angle adjusting piece, enable the removal that solution is light and slow, in order to avoid fragment
Change DNA drifts, error is caused to experimental result, meanwhile, immersion and cleaning fluid dosage are reduced and are easily recycled after testing, so as to
Reach the operation more to become more meticulous.
Keep flat frame as an improvement, the slide immersion folder includes slide and keep flat the be hinged intermediate plate of frame with slide,
The intermediate plate and slide, which are kept flat, is provided with torsion spring between frame, and slide, can be by very flat no matter in horizontal or obliquity
Steady clamps;The angle adjusting piece by bending connecting portion and adjustment portion and be fixed on adjustment portion free end swingle connect
Composition is connect, adjustment portion and the support member axle of the angle adjusting piece connect, and the bottom of the connecting portion of the angle adjusting piece is with carrying glass
Piece keeps flat frame and is fixedly connected, and the angle of inclination of slide can be and more steady than manual operations by accurate operation, makes solution
Removal that can be light and slow.
As an improvement, the intermediate plate head is hinged with immersion frame;The immersion frame frame bottom, which is provided with, to be used to supply slide
Keep flat the groove of frame test block frame upper protruding block insertion.Immersion frame make it that the fixation of slide is more firm, and projection makes load
Solution above slide will not overflow, and reduce the superjacent air space of slide, so as to reduce the use of soak and cleaning fluid
Amount.
As an improvement, the intermediate plate head is hinged with the cover plate that is used to cover immersion frame coaxial with immersion frame.With
When SYBR Gold dye to DNA fragmentation, SYBR Gold are clear to light decomposition, and dark environment is beneficial to DNA and SYBR Gold
Combination;And when cover plate progress slight wobble is closed when solution is unbalanced, solution is not easy to overflow.
As an improvement, the immersion frame frame is provided with soak and collects ball, soak is set to collect the immersion frame side of ball
Inframe side is provided with poly- liquid block, and the through hole that the immersion frame frame connection soak collects ball is provided with switch valve, the soak
Hole is drawn provided with pipettor in the top for collecting ball.When removing solution above slide, easy centralized collection and removal.
As an improvement, the slide lay down area of the bottom of the slide service sink is provided with multiple rows of Ling Tai, it is provided with by the platform of mound
Busway, other regions of the slide service sink are to the inclined slope of slide lay down area.Lay down area is provided with Ling Tai,
The contact area of slide and bottom can be reduced, makes the solution below slide be easier to flow, so as to lift the effect of cleaning
Fruit, and can strengthen the volatilization of moisture below slide in slide baking stage;And it is provided with busway by the platform of mound so that useless
Liquid when slide horizontal level is placed just can centralized collection, reduce the inclination of slide, in order to avoid fragmentation DNA drift,
Influence experimental result;Flowed through during the solution backflow for setting surrounding slope to be overflowed when causing cleaning above slide, so as to subtract
The dosage of few cleaning fluid.
As an improvement, the wash liquid stream source of the slide service sink is provided with four-way valve, the liquid outlet of four-way valve is with giving up
Water collection tank connects.So that soak, cleaning fluid are easier centralized collection and removal.
Brief description of the drawings
Fig. 1 is the positive structure diagram of the present invention.
Fig. 2 is the schematic top plan view of slide service sink of the present invention.
Fig. 3 is the schematic top plan view of present invention immersion frame.
Fig. 4 is Fig. 3 A-A sectional views.
Embodiment
The present invention is described in further detail below in conjunction with accompanying drawing embodiment.
This preferred embodiment is as shown in Figures 1 to 4 a kind of immersion of slide and cleaning device, including slide service sink 1
Soak and press from both sides with slide, the angle adjusting piece 24 of slide immersion folder connects with the lowering or hoisting gear on slide service sink 1
Connect.
Lowering or hoisting gear includes rotate gear 31, the elevating lever 32 engaged with rotate gear 31 and is fixed on the top of elevating lever 32
Support member 33;The drive shaft 35 of rotate gear 31 is arranged on by bearing 37 on the outer wall of slide service sink 1, and slide is clear
The guide pad 34 for being passed through for elevating lever 32 is fixed with the outer wall of wash pool 1;The axle of angle adjusting piece 21 is connected on support member 33.
Slide immersion folder includes slide and keeps flat frame 21 and keep flat the be hinged intermediate plate 22 of frame 21 with slide, intermediate plate 22 and
Slide is provided with torsion spring 23 between keeping flat frame 21;Angle adjusting piece 24 by bending connecting portion and adjustment portion and be fixed on adjustment portion
The swingle connection composition of free end, adjustment portion and the axle of support member 33 of angle adjusting piece 24 connect, the connection of angle adjusting piece 24
The bottom in portion keeps flat frame 21 with slide and is fixedly connected.
The head of intermediate plate 22 is hinged with immersion frame 25;Immersion frame 25 frame bottom is provided with tests for keeping flat frame 21 for slide
The groove 251 that area's frame upper protruding block 211 is embedded in.
The head of intermediate plate 22 is hinged with the cover plate 26 that is used to cover immersion frame 25 coaxial with immersion frame 25.
Soak the frame of frame 25 and be provided with soak collection ball 27, set soak to collect the scuncheon of immersion frame 25 of ball 27
Provided with poly- liquid block 251, the through hole 252 that immersion frame 25 frame connection soak collects ball 27 is provided with switch valve 28, and soak is received
Hole 271 is drawn provided with pipettor in the top of collection ball 27.
The slide lay down area of the bottom of slide service sink 1 is provided with multiple rows of mound platform 11, and the side of mound platform 11 is provided with busway
12, other regions of slide service sink 1 are to the inclined slope of slide lay down area.
The wash liquid stream source of slide service sink 1 is provided with four-way valve 13, liquid outlet and the sewage collecting box 4 of four-way valve 13
Connection.
Claims (8)
1. a kind of slide immersion and cleaning device, it is characterised in that:Pressed from both sides including slide service sink (1) and slide immersion,
The angle adjusting piece (24) of the slide immersion folder is connected with the lowering or hoisting gear on slide service sink (1).
2. slide immersion according to claim 1 and cleaning device, it is characterised in that:The lowering or hoisting gear includes rotating
Gear (31), the elevating lever (32) engaged with rotate gear (31) and the support member (33) for being fixed on elevating lever (32) top;Institute
The drive shaft (35) for stating rotate gear (31) is arranged on by bearing (37) on the outer wall of slide service sink (1), the load glass
The guide pad (34) for being passed through for elevating lever (32) is fixed with the outer wall of piece service sink (1);The angle adjusting piece (21)
Axle is connected on support member (33).
3. slide immersion according to claim 1 and cleaning device, it is characterised in that:The slide immersion folder includes
Slide keeps flat frame (21) and keeps flat the be hinged intermediate plate (22) of frame (21) with slide, and the intermediate plate (22) keeps flat frame with slide
(21) torsion spring (23) is provided between;The angle adjusting piece (24) by bending connecting portion and adjustment portion and be fixed on adjustment portion from
It is made up of the swingle connection held, adjustment portion and support member (33) axle of the angle adjusting piece (24) connect, the angular adjustment
The bottom of the connecting portion of part (24) keeps flat frame (21) with slide and is fixedly connected.
4. slide immersion according to claim 3 and cleaning device, it is characterised in that:Intermediate plate (22) head is hinged
There is immersion frame (25);Described immersion frame (25) frame bottom, which is provided with, to be used to keep flat frame (21) test block frame upper protruding block for slide
(211) embedded groove (251).
5. slide immersion according to claim 4 and cleaning device, it is characterised in that:Intermediate plate (22) head is hinged
There is the cover plate (26) that is used to cover immersion frame (25) coaxial with immersion frame (25).
6. slide immersion according to claim 4 and cleaning device, it is characterised in that:On described immersion frame (25) frame
Ball (27) is collected provided with soak, sets soak to collect immersion frame (25) scuncheon of ball (27) and is provided with poly- liquid block (251),
The through hole (252) that immersion frame (25) frame connection soak collects ball (27) is provided with switch valve (28), the soak
Hole (271) is drawn provided with pipettor in the top for collecting ball (27).
7. slide immersion according to claim 1 and cleaning device, it is characterised in that:The slide service sink (1)
The slide lay down area of bottom be provided with multiple rows of Ling Tai (11), is provided with busway (12) by Ling Tai (11), the slide is clear
Other regions of wash pool (1) are to the inclined slope of slide lay down area.
8. slide immersion according to claim 7 and cleaning device, it is characterised in that:The slide service sink (1)
Wash liquid stream source be provided with four-way valve (13), the liquid outlet of four-way valve (13) connects with sewage collecting box (4).
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201711018043.2A CN107677533B (en) | 2017-10-23 | 2017-10-23 | Slide soaking and cleaning device |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201711018043.2A CN107677533B (en) | 2017-10-23 | 2017-10-23 | Slide soaking and cleaning device |
Publications (2)
Publication Number | Publication Date |
---|---|
CN107677533A true CN107677533A (en) | 2018-02-09 |
CN107677533B CN107677533B (en) | 2024-03-01 |
Family
ID=61142779
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201711018043.2A Active CN107677533B (en) | 2017-10-23 | 2017-10-23 | Slide soaking and cleaning device |
Country Status (1)
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CN (1) | CN107677533B (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109443888A (en) * | 2018-10-29 | 2019-03-08 | 重庆英特力科技有限公司 | A kind of microbial staining device |
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Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1994023326A1 (en) * | 1993-04-01 | 1994-10-13 | Hybaid Limited | Microscope slide holder |
US20060239858A1 (en) * | 2002-04-24 | 2006-10-26 | Becker Horst D | Device and method for wetting objects |
CN101219015A (en) * | 2007-01-12 | 2008-07-16 | 上海市闵行第二中学 | Novel height-adjustable table |
CN201327458Y (en) * | 2008-12-25 | 2009-10-14 | 宁波美生医疗器材有限公司 | Swing liquid device for preparing liquid-based thin-layer cell |
CN202725986U (en) * | 2012-06-14 | 2013-02-13 | 芜湖咸保机械制造有限公司 | Demoulding device for casting mould |
CN203578326U (en) * | 2013-10-10 | 2014-05-07 | 江苏协鑫硅材料科技发展有限公司 | Silicon cleaning basket |
CN104949876A (en) * | 2014-01-10 | 2015-09-30 | 南京医科大学第一附属医院 | Detachable, volume-adjustable and space-sealed glass slide incubator reasonable in structure |
CN205200049U (en) * | 2015-12-15 | 2016-05-04 | 张建 | Glass slide cleaning device for pathology department |
CN106706398A (en) * | 2017-02-08 | 2017-05-24 | 爱威科技股份有限公司 | Sample staining device |
CN206248424U (en) * | 2016-12-01 | 2017-06-13 | 辽东学院 | Support adjustable multifunctional dye vat |
CN206484809U (en) * | 2017-02-09 | 2017-09-12 | 东莞市玛雅精密模具有限公司 | A kind of adjustable charge door of die casting moulding mechanism |
CN207280834U (en) * | 2017-10-23 | 2018-04-27 | 宁波大学 | Glass slide soaks and cleaning device |
-
2017
- 2017-10-23 CN CN201711018043.2A patent/CN107677533B/en active Active
Patent Citations (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1994023326A1 (en) * | 1993-04-01 | 1994-10-13 | Hybaid Limited | Microscope slide holder |
US20060239858A1 (en) * | 2002-04-24 | 2006-10-26 | Becker Horst D | Device and method for wetting objects |
CN101219015A (en) * | 2007-01-12 | 2008-07-16 | 上海市闵行第二中学 | Novel height-adjustable table |
CN201327458Y (en) * | 2008-12-25 | 2009-10-14 | 宁波美生医疗器材有限公司 | Swing liquid device for preparing liquid-based thin-layer cell |
CN202725986U (en) * | 2012-06-14 | 2013-02-13 | 芜湖咸保机械制造有限公司 | Demoulding device for casting mould |
CN203578326U (en) * | 2013-10-10 | 2014-05-07 | 江苏协鑫硅材料科技发展有限公司 | Silicon cleaning basket |
CN104949876A (en) * | 2014-01-10 | 2015-09-30 | 南京医科大学第一附属医院 | Detachable, volume-adjustable and space-sealed glass slide incubator reasonable in structure |
CN205200049U (en) * | 2015-12-15 | 2016-05-04 | 张建 | Glass slide cleaning device for pathology department |
CN206248424U (en) * | 2016-12-01 | 2017-06-13 | 辽东学院 | Support adjustable multifunctional dye vat |
CN106706398A (en) * | 2017-02-08 | 2017-05-24 | 爱威科技股份有限公司 | Sample staining device |
CN206484809U (en) * | 2017-02-09 | 2017-09-12 | 东莞市玛雅精密模具有限公司 | A kind of adjustable charge door of die casting moulding mechanism |
CN207280834U (en) * | 2017-10-23 | 2018-04-27 | 宁波大学 | Glass slide soaks and cleaning device |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN109443888A (en) * | 2018-10-29 | 2019-03-08 | 重庆英特力科技有限公司 | A kind of microbial staining device |
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