CN107677533A - Slide soaks and cleaning device - Google Patents

Slide soaks and cleaning device Download PDF

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Publication number
CN107677533A
CN107677533A CN201711018043.2A CN201711018043A CN107677533A CN 107677533 A CN107677533 A CN 107677533A CN 201711018043 A CN201711018043 A CN 201711018043A CN 107677533 A CN107677533 A CN 107677533A
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CN
China
Prior art keywords
slide
immersion
frame
service sink
cleaning device
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201711018043.2A
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Chinese (zh)
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CN107677533B (en
Inventor
沈锡权
陈俊粤
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Ningbo University
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Ningbo University
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Priority to CN201711018043.2A priority Critical patent/CN107677533B/en
Publication of CN107677533A publication Critical patent/CN107677533A/en
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31Apparatus therefor
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B08CLEANING
    • B08BCLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B3/00Cleaning by methods involving the use or presence of liquid or steam
    • B08B3/04Cleaning involving contact with liquid
    • B08B3/048Overflow-type cleaning, e.g. tanks in which the liquid flows over the tank in which the articles are placed

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention discloses a kind of immersion of slide and cleaning device, including slide service sink and slide immersion folder, the angle adjusting piece of slide immersion folder is connected with the lowering or hoisting gear on slide service sink, and advantage is that slide is clamped by slide immersion folder and difficultly slipped off;The inclined angle of slide can be become more meticulous stability contorting by angle adjusting piece, enable the removal that solution is light and slow, in order to avoid fragmentation DNA drifts about, error is caused to experimental result, while be soaked and cleaning fluid dosage is reduced and is easily recycled after testing;The elevating lever and support member that lowering or hoisting gear includes rotate gear, engaged with rotate gear, the drive shaft of rotate gear is arranged on by bearing on the outer wall of slide service sink, the guide pad for being passed through for elevating lever is fixed with the outer wall of slide service sink, angle adjusting piece axle is connected on support member, allow the present apparatus by slide is hanging or patch bottom so that each step of experimentation can be operated on demand.

Description

Slide soaks and cleaning device
Technical field
The present invention relates to laboratory services technical field, more particularly, to a kind of immersion of slide and cleaning device.
Background technology
The cell death of bacterium is probably the process of sequencing, similar to the apoptosis phenomenon in higher organism body, such as through The death process of bacterium after bactericidal antibiotic effect.Paper《Jos é Luis Fern á ndez, M ó nica Cartelle, Lourdes Muriel, et al.DNA Fragmentation in Microorganisms Assessed In Situ [J] .Applied and environmental microbiology, 2008,74 (19):5925-5933.》And Publication No. CN101589156A Chinese invention patent《Method for determining DNA fragmentation in microorganisms》Describe and be used for on-spot study The method of the chromatin of microorganism/DNA integralities evaluates the death of bacterium, and this method is paid special attention to is caused by DNA degradation Dead or death caused by DNA degradation.Cell in the inertia Ago-Gel being immersed on slide glass is cracked:If Cell has the DNA of fragmentation, then fragment can be diffused in agarose medium from original core, it will thus be seen that arriving DNA pieces Wide halo caused by section spreads to surrounding;Use hypersensitivity fluorescent dye, such as SYBRGold (SYBR gold coloring agent), energy The nucleoid DNA fiber of microorganism is enough distinguished, particularly can accurately be shown in this DNA fragmentation event and be flooded to agar Small DNA fragmentation in sugar.So just be possible to obtain a kind of relative ease and quick instrument, it can be in a repeatable fashion DNA break of the cell ground in in-situ evaluation bacterium and other microorganisms one by one;, can be by microscope after being acted on through decomposition agent Level observes the DNA molecular of fragmentation and quickly analyzes bacterial cell death.
Slide is soaked in above-mentioned experimentation and cleans the horizontal level operation that should try one's best, removes solution on slide Operation it is light and slow, in order to avoid fragmentation DNA drift about, error is caused to experimental result.And under existing experiment condition, slide Immersion and cleaning need to be gripped with tweezers, slide be present and easily slide, it is impossible to the shortcomings that carrying out refinement, cause to test As a result undesirable and experiment reagent waste.Therefore it is badly in need of a kind of experiment behaviour of method for DNA fragmentation in microorganisms Make device, the immersion and cleaning of the operation slide that can more become more meticulous.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of slide immersion and cleaning device, can more become more meticulous Operation slide immersion and cleaning.
Technical scheme is used by the present invention solves above-mentioned technical problem:A kind of slide immersion and cleaning device, bag Slide service sink and slide immersion folder are included, the angle adjusting piece of the slide immersion folder is with being arranged on slide service sink On lowering or hoisting gear connection.
The lowering or hoisting gear includes rotate gear, the elevating lever engaged with rotate gear and the branch for being fixed on elevating lever top Support member;The drive shaft of the rotate gear is arranged on by bearing on the outer wall of slide service sink, the slide service sink Outer wall on be fixed with guide pad for passing through for elevating lever;The angle adjusting piece axle is connected on support member.Make the present apparatus Can slide is hanging or patch bottom so that each step of experimentation can be operated on demand.
Compared with prior art, the advantage of the invention is that slide is clamped and difficultly slipped off by slide immersion folder;Carry The inclined angle of slide can be become more meticulous stability contorting by angle adjusting piece, enable the removal that solution is light and slow, in order to avoid fragment Change DNA drifts, error is caused to experimental result, meanwhile, immersion and cleaning fluid dosage are reduced and are easily recycled after testing, so as to Reach the operation more to become more meticulous.
Keep flat frame as an improvement, the slide immersion folder includes slide and keep flat the be hinged intermediate plate of frame with slide, The intermediate plate and slide, which are kept flat, is provided with torsion spring between frame, and slide, can be by very flat no matter in horizontal or obliquity Steady clamps;The angle adjusting piece by bending connecting portion and adjustment portion and be fixed on adjustment portion free end swingle connect Composition is connect, adjustment portion and the support member axle of the angle adjusting piece connect, and the bottom of the connecting portion of the angle adjusting piece is with carrying glass Piece keeps flat frame and is fixedly connected, and the angle of inclination of slide can be and more steady than manual operations by accurate operation, makes solution Removal that can be light and slow.
As an improvement, the intermediate plate head is hinged with immersion frame;The immersion frame frame bottom, which is provided with, to be used to supply slide Keep flat the groove of frame test block frame upper protruding block insertion.Immersion frame make it that the fixation of slide is more firm, and projection makes load Solution above slide will not overflow, and reduce the superjacent air space of slide, so as to reduce the use of soak and cleaning fluid Amount.
As an improvement, the intermediate plate head is hinged with the cover plate that is used to cover immersion frame coaxial with immersion frame.With When SYBR Gold dye to DNA fragmentation, SYBR Gold are clear to light decomposition, and dark environment is beneficial to DNA and SYBR Gold Combination;And when cover plate progress slight wobble is closed when solution is unbalanced, solution is not easy to overflow.
As an improvement, the immersion frame frame is provided with soak and collects ball, soak is set to collect the immersion frame side of ball Inframe side is provided with poly- liquid block, and the through hole that the immersion frame frame connection soak collects ball is provided with switch valve, the soak Hole is drawn provided with pipettor in the top for collecting ball.When removing solution above slide, easy centralized collection and removal.
As an improvement, the slide lay down area of the bottom of the slide service sink is provided with multiple rows of Ling Tai, it is provided with by the platform of mound Busway, other regions of the slide service sink are to the inclined slope of slide lay down area.Lay down area is provided with Ling Tai, The contact area of slide and bottom can be reduced, makes the solution below slide be easier to flow, so as to lift the effect of cleaning Fruit, and can strengthen the volatilization of moisture below slide in slide baking stage;And it is provided with busway by the platform of mound so that useless Liquid when slide horizontal level is placed just can centralized collection, reduce the inclination of slide, in order to avoid fragmentation DNA drift, Influence experimental result;Flowed through during the solution backflow for setting surrounding slope to be overflowed when causing cleaning above slide, so as to subtract The dosage of few cleaning fluid.
As an improvement, the wash liquid stream source of the slide service sink is provided with four-way valve, the liquid outlet of four-way valve is with giving up Water collection tank connects.So that soak, cleaning fluid are easier centralized collection and removal.
Brief description of the drawings
Fig. 1 is the positive structure diagram of the present invention.
Fig. 2 is the schematic top plan view of slide service sink of the present invention.
Fig. 3 is the schematic top plan view of present invention immersion frame.
Fig. 4 is Fig. 3 A-A sectional views.
Embodiment
The present invention is described in further detail below in conjunction with accompanying drawing embodiment.
This preferred embodiment is as shown in Figures 1 to 4 a kind of immersion of slide and cleaning device, including slide service sink 1 Soak and press from both sides with slide, the angle adjusting piece 24 of slide immersion folder connects with the lowering or hoisting gear on slide service sink 1 Connect.
Lowering or hoisting gear includes rotate gear 31, the elevating lever 32 engaged with rotate gear 31 and is fixed on the top of elevating lever 32 Support member 33;The drive shaft 35 of rotate gear 31 is arranged on by bearing 37 on the outer wall of slide service sink 1, and slide is clear The guide pad 34 for being passed through for elevating lever 32 is fixed with the outer wall of wash pool 1;The axle of angle adjusting piece 21 is connected on support member 33.
Slide immersion folder includes slide and keeps flat frame 21 and keep flat the be hinged intermediate plate 22 of frame 21 with slide, intermediate plate 22 and Slide is provided with torsion spring 23 between keeping flat frame 21;Angle adjusting piece 24 by bending connecting portion and adjustment portion and be fixed on adjustment portion The swingle connection composition of free end, adjustment portion and the axle of support member 33 of angle adjusting piece 24 connect, the connection of angle adjusting piece 24 The bottom in portion keeps flat frame 21 with slide and is fixedly connected.
The head of intermediate plate 22 is hinged with immersion frame 25;Immersion frame 25 frame bottom is provided with tests for keeping flat frame 21 for slide The groove 251 that area's frame upper protruding block 211 is embedded in.
The head of intermediate plate 22 is hinged with the cover plate 26 that is used to cover immersion frame 25 coaxial with immersion frame 25.
Soak the frame of frame 25 and be provided with soak collection ball 27, set soak to collect the scuncheon of immersion frame 25 of ball 27 Provided with poly- liquid block 251, the through hole 252 that immersion frame 25 frame connection soak collects ball 27 is provided with switch valve 28, and soak is received Hole 271 is drawn provided with pipettor in the top of collection ball 27.
The slide lay down area of the bottom of slide service sink 1 is provided with multiple rows of mound platform 11, and the side of mound platform 11 is provided with busway 12, other regions of slide service sink 1 are to the inclined slope of slide lay down area.
The wash liquid stream source of slide service sink 1 is provided with four-way valve 13, liquid outlet and the sewage collecting box 4 of four-way valve 13 Connection.

Claims (8)

1. a kind of slide immersion and cleaning device, it is characterised in that:Pressed from both sides including slide service sink (1) and slide immersion, The angle adjusting piece (24) of the slide immersion folder is connected with the lowering or hoisting gear on slide service sink (1).
2. slide immersion according to claim 1 and cleaning device, it is characterised in that:The lowering or hoisting gear includes rotating Gear (31), the elevating lever (32) engaged with rotate gear (31) and the support member (33) for being fixed on elevating lever (32) top;Institute The drive shaft (35) for stating rotate gear (31) is arranged on by bearing (37) on the outer wall of slide service sink (1), the load glass The guide pad (34) for being passed through for elevating lever (32) is fixed with the outer wall of piece service sink (1);The angle adjusting piece (21) Axle is connected on support member (33).
3. slide immersion according to claim 1 and cleaning device, it is characterised in that:The slide immersion folder includes Slide keeps flat frame (21) and keeps flat the be hinged intermediate plate (22) of frame (21) with slide, and the intermediate plate (22) keeps flat frame with slide (21) torsion spring (23) is provided between;The angle adjusting piece (24) by bending connecting portion and adjustment portion and be fixed on adjustment portion from It is made up of the swingle connection held, adjustment portion and support member (33) axle of the angle adjusting piece (24) connect, the angular adjustment The bottom of the connecting portion of part (24) keeps flat frame (21) with slide and is fixedly connected.
4. slide immersion according to claim 3 and cleaning device, it is characterised in that:Intermediate plate (22) head is hinged There is immersion frame (25);Described immersion frame (25) frame bottom, which is provided with, to be used to keep flat frame (21) test block frame upper protruding block for slide (211) embedded groove (251).
5. slide immersion according to claim 4 and cleaning device, it is characterised in that:Intermediate plate (22) head is hinged There is the cover plate (26) that is used to cover immersion frame (25) coaxial with immersion frame (25).
6. slide immersion according to claim 4 and cleaning device, it is characterised in that:On described immersion frame (25) frame Ball (27) is collected provided with soak, sets soak to collect immersion frame (25) scuncheon of ball (27) and is provided with poly- liquid block (251), The through hole (252) that immersion frame (25) frame connection soak collects ball (27) is provided with switch valve (28), the soak Hole (271) is drawn provided with pipettor in the top for collecting ball (27).
7. slide immersion according to claim 1 and cleaning device, it is characterised in that:The slide service sink (1) The slide lay down area of bottom be provided with multiple rows of Ling Tai (11), is provided with busway (12) by Ling Tai (11), the slide is clear Other regions of wash pool (1) are to the inclined slope of slide lay down area.
8. slide immersion according to claim 7 and cleaning device, it is characterised in that:The slide service sink (1) Wash liquid stream source be provided with four-way valve (13), the liquid outlet of four-way valve (13) connects with sewage collecting box (4).
CN201711018043.2A 2017-10-23 2017-10-23 Slide soaking and cleaning device Active CN107677533B (en)

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Application Number Priority Date Filing Date Title
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CN107677533B CN107677533B (en) 2024-03-01

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109443888A (en) * 2018-10-29 2019-03-08 重庆英特力科技有限公司 A kind of microbial staining device

Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994023326A1 (en) * 1993-04-01 1994-10-13 Hybaid Limited Microscope slide holder
US20060239858A1 (en) * 2002-04-24 2006-10-26 Becker Horst D Device and method for wetting objects
CN101219015A (en) * 2007-01-12 2008-07-16 上海市闵行第二中学 Novel height-adjustable table
CN201327458Y (en) * 2008-12-25 2009-10-14 宁波美生医疗器材有限公司 Swing liquid device for preparing liquid-based thin-layer cell
CN202725986U (en) * 2012-06-14 2013-02-13 芜湖咸保机械制造有限公司 Demoulding device for casting mould
CN203578326U (en) * 2013-10-10 2014-05-07 江苏协鑫硅材料科技发展有限公司 Silicon cleaning basket
CN104949876A (en) * 2014-01-10 2015-09-30 南京医科大学第一附属医院 Detachable, volume-adjustable and space-sealed glass slide incubator reasonable in structure
CN205200049U (en) * 2015-12-15 2016-05-04 张建 Glass slide cleaning device for pathology department
CN106706398A (en) * 2017-02-08 2017-05-24 爱威科技股份有限公司 Sample staining device
CN206248424U (en) * 2016-12-01 2017-06-13 辽东学院 Support adjustable multifunctional dye vat
CN206484809U (en) * 2017-02-09 2017-09-12 东莞市玛雅精密模具有限公司 A kind of adjustable charge door of die casting moulding mechanism
CN207280834U (en) * 2017-10-23 2018-04-27 宁波大学 Glass slide soaks and cleaning device

Patent Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994023326A1 (en) * 1993-04-01 1994-10-13 Hybaid Limited Microscope slide holder
US20060239858A1 (en) * 2002-04-24 2006-10-26 Becker Horst D Device and method for wetting objects
CN101219015A (en) * 2007-01-12 2008-07-16 上海市闵行第二中学 Novel height-adjustable table
CN201327458Y (en) * 2008-12-25 2009-10-14 宁波美生医疗器材有限公司 Swing liquid device for preparing liquid-based thin-layer cell
CN202725986U (en) * 2012-06-14 2013-02-13 芜湖咸保机械制造有限公司 Demoulding device for casting mould
CN203578326U (en) * 2013-10-10 2014-05-07 江苏协鑫硅材料科技发展有限公司 Silicon cleaning basket
CN104949876A (en) * 2014-01-10 2015-09-30 南京医科大学第一附属医院 Detachable, volume-adjustable and space-sealed glass slide incubator reasonable in structure
CN205200049U (en) * 2015-12-15 2016-05-04 张建 Glass slide cleaning device for pathology department
CN206248424U (en) * 2016-12-01 2017-06-13 辽东学院 Support adjustable multifunctional dye vat
CN106706398A (en) * 2017-02-08 2017-05-24 爱威科技股份有限公司 Sample staining device
CN206484809U (en) * 2017-02-09 2017-09-12 东莞市玛雅精密模具有限公司 A kind of adjustable charge door of die casting moulding mechanism
CN207280834U (en) * 2017-10-23 2018-04-27 宁波大学 Glass slide soaks and cleaning device

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109443888A (en) * 2018-10-29 2019-03-08 重庆英特力科技有限公司 A kind of microbial staining device

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